RLG00000016862

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006904
Physical Location & Seq
Reverse (-)
10762290 .. 10765564
3275 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016862

Sequence Viewer

Length: 1683 bp
ATGGAGGAGAGGGAAGGTCGATCCTTGGCTGAAACACCCACATGGACTGTGGCTACTGTGATCACTGTCTTGATTTCGCTTGGGTTCTTCTTTCATTGCTCTTTGAAACATTTTGGAAAGTGGTTGGACAAGACTAAGAAGAAGTCTATACTCGCTGCTTTGGAAAAGATCCAAGAAGAGCTGATGCTTTTTGGGCTTCTGTCATTGTTGATGGGTCACTGGATTGTTTTTGTGGCCAAGATTTGTGTTAAATCTTCATCATGGGCCTTCAGCAGCCGGTTCTTTCCTTGTGCTTTGGAGCAAGAGATCAGTGTGATTCAACATGCACTTTTTGCGAGTCCGGAGTATCTGAACAAGTCAGTTTTTAGAGTGGAAGTGAAAACTATGTCTCATGAATATTGTCCTAAGGGTCATGAGTCCTTTGCTTCTTATGAAAGTCTTGAACAGCTTCACCGATTAATATTCGTCCTTGGCATGACTCACGTTGTTTACAGTTTCTTTGCCATTGTCATGGCCATGATTAAGATTTATAGCTGGAGAACATGGGAAAATGAAGCTAAAGCTAACGCCATTCTAACCTTAAAAGATACCAAAGAAACTAAGAGAATAAGAAGATTGTCGACATTCATAGTTCACCATGCATCTCATCCATGGAGCCAGCACAAGTATCTTGTTTGGCTGCTTTGTTTCAGCCGCCAGTTCTGGAGTTCTATAAACCGACCTTCATATATGGCTTTGCGATTTGGATTCATCACTGCTCATCAGCTTCCTTTGTCATATGATTTCCATAATTATATGATCCGGAGCATGGAGGAAGAATTTCGTGATATTGTTGGTATCAGTATACCTCTCTGGATATACGCCATATGTTGCATTATTCTAGATTTTCATGGAAGCAACATGTACTTTTGGCTTTCCTTCCTTCCAGCCATTTTGATCCTGCTTATCGGCACTAAACTGCATCAGATAGTGGTAAAATTGGCTGTGGAAATCATGGAAGAAAGTCCATATATGGAAAATCACCAGTTTAAACTAAGGGATGATCTCTTTTGGTTCAAGAGGCCCTGGCTTCTTTTACGGCTAATACAACTGATATCCTTCCAGAATTCATTCGAGATGGCTACTTTCCTCTGGTCTCTGTGGGAAATTAAACATCCTTCATGTTTCATGGACAATCATACTTTCCTCGTGATCCGCTTGACATTTGGGGTAGTCTCCCAGTTCTGGTGTAGCTTCATTACATTCCCACTCTATGTTGTCATCACACAAATGGGTTCAAGCTTCAAGAAATCTGTAGTTTCCGAAAATGTAAGACAATTGCTATCTGGATGGAAAAGGAGAGTGAAGGCGAGACAAAGTACTTCATCTAATAAGACTGGACTCCTGAATTCATCATCAACATCAACAGTAGCATTCTCATCAGATTCTAGCAAAGTTTGTAGTTCTTTTTCCTCGAACAGTGTGGAAGGGAGCTCAGTCTCCTCCAGGGTCCAAAAAACATCTTCCCGACATCTAAAGTCCAATGTGCAGCAAGGTGAAGTTAGTGAGATCGATGAGAAGTCAGAGTCTAAGCTCTGCACGCAAACACTCTCTGCTTTGATGAAAGATCATGACATTTATGAAGAATATGAGGAAGATGAAGAAGAGGACTACCATGAGGATGATAACAGTATCAAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

561

Amino Acids

65.1

Weight (kDa)

7.95

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 6 - 471 3.9e-158 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 620, 844
AccIII TCCGGA 2 cut(s) 340, 801
AciI CCGC 2 cut(s) 694, 1195
AclWI GGATC 5 cut(s) 15, 163, 793, 931, 1186
AcoI YGGCCR 2 cut(s) 234, 513
AcsI RAATTY 3 cut(s) 818, 1105, 1387
AcuI CTGAAG 1 cut(s) 253
AfaI GTAC 2 cut(s) 905, 1360
AfiI CCNNNNNNNGG 2 cut(s) 808, 1224
AflIII ACRYGT 1 cut(s) 900
AgsI TTSAA 6 cut(s) 106, 320, 443, 1057, 1278, 1285
AjnI CCWGG 2 cut(s) 1064, 1484
Alw21I GWGCWC 1 cut(s) 1475
Alw26I GTCTC 5 cut(s) 393, 1140, 1219, 1345, 1483
AlwI GGATC 5 cut(s) 15, 163, 793, 931, 1186
Aor13HI TCCGGA 2 cut(s) 340, 801
AoxI GGCC 4 cut(s) 234, 264, 513, 1061
ApeKI GCWGC 4 cut(s) 155, 273, 679, 1528
ApoI RAATTY 3 cut(s) 818, 1105, 1387
AseI ATTAAT 1 cut(s) 458
Asp700I GAANNNNTTC 3 cut(s) 447, 819, 1109
AspS9I GGNCC 3 cut(s) 264, 1062, 1489
AsuHPI GGTGA 4 cut(s) 443, 626, 1013, 1547
AvaII GGWCC 1 cut(s) 1489
AxyI CCTNAGG 1 cut(s) 405
BalI TGGCCA 2 cut(s) 236, 515
BanII GRGCYC 1 cut(s) 1475
BauI CACGAG 1 cut(s) 1187
Bbv12I GWGCWC 1 cut(s) 1475
BbvI GCAGC 4 cut(s) 142, 285, 666, 1540
BccI CCATC 3 cut(s) 205, 1111, 1323
BceAI ACGGC 1 cut(s) 1094
BciT130I CCWGG 2 cut(s) 1066, 1486
BclI TGATCA 1 cut(s) 60
BcoDI GTCTC 5 cut(s) 393, 1140, 1219, 1345, 1483
BfaI CTAG 2 cut(s) 881, 1428
BfmI CTRYAG 1 cut(s) 1293
BisI GCNGC 5 cut(s) 156, 274, 680, 694, 1529
BlsI GCNGC 5 cut(s) 157, 275, 681, 695, 1530
BmcAI AGTACT 1 cut(s) 1360
Bme1390I CCNGG 2 cut(s) 1066, 1486
Bme18I GGWCC 1 cut(s) 1489
BmgT120I GGNCC 3 cut(s) 264, 1062, 1489
BmiI GGNNCC 2 cut(s) 656, 1490
BmrFI CCNGG 2 cut(s) 1066, 1486
BmrI ACTGGG 1 cut(s) 1213
BmsI GCATC 3 cut(s) 174, 650, 970
BmuI ACTGGG 1 cut(s) 1213
BpmI CTGGAG 3 cut(s) 556, 724, 1468
Bsa29I ATCGAT 1 cut(s) 1551
BsaI GGTCTC 1 cut(s) 1140
BsaJI CCNNGG 5 cut(s) 24, 469, 650, 1064, 1485
BsaWI WCCGGW 2 cut(s) 340, 801
Bsc4I CCNNNNNNNGG 2 cut(s) 808, 1224
Bse118I RCCGGY 1 cut(s) 276
Bse1I ACTGG 5 cut(s) 224, 697, 1024, 1219, 1381
Bse21I CCTNAGG 1 cut(s) 405
Bse3DI GCAATG 1 cut(s) 94
BseAI TCCGGA 2 cut(s) 340, 801
BseBI CCWGG 2 cut(s) 1066, 1486
BseCI ATCGAT 1 cut(s) 1551
BseDI CCNNGG 5 cut(s) 24, 469, 650, 1064, 1485
BseGI GGATG 5 cut(s) 646, 1045, 1153, 1334, 1666
BseLI CCNNNNNNNGG 2 cut(s) 808, 1224
BseMI GCAATG 1 cut(s) 94
BseMII CTCAG 1 cut(s) 1488
BseNI ACTGG 5 cut(s) 224, 697, 1024, 1219, 1381
BseRI GAGGAG 2 cut(s) 20, 1471
BseXI GCAGC 4 cut(s) 142, 285, 666, 1540
BsgI GTGCAG 2 cut(s) 1547, 1561
BshFI GGCC 4 cut(s) 236, 266, 515, 1063
BshVI ATCGAT 1 cut(s) 1551
BsiHKAI GWGCWC 1 cut(s) 1475
BsiSI CCGG 3 cut(s) 277, 341, 802
BslI CCNNNNNNNGG 2 cut(s) 808, 1224
BsmAI GTCTC 5 cut(s) 393, 1140, 1219, 1345, 1483
BsmI GAATGC 1 cut(s) 1412
BsnI GGCC 4 cut(s) 236, 266, 515, 1063
Bso31I GGTCTC 1 cut(s) 1140
Bsp1286I GDGCHC 1 cut(s) 1475
Bsp13I TCCGGA 2 cut(s) 340, 801
Bsp19I CCATGG 1 cut(s) 650
BspACI CCGC 2 cut(s) 694, 1195
BspANI GGCC 4 cut(s) 236, 266, 515, 1063
BspCNI CTCAG 1 cut(s) 1487
BspDI ATCGAT 1 cut(s) 1551
BspEI TCCGGA 2 cut(s) 340, 801
BspHI TCATGA 3 cut(s) 391, 412, 1609
BspLI GGNNCC 2 cut(s) 656, 1490
BspPI GGATC 5 cut(s) 15, 163, 793, 931, 1186
BspQI GCTCTTC 1 cut(s) 171
BspTNI GGTCTC 1 cut(s) 1140
BsrDI GCAATG 1 cut(s) 94
BsrFI RCCGGY 1 cut(s) 276
BsrI ACTGG 5 cut(s) 224, 697, 1024, 1219, 1381
BssAI RCCGGY 1 cut(s) 276
BssECI CCNNGG 5 cut(s) 24, 469, 650, 1064, 1485
BssNAI GTATAC 1 cut(s) 845
BssSI CACGAG 1 cut(s) 1187
BssT1I CCWWGG 3 cut(s) 24, 469, 650
Bst1107I GTATAC 1 cut(s) 845
Bst2BI CACGAG 1 cut(s) 1187
Bst2UI CCWGG 2 cut(s) 1066, 1486
Bst4CI ACNGT 7 cut(s) 49, 58, 67, 494, 1408, 1460, 1670
Bst6I CTCTTC 2 cut(s) 171, 1638
BstAPI GCANNNNNTGC 1 cut(s) 332
BstC8I GCNNGC 2 cut(s) 659, 1580
BstDEI CTNAG 6 cut(s) 135, 405, 600, 1034, 1474, 1569
BstDSI CCRYGG 1 cut(s) 650
BstF5I GGATG 5 cut(s) 646, 1045, 1153, 1334, 1666
BstMAI GTCTC 5 cut(s) 393, 1140, 1219, 1345, 1483
BstMWI GCNNNNNNNGC 3 cut(s) 193, 332, 1579
BstNI CCWGG 2 cut(s) 1066, 1486
BstNSI RCATGY 2 cut(s) 326, 904
BstSCI CCNGG 2 cut(s) 1064, 1484
BstSFI CTRYAG 1 cut(s) 1293
BstV1I GCAGC 4 cut(s) 142, 285, 666, 1540
BstX2I RGATCY 1 cut(s) 168
BstXI CCANNNNNNTGG 1 cut(s) 511
BstYI RGATCY 1 cut(s) 168
BstZ17I GTATAC 1 cut(s) 845
Bsu15I ATCGAT 1 cut(s) 1551
Bsu36I CCTNAGG 1 cut(s) 405
BsuRI GGCC 4 cut(s) 236, 266, 515, 1063
BsuTUI ATCGAT 1 cut(s) 1551
BtgI CCRYGG 1 cut(s) 650
BtsCI GGATG 5 cut(s) 646, 1045, 1153, 1334, 1666
BtsI GCAGTG 1 cut(s) 753
BtsIMutI CAGTG 5 cut(s) 63, 217, 316, 753, 1465
Cac8I GCNNGC 2 cut(s) 659, 1580
CciI TCATGA 3 cut(s) 391, 412, 1609
Cfr10I RCCGGY 1 cut(s) 276
Cfr13I GGNCC 3 cut(s) 264, 1062, 1489
ClaI ATCGAT 1 cut(s) 1551
Csp6I GTAC 2 cut(s) 904, 1359
CviQI GTAC 2 cut(s) 904, 1359
DdeI CTNAG 6 cut(s) 135, 405, 600, 1034, 1474, 1569
DraI TTTAAA 1 cut(s) 1030
EaeI YGGCCR 2 cut(s) 234, 513
Eam1104I CTCTTC 2 cut(s) 171, 1638
EarI CTCTTC 2 cut(s) 171, 1638
Ecl136II GAGCTC 1 cut(s) 1473
Eco130I CCWWGG 3 cut(s) 24, 469, 650
Eco24I GRGCYC 1 cut(s) 1475
Eco31I GGTCTC 1 cut(s) 1140
Eco32I GATATC 1 cut(s) 1095
Eco47I GGWCC 1 cut(s) 1489
Eco53kI GAGCTC 1 cut(s) 1473
Eco57I CTGAAG 1 cut(s) 253
Eco81I CCTNAGG 1 cut(s) 405
EcoICRI GAGCTC 1 cut(s) 1473
EcoO109I RGGNCCY 1 cut(s) 1062
EcoRI GAATTC 2 cut(s) 1105, 1387
EcoRII CCWGG 2 cut(s) 1064, 1484
EcoRV GATATC 1 cut(s) 1095
EcoT14I CCWWGG 3 cut(s) 24, 469, 650
EcoT22I ATGCAT 1 cut(s) 643
EcoT38I GRGCYC 1 cut(s) 1475
ErhI CCWWGG 3 cut(s) 24, 469, 650
FauNDI CATATG 2 cut(s) 778, 866
FbaI TGATCA 1 cut(s) 60
FblI GTMKAC 2 cut(s) 620, 844
Fnu4HI GCNGC 5 cut(s) 156, 274, 680, 694, 1529
FokI GGATG 5 cut(s) 633, 1052, 1140, 1341, 1673
FriOI GRGCYC 1 cut(s) 1475
Fsp4HI GCNGC 5 cut(s) 156, 274, 680, 694, 1529
FspBI CTAG 2 cut(s) 881, 1428
GluI GCNGC 5 cut(s) 156, 274, 680, 694, 1529
GsuI CTGGAG 3 cut(s) 556, 724, 1468
HaeIII GGCC 4 cut(s) 236, 266, 515, 1063
HapII CCGG 3 cut(s) 277, 341, 802
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HindIII AAGCTT 1 cut(s) 1279
HinfI GANTC 8 cut(s) 316, 337, 416, 478, 747, 1380, 1424, 1565
HpaII CCGG 3 cut(s) 277, 341, 802
HphI GGTGA 4 cut(s) 443, 626, 1013, 1547
Hpy166II GTNNAC 4 cut(s) 490, 621, 634, 845
Hpy188I TCNGA 5 cut(s) 351, 966, 1303, 1423, 1564
Hpy8I GTNNAC 4 cut(s) 490, 621, 634, 845
HpyAV CCTTC 9 cut(s) 8, 277, 732, 928, 932, 1108, 1167, 1339, 1460
HpyCH4III ACNGT 7 cut(s) 49, 58, 67, 494, 1408, 1460, 1670
HpyCH4IV ACGT 1 cut(s) 483
HpyCH4V TGCA 6 cut(s) 326, 641, 873, 961, 1528, 1578
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 332, 1579
HpyF3I CTNAG 6 cut(s) 135, 405, 600, 1034, 1474, 1569
HpySE526I ACGT 1 cut(s) 483
Kpn2I TCCGGA 2 cut(s) 340, 801
Ksp22I TGATCA 1 cut(s) 60
LguI GCTCTTC 1 cut(s) 171
LmnI GCTCC 4 cut(s) 298, 654, 804, 1470
Lsp1109I GCAGC 4 cut(s) 142, 285, 666, 1540
LweI GCATC 3 cut(s) 174, 650, 970
MaeI CTAG 2 cut(s) 881, 1428
MaeII ACGT 1 cut(s) 483
MaeIII GTNAC 1 cut(s) 215
MfeI CAATTG 1 cut(s) 1316
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 1 cut(s) 1475
MlsI TGGCCA 2 cut(s) 236, 515
MluCI AATT 7 cut(s) 790, 818, 977, 1105, 1146, 1316, 1387
MluNI TGGCCA 2 cut(s) 236, 515
MlyI GAGTC 5 cut(s) 346, 425, 472, 1374, 1574
MmeI TCCRAC 1 cut(s) 105
Mox20I TGGCCA 2 cut(s) 236, 515
Mph1103I ATGCAT 1 cut(s) 643
MroI TCCGGA 2 cut(s) 340, 801
MroXI GAANNNNTTC 3 cut(s) 447, 819, 1109
MscI TGGCCA 2 cut(s) 236, 515
MseI TTAA 6 cut(s) 249, 458, 522, 581, 1029, 1149
MslI CAYNNNNRTG 5 cut(s) 40, 509, 515, 1268, 1659
Msp20I TGGCCA 2 cut(s) 236, 515
MspI CCGG 3 cut(s) 277, 341, 802
MspR9I CCNGG 2 cut(s) 1066, 1486
MssI GTTTAAAC 1 cut(s) 1030
MunI CAATTG 1 cut(s) 1316
Mva1269I GAATGC 1 cut(s) 1412
MvaI CCWGG 2 cut(s) 1066, 1486
MwoI GCNNNNNNNGC 3 cut(s) 193, 332, 1579
NcoI CCATGG 1 cut(s) 650
NdeI CATATG 2 cut(s) 778, 866
NlaIV GGNNCC 2 cut(s) 656, 1490
NmuCI GTSAC 1 cut(s) 215
NsiI ATGCAT 1 cut(s) 643
NspI RCATGY 2 cut(s) 326, 904
PagI TCATGA 3 cut(s) 391, 412, 1609
PciI ACATGT 1 cut(s) 900
PciSI GCTCTTC 1 cut(s) 171
PctI GAATGC 1 cut(s) 1412
PdmI GAANNNNTTC 3 cut(s) 447, 819, 1109
PfeI GAWTC 3 cut(s) 316, 747, 1424
PkrI GCNGC 5 cut(s) 157, 275, 681, 695, 1530
PleI GAGTC 5 cut(s) 345, 424, 472, 1374, 1573
PmeI GTTTAAAC 1 cut(s) 1030
PpsI GAGTC 5 cut(s) 345, 424, 472, 1374, 1573
PscI ACATGT 1 cut(s) 900
PshBI ATTAAT 1 cut(s) 458
Psp124BI GAGCTC 1 cut(s) 1475
Psp6I CCWGG 2 cut(s) 1064, 1484
PspGI CCWGG 2 cut(s) 1064, 1484
PspN4I GGNNCC 2 cut(s) 656, 1490
PspPI GGNCC 3 cut(s) 264, 1062, 1489
PsuI RGATCY 1 cut(s) 168
RsaI GTAC 2 cut(s) 905, 1360
RsaNI GTAC 2 cut(s) 904, 1359
RseI CAYNNNNRTG 5 cut(s) 40, 509, 515, 1268, 1659
SacI GAGCTC 1 cut(s) 1475
SalI GTCGAC 1 cut(s) 619
SapI GCTCTTC 1 cut(s) 171
SaqAI TTAA 6 cut(s) 249, 458, 522, 581, 1029, 1149
SatI GCNGC 5 cut(s) 156, 274, 680, 694, 1529
Sau96I GGNCC 3 cut(s) 264, 1062, 1489
ScaI AGTACT 1 cut(s) 1360
SchI GAGTC 5 cut(s) 346, 425, 472, 1374, 1574
ScrFI CCNGG 2 cut(s) 1066, 1486
SduI GDGCHC 1 cut(s) 1475
SfaNI GCATC 3 cut(s) 174, 650, 970
SfcI CTRYAG 1 cut(s) 1293
SinI GGWCC 1 cut(s) 1489
SmiMI CAYNNNNRTG 5 cut(s) 40, 509, 515, 1268, 1659
Sse9I AATT 7 cut(s) 790, 818, 977, 1105, 1146, 1316, 1387
SsiI CCGC 2 cut(s) 694, 1195
SspI AATATT 2 cut(s) 398, 462
SspMI CTAG 2 cut(s) 881, 1428
SstI GAGCTC 1 cut(s) 1475
StyD4I CCNGG 2 cut(s) 1064, 1484
StyI CCWWGG 3 cut(s) 24, 469, 650
TaaI ACNGT 7 cut(s) 49, 58, 67, 494, 1408, 1460, 1670
TaiI ACGT 1 cut(s) 486
TaqI TCGA 5 cut(s) 19, 620, 1113, 1454, 1551
TasI AATT 7 cut(s) 790, 818, 977, 1105, 1146, 1316, 1387
TatI WGTACW 2 cut(s) 903, 1358
TauI GCSGC 1 cut(s) 696
TfiI GAWTC 3 cut(s) 316, 747, 1424
Tru1I TTAA 6 cut(s) 249, 458, 522, 581, 1029, 1149
Tru9I TTAA 6 cut(s) 249, 458, 522, 581, 1029, 1149
TscAI CASTG 5 cut(s) 70, 224, 316, 760, 1465
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 4 cut(s) 155, 273, 679, 1528
Tsp45I GTSAC 1 cut(s) 215
TspRI CASTG 5 cut(s) 70, 224, 316, 760, 1465
VpaK11BI GGWCC 1 cut(s) 1489
VspI ATTAAT 1 cut(s) 458
XapI RAATTY 3 cut(s) 818, 1105, 1387
XbaI TCTAGA 1 cut(s) 880
XceI RCATGY 2 cut(s) 326, 904
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XmiI GTMKAC 2 cut(s) 620, 844
XmnI GAANNNNTTC 3 cut(s) 447, 819, 1109
XspI CTAG 2 cut(s) 881, 1428
ZrmI AGTACT 1 cut(s) 1360
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.