pycom02g10130

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0006904
Physical Location & Seq
Reverse (-)
7000830 .. 7004916
4087 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g10130.1

Sequence Viewer

Length: 1656 bp
ATGGAGGAGAGGGAAGGAAGATCCTTGGCTGAAACCCCAACATGGACAGTTGCTTGTGTGACTTGTGTCATGGTTGTTCTTGGGTTCTTCTTCAATGGCTCTTTGGAACGGGTTGGAAAGTGGTTGGATAAGACTAAAAGGAAGTCTCTAATTGCTGCTCTGCAGAAGATTAAAGAAGAGCTCATGCTTTTTGGGGTTATGTCATTGTTGATGAGTCACTTCATTATTATTGTGGCCAAGGTTTGTGTTAAATCGTCGGTTATGAGCAGCCGGTTCTTTCCATGTGCATCGGAGAATGAATTTTTGAATGCTGTTGAACATGTTCTTGTTCCGAGTTCAGGTAATTTGAATAGTTCAGTCTCCAGTGTACAGGTGAAGGCTGGTTCTCAACACGGCTATTGTCCTGAGGGTCATGAATCCTTTGCTTCTCAACAAAGTCTTGAACAGCTTCACCGTTTGATGTTTGTGCTCGGCATCACCCATGTTTTTTACAGCTTCGTAGCCATTGTCCTGGCCATGATTAAGATTTACAGCTGGAGAGTATGGGAAAATGAAGCCAAAGCTATAGCAATTCAGACCTTAGAAGATACTGCGGAAGGTAGACCAGGTATTCAAAGAATGAGGAGATTGTCTACTTTCATTTTTCACCATGCATCCCATCCATGGAGCCAGCACAGAGTTCTTGTTTGGCTGCTTTGTTTCAGCCGCCAGTTCTGGAGTTCTATAAACCGGTCTTCTTACATGGCTTTGCGCTTTGGCTTCATCACTACTCATCAACTTCCCTTGTCGTATGATTTCCATAATTATATGATTCGCAGCATGGAGGAAGAATTTCAGGATATTGTTGGTATCAGTTTGCCTCTTTGGATTTACACCATCTGTTGTGCTGTTCTAGATTTTCATGGAAGCAACCTTTACTTTTGGCTTTCCTTCCTTCCAGCCATTTTGATCCTGCTTATTGGTACAAAACTGCACCGAATAGTGGTAAAGCTGGCTGTCGAAATCATGGAAGAAAGTCCGTACATGAAAAATCATCAGTTTAAGCTAAGAGATGAGCTCTTCTGGTTTAAGAAGCCCTGGCTTCTTTTACGTGCAATACAATTGATATCCTTCCAGAATGCCCTCGAGATGGCAACTTTCATCTGGTCCCTGTGGGAAATTAAGGAACCTTCGTGTTTCATGGACAACCGTACTTTCCTTGTGATTCGCTTGACATTTGGGGTTGTCACGCAGTTCTGGTGTAGCTTTATAACCTTCCCACTCTATGTTATCATCACACAGATGGGTTCAAGGTTCAAGAAATCTGTAGTATCCGAAAATGTAAGAAATTCGCTACATGGATGGACAAGGAGAGTGAAGGCCAGACAAAGTACTTCATCCACTGCTACTACACACTTGTTGAAGACATCAACATCATCCGATTCTATTGTGGATGAAACTCGCAAACTCGAAAGTTCTGCCTCAAACTCTTCGGAGAGAAGTTCCTTCAAGAAACAGAATACATCTTTCCGACATCTGCAGGGCGGAGATGAGAAGTCAGAAACTCCGCTCTCTGTTGGTCCGGTTTACGACAGTTACAGCGATGACAACGAGGGTGATTACCATCACGATGATCATAGTAACATAAAACATGAATTGGCTCTCATTCCACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

552

Amino Acids

63.39

Weight (kDa)

8.56

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 6 - 471 1.4e-157 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1250
AccBSI CCGCTC 1 cut(s) 1549
AccI GTMKAC 2 cut(s) 601, 632
AciI CCGC 4 cut(s) 593, 706, 1524, 1547
AclWI GGATC 2 cut(s) 15, 943
AcoI YGGCCR 2 cut(s) 234, 513
AcsI RAATTY 3 cut(s) 299, 830, 1327
AfaI GTAC 5 cut(s) 369, 964, 1022, 1192, 1372
AfiI CCNNNNNNNGG 5 cut(s) 42, 338, 663, 982, 1129
AflIII ACRYGT 1 cut(s) 319
AgeI ACCGGT 1 cut(s) 729
AjnI CCWGG 3 cut(s) 510, 604, 1076
AluBI AGCT 9 cut(s) 181, 448, 495, 534, 563, 991, 1045, 1057, 1245
AluI AGCT 9 cut(s) 181, 448, 495, 534, 563, 991, 1045, 1057, 1245
Alw21I GWGCWC 3 cut(s) 183, 471, 1059
Alw26I GTCTC 2 cut(s) 150, 364
AlwI GGATC 2 cut(s) 15, 943
Ama87I CYCGRG 1 cut(s) 1124
AoxI GGCC 3 cut(s) 234, 513, 1359
ApeKI GCWGC 4 cut(s) 155, 267, 691, 816
ApoI RAATTY 3 cut(s) 299, 830, 1327
AsiGI ACCGGT 1 cut(s) 729
Asp700I GAANNNNTTC 3 cut(s) 321, 447, 831
AspLEI GCGC 1 cut(s) 753
AspS9I GGNCC 2 cut(s) 1146, 1559
AsuHPI GGTGA 5 cut(s) 385, 443, 469, 638, 1607
AvaI CYCGRG 1 cut(s) 1124
AvaII GGWCC 2 cut(s) 1146, 1559
AxyI CCTNAGG 1 cut(s) 405
BalI TGGCCA 2 cut(s) 236, 515
BanII GRGCYC 2 cut(s) 183, 1059
BbsI GAAGAC 2 cut(s) 726, 1409
Bbv12I GWGCWC 3 cut(s) 183, 471, 1059
BbvI GCAGC 4 cut(s) 142, 279, 678, 828
BccI CCATC 6 cut(s) 666, 884, 1123, 1276, 1335, 1611
BceAI ACGGC 1 cut(s) 409
BcgI CGANNNNNNTGC 2 cut(s) 1439, 1473
BciT130I CCWGG 3 cut(s) 512, 606, 1078
BciVI GTATCC 1 cut(s) 1321
BclI TGATCA 1 cut(s) 1612
BcoDI GTCTC 2 cut(s) 150, 364
BfaI CTAG 1 cut(s) 893
BfmI CTRYAG 4 cut(s) 161, 564, 1305, 1517
BfuI GTATCC 1 cut(s) 1321
BisI GCNGC 5 cut(s) 156, 268, 692, 706, 817
BlsI GCNGC 5 cut(s) 157, 269, 693, 707, 818
BmcAI AGTACT 1 cut(s) 1372
Bme1390I CCNGG 3 cut(s) 512, 606, 1078
Bme18I GGWCC 2 cut(s) 1146, 1559
BmeT110I CYCGRG 1 cut(s) 1124
BmgT120I GGNCC 2 cut(s) 1146, 1559
BmiI GGNNCC 3 cut(s) 668, 1148, 1167
BmrFI CCNGG 3 cut(s) 512, 606, 1078
BmsI GCATC 3 cut(s) 296, 483, 662
BoxI GACNNNNGTC 1 cut(s) 65
BpiI GAAGAC 2 cut(s) 726, 1409
BplI GAGNNNNNCTC 2 cut(s) 1041, 1073
BpmI CTGGAG 3 cut(s) 346, 556, 736
BsaAI YACGTR 1 cut(s) 1091
BsaBI GATNNNNATC 1 cut(s) 1602
BsaJI CCNNGG 4 cut(s) 24, 237, 662, 1076
BsaWI WCCGGW 2 cut(s) 729, 1561
Bsc4I CCNNNNNNNGG 5 cut(s) 42, 338, 663, 982, 1129
Bse118I RCCGGY 2 cut(s) 270, 729
Bse1I ACTGG 2 cut(s) 363, 709
Bse21I CCTNAGG 1 cut(s) 405
Bse8I GATNNNNATC 1 cut(s) 1602
BseBI CCWGG 3 cut(s) 512, 606, 1078
BseDI CCNNGG 4 cut(s) 24, 237, 662, 1076
BseGI GGATG 6 cut(s) 653, 658, 1346, 1376, 1415, 1438
BseJI GATNNNNATC 1 cut(s) 1602
BseLI CCNNNNNNNGG 5 cut(s) 42, 338, 663, 982, 1129
BseMII CTCAG 1 cut(s) 396
BseNI ACTGG 2 cut(s) 363, 709
BseRI GAGGAG 2 cut(s) 20, 637
BseXI GCAGC 4 cut(s) 142, 279, 678, 828
BsgI GTGCAG 1 cut(s) 956
BshFI GGCC 3 cut(s) 236, 515, 1361
BshTI ACCGGT 1 cut(s) 729
BsiHKAI GWGCWC 3 cut(s) 183, 471, 1059
BsiHKCI CYCGRG 1 cut(s) 1124
BsiSI CCGG 3 cut(s) 271, 730, 1562
BslFI GGGAC 1 cut(s) 1132
BslI CCNNNNNNNGG 5 cut(s) 42, 338, 663, 982, 1129
BsmAI GTCTC 2 cut(s) 150, 364
BsmFI GGGAC 1 cut(s) 1132
BsmI GAATGC 2 cut(s) 313, 1123
BsnI GGCC 3 cut(s) 236, 515, 1361
BsoBI CYCGRG 1 cut(s) 1124
Bsp1286I GDGCHC 3 cut(s) 183, 471, 1059
Bsp1407I TGTACA 1 cut(s) 367
Bsp143I GATC 3 cut(s) 20, 948, 1612
Bsp19I CCATGG 1 cut(s) 662
BspACI CCGC 4 cut(s) 593, 706, 1524, 1547
BspANI GGCC 3 cut(s) 236, 515, 1361
BspCNI CTCAG 1 cut(s) 397
BspHI TCATGA 1 cut(s) 412
BspLI GGNNCC 3 cut(s) 668, 1148, 1167
BspMAI CTGCAG 2 cut(s) 165, 1521
BspPI GGATC 2 cut(s) 15, 943
BspQI GCTCTTC 2 cut(s) 171, 1064
BsrBI CCGCTC 1 cut(s) 1549
BsrFI RCCGGY 2 cut(s) 270, 729
BsrGI TGTACA 1 cut(s) 367
BsrI ACTGG 2 cut(s) 363, 709
BssAI RCCGGY 2 cut(s) 270, 729
BssECI CCNNGG 4 cut(s) 24, 237, 662, 1076
BssMI GATC 3 cut(s) 20, 948, 1612
BssT1I CCWWGG 3 cut(s) 24, 237, 662
Bst2UI CCWGG 3 cut(s) 512, 606, 1078
Bst4CI ACNGT 4 cut(s) 49, 455, 1190, 1574
Bst6I CTCTTC 3 cut(s) 171, 1064, 1474
BstAUI TGTACA 1 cut(s) 367
BstBAI YACGTR 1 cut(s) 1091
BstC8I GCNNGC 2 cut(s) 671, 993
BstDEI CTNAG 3 cut(s) 405, 580, 1046
BstDSI CCRYGG 1 cut(s) 662
BstF5I GGATG 6 cut(s) 653, 658, 1346, 1376, 1415, 1438
BstHHI GCGC 1 cut(s) 753
BstKTI GATC 3 cut(s) 23, 951, 1615
BstMAI GTCTC 2 cut(s) 150, 364
BstMBI GATC 3 cut(s) 20, 948, 1612
BstNI CCWGG 3 cut(s) 512, 606, 1078
BstNSI RCATGY 1 cut(s) 323
BstPAI GACNNNNGTC 1 cut(s) 65
BstSCI CCNGG 3 cut(s) 510, 604, 1076
BstSFI CTRYAG 4 cut(s) 161, 564, 1305, 1517
BstV1I GCAGC 4 cut(s) 142, 279, 678, 828
BstV2I GAAGAC 2 cut(s) 726, 1409
BstX2I RGATCY 1 cut(s) 20
BstXI CCANNNNNNTGG 1 cut(s) 511
BstYI RGATCY 1 cut(s) 20
Bsu36I CCTNAGG 1 cut(s) 405
BsuI GTATCC 1 cut(s) 1321
BsuRI GGCC 3 cut(s) 236, 515, 1361
BtgI CCRYGG 1 cut(s) 662
BtgZI GCGATG 1 cut(s) 1596
BtsCI GGATG 6 cut(s) 653, 658, 1346, 1376, 1415, 1438
BtsI GCAGTG 1 cut(s) 1380
BtsIMutI CAGTG 2 cut(s) 370, 1380
Cac8I GCNNGC 2 cut(s) 671, 993
CciI TCATGA 1 cut(s) 412
CfoI GCGC 1 cut(s) 753
Cfr10I RCCGGY 2 cut(s) 270, 729
Cfr13I GGNCC 2 cut(s) 1146, 1559
CsiI ACCWGGT 1 cut(s) 604
Csp6I GTAC 5 cut(s) 368, 963, 1021, 1191, 1371
CspAI ACCGGT 1 cut(s) 729
CviQI GTAC 5 cut(s) 368, 963, 1021, 1191, 1371
DdeI CTNAG 3 cut(s) 405, 580, 1046
DpnI GATC 3 cut(s) 22, 950, 1614
DpnII GATC 3 cut(s) 20, 948, 1612
EaeI YGGCCR 2 cut(s) 234, 513
Eam1104I CTCTTC 3 cut(s) 171, 1064, 1474
EarI CTCTTC 3 cut(s) 171, 1064, 1474
EciI GGCGGA 1 cut(s) 1539
Ecl136II GAGCTC 2 cut(s) 181, 1057
Eco130I CCWWGG 3 cut(s) 24, 237, 662
Eco24I GRGCYC 2 cut(s) 183, 1059
Eco32I GATATC 1 cut(s) 1107
Eco47I GGWCC 2 cut(s) 1146, 1559
Eco53kI GAGCTC 2 cut(s) 181, 1057
Eco81I CCTNAGG 1 cut(s) 405
Eco88I CYCGRG 1 cut(s) 1124
EcoICRI GAGCTC 2 cut(s) 181, 1057
EcoRII CCWGG 3 cut(s) 510, 604, 1076
EcoRV GATATC 1 cut(s) 1107
EcoT14I CCWWGG 3 cut(s) 24, 237, 662
EcoT22I ATGCAT 1 cut(s) 655
EcoT38I GRGCYC 2 cut(s) 183, 1059
ErhI CCWWGG 3 cut(s) 24, 237, 662
FaqI GGGAC 1 cut(s) 1132
FbaI TGATCA 1 cut(s) 1612
FblI GTMKAC 2 cut(s) 601, 632
Fnu4HI GCNGC 5 cut(s) 156, 268, 692, 706, 817
FokI GGATG 6 cut(s) 640, 645, 1353, 1363, 1402, 1445
FriOI GRGCYC 2 cut(s) 183, 1059
Fsp4HI GCNGC 5 cut(s) 156, 268, 692, 706, 817
FspBI CTAG 1 cut(s) 893
GlaI GCGC 1 cut(s) 752
GluI GCNGC 5 cut(s) 156, 268, 692, 706, 817
GsuI CTGGAG 3 cut(s) 346, 556, 736
HaeIII GGCC 3 cut(s) 236, 515, 1361
HapII CCGG 3 cut(s) 271, 730, 1562
HhaI GCGC 1 cut(s) 753
Hin6I GCGC 1 cut(s) 751
HinP1I GCGC 1 cut(s) 751
HinfI GANTC 5 cut(s) 214, 416, 811, 1204, 1421
HpaII CCGG 3 cut(s) 271, 730, 1562
HphI GGTGA 5 cut(s) 385, 443, 469, 638, 1607
Hpy166II GTNNAC 4 cut(s) 368, 602, 633, 1567
Hpy188I TCNGA 8 cut(s) 292, 333, 576, 1315, 1420, 1474, 1511, 1540
Hpy8I GTNNAC 4 cut(s) 368, 602, 633, 1567
Hpy99I CGWCG 1 cut(s) 259
HpyCH4III ACNGT 4 cut(s) 49, 455, 1190, 1574
HpyCH4IV ACGT 1 cut(s) 1090
HpyCH4V TGCA 6 cut(s) 163, 287, 653, 973, 1094, 1519
HpyF3I CTNAG 3 cut(s) 405, 580, 1046
HpySE526I ACGT 1 cut(s) 1090
HspAI GCGC 1 cut(s) 751
Ksp22I TGATCA 1 cut(s) 1612
Kzo9I GATC 3 cut(s) 20, 948, 1612
LguI GCTCTTC 2 cut(s) 171, 1064
LmnI GCTCC 1 cut(s) 666
Lsp1109I GCAGC 4 cut(s) 142, 279, 678, 828
LweI GCATC 3 cut(s) 296, 483, 662
MabI ACCWGGT 1 cut(s) 604
MaeI CTAG 1 cut(s) 893
MaeII ACGT 1 cut(s) 1090
MaeIII GTNAC 5 cut(s) 58, 215, 1225, 1574, 1619
MalI GATC 3 cut(s) 22, 950, 1614
MbiI CCGCTC 1 cut(s) 1549
MboI GATC 3 cut(s) 20, 948, 1612
MfeI CAATTG 1 cut(s) 1100
MflI RGATCY 1 cut(s) 20
MhlI GDGCHC 3 cut(s) 183, 471, 1059
MlsI TGGCCA 2 cut(s) 236, 515
MluNI TGGCCA 2 cut(s) 236, 515
MlyI GAGTC 1 cut(s) 223
MmeI TCCRAC 3 cut(s) 94, 105, 1534
MnlI CCTC 8 cut(s) 3, 400, 615, 817, 870, 1133, 1471, 1585
Mox20I TGGCCA 2 cut(s) 236, 515
Mph1103I ATGCAT 1 cut(s) 655
MroXI GAANNNNTTC 3 cut(s) 321, 447, 831
MscI TGGCCA 2 cut(s) 236, 515
MseI TTAA 6 cut(s) 171, 249, 522, 1041, 1068, 1161
MslI CAYNNNNRTG 2 cut(s) 1280, 1608
Msp20I TGGCCA 2 cut(s) 236, 515
MspA1I CMGCKG 1 cut(s) 534
MspI CCGG 3 cut(s) 271, 730, 1562
MspR9I CCNGG 3 cut(s) 512, 606, 1078
MunI CAATTG 1 cut(s) 1100
Mva1269I GAATGC 2 cut(s) 313, 1123
MvaI CCWGG 3 cut(s) 512, 606, 1078
NcoI CCATGG 1 cut(s) 662
NdeII GATC 3 cut(s) 20, 948, 1612
NlaIV GGNNCC 3 cut(s) 668, 1148, 1167
NmeAIII GCCGAG 1 cut(s) 450
NmuCI GTSAC 3 cut(s) 58, 215, 1225
NsiI ATGCAT 1 cut(s) 655
NspI RCATGY 1 cut(s) 323
PaeR7I CTCGAG 1 cut(s) 1124
PagI TCATGA 1 cut(s) 412
PciI ACATGT 1 cut(s) 319
PciSI GCTCTTC 2 cut(s) 171, 1064
PctI GAATGC 2 cut(s) 313, 1123
PdmI GAANNNNTTC 3 cut(s) 321, 447, 831
PfeI GAWTC 4 cut(s) 416, 811, 1204, 1421
PinAI ACCGGT 1 cut(s) 729
PkrI GCNGC 5 cut(s) 157, 269, 693, 707, 818
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
Ppu21I YACGTR 1 cut(s) 1091
PscI ACATGT 1 cut(s) 319
PshAI GACNNNNGTC 1 cut(s) 65
PsiI TTATAA 1 cut(s) 1250
Psp124BI GAGCTC 2 cut(s) 183, 1059
Psp6I CCWGG 3 cut(s) 510, 604, 1076
PspGI CCWGG 3 cut(s) 510, 604, 1076
PspN4I GGNNCC 3 cut(s) 668, 1148, 1167
PspPI GGNCC 2 cut(s) 1146, 1559
PstI CTGCAG 2 cut(s) 165, 1521
PsuI RGATCY 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 534
RsaI GTAC 5 cut(s) 369, 964, 1022, 1192, 1372
RsaNI GTAC 5 cut(s) 368, 963, 1021, 1191, 1371
RseI CAYNNNNRTG 2 cut(s) 1280, 1608
SacI GAGCTC 2 cut(s) 183, 1059
SapI GCTCTTC 2 cut(s) 171, 1064
SaqAI TTAA 6 cut(s) 171, 249, 522, 1041, 1068, 1161
SatI GCNGC 5 cut(s) 156, 268, 692, 706, 817
Sau3AI GATC 3 cut(s) 20, 948, 1612
Sau96I GGNCC 2 cut(s) 1146, 1559
ScaI AGTACT 1 cut(s) 1372
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 3 cut(s) 512, 606, 1078
SduI GDGCHC 3 cut(s) 183, 471, 1059
SexAI ACCWGGT 1 cut(s) 604
SfaNI GCATC 3 cut(s) 296, 483, 662
SfcI CTRYAG 4 cut(s) 161, 564, 1305, 1517
Sfr274I CTCGAG 1 cut(s) 1124
SinI GGWCC 2 cut(s) 1146, 1559
SlaI CTCGAG 1 cut(s) 1124
SmiMI CAYNNNNRTG 2 cut(s) 1280, 1608
SmlI CTYRAG 1 cut(s) 1124
SmoI CTYRAG 1 cut(s) 1124
SsiI CCGC 4 cut(s) 593, 706, 1524, 1547
SspMI CTAG 1 cut(s) 893
SstI GAGCTC 2 cut(s) 183, 1059
StyD4I CCNGG 3 cut(s) 510, 604, 1076
StyI CCWWGG 3 cut(s) 24, 237, 662
TaaI ACNGT 4 cut(s) 49, 455, 1190, 1574
TaiI ACGT 1 cut(s) 1093
TaqI TCGA 3 cut(s) 999, 1125, 1449
TatI WGTACW 2 cut(s) 367, 1370
TauI GCSGC 1 cut(s) 708
TfiI GAWTC 4 cut(s) 416, 811, 1204, 1421
Tru1I TTAA 6 cut(s) 171, 249, 522, 1041, 1068, 1161
Tru9I TTAA 6 cut(s) 171, 249, 522, 1041, 1068, 1161
TscAI CASTG 2 cut(s) 370, 1387
TseFI GTSAC 3 cut(s) 58, 215, 1225
TseI GCWGC 4 cut(s) 155, 267, 691, 816
Tsp45I GTSAC 3 cut(s) 58, 215, 1225
TspGWI ACGGA 1 cut(s) 1008
TspRI CASTG 2 cut(s) 370, 1387
VpaK11BI GGWCC 2 cut(s) 1146, 1559
XapI RAATTY 3 cut(s) 299, 830, 1327
XbaI TCTAGA 1 cut(s) 892
XceI RCATGY 1 cut(s) 323
XhoI CTCGAG 1 cut(s) 1124
XmiI GTMKAC 2 cut(s) 601, 632
XmnI GAANNNNTTC 3 cut(s) 321, 447, 831
XspI CTAG 1 cut(s) 893
ZrmI AGTACT 1 cut(s) 1372
Zsp2I ATGCAT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.