MD02G1129400.v1.1

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006904
Physical Location & Seq
Reverse (-)
10459432 .. 10464379
4948 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1129400.v1.1.491

Sequence Viewer

Length: 1656 bp
ATGGAGGAGAGGGAAGGAAGATCCTTGGCTGAAACCCCAACATGGACGGTTGCTTGTGTGACCTGTGTCATGGTTGTTCTTGGGTTCTTCTTCAATGGCTCTTTGGAACGGGTTGGAAAGTGGTTGGATAAGACTAAAAGGAAGTCTCTAATTGCTGCTCTGCAGAAGATTAAAGAAGAGCTCATGCTTTTTGGGGTTATGTCATTGTTGATGAGTCACTTCATTATCATTGTGGCCAAGATTTGTGTTAAATCGTCGGTTATGAGCAGCCGGTTCTTTCCATGTGCATCGGAGAGTGAATTTTTGAATGCTGTTGAACATGTTCTTGTTCCGACTTCGGGTAATTTGAATAGTTCAGTCTCCAGAGTACAGGTGAAGGCTGGTTCTCAACACGGCTATTGTCCTGAGGGTCACGAATCCTTTGCTTCTCAACAAAGTCTTGAACAGCTTCACCGTTTGATGTTTGTGCTCGGCATCACCCATGTTTTTTACAGCTTCGTAGCCATTGTCCTAGCCATGATTAAGTTATACAGCTGGAGAGTATGGGAAAATGAAGCCAAAGAAGCAATTAAGACCTTAGAAGATACCGCGCAAGGTCAACCAGGTATTCAAAGAATGAGGAGATTGTCTACTTTCATATGTCACCACACATCCCATCCATGGAGTCAGCACAGAGTTCTTGTTTGGCTGCTTTGTTTCAGCCGCCAGTTCTGGAGTTCTATAAACCGGTCTTCTTACATGGCTTTGCGCTTCGGCTTCATCACTACTCATCAACTTCCTTTGTCGTATGATTTCCATAATTATATGATTCGCAGCATGGAGGAAGAATTTCGGGATATTGTTGGTGTCAGTTTGCCTCTTTGGATTTACACCATCTGTTGCGTTGTTCTAGATTTTCATGGAAGCAACCTTTACTTTTGGCTGTCCTTCCTTCCAGCCATTTTGATCCTGCTTATTGGTACAAAACTGCACCGAATAGTGGTAAAGCTGGCTGTCGAAATCATGGAAGAAAGTCCGTATATGGAAAATCATCAGTTTAAACTAAGAGATGAGCTCTTCTGGTTTAAGAAGCCCTGGCTTCTTTTACGTGTAATACAACTGATATCCTTCCAGAATGCCCTCGAGATGGCAACTTTCATCTGGTCCCTGTGGGAAATTAAGGAACCTTTGTGTTTCATGGACAACCGTACTTTCCTTGTGATTCGCTTGACATTTGGGGTTGTCACACAGTTCTGGTGTAGCTTTATAACATTCCCACTCTATGTTATCATCACACAGATGGGTTCAAGGTTCAAGAAATCTGTAGTATCCGAAAATGTAAGAAATTCGCTACATGGATGGAAAAGGAGAGTGAAGGCCAGACAAGGTACTTCATCCACTGCTACTACACACTTGGTGAGGACATCAACATCATCCGATTCTATTGTGGATGAAACTCACAAACTAGAAAGTTCTTCCTCAAACTCTTCGGAGAGAAGTTCCTTCAAGAAACAGAATACATCTTTCCGACATCTGCAGGGCGGAGATGAGAAGTTAGAAACTCCTCTCTCTGTTGGTCCAGTTTACGACAGTTATAGCGATGACAACGATGAGGGTGATCACCATCACGATGATCATAGTAACATAAAAGATGAATTGGCTCTCCTTCCACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

552

Amino Acids

63.56

Weight (kDa)

8.22

Isoelectric Point (pI)

44.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 6 - 472 3.9e-157 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1247
AccI GTMKAC 1 cut(s) 629
AccII CGCG 1 cut(s) 590
AciI CCGC 3 cut(s) 588, 703, 1521
AclWI GGATC 2 cut(s) 15, 940
AcoI YGGCCR 1 cut(s) 234
AcsI RAATTY 3 cut(s) 299, 827, 1324
AdeI CACNNNGTG 1 cut(s) 1396
AfaI GTAC 4 cut(s) 369, 961, 1189, 1369
AfiI CCNNNNNNNGG 5 cut(s) 42, 338, 660, 979, 1126
AflIII ACRYGT 2 cut(s) 319, 1087
AgeI ACCGGT 1 cut(s) 726
AgsI TTSAA 9 cut(s) 94, 307, 317, 349, 443, 611, 1287, 1294, 1486
AjnI CCWGG 2 cut(s) 601, 1073
AluBI AGCT 7 cut(s) 181, 448, 495, 534, 988, 1054, 1242
AluI AGCT 7 cut(s) 181, 448, 495, 534, 988, 1054, 1242
Alw21I GWGCWC 3 cut(s) 183, 471, 1056
Alw26I GTCTC 2 cut(s) 150, 364
AlwI GGATC 2 cut(s) 15, 940
Ama87I CYCGRG 1 cut(s) 1121
AoxI GGCC 2 cut(s) 234, 1356
ApeKI GCWGC 4 cut(s) 155, 267, 688, 813
ApoI RAATTY 3 cut(s) 299, 827, 1324
AsiGI ACCGGT 1 cut(s) 726
Asp700I GAANNNNTTC 3 cut(s) 321, 447, 828
AspLEI GCGC 2 cut(s) 592, 750
AspS9I GGNCC 2 cut(s) 1143, 1556
AsuHPI GGTGA 7 cut(s) 385, 443, 469, 635, 1408, 1592, 1607
AvaI CYCGRG 1 cut(s) 1121
AvaII GGWCC 2 cut(s) 1143, 1556
AxyI CCTNAGG 1 cut(s) 405
BalI TGGCCA 1 cut(s) 236
BanII GRGCYC 2 cut(s) 183, 1056
BbsI GAAGAC 1 cut(s) 723
Bbv12I GWGCWC 3 cut(s) 183, 471, 1056
BbvI GCAGC 4 cut(s) 142, 279, 675, 825
BccI CCATC 6 cut(s) 663, 881, 1120, 1273, 1332, 1611
BceAI ACGGC 1 cut(s) 409
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BciT130I CCWGG 2 cut(s) 603, 1075
BciVI GTATCC 1 cut(s) 1318
BclI TGATCA 2 cut(s) 1597, 1612
BcoDI GTCTC 2 cut(s) 150, 364
BfaI CTAG 3 cut(s) 512, 890, 1445
BfmI CTRYAG 3 cut(s) 161, 1302, 1514
BfuI GTATCC 1 cut(s) 1318
BisI GCNGC 5 cut(s) 156, 268, 689, 703, 814
BlsI GCNGC 5 cut(s) 157, 269, 690, 704, 815
Bme1390I CCNGG 2 cut(s) 603, 1075
Bme18I GGWCC 2 cut(s) 1143, 1556
BmeT110I CYCGRG 1 cut(s) 1121
BmgT120I GGNCC 2 cut(s) 1143, 1556
BmiI GGNNCC 2 cut(s) 1145, 1164
BmrFI CCNGG 2 cut(s) 603, 1075
BmsI GCATC 2 cut(s) 296, 483
BoxI GACNNNNGTC 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 723
BplI GAGNNNNNCTC 2 cut(s) 1038, 1070
BpmI CTGGAG 3 cut(s) 346, 556, 733
BsaAI YACGTR 1 cut(s) 1088
BsaBI GATNNNNATC 1 cut(s) 1602
BsaJI CCNNGG 3 cut(s) 24, 659, 1073
BsaWI WCCGGW 1 cut(s) 726
Bsc4I CCNNNNNNNGG 5 cut(s) 42, 338, 660, 979, 1126
Bse118I RCCGGY 2 cut(s) 270, 726
Bse1I ACTGG 2 cut(s) 706, 1559
Bse21I CCTNAGG 1 cut(s) 405
Bse8I GATNNNNATC 1 cut(s) 1602
BseBI CCWGG 2 cut(s) 603, 1075
BseDI CCNNGG 3 cut(s) 24, 659, 1073
BseGI GGATG 6 cut(s) 650, 655, 1343, 1373, 1412, 1435
BseJI GATNNNNATC 1 cut(s) 1602
BseLI CCNNNNNNNGG 5 cut(s) 42, 338, 660, 979, 1126
BseMII CTCAG 1 cut(s) 396
BseNI ACTGG 2 cut(s) 706, 1559
BseRI GAGGAG 3 cut(s) 20, 634, 1533
BseXI GCAGC 4 cut(s) 142, 279, 675, 825
BsgI GTGCAG 1 cut(s) 953
Bsh1236I CGCG 1 cut(s) 590
BshFI GGCC 2 cut(s) 236, 1358
BshTI ACCGGT 1 cut(s) 726
BsiHKAI GWGCWC 3 cut(s) 183, 471, 1056
BsiHKCI CYCGRG 1 cut(s) 1121
BsiSI CCGG 2 cut(s) 271, 727
BslFI GGGAC 1 cut(s) 1129
BslI CCNNNNNNNGG 5 cut(s) 42, 338, 660, 979, 1126
BsmAI GTCTC 2 cut(s) 150, 364
BsmFI GGGAC 1 cut(s) 1129
BsmI GAATGC 2 cut(s) 313, 1120
BsnI GGCC 2 cut(s) 236, 1358
BsoBI CYCGRG 1 cut(s) 1121
Bsp1286I GDGCHC 3 cut(s) 183, 471, 1056
Bsp143I GATC 4 cut(s) 20, 945, 1597, 1612
Bsp19I CCATGG 1 cut(s) 659
BspACI CCGC 3 cut(s) 588, 703, 1521
BspANI GGCC 2 cut(s) 236, 1358
BspCNI CTCAG 1 cut(s) 397
BspFNI CGCG 1 cut(s) 590
BspLI GGNNCC 2 cut(s) 1145, 1164
BspMAI CTGCAG 2 cut(s) 165, 1518
BspPI GGATC 2 cut(s) 15, 940
BspQI GCTCTTC 2 cut(s) 171, 1061
BsrFI RCCGGY 2 cut(s) 270, 726
BsrI ACTGG 2 cut(s) 706, 1559
BssAI RCCGGY 2 cut(s) 270, 726
BssECI CCNNGG 3 cut(s) 24, 659, 1073
BssMI GATC 4 cut(s) 20, 945, 1597, 1612
BssT1I CCWWGG 2 cut(s) 24, 659
Bst2UI CCWGG 2 cut(s) 603, 1075
Bst4CI ACNGT 5 cut(s) 49, 455, 1187, 1230, 1571
Bst6I CTCTTC 3 cut(s) 171, 1061, 1471
BstBAI YACGTR 1 cut(s) 1088
BstC8I GCNNGC 1 cut(s) 990
BstDEI CTNAG 3 cut(s) 405, 577, 1043
BstDSI CCRYGG 1 cut(s) 659
BstF5I GGATG 6 cut(s) 650, 655, 1343, 1373, 1412, 1435
BstFNI CGCG 1 cut(s) 590
BstHHI GCGC 2 cut(s) 592, 750
BstKTI GATC 4 cut(s) 23, 948, 1600, 1615
BstMAI GTCTC 2 cut(s) 150, 364
BstMBI GATC 4 cut(s) 20, 945, 1597, 1612
BstMWI GCNNNNNNNGC 1 cut(s) 563
BstNI CCWGG 2 cut(s) 603, 1075
BstNSI RCATGY 1 cut(s) 323
BstPAI GACNNNNGTC 1 cut(s) 65
BstSCI CCNGG 2 cut(s) 601, 1073
BstSFI CTRYAG 3 cut(s) 161, 1302, 1514
BstUI CGCG 1 cut(s) 590
BstV1I GCAGC 4 cut(s) 142, 279, 675, 825
BstV2I GAAGAC 1 cut(s) 723
BstX2I RGATCY 1 cut(s) 20
BstYI RGATCY 1 cut(s) 20
Bsu36I CCTNAGG 1 cut(s) 405
BsuI GTATCC 1 cut(s) 1318
BsuRI GGCC 2 cut(s) 236, 1358
BtgI CCRYGG 1 cut(s) 659
BtgZI GCGATG 1 cut(s) 1593
BtsCI GGATG 6 cut(s) 650, 655, 1343, 1373, 1412, 1435
BtsI GCAGTG 1 cut(s) 1377
BtsIMutI CAGTG 1 cut(s) 1377
Cac8I GCNNGC 1 cut(s) 990
CfoI GCGC 2 cut(s) 592, 750
Cfr10I RCCGGY 2 cut(s) 270, 726
Cfr13I GGNCC 2 cut(s) 1143, 1556
CsiI ACCWGGT 1 cut(s) 601
Csp6I GTAC 4 cut(s) 368, 960, 1188, 1368
CspAI ACCGGT 1 cut(s) 726
CviQI GTAC 4 cut(s) 368, 960, 1188, 1368
DdeI CTNAG 3 cut(s) 405, 577, 1043
DpnI GATC 4 cut(s) 22, 947, 1599, 1614
DpnII GATC 4 cut(s) 20, 945, 1597, 1612
DraI TTTAAA 1 cut(s) 1039
DraIII CACNNNGTG 1 cut(s) 1396
EaeI YGGCCR 1 cut(s) 234
Eam1104I CTCTTC 3 cut(s) 171, 1061, 1471
EarI CTCTTC 3 cut(s) 171, 1061, 1471
EciI GGCGGA 1 cut(s) 1536
Ecl136II GAGCTC 2 cut(s) 181, 1054
Eco130I CCWWGG 2 cut(s) 24, 659
Eco24I GRGCYC 2 cut(s) 183, 1056
Eco32I GATATC 1 cut(s) 1104
Eco47I GGWCC 2 cut(s) 1143, 1556
Eco53kI GAGCTC 2 cut(s) 181, 1054
Eco81I CCTNAGG 1 cut(s) 405
Eco88I CYCGRG 1 cut(s) 1121
EcoICRI GAGCTC 2 cut(s) 181, 1054
EcoRII CCWGG 2 cut(s) 601, 1073
EcoRV GATATC 1 cut(s) 1104
EcoT14I CCWWGG 2 cut(s) 24, 659
EcoT38I GRGCYC 2 cut(s) 183, 1056
ErhI CCWWGG 2 cut(s) 24, 659
FaqI GGGAC 1 cut(s) 1129
FauNDI CATATG 1 cut(s) 638
FbaI TGATCA 2 cut(s) 1597, 1612
FblI GTMKAC 1 cut(s) 629
Fnu4HI GCNGC 5 cut(s) 156, 268, 689, 703, 814
FokI GGATG 6 cut(s) 637, 642, 1350, 1360, 1399, 1442
FriOI GRGCYC 2 cut(s) 183, 1056
Fsp4HI GCNGC 5 cut(s) 156, 268, 689, 703, 814
FspBI CTAG 3 cut(s) 512, 890, 1445
GlaI GCGC 2 cut(s) 591, 749
GluI GCNGC 5 cut(s) 156, 268, 689, 703, 814
GsuI CTGGAG 3 cut(s) 346, 556, 733
HaeIII GGCC 2 cut(s) 236, 1358
HapII CCGG 2 cut(s) 271, 727
HhaI GCGC 2 cut(s) 592, 750
Hin6I GCGC 2 cut(s) 590, 748
HinP1I GCGC 2 cut(s) 590, 748
HincII GTYRAC 1 cut(s) 599
HindII GTYRAC 1 cut(s) 599
HinfI GANTC 6 cut(s) 214, 416, 664, 808, 1201, 1418
HpaII CCGG 2 cut(s) 271, 727
HphI GGTGA 7 cut(s) 385, 443, 469, 635, 1408, 1592, 1607
Hpy166II GTNNAC 3 cut(s) 599, 630, 1564
Hpy188I TCNGA 6 cut(s) 292, 333, 1312, 1417, 1471, 1508
Hpy8I GTNNAC 3 cut(s) 599, 630, 1564
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 8 cut(s) 8, 370, 937, 941, 1117, 1348, 1492, 1655
HpyCH4III ACNGT 5 cut(s) 49, 455, 1187, 1230, 1571
HpyCH4IV ACGT 1 cut(s) 1087
HpyCH4V TGCA 4 cut(s) 163, 287, 970, 1516
HpyF10VI GCNNNNNNNGC 1 cut(s) 563
HpyF3I CTNAG 3 cut(s) 405, 577, 1043
HpySE526I ACGT 1 cut(s) 1087
HspAI GCGC 2 cut(s) 590, 748
Ksp22I TGATCA 2 cut(s) 1597, 1612
Kzo9I GATC 4 cut(s) 20, 945, 1597, 1612
LguI GCTCTTC 2 cut(s) 171, 1061
Lsp1109I GCAGC 4 cut(s) 142, 279, 675, 825
LweI GCATC 2 cut(s) 296, 483
MabI ACCWGGT 1 cut(s) 601
MaeI CTAG 3 cut(s) 512, 890, 1445
MaeII ACGT 1 cut(s) 1087
MaeIII GTNAC 6 cut(s) 58, 215, 410, 641, 1222, 1619
MalI GATC 4 cut(s) 22, 947, 1599, 1614
MboI GATC 4 cut(s) 20, 945, 1597, 1612
MflI RGATCY 1 cut(s) 20
MhlI GDGCHC 3 cut(s) 183, 471, 1056
MlsI TGGCCA 1 cut(s) 236
MluCI AATT 9 cut(s) 150, 299, 343, 567, 799, 827, 1155, 1324, 1634
MluNI TGGCCA 1 cut(s) 236
MlyI GAGTC 2 cut(s) 223, 673
MmeI TCCRAC 4 cut(s) 94, 105, 356, 1531
Mox20I TGGCCA 1 cut(s) 236
MroXI GAANNNNTTC 3 cut(s) 321, 447, 828
MscI TGGCCA 1 cut(s) 236
MseI TTAA 7 cut(s) 171, 249, 522, 570, 1038, 1065, 1158
MslI CAYNNNNRTG 2 cut(s) 1277, 1608
Msp20I TGGCCA 1 cut(s) 236
MspA1I CMGCKG 1 cut(s) 534
MspI CCGG 2 cut(s) 271, 727
MspR9I CCNGG 2 cut(s) 603, 1075
MssI GTTTAAAC 1 cut(s) 1039
Mva1269I GAATGC 2 cut(s) 313, 1120
MvaI CCWGG 2 cut(s) 603, 1075
MvnI CGCG 1 cut(s) 590
MwoI GCNNNNNNNGC 1 cut(s) 563
NcoI CCATGG 1 cut(s) 659
NdeI CATATG 1 cut(s) 638
NdeII GATC 4 cut(s) 20, 945, 1597, 1612
NlaIV GGNNCC 2 cut(s) 1145, 1164
NmeAIII GCCGAG 1 cut(s) 450
NmuCI GTSAC 5 cut(s) 58, 215, 410, 641, 1222
NspI RCATGY 1 cut(s) 323
PaeR7I CTCGAG 1 cut(s) 1121
PciI ACATGT 1 cut(s) 319
PciSI GCTCTTC 2 cut(s) 171, 1061
PctI GAATGC 2 cut(s) 313, 1120
PdmI GAANNNNTTC 3 cut(s) 321, 447, 828
PfeI GAWTC 4 cut(s) 416, 808, 1201, 1418
PinAI ACCGGT 1 cut(s) 726
PkrI GCNGC 5 cut(s) 157, 269, 690, 704, 815
PleI GAGTC 2 cut(s) 222, 672
PmeI GTTTAAAC 1 cut(s) 1039
PpsI GAGTC 2 cut(s) 222, 672
Ppu21I YACGTR 1 cut(s) 1088
PscI ACATGT 1 cut(s) 319
PshAI GACNNNNGTC 1 cut(s) 65
PsiI TTATAA 1 cut(s) 1247
Psp124BI GAGCTC 2 cut(s) 183, 1056
Psp6I CCWGG 2 cut(s) 601, 1073
PspGI CCWGG 2 cut(s) 601, 1073
PspN4I GGNNCC 2 cut(s) 1145, 1164
PspPI GGNCC 2 cut(s) 1143, 1556
PstI CTGCAG 2 cut(s) 165, 1518
PsuI RGATCY 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 534
RsaI GTAC 4 cut(s) 369, 961, 1189, 1369
RsaNI GTAC 4 cut(s) 368, 960, 1188, 1368
RseI CAYNNNNRTG 2 cut(s) 1277, 1608
SacI GAGCTC 2 cut(s) 183, 1056
SapI GCTCTTC 2 cut(s) 171, 1061
SaqAI TTAA 7 cut(s) 171, 249, 522, 570, 1038, 1065, 1158
SatI GCNGC 5 cut(s) 156, 268, 689, 703, 814
Sau3AI GATC 4 cut(s) 20, 945, 1597, 1612
Sau96I GGNCC 2 cut(s) 1143, 1556
SchI GAGTC 2 cut(s) 223, 673
ScrFI CCNGG 2 cut(s) 603, 1075
SduI GDGCHC 3 cut(s) 183, 471, 1056
SexAI ACCWGGT 1 cut(s) 601
SfaNI GCATC 2 cut(s) 296, 483
SfcI CTRYAG 3 cut(s) 161, 1302, 1514
Sfr274I CTCGAG 1 cut(s) 1121
SinI GGWCC 2 cut(s) 1143, 1556
SlaI CTCGAG 1 cut(s) 1121
SmiMI CAYNNNNRTG 2 cut(s) 1277, 1608
SmlI CTYRAG 1 cut(s) 1121
SmoI CTYRAG 1 cut(s) 1121
Sse9I AATT 9 cut(s) 150, 299, 343, 567, 799, 827, 1155, 1324, 1634
SsiI CCGC 3 cut(s) 588, 703, 1521
SspMI CTAG 3 cut(s) 512, 890, 1445
SstI GAGCTC 2 cut(s) 183, 1056
StyD4I CCNGG 2 cut(s) 601, 1073
StyI CCWWGG 2 cut(s) 24, 659
TaaI ACNGT 5 cut(s) 49, 455, 1187, 1230, 1571
TaiI ACGT 1 cut(s) 1090
TaqI TCGA 2 cut(s) 996, 1122
TasI AATT 9 cut(s) 150, 299, 343, 567, 799, 827, 1155, 1324, 1634
TatI WGTACW 1 cut(s) 367
TauI GCSGC 1 cut(s) 705
TfiI GAWTC 4 cut(s) 416, 808, 1201, 1418
Tru1I TTAA 7 cut(s) 171, 249, 522, 570, 1038, 1065, 1158
Tru9I TTAA 7 cut(s) 171, 249, 522, 570, 1038, 1065, 1158
TscAI CASTG 1 cut(s) 1384
TseFI GTSAC 5 cut(s) 58, 215, 410, 641, 1222
TseI GCWGC 4 cut(s) 155, 267, 688, 813
Tsp45I GTSAC 5 cut(s) 58, 215, 410, 641, 1222
TspGWI ACGGA 1 cut(s) 1005
TspRI CASTG 1 cut(s) 1384
VpaK11BI GGWCC 2 cut(s) 1143, 1556
XapI RAATTY 3 cut(s) 299, 827, 1324
XbaI TCTAGA 1 cut(s) 889
XceI RCATGY 1 cut(s) 323
XhoI CTCGAG 1 cut(s) 1121
XmiI GTMKAC 1 cut(s) 629
XmnI GAANNNNTTC 3 cut(s) 321, 447, 828
XspI CTAG 3 cut(s) 512, 890, 1445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.