FvH4_1g12250

N-terminal region of micro-spherule protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010517
Physical Location & Seq
Reverse (-)
6708209 .. 6712446
4238 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g12250.t3

Sequence Viewer

Length: 2271 bp
ATGGCAGCGACGGCTCCGGCGCCGCCGTGGATCCCCGAAGACGACCTCCTCTTAAAGAACGCCATCGAGGCAGGTGCTACTCTGGAAGCGCTTGCCAAAGGAGCGGTCCGATTCTCCCGCAAGTTCACGGTGCGGGAGTTACGGGAGCGGTGGCGCTCGCTGCTCTACGACGCTGACGTGGCGGCGGAAGCCTCTTCTCGGATGGTGGAGTTCGAGGCGTGTGGCTCCAACGTCGTGCCGTGCAAGTCGAATAGGTTCAGTAGTCAATCTAGGGGTTCGAAACGGAAAGGAGAGAGCATTAGGAAGCAGTATTATGCTTTGAAGAAGAGGCTGTGTGCTGATAGCAACTTTGGAAGTCTTAATGATTTCGATATTAATTTTCTTGGAGGGCAAGCTATTGGAAATCAGGGGACTTTGGATGCTAATTGTATGGTTGGAGGCGGCGTTGAGCCGGTTTTTAATGGCGATGGCGGGAGTGTGTTTCATGGAGGAGAGTGTGAGGCGCTTAAGAATCGTGGTGTGGATGGAGTTGAGAGGGAAGGTTGCGGTAATGAGTTTTTGGAACCGGTTCTTTTGGTGGCGACGAATGGATTGGGGGAGAATGCTTATATCCGGGATAATAGTGTTCATGAGGATTTATCTACTCGTGGGGTTAATGCAATGGATTATGGGAATTCGTTGGATACGGAAGATATAGGGGCGCATTTGTGGGATGTAGCTGCACCGGAAATGCCAGCGGAAGAGACATTGGTAGTTGCTAATGATGTGGATGTCGATGGGGATACTATGGACATAATGGACTCGTCTCAATATGAAGTTGGCCATAATGAGGCAATGTTGGTTGATGGAGAAGGGTGCGATGCACTGAATAGGGCTGCAGCTATATCGGGAGGTGATTATGGAGATATGGCAGATTCTCTTTTTAATGATGAGGTCAATTCGATTTTGTTGAGTTCTCCCAAAGATGTTCATGACGATGATGTGCCTGAATTCTGTGAACCTGGAATGTTGGTGTCAGCCTCATTTGAGATTGTTTCAAATGATTTGCATACTGCTGAGATGGCTGTTTCTGTTGAACCATCTCAGACTGGTCACGATGATCTGCGAGATATTTTTTGTTCAGACGCCAATATGCCATCATCATCTACATCAATACCATTGAATCCTACCGAGATGACTGTTGCTCCCATGGTGCATGACGCTCCTGAGATGGCTGTTGCACCAGAGGTTCCAGAAGAGATGGTTTGTTCGTTGAATACTGAGGATCTTGACATTCCTTGCAATGATGATATCGTGTTTTTTACTGCAATTGTCCATACTGCCCTGCAACCGAGCTCTAACGAAGCTAGTAATTTGGGTACTGACCAAAGAAAATGTGATCAACATATAAAAACATTGGAGACAAAAGAAGATCCTGCACAACCTTTTAGAAAAGAAAGTCCAAACCCTACCCATGATAAAAATGACCTGGCTGCCGCAAGCCAATGCAGATTAGCAGATGCAACTCTCAACTGTATCACAAAGGGATTGCTAAACGAAGAGGAAATTAATGTTCCAGGTATAGTCGCAGAACTTGCATCTTTAACTGTAGAGCCAGGTTCTTATAATGTGGCTTTTCCAGAACCGCAAACCAACCCCACCGCACTAGATTGTGAAGAATCTGAGGAAGAATCTGATGACGATGTTGATGATGATGAAGAGGATACTGACATACCGTATTACTCTGATATCGAAACAATGATACTTGAGATGGATTTATGTCCAGCTGATGAGGAGTCAGATATCAGTAGACGAGTCTTAAAGTATCAACATGAGGATGCTAAAAGAAACATTATAAGGTTGGAACAGTGTGCACAATCCTCCATGCGAAGAGCGCTTACATCTAAAGGCACACTTGCGGTATTGTATGATCACCATGTAAAGCATTATATCAAGAAAACTGAGGTGATAATAGGCAGAACAACAGAAGATAATCAAGTTGATATTGATTTGGGAGATAACAAAGTATCTAGACGGCAGGCTCTTATAAAGATGGATGCAGACGGTTCTTTCTCCTTGAAGAATCTTGGCAAGAGTTCAATTTTCTTGAATGGCAGAGAAATAGCTACTGGAAAGATTTTAAATTTTGCTTCGAGTAATTTGATCGAGATTAGGGAGATGGCATTTGTTTTTGAGATTAACCACATATCTGTGAAGCAGTATCTGGCAAAAATTGCCACGAAAAGTGAGGGACACGCCAATTTGGAATTGGCACCTGAGGGGGTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0002151 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0004857 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005844 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010521 GO:0010556 GO:0010558 GO:0010605 GO:0010639 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0016579 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030234 GO:0030425 GO:0031011 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033202 GO:0034046 GO:0034708 GO:0035097 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043025 GO:0043086 GO:0043170 GO:0043204 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044463 GO:0044464 GO:0044665 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051338 GO:0051348 GO:0051972 GO:0051974 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070603 GO:0070646 GO:0070647 GO:0070717 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097346 GO:0097447 GO:0097458 GO:0098772 GO:0120025 GO:0120038 GO:1900180 GO:1900182 GO:1901363 GO:1901564 GO:1902493 GO:1902494 GO:1903827 GO:1903829 GO:1904356 GO:1904357 GO:1904749 GO:1904751 GO:1904949 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
FHA MCRS_N

Protein Analysis

757

Amino Acids

82.37

Weight (kDa)

4.39

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCRS_N PF13325 10 - 72 2.1e-12 N-terminal region of micro-spherule protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1605, 1835, 2027
AarI CACCTGC 1 cut(s) 62
Acc36I ACCTGC 1 cut(s) 62
AccB1I GGYRCC 2 cut(s) 19, 2251
AccBSI CCGCTC 2 cut(s) 104, 148
AccI GTMKAC 1 cut(s) 1789
AclWI GGATC 4 cut(s) 25, 38, 1272, 1406
AcoI YGGCCR 1 cut(s) 820
AcsI RAATTY 3 cut(s) 673, 989, 2122
AcyI GRCGYC 2 cut(s) 20, 1125
AfaI GTAC 1 cut(s) 1360
AfeI AGCGCT 2 cut(s) 90, 1875
AfiI CCNNNNNNNGG 2 cut(s) 198, 829
AflII CTTAAG 1 cut(s) 506
AgeI ACCGGT 1 cut(s) 565
AgsI TTSAA 8 cut(s) 322, 1038, 1076, 1162, 1255, 2059, 2079, 2089
AjiI CACGTC 1 cut(s) 178
AjnI CCWGG 4 cut(s) 1000, 1467, 1555, 1594
AluBI AGCT 7 cut(s) 395, 719, 881, 1335, 1346, 1766, 2105
AluI AGCT 7 cut(s) 395, 719, 881, 1335, 1346, 1766, 2105
Alw21I GWGCWC 2 cut(s) 1337, 1855
Alw26I GTCTC 3 cut(s) 737, 810, 1394
Alw44I GTGCAC 1 cut(s) 1851
AlwI GGATC 4 cut(s) 25, 38, 1272, 1406
AlwNI CAGNNNCTG 1 cut(s) 2203
Aor51HI AGCGCT 2 cut(s) 90, 1875
AoxI GGCC 1 cut(s) 820
ApaLI GTGCAC 1 cut(s) 1851
ApeKI GCWGC 6 cut(s) 5, 160, 719, 875, 878, 1472
ApoI RAATTY 3 cut(s) 673, 989, 2122
ArsI GACNNNNNNTTYG 2 cut(s) 90, 122
AseI ATTAAT 2 cut(s) 375, 1548
AsiGI ACCGGT 1 cut(s) 565
Asp700I GAANNNNTTC 2 cut(s) 254, 567
AspLEI GCGC 6 cut(s) 22, 91, 156, 505, 703, 1876
AspS9I GGNCC 1 cut(s) 106
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 3 cut(s) 905, 1904, 1957
AsuII TTCGAA 1 cut(s) 278
AvaII GGWCC 1 cut(s) 106
AxyI CCTNAGG 1 cut(s) 2256
BaeGI GKGCMC 1 cut(s) 1855
BalI TGGCCA 1 cut(s) 822
BamHI GGATCC 1 cut(s) 30
BanI GGYRCC 2 cut(s) 19, 2251
BanII GRGCYC 1 cut(s) 1337
BarI GAAGNNNNNNTAC 2 cut(s) 1861, 1893
BauI CACGAG 1 cut(s) 645
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 2 cut(s) 1337, 1855
BbvI GCAGC 6 cut(s) 17, 147, 706, 862, 890, 1459
BceAI ACGGC 4 cut(s) 10, 27, 223, 2030
BcgI CGANNNNNNTGC 4 cut(s) 56, 90, 867, 901
BciT130I CCWGG 4 cut(s) 1002, 1469, 1557, 1596
BciVI GTATCC 3 cut(s) 676, 775, 1696
BclI TGATCA 2 cut(s) 1378, 1909
BcnI CCSGG 1 cut(s) 614
BcoDI GTCTC 3 cut(s) 737, 810, 1394
BfaI CTAG 4 cut(s) 270, 1347, 1646, 2010
BfmI CTRYAG 2 cut(s) 876, 1587
BfoI RGCGCY 5 cut(s) 23, 92, 157, 506, 1877
BfrI CTTAAG 1 cut(s) 506
BfuAI ACCTGC 1 cut(s) 62
BfuI GTATCC 3 cut(s) 676, 775, 1696
BglI GCCNNNNNGGC 1 cut(s) 68
Bme1390I CCNGG 5 cut(s) 614, 1002, 1469, 1557, 1596
Bme18I GGWCC 1 cut(s) 106
BmgBI CACGTC 1 cut(s) 178
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 7 cut(s) 15, 21, 32, 226, 564, 1230, 2253
BmrFI CCNGG 5 cut(s) 614, 1002, 1469, 1557, 1596
BmsI GCATC 6 cut(s) 409, 850, 1489, 1586, 1807, 2026
BpiI GAAGAC 1 cut(s) 45
Bpu14I TTCGAA 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 1766
BpuMI CCSGG 1 cut(s) 614
BsaHI GRCGYC 2 cut(s) 20, 1125
BsaJI CCNNGG 2 cut(s) 26, 1188
BsaWI WCCGGW 2 cut(s) 565, 724
BsaXI ACNNNNNCTCC 4 cut(s) 429, 459, 1168, 1198
Bsc4I CCNNNNNNNGG 2 cut(s) 198, 829
Bse118I RCCGGY 2 cut(s) 451, 565
Bse1I ACTGG 2 cut(s) 1093, 2113
Bse21I CCTNAGG 1 cut(s) 2256
Bse3DI GCAATG 3 cut(s) 666, 840, 1288
BseBI CCWGG 4 cut(s) 1002, 1469, 1557, 1596
BseDI CCNNGG 2 cut(s) 26, 1188
BseGI GGATG 7 cut(s) 207, 424, 529, 718, 775, 1822, 2041
BseLI CCNNNNNNNGG 2 cut(s) 198, 829
BseMI GCAATG 3 cut(s) 666, 840, 1288
BseMII CTCAG 7 cut(s) 1047, 1097, 1197, 1251, 1653, 1932, 2247
BseNI ACTGG 2 cut(s) 1093, 2113
BseRI GAGGAG 3 cut(s) 38, 504, 1787
BseSI GKGCMC 1 cut(s) 1855
BseXI GCAGC 6 cut(s) 17, 147, 706, 862, 890, 1459
BsgI GTGCAG 2 cut(s) 705, 1401
BshFI GGCC 1 cut(s) 822
BshNI GGYRCC 2 cut(s) 19, 2251
BshTI ACCGGT 1 cut(s) 565
BsiHKAI GWGCWC 2 cut(s) 1337, 1855
BsiSI CCGG 5 cut(s) 17, 452, 566, 613, 725
BslFI GGGAC 2 cut(s) 424, 2244
BslI CCNNNNNNNGG 2 cut(s) 198, 829
BsmAI GTCTC 3 cut(s) 737, 810, 1394
BsmBI CGTCTC 1 cut(s) 810
BsmFI GGGAC 2 cut(s) 424, 2244
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 1 cut(s) 822
Bsp119I TTCGAA 1 cut(s) 278
Bsp1286I GDGCHC 2 cut(s) 1337, 1855
Bsp143I GATC 7 cut(s) 30, 1099, 1264, 1378, 1411, 1909, 2142
Bsp19I CCATGG 1 cut(s) 1188
BspANI GGCC 1 cut(s) 822
BspCNI CTCAG 7 cut(s) 1048, 1096, 1198, 1252, 1654, 1933, 2248
BspHI TCATGA 2 cut(s) 628, 970
BspLI GGNNCC 7 cut(s) 15, 21, 32, 226, 564, 1230, 2253
BspMAI CTGCAG 1 cut(s) 880
BspMI ACCTGC 1 cut(s) 62
BspPI GGATC 4 cut(s) 25, 38, 1272, 1406
BspQI GCTCTTC 1 cut(s) 1864
BspT104I TTCGAA 1 cut(s) 278
BspT107I GGYRCC 2 cut(s) 19, 2251
BspTI CTTAAG 1 cut(s) 506
BsrBI CCGCTC 2 cut(s) 104, 148
BsrDI GCAATG 3 cut(s) 666, 840, 1288
BsrFI RCCGGY 2 cut(s) 451, 565
BsrI ACTGG 2 cut(s) 1093, 2113
BssAI RCCGGY 2 cut(s) 451, 565
BssECI CCNNGG 2 cut(s) 26, 1188
BssMI GATC 7 cut(s) 30, 1099, 1264, 1378, 1411, 1909, 2142
BssNI GRCGYC 2 cut(s) 20, 1125
BssSI CACGAG 1 cut(s) 645
BssT1I CCWWGG 1 cut(s) 1188
Bst2BI CACGAG 1 cut(s) 645
Bst2UI CCWGG 4 cut(s) 1002, 1469, 1557, 1596
Bst4CI ACNGT 7 cut(s) 130, 1180, 1514, 1588, 1716, 1848, 2045
Bst6I CTCTTC 7 cut(s) 199, 320, 735, 1230, 1533, 1692, 1864
BstACI GRCGYC 2 cut(s) 20, 1125
BstAFI CTTAAG 1 cut(s) 506
BstAPI GCANNNNNTGC 2 cut(s) 1574, 2213
BstBI TTCGAA 1 cut(s) 278
BstC8I GCNNGC 6 cut(s) 93, 158, 393, 735, 1480, 2019
BstDEI CTNAG 7 cut(s) 1056, 1083, 1206, 1260, 1662, 1941, 2256
BstDSI CCRYGG 2 cut(s) 26, 1188
BstF5I GGATG 7 cut(s) 207, 424, 529, 718, 775, 1822, 2041
BstH2I RGCGCY 5 cut(s) 23, 92, 157, 506, 1877
BstHHI GCGC 6 cut(s) 22, 91, 156, 505, 703, 1876
BstKTI GATC 7 cut(s) 33, 1102, 1267, 1381, 1414, 1912, 2145
BstMAI GTCTC 3 cut(s) 737, 810, 1394
BstMBI GATC 7 cut(s) 30, 1099, 1264, 1378, 1411, 1909, 2142
BstNI CCWGG 4 cut(s) 1002, 1469, 1557, 1596
BstSCI CCNGG 5 cut(s) 612, 1000, 1467, 1555, 1594
BstSFI CTRYAG 2 cut(s) 876, 1587
BstSLI GKGCMC 1 cut(s) 1855
BstV1I GCAGC 6 cut(s) 17, 147, 706, 862, 890, 1459
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 3 cut(s) 30, 1264, 1411
BstYI RGATCY 3 cut(s) 30, 1264, 1411
Bsu36I CCTNAGG 1 cut(s) 2256
BsuI GTATCC 3 cut(s) 676, 775, 1696
BsuRI GGCC 1 cut(s) 822
BtgI CCRYGG 2 cut(s) 26, 1188
BtgZI GCGATG 2 cut(s) 480, 873
BtrI CACGTC 1 cut(s) 178
BtsCI GGATG 7 cut(s) 207, 424, 529, 718, 775, 1822, 2041
BtsIMutI CAGTG 2 cut(s) 863, 1853
BveI ACCTGC 1 cut(s) 62
Cac8I GCNNGC 6 cut(s) 93, 158, 393, 735, 1480, 2019
CaiI CAGNNNCTG 1 cut(s) 2203
CciI TCATGA 2 cut(s) 628, 970
CfoI GCGC 6 cut(s) 22, 91, 156, 505, 703, 1876
Cfr10I RCCGGY 2 cut(s) 451, 565
Cfr13I GGNCC 1 cut(s) 106
CpoI CGGWCCG 1 cut(s) 106
CseI GACGC 3 cut(s) 179, 1133, 1208
Csp6I GTAC 1 cut(s) 1359
CspAI ACCGGT 1 cut(s) 565
CspI CGGWCCG 1 cut(s) 106
CviAII CATG 9 cut(s) 485, 629, 971, 1189, 1196, 1454, 1811, 1864, 1916
CviQI GTAC 1 cut(s) 1359
DdeI CTNAG 7 cut(s) 1056, 1083, 1206, 1260, 1662, 1941, 2256
DinI GGCGCC 1 cut(s) 21
DpnI GATC 7 cut(s) 32, 1101, 1266, 1380, 1413, 1911, 2144
DpnII GATC 7 cut(s) 30, 1099, 1264, 1378, 1411, 1909, 2142
DraI TTTAAA 1 cut(s) 2121
EaeI YGGCCR 1 cut(s) 820
Eam1104I CTCTTC 7 cut(s) 199, 320, 735, 1230, 1533, 1692, 1864
EarI CTCTTC 7 cut(s) 199, 320, 735, 1230, 1533, 1692, 1864
EciI GGCGGA 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 1335
Eco130I CCWWGG 1 cut(s) 1188
Eco24I GRGCYC 1 cut(s) 1337
Eco32I GATATC 3 cut(s) 1291, 1729, 1783
Eco47I GGWCC 1 cut(s) 106
Eco47III AGCGCT 2 cut(s) 90, 1875
Eco53kI GAGCTC 1 cut(s) 1335
Eco81I CCTNAGG 1 cut(s) 2256
EcoICRI GAGCTC 1 cut(s) 1335
EcoRI GAATTC 2 cut(s) 673, 989
EcoRII CCWGG 4 cut(s) 1000, 1467, 1555, 1594
EcoRV GATATC 3 cut(s) 1291, 1729, 1783
EcoT14I CCWWGG 1 cut(s) 1188
EcoT38I GRGCYC 1 cut(s) 1337
EgeI GGCGCC 1 cut(s) 21
EheI GGCGCC 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 1188
Esp3I CGTCTC 1 cut(s) 810
FaeI CATG 9 cut(s) 488, 632, 974, 1192, 1199, 1457, 1814, 1867, 1919
FalI AAGNNNNNCTT 2 cut(s) 1878, 1910
FaqI GGGAC 2 cut(s) 424, 2244
FatI CATG 9 cut(s) 484, 628, 970, 1188, 1195, 1453, 1810, 1863, 1915
FauI CCCGC 3 cut(s) 125, 126, 464
FbaI TGATCA 2 cut(s) 1378, 1909
FblI GTMKAC 1 cut(s) 1789
FokI GGATG 7 cut(s) 214, 431, 536, 725, 782, 1829, 2048
FriOI GRGCYC 1 cut(s) 1337
FspBI CTAG 4 cut(s) 270, 1347, 1646, 2010
GlaI GCGC 6 cut(s) 21, 90, 155, 504, 702, 1875
HaeII RGCGCY 5 cut(s) 23, 92, 157, 506, 1877
HaeIII GGCC 1 cut(s) 822
HapII CCGG 5 cut(s) 17, 452, 566, 613, 725
HgaI GACGC 3 cut(s) 179, 1133, 1208
HhaI GCGC 6 cut(s) 22, 91, 156, 505, 703, 1876
Hin1I GRCGYC 2 cut(s) 20, 1125
Hin1II CATG 9 cut(s) 488, 632, 974, 1192, 1199, 1457, 1814, 1867, 1919
Hin6I GCGC 6 cut(s) 20, 89, 154, 503, 701, 1874
HinP1I GCGC 6 cut(s) 20, 89, 154, 503, 701, 1874
HpaII CCGG 5 cut(s) 17, 452, 566, 613, 725
HphI GGTGA 3 cut(s) 905, 1904, 1957
Hpy166II GTNNAC 4 cut(s) 126, 998, 1790, 1853
Hpy188I TCNGA 8 cut(s) 110, 201, 1086, 1123, 1663, 1675, 1726, 1780
Hpy8I GTNNAC 4 cut(s) 126, 998, 1790, 1853
Hpy99I CGWCG 4 cut(s) 13, 173, 236, 586
HpyAV CCTTC 2 cut(s) 533, 845
HpyCH4III ACNGT 7 cut(s) 130, 1180, 1514, 1588, 1716, 1848, 2045
HpyCH4IV ACGT 2 cut(s) 177, 231
HpyF3I CTNAG 7 cut(s) 1056, 1083, 1206, 1260, 1662, 1941, 2256
HpySE526I ACGT 2 cut(s) 177, 231
Hsp92I GRCGYC 2 cut(s) 20, 1125
Hsp92II CATG 9 cut(s) 488, 632, 974, 1192, 1199, 1457, 1814, 1867, 1919
HspAI GCGC 6 cut(s) 20, 89, 154, 503, 701, 1874
KasI GGCGCC 1 cut(s) 19
Ksp22I TGATCA 2 cut(s) 1378, 1909
Kzo9I GATC 7 cut(s) 30, 1099, 1264, 1378, 1411, 1909, 2142
LguI GCTCTTC 1 cut(s) 1864
LmnI GCTCC 6 cut(s) 19, 101, 145, 230, 1189, 1207
Lsp1109I GCAGC 6 cut(s) 17, 147, 706, 862, 890, 1459
LweI GCATC 6 cut(s) 409, 850, 1489, 1586, 1807, 2026
MaeI CTAG 4 cut(s) 270, 1347, 1646, 2010
MaeII ACGT 2 cut(s) 177, 231
MaeIII GTNAC 2 cut(s) 138, 1091
MalI GATC 7 cut(s) 32, 1101, 1266, 1380, 1413, 1911, 2144
MbiI CCGCTC 2 cut(s) 104, 148
MboI GATC 7 cut(s) 30, 1099, 1264, 1378, 1411, 1909, 2142
MfeI CAATTG 1 cut(s) 1308
MflI RGATCY 3 cut(s) 30, 1264, 1411
MhlI GDGCHC 2 cut(s) 1337, 1855
MlsI TGGCCA 1 cut(s) 822
MluNI TGGCCA 1 cut(s) 822
Mly113I GGCGCC 1 cut(s) 20
MlyI GAGTC 3 cut(s) 794, 1784, 1803
MmeI TCCRAC 4 cut(s) 252, 415, 660, 1821
Mox20I TGGCCA 1 cut(s) 822
MroXI GAANNNNTTC 2 cut(s) 254, 567
MscI TGGCCA 1 cut(s) 822
MslI CAYNNNNRTG 3 cut(s) 975, 1815, 2189
Msp20I TGGCCA 1 cut(s) 822
MspA1I CMGCKG 2 cut(s) 737, 1766
MspCI CTTAAG 1 cut(s) 506
MspI CCGG 5 cut(s) 17, 452, 566, 613, 725
MspR9I CCNGG 5 cut(s) 614, 1002, 1469, 1557, 1596
MunI CAATTG 1 cut(s) 1308
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 4 cut(s) 1002, 1469, 1557, 1596
NarI GGCGCC 1 cut(s) 20
NciI CCSGG 1 cut(s) 614
NcoI CCATGG 1 cut(s) 1188
NdeII GATC 7 cut(s) 30, 1099, 1264, 1378, 1411, 1909, 2142
NlaIII CATG 9 cut(s) 488, 632, 974, 1192, 1199, 1457, 1814, 1867, 1919
NlaIV GGNNCC 7 cut(s) 15, 21, 32, 226, 564, 1230, 2253
NmuCI GTSAC 1 cut(s) 1091
NspV TTCGAA 1 cut(s) 278
PagI TCATGA 2 cut(s) 628, 970
PaqCI CACCTGC 1 cut(s) 62
PciSI GCTCTTC 1 cut(s) 1864
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PctI GAATGC 1 cut(s) 607
PdmI GAANNNNTTC 2 cut(s) 254, 567
PfeI GAWTC 7 cut(s) 111, 511, 914, 1162, 1658, 1670, 2062
PfoI TCCNGGA 1 cut(s) 612
PinAI ACCGGT 1 cut(s) 565
PleI GAGTC 3 cut(s) 794, 1783, 1802
PluTI GGCGCC 1 cut(s) 23
PpsI GAGTC 3 cut(s) 794, 1783, 1802
PshBI ATTAAT 2 cut(s) 375, 1548
PsiI TTATAA 3 cut(s) 1605, 1835, 2027
Psp124BI GAGCTC 1 cut(s) 1337
Psp6I CCWGG 4 cut(s) 1000, 1467, 1555, 1594
PspGI CCWGG 4 cut(s) 1000, 1467, 1555, 1594
PspN4I GGNNCC 7 cut(s) 15, 21, 32, 226, 564, 1230, 2253
PspPI GGNCC 1 cut(s) 106
PstI CTGCAG 1 cut(s) 880
PstNI CAGNNNCTG 1 cut(s) 2203
PsuI RGATCY 3 cut(s) 30, 1264, 1411
PvuII CAGCTG 1 cut(s) 1766
RsaI GTAC 1 cut(s) 1360
RsaNI GTAC 1 cut(s) 1359
RseI CAYNNNNRTG 3 cut(s) 975, 1815, 2189
Rsr2I CGGWCCG 1 cut(s) 106
RsrII CGGWCCG 1 cut(s) 106
SacI GAGCTC 1 cut(s) 1337
SapI GCTCTTC 1 cut(s) 1864
Sau3AI GATC 7 cut(s) 30, 1099, 1264, 1378, 1411, 1909, 2142
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 3 cut(s) 794, 1784, 1803
ScrFI CCNGG 5 cut(s) 614, 1002, 1469, 1557, 1596
SduI GDGCHC 2 cut(s) 1337, 1855
SfaNI GCATC 6 cut(s) 409, 850, 1489, 1586, 1807, 2026
SfcI CTRYAG 2 cut(s) 876, 1587
SfoI GGCGCC 1 cut(s) 21
SfuI TTCGAA 1 cut(s) 278
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 3 cut(s) 975, 1815, 2189
SmlI CTYRAG 2 cut(s) 506, 1745
SmoI CTYRAG 2 cut(s) 506, 1745
SspDI GGCGCC 1 cut(s) 19
SspMI CTAG 4 cut(s) 270, 1347, 1646, 2010
SstI GAGCTC 1 cut(s) 1337
StyD4I CCNGG 5 cut(s) 612, 1000, 1467, 1555, 1594
StyI CCWWGG 1 cut(s) 1188
TaaI ACNGT 7 cut(s) 130, 1180, 1514, 1588, 1716, 1848, 2045
TaiI ACGT 2 cut(s) 180, 234
TauI GCSGC 4 cut(s) 25, 185, 444, 1478
TfiI GAWTC 7 cut(s) 111, 511, 914, 1162, 1658, 1670, 2062
TscAI CASTG 2 cut(s) 870, 1853
TseFI GTSAC 1 cut(s) 1091
TseI GCWGC 6 cut(s) 5, 160, 719, 875, 878, 1472
Tsp45I GTSAC 1 cut(s) 1091
TspDTI ATGAA 6 cut(s) 473, 617, 828, 959, 1710, 2256
TspGWI ACGGA 2 cut(s) 298, 701
TspRI CASTG 2 cut(s) 870, 1853
Vha464I CTTAAG 1 cut(s) 506
VneI GTGCAC 1 cut(s) 1851
VpaK11BI GGWCC 1 cut(s) 106
VspI ATTAAT 2 cut(s) 375, 1548
XapI RAATTY 3 cut(s) 673, 989, 2122
XbaI TCTAGA 1 cut(s) 2009
XcmI CCANNNNNNNNNTGG 1 cut(s) 2245
XmiI GTMKAC 1 cut(s) 1789
XmnI GAANNNNTTC 2 cut(s) 254, 567
XspI CTAG 4 cut(s) 270, 1347, 1646, 2010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.