MD15G1250100.v1.1

N-terminal region of micro-spherule protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010517
Physical Location & Seq
Reverse (-)
20907569 .. 20911982
4414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1250100.v1.1.491

Sequence Viewer

Length: 2475 bp
ATGGAAGCAGTTACTTCGGCTCTTCCGTGGATCCCGGAAGACGACCTCCGCTTGAAGGACGCCATGGAGACAGGTGCTTCTCTGGAAGCACTTGCCAGAGGCGCGGTGCGATTTTCGCGCAAATTCACGATCGGGGAGTTGCAGGATCGGTGGTACTCGCTCCTCTATGACGCCGATGTCTCGGCGGAGGCGTCGGCTCGGTTGGTGGAATTGGAAGGTTGCAATTCCAACTCAGCGTCCAAGGCTAGTAGATTCACTCGTTCTAGGGGTTTGAATCGAAAATCCAATAGCATACGAAAGCATTACTATGCTATGCGGAAGATAAATAGGAAACTGTGGGCTGTTAATTCCGTTGATCCCGATTCTTATCAGAAGGAGTTTTTCGATCGTAATTTTCTTTTCGGGCCGGATGTTGATAATGGCAAGGCTTTAGAGGAAAATTTTGGGGTTGGAGATCATAATCAGCCCGTGTTTTTGGACTGTGATGTGGATAACGGCAATGCCACTAATGCATTTCGTGGTGGAGAGTGTGTTTCTATTGGAAATCATGGGGTGGAGGGAGGTGGAGAGTGTGTTTCAATTGGAAATCATGGGGTAGAGGGAGTTGTGGGGGAAGGGTGCTCGAATGGATTTGTTGAACAGGTTTCATTATTGCCAAGTAACGGGTTAGGAGAGAATGCTTTTTGCCATGATAATACTTGTCATGATCTGCCTACTCACCGAGATGATGGAATTGATTTTGGGAATGCCTTAGATGCGGAAGATATAGGGCCATCTCATGCATCGATAGATGAGCCTCTTTGGAAAACGATTGAGGATGTACCGGCACCTGAAATGCCAATGGATGTCAGCATGGGGGTTAATGGTGAGGGTGCCCAAAAGACATTGGCAGGTACTGATGATATGGATGTGGATAACATTGGTTCATCTCAATATGAAGTTGTCCATTCAGAGGAAATGATAAATGATGAACTGAATAGGCCTATCATGATTTCCGGAGGTGATGATGCAGATATGTACCTTGCGAATGAAAATGAGCTTGCCTTCATGGATGTAAATGGCAAGGAGTCAATAGATAAGTCCTCTTCTGAAAACCTGAATCCGATTCTGTTGAGCGAGACCAAAGATGTTCATGAAAATGATGTACCCAACTTTTGTCAGCCCCCAAAGTTGGTTTCAGATCCATTCCAGGCTGTTACAGACAATGTGCAGGCTGCCAAGATGGATGTTGCTGCTGAACCGTCTCACTCTGACCTAGATGATCAGCATGTTATCTCTTGTTCTGAAGCCAATATGACAGCATCTACGTCAGTGCCACATCCTCTTACTCCTAAACATAATCATGAAGAGATGATTTGCACATTGAATACTGAGGACCCTGAAATCCCATGCAATGATGATATATTCCCATCTGCTGCAACTGTTCATGCTGTGGTGCAACCAGCCTTAAAAGGAGCTCATGAGTTGGCTTCCTCTACTGGAAAAAGGAATTACGATCAACAAAGGAAAGAAGAAGATCCTGCACGACCTTTTAAGGTTCCCCGGATGGTAGTATACGACACAAGTACAGAAAACAGCCCCAATCATGCACTTGGTCCTTTTGGAGTTAAAGCTCCATTTGGTGATAGTAACTGTGTAGCTTCAATCTCCAAACATGATAAAACTGTCATTGCCGACCAAAGCCAAAGCAGATCAGCACATGCACCAGCTAAGTCTATCACCAATCGAGTGTTCAAAGAAGAGGGATTAGAAGCTCCATTTACAATTGCAGAACTTGCACCCTTATTCGCAGAGCCAGGTTCTACGACTCTCCCAGAACCAGAAGCCAACCCATCAGCACTAGATCATGAAGAATCTGAGGAAGAATCTGATGATGATGTTGATGATGACGATGCTGACATTCCTTGTTTTTCTGATCTCGAAGCGATGATACTTGATATGGATTTATGTCCACTGGATCAGGATTCATACATCAGCAGTGAAGTCTTAGAGTATCAAAATGAGGATTCTAAAAGGAATATCATAAGGTTTGAACAATGCGCACAATCCTCTATGCAAAGAACCCTTGCATCTAAAGGTGCACTCGCTGTATTGTATGGTAACCATATAAAGAAGTACATTAAGAAAACTGAGGTAATAATTGGCAGATCAACAGAAGATAACGAAGTTGATATTGACTTGGGAAAGGAAGGGCTACATAACAAAATATCTCGGCGGCAGGCTCTTATAAAGATGGAAGGAGACGGTTCTTTCTATTTGAAGAATCTCGGCAAGAGTTCAATTTATTTGAATGGAGAAGAAGTTGCTACCGGGCAACTTGTTAGTCTTAGTTCAAGTAATTTGATTGAGATTAGGGAAATGTATTTTATTTTTGAGACGAATCACAAATCTGTGAGTCAGTATCTGGGAAGAATCGGCCAAAAAAGCGAGGATAAGTGCACCAAGTTGGAACGGTCGCCCGAGGGAGGTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0002151 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0004857 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005844 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010521 GO:0010556 GO:0010558 GO:0010605 GO:0010639 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0016579 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030234 GO:0030425 GO:0031011 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033202 GO:0034046 GO:0034708 GO:0035097 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043025 GO:0043086 GO:0043170 GO:0043204 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044463 GO:0044464 GO:0044665 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051338 GO:0051348 GO:0051972 GO:0051974 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070603 GO:0070646 GO:0070647 GO:0070717 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097346 GO:0097447 GO:0097458 GO:0098772 GO:0120025 GO:0120038 GO:1900180 GO:1900182 GO:1901363 GO:1901564 GO:1902493 GO:1902494 GO:1903827 GO:1903829 GO:1904356 GO:1904357 GO:1904749 GO:1904751 GO:1904949 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
FHA MCRS_N

Protein Analysis

825

Amino Acids

90.21

Weight (kDa)

4.56

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCRS_N PF13325 10 - 73 2.9e-13 N-terminal region of micro-spherule protein
FHA PF00498 712 - 784 4.9e-10 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2228
AasI GACNNNNNNGTC 1 cut(s) 176
Acc16I TGCGCA 1 cut(s) 2041
Acc36I ACCTGC 1 cut(s) 881
AccB1I GGYRCC 2 cut(s) 826, 872
AccI GTMKAC 1 cut(s) 1554
AccII CGCG 2 cut(s) 104, 118
AccIII TCCGGA 1 cut(s) 995
AciI CCGC 6 cut(s) 49, 104, 185, 316, 758, 2215
AclWI GGATC 7 cut(s) 25, 38, 153, 350, 1175, 1511, 1965
AcoI YGGCCR 1 cut(s) 2416
AcsI RAATTY 2 cut(s) 122, 439
AcuI CTGAAG 1 cut(s) 1305
AcyI GRCGYC 3 cut(s) 60, 171, 191
AfaI GTAC 7 cut(s) 155, 822, 895, 1019, 1146, 1567, 2117
AfiI CCNNNNNNNGG 5 cut(s) 55, 662, 952, 1171, 2465
AjnI CCWGG 2 cut(s) 1188, 1795
AjuI GAANNNNNNNTTGG 4 cut(s) 1159, 1191, 1715, 1747
AluBI AGCT 6 cut(s) 1039, 1457, 1613, 1640, 1709, 1754
AluI AGCT 6 cut(s) 1039, 1457, 1613, 1640, 1709, 1754
Alw21I GWGCWC 4 cut(s) 623, 1459, 2083, 2441
Alw26I GTCTC 6 cut(s) 62, 184, 1112, 1248, 2235, 2369
Alw44I GTGCAC 2 cut(s) 2079, 2437
AlwI GGATC 7 cut(s) 25, 38, 153, 350, 1175, 1511, 1965
AlwNI CAGNNNCTG 2 cut(s) 896, 2404
Ama87I CYCGRG 1 cut(s) 2459
Aor13HI TCCGGA 1 cut(s) 995
AoxI GGCC 4 cut(s) 404, 770, 980, 2416
ApaLI GTGCAC 2 cut(s) 2079, 2437
ApeKI GCWGC 3 cut(s) 1214, 1232, 1415
ApoI RAATTY 2 cut(s) 122, 439
AspLEI GCGC 3 cut(s) 104, 120, 2042
AspS9I GGNCC 5 cut(s) 404, 770, 1375, 1595, 2468
AsuC2I CCSGG 3 cut(s) 35, 1543, 2311
AsuHPI GGTGA 5 cut(s) 710, 878, 1013, 1634, 1711
AvaI CYCGRG 1 cut(s) 2459
AvaII GGWCC 3 cut(s) 1375, 1595, 2468
BaeGI GKGCMC 3 cut(s) 877, 2083, 2441
BamHI GGATCC 1 cut(s) 30
BanI GGYRCC 2 cut(s) 826, 872
BanII GRGCYC 1 cut(s) 1459
BarI GAAGNNNNNNTAC 2 cut(s) 1914, 1946
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 4 cut(s) 623, 1459, 2083, 2441
BbvI GCAGC 3 cut(s) 1201, 1219, 1402
BccI CCATC 7 cut(s) 722, 781, 1216, 1417, 1540, 1840, 2227
BceAI ACGGC 1 cut(s) 511
BcgI CGANNNNNNTGC 1 cut(s) 31
BciT130I CCWGG 2 cut(s) 1190, 1797
BclI TGATCA 1 cut(s) 1261
BcnI CCSGG 3 cut(s) 35, 1543, 2311
BcoDI GTCTC 6 cut(s) 62, 184, 1112, 1248, 2235, 2369
BfaI CTAG 4 cut(s) 246, 264, 1256, 1841
BfuAI ACCTGC 1 cut(s) 881
BisI GCNGC 4 cut(s) 1215, 1233, 1416, 2216
BlsI GCNGC 4 cut(s) 1216, 1234, 1417, 2217
Bme1390I CCNGG 5 cut(s) 35, 1190, 1543, 1797, 2311
Bme18I GGWCC 3 cut(s) 1375, 1595, 2468
BmeT110I CYCGRG 1 cut(s) 2459
BmgT120I GGNCC 5 cut(s) 404, 770, 1375, 1595, 2468
BmiI GGNNCC 5 cut(s) 32, 828, 874, 1377, 1539
BmrFI CCNGG 5 cut(s) 35, 1190, 1543, 1797, 2311
BmsI GCATC 6 cut(s) 745, 791, 997, 1310, 1882, 2078
BpiI GAAGAC 1 cut(s) 45
BpuMI CCSGG 3 cut(s) 35, 1543, 2311
Bsa29I ATCGAT 1 cut(s) 785
BsaBI GATNNNNATC 2 cut(s) 366, 459
BsaHI GRCGYC 3 cut(s) 60, 171, 191
BsaI GGTCTC 1 cut(s) 1112
BsaJI CCNNGG 5 cut(s) 26, 63, 240, 1541, 2460
BsaWI WCCGGW 1 cut(s) 995
BsaXI ACNNNNNCTCC 6 cut(s) 516, 546, 558, 588, 2286, 2316
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 662, 952, 1171, 2465
Bse118I RCCGGY 1 cut(s) 823
Bse1I ACTGG 2 cut(s) 1483, 1959
Bse3DI GCAATG 3 cut(s) 505, 1399, 1668
Bse8I GATNNNNATC 2 cut(s) 366, 459
BseAI TCCGGA 1 cut(s) 995
BseBI CCWGG 2 cut(s) 1190, 1797
BseCI ATCGAT 1 cut(s) 785
BseDI CCNNGG 5 cut(s) 26, 63, 240, 1541, 2460
BseGI GGATG 8 cut(s) 415, 823, 850, 913, 1057, 1231, 1318, 1551
BseJI GATNNNNATC 2 cut(s) 366, 459
BseLI CCNNNNNNNGG 5 cut(s) 55, 662, 952, 1171, 2465
BseMI GCAATG 3 cut(s) 505, 1399, 1668
BseMII CTCAG 4 cut(s) 246, 1362, 1848, 2121
BseNI ACTGG 2 cut(s) 1483, 1959
BseRI GAGGAG 1 cut(s) 152
BseSI GKGCMC 3 cut(s) 877, 2083, 2441
BseXI GCAGC 3 cut(s) 1201, 1219, 1402
BsgI GTGCAG 2 cut(s) 1229, 1506
Bsh1236I CGCG 2 cut(s) 104, 118
Bsh1285I CGRYCG 3 cut(s) 132, 388, 2456
BshFI GGCC 4 cut(s) 406, 772, 982, 2418
BshNI GGYRCC 2 cut(s) 826, 872
BshVI ATCGAT 1 cut(s) 785
BsiEI CGRYCG 3 cut(s) 132, 388, 2456
BsiHKAI GWGCWC 4 cut(s) 623, 1459, 2083, 2441
BsiHKCI CYCGRG 1 cut(s) 2459
BsiSI CCGG 6 cut(s) 35, 407, 824, 996, 1543, 2310
BslI CCNNNNNNNGG 5 cut(s) 55, 662, 952, 1171, 2465
BsmAI GTCTC 6 cut(s) 62, 184, 1112, 1248, 2235, 2369
BsmBI CGTCTC 3 cut(s) 1248, 2235, 2369
BsmI GAATGC 2 cut(s) 682, 751
BsnI GGCC 4 cut(s) 406, 772, 982, 2418
Bso31I GGTCTC 1 cut(s) 1112
BsoBI CYCGRG 1 cut(s) 2459
Bsp1286I GDGCHC 5 cut(s) 623, 877, 1459, 2083, 2441
Bsp13I TCCGGA 1 cut(s) 995
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 6 cut(s) 49, 104, 185, 316, 758, 2215
BspANI GGCC 4 cut(s) 406, 772, 982, 2418
BspCNI CTCAG 4 cut(s) 245, 1363, 1849, 2122
BspDI ATCGAT 1 cut(s) 785
BspEI TCCGGA 1 cut(s) 995
BspFNI CGCG 2 cut(s) 104, 118
BspHI TCATGA 6 cut(s) 703, 987, 1132, 1342, 1459, 1846
BspLI GGNNCC 5 cut(s) 32, 828, 874, 1377, 1539
BspMI ACCTGC 1 cut(s) 881
BspPI GGATC 7 cut(s) 25, 38, 153, 350, 1175, 1511, 1965
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 2 cut(s) 826, 872
BspTNI GGTCTC 1 cut(s) 1112
BsrDI GCAATG 3 cut(s) 505, 1399, 1668
BsrFI RCCGGY 1 cut(s) 823
BsrI ACTGG 2 cut(s) 1483, 1959
BssAI RCCGGY 1 cut(s) 823
BssECI CCNNGG 5 cut(s) 26, 63, 240, 1541, 2460
BssNAI GTATAC 1 cut(s) 1555
BssNI GRCGYC 3 cut(s) 60, 171, 191
BssT1I CCWWGG 2 cut(s) 63, 240
Bst1107I GTATAC 1 cut(s) 1555
Bst2UI CCWGG 2 cut(s) 1190, 1797
Bst4CI ACNGT 8 cut(s) 336, 482, 1242, 1423, 1634, 1666, 2246, 2454
Bst6I CTCTTC 4 cut(s) 27, 1090, 1341, 1734
BstACI GRCGYC 3 cut(s) 60, 171, 191
BstAPI GCANNNNNTGC 1 cut(s) 1775
BstC8I GCNNGC 3 cut(s) 1041, 1212, 2220
BstDEI CTNAG 8 cut(s) 232, 751, 1371, 1710, 1857, 1987, 2130, 2327
BstDSI CCRYGG 2 cut(s) 26, 63
BstEII GGTNACC 1 cut(s) 2099
BstF5I GGATG 8 cut(s) 415, 823, 850, 913, 1057, 1231, 1318, 1551
BstFNI CGCG 2 cut(s) 104, 118
BstHHI GCGC 3 cut(s) 104, 120, 2042
BstMAI GTCTC 6 cut(s) 62, 184, 1112, 1248, 2235, 2369
BstMCI CGRYCG 3 cut(s) 132, 388, 2456
BstMWI GCNNNNNNNGC 7 cut(s) 101, 115, 242, 509, 755, 1775, 2424
BstNI CCWGG 2 cut(s) 1190, 1797
BstNSI RCATGY 2 cut(s) 1271, 1703
BstPI GGTNACC 1 cut(s) 2099
BstSCI CCNGG 5 cut(s) 33, 1188, 1541, 1795, 2309
BstSLI GKGCMC 3 cut(s) 877, 2083, 2441
BstUI CGCG 2 cut(s) 104, 118
BstV1I GCAGC 3 cut(s) 1201, 1219, 1402
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 3 cut(s) 30, 1180, 1516
BstYI RGATCY 3 cut(s) 30, 1180, 1516
BstZ17I GTATAC 1 cut(s) 1555
Bsu15I ATCGAT 1 cut(s) 785
BsuRI GGCC 4 cut(s) 406, 772, 982, 2418
BsuTUI ATCGAT 1 cut(s) 785
BtgI CCRYGG 2 cut(s) 26, 63
BtgZI GCGATG 1 cut(s) 1940
BtsCI GGATG 8 cut(s) 415, 823, 850, 913, 1057, 1231, 1318, 1551
BtsI GCAGTG 1 cut(s) 1984
BtsIMutI CAGTG 3 cut(s) 1317, 1952, 1984
BveI ACCTGC 1 cut(s) 881
Cac8I GCNNGC 3 cut(s) 1041, 1212, 2220
CaiI CAGNNNCTG 2 cut(s) 896, 2404
CciI TCATGA 6 cut(s) 703, 987, 1132, 1342, 1459, 1846
CfoI GCGC 3 cut(s) 104, 120, 2042
Cfr10I RCCGGY 1 cut(s) 823
Cfr13I GGNCC 5 cut(s) 404, 770, 1375, 1595, 2468
ClaI ATCGAT 1 cut(s) 785
CseI GACGC 4 cut(s) 68, 179, 180, 225
Csp6I GTAC 7 cut(s) 154, 821, 894, 1018, 1145, 1566, 2116
CviQI GTAC 7 cut(s) 154, 821, 894, 1018, 1145, 1566, 2116
DdeI CTNAG 8 cut(s) 232, 751, 1371, 1710, 1857, 1987, 2130, 2327
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
EaeI YGGCCR 1 cut(s) 2416
Eam1104I CTCTTC 4 cut(s) 27, 1090, 1341, 1734
EarI CTCTTC 4 cut(s) 27, 1090, 1341, 1734
EciI GGCGGA 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 1457
Eco130I CCWWGG 2 cut(s) 63, 240
Eco147I AGGCCT 1 cut(s) 982
Eco24I GRGCYC 1 cut(s) 1459
Eco31I GGTCTC 1 cut(s) 1112
Eco47I GGWCC 3 cut(s) 1375, 1595, 2468
Eco53kI GAGCTC 1 cut(s) 1457
Eco57I CTGAAG 1 cut(s) 1305
Eco88I CYCGRG 1 cut(s) 2459
Eco91I GGTNACC 1 cut(s) 2099
EcoICRI GAGCTC 1 cut(s) 1457
EcoO109I RGGNCCY 1 cut(s) 1375
EcoO65I GGTNACC 1 cut(s) 2099
EcoRII CCWGG 2 cut(s) 1188, 1795
EcoT14I CCWWGG 2 cut(s) 63, 240
EcoT22I ATGCAT 2 cut(s) 514, 784
EcoT38I GRGCYC 1 cut(s) 1459
ErhI CCWWGG 2 cut(s) 63, 240
Esp3I CGTCTC 3 cut(s) 1248, 2235, 2369
FbaI TGATCA 1 cut(s) 1261
FblI GTMKAC 1 cut(s) 1554
Fnu4HI GCNGC 4 cut(s) 1215, 1233, 1416, 2216
FokI GGATG 8 cut(s) 422, 830, 857, 920, 1064, 1238, 1305, 1558
FriOI GRGCYC 1 cut(s) 1459
Fsp4HI GCNGC 4 cut(s) 1215, 1233, 1416, 2216
FspAI RTGCGCAY 1 cut(s) 2041
FspBI CTAG 4 cut(s) 246, 264, 1256, 1841
FspI TGCGCA 1 cut(s) 2041
GlaI GCGC 3 cut(s) 103, 119, 2041
GluI GCNGC 4 cut(s) 1215, 1233, 1416, 2216
HaeIII GGCC 4 cut(s) 406, 772, 982, 2418
HapII CCGG 6 cut(s) 35, 407, 824, 996, 1543, 2310
HgaI GACGC 4 cut(s) 68, 179, 180, 225
HhaI GCGC 3 cut(s) 104, 120, 2042
Hin1I GRCGYC 3 cut(s) 60, 171, 191
Hin6I GCGC 3 cut(s) 102, 118, 2040
HinP1I GCGC 3 cut(s) 102, 118, 2040
HpaII CCGG 6 cut(s) 35, 407, 824, 996, 1543, 2310
HphI GGTGA 5 cut(s) 710, 878, 1013, 1634, 1711
Hpy166II GTNNAC 4 cut(s) 1555, 1952, 2081, 2439
Hpy8I GTNNAC 4 cut(s) 1555, 1952, 2081, 2439
Hpy99I CGWCG 1 cut(s) 196
HpyAV CCTTC 7 cut(s) 49, 209, 367, 608, 1054, 2183, 2231
HpyCH4III ACNGT 8 cut(s) 336, 482, 1242, 1423, 1634, 1666, 2246, 2454
HpyCH4IV ACGT 1 cut(s) 1307
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 115, 242, 509, 755, 1775, 2424
HpyF3I CTNAG 8 cut(s) 232, 751, 1371, 1710, 1857, 1987, 2130, 2327
HpySE526I ACGT 1 cut(s) 1307
Hsp92I GRCGYC 3 cut(s) 60, 171, 191
HspAI GCGC 3 cut(s) 102, 118, 2040
Kpn2I TCCGGA 1 cut(s) 995
Ksp22I TGATCA 1 cut(s) 1261
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 4 cut(s) 165, 1454, 1618, 1759
Lsp1109I GCAGC 3 cut(s) 1201, 1219, 1402
LweI GCATC 6 cut(s) 745, 791, 997, 1310, 1882, 2078
MaeI CTAG 4 cut(s) 246, 264, 1256, 1841
MaeII ACGT 1 cut(s) 1307
MaeIII GTNAC 5 cut(s) 10, 659, 1195, 1628, 2099
MfeI CAATTG 2 cut(s) 579, 1764
MflI RGATCY 3 cut(s) 30, 1180, 1516
MhlI GDGCHC 5 cut(s) 623, 877, 1459, 2083, 2441
MlyI GAGTC 3 cut(s) 1076, 1801, 2404
MmeI TCCRAC 3 cut(s) 252, 430, 2427
Mph1103I ATGCAT 2 cut(s) 514, 784
MroI TCCGGA 1 cut(s) 995
MseI TTAA 6 cut(s) 345, 861, 1448, 1533, 1608, 2121
MslI CAYNNNNRTG 4 cut(s) 306, 723, 1137, 1341
MspI CCGG 6 cut(s) 35, 407, 824, 996, 1543, 2310
MspR9I CCNGG 5 cut(s) 35, 1190, 1543, 1797, 2311
MunI CAATTG 2 cut(s) 579, 1764
Mva1269I GAATGC 2 cut(s) 682, 751
MvaI CCWGG 2 cut(s) 1190, 1797
MvnI CGCG 2 cut(s) 104, 118
MwoI GCNNNNNNNGC 7 cut(s) 101, 115, 242, 509, 755, 1775, 2424
NciI CCSGG 3 cut(s) 35, 1543, 2311
NcoI CCATGG 1 cut(s) 63
NlaIV GGNNCC 5 cut(s) 32, 828, 874, 1377, 1539
NmeAIII GCCGAG 3 cut(s) 161, 2191, 2247
NsbI TGCGCA 1 cut(s) 2041
NsiI ATGCAT 2 cut(s) 514, 784
NspI RCATGY 2 cut(s) 1271, 1703
PagI TCATGA 6 cut(s) 703, 987, 1132, 1342, 1459, 1846
PceI AGGCCT 1 cut(s) 982
PciSI GCTCTTC 1 cut(s) 27
PcsI WCGNNNNNNNCGW 2 cut(s) 23, 188
PctI GAATGC 2 cut(s) 682, 751
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 4 cut(s) 1216, 1234, 1417, 2217
Ple19I CGATCG 2 cut(s) 132, 388
PleI GAGTC 3 cut(s) 1075, 1801, 2403
PpsI GAGTC 3 cut(s) 1075, 1801, 2403
PpuMI RGGWCCY 1 cut(s) 1375
PsiI TTATAA 1 cut(s) 2228
Psp124BI GAGCTC 1 cut(s) 1459
Psp5II RGGWCCY 1 cut(s) 1375
Psp6I CCWGG 2 cut(s) 1188, 1795
PspEI GGTNACC 1 cut(s) 2099
PspGI CCWGG 2 cut(s) 1188, 1795
PspN4I GGNNCC 5 cut(s) 32, 828, 874, 1377, 1539
PspPI GGNCC 5 cut(s) 404, 770, 1375, 1595, 2468
PspPPI RGGWCCY 1 cut(s) 1375
PstNI CAGNNNCTG 2 cut(s) 896, 2404
PsuI RGATCY 3 cut(s) 30, 1180, 1516
PvuI CGATCG 2 cut(s) 132, 388
RsaI GTAC 7 cut(s) 155, 822, 895, 1019, 1146, 1567, 2117
RsaNI GTAC 7 cut(s) 154, 821, 894, 1018, 1145, 1566, 2116
RseI CAYNNNNRTG 4 cut(s) 306, 723, 1137, 1341
SacI GAGCTC 1 cut(s) 1459
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 6 cut(s) 345, 861, 1448, 1533, 1608, 2121
SatI GCNGC 4 cut(s) 1215, 1233, 1416, 2216
Sau96I GGNCC 5 cut(s) 404, 770, 1375, 1595, 2468
SchI GAGTC 3 cut(s) 1076, 1801, 2404
ScrFI CCNGG 5 cut(s) 35, 1190, 1543, 1797, 2311
SduI GDGCHC 5 cut(s) 623, 877, 1459, 2083, 2441
SfaNI GCATC 6 cut(s) 745, 791, 997, 1310, 1882, 2078
SinI GGWCC 3 cut(s) 1375, 1595, 2468
SmiMI CAYNNNNRTG 4 cut(s) 306, 723, 1137, 1341
SseBI AGGCCT 1 cut(s) 982
SsiI CCGC 6 cut(s) 49, 104, 185, 316, 758, 2215
SspMI CTAG 4 cut(s) 246, 264, 1256, 1841
SstI GAGCTC 1 cut(s) 1459
StuI AGGCCT 1 cut(s) 982
StyD4I CCNGG 5 cut(s) 33, 1188, 1541, 1795, 2309
StyI CCWWGG 2 cut(s) 63, 240
TaaI ACNGT 8 cut(s) 336, 482, 1242, 1423, 1634, 1666, 2246, 2454
TaiI ACGT 1 cut(s) 1310
TaqI TCGA 6 cut(s) 277, 384, 623, 785, 1726, 1920
TatI WGTACW 2 cut(s) 1565, 2115
TauI GCSGC 1 cut(s) 2218
Tru1I TTAA 6 cut(s) 345, 861, 1448, 1533, 1608, 2121
Tru9I TTAA 6 cut(s) 345, 861, 1448, 1533, 1608, 2121
TscAI CASTG 3 cut(s) 1317, 1959, 1984
TseI GCWGC 3 cut(s) 1214, 1232, 1415
TspGWI ACGGA 2 cut(s) 15, 340
TspRI CASTG 3 cut(s) 1317, 1959, 1984
VneI GTGCAC 2 cut(s) 2079, 2437
VpaK11BI GGWCC 3 cut(s) 1375, 1595, 2468
XapI RAATTY 2 cut(s) 122, 439
XceI RCATGY 2 cut(s) 1271, 1703
XmiI GTMKAC 1 cut(s) 1554
XspI CTAG 4 cut(s) 246, 264, 1256, 1841
Zsp2I ATGCAT 2 cut(s) 514, 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.