Rw2G010450

N-terminal region of micro-spherule protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010517
Physical Location & Seq
Reverse (-)
10574835 .. 10579662
4828 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G010450.1

Sequence Viewer

Length: 2241 bp
ATGGCAGCGACGGCTTCGGCGCCGCCGTGGAGCCCGGAAGACGACCTCCTCCTCAAGAACGCCATCGAGGCAGGTGCTTCTCTGGAAGCGCTTGCCAGAGGAGCGGTCAGATTCTCCCGCAAATTCACGGTGGGAGATTTGCGGGAGCGGTGGCACGCGCTGCTCTACGACGCCGACGTGTCGTCGGAGGCGTCTGCTCGGATGTTGGAATTCGAGGCGTGTAATTCGAACGTCGTGCCGTCCAAGGCTAATAGGTTCAGTCAGTCTAGGGGTTCGAAACGGAGAGGGGAGAGCATAAGGAAGCAGTATCATGCTCTGAGGAAGAGGCTGTGTGCTGATACTCATCTCTGCACTCTTAGTGATTTCGATATTAATTTTCTTGGTGGGCAAGCTATTGGGAACCAGGGGAGTTTGGATGGGAATTGCATGGTTGGAGGTCATGTTGGGGAGCCGGTTTTCAATGGCGACAGTGGGAGTGTTTTTCATGGCGGAGAGTGTGTCTCGCTTGAGGATCATGGGGTAGGCGGAGTTGGGAGGGAAGGTTGTGGTAATGAGTTTTCGGAACAGGTTCTTTTAGTGGCGGCGAATGGATTGGGAGAGAATGCTTATTGCCGGGATAATAATGCCGGTGGGCATTTATCTACGCGCGGGGTTAATTCATTGGATTTCGGGAATTCGTTGGATACAGAAGATATAGGGGCACATTTATGGGACGTAGCAGCGCCAGAAATGCCAGCGGAAGAGACATTGGTAGTTGTTAATGATGGGGATGTCGATGGGGAGAATATGGATTCATCTCAATATGAAGTTGGCCATACTGAGGCAATGTTGGTTGATGGTGAAGGGTGTGATGAACTGAATAGGCGTGTAGCTATATCGGGAGGTGATTATGGAGATATGGAAGATTCTCTTTTCAATGATGAGGTCAATTCGATTCTGTTGAGTTCTCCCAAAGATGTTCATGAAGATGATGTTCCTGAATTCTGTCAACCTCCACAGTTGGTTTCAGTTTCATGTGAGGCTGTTTCAGATGTTGTGCATACTGCTGAGATGGTTCTTTCTGTCGAACCATCTCAGACTGGTCATGATGATCGGCTAGATATTTCATGTTCAGACGCCAATAAGCCATCATCATCTACATTGGTACCATTGAATCCTACTGAGATGACCATTGCTCCCATGGTGCCTGTTGCTTCTGAGATGGCCATTGCAGAAGAGGTTCAAGAAGAAGAGATGATTTGTACATTGAATACTGAGGATCCTGACATCCCTTCCAATGATGATATCATGTTTCCTACTGCGATTGTTCATACTTCCCTGCAACCGAGCTCTAAAGAAGTTAGTAATTTGGCTACTGACCAAACAAAAAGTGATCAACAAATAAAAACCTTGGAGAAAAAAGAAGATCCTGCACAACCTTTTAGAAAAGAAAGTCCAAACCCTGCACGTGATAAAAATGACCCTGCTGCCGCAAGCCAATGCAGATTAGCAGATGCAACTCTCAACTGTATCACAAAGGGATTGTTAAATGAAGAGGAAATTAATGTTCCAGGTCTAGTTGCAGAACTTGCATCTTTAACTGCAGAGCTAGGTTCTACTAGTATGGCTTTCCCAGAACCACAAACCAACCCCTCAGCACTAGATTGTGAAGAATCTGAGGAAGAATCTAATGACGATGTTGATGATGATGACGGGGACGCTGACATTCCTTATTTCTCTGATATCGAAACAATGATACTTGAGATGGATTTATGTCCAGCTGATGAGGAGTCAGATATCAGTAGACGAGTCTCAAAGTATCAACATGAGGATGCTAAAAGAAAAATTATAAGGTTGGAACAGTGTGCACAATCCTCTATGCAAAGAGCGCTTGCATCTAAAGGCACACTTGCTGTATTGTATGATCACCATGTAAAGCATTATATCAAGAAAACTGAGGTGATAATTGGCAGAACAACAGAAGATAATCAAGTTGATATTGATTTGGGAGATAACAAAGTATCTAGGCGGCAGGCTCTTATAAAGATGGATGCAGACGGTTCTTTCTCATTGAAGAATCTTGGCAAGAGTTCAATTTTCTTGAATGGCAGAGAAATTGCTACTGGAGAGCTTTTAAGTTTTGCTTCGAGTAATTTGATCGAGATTAGGGAGATGGCATTTGTTTTTGAGATTAACCACAAATCTGTGAGGCAGTATCTGGCAAAAATTGCCACAAAAAGTGAGGTACACACCAATTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0002151 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0004857 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005844 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010521 GO:0010556 GO:0010558 GO:0010605 GO:0010639 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0016579 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030234 GO:0030425 GO:0031011 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033202 GO:0034046 GO:0034708 GO:0035097 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043025 GO:0043086 GO:0043170 GO:0043204 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044463 GO:0044464 GO:0044665 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051338 GO:0051348 GO:0051972 GO:0051974 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070603 GO:0070646 GO:0070647 GO:0070717 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097346 GO:0097447 GO:0097458 GO:0098772 GO:0120025 GO:0120038 GO:1900180 GO:1900182 GO:1901363 GO:1901564 GO:1902493 GO:1902494 GO:1903827 GO:1903829 GO:1904356 GO:1904357 GO:1904749 GO:1904751 GO:1904949 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
FHA MCRS_N

Protein Analysis

746

Amino Acids

80.99

Weight (kDa)

4.47

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCRS_N PF13325 10 - 83 7.2e-14 N-terminal region of micro-spherule protein
FHA PF00498 647 - 701 1.8e-08 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1829, 2021
AarI CACCTGC 1 cut(s) 62
Acc36I ACCTGC 1 cut(s) 62
Acc65I GGTACC 1 cut(s) 1144
AccB1I GGYRCC 3 cut(s) 19, 1144, 1183
AccBSI CCGCTC 2 cut(s) 104, 148
AccI GTMKAC 1 cut(s) 1783
AccII CGCG 3 cut(s) 158, 646, 648
AclWI GGATC 4 cut(s) 519, 1253, 1266, 1400
AcoI YGGCCR 2 cut(s) 811, 1203
AcsI RAATTY 4 cut(s) 122, 209, 673, 980
AcvI CACGTG 1 cut(s) 1448
AcyI GRCGYC 4 cut(s) 20, 171, 191, 1116
AfaI GTAC 3 cut(s) 1146, 1243, 2226
AfeI AGCGCT 2 cut(s) 90, 1869
AfiI CCNNNNNNNGG 1 cut(s) 820
AflIII ACRYGT 1 cut(s) 177
AgsI TTSAA 8 cut(s) 460, 916, 1153, 1223, 1249, 2053, 2073, 2083
AhdI GACNNNNNGTC 1 cut(s) 181
AhlI ACTAGT 1 cut(s) 1598
AjiI CACGTC 1 cut(s) 178
AjnI CCWGG 2 cut(s) 402, 1549
AluBI AGCT 6 cut(s) 392, 872, 1329, 1588, 1760, 2110
AluI AGCT 6 cut(s) 392, 872, 1329, 1588, 1760, 2110
Alw21I GWGCWC 2 cut(s) 1331, 1849
Alw26I GTCTC 3 cut(s) 505, 737, 1795
Alw44I GTGCAC 1 cut(s) 1845
AlwI GGATC 4 cut(s) 519, 1253, 1266, 1400
AlwNI CAGNNNCTG 1 cut(s) 2197
Aor51HI AGCGCT 2 cut(s) 90, 1869
AoxI GGCC 2 cut(s) 811, 1203
ApaLI GTGCAC 1 cut(s) 1845
ApeKI GCWGC 4 cut(s) 5, 160, 719, 1466
ApoI RAATTY 4 cut(s) 122, 209, 673, 980
AseI ATTAAT 2 cut(s) 372, 1542
Asp700I GAANNNNTTC 2 cut(s) 567, 1218
Asp718I GGTACC 1 cut(s) 1144
AspLEI GCGC 6 cut(s) 22, 91, 160, 648, 724, 1870
AsuC2I CCSGG 2 cut(s) 35, 614
AsuHPI GGTGA 4 cut(s) 851, 896, 1898, 1951
AsuII TTCGAA 2 cut(s) 227, 275
BaeGI GKGCMC 2 cut(s) 703, 1849
BaeI ACNNNNGTAYC 2 cut(s) 1128, 1161
BalI TGGCCA 2 cut(s) 813, 1205
BamHI GGATCC 1 cut(s) 1258
BanI GGYRCC 3 cut(s) 19, 1144, 1183
BanII GRGCYC 2 cut(s) 35, 1331
BbrPI CACGTG 1 cut(s) 1448
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 2 cut(s) 1331, 1849
BbvCI CCTCAGC 1 cut(s) 1633
BbvI GCAGC 4 cut(s) 17, 147, 731, 1453
BceAI ACGGC 3 cut(s) 10, 27, 223
BcgI CGANNNNNNTGC 4 cut(s) 56, 90, 217, 251
BciT130I CCWGG 2 cut(s) 404, 1551
BciVI GTATCC 1 cut(s) 676
BclI TGATCA 2 cut(s) 1372, 1903
BcnI CCSGG 2 cut(s) 35, 614
BcoDI GTCTC 3 cut(s) 505, 737, 1795
BcuI ACTAGT 1 cut(s) 1598
BfaI CTAG 7 cut(s) 267, 1097, 1556, 1589, 1599, 1640, 2004
BfmI CTRYAG 1 cut(s) 1581
BfoI RGCGCY 4 cut(s) 23, 92, 725, 1871
BfuAI ACCTGC 1 cut(s) 62
BfuI GTATCC 1 cut(s) 676
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 8 cut(s) 6, 23, 161, 582, 720, 1467, 1470, 2009
BlsI GCNGC 8 cut(s) 7, 24, 162, 583, 721, 1468, 1471, 2010
Bme1390I CCNGG 4 cut(s) 35, 404, 614, 1551
BmeRI GACNNNNNGTC 1 cut(s) 181
BmgBI CACGTC 1 cut(s) 178
BmiI GGNNCC 7 cut(s) 21, 32, 401, 450, 1146, 1185, 1260
BmrFI CCNGG 4 cut(s) 35, 404, 614, 1551
BmsI GCATC 5 cut(s) 1483, 1580, 1801, 1883, 2020
BpiI GAAGAC 1 cut(s) 45
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpmI CTGGAG 1 cut(s) 2124
Bpu10I CCTNAGC 1 cut(s) 1633
Bpu14I TTCGAA 2 cut(s) 227, 275
BpuEI CTTGAG 3 cut(s) 38, 527, 1760
BpuMI CCSGG 2 cut(s) 35, 614
BsaAI YACGTR 1 cut(s) 1448
BsaBI GATNNNNATC 1 cut(s) 342
BsaHI GRCGYC 4 cut(s) 20, 171, 191, 1116
BsaJI CCNNGG 5 cut(s) 26, 243, 403, 1179, 1389
BsaXI ACNNNNNCTCC 8 cut(s) 426, 456, 483, 513, 526, 556, 1159, 1189
Bsc4I CCNNNNNNNGG 1 cut(s) 820
Bse118I RCCGGY 2 cut(s) 451, 626
Bse1I ACTGG 2 cut(s) 1084, 2107
Bse3DI GCAATG 3 cut(s) 831, 1170, 1206
Bse8I GATNNNNATC 1 cut(s) 342
BseBI CCWGG 2 cut(s) 404, 1551
BseDI CCNNGG 5 cut(s) 26, 243, 403, 1179, 1389
BseGI GGATG 6 cut(s) 207, 421, 775, 1266, 1816, 2035
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 1 cut(s) 820
BseMI GCAATG 3 cut(s) 831, 1170, 1206
BseNI ACTGG 2 cut(s) 1084, 2107
BseRI GAGGAG 4 cut(s) 38, 41, 114, 1781
BseSI GKGCMC 2 cut(s) 703, 1849
BseXI GCAGC 4 cut(s) 17, 147, 731, 1453
BsgI GTGCAG 3 cut(s) 334, 1395, 1428
Bsh1236I CGCG 3 cut(s) 158, 646, 648
BshFI GGCC 2 cut(s) 813, 1205
BshNI GGYRCC 3 cut(s) 19, 1144, 1183
BsiHKAI GWGCWC 2 cut(s) 1331, 1849
BsiSI CCGG 4 cut(s) 35, 452, 613, 627
BslFI GGGAC 2 cut(s) 725, 1709
BslI CCNNNNNNNGG 1 cut(s) 820
BsmAI GTCTC 3 cut(s) 505, 737, 1795
BsmFI GGGAC 2 cut(s) 725, 1709
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 2 cut(s) 813, 1205
Bsp119I TTCGAA 2 cut(s) 227, 275
Bsp1286I GDGCHC 4 cut(s) 35, 703, 1331, 1849
Bsp1407I TGTACA 1 cut(s) 1241
Bsp143I GATC 7 cut(s) 511, 1090, 1258, 1372, 1405, 1903, 2136
Bsp19I CCATGG 1 cut(s) 1179
BspANI GGCC 2 cut(s) 813, 1205
BspFNI CGCG 3 cut(s) 158, 646, 648
BspHI TCATGA 2 cut(s) 961, 1084
BspLI GGNNCC 7 cut(s) 21, 32, 401, 450, 1146, 1185, 1260
BspMAI CTGCAG 1 cut(s) 1585
BspMI ACCTGC 1 cut(s) 62
BspPI GGATC 4 cut(s) 519, 1253, 1266, 1400
BspT104I TTCGAA 2 cut(s) 227, 275
BspT107I GGYRCC 3 cut(s) 19, 1144, 1183
BsrBI CCGCTC 2 cut(s) 104, 148
BsrDI GCAATG 3 cut(s) 831, 1170, 1206
BsrFI RCCGGY 2 cut(s) 451, 626
BsrGI TGTACA 1 cut(s) 1241
BsrI ACTGG 2 cut(s) 1084, 2107
BssAI RCCGGY 2 cut(s) 451, 626
BssECI CCNNGG 5 cut(s) 26, 243, 403, 1179, 1389
BssMI GATC 7 cut(s) 511, 1090, 1258, 1372, 1405, 1903, 2136
BssNI GRCGYC 4 cut(s) 20, 171, 191, 1116
BssT1I CCWWGG 3 cut(s) 243, 1179, 1389
Bst2UI CCWGG 2 cut(s) 404, 1551
Bst4CI ACNGT 6 cut(s) 130, 470, 999, 1508, 1842, 2039
Bst6I CTCTTC 5 cut(s) 317, 735, 1209, 1224, 1527
BstACI GRCGYC 4 cut(s) 20, 171, 191, 1116
BstAPI GCANNNNNTGC 3 cut(s) 160, 1568, 2207
BstAUI TGTACA 1 cut(s) 1241
BstBAI YACGTR 1 cut(s) 1448
BstBI TTCGAA 2 cut(s) 227, 275
BstC8I GCNNGC 7 cut(s) 93, 156, 390, 735, 1474, 1872, 2013
BstDSI CCRYGG 2 cut(s) 26, 1179
BstF5I GGATG 6 cut(s) 207, 421, 775, 1266, 1816, 2035
BstFNI CGCG 3 cut(s) 158, 646, 648
BstH2I RGCGCY 4 cut(s) 23, 92, 725, 1871
BstHHI GCGC 6 cut(s) 22, 91, 160, 648, 724, 1870
BstKTI GATC 7 cut(s) 514, 1093, 1261, 1375, 1408, 1906, 2139
BstMAI GTCTC 3 cut(s) 505, 737, 1795
BstMBI GATC 7 cut(s) 511, 1090, 1258, 1372, 1405, 1903, 2136
BstMWI GCNNNNNNNGC 8 cut(s) 11, 68, 101, 160, 730, 1568, 1867, 2207
BstNI CCWGG 2 cut(s) 404, 1551
BstSCI CCNGG 4 cut(s) 33, 402, 612, 1549
BstSFI CTRYAG 1 cut(s) 1581
BstSLI GKGCMC 2 cut(s) 703, 1849
BstUI CGCG 3 cut(s) 158, 646, 648
BstV1I GCAGC 4 cut(s) 17, 147, 731, 1453
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 2 cut(s) 1258, 1405
BstYI RGATCY 2 cut(s) 1258, 1405
BsuI GTATCC 1 cut(s) 676
BsuRI GGCC 2 cut(s) 813, 1205
BtgI CCRYGG 2 cut(s) 26, 1179
BtrI CACGTC 1 cut(s) 178
BtsCI GGATG 6 cut(s) 207, 421, 775, 1266, 1816, 2035
BtsIMutI CAGTG 2 cut(s) 475, 1847
BveI ACCTGC 1 cut(s) 62
Cac8I GCNNGC 7 cut(s) 93, 156, 390, 735, 1474, 1872, 2013
CaiI CAGNNNCTG 1 cut(s) 2197
CciI TCATGA 2 cut(s) 961, 1084
CfoI GCGC 6 cut(s) 22, 91, 160, 648, 724, 1870
Cfr10I RCCGGY 2 cut(s) 451, 626
CseI GACGC 4 cut(s) 179, 180, 1124, 1706
Csp6I GTAC 3 cut(s) 1145, 1242, 2225
CviQI GTAC 3 cut(s) 1145, 1242, 2225
DinI GGCGCC 1 cut(s) 21
DpnI GATC 7 cut(s) 513, 1092, 1260, 1374, 1407, 1905, 2138
DpnII GATC 7 cut(s) 511, 1090, 1258, 1372, 1405, 1903, 2136
DriI GACNNNNNGTC 1 cut(s) 181
EaeI YGGCCR 2 cut(s) 811, 1203
Eam1104I CTCTTC 5 cut(s) 317, 735, 1209, 1224, 1527
Eam1105I GACNNNNNGTC 1 cut(s) 181
EarI CTCTTC 5 cut(s) 317, 735, 1209, 1224, 1527
EciI GGCGGA 2 cut(s) 504, 540
Ecl136II GAGCTC 1 cut(s) 1329
Eco130I CCWWGG 3 cut(s) 243, 1179, 1389
Eco24I GRGCYC 2 cut(s) 35, 1331
Eco32I GATATC 3 cut(s) 1285, 1723, 1777
Eco47III AGCGCT 2 cut(s) 90, 1869
Eco53kI GAGCTC 1 cut(s) 1329
Eco72I CACGTG 1 cut(s) 1448
EcoICRI GAGCTC 1 cut(s) 1329
EcoRI GAATTC 3 cut(s) 209, 673, 980
EcoRII CCWGG 2 cut(s) 402, 1549
EcoRV GATATC 3 cut(s) 1285, 1723, 1777
EcoT14I CCWWGG 3 cut(s) 243, 1179, 1389
EcoT38I GRGCYC 2 cut(s) 35, 1331
EgeI GGCGCC 1 cut(s) 21
EheI GGCGCC 1 cut(s) 21
ErhI CCWWGG 3 cut(s) 243, 1179, 1389
FalI AAGNNNNNCTT 6 cut(s) 894, 926, 1872, 1904, 2107, 2139
FaqI GGGAC 2 cut(s) 725, 1709
FauI CCCGC 3 cut(s) 125, 135, 641
FbaI TGATCA 2 cut(s) 1372, 1903
FblI GTMKAC 1 cut(s) 1783
Fnu4HI GCNGC 8 cut(s) 6, 23, 161, 582, 720, 1467, 1470, 2009
FokI GGATG 6 cut(s) 214, 428, 782, 1253, 1823, 2042
FriOI GRGCYC 2 cut(s) 35, 1331
Fsp4HI GCNGC 8 cut(s) 6, 23, 161, 582, 720, 1467, 1470, 2009
FspBI CTAG 7 cut(s) 267, 1097, 1556, 1589, 1599, 1640, 2004
GlaI GCGC 6 cut(s) 21, 90, 159, 647, 723, 1869
GluI GCNGC 8 cut(s) 6, 23, 161, 582, 720, 1467, 1470, 2009
GsuI CTGGAG 1 cut(s) 2124
HaeII RGCGCY 4 cut(s) 23, 92, 725, 1871
HaeIII GGCC 2 cut(s) 813, 1205
HapII CCGG 4 cut(s) 35, 452, 613, 627
HgaI GACGC 4 cut(s) 179, 180, 1124, 1706
HhaI GCGC 6 cut(s) 22, 91, 160, 648, 724, 1870
Hin1I GRCGYC 4 cut(s) 20, 171, 191, 1116
Hin6I GCGC 6 cut(s) 20, 89, 158, 646, 722, 1868
HinP1I GCGC 6 cut(s) 20, 89, 158, 646, 722, 1868
HincII GTYRAC 1 cut(s) 989
HindII GTYRAC 1 cut(s) 989
HpaII CCGG 4 cut(s) 35, 452, 613, 627
HphI GGTGA 4 cut(s) 851, 896, 1898, 1951
Hpy166II GTNNAC 4 cut(s) 989, 1784, 1847, 2227
Hpy8I GTNNAC 4 cut(s) 989, 1784, 1847, 2227
Hpy99I CGWCG 5 cut(s) 13, 173, 179, 187, 236
HpyAV CCTTC 3 cut(s) 533, 836, 1281
HpyCH4III ACNGT 6 cut(s) 130, 470, 999, 1508, 1842, 2039
HpyCH4IV ACGT 4 cut(s) 177, 231, 714, 1447
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 68, 101, 160, 730, 1568, 1867, 2207
HpySE526I ACGT 4 cut(s) 177, 231, 714, 1447
Hsp92I GRCGYC 4 cut(s) 20, 171, 191, 1116
HspAI GCGC 6 cut(s) 20, 89, 158, 646, 722, 1868
KasI GGCGCC 1 cut(s) 19
KpnI GGTACC 1 cut(s) 1148
Ksp22I TGATCA 2 cut(s) 1372, 1903
Kzo9I GATC 7 cut(s) 511, 1090, 1258, 1372, 1405, 1903, 2136
LmnI GCTCC 5 cut(s) 30, 101, 145, 448, 1180
Lsp1109I GCAGC 4 cut(s) 17, 147, 731, 1453
LweI GCATC 5 cut(s) 1483, 1580, 1801, 1883, 2020
MaeI CTAG 7 cut(s) 267, 1097, 1556, 1589, 1599, 1640, 2004
MaeII ACGT 4 cut(s) 177, 231, 714, 1447
MalI GATC 7 cut(s) 513, 1092, 1260, 1374, 1407, 1905, 2138
MbiI CCGCTC 2 cut(s) 104, 148
MboI GATC 7 cut(s) 511, 1090, 1258, 1372, 1405, 1903, 2136
MfeI CAATTG 1 cut(s) 2233
MflI RGATCY 2 cut(s) 1258, 1405
MhlI GDGCHC 4 cut(s) 35, 703, 1331, 1849
MlsI TGGCCA 2 cut(s) 813, 1205
MluNI TGGCCA 2 cut(s) 813, 1205
Mly113I GGCGCC 1 cut(s) 20
MlyI GAGTC 2 cut(s) 1778, 1797
MmeI TCCRAC 5 cut(s) 165, 186, 412, 660, 1815
Mox20I TGGCCA 2 cut(s) 813, 1205
MroXI GAANNNNTTC 2 cut(s) 567, 1218
MscI TGGCCA 2 cut(s) 813, 1205
MseI TTAA 9 cut(s) 372, 654, 759, 1526, 1542, 1577, 2114, 2172, 2239
MslI CAYNNNNRTG 3 cut(s) 706, 966, 1809
Msp20I TGGCCA 2 cut(s) 813, 1205
MspA1I CMGCKG 2 cut(s) 737, 1760
MspI CCGG 4 cut(s) 35, 452, 613, 627
MspR9I CCNGG 4 cut(s) 35, 404, 614, 1551
MunI CAATTG 1 cut(s) 2233
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 2 cut(s) 404, 1551
MvnI CGCG 3 cut(s) 158, 646, 648
MwoI GCNNNNNNNGC 8 cut(s) 11, 68, 101, 160, 730, 1568, 1867, 2207
NarI GGCGCC 1 cut(s) 20
NciI CCSGG 2 cut(s) 35, 614
NcoI CCATGG 1 cut(s) 1179
NdeII GATC 7 cut(s) 511, 1090, 1258, 1372, 1405, 1903, 2136
NlaIV GGNNCC 7 cut(s) 21, 32, 401, 450, 1146, 1185, 1260
NspV TTCGAA 2 cut(s) 227, 275
PagI TCATGA 2 cut(s) 961, 1084
PaqCI CACCTGC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 3 cut(s) 23, 174, 188
PctI GAATGC 1 cut(s) 607
PdmI GAANNNNTTC 2 cut(s) 567, 1218
PfeI GAWTC 8 cut(s) 111, 791, 905, 934, 1153, 1652, 1664, 2056
PkrI GCNGC 8 cut(s) 7, 24, 162, 583, 721, 1468, 1471, 2010
PleI GAGTC 2 cut(s) 1777, 1796
PluTI GGCGCC 1 cut(s) 23
PmaCI CACGTG 1 cut(s) 1448
PmlI CACGTG 1 cut(s) 1448
PpsI GAGTC 2 cut(s) 1777, 1796
Ppu21I YACGTR 1 cut(s) 1448
PshBI ATTAAT 2 cut(s) 372, 1542
PsiI TTATAA 2 cut(s) 1829, 2021
Psp124BI GAGCTC 1 cut(s) 1331
Psp6I CCWGG 2 cut(s) 402, 1549
PspCI CACGTG 1 cut(s) 1448
PspGI CCWGG 2 cut(s) 402, 1549
PspN4I GGNNCC 7 cut(s) 21, 32, 401, 450, 1146, 1185, 1260
PstI CTGCAG 1 cut(s) 1585
PstNI CAGNNNCTG 1 cut(s) 2197
PsuI RGATCY 2 cut(s) 1258, 1405
PvuII CAGCTG 1 cut(s) 1760
RsaI GTAC 3 cut(s) 1146, 1243, 2226
RsaNI GTAC 3 cut(s) 1145, 1242, 2225
RseI CAYNNNNRTG 3 cut(s) 706, 966, 1809
SacI GAGCTC 1 cut(s) 1331
SaqAI TTAA 9 cut(s) 372, 654, 759, 1526, 1542, 1577, 2114, 2172, 2239
SatI GCNGC 8 cut(s) 6, 23, 161, 582, 720, 1467, 1470, 2009
Sau3AI GATC 7 cut(s) 511, 1090, 1258, 1372, 1405, 1903, 2136
SchI GAGTC 2 cut(s) 1778, 1797
ScrFI CCNGG 4 cut(s) 35, 404, 614, 1551
SduI GDGCHC 4 cut(s) 35, 703, 1331, 1849
SfaNI GCATC 5 cut(s) 1483, 1580, 1801, 1883, 2020
SfcI CTRYAG 1 cut(s) 1581
SfoI GGCGCC 1 cut(s) 21
SfuI TTCGAA 2 cut(s) 227, 275
SmiMI CAYNNNNRTG 3 cut(s) 706, 966, 1809
SmlI CTYRAG 3 cut(s) 53, 506, 1739
SmoI CTYRAG 3 cut(s) 53, 506, 1739
SpeI ACTAGT 1 cut(s) 1598
SspDI GGCGCC 1 cut(s) 19
SspMI CTAG 7 cut(s) 267, 1097, 1556, 1589, 1599, 1640, 2004
SstI GAGCTC 1 cut(s) 1331
StyD4I CCNGG 4 cut(s) 33, 402, 612, 1549
StyI CCWWGG 3 cut(s) 243, 1179, 1389
TaaI ACNGT 6 cut(s) 130, 470, 999, 1508, 1842, 2039
TaiI ACGT 4 cut(s) 180, 234, 717, 1450
TatI WGTACW 1 cut(s) 1241
TauI GCSGC 4 cut(s) 25, 584, 1472, 2011
TfiI GAWTC 8 cut(s) 111, 791, 905, 934, 1153, 1652, 1664, 2056
Tru1I TTAA 9 cut(s) 372, 654, 759, 1526, 1542, 1577, 2114, 2172, 2239
Tru9I TTAA 9 cut(s) 372, 654, 759, 1526, 1542, 1577, 2114, 2172, 2239
TscAI CASTG 2 cut(s) 475, 1847
TseI GCWGC 4 cut(s) 5, 160, 719, 1466
TspGWI ACGGA 1 cut(s) 295
TspRI CASTG 2 cut(s) 475, 1847
VneI GTGCAC 1 cut(s) 1845
VspI ATTAAT 2 cut(s) 372, 1542
XapI RAATTY 4 cut(s) 122, 209, 673, 980
XcmI CCANNNNNNNNNTGG 1 cut(s) 1177
XmiI GTMKAC 1 cut(s) 1783
XmnI GAANNNNTTC 2 cut(s) 567, 1218
XspI CTAG 7 cut(s) 267, 1097, 1556, 1589, 1599, 1640, 2004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.