MD02G1136400.v1.1

N-terminal region of micro-spherule protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010517
Physical Location & Seq
Reverse (-)
11377221 .. 11382050
4830 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1136400.v1.1.491

Sequence Viewer

Length: 2499 bp
ATGGAAGCAGTGGCTTCGGCTCCTCCATGGATGCCGGAAGACGACCTCCGCTTGAAGGATGCCATGGAGACAGGTGCTTCTCTGGAAGCACTTGCCAGAGGCGCGGTGCGATTTTCGCGCAAATTCACGATCGGAGAGTTGCGGGAGCGGTGGTGCTCGCTCCTCTACGACGCTGATGTCTCGGCGGAGGCGTCGGCTCGGTTGGTGAAGTTGGAAGGTTGCAATTCCAACTCAGCGTCCAAGGCTAGTAGATTCAGTCGTTCTAGGGGTTTGCAGCGAAAATCCGATAGCATACGAAAGCATTACTATGCTATGCAGAAGAGACTGGGCGATGTTAATTCCGTTGATCCCGATTCTTATCAGAAGGAGTTTTTCGATCGGAATTTTCTTTTCAGGCCGGACATTGATAATGGCGAGGCTTTTGAGGAAAATTTTGGGGCTGGAGATCATAATCAGCCCTTGTTTTTGGATTGTGATGTGGATAACGGCGATGCTGCTAATGCATTTCATGGTGGAGGGCGTGTTTCGATTGGAAATCATGGTGCGGAGGGAGGTGGAGAGCGTGTTTCGATTGGAAATCAAGGTGTGGAGGGAGGTGGGGAGTGTGTTCCAATTGGAAATCATGTTGTGGAGGGATTTGTGGGGGAAGGGTGCTCAAATGAATTTGTCGAACAGGTTTCTTTGCTGCCAGGTAACGAATTAGGAGAGAATGCTTTTTGCCGTGATAATGCCTGTCAAGATCTGCCTTCTAATCGAGATGATGCAATTGATTTTGGGAATGCCTTAGATGCGGAAGATATAGGGCCATCTCATGCATCTATAGATGAGCCTCTTTGGAAAACGATTGAGGATGTACGGGCACCAGAAATGCCAATGGATGTCAGCATGGGTGTTAATGGTGAGGATGCCAAAAAGACATTGGTAGTTACTGATGATATGGATGTGGATAATATTGGTTCATCTCAATATGACGATATCCATTCTGAGGAAGTGTTTAATGATGAACTGATTAGGTCTGTCACAGTTTCTGGAGGTGATTATGCAGACATAGACCTTGCGAATGAGTATGAGCTTGCCTTCATGTATGCAAGTGGCAAGGAGTCAATAGATAAGTCCTCTTCTGAAAACCTGAATCCGATTTCGTTGAGTAAGACCAAAGATGTTCATGAAAATGATGTACCGTCTCACTCTGGCCAAGATCTTCCCCCAAAGTTGGTTTCAGATTCCTTCCAGGTTGTTACAGACAATATGCAGGCTGCCGAGATGGATGTTGCTGCTGAACTGTCTCACTCTGGCCAAGATGATCAGCGTGTTATCTCTTGTTCCGAAGCCAATATGACAGCATCTACGTCAGTGCCACATCCTCTTACTCCTGAACGTAATCATGAAGAGATGATTTGCACATTGAATACCGAGGACCCTGAAATCCCATGCAATGATGATATATTTCCATCTACTGCAACTGTTCATGCTGTAGGGGAACCAACCTTAAAAGGAGCTCATGAGTTGGCTTCCTCAACTGGTAAAAGAAAATGCGATCAACAAAGGAAAGAAGAAGATCCTGCACGACCTTTTAAGGTTCCCCGGATGGTGGGATACGACACAAGTGCAGAAAACAGCCCTAACCATGCACTTGGTTCTTTTGGAGTTAAAGCTCCATATGGTGATAGTAAATGTGTAGCTTCAGTCTCCAAACATGATAAAAATGTCATTGCCGACCAAAGCCAAAGCAGATCAGCACATGCACCATCTAAGTCTATCACCAATCAAGGGCTCAAAGAAGAGGGACTAGAAGCTCCTTTTACAATTGCAGAACTTGCACCCTTATGCGCAGAGGGTTCTACGACTTTCCCAGAACCAGAAGCCAACCCATCAGTACTAGATCGTGAAGAATCTGAGGAAGAATATGATGATGATGCTGACATTCCTTGTTATTCTGATATTGAAGCGATGATACTTGAGATGGATTTATATCCACTGGATCAGGATTCGTACATCAGTAGTGAAGTCTTAGAGTATCAAAACGAGGATTCTAAAAGAAAAATCATGAGGTTGGAACAGTGTGCACGATCCTCTATGCAAAGAACCCTTGCATCTAAAGGTGCACTTGCAGTATTGTATGGAAACCAGATAAAGGAATACATTATGAAAACTGAGGTAATAATTGGCAGATCAACAGAAGATAACGAAGTTGATATTGACTTGGGAAAGGAAGGGCGGCATAAGAAAATATCTCGGCGGCAGGCTCTTATAAAGATGGAAGGAGATGGTTCTTTCTCTTTGAAGAATCTTGGCAAGAGTTCAATATTTTTGAATGGTGAAGAAGTTGCTTCGGGACAGCGTGTTAGTCTTAGTTCAAGTAATTTGATTGAGATTAGGGACATGTATTTTAATTTAGAGACGAATCACAAATCTGCGAGGCAGTATCTGGGAAGAATTGGCCAGCAAAGCGAGGATAAGCACACCAAGTTCGAATGGTCACCTGAGAGAGGCCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0002151 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0004857 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005844 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010521 GO:0010556 GO:0010558 GO:0010605 GO:0010639 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0016579 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030234 GO:0030425 GO:0031011 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033202 GO:0034046 GO:0034708 GO:0035097 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043025 GO:0043086 GO:0043170 GO:0043204 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044463 GO:0044464 GO:0044665 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051338 GO:0051348 GO:0051972 GO:0051974 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070603 GO:0070646 GO:0070647 GO:0070717 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097346 GO:0097447 GO:0097458 GO:0098772 GO:0120025 GO:0120038 GO:1900180 GO:1900182 GO:1901363 GO:1901564 GO:1902493 GO:1902494 GO:1903827 GO:1903829 GO:1904356 GO:1904357 GO:1904749 GO:1904751 GO:1904949 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
FHA MCRS_N

Protein Analysis

833

Amino Acids

91.26

Weight (kDa)

4.6

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCRS_N PF13325 10 - 87 2.1e-13 N-terminal region of micro-spherule protein
FHA PF00498 720 - 792 2.6e-10 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2252
AasI GACNNNNNNGTC 1 cut(s) 176
Acc16I TGCGCA 1 cut(s) 1831
AccB1I GGYRCC 1 cut(s) 859
AccBSI CCGCTC 1 cut(s) 148
AccII CGCG 2 cut(s) 104, 118
AciI CCGC 9 cut(s) 49, 104, 142, 148, 185, 545, 791, 2218, 2239
AclWI GGATC 4 cut(s) 341, 1553, 1989, 2064
AcoI YGGCCR 3 cut(s) 1192, 1294, 2440
AcsI RAATTY 4 cut(s) 122, 382, 430, 662
AcuI CTGAAG 1 cut(s) 1668
AcyI GRCGYC 1 cut(s) 191
AfaI GTAC 4 cut(s) 855, 1179, 1878, 1994
AfiI CCNNNNNNNGG 7 cut(s) 55, 985, 1213, 1591, 1770, 2134, 2489
AflIII ACRYGT 1 cut(s) 2382
AgsI TTSAA 7 cut(s) 55, 1408, 1946, 2284, 2304, 2314, 2358
AjnI CCWGG 2 cut(s) 688, 1230
AjuI GAANNNNNNNTTGG 2 cut(s) 1201, 1233
AluBI AGCT 5 cut(s) 1072, 1499, 1655, 1682, 1796
AluI AGCT 5 cut(s) 1072, 1499, 1655, 1682, 1796
Alw21I GWGCWC 5 cut(s) 158, 656, 1501, 2068, 2107
Alw26I GTCTC 7 cut(s) 62, 184, 316, 1188, 1290, 1693, 2393
Alw44I GTGCAC 2 cut(s) 2064, 2103
AlwI GGATC 4 cut(s) 341, 1553, 1989, 2064
AlwNI CAGNNNCTG 2 cut(s) 1028, 2428
AoxI GGCC 6 cut(s) 395, 803, 1192, 1294, 2440, 2491
ApaLI GTGCAC 2 cut(s) 2064, 2103
ApeKI GCWGC 5 cut(s) 274, 494, 685, 1256, 1274
ApoI RAATTY 4 cut(s) 122, 382, 430, 662
AspLEI GCGC 3 cut(s) 104, 120, 1832
AspS9I GGNCC 3 cut(s) 803, 1417, 2492
AsuC2I CCSGG 1 cut(s) 1585
AsuHPI GGTGA 7 cut(s) 217, 911, 1046, 1676, 1753, 2330, 2472
AsuII TTCGAA 1 cut(s) 2472
AvaII GGWCC 1 cut(s) 1417
BaeGI GKGCMC 3 cut(s) 862, 2068, 2107
BalI TGGCCA 3 cut(s) 1194, 1296, 2442
BanI GGYRCC 1 cut(s) 859
BanII GRGCYC 2 cut(s) 1501, 1776
BarI GAAGNNNNNNTAC 2 cut(s) 1938, 1970
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 5 cut(s) 158, 656, 1501, 2068, 2107
BbvI GCAGC 5 cut(s) 286, 481, 672, 1243, 1261
BccI CCATC 9 cut(s) 814, 1258, 1459, 1582, 1756, 1879, 1957, 2251, 2261
BceAI ACGGC 2 cut(s) 502, 705
BcgI CGANNNNNNTGC 3 cut(s) 31, 1589, 1623
BciT130I CCWGG 2 cut(s) 690, 1232
BciVI GTATCC 1 cut(s) 1589
BclI TGATCA 1 cut(s) 1303
BcnI CCSGG 1 cut(s) 1585
BcoDI GTCTC 7 cut(s) 62, 184, 316, 1188, 1290, 1693, 2393
BfaI CTAG 4 cut(s) 246, 264, 1790, 1880
BfmI CTRYAG 2 cut(s) 819, 1473
BfuI GTATCC 1 cut(s) 1589
BglII AGATCT 2 cut(s) 739, 1198
BisI GCNGC 7 cut(s) 275, 495, 686, 1257, 1275, 2219, 2240
BlsI GCNGC 7 cut(s) 276, 496, 687, 1258, 1276, 2220, 2241
BmcAI AGTACT 1 cut(s) 1878
Bme1390I CCNGG 3 cut(s) 690, 1232, 1585
Bme18I GGWCC 1 cut(s) 1417
BmgT120I GGNCC 3 cut(s) 803, 1417, 2492
BmiI GGNNCC 5 cut(s) 21, 861, 1419, 1482, 1581
BmrFI CCNGG 3 cut(s) 690, 1232, 1585
BmrI ACTGGG 1 cut(s) 335
BmuI ACTGGG 1 cut(s) 335
BpiI GAAGAC 1 cut(s) 45
BpmI CTGGAG 2 cut(s) 462, 1050
Bpu14I TTCGAA 1 cut(s) 2472
BpuEI CTTGAG 1 cut(s) 1979
BpuMI CCSGG 1 cut(s) 1585
BsaBI GATNNNNATC 3 cut(s) 357, 450, 1971
BsaHI GRCGYC 1 cut(s) 191
BsaJI CCNNGG 5 cut(s) 26, 63, 240, 1413, 1583
BsaXI ACNNNNNCTCC 6 cut(s) 137, 167, 507, 537, 549, 579
Bsc4I CCNNNNNNNGG 7 cut(s) 55, 985, 1213, 1591, 1770, 2134, 2489
Bse1I ACTGG 3 cut(s) 330, 1525, 1983
Bse3DI GCAATG 2 cut(s) 1441, 1710
Bse8I GATNNNNATC 3 cut(s) 357, 450, 1971
BseBI CCWGG 2 cut(s) 690, 1232
BseDI CCNNGG 5 cut(s) 26, 63, 240, 1413, 1583
BseGI GGATG 9 cut(s) 36, 64, 856, 883, 910, 946, 1273, 1360, 1593
BseJI GATNNNNATC 3 cut(s) 357, 450, 1971
BseLI CCNNNNNNNGG 7 cut(s) 55, 985, 1213, 1591, 1770, 2134, 2489
BseMI GCAATG 2 cut(s) 1441, 1710
BseMII CTCAG 5 cut(s) 246, 975, 1887, 2145, 2475
BseNI ACTGG 3 cut(s) 330, 1525, 1983
BseRI GAGGAG 2 cut(s) 12, 152
BseSI GKGCMC 3 cut(s) 862, 2068, 2107
BseXI GCAGC 5 cut(s) 286, 481, 672, 1243, 1261
BsgI GTGCAG 2 cut(s) 1548, 1629
Bsh1236I CGCG 2 cut(s) 104, 118
Bsh1285I CGRYCG 2 cut(s) 132, 379
BshFI GGCC 6 cut(s) 397, 805, 1194, 1296, 2442, 2493
BshNI GGYRCC 1 cut(s) 859
BsiEI CGRYCG 2 cut(s) 132, 379
BsiHKAI GWGCWC 5 cut(s) 158, 656, 1501, 2068, 2107
BsiSI CCGG 3 cut(s) 35, 398, 1585
BslFI GGGAC 3 cut(s) 1800, 2349, 2393
BslI CCNNNNNNNGG 7 cut(s) 55, 985, 1213, 1591, 1770, 2134, 2489
BsmAI GTCTC 7 cut(s) 62, 184, 316, 1188, 1290, 1693, 2393
BsmBI CGTCTC 2 cut(s) 1188, 2393
BsmFI GGGAC 3 cut(s) 1800, 2349, 2393
BsmI GAATGC 2 cut(s) 715, 784
BsnI GGCC 6 cut(s) 397, 805, 1194, 1296, 2442, 2493
Bsp119I TTCGAA 1 cut(s) 2472
Bsp1286I GDGCHC 7 cut(s) 158, 656, 862, 1501, 1776, 2068, 2107
Bsp19I CCATGG 2 cut(s) 26, 63
BspACI CCGC 9 cut(s) 49, 104, 142, 148, 185, 545, 791, 2218, 2239
BspANI GGCC 6 cut(s) 397, 805, 1194, 1296, 2442, 2493
BspCNI CTCAG 5 cut(s) 245, 976, 1888, 2146, 2476
BspFNI CGCG 2 cut(s) 104, 118
BspHI TCATGA 4 cut(s) 1165, 1384, 1501, 2046
BspLI GGNNCC 5 cut(s) 21, 861, 1419, 1482, 1581
BspPI GGATC 4 cut(s) 341, 1553, 1989, 2064
BspT104I TTCGAA 1 cut(s) 2472
BspT107I GGYRCC 1 cut(s) 859
BsrBI CCGCTC 1 cut(s) 148
BsrDI GCAATG 2 cut(s) 1441, 1710
BsrI ACTGG 3 cut(s) 330, 1525, 1983
BssECI CCNNGG 5 cut(s) 26, 63, 240, 1413, 1583
BssNI GRCGYC 1 cut(s) 191
BssT1I CCWWGG 3 cut(s) 26, 63, 240
Bst2UI CCWGG 2 cut(s) 690, 1232
Bst4CI ACNGT 5 cut(s) 1024, 1182, 1284, 1465, 2061
Bst6I CTCTTC 4 cut(s) 314, 1123, 1383, 1776
BstACI GRCGYC 1 cut(s) 191
BstAPI GCANNNNNTGC 1 cut(s) 1817
BstBI TTCGAA 1 cut(s) 2472
BstC8I GCNNGC 5 cut(s) 158, 1074, 1254, 2244, 2444
BstDEI CTNAG 9 cut(s) 232, 784, 984, 1752, 1896, 2011, 2154, 2351, 2484
BstDSI CCRYGG 2 cut(s) 26, 63
BstEII GGTNACC 1 cut(s) 2478
BstENI CCTNNNNNAGG 1 cut(s) 2487
BstF5I GGATG 9 cut(s) 36, 64, 856, 883, 910, 946, 1273, 1360, 1593
BstFNI CGCG 2 cut(s) 104, 118
BstHHI GCGC 3 cut(s) 104, 120, 1832
BstMAI GTCTC 7 cut(s) 62, 184, 316, 1188, 1290, 1693, 2393
BstMCI CGRYCG 2 cut(s) 132, 379
BstMWI GCNNNNNNNGC 7 cut(s) 101, 115, 242, 500, 788, 1817, 2448
BstNI CCWGG 2 cut(s) 690, 1232
BstNSI RCATGY 2 cut(s) 1745, 2386
BstPI GGTNACC 1 cut(s) 2478
BstSCI CCNGG 3 cut(s) 688, 1230, 1583
BstSFI CTRYAG 2 cut(s) 819, 1473
BstSLI GKGCMC 3 cut(s) 862, 2068, 2107
BstUI CGCG 2 cut(s) 104, 118
BstV1I GCAGC 5 cut(s) 286, 481, 672, 1243, 1261
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 3 cut(s) 739, 1198, 1558
BstXI CCANNNNNNTGG 1 cut(s) 1634
BstYI RGATCY 3 cut(s) 739, 1198, 1558
BsuI GTATCC 1 cut(s) 1589
BsuRI GGCC 6 cut(s) 397, 805, 1194, 1296, 2442, 2493
BtgI CCRYGG 2 cut(s) 26, 63
BtgZI GCGATG 3 cut(s) 345, 504, 1964
BtsCI GGATG 9 cut(s) 36, 64, 856, 883, 910, 946, 1273, 1360, 1593
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 4 cut(s) 15, 1359, 1976, 2066
Cac8I GCNNGC 5 cut(s) 158, 1074, 1254, 2244, 2444
CaiI CAGNNNCTG 2 cut(s) 1028, 2428
CciI TCATGA 4 cut(s) 1165, 1384, 1501, 2046
CfoI GCGC 3 cut(s) 104, 120, 1832
Cfr13I GGNCC 3 cut(s) 803, 1417, 2492
CseI GACGC 3 cut(s) 179, 180, 225
Csp6I GTAC 4 cut(s) 854, 1178, 1877, 1993
CviQI GTAC 4 cut(s) 854, 1178, 1877, 1993
DdeI CTNAG 9 cut(s) 232, 784, 984, 1752, 1896, 2011, 2154, 2351, 2484
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
EaeI YGGCCR 3 cut(s) 1192, 1294, 2440
Eam1104I CTCTTC 4 cut(s) 314, 1123, 1383, 1776
EarI CTCTTC 4 cut(s) 314, 1123, 1383, 1776
EciI GGCGGA 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 1499
Eco130I CCWWGG 3 cut(s) 26, 63, 240
Eco24I GRGCYC 2 cut(s) 1501, 1776
Eco32I GATATC 1 cut(s) 976
Eco47I GGWCC 1 cut(s) 1417
Eco53kI GAGCTC 1 cut(s) 1499
Eco57I CTGAAG 1 cut(s) 1668
Eco91I GGTNACC 1 cut(s) 2478
EcoICRI GAGCTC 1 cut(s) 1499
EcoNI CCTNNNNNAGG 1 cut(s) 2487
EcoO109I RGGNCCY 1 cut(s) 1417
EcoO65I GGTNACC 1 cut(s) 2478
EcoRII CCWGG 2 cut(s) 688, 1230
EcoRV GATATC 1 cut(s) 976
EcoT14I CCWWGG 3 cut(s) 26, 63, 240
EcoT22I ATGCAT 2 cut(s) 505, 817
EcoT38I GRGCYC 2 cut(s) 1501, 1776
ErhI CCWWGG 3 cut(s) 26, 63, 240
Esp3I CGTCTC 2 cut(s) 1188, 2393
FalI AAGNNNNNCTT 2 cut(s) 2091, 2123
FaqI GGGAC 3 cut(s) 1800, 2349, 2393
FauI CCCGC 1 cut(s) 135
FauNDI CATATG 1 cut(s) 1660
FbaI TGATCA 1 cut(s) 1303
Fnu4HI GCNGC 7 cut(s) 275, 495, 686, 1257, 1275, 2219, 2240
FokI GGATG 9 cut(s) 43, 71, 863, 890, 917, 953, 1280, 1347, 1600
FriOI GRGCYC 2 cut(s) 1501, 1776
Fsp4HI GCNGC 7 cut(s) 275, 495, 686, 1257, 1275, 2219, 2240
FspBI CTAG 4 cut(s) 246, 264, 1790, 1880
FspI TGCGCA 1 cut(s) 1831
GlaI GCGC 3 cut(s) 103, 119, 1831
GluI GCNGC 7 cut(s) 275, 495, 686, 1257, 1275, 2219, 2240
GsuI CTGGAG 2 cut(s) 462, 1050
HaeIII GGCC 6 cut(s) 397, 805, 1194, 1296, 2442, 2493
HapII CCGG 3 cut(s) 35, 398, 1585
HgaI GACGC 3 cut(s) 179, 180, 225
HhaI GCGC 3 cut(s) 104, 120, 1832
Hin1I GRCGYC 1 cut(s) 191
Hin6I GCGC 3 cut(s) 102, 118, 1830
HinP1I GCGC 3 cut(s) 102, 118, 1830
HpaII CCGG 3 cut(s) 35, 398, 1585
HphI GGTGA 7 cut(s) 217, 911, 1046, 1676, 1753, 2330, 2472
Hpy166II GTNNAC 2 cut(s) 2066, 2105
Hpy8I GTNNAC 2 cut(s) 2066, 2105
Hpy99I CGWCG 2 cut(s) 173, 196
HpyAV CCTTC 9 cut(s) 49, 209, 358, 641, 756, 1087, 1237, 2207, 2255
HpyCH4III ACNGT 5 cut(s) 1024, 1182, 1284, 1465, 2061
HpyCH4IV ACGT 2 cut(s) 1349, 1378
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 115, 242, 500, 788, 1817, 2448
HpyF3I CTNAG 9 cut(s) 232, 784, 984, 1752, 1896, 2011, 2154, 2351, 2484
HpySE526I ACGT 2 cut(s) 1349, 1378
Hsp92I GRCGYC 1 cut(s) 191
HspAI GCGC 3 cut(s) 102, 118, 1830
Ksp22I TGATCA 1 cut(s) 1303
LmnI GCTCC 6 cut(s) 25, 145, 165, 1496, 1660, 1801
Lsp1109I GCAGC 5 cut(s) 286, 481, 672, 1243, 1261
MaeI CTAG 4 cut(s) 246, 264, 1790, 1880
MaeII ACGT 2 cut(s) 1349, 1378
MaeIII GTNAC 5 cut(s) 692, 925, 1018, 1237, 2478
MbiI CCGCTC 1 cut(s) 148
MfeI CAATTG 3 cut(s) 612, 765, 1806
MflI RGATCY 3 cut(s) 739, 1198, 1558
MhlI GDGCHC 7 cut(s) 158, 656, 862, 1501, 1776, 2068, 2107
MlsI TGGCCA 3 cut(s) 1194, 1296, 2442
MluNI TGGCCA 3 cut(s) 1194, 1296, 2442
MlyI GAGTC 1 cut(s) 1109
MmeI TCCRAC 3 cut(s) 192, 252, 2034
Mox20I TGGCCA 3 cut(s) 1194, 1296, 2442
Mph1103I ATGCAT 2 cut(s) 505, 817
MscI TGGCCA 3 cut(s) 1194, 1296, 2442
MseI TTAA 7 cut(s) 336, 894, 996, 1490, 1575, 1650, 2391
MslI CAYNNNNRTG 3 cut(s) 306, 1170, 1825
Msp20I TGGCCA 3 cut(s) 1194, 1296, 2442
MspI CCGG 3 cut(s) 35, 398, 1585
MspR9I CCNGG 3 cut(s) 690, 1232, 1585
MunI CAATTG 3 cut(s) 612, 765, 1806
Mva1269I GAATGC 2 cut(s) 715, 784
MvaI CCWGG 2 cut(s) 690, 1232
MvnI CGCG 2 cut(s) 104, 118
MwoI GCNNNNNNNGC 7 cut(s) 101, 115, 242, 500, 788, 1817, 2448
NciI CCSGG 1 cut(s) 1585
NcoI CCATGG 2 cut(s) 26, 63
NdeI CATATG 1 cut(s) 1660
NlaIV GGNNCC 5 cut(s) 21, 861, 1419, 1482, 1581
NmeAIII GCCGAG 3 cut(s) 161, 1285, 2215
NmuCI GTSAC 2 cut(s) 1018, 2478
NsbI TGCGCA 1 cut(s) 1831
NsiI ATGCAT 2 cut(s) 505, 817
NspI RCATGY 2 cut(s) 1745, 2386
NspV TTCGAA 1 cut(s) 2472
PagI TCATGA 4 cut(s) 1165, 1384, 1501, 2046
PciI ACATGT 1 cut(s) 2382
PcsI WCGNNNNNNNCGW 1 cut(s) 188
PctI GAATGC 2 cut(s) 715, 784
PfeI GAWTC 9 cut(s) 252, 353, 1132, 1223, 1892, 1988, 2030, 2287, 2404
PkrI GCNGC 7 cut(s) 276, 496, 687, 1258, 1276, 2220, 2241
Ple19I CGATCG 2 cut(s) 132, 379
PleI GAGTC 1 cut(s) 1108
PpsI GAGTC 1 cut(s) 1108
PpuMI RGGWCCY 1 cut(s) 1417
PscI ACATGT 1 cut(s) 2382
PsiI TTATAA 1 cut(s) 2252
Psp124BI GAGCTC 1 cut(s) 1501
Psp5II RGGWCCY 1 cut(s) 1417
Psp6I CCWGG 2 cut(s) 688, 1230
PspEI GGTNACC 1 cut(s) 2478
PspGI CCWGG 2 cut(s) 688, 1230
PspN4I GGNNCC 5 cut(s) 21, 861, 1419, 1482, 1581
PspPI GGNCC 3 cut(s) 803, 1417, 2492
PspPPI RGGWCCY 1 cut(s) 1417
PstNI CAGNNNCTG 2 cut(s) 1028, 2428
PsuI RGATCY 3 cut(s) 739, 1198, 1558
PvuI CGATCG 2 cut(s) 132, 379
RsaI GTAC 4 cut(s) 855, 1179, 1878, 1994
RsaNI GTAC 4 cut(s) 854, 1178, 1877, 1993
RseI CAYNNNNRTG 3 cut(s) 306, 1170, 1825
SacI GAGCTC 1 cut(s) 1501
SaqAI TTAA 7 cut(s) 336, 894, 996, 1490, 1575, 1650, 2391
SatI GCNGC 7 cut(s) 275, 495, 686, 1257, 1275, 2219, 2240
Sau96I GGNCC 3 cut(s) 803, 1417, 2492
ScaI AGTACT 1 cut(s) 1878
SchI GAGTC 1 cut(s) 1109
ScrFI CCNGG 3 cut(s) 690, 1232, 1585
SduI GDGCHC 7 cut(s) 158, 656, 862, 1501, 1776, 2068, 2107
SfcI CTRYAG 2 cut(s) 819, 1473
SfuI TTCGAA 1 cut(s) 2472
SinI GGWCC 1 cut(s) 1417
SmiMI CAYNNNNRTG 3 cut(s) 306, 1170, 1825
SmlI CTYRAG 1 cut(s) 1958
SmoI CTYRAG 1 cut(s) 1958
SsiI CCGC 9 cut(s) 49, 104, 142, 148, 185, 545, 791, 2218, 2239
SspI AATATT 2 cut(s) 952, 2307
SspMI CTAG 4 cut(s) 246, 264, 1790, 1880
SstI GAGCTC 1 cut(s) 1501
StyD4I CCNGG 3 cut(s) 688, 1230, 1583
StyI CCWWGG 3 cut(s) 26, 63, 240
TaaI ACNGT 5 cut(s) 1024, 1182, 1284, 1465, 2061
TaiI ACGT 2 cut(s) 1352, 1381
TaqI TCGA 6 cut(s) 375, 527, 569, 669, 754, 2472
TatI WGTACW 1 cut(s) 1876
TauI GCSGC 2 cut(s) 2221, 2242
TfiI GAWTC 9 cut(s) 252, 353, 1132, 1223, 1892, 1988, 2030, 2287, 2404
Tru1I TTAA 7 cut(s) 336, 894, 996, 1490, 1575, 1650, 2391
Tru9I TTAA 7 cut(s) 336, 894, 996, 1490, 1575, 1650, 2391
TscAI CASTG 4 cut(s) 15, 1359, 1983, 2066
TseFI GTSAC 2 cut(s) 1018, 2478
TseI GCWGC 5 cut(s) 274, 494, 685, 1256, 1274
Tsp45I GTSAC 2 cut(s) 1018, 2478
TspGWI ACGGA 1 cut(s) 331
TspRI CASTG 4 cut(s) 15, 1359, 1983, 2066
VneI GTGCAC 2 cut(s) 2064, 2103
VpaK11BI GGWCC 1 cut(s) 1417
XagI CCTNNNNNAGG 1 cut(s) 2487
XapI RAATTY 4 cut(s) 122, 382, 430, 662
XceI RCATGY 2 cut(s) 1745, 2386
XcmI CCANNNNNNNNNTGG 1 cut(s) 916
XspI CTAG 4 cut(s) 246, 264, 1790, 1880
ZrmI AGTACT 1 cut(s) 1878
Zsp2I ATGCAT 2 cut(s) 505, 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.