Rroxscaffold_2G00142610

N-terminal region of micro-spherule protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010517
Physical Location & Seq
Forward (+)
80719406 .. 80723433
4028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00142610.1

Sequence Viewer

Length: 2259 bp
ATGGCAGCGACGGCTTCGGCGCCGCCGTGGATCCCGGAAGACGACCTCCTCCTCAAGAACGCCATCGAGGCAGGTGCTTCTCTGGAAGCGCTTGCCAGAGGAGCGGTCAGATTCTCCCGCAAATTCACGGTGGGAGATTTGCGGGAGCGGTGGCACGCGCTGCTCTACGACGCCGACGTGTCGTCGGAGGCGTCTGCTCGGATGTTGGAATTCGAGGCGTGTAATTCGAACGTCGTGCCGTCCAAGGCTAATAGTGATTTCGATATTAATTTTCTTGGTGGGCAAGCTCTTGGGAACCAGGGGAGTTTGGATGGGAATTGTATGGTTGGAGGTCATGTTGGGGAGCCGGTTTTCAATGGCGCCGGAGGGAGTGTTTTTCATGGCGGAGAGTGTGTCTCTCTTGAGGATCATGGGGTAGGCGGAGTTGGGAGGGAAGGTTGTGGTAATGAGTTTTCGGAACAGGTTCTTTTAGTGGCGGCGAATGGATTGGGAGAGAATGCTTATTGCCGGGATAATAATGCCGCTGAGGATTTATCTACGCGCGGGGTTAATTCAATGGATTTTGGGAATTCGTTGGATGCGGAAGATATAGGGGCACATTTATGGGACGTAGCAGCGCCAGAAATGCCAGCGGAAGAGACATTGGTAGGTGTTAATGATGTGGATGTCGATGGGGAGAATATGGATTCATCTCAATATGAAGTTGGCCATACTGAGGCAATGTTGGTTGATGGTGAAGGGTGTGATGAACTGAATAGGCGTGTAGCTATATCGGGAGGTGATTATGGAGATATGGCAGATTCTCTTTTCAATGATGAGGTCAATTCGATTCTGTTGAGTTCTCCCAAAGATGTTCATGAAGATGATGTTCCTGAGTTCTGTCAACCTCCACAGTTGGTTTCAGTTTCATGTGAGGCAGTTTCAGATGTTGTGCATACTGCTGAGATGGTTCTTTCTGTCGAACCATCTCAGACTGGTCATGATGATCGGCTAGATATTTCATGTTCAGACGCCAATAAGCCATCATCATCCACATCAGTACCATTGAATCCTACCTTGATGACTGTTGCTCCCATGGTGCCTGTTGCTCCTGAGATGGCCGTTGCAGAAGAGGTTCAAGAAGAAGAGATGATTTGTACATTGAATACTGAGGATCCTGACATCCCTTCCAATGATGATATCATGTTTCCTACTGCGATTGTTCATACTTCCCTGCAACCGAGCTCTAAAGAAGTTAGTAATTTGGCTACTGACCAAAGAAAAAGTGATCACCAAATAAAAACCTTGGAGAAAAAAGAAGATCCTGCACAACCTTTTAGAAAAGAAAGTCCAAACCCTGCACGTGATAAAAATGACCCTGCTGCCGCAAGCCAATGCAGATTAGCAGATGCAACTCTCAACTGTATCACAAAGGGATTGCTAAATGAAGAGGTTATGATTTTTTACTTGGGATCACTTCATACTGTTGATATATCATACATTGGTTCTTCAACTTATGAATTTCTTTTGGAAATATCTATCACTAAATCTGATTGTTGCAAACAGGAAATCAATGTTCCAGGTATAGTTGCAGAACTTGCATCTTTAACTGCAGAGCTAGGTTCTACTAGTATGGCTTTCCCAGAACCACAAGCCAACCCCTCAGCACTAGATTGTGAAGAATCTGAGGAAGAATCTAATGACGATGTTGATGATGATGACGAGGACGCTGACATTCCTTATTTCTCTGATATCGAAACGATGATACTTGAGATGGATTTATGTCCAGCTGATGAGGAGTCAGATATCAGTAGACGAGTCTCAAAGTATCAACATGAGGATGCTAAAAGGAAAATTGTAAGGTTGGAACAGTGTGCACAATCCTCTATGCAAAGAGCGCTTGCATCTAAAGGCACACTTGCTGTATTGTATGATCACCATGTAAAGCATTATATCAAGAAAACTGAGGTGATAATTGGCAGAACAACAGAAGATAATCACGTTGATATTGATTTGGGAGATAACAAAGTATCTAGGCGGCAGGCTCTTATAAAGATGGATGCAGATGGTTCTTTCTCATTGAAGAATCTTGGCAAGAGTTCAATTTTCTTGAATGGCAGAGAAATTGCTACCGGAGAGCTTTTAAGTTTTGCTTCGAGTAATTTGATTGAGATTAGGGAGATGGCATTTGTTTTTGAGATTAACCACAAATCTGTGAGGCAGTATCTGGCAAAAATTGCCACAAAAAGTGAGAGGAGCATCCTCACTCTGCCCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0002151 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0004857 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005844 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010521 GO:0010556 GO:0010558 GO:0010605 GO:0010639 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0016579 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030234 GO:0030425 GO:0031011 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033202 GO:0034046 GO:0034708 GO:0035097 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043025 GO:0043086 GO:0043170 GO:0043204 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044463 GO:0044464 GO:0044665 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051338 GO:0051348 GO:0051972 GO:0051974 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070603 GO:0070646 GO:0070647 GO:0070717 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097346 GO:0097447 GO:0097458 GO:0098772 GO:0120025 GO:0120038 GO:1900180 GO:1900182 GO:1901363 GO:1901564 GO:1902493 GO:1902494 GO:1903827 GO:1903829 GO:1904356 GO:1904357 GO:1904749 GO:1904751 GO:1904949 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
FHA MCRS_N

Protein Analysis

752

Amino Acids

81.46

Weight (kDa)

4.35

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCRS_N PF13325 10 - 79 2.4e-13 N-terminal region of micro-spherule protein
FHA PF00498 650 - 704 2.4e-08 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2030
AarI CACCTGC 1 cut(s) 62
Acc36I ACCTGC 1 cut(s) 62
AccB1I GGYRCC 3 cut(s) 19, 359, 1078
AccBSI CCGCTC 2 cut(s) 104, 148
AccI GTMKAC 1 cut(s) 1792
AccII CGCG 3 cut(s) 158, 541, 543
AclWI GGATC 7 cut(s) 25, 38, 414, 1148, 1161, 1295, 1459
AcoI YGGCCR 2 cut(s) 706, 1098
AcsI RAATTY 4 cut(s) 122, 209, 568, 1499
AcvI CACGTG 1 cut(s) 1343
AcyI GRCGYC 5 cut(s) 20, 171, 191, 360, 1011
AfaI GTAC 2 cut(s) 1041, 1138
AfeI AGCGCT 2 cut(s) 90, 1878
AfiI CCNNNNNNNGG 1 cut(s) 715
AflIII ACRYGT 1 cut(s) 177
AhdI GACNNNNNGTC 1 cut(s) 181
AhlI ACTAGT 1 cut(s) 1607
AjiI CACGTC 1 cut(s) 178
AjnI CCWGG 2 cut(s) 297, 1558
AluBI AGCT 6 cut(s) 287, 767, 1224, 1597, 1769, 2119
AluI AGCT 6 cut(s) 287, 767, 1224, 1597, 1769, 2119
Alw21I GWGCWC 2 cut(s) 1226, 1858
Alw26I GTCTC 3 cut(s) 400, 632, 1804
Alw44I GTGCAC 1 cut(s) 1854
AlwI GGATC 7 cut(s) 25, 38, 414, 1148, 1161, 1295, 1459
AlwNI CAGNNNCTG 1 cut(s) 2206
Aor51HI AGCGCT 2 cut(s) 90, 1878
AoxI GGCC 2 cut(s) 706, 1098
ApaLI GTGCAC 1 cut(s) 1854
ApeKI GCWGC 4 cut(s) 5, 160, 614, 1361
ApoI RAATTY 4 cut(s) 122, 209, 568, 1499
AseI ATTAAT 1 cut(s) 267
Asp700I GAANNNNTTC 2 cut(s) 462, 1113
AspLEI GCGC 7 cut(s) 22, 91, 160, 362, 543, 619, 1879
AsuC2I CCSGG 2 cut(s) 35, 509
AsuHPI GGTGA 5 cut(s) 746, 791, 1262, 1907, 1960
AsuII TTCGAA 1 cut(s) 227
BaeGI GKGCMC 2 cut(s) 598, 1858
BaeI ACNNNNGTAYC 2 cut(s) 1023, 1056
BalI TGGCCA 1 cut(s) 708
BamHI GGATCC 2 cut(s) 30, 1153
BanI GGYRCC 3 cut(s) 19, 359, 1078
BanII GRGCYC 1 cut(s) 1226
BbrPI CACGTG 1 cut(s) 1343
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 2 cut(s) 1226, 1858
BbvCI CCTCAGC 2 cut(s) 525, 1642
BbvI GCAGC 4 cut(s) 17, 147, 626, 1348
BceAI ACGGC 4 cut(s) 10, 27, 223, 1085
BcgI CGANNNNNNTGC 4 cut(s) 56, 90, 217, 251
BciT130I CCWGG 2 cut(s) 299, 1560
BclI TGATCA 2 cut(s) 1267, 1912
BcnI CCSGG 2 cut(s) 35, 509
BcoDI GTCTC 3 cut(s) 400, 632, 1804
BcuI ACTAGT 1 cut(s) 1607
BfaI CTAG 5 cut(s) 992, 1598, 1608, 1649, 2013
BfmI CTRYAG 1 cut(s) 1590
BfoI RGCGCY 5 cut(s) 23, 92, 363, 620, 1880
BfuAI ACCTGC 1 cut(s) 62
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 9 cut(s) 6, 23, 161, 477, 522, 615, 1362, 1365, 2018
BlsI GCNGC 9 cut(s) 7, 24, 162, 478, 523, 616, 1363, 1366, 2019
Bme1390I CCNGG 4 cut(s) 35, 299, 509, 1560
BmeRI GACNNNNNGTC 1 cut(s) 181
BmgBI CACGTC 1 cut(s) 178
BmiI GGNNCC 7 cut(s) 21, 32, 296, 345, 361, 1080, 1155
BmrFI CCNGG 4 cut(s) 35, 299, 509, 1560
BmsI GCATC 7 cut(s) 568, 1378, 1589, 1810, 1892, 2029, 2247
BpiI GAAGAC 1 cut(s) 45
BplI GAGNNNNNCTC 2 cut(s) 380, 412
Bpu10I CCTNAGC 2 cut(s) 525, 1642
Bpu14I TTCGAA 1 cut(s) 227
BpuEI CTTGAG 3 cut(s) 38, 422, 1769
BpuMI CCSGG 2 cut(s) 35, 509
BsaAI YACGTR 1 cut(s) 1343
BsaHI GRCGYC 5 cut(s) 20, 171, 191, 360, 1011
BsaJI CCNNGG 5 cut(s) 26, 243, 298, 1074, 1284
BsaWI WCCGGW 1 cut(s) 2111
Bsc4I CCNNNNNNNGG 1 cut(s) 715
Bse118I RCCGGY 1 cut(s) 346
Bse1I ACTGG 1 cut(s) 979
Bse3DI GCAATG 1 cut(s) 726
BseBI CCWGG 2 cut(s) 299, 1560
BseDI CCNNGG 5 cut(s) 26, 243, 298, 1074, 1284
BseGI GGATG 9 cut(s) 207, 316, 583, 670, 1028, 1161, 1825, 2044, 2238
BseLI CCNNNNNNNGG 1 cut(s) 715
BseMI GCAATG 1 cut(s) 726
BseNI ACTGG 1 cut(s) 979
BseRI GAGGAG 5 cut(s) 38, 41, 114, 1790, 2248
BseSI GKGCMC 2 cut(s) 598, 1858
BseXI GCAGC 4 cut(s) 17, 147, 626, 1348
BsgI GTGCAG 2 cut(s) 1290, 1323
Bsh1236I CGCG 3 cut(s) 158, 541, 543
BshFI GGCC 2 cut(s) 708, 1100
BshNI GGYRCC 3 cut(s) 19, 359, 1078
BsiHKAI GWGCWC 2 cut(s) 1226, 1858
BsiSI CCGG 5 cut(s) 35, 347, 363, 508, 2112
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 1 cut(s) 715
BsmAI GTCTC 3 cut(s) 400, 632, 1804
BsmFI GGGAC 1 cut(s) 620
BsmI GAATGC 1 cut(s) 502
BsnI GGCC 2 cut(s) 708, 1100
Bsp119I TTCGAA 1 cut(s) 227
Bsp1286I GDGCHC 3 cut(s) 598, 1226, 1858
Bsp1407I TGTACA 1 cut(s) 1136
Bsp143I GATC 8 cut(s) 30, 406, 985, 1153, 1267, 1300, 1451, 1912
Bsp19I CCATGG 1 cut(s) 1074
BspANI GGCC 2 cut(s) 708, 1100
BspFNI CGCG 3 cut(s) 158, 541, 543
BspHI TCATGA 2 cut(s) 856, 979
BspLI GGNNCC 7 cut(s) 21, 32, 296, 345, 361, 1080, 1155
BspMAI CTGCAG 1 cut(s) 1594
BspMI ACCTGC 1 cut(s) 62
BspPI GGATC 7 cut(s) 25, 38, 414, 1148, 1161, 1295, 1459
BspT104I TTCGAA 1 cut(s) 227
BspT107I GGYRCC 3 cut(s) 19, 359, 1078
BsrBI CCGCTC 2 cut(s) 104, 148
BsrDI GCAATG 1 cut(s) 726
BsrFI RCCGGY 1 cut(s) 346
BsrGI TGTACA 1 cut(s) 1136
BsrI ACTGG 1 cut(s) 979
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 5 cut(s) 26, 243, 298, 1074, 1284
BssMI GATC 8 cut(s) 30, 406, 985, 1153, 1267, 1300, 1451, 1912
BssNI GRCGYC 5 cut(s) 20, 171, 191, 360, 1011
BssT1I CCWWGG 3 cut(s) 243, 1074, 1284
Bst2UI CCWGG 2 cut(s) 299, 1560
Bst4CI ACNGT 6 cut(s) 130, 894, 1066, 1403, 1465, 1851
Bst6I CTCTTC 4 cut(s) 630, 1104, 1119, 1422
BstACI GRCGYC 5 cut(s) 20, 171, 191, 360, 1011
BstAPI GCANNNNNTGC 3 cut(s) 160, 1577, 2216
BstAUI TGTACA 1 cut(s) 1136
BstBAI YACGTR 1 cut(s) 1343
BstBI TTCGAA 1 cut(s) 227
BstC8I GCNNGC 7 cut(s) 93, 156, 285, 630, 1369, 1881, 2022
BstDSI CCRYGG 2 cut(s) 26, 1074
BstF5I GGATG 9 cut(s) 207, 316, 583, 670, 1028, 1161, 1825, 2044, 2238
BstFNI CGCG 3 cut(s) 158, 541, 543
BstH2I RGCGCY 5 cut(s) 23, 92, 363, 620, 1880
BstHHI GCGC 7 cut(s) 22, 91, 160, 362, 543, 619, 1879
BstKTI GATC 8 cut(s) 33, 409, 988, 1156, 1270, 1303, 1454, 1915
BstMAI GTCTC 3 cut(s) 400, 632, 1804
BstMBI GATC 8 cut(s) 30, 406, 985, 1153, 1267, 1300, 1451, 1912
BstMWI GCNNNNNNNGC 8 cut(s) 11, 68, 101, 160, 625, 1577, 1876, 2216
BstNI CCWGG 2 cut(s) 299, 1560
BstSCI CCNGG 4 cut(s) 33, 297, 507, 1558
BstSFI CTRYAG 1 cut(s) 1590
BstSLI GKGCMC 2 cut(s) 598, 1858
BstUI CGCG 3 cut(s) 158, 541, 543
BstV1I GCAGC 4 cut(s) 17, 147, 626, 1348
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 3 cut(s) 30, 1153, 1300
BstYI RGATCY 3 cut(s) 30, 1153, 1300
BsuRI GGCC 2 cut(s) 708, 1100
BtgI CCRYGG 2 cut(s) 26, 1074
BtrI CACGTC 1 cut(s) 178
BtsCI GGATG 9 cut(s) 207, 316, 583, 670, 1028, 1161, 1825, 2044, 2238
BtsIMutI CAGTG 1 cut(s) 1856
BveI ACCTGC 1 cut(s) 62
Cac8I GCNNGC 7 cut(s) 93, 156, 285, 630, 1369, 1881, 2022
CaiI CAGNNNCTG 1 cut(s) 2206
CciI TCATGA 2 cut(s) 856, 979
CfoI GCGC 7 cut(s) 22, 91, 160, 362, 543, 619, 1879
Cfr10I RCCGGY 1 cut(s) 346
CseI GACGC 4 cut(s) 179, 180, 1019, 1715
Csp6I GTAC 2 cut(s) 1040, 1137
CviQI GTAC 2 cut(s) 1040, 1137
DinI GGCGCC 2 cut(s) 21, 361
DpnI GATC 8 cut(s) 32, 408, 987, 1155, 1269, 1302, 1453, 1914
DpnII GATC 8 cut(s) 30, 406, 985, 1153, 1267, 1300, 1451, 1912
DriI GACNNNNNGTC 1 cut(s) 181
EaeI YGGCCR 2 cut(s) 706, 1098
Eam1104I CTCTTC 4 cut(s) 630, 1104, 1119, 1422
Eam1105I GACNNNNNGTC 1 cut(s) 181
EarI CTCTTC 4 cut(s) 630, 1104, 1119, 1422
EciI GGCGGA 2 cut(s) 399, 435
Ecl136II GAGCTC 1 cut(s) 1224
Eco130I CCWWGG 3 cut(s) 243, 1074, 1284
Eco24I GRGCYC 1 cut(s) 1226
Eco32I GATATC 3 cut(s) 1180, 1732, 1786
Eco47III AGCGCT 2 cut(s) 90, 1878
Eco53kI GAGCTC 1 cut(s) 1224
Eco72I CACGTG 1 cut(s) 1343
EcoICRI GAGCTC 1 cut(s) 1224
EcoRI GAATTC 2 cut(s) 209, 568
EcoRII CCWGG 2 cut(s) 297, 1558
EcoRV GATATC 3 cut(s) 1180, 1732, 1786
EcoT14I CCWWGG 3 cut(s) 243, 1074, 1284
EcoT38I GRGCYC 1 cut(s) 1226
EgeI GGCGCC 2 cut(s) 21, 361
EheI GGCGCC 2 cut(s) 21, 361
ErhI CCWWGG 3 cut(s) 243, 1074, 1284
FalI AAGNNNNNCTT 4 cut(s) 1881, 1913, 2116, 2148
FaqI GGGAC 1 cut(s) 620
FauI CCCGC 3 cut(s) 125, 135, 536
FbaI TGATCA 2 cut(s) 1267, 1912
FblI GTMKAC 1 cut(s) 1792
Fnu4HI GCNGC 9 cut(s) 6, 23, 161, 477, 522, 615, 1362, 1365, 2018
FokI GGATG 9 cut(s) 214, 323, 590, 677, 1015, 1148, 1832, 2051, 2225
FriOI GRGCYC 1 cut(s) 1226
Fsp4HI GCNGC 9 cut(s) 6, 23, 161, 477, 522, 615, 1362, 1365, 2018
FspBI CTAG 5 cut(s) 992, 1598, 1608, 1649, 2013
GlaI GCGC 7 cut(s) 21, 90, 159, 361, 542, 618, 1878
GluI GCNGC 9 cut(s) 6, 23, 161, 477, 522, 615, 1362, 1365, 2018
HaeII RGCGCY 5 cut(s) 23, 92, 363, 620, 1880
HaeIII GGCC 2 cut(s) 708, 1100
HapII CCGG 5 cut(s) 35, 347, 363, 508, 2112
HgaI GACGC 4 cut(s) 179, 180, 1019, 1715
HhaI GCGC 7 cut(s) 22, 91, 160, 362, 543, 619, 1879
Hin1I GRCGYC 5 cut(s) 20, 171, 191, 360, 1011
Hin6I GCGC 7 cut(s) 20, 89, 158, 360, 541, 617, 1877
HinP1I GCGC 7 cut(s) 20, 89, 158, 360, 541, 617, 1877
HincII GTYRAC 1 cut(s) 884
HindII GTYRAC 1 cut(s) 884
HpaII CCGG 5 cut(s) 35, 347, 363, 508, 2112
HphI GGTGA 5 cut(s) 746, 791, 1262, 1907, 1960
Hpy166II GTNNAC 3 cut(s) 884, 1793, 1856
Hpy8I GTNNAC 3 cut(s) 884, 1793, 1856
Hpy99I CGWCG 5 cut(s) 13, 173, 179, 187, 236
HpyAV CCTTC 3 cut(s) 428, 731, 1176
HpyCH4III ACNGT 6 cut(s) 130, 894, 1066, 1403, 1465, 1851
HpyCH4IV ACGT 5 cut(s) 177, 231, 609, 1342, 1980
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 68, 101, 160, 625, 1577, 1876, 2216
HpySE526I ACGT 5 cut(s) 177, 231, 609, 1342, 1980
Hsp92I GRCGYC 5 cut(s) 20, 171, 191, 360, 1011
HspAI GCGC 7 cut(s) 20, 89, 158, 360, 541, 617, 1877
KasI GGCGCC 2 cut(s) 19, 359
Ksp22I TGATCA 2 cut(s) 1267, 1912
Kzo9I GATC 8 cut(s) 30, 406, 985, 1153, 1267, 1300, 1451, 1912
LmnI GCTCC 6 cut(s) 101, 145, 343, 1075, 1093, 2235
Lsp1109I GCAGC 4 cut(s) 17, 147, 626, 1348
LweI GCATC 7 cut(s) 568, 1378, 1589, 1810, 1892, 2029, 2247
MaeI CTAG 5 cut(s) 992, 1598, 1608, 1649, 2013
MaeII ACGT 5 cut(s) 177, 231, 609, 1342, 1980
MalI GATC 8 cut(s) 32, 408, 987, 1155, 1269, 1302, 1453, 1914
MbiI CCGCTC 2 cut(s) 104, 148
MboI GATC 8 cut(s) 30, 406, 985, 1153, 1267, 1300, 1451, 1912
MflI RGATCY 3 cut(s) 30, 1153, 1300
MhlI GDGCHC 3 cut(s) 598, 1226, 1858
MlsI TGGCCA 1 cut(s) 708
MluNI TGGCCA 1 cut(s) 708
Mly113I GGCGCC 2 cut(s) 20, 360
MlyI GAGTC 2 cut(s) 1787, 1806
MmeI TCCRAC 5 cut(s) 165, 186, 307, 555, 1824
Mox20I TGGCCA 1 cut(s) 708
MroXI GAANNNNTTC 2 cut(s) 462, 1113
MscI TGGCCA 1 cut(s) 708
MseI TTAA 6 cut(s) 267, 549, 654, 1586, 2123, 2181
MslI CAYNNNNRTG 3 cut(s) 601, 861, 1818
Msp20I TGGCCA 1 cut(s) 708
MspA1I CMGCKG 3 cut(s) 524, 632, 1769
MspI CCGG 5 cut(s) 35, 347, 363, 508, 2112
MspR9I CCNGG 4 cut(s) 35, 299, 509, 1560
Mva1269I GAATGC 1 cut(s) 502
MvaI CCWGG 2 cut(s) 299, 1560
MvnI CGCG 3 cut(s) 158, 541, 543
MwoI GCNNNNNNNGC 8 cut(s) 11, 68, 101, 160, 625, 1577, 1876, 2216
NarI GGCGCC 2 cut(s) 20, 360
NciI CCSGG 2 cut(s) 35, 509
NcoI CCATGG 1 cut(s) 1074
NdeII GATC 8 cut(s) 30, 406, 985, 1153, 1267, 1300, 1451, 1912
NlaIV GGNNCC 7 cut(s) 21, 32, 296, 345, 361, 1080, 1155
NspV TTCGAA 1 cut(s) 227
PagI TCATGA 2 cut(s) 856, 979
PaqCI CACCTGC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 3 cut(s) 23, 174, 188
PctI GAATGC 1 cut(s) 502
PdmI GAANNNNTTC 2 cut(s) 462, 1113
PfeI GAWTC 8 cut(s) 111, 686, 800, 829, 1048, 1661, 1673, 2065
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 9 cut(s) 7, 24, 162, 478, 523, 616, 1363, 1366, 2019
PleI GAGTC 2 cut(s) 1786, 1805
PluTI GGCGCC 2 cut(s) 23, 363
PmaCI CACGTG 1 cut(s) 1343
PmlI CACGTG 1 cut(s) 1343
PpsI GAGTC 2 cut(s) 1786, 1805
Ppu21I YACGTR 1 cut(s) 1343
PshBI ATTAAT 1 cut(s) 267
PsiI TTATAA 1 cut(s) 2030
Psp124BI GAGCTC 1 cut(s) 1226
Psp6I CCWGG 2 cut(s) 297, 1558
PspCI CACGTG 1 cut(s) 1343
PspGI CCWGG 2 cut(s) 297, 1558
PspN4I GGNNCC 7 cut(s) 21, 32, 296, 345, 361, 1080, 1155
PstI CTGCAG 1 cut(s) 1594
PstNI CAGNNNCTG 1 cut(s) 2206
PsuI RGATCY 3 cut(s) 30, 1153, 1300
PvuII CAGCTG 1 cut(s) 1769
RsaI GTAC 2 cut(s) 1041, 1138
RsaNI GTAC 2 cut(s) 1040, 1137
RseI CAYNNNNRTG 3 cut(s) 601, 861, 1818
SacI GAGCTC 1 cut(s) 1226
SaqAI TTAA 6 cut(s) 267, 549, 654, 1586, 2123, 2181
SatI GCNGC 9 cut(s) 6, 23, 161, 477, 522, 615, 1362, 1365, 2018
Sau3AI GATC 8 cut(s) 30, 406, 985, 1153, 1267, 1300, 1451, 1912
SchI GAGTC 2 cut(s) 1787, 1806
ScrFI CCNGG 4 cut(s) 35, 299, 509, 1560
SduI GDGCHC 3 cut(s) 598, 1226, 1858
SfaNI GCATC 7 cut(s) 568, 1378, 1589, 1810, 1892, 2029, 2247
SfcI CTRYAG 1 cut(s) 1590
SfoI GGCGCC 2 cut(s) 21, 361
SfuI TTCGAA 1 cut(s) 227
SmiMI CAYNNNNRTG 3 cut(s) 601, 861, 1818
SmlI CTYRAG 3 cut(s) 53, 401, 1748
SmoI CTYRAG 3 cut(s) 53, 401, 1748
SpeI ACTAGT 1 cut(s) 1607
SspDI GGCGCC 2 cut(s) 19, 359
SspMI CTAG 5 cut(s) 992, 1598, 1608, 1649, 2013
SstI GAGCTC 1 cut(s) 1226
StyD4I CCNGG 4 cut(s) 33, 297, 507, 1558
StyI CCWWGG 3 cut(s) 243, 1074, 1284
TaaI ACNGT 6 cut(s) 130, 894, 1066, 1403, 1465, 1851
TaiI ACGT 5 cut(s) 180, 234, 612, 1345, 1983
TaqI TCGA 9 cut(s) 66, 213, 227, 261, 669, 827, 960, 1734, 2135
TatI WGTACW 1 cut(s) 1136
TauI GCSGC 5 cut(s) 25, 479, 524, 1367, 2020
TfiI GAWTC 8 cut(s) 111, 686, 800, 829, 1048, 1661, 1673, 2065
Tru1I TTAA 6 cut(s) 267, 549, 654, 1586, 2123, 2181
Tru9I TTAA 6 cut(s) 267, 549, 654, 1586, 2123, 2181
TscAI CASTG 1 cut(s) 1856
TseI GCWGC 4 cut(s) 5, 160, 614, 1361
TspRI CASTG 1 cut(s) 1856
VneI GTGCAC 1 cut(s) 1854
VspI ATTAAT 1 cut(s) 267
XapI RAATTY 4 cut(s) 122, 209, 568, 1499
XmiI GTMKAC 1 cut(s) 1792
XmnI GAANNNNTTC 2 cut(s) 462, 1113
XspI CTAG 5 cut(s) 992, 1598, 1608, 1649, 2013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.