FvH4_1g12730

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0017219
Physical Location & Seq
Forward (+)
7010041 .. 7011949
1909 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g12730.t1

Sequence Viewer

Length: 1152 bp
ATGAATGCAAGAGAAATGTTTGTCAAAATAGCAGAAGCATTTGTGGAGTTGTACAATTATGTTAGAGGGCCTATGGTGCCGCAAGCAATTCAGTTAAGGGATGCAGTAGTGAAGATTCATGAAAGCTTGGCTGGTCAAATTGCAGACTCAGAATTGATAAGTAGTGTTATCGAATGGTTTAATACGTTTCATAACATTTATGTCGCACTAGGATTGGTTGGTTTGGTGATCTCCTTTGTTCTAGGATTGCTTTGGAAAGTTTTAGCTGTGGTAGTTTCTGCTTTAGGTGTATGGATTTCCTATTTGATTTGGAGAGACCATAATTGGCACAAGCTGTTCCTGACAGACGAAAAAGGATTAGCATTTTTTGCAAGTACTTGGCTTGGGTTATTGCTACTCTACCGAGCACAAGGTCTGCTTGGCAAAGTTTGTCTTTTGGGAATAGAAACCCTAGTAATATGGGTGATGCAGTATTCCATGTCGACGACGACGAATATGGAAATCCCTTGGGATGATTCTAATATCCATGAAATTATCTCCCGAGCTGTTCCACCGGTGCCCCATTATTCTTCTCCGCATGACCTCTACCGCAGTCTCTGCGCTTCCCCCATCTTCCTTGACGATGGCTACTTGAGTTTGGCGTATGATCGGGCGACTGGTAAGAAATGCTGGATGATGGGAGCCAGGCTTTTACTTATTGATTTGGCAGAAATTCCAGACTACTGGAACTGGATATCTCTGCCTGAATCCAGGTTTTCCCAAGTGGCTGAGCTCAGATTTGTACGTCGTCTTAAGATCAAGGGATCCATTTCTACCTATATGTTGTCACCAAGAACAACCTATGTAGCTTACTTTGTATACAAGTTTTCAAATACATCTCGATCTGGTTTTCATGAAAGGCCTGTTAGCTTGCGTGTAGTTCGTGTTGTTAATACTCAACGCCAAGTTGTGGCGCATCATAATCGTGTGTTCCTGATAGGAGAGATGGCTTCACAAGCTCGAACGAGAAGTGATGGATGGATGGAGATTGAGTTCGGTGAAATCTCCTACATTGATGATAGTGCTACTATAGAGTGTATACTAGAGGGATCTGAAAGTTTTGAGGGAAAAAGAAGTCTCATTGTGGAAGGTATTGAGCTCAGGCCTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

384

Amino Acids

43.78

Weight (kDa)

6.56

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 221 - 382 2.6e-39 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017219)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12730
rosa_chinensis RchiOBHm_Chr2g0100791
rosa_laevigata RLG00000017010
rosa_multiflora Rmu_sc0001187.1_g000001 Rmu_sc0001659.1_g000017
rosa_roxburghii Rroxscaffold_2G00141860
rosa_rugosa Rorug02G0092700 Rorug02G0092700 Rorug02G0092800
rosa_samantha Rh2AG140500 Rh2BG145400 Rh2CG146300 Rh2DG145800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 76, 556
AccB7I CCANNNNNTGG 1 cut(s) 949
AccI GTMKAC 3 cut(s) 482, 858, 1078
AciI CCGC 3 cut(s) 80, 575, 589
AclWI GGATC 3 cut(s) 798, 811, 1096
AcsI RAATTY 1 cut(s) 711
AfaI GTAC 3 cut(s) 53, 376, 783
AfiI CCNNNNNNNGG 1 cut(s) 949
AflII CTTAAG 1 cut(s) 791
AgeI ACCGGT 1 cut(s) 553
AgsI TTSAA 1 cut(s) 870
AjnI CCWGG 2 cut(s) 683, 749
AloI GAACNNNNNNTCC 2 cut(s) 1016, 1048
AluBI AGCT 9 cut(s) 126, 266, 334, 545, 772, 848, 909, 998, 1138
AluI AGCT 9 cut(s) 126, 266, 334, 545, 772, 848, 909, 998, 1138
Alw21I GWGCWC 3 cut(s) 409, 774, 1140
Alw26I GTCTC 3 cut(s) 309, 599, 1121
AlwI GGATC 3 cut(s) 798, 811, 1096
AlwNI CAGNNNCTG 1 cut(s) 597
Ama87I CYCGRG 1 cut(s) 540
AoxI GGCC 3 cut(s) 68, 899, 1142
ApoI RAATTY 1 cut(s) 711
AsiGI ACCGGT 1 cut(s) 553
AspLEI GCGC 2 cut(s) 602, 955
AspS9I GGNCC 1 cut(s) 68
AsuHPI GGTGA 4 cut(s) 238, 475, 819, 1049
AvaI CYCGRG 1 cut(s) 540
BaeGI GKGCMC 1 cut(s) 561
BamHI GGATCC 1 cut(s) 803
BanI GGYRCC 2 cut(s) 76, 556
BanII GRGCYC 2 cut(s) 774, 1140
Bbv12I GWGCWC 3 cut(s) 409, 774, 1140
BccI CCATC 7 cut(s) 617, 617, 670, 979, 1007, 1011, 1015
BcgI CGANNNNNNTGC 2 cut(s) 944, 978
BciT130I CCWGG 2 cut(s) 685, 751
BcoDI GTCTC 3 cut(s) 309, 599, 1121
BfaI CTAG 5 cut(s) 209, 242, 452, 1082, 1146
BfmI CTRYAG 1 cut(s) 1068
BfrI CTTAAG 1 cut(s) 791
BisI GCNGC 1 cut(s) 80
BlpI GCTNAGC 1 cut(s) 768
BlsI GCNGC 1 cut(s) 81
BmcAI AGTACT 1 cut(s) 376
Bme1390I CCNGG 2 cut(s) 685, 751
BmeT110I CYCGRG 1 cut(s) 540
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 4 cut(s) 78, 558, 682, 805
BmrFI CCNGG 2 cut(s) 685, 751
BmsI GCATC 3 cut(s) 91, 456, 964
Bpu10I CCTNAGC 1 cut(s) 1139
Bpu1102I GCTNAGC 1 cut(s) 768
BpuEI CTTGAG 1 cut(s) 652
BsaI GGTCTC 1 cut(s) 309
BsaJI CCNNGG 1 cut(s) 506
BsaWI WCCGGW 1 cut(s) 553
BsaXI ACNNNNNCTCC 2 cut(s) 1016, 1046
Bsc4I CCNNNNNNNGG 1 cut(s) 949
Bse118I RCCGGY 1 cut(s) 553
Bse1I ACTGG 3 cut(s) 661, 728, 734
BseBI CCWGG 2 cut(s) 685, 751
BseDI CCNNGG 1 cut(s) 506
BseGI GGATG 5 cut(s) 106, 517, 678, 1022, 1026
BseLI CCNNNNNNNGG 1 cut(s) 949
BseMII CTCAG 3 cut(s) 162, 759, 787
BseNI ACTGG 3 cut(s) 661, 728, 734
BseSI GKGCMC 1 cut(s) 561
BshFI GGCC 3 cut(s) 70, 901, 1144
BshNI GGYRCC 2 cut(s) 76, 556
BshTI ACCGGT 1 cut(s) 553
BsiHKAI GWGCWC 3 cut(s) 409, 774, 1140
BsiHKCI CYCGRG 1 cut(s) 540
BsiSI CCGG 1 cut(s) 554
BslI CCNNNNNNNGG 1 cut(s) 949
BsmAI GTCTC 3 cut(s) 309, 599, 1121
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 3 cut(s) 70, 901, 1144
Bso31I GGTCTC 1 cut(s) 309
BsoBI CYCGRG 1 cut(s) 540
Bsp1286I GDGCHC 4 cut(s) 409, 561, 774, 1140
Bsp1407I TGTACA 1 cut(s) 51
Bsp143I GATC 6 cut(s) 228, 646, 795, 803, 881, 1088
Bsp1720I GCTNAGC 1 cut(s) 768
BspACI CCGC 3 cut(s) 80, 575, 589
BspANI GGCC 3 cut(s) 70, 901, 1144
BspCNI CTCAG 4 cut(s) 161, 760, 786, 1152
BspHI TCATGA 2 cut(s) 118, 892
BspLI GGNNCC 4 cut(s) 78, 558, 682, 805
BspPI GGATC 3 cut(s) 798, 811, 1096
BspT107I GGYRCC 2 cut(s) 76, 556
BspTI CTTAAG 1 cut(s) 791
BspTNI GGTCTC 1 cut(s) 309
BsrFI RCCGGY 1 cut(s) 553
BsrGI TGTACA 1 cut(s) 51
BsrI ACTGG 3 cut(s) 661, 728, 734
BssAI RCCGGY 1 cut(s) 553
BssECI CCNNGG 1 cut(s) 506
BssMI GATC 6 cut(s) 228, 646, 795, 803, 881, 1088
BssNAI GTATAC 2 cut(s) 859, 1079
BssT1I CCWWGG 1 cut(s) 506
Bst1107I GTATAC 2 cut(s) 859, 1079
Bst2UI CCWGG 2 cut(s) 685, 751
BstAFI CTTAAG 1 cut(s) 791
BstAPI GCANNNNNTGC 2 cut(s) 368, 597
BstAUI TGTACA 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 84, 911
BstDEI CTNAG 4 cut(s) 148, 768, 773, 1139
BstF5I GGATG 5 cut(s) 106, 517, 678, 1022, 1026
BstHHI GCGC 2 cut(s) 602, 955
BstKTI GATC 6 cut(s) 231, 649, 798, 806, 884, 1091
BstMAI GTCTC 3 cut(s) 309, 599, 1121
BstMBI GATC 6 cut(s) 228, 646, 795, 803, 881, 1088
BstMWI GCNNNNNNNGC 4 cut(s) 76, 368, 597, 995
BstNI CCWGG 2 cut(s) 685, 751
BstSCI CCNGG 2 cut(s) 683, 749
BstSFI CTRYAG 1 cut(s) 1068
BstSLI GKGCMC 1 cut(s) 561
BstX2I RGATCY 2 cut(s) 803, 1088
BstXI CCANNNNNNTGG 1 cut(s) 723
BstYI RGATCY 2 cut(s) 803, 1088
BstZ17I GTATAC 2 cut(s) 859, 1079
BsuRI GGCC 3 cut(s) 70, 901, 1144
BtsCI GGATG 5 cut(s) 106, 517, 678, 1022, 1026
Cac8I GCNNGC 2 cut(s) 84, 911
CaiI CAGNNNCTG 1 cut(s) 597
CciI TCATGA 2 cut(s) 118, 892
CfoI GCGC 2 cut(s) 602, 955
Cfr10I RCCGGY 1 cut(s) 553
Cfr13I GGNCC 1 cut(s) 68
Csp6I GTAC 3 cut(s) 52, 375, 782
CspAI ACCGGT 1 cut(s) 553
CviAII CATG 5 cut(s) 119, 478, 527, 578, 893
CviQI GTAC 3 cut(s) 52, 375, 782
DdeI CTNAG 4 cut(s) 148, 768, 773, 1139
DpnI GATC 6 cut(s) 230, 648, 797, 805, 883, 1090
DpnII GATC 6 cut(s) 228, 646, 795, 803, 881, 1088
Ecl136II GAGCTC 2 cut(s) 772, 1138
Eco130I CCWWGG 1 cut(s) 506
Eco147I AGGCCT 2 cut(s) 901, 1144
Eco24I GRGCYC 2 cut(s) 774, 1140
Eco31I GGTCTC 1 cut(s) 309
Eco32I GATATC 1 cut(s) 735
Eco53kI GAGCTC 2 cut(s) 772, 1138
Eco88I CYCGRG 1 cut(s) 540
EcoICRI GAGCTC 2 cut(s) 772, 1138
EcoO109I RGGNCCY 1 cut(s) 68
EcoRII CCWGG 2 cut(s) 683, 749
EcoRV GATATC 1 cut(s) 735
EcoT14I CCWWGG 1 cut(s) 506
EcoT38I GRGCYC 2 cut(s) 774, 1140
ErhI CCWWGG 1 cut(s) 506
FaeI CATG 5 cut(s) 122, 481, 530, 581, 896
FalI AAGNNNNNCTT 4 cut(s) 402, 434, 417, 449
FatI CATG 5 cut(s) 118, 477, 526, 577, 892
FblI GTMKAC 3 cut(s) 482, 858, 1078
Fnu4HI GCNGC 1 cut(s) 80
FokI GGATG 5 cut(s) 113, 524, 685, 1029, 1033
FriOI GRGCYC 2 cut(s) 774, 1140
Fsp4HI GCNGC 1 cut(s) 80
FspBI CTAG 5 cut(s) 209, 242, 452, 1082, 1146
GlaI GCGC 2 cut(s) 601, 954
GluI GCNGC 1 cut(s) 80
HaeIII GGCC 3 cut(s) 70, 901, 1144
HapII CCGG 1 cut(s) 554
HhaI GCGC 2 cut(s) 602, 955
Hin1II CATG 5 cut(s) 122, 481, 530, 581, 896
Hin6I GCGC 2 cut(s) 600, 953
HinP1I GCGC 2 cut(s) 600, 953
HincII GTYRAC 1 cut(s) 483
HindII GTYRAC 1 cut(s) 483
HindIII AAGCTT 1 cut(s) 124
HinfI GANTC 4 cut(s) 115, 146, 515, 746
HpaII CCGG 1 cut(s) 554
HphI GGTGA 4 cut(s) 238, 475, 819, 1049
Hpy166II GTNNAC 3 cut(s) 483, 859, 1079
Hpy188I TCNGA 3 cut(s) 151, 776, 1093
Hpy188III TCNNGA 7 cut(s) 119, 340, 540, 716, 879, 893, 973
Hpy8I GTNNAC 3 cut(s) 483, 859, 1079
Hpy99I CGWCG 4 cut(s) 487, 490, 493, 789
HpyAV CCTTC 1 cut(s) 1121
HpyCH4IV ACGT 2 cut(s) 185, 784
HpyCH4V TGCA 5 cut(s) 8, 104, 143, 371, 469
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 368, 597, 995
HpyF3I CTNAG 4 cut(s) 148, 768, 773, 1139
HpySE526I ACGT 2 cut(s) 185, 784
Hsp92II CATG 5 cut(s) 122, 481, 530, 581, 896
HspAI GCGC 2 cut(s) 600, 953
Kzo9I GATC 6 cut(s) 228, 646, 795, 803, 881, 1088
LmnI GCTCC 1 cut(s) 680
LweI GCATC 3 cut(s) 91, 456, 964
MaeI CTAG 5 cut(s) 209, 242, 452, 1082, 1146
MaeII ACGT 2 cut(s) 185, 784
MaeIII GTNAC 1 cut(s) 825
MalI GATC 6 cut(s) 230, 648, 797, 805, 883, 1090
MboI GATC 6 cut(s) 228, 646, 795, 803, 881, 1088
MboII GAAGA 3 cut(s) 124, 561, 604
MflI RGATCY 2 cut(s) 803, 1088
MhlI GDGCHC 4 cut(s) 409, 561, 774, 1140
MluCI AATT 7 cut(s) 55, 87, 138, 152, 322, 531, 711
MlyI GAGTC 1 cut(s) 140
MnlI CCTC 4 cut(s) 59, 593, 1078, 1096
MseI TTAA 4 cut(s) 95, 180, 792, 930
MslI CAYNNNNRTG 1 cut(s) 963
MspCI CTTAAG 1 cut(s) 791
MspI CCGG 1 cut(s) 554
MspR9I CCNGG 2 cut(s) 685, 751
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 2 cut(s) 685, 751
MwoI GCNNNNNNNGC 4 cut(s) 76, 368, 597, 995
NdeII GATC 6 cut(s) 228, 646, 795, 803, 881, 1088
NlaIII CATG 5 cut(s) 122, 481, 530, 581, 896
NlaIV GGNNCC 4 cut(s) 78, 558, 682, 805
NmuCI GTSAC 1 cut(s) 825
PagI TCATGA 2 cut(s) 118, 892
PceI AGGCCT 2 cut(s) 901, 1144
PcsI WCGNNNNNNNCGW 1 cut(s) 488
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 3 cut(s) 115, 515, 746
PflMI CCANNNNNTGG 1 cut(s) 949
PinAI ACCGGT 1 cut(s) 553
PkrI GCNGC 1 cut(s) 81
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
Psp124BI GAGCTC 2 cut(s) 774, 1140
Psp6I CCWGG 2 cut(s) 683, 749
PspGI CCWGG 2 cut(s) 683, 749
PspN4I GGNNCC 4 cut(s) 78, 558, 682, 805
PspPI GGNCC 1 cut(s) 68
PstNI CAGNNNCTG 1 cut(s) 597
PsuI RGATCY 2 cut(s) 803, 1088
RsaI GTAC 3 cut(s) 53, 376, 783
RsaNI GTAC 3 cut(s) 52, 375, 782
RseI CAYNNNNRTG 1 cut(s) 963
SacI GAGCTC 2 cut(s) 774, 1140
SalI GTCGAC 1 cut(s) 481
SaqAI TTAA 4 cut(s) 95, 180, 792, 930
SatI GCNGC 1 cut(s) 80
Sau3AI GATC 6 cut(s) 228, 646, 795, 803, 881, 1088
Sau96I GGNCC 1 cut(s) 68
ScaI AGTACT 1 cut(s) 376
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 685, 751
SduI GDGCHC 4 cut(s) 409, 561, 774, 1140
SfaNI GCATC 3 cut(s) 91, 456, 964
SfcI CTRYAG 1 cut(s) 1068
SgrAI CRCCGGYG 1 cut(s) 553
SmiMI CAYNNNNRTG 1 cut(s) 963
SmlI CTYRAG 2 cut(s) 631, 791
SmoI CTYRAG 2 cut(s) 631, 791
Sse9I AATT 7 cut(s) 55, 87, 138, 152, 322, 531, 711
SseBI AGGCCT 2 cut(s) 901, 1144
SsiI CCGC 3 cut(s) 80, 575, 589
SspMI CTAG 5 cut(s) 209, 242, 452, 1082, 1146
SstI GAGCTC 2 cut(s) 774, 1140
StuI AGGCCT 2 cut(s) 901, 1144
StyD4I CCNGG 2 cut(s) 683, 749
StyI CCWWGG 1 cut(s) 506
TaiI ACGT 2 cut(s) 188, 787
TaqI TCGA 4 cut(s) 171, 482, 880, 1000
TasI AATT 7 cut(s) 55, 87, 138, 152, 322, 531, 711
TatI WGTACW 2 cut(s) 51, 374
TauI GCSGC 1 cut(s) 82
TfiI GAWTC 3 cut(s) 115, 515, 746
Tru1I TTAA 4 cut(s) 95, 180, 792, 930
Tru9I TTAA 4 cut(s) 95, 180, 792, 930
TseFI GTSAC 1 cut(s) 825
Tsp45I GTSAC 1 cut(s) 825
TspDTI ATGAA 7 cut(s) 17, 107, 135, 179, 543, 881, 909
Van91I CCANNNNNTGG 1 cut(s) 949
Vha464I CTTAAG 1 cut(s) 791
XapI RAATTY 1 cut(s) 711
XmiI GTMKAC 3 cut(s) 482, 858, 1078
XspI CTAG 5 cut(s) 209, 242, 452, 1082, 1146
ZrmI AGTACT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.