Rh2CG146300

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0017219
Physical Location & Seq
Forward (+)
12830512 .. 12832616
2105 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG146300.1

Sequence Viewer

Length: 1146 bp
ATGAATGCAAGAGAAATGTTTCTCGCAATAGCAGGAGCATTTGTGGAGTTGTACAATTATGTTAGAGGGCCTATGGTGCCGCAAGCAACTCAATTAAGGGATGCAGTAGTGAGGATTCATGAACGCTGGGCAGGTCAAATTGCAGAGCCAGAATTGATGATCAGTAGTATTCTCGAATTGCTTAATACGTTCCAAAACATTTATACCGGACTAGGATTGGTTTGTTTGGTGATCTCCTTTGTTCTAGGATTGCTTTGGAGAGCGTTTGTTCTGGTGATTTCTGCTTTGGGTGTATGGATTTCCTATTTGATTTTGACAGACCATGATTGGCACGGGCTGTTTCTGACAGACGAAAAAGGCTTTGCACTTTTTGCAAGTGCTTGGCTTGGTTTACTGCTACTCTACCAAGCACAAAGTCTGCTTGCCAAAGTTTGCCTTTGCGGTATAGAAACCCTAGTAATATGGGTAATGTATTCGATGACGACTAATAAGGAAATCCCTTGGGATCATTCTAGTTTCCCTGAAATCATCTCCCGATCTATTGATCCGGTGCCCCATTATTCCTCTCCGTCAGACCTCTACCGCAGTCTCTGTGTTTCCCCCATCTTCCTTGACGAAGGCCATTTGAGCTTTGCGTATGATCGGGCGACTGGTAAAAAATGCTGGATGCTGGGAGCCAGGCTTTTACTTATTGATTTGGCAGAAATTCCAGAATACTGGAACTGGAGTTCTGTACCCGAATCCAGGTTCTCCCAAGTGGCTGAGCTCAGATTTGTATATAGTCTTAAGATCAAGGGATCTATAGCGACATATATGTTGTCCCCGAGAACAACTTATGTAGCTTACCTTGTATATAAGTTTCCAAATATATCTCGATCTGGTTTTCATGAAAGCCCTGTTAGCTTGCGTGTAGTGCGTGTAGTTAATATTCAACGCAGAGTTGTAGCTCAACATAATCGTGTCTTCCTGATAGGAGATATGGCTTCACAAGCTCGAAGGAGAAATGACGGATGGATGGAGATTGAGTTCGGTGAAATCTCCTATATTGAAGATAATGCTACTATAGAGTGTATTCTAGAGGGATCTGAAAGTTTTGTGAGAAAAAGAGGTCTCATTGTGGAAGGTATTGAGTTTAGGCCTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

381

Amino Acids

43.51

Weight (kDa)

6.27

Isoelectric Point (pI)

59.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 219 - 380 1.1e-39 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017219)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12730
rosa_chinensis RchiOBHm_Chr2g0100791
rosa_laevigata RLG00000017010
rosa_multiflora Rmu_sc0001187.1_g000001 Rmu_sc0001659.1_g000017
rosa_roxburghii Rroxscaffold_2G00141860
rosa_rugosa Rorug02G0092700 Rorug02G0092700 Rorug02G0092800
rosa_samantha Rh2AG140500 Rh2BG145400 Rh2CG146300 Rh2DG145800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 122
AccB1I GGYRCC 2 cut(s) 76, 550
AciI CCGC 3 cut(s) 80, 441, 583
AclWI GGATC 4 cut(s) 513, 539, 805, 1090
AcsI RAATTY 1 cut(s) 705
AfaI GTAC 2 cut(s) 53, 735
AfiI CCNNNNNNNGG 1 cut(s) 744
AflII CTTAAG 1 cut(s) 785
AgsI TTSAA 2 cut(s) 932, 1049
AjnI CCWGG 2 cut(s) 677, 743
AloI GAACNNNNNNTCC 4 cut(s) 712, 744, 1010, 1042
AluBI AGCT 6 cut(s) 630, 766, 842, 903, 947, 992
AluI AGCT 6 cut(s) 630, 766, 842, 903, 947, 992
Alw21I GWGCWC 1 cut(s) 768
Alw26I GTCTC 2 cut(s) 593, 1115
AlwI GGATC 4 cut(s) 513, 539, 805, 1090
AlwNI CAGNNNCTG 1 cut(s) 591
Ama87I CYCGRG 1 cut(s) 823
AoxI GGCC 3 cut(s) 68, 619, 1136
ApoI RAATTY 1 cut(s) 705
Asp700I GAANNNNTTC 1 cut(s) 18
AspS9I GGNCC 1 cut(s) 68
AsuHPI GGTGA 3 cut(s) 241, 286, 1043
AvaI CYCGRG 1 cut(s) 823
BaeGI GKGCMC 1 cut(s) 555
BanI GGYRCC 2 cut(s) 76, 550
BanII GRGCYC 1 cut(s) 768
BbsI GAAGAC 1 cut(s) 955
Bbv12I GWGCWC 1 cut(s) 768
BccI CCATC 3 cut(s) 611, 1005, 1009
BciT130I CCWGG 2 cut(s) 679, 745
BclI TGATCA 1 cut(s) 159
BcoDI GTCTC 2 cut(s) 593, 1115
BfaI CTAG 6 cut(s) 212, 245, 455, 513, 1076, 1140
BfmI CTRYAG 2 cut(s) 801, 1062
BfrI CTTAAG 1 cut(s) 785
BfuAI ACCTGC 1 cut(s) 122
BisI GCNGC 1 cut(s) 80
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 1 cut(s) 81
Bme1390I CCNGG 2 cut(s) 679, 745
BmeT110I CYCGRG 1 cut(s) 823
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 3 cut(s) 78, 552, 676
BmrFI CCNGG 2 cut(s) 679, 745
BmsI GCATC 2 cut(s) 91, 657
BpiI GAAGAC 1 cut(s) 955
BpmI CTGGAG 1 cut(s) 745
Bpu1102I GCTNAGC 1 cut(s) 762
BsaI GGTCTC 1 cut(s) 1115
BsaJI CCNNGG 1 cut(s) 500
BsaWI WCCGGW 2 cut(s) 206, 547
BsaXI ACNNNNNCTCC 4 cut(s) 27, 57, 1010, 1040
Bsc4I CCNNNNNNNGG 1 cut(s) 744
Bse1I ACTGG 3 cut(s) 655, 722, 728
BseBI CCWGG 2 cut(s) 679, 745
BseDI CCNNGG 1 cut(s) 500
BseGI GGATG 4 cut(s) 106, 672, 1016, 1020
BseLI CCNNNNNNNGG 1 cut(s) 744
BseMII CTCAG 2 cut(s) 753, 781
BseNI ACTGG 3 cut(s) 655, 722, 728
BseSI GKGCMC 1 cut(s) 555
BseYI CCCAGC 2 cut(s) 126, 670
BshFI GGCC 3 cut(s) 70, 621, 1138
BshNI GGYRCC 2 cut(s) 76, 550
BsiHKAI GWGCWC 1 cut(s) 768
BsiHKCI CYCGRG 1 cut(s) 823
BsiSI CCGG 2 cut(s) 207, 548
BslFI GGGAC 1 cut(s) 805
BslI CCNNNNNNNGG 1 cut(s) 744
BsmAI GTCTC 2 cut(s) 593, 1115
BsmFI GGGAC 1 cut(s) 805
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 3 cut(s) 70, 621, 1138
Bso31I GGTCTC 1 cut(s) 1115
BsoBI CYCGRG 1 cut(s) 823
Bsp1286I GDGCHC 2 cut(s) 555, 768
Bsp1407I TGTACA 1 cut(s) 51
Bsp1720I GCTNAGC 1 cut(s) 762
BspACI CCGC 3 cut(s) 80, 441, 583
BspANI GGCC 3 cut(s) 70, 621, 1138
BspCNI CTCAG 2 cut(s) 754, 780
BspHI TCATGA 2 cut(s) 118, 886
BspLI GGNNCC 3 cut(s) 78, 552, 676
BspMI ACCTGC 1 cut(s) 122
BspPI GGATC 4 cut(s) 513, 539, 805, 1090
BspT107I GGYRCC 2 cut(s) 76, 550
BspTI CTTAAG 1 cut(s) 785
BspTNI GGTCTC 1 cut(s) 1115
BsrGI TGTACA 1 cut(s) 51
BsrI ACTGG 3 cut(s) 655, 722, 728
BssECI CCNNGG 1 cut(s) 500
BssT1I CCWWGG 1 cut(s) 500
Bst2UI CCWGG 2 cut(s) 679, 745
BstAFI CTTAAG 1 cut(s) 785
BstAPI GCANNNNNTGC 1 cut(s) 371
BstAUI TGTACA 1 cut(s) 51
BstC8I GCNNGC 3 cut(s) 84, 423, 905
BstDEI CTNAG 2 cut(s) 762, 767
BstF5I GGATG 4 cut(s) 106, 672, 1016, 1020
BstMAI GTCTC 2 cut(s) 593, 1115
BstMWI GCNNNNNNNGC 6 cut(s) 76, 371, 627, 900, 913, 989
BstNI CCWGG 2 cut(s) 679, 745
BstSCI CCNGG 2 cut(s) 677, 743
BstSFI CTRYAG 2 cut(s) 801, 1062
BstSLI GKGCMC 1 cut(s) 555
BstV2I GAAGAC 1 cut(s) 955
BstX2I RGATCY 2 cut(s) 797, 1082
BstXI CCANNNNNNTGG 1 cut(s) 717
BstYI RGATCY 2 cut(s) 797, 1082
BsuRI GGCC 3 cut(s) 70, 621, 1138
BtsCI GGATG 4 cut(s) 106, 672, 1016, 1020
BveI ACCTGC 1 cut(s) 122
Cac8I GCNNGC 3 cut(s) 84, 423, 905
CaiI CAGNNNCTG 1 cut(s) 591
CciI TCATGA 2 cut(s) 118, 886
Cfr13I GGNCC 1 cut(s) 68
Csp6I GTAC 2 cut(s) 52, 734
CviAII CATG 3 cut(s) 119, 323, 887
CviQI GTAC 2 cut(s) 52, 734
DdeI CTNAG 2 cut(s) 762, 767
Ecl136II GAGCTC 1 cut(s) 766
Eco130I CCWWGG 1 cut(s) 500
Eco147I AGGCCT 1 cut(s) 1138
Eco24I GRGCYC 1 cut(s) 768
Eco31I GGTCTC 1 cut(s) 1115
Eco53kI GAGCTC 1 cut(s) 766
Eco88I CYCGRG 1 cut(s) 823
EcoICRI GAGCTC 1 cut(s) 766
EcoO109I RGGNCCY 1 cut(s) 68
EcoRII CCWGG 2 cut(s) 677, 743
EcoT14I CCWWGG 1 cut(s) 500
EcoT38I GRGCYC 1 cut(s) 768
ErhI CCWWGG 1 cut(s) 500
FaeI CATG 3 cut(s) 122, 326, 890
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 1 cut(s) 805
FatI CATG 3 cut(s) 118, 322, 886
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 80
FokI GGATG 4 cut(s) 113, 679, 1023, 1027
FriOI GRGCYC 1 cut(s) 768
Fsp4HI GCNGC 1 cut(s) 80
FspBI CTAG 6 cut(s) 212, 245, 455, 513, 1076, 1140
GluI GCNGC 1 cut(s) 80
GsaI CCCAGC 2 cut(s) 130, 674
GsuI CTGGAG 1 cut(s) 745
HaeIII GGCC 3 cut(s) 70, 621, 1138
HapII CCGG 2 cut(s) 207, 548
Hin1II CATG 3 cut(s) 122, 326, 890
HinfI GANTC 2 cut(s) 115, 740
HpaII CCGG 2 cut(s) 207, 548
HphI GGTGA 3 cut(s) 241, 286, 1043
Hpy166II GTNNAC 1 cut(s) 392
Hpy188I TCNGA 4 cut(s) 345, 574, 770, 1087
Hpy188III TCNNGA 8 cut(s) 119, 173, 534, 710, 873, 887, 967, 1076
Hpy8I GTNNAC 1 cut(s) 392
HpyAV CCTTC 3 cut(s) 611, 990, 1115
HpyCH4IV ACGT 1 cut(s) 188
HpyCH4V TGCA 5 cut(s) 8, 104, 143, 365, 374
HpyF10VI GCNNNNNNNGC 6 cut(s) 76, 371, 627, 900, 913, 989
HpyF3I CTNAG 2 cut(s) 762, 767
HpySE526I ACGT 1 cut(s) 188
Hsp92II CATG 3 cut(s) 122, 326, 890
Ksp22I TGATCA 1 cut(s) 159
LmnI GCTCC 2 cut(s) 35, 674
LweI GCATC 2 cut(s) 91, 657
MaeI CTAG 6 cut(s) 212, 245, 455, 513, 1076, 1140
MaeII ACGT 1 cut(s) 188
MboII GAAGA 3 cut(s) 598, 955, 1061
MflI RGATCY 2 cut(s) 797, 1082
MhlI GDGCHC 2 cut(s) 555, 768
MluCI AATT 6 cut(s) 55, 92, 138, 152, 176, 705
MnlI CCTC 6 cut(s) 59, 105, 574, 587, 1072, 1100
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 4 cut(s) 95, 183, 786, 924
MslI CAYNNNNRTG 1 cut(s) 957
MspCI CTTAAG 1 cut(s) 785
MspI CCGG 2 cut(s) 207, 548
MspR9I CCNGG 2 cut(s) 679, 745
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 2 cut(s) 679, 745
MwoI GCNNNNNNNGC 6 cut(s) 76, 371, 627, 900, 913, 989
NlaIII CATG 3 cut(s) 122, 326, 890
NlaIV GGNNCC 3 cut(s) 78, 552, 676
PagI TCATGA 2 cut(s) 118, 886
PceI AGGCCT 1 cut(s) 1138
PctI GAATGC 1 cut(s) 10
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 2 cut(s) 115, 740
PkrI GCNGC 1 cut(s) 81
Psp124BI GAGCTC 1 cut(s) 768
Psp6I CCWGG 2 cut(s) 677, 743
PspFI CCCAGC 2 cut(s) 126, 670
PspGI CCWGG 2 cut(s) 677, 743
PspN4I GGNNCC 3 cut(s) 78, 552, 676
PspPI GGNCC 1 cut(s) 68
PstNI CAGNNNCTG 1 cut(s) 591
PsuI RGATCY 2 cut(s) 797, 1082
RsaI GTAC 2 cut(s) 53, 735
RsaNI GTAC 2 cut(s) 52, 734
RseI CAYNNNNRTG 1 cut(s) 957
SacI GAGCTC 1 cut(s) 768
SaqAI TTAA 4 cut(s) 95, 183, 786, 924
SatI GCNGC 1 cut(s) 80
Sau96I GGNCC 1 cut(s) 68
ScrFI CCNGG 2 cut(s) 679, 745
SduI GDGCHC 2 cut(s) 555, 768
SfaNI GCATC 2 cut(s) 91, 657
SfcI CTRYAG 2 cut(s) 801, 1062
SmiMI CAYNNNNRTG 1 cut(s) 957
SmlI CTYRAG 1 cut(s) 785
SmoI CTYRAG 1 cut(s) 785
Sse9I AATT 6 cut(s) 55, 92, 138, 152, 176, 705
SseBI AGGCCT 1 cut(s) 1138
SsiI CCGC 3 cut(s) 80, 441, 583
SspI AATATT 1 cut(s) 928
SspMI CTAG 6 cut(s) 212, 245, 455, 513, 1076, 1140
SstI GAGCTC 1 cut(s) 768
StuI AGGCCT 1 cut(s) 1138
StyD4I CCNGG 2 cut(s) 677, 743
StyI CCWWGG 1 cut(s) 500
TaiI ACGT 1 cut(s) 191
TaqI TCGA 4 cut(s) 174, 476, 874, 994
TasI AATT 6 cut(s) 55, 92, 138, 152, 176, 705
TatI WGTACW 1 cut(s) 51
TauI GCSGC 1 cut(s) 82
TfiI GAWTC 2 cut(s) 115, 740
Tru1I TTAA 4 cut(s) 95, 183, 786, 924
Tru9I TTAA 4 cut(s) 95, 183, 786, 924
TspDTI ATGAA 5 cut(s) 17, 107, 135, 875, 903
TspGWI ACGGA 2 cut(s) 558, 1023
Vha464I CTTAAG 1 cut(s) 785
XapI RAATTY 1 cut(s) 705
XbaI TCTAGA 1 cut(s) 1075
XmnI GAANNNNTTC 1 cut(s) 18
XspI CTAG 6 cut(s) 212, 245, 455, 513, 1076, 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.