FvH4_1g14390

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0016731
Physical Location & Seq
Reverse (-)
8028010 .. 8029102
1093 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14390.t1

Sequence Viewer

Length: 381 bp
ATGGGCGTCTGCGCTTCGTCTCCGACCATCAAGCTCCAGAAAAATGGAGGCAGAAGCAGTCGCAGACCTGACGGAACAAGGTCGGACTTCATCGGACGTCCAAAAGGGGTCGTCGTGATCGACGTTGACGGTGATCGGGTGGAAGAGTTGAAGCAGCCAACCCAAGCCAGGCGGATCATTTCGCAGCATCCCGACCATGTTCTCTGCAACTCCGAGGCCTTGTCCGTCGGCACGTGCGCGCCTCACGTGGCCGACGACGAGGAGCTTCAGACGGGTCATATTTACTTTCTCATGCCACTCCAACAGGCCCAGAATCCGCTTTCTCTACCGGAACTTTGTAATCTCGCGATCAAGGCCAGTTCTGCATTAGGGAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

127

Amino Acids

13.64

Weight (kDa)

7.72

Isoelectric Point (pI)

61.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 34 - 124 1e-20 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016731)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14390
malus_domestica MD15G1272000.v1.1
pyrus_communis pycom15g23590
rosa_chinensis RchiOBHm_Chr2g0103101
rosa_laevigata RLG00000017207
rosa_multiflora Rmu_co8158798.1_g000001 Rmu_sc0000388.1_g000009
rosa_roxburghii Rroxscaffold_2G00139930
rosa_rugosa Rorug02G0112000
rosa_samantha Rh2AG160400 Rh2BG166900 Rh2CG167500 Rh2DG165900
rosa_wichuraiana Rw2G012560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 100
AccII CGCG 2 cut(s) 239, 347
AciI CCGC 2 cut(s) 172, 317
AclWI GGATC 1 cut(s) 182
AcoI YGGCCR 1 cut(s) 249
AcuI CTGAAG 1 cut(s) 251
AcvI CACGTG 2 cut(s) 234, 247
AcyI GRCGYC 2 cut(s) 6, 97
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 1 cut(s) 151
AjnI CCWGG 1 cut(s) 167
AluBI AGCT 2 cut(s) 34, 265
AluI AGCT 2 cut(s) 34, 265
Alw26I GTCTC 1 cut(s) 24
AlwI GGATC 1 cut(s) 182
AoxI GGCC 4 cut(s) 216, 249, 306, 354
ApeKI GCWGC 2 cut(s) 154, 184
AspLEI GCGC 3 cut(s) 14, 239, 241
AspS9I GGNCC 1 cut(s) 307
AsuHPI GGTGA 1 cut(s) 143
BbrPI CACGTG 2 cut(s) 234, 247
BbvI GCAGC 2 cut(s) 166, 196
BccI CCATC 1 cut(s) 35
BciT130I CCWGG 1 cut(s) 169
BcoDI GTCTC 1 cut(s) 24
BisI GCNGC 2 cut(s) 155, 185
BlsI GCNGC 2 cut(s) 156, 186
Bme1390I CCNGG 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 307
BmrFI CCNGG 1 cut(s) 169
BmsI GCATC 1 cut(s) 196
BpmI CTGGAG 1 cut(s) 20
BsaAI YACGTR 2 cut(s) 234, 247
BsaHI GRCGYC 2 cut(s) 6, 97
BsaJI CCNNGG 1 cut(s) 213
BsaWI WCCGGW 1 cut(s) 328
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse1I ACTGG 1 cut(s) 357
BseBI CCWGG 1 cut(s) 169
BseDI CCNNGG 1 cut(s) 213
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 357
BsePI GCGCGC 1 cut(s) 237
BseRI GAGGAG 1 cut(s) 275
BseXI GCAGC 2 cut(s) 166, 196
Bsh1236I CGCG 2 cut(s) 239, 347
BshFI GGCC 4 cut(s) 218, 251, 308, 356
BsiSI CCGG 1 cut(s) 329
BslI CCNNNNNNNGG 1 cut(s) 168
BsmAI GTCTC 1 cut(s) 24
BsmBI CGTCTC 1 cut(s) 24
BsnI GGCC 4 cut(s) 218, 251, 308, 356
Bsp143I GATC 4 cut(s) 117, 133, 174, 348
Bsp68I TCGCGA 1 cut(s) 347
BspACI CCGC 2 cut(s) 172, 317
BspANI GGCC 4 cut(s) 218, 251, 308, 356
BspFNI CGCG 2 cut(s) 239, 347
BspPI GGATC 1 cut(s) 182
BsrI ACTGG 1 cut(s) 357
BssECI CCNNGG 1 cut(s) 213
BssHII GCGCGC 1 cut(s) 237
BssMI GATC 4 cut(s) 117, 133, 174, 348
BssNI GRCGYC 2 cut(s) 6, 97
Bst2UI CCWGG 1 cut(s) 169
Bst4CI ACNGT 1 cut(s) 131
Bst6I CTCTTC 1 cut(s) 138
BstACI GRCGYC 2 cut(s) 6, 97
BstBAI YACGTR 2 cut(s) 234, 247
BstC8I GCNNGC 1 cut(s) 239
BstF5I GGATG 1 cut(s) 187
BstFNI CGCG 2 cut(s) 239, 347
BstHHI GCGC 3 cut(s) 14, 239, 241
BstKTI GATC 4 cut(s) 120, 136, 177, 351
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 4 cut(s) 117, 133, 174, 348
BstMWI GCNNNNNNNGC 2 cut(s) 353, 362
BstNI CCWGG 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 167
BstUI CGCG 2 cut(s) 239, 347
BstV1I GCAGC 2 cut(s) 166, 196
BstXI CCANNNNNNTGG 1 cut(s) 44
BsuRI GGCC 4 cut(s) 218, 251, 308, 356
BtsCI GGATG 1 cut(s) 187
BtuMI TCGCGA 1 cut(s) 347
Cac8I GCNNGC 1 cut(s) 239
CfoI GCGC 3 cut(s) 14, 239, 241
Cfr13I GGNCC 1 cut(s) 307
CviAII CATG 2 cut(s) 197, 292
CviJI RGCY 8 cut(s) 34, 157, 167, 218, 251, 265, 308, 356
CviKI_1 RGCY 8 cut(s) 34, 157, 167, 218, 251, 265, 308, 356
DpnI GATC 4 cut(s) 119, 135, 176, 350
DpnII GATC 4 cut(s) 117, 133, 174, 348
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
EciI GGCGGA 1 cut(s) 187
Eco147I AGGCCT 1 cut(s) 218
Eco57I CTGAAG 1 cut(s) 251
Eco72I CACGTG 2 cut(s) 234, 247
EcoRII CCWGG 1 cut(s) 167
Esp3I CGTCTC 1 cut(s) 24
FaeI CATG 2 cut(s) 200, 295
FaiI YATR 3 cut(s) 198, 279, 293
FatI CATG 2 cut(s) 196, 291
Fnu4HI GCNGC 2 cut(s) 155, 185
FokI GGATG 1 cut(s) 174
Fsp4HI GCNGC 2 cut(s) 155, 185
GlaI GCGC 3 cut(s) 13, 238, 240
GluI GCNGC 2 cut(s) 155, 185
GsuI CTGGAG 1 cut(s) 20
HaeIII GGCC 4 cut(s) 218, 251, 308, 356
HapII CCGG 1 cut(s) 329
HhaI GCGC 3 cut(s) 14, 239, 241
Hin1I GRCGYC 2 cut(s) 6, 97
Hin1II CATG 2 cut(s) 200, 295
Hin6I GCGC 3 cut(s) 12, 237, 239
HinP1I GCGC 3 cut(s) 12, 237, 239
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 1 cut(s) 313
HpaII CCGG 1 cut(s) 329
HphI GGTGA 1 cut(s) 143
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 5 cut(s) 24, 85, 95, 214, 270
Hpy188III TCNNGA 4 cut(s) 37, 115, 191, 346
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 5 cut(s) 116, 125, 230, 257, 260
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4IV ACGT 4 cut(s) 97, 123, 233, 246
HpyCH4V TGCA 2 cut(s) 207, 365
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 362
HpySE526I ACGT 4 cut(s) 97, 123, 233, 246
Hsp92I GRCGYC 2 cut(s) 6, 97
Hsp92II CATG 2 cut(s) 200, 295
HspAI GCGC 3 cut(s) 12, 237, 239
Kzo9I GATC 4 cut(s) 117, 133, 174, 348
LmnI GCTCC 2 cut(s) 39, 262
LpnPI CCDG 8 cut(s) 50, 81, 154, 181, 290, 323, 342, 370
Lsp1109I GCAGC 2 cut(s) 166, 196
LweI GCATC 1 cut(s) 196
MaeII ACGT 4 cut(s) 97, 123, 233, 246
MalI GATC 4 cut(s) 119, 135, 176, 350
MboI GATC 4 cut(s) 117, 133, 174, 348
MboII GAAGA 1 cut(s) 155
MmeI TCCRAC 3 cut(s) 47, 63, 325
MnlI CCTC 4 cut(s) 41, 208, 252, 253
MspI CCGG 1 cut(s) 329
MspR9I CCNGG 1 cut(s) 169
MvaI CCWGG 1 cut(s) 169
MvnI CGCG 2 cut(s) 239, 347
MwoI GCNNNNNNNGC 2 cut(s) 353, 362
NdeII GATC 4 cut(s) 117, 133, 174, 348
NlaIII CATG 2 cut(s) 200, 295
NruI TCGCGA 1 cut(s) 347
PauI GCGCGC 1 cut(s) 237
PceI AGGCCT 1 cut(s) 218
PcsI WCGNNNNNNNCGW 3 cut(s) 117, 120, 252
PfeI GAWTC 1 cut(s) 313
PkrI GCNGC 2 cut(s) 156, 186
PmaCI CACGTG 2 cut(s) 234, 247
PmlI CACGTG 2 cut(s) 234, 247
Ppu21I YACGTR 2 cut(s) 234, 247
Psp6I CCWGG 1 cut(s) 167
PspCI CACGTG 2 cut(s) 234, 247
PspGI CCWGG 1 cut(s) 167
PspPI GGNCC 1 cut(s) 307
PteI GCGCGC 1 cut(s) 237
RruI TCGCGA 1 cut(s) 347
SatI GCNGC 2 cut(s) 155, 185
Sau3AI GATC 4 cut(s) 117, 133, 174, 348
Sau96I GGNCC 1 cut(s) 307
ScrFI CCNGG 1 cut(s) 169
SetI ASST 8 cut(s) 36, 70, 83, 100, 126, 236, 249, 267
SfaNI GCATC 1 cut(s) 196
SseBI AGGCCT 1 cut(s) 218
SsiI CCGC 2 cut(s) 172, 317
StuI AGGCCT 1 cut(s) 218
StyD4I CCNGG 1 cut(s) 167
TaaI ACNGT 1 cut(s) 131
TaiI ACGT 4 cut(s) 100, 126, 236, 249
TaqI TCGA 1 cut(s) 120
TfiI GAWTC 1 cut(s) 313
TseI GCWGC 2 cut(s) 154, 184
TspDTI ATGAA 1 cut(s) 79
TspGWI ACGGA 2 cut(s) 87, 214
ZraI GACGTC 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.