MD15G1272000.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016731
Physical Location & Seq
Reverse (-)
23744210 .. 23744662
453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1272000.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGGCGTCTGTGCTTCCACTCCGACCAGCACAAAAAGCAGAGGAGGAGGAGCACCGCGACCAGATGCGATCGGACGGCCGACAAGCAGCTTAGTGATCGACGCGGACGGTCGGATGCAAGAGTTGAAGCGACCAACCCAATCAAAGCACATCACTTCCCAGAATCCCAATCATTTCCTCTGCAACTCCGAAACCATGTCCATCGGCACGTGCTTGACCCACGTGCCCGACGAGGAGGAGCTCCAGCCGGGCCAAATTTACTTCCTGCTGCCACTGCGTCAGACCCAGAAGCCCCTCTCTCTGCCGGACCTCTGCGATCTGGCCATCAAGGCCAGCTCTGCGATGGCCATTGGCCAGAGACGGTGGTCGCCTAACTTCAAAGCTCAATTTTCCCTGAATTTTTATTTTTTGGATAATAAGTTTTTTATTTATTTTTGTGATCGAATGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

151

Amino Acids

16.77

Weight (kDa)

8.67

Isoelectric Point (pI)

55.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 17 - 117 5.3e-22 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016731)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14390
malus_domestica MD15G1272000.v1.1
pyrus_communis pycom15g23590
rosa_chinensis RchiOBHm_Chr2g0103101
rosa_laevigata RLG00000017207
rosa_multiflora Rmu_co8158798.1_g000001 Rmu_sc0000388.1_g000009
rosa_roxburghii Rroxscaffold_2G00139930
rosa_rugosa Rorug02G0112000
rosa_samantha Rh2AG160400 Rh2BG166900 Rh2CG167500 Rh2DG165900
rosa_wichuraiana Rw2G012560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 58, 104
AciI CCGC 2 cut(s) 56, 104
AcoI YGGCCR 4 cut(s) 77, 321, 345, 352
AcsI RAATTY 2 cut(s) 255, 397
AcvI CACGTG 2 cut(s) 210, 223
AcyI GRCGYC 1 cut(s) 6
AgsI TTSAA 2 cut(s) 127, 379
AluBI AGCT 4 cut(s) 90, 241, 336, 383
AluI AGCT 4 cut(s) 90, 241, 336, 383
Alw21I GWGCWC 2 cut(s) 55, 243
Alw26I GTCTC 1 cut(s) 352
AoxI GGCC 6 cut(s) 77, 250, 321, 330, 345, 352
ApeKI GCWGC 2 cut(s) 87, 268
ApoI RAATTY 2 cut(s) 255, 397
AspS9I GGNCC 2 cut(s) 250, 307
AsuC2I CCSGG 1 cut(s) 249
AvaII GGWCC 1 cut(s) 307
BaeGI GKGCMC 1 cut(s) 228
BalI TGGCCA 3 cut(s) 323, 347, 354
BanII GRGCYC 1 cut(s) 243
BbrPI CACGTG 2 cut(s) 210, 223
Bbv12I GWGCWC 2 cut(s) 55, 243
BbvI GCAGC 2 cut(s) 99, 255
BccI CCATC 3 cut(s) 209, 332, 337
BceAI ACGGC 1 cut(s) 92
BcnI CCSGG 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 352
BglI GCCNNNNNGGC 1 cut(s) 329
BisI GCNGC 2 cut(s) 88, 269
BlsI GCNGC 2 cut(s) 89, 270
Bme1390I CCNGG 1 cut(s) 249
Bme18I GGWCC 1 cut(s) 307
BmgT120I GGNCC 2 cut(s) 250, 307
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 2 cut(s) 55, 105
BoxI GACNNNNGTC 1 cut(s) 364
BpmI CTGGAG 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 249
BsaAI YACGTR 2 cut(s) 210, 223
BsaHI GRCGYC 1 cut(s) 6
BseGI GGATG 1 cut(s) 120
BseRI GAGGAG 5 cut(s) 57, 60, 63, 248, 251
BseSI GKGCMC 1 cut(s) 228
BseX3I CGGCCG 1 cut(s) 77
BseXI GCAGC 2 cut(s) 99, 255
Bsh1236I CGCG 2 cut(s) 58, 104
Bsh1285I CGRYCG 3 cut(s) 72, 80, 112
BshFI GGCC 6 cut(s) 79, 252, 323, 332, 347, 354
BsiEI CGRYCG 3 cut(s) 72, 80, 112
BsiHKAI GWGCWC 2 cut(s) 55, 243
BsiSI CCGG 2 cut(s) 248, 305
BsmAI GTCTC 1 cut(s) 352
BsmBI CGTCTC 1 cut(s) 352
BsmI GAATGC 1 cut(s) 450
BsnI GGCC 6 cut(s) 79, 252, 323, 332, 347, 354
Bsp1286I GDGCHC 3 cut(s) 55, 228, 243
Bsp143I GATC 4 cut(s) 69, 96, 316, 439
BspACI CCGC 2 cut(s) 56, 104
BspANI GGCC 6 cut(s) 79, 252, 323, 332, 347, 354
BspFNI CGCG 2 cut(s) 58, 104
BssMI GATC 4 cut(s) 69, 96, 316, 439
BssNI GRCGYC 1 cut(s) 6
Bst4CI ACNGT 2 cut(s) 110, 363
BstACI GRCGYC 1 cut(s) 6
BstBAI YACGTR 2 cut(s) 210, 223
BstC8I GCNNGC 1 cut(s) 334
BstDEI CTNAG 1 cut(s) 91
BstF5I GGATG 1 cut(s) 120
BstFNI CGCG 2 cut(s) 58, 104
BstKTI GATC 4 cut(s) 72, 99, 319, 442
BstMAI GTCTC 1 cut(s) 352
BstMBI GATC 4 cut(s) 69, 96, 316, 439
BstMCI CGRYCG 3 cut(s) 72, 80, 112
BstMWI GCNNNNNNNGC 4 cut(s) 36, 274, 329, 338
BstPAI GACNNNNGTC 1 cut(s) 364
BstSCI CCNGG 1 cut(s) 247
BstSLI GKGCMC 1 cut(s) 228
BstUI CGCG 2 cut(s) 58, 104
BstV1I GCAGC 2 cut(s) 99, 255
BstZI CGGCCG 1 cut(s) 77
BsuRI GGCC 6 cut(s) 79, 252, 323, 332, 347, 354
BtgZI GCGATG 1 cut(s) 356
BtsCI GGATG 1 cut(s) 120
BtsI GCAGTG 1 cut(s) 272
BtsIMutI CAGTG 1 cut(s) 272
Cac8I GCNNGC 1 cut(s) 334
Cfr13I GGNCC 2 cut(s) 250, 307
CseI GACGC 2 cut(s) 110, 266
CviAII CATG 1 cut(s) 196
DdeI CTNAG 1 cut(s) 91
DpnI GATC 4 cut(s) 71, 98, 318, 441
DpnII GATC 4 cut(s) 69, 96, 316, 439
EaeI YGGCCR 4 cut(s) 77, 321, 345, 352
EagI CGGCCG 1 cut(s) 77
Ecl136II GAGCTC 1 cut(s) 241
EclXI CGGCCG 1 cut(s) 77
Eco24I GRGCYC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 307
Eco52I CGGCCG 1 cut(s) 77
Eco53kI GAGCTC 1 cut(s) 241
Eco72I CACGTG 2 cut(s) 210, 223
EcoICRI GAGCTC 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 243
Esp3I CGTCTC 1 cut(s) 352
FaeI CATG 1 cut(s) 199
FaiI YATR 1 cut(s) 197
FatI CATG 1 cut(s) 195
Fnu4HI GCNGC 2 cut(s) 88, 269
FokI GGATG 1 cut(s) 127
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 2 cut(s) 88, 269
GluI GCNGC 2 cut(s) 88, 269
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 6 cut(s) 79, 252, 323, 332, 347, 354
HapII CCGG 2 cut(s) 248, 305
HgaI GACGC 2 cut(s) 110, 266
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 1 cut(s) 199
HinfI GANTC 1 cut(s) 163
HpaII CCGG 2 cut(s) 248, 305
Hpy188I TCNGA 5 cut(s) 24, 74, 114, 190, 282
Hpy99I CGWCG 2 cut(s) 104, 233
HpyCH4III ACNGT 2 cut(s) 110, 363
HpyCH4IV ACGT 2 cut(s) 209, 222
HpyCH4V TGCA 3 cut(s) 118, 183, 448
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 274, 329, 338
HpyF3I CTNAG 1 cut(s) 91
HpySE526I ACGT 2 cut(s) 209, 222
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 1 cut(s) 199
Kzo9I GATC 4 cut(s) 69, 96, 316, 439
LmnI GCTCC 3 cut(s) 50, 238, 246
Lsp1109I GCAGC 2 cut(s) 99, 255
LweI GCATC 2 cut(s) 55, 105
MaeII ACGT 2 cut(s) 209, 222
MalI GATC 4 cut(s) 71, 98, 318, 441
MboI GATC 4 cut(s) 69, 96, 316, 439
MhlI GDGCHC 3 cut(s) 55, 228, 243
MlsI TGGCCA 3 cut(s) 323, 347, 354
MluCI AATT 3 cut(s) 255, 386, 397
MluNI TGGCCA 3 cut(s) 323, 347, 354
MmeI TCCRAC 2 cut(s) 47, 92
MnlI CCTC 8 cut(s) 35, 38, 41, 188, 226, 229, 305, 320
Mox20I TGGCCA 3 cut(s) 323, 347, 354
MscI TGGCCA 3 cut(s) 323, 347, 354
Msp20I TGGCCA 3 cut(s) 323, 347, 354
MspI CCGG 2 cut(s) 248, 305
MspR9I CCNGG 1 cut(s) 249
Mva1269I GAATGC 1 cut(s) 450
MvnI CGCG 2 cut(s) 58, 104
MwoI GCNNNNNNNGC 4 cut(s) 36, 274, 329, 338
NciI CCSGG 1 cut(s) 249
NdeII GATC 4 cut(s) 69, 96, 316, 439
NlaIII CATG 1 cut(s) 199
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PctI GAATGC 1 cut(s) 450
PfeI GAWTC 1 cut(s) 163
PkrI GCNGC 2 cut(s) 89, 270
Ple19I CGATCG 1 cut(s) 72
PmaCI CACGTG 2 cut(s) 210, 223
PmlI CACGTG 2 cut(s) 210, 223
Ppu21I YACGTR 2 cut(s) 210, 223
PshAI GACNNNNGTC 1 cut(s) 364
Psp124BI GAGCTC 1 cut(s) 243
PspCI CACGTG 2 cut(s) 210, 223
PspPI GGNCC 2 cut(s) 250, 307
PvuI CGATCG 1 cut(s) 72
SacI GAGCTC 1 cut(s) 243
SatI GCNGC 2 cut(s) 88, 269
Sau3AI GATC 4 cut(s) 69, 96, 316, 439
Sau96I GGNCC 2 cut(s) 250, 307
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 3 cut(s) 55, 228, 243
SetI ASST 7 cut(s) 92, 212, 225, 243, 312, 338, 385
SfaNI GCATC 2 cut(s) 55, 105
SfiI GGCCNNNNNGGCC 1 cut(s) 329
SinI GGWCC 1 cut(s) 307
Sse9I AATT 3 cut(s) 255, 386, 397
SsiI CCGC 2 cut(s) 56, 104
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 2 cut(s) 110, 363
TaiI ACGT 2 cut(s) 212, 225
TaqI TCGA 2 cut(s) 99, 442
TasI AATT 3 cut(s) 255, 386, 397
TfiI GAWTC 1 cut(s) 163
TscAI CASTG 1 cut(s) 279
TseI GCWGC 2 cut(s) 87, 268
TspRI CASTG 1 cut(s) 279
VpaK11BI GGWCC 1 cut(s) 307
XapI RAATTY 2 cut(s) 255, 397
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.