pycom15g23590

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016731
Physical Location & Seq
Reverse (-)
17733661 .. 17734050
390 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g23590.1

Sequence Viewer

Length: 390 bp
ATGGGCGTCTGTGCTTCCACTCCGACCTGCACAAAAAGCAGAGGAGGAGGAGCACCGCGACCAGATGCGATCGGACGGCCGACAAGCAGCATAGTGATCGACGCGGACGGTCGGATGCAAGAGTTGAAGCGACCAACCCAATCAAAGCACATCACTTCCCAGAATCCGAATCATTTCCTCTGCAACTCCGAAACCATGTCCATCGGCACGTGCTTGACCCACGTGCCCGACGAGGAGGAGCTCCAGGCGGGCCAAATTTACTTCCTGCTGCCACTGCGTCAGACCCAGAAGCCCCTCTCTCTGCCGGACCTCTGCGATCTGGCCATCAAGGCCAGCTCTGCGCTGGCCAGAGACGGTGGTCGCCTAACTTCAAAGCTCAAATTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

130

Amino Acids

13.89

Weight (kDa)

8.69

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 118 9e-23 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016731)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14390
malus_domestica MD15G1272000.v1.1
pyrus_communis pycom15g23590
rosa_chinensis RchiOBHm_Chr2g0103101
rosa_laevigata RLG00000017207
rosa_multiflora Rmu_co8158798.1_g000001 Rmu_sc0000388.1_g000009
rosa_roxburghii Rroxscaffold_2G00139930
rosa_rugosa Rorug02G0112000
rosa_samantha Rh2AG160400 Rh2BG166900 Rh2CG167500 Rh2DG165900
rosa_wichuraiana Rw2G012560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 35
AccII CGCG 2 cut(s) 58, 104
AciI CCGC 3 cut(s) 56, 104, 248
AcoI YGGCCR 3 cut(s) 77, 321, 345
AcsI RAATTY 2 cut(s) 255, 380
AcvI CACGTG 2 cut(s) 210, 223
AcyI GRCGYC 1 cut(s) 6
AgsI TTSAA 2 cut(s) 127, 372
AjnI CCWGG 1 cut(s) 243
AluBI AGCT 3 cut(s) 241, 336, 376
AluI AGCT 3 cut(s) 241, 336, 376
Alw21I GWGCWC 2 cut(s) 55, 243
Alw26I GTCTC 1 cut(s) 345
AoxI GGCC 5 cut(s) 77, 250, 321, 330, 345
ApeKI GCWGC 2 cut(s) 87, 268
ApoI RAATTY 2 cut(s) 255, 380
Asp700I GAANNNNTTC 1 cut(s) 173
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 2 cut(s) 250, 307
AvaII GGWCC 1 cut(s) 307
BaeGI GKGCMC 1 cut(s) 228
BalI TGGCCA 2 cut(s) 323, 347
BanII GRGCYC 1 cut(s) 243
BbrPI CACGTG 2 cut(s) 210, 223
Bbv12I GWGCWC 2 cut(s) 55, 243
BbvI GCAGC 2 cut(s) 99, 255
BccI CCATC 2 cut(s) 209, 332
BceAI ACGGC 1 cut(s) 92
BcgI CGANNNNNNTGC 2 cut(s) 79, 113
BciT130I CCWGG 1 cut(s) 245
BcoDI GTCTC 1 cut(s) 345
BfuAI ACCTGC 1 cut(s) 35
BglI GCCNNNNNGGC 1 cut(s) 329
BisI GCNGC 2 cut(s) 88, 269
BlsI GCNGC 2 cut(s) 89, 270
Bme1390I CCNGG 1 cut(s) 245
Bme18I GGWCC 1 cut(s) 307
BmgT120I GGNCC 2 cut(s) 250, 307
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 2 cut(s) 55, 105
BoxI GACNNNNGTC 1 cut(s) 357
BpmI CTGGAG 1 cut(s) 227
BsaAI YACGTR 2 cut(s) 210, 223
BsaHI GRCGYC 1 cut(s) 6
BseBI CCWGG 1 cut(s) 245
BseGI GGATG 1 cut(s) 120
BseRI GAGGAG 5 cut(s) 57, 60, 63, 248, 251
BseSI GKGCMC 1 cut(s) 228
BseX3I CGGCCG 1 cut(s) 77
BseXI GCAGC 2 cut(s) 99, 255
BsgI GTGCAG 1 cut(s) 13
Bsh1236I CGCG 2 cut(s) 58, 104
Bsh1285I CGRYCG 3 cut(s) 72, 80, 112
BshFI GGCC 5 cut(s) 79, 252, 323, 332, 347
BsiEI CGRYCG 3 cut(s) 72, 80, 112
BsiHKAI GWGCWC 2 cut(s) 55, 243
BsiSI CCGG 1 cut(s) 305
BsmAI GTCTC 1 cut(s) 345
BsmBI CGTCTC 1 cut(s) 345
BsnI GGCC 5 cut(s) 79, 252, 323, 332, 347
Bsp1286I GDGCHC 3 cut(s) 55, 228, 243
Bsp143I GATC 3 cut(s) 69, 96, 316
BspACI CCGC 3 cut(s) 56, 104, 248
BspANI GGCC 5 cut(s) 79, 252, 323, 332, 347
BspFNI CGCG 2 cut(s) 58, 104
BspMI ACCTGC 1 cut(s) 35
BssMI GATC 3 cut(s) 69, 96, 316
BssNI GRCGYC 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 2 cut(s) 110, 356
BstACI GRCGYC 1 cut(s) 6
BstBAI YACGTR 2 cut(s) 210, 223
BstC8I GCNNGC 3 cut(s) 250, 334, 345
BstF5I GGATG 1 cut(s) 120
BstFNI CGCG 2 cut(s) 58, 104
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 3 cut(s) 72, 99, 319
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 3 cut(s) 69, 96, 316
BstMCI CGRYCG 3 cut(s) 72, 80, 112
BstMWI GCNNNNNNNGC 4 cut(s) 36, 274, 329, 338
BstNI CCWGG 1 cut(s) 245
BstPAI GACNNNNGTC 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 243
BstSLI GKGCMC 1 cut(s) 228
BstUI CGCG 2 cut(s) 58, 104
BstV1I GCAGC 2 cut(s) 99, 255
BstZI CGGCCG 1 cut(s) 77
BsuRI GGCC 5 cut(s) 79, 252, 323, 332, 347
BtsCI GGATG 1 cut(s) 120
BtsI GCAGTG 1 cut(s) 272
BtsIMutI CAGTG 1 cut(s) 272
BveI ACCTGC 1 cut(s) 35
Cac8I GCNNGC 3 cut(s) 250, 334, 345
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 250, 307
CseI GACGC 2 cut(s) 110, 266
CviAII CATG 1 cut(s) 196
CviJI RGCY 9 cut(s) 79, 241, 252, 292, 323, 332, 336, 347, 376
CviKI_1 RGCY 9 cut(s) 79, 241, 252, 292, 323, 332, 336, 347, 376
DpnI GATC 3 cut(s) 71, 98, 318
DpnII GATC 3 cut(s) 69, 96, 316
EaeI YGGCCR 3 cut(s) 77, 321, 345
EagI CGGCCG 1 cut(s) 77
Ecl136II GAGCTC 1 cut(s) 241
EclXI CGGCCG 1 cut(s) 77
Eco24I GRGCYC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 307
Eco52I CGGCCG 1 cut(s) 77
Eco53kI GAGCTC 1 cut(s) 241
Eco72I CACGTG 2 cut(s) 210, 223
EcoICRI GAGCTC 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 243
EcoT38I GRGCYC 1 cut(s) 243
Esp3I CGTCTC 1 cut(s) 345
FaeI CATG 1 cut(s) 199
FaiI YATR 2 cut(s) 92, 197
FatI CATG 1 cut(s) 195
FauI CCCGC 1 cut(s) 241
Fnu4HI GCNGC 2 cut(s) 88, 269
FokI GGATG 1 cut(s) 127
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 2 cut(s) 88, 269
GlaI GCGC 1 cut(s) 342
GluI GCNGC 2 cut(s) 88, 269
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 5 cut(s) 79, 252, 323, 332, 347
HapII CCGG 1 cut(s) 305
HgaI GACGC 2 cut(s) 110, 266
HhaI GCGC 1 cut(s) 343
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 1 cut(s) 199
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HinfI GANTC 2 cut(s) 163, 169
HpaII CCGG 1 cut(s) 305
Hpy188I TCNGA 6 cut(s) 24, 74, 114, 168, 190, 282
Hpy99I CGWCG 2 cut(s) 104, 233
HpyCH4III ACNGT 2 cut(s) 110, 356
HpyCH4IV ACGT 2 cut(s) 209, 222
HpyCH4V TGCA 3 cut(s) 30, 118, 183
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 274, 329, 338
HpySE526I ACGT 2 cut(s) 209, 222
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 1 cut(s) 199
HspAI GCGC 1 cut(s) 341
Kzo9I GATC 3 cut(s) 69, 96, 316
LmnI GCTCC 3 cut(s) 50, 238, 246
Lsp1109I GCAGC 2 cut(s) 99, 255
LweI GCATC 2 cut(s) 55, 105
MaeII ACGT 2 cut(s) 209, 222
MalI GATC 3 cut(s) 71, 98, 318
MboI GATC 3 cut(s) 69, 96, 316
MhlI GDGCHC 3 cut(s) 55, 228, 243
MlsI TGGCCA 2 cut(s) 323, 347
MluCI AATT 2 cut(s) 255, 380
MluNI TGGCCA 2 cut(s) 323, 347
MmeI TCCRAC 2 cut(s) 47, 92
MnlI CCTC 8 cut(s) 35, 38, 41, 188, 226, 229, 305, 320
Mox20I TGGCCA 2 cut(s) 323, 347
MroXI GAANNNNTTC 1 cut(s) 173
MscI TGGCCA 2 cut(s) 323, 347
Msp20I TGGCCA 2 cut(s) 323, 347
MspI CCGG 1 cut(s) 305
MspR9I CCNGG 1 cut(s) 245
MvaI CCWGG 1 cut(s) 245
MvnI CGCG 2 cut(s) 58, 104
MwoI GCNNNNNNNGC 4 cut(s) 36, 274, 329, 338
NdeII GATC 3 cut(s) 69, 96, 316
NlaIII CATG 1 cut(s) 199
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 173
PfeI GAWTC 2 cut(s) 163, 169
PkrI GCNGC 2 cut(s) 89, 270
Ple19I CGATCG 1 cut(s) 72
PmaCI CACGTG 2 cut(s) 210, 223
PmlI CACGTG 2 cut(s) 210, 223
Ppu21I YACGTR 2 cut(s) 210, 223
PshAI GACNNNNGTC 1 cut(s) 357
Psp124BI GAGCTC 1 cut(s) 243
Psp6I CCWGG 1 cut(s) 243
PspCI CACGTG 2 cut(s) 210, 223
PspGI CCWGG 1 cut(s) 243
PspPI GGNCC 2 cut(s) 250, 307
PvuI CGATCG 1 cut(s) 72
SacI GAGCTC 1 cut(s) 243
SatI GCNGC 2 cut(s) 88, 269
Sau3AI GATC 3 cut(s) 69, 96, 316
Sau96I GGNCC 2 cut(s) 250, 307
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 3 cut(s) 55, 228, 243
SetI ASST 7 cut(s) 29, 212, 225, 243, 312, 338, 378
SfaNI GCATC 2 cut(s) 55, 105
SfiI GGCCNNNNNGGCC 1 cut(s) 329
SinI GGWCC 1 cut(s) 307
Sse9I AATT 2 cut(s) 255, 380
SsiI CCGC 3 cut(s) 56, 104, 248
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 243
TaaI ACNGT 2 cut(s) 110, 356
TaiI ACGT 2 cut(s) 212, 225
TaqI TCGA 1 cut(s) 99
TasI AATT 2 cut(s) 255, 380
TfiI GAWTC 2 cut(s) 163, 169
TscAI CASTG 1 cut(s) 279
TseI GCWGC 2 cut(s) 87, 268
TspRI CASTG 1 cut(s) 279
VpaK11BI GGWCC 1 cut(s) 307
XapI RAATTY 2 cut(s) 255, 380
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XmnI GAANNNNTTC 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.