Rh2BG166900

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0016731
Physical Location & Seq
Reverse (-)
14721875 .. 14723327
1453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG166900.1

Sequence Viewer

Length: 381 bp
ATGGGCGTCTGCGCTTCGTCTCCAACCATCAAGCTCAAGAGAAATGGAGGCAGAAGCAGTAGCAGACAAGAAGGAACAAGCTCGGACTACATCGGACGTCCAAAAGGGATCGTCGTGATCGACGTGGACGGTGATCAGGTGCAAGAGTTGAAGCAACCGACCCAAGCCAGGGGGATCATTTCGCAGTATCCCAACCATGTTCTCTGCAACTCCGAAGCCATGTCCGTCGGCACGTGCGCGCCTCACGTGCCCGACGACGAGGAGCTCCAACCGGGTCAAATTTATTTTCTGGTGCCACTCCGCCAGGCCCAGAATCCGCTTTCTCTACCGGAACTTTGTAATCTCGCAATAAAGGCCAGTTCTGCACTGGCGAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

126

Amino Acids

13.54

Weight (kDa)

7.67

Isoelectric Point (pI)

53.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 33 - 124 3e-21 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016731)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14390
malus_domestica MD15G1272000.v1.1
pyrus_communis pycom15g23590
rosa_chinensis RchiOBHm_Chr2g0103101
rosa_laevigata RLG00000017207
rosa_multiflora Rmu_co8158798.1_g000001 Rmu_sc0000388.1_g000009
rosa_roxburghii Rroxscaffold_2G00139930
rosa_rugosa Rorug02G0112000
rosa_samantha Rh2AG160400 Rh2BG166900 Rh2CG167500 Rh2DG165900
rosa_wichuraiana Rw2G012560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 100
AccB1I GGYRCC 1 cut(s) 292
AccII CGCG 1 cut(s) 239
AciI CCGC 2 cut(s) 301, 317
AclWI GGATC 2 cut(s) 116, 182
AcsI RAATTY 1 cut(s) 279
AcvI CACGTG 2 cut(s) 234, 247
AcyI GRCGYC 2 cut(s) 6, 97
AfiI CCNNNNNNNGG 2 cut(s) 168, 169
AgsI TTSAA 1 cut(s) 151
AjiI CACGTC 1 cut(s) 124
AjnI CCWGG 2 cut(s) 167, 303
AluBI AGCT 3 cut(s) 34, 81, 265
AluI AGCT 3 cut(s) 34, 81, 265
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 1 cut(s) 24
AlwI GGATC 2 cut(s) 116, 182
AoxI GGCC 2 cut(s) 306, 354
ApoI RAATTY 1 cut(s) 279
AspLEI GCGC 3 cut(s) 14, 239, 241
AspS9I GGNCC 1 cut(s) 307
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 252
BanI GGYRCC 1 cut(s) 292
BanII GRGCYC 1 cut(s) 267
BbrPI CACGTG 2 cut(s) 234, 247
Bbv12I GWGCWC 1 cut(s) 267
BccI CCATC 1 cut(s) 35
BciT130I CCWGG 2 cut(s) 169, 305
BciVI GTATCC 1 cut(s) 198
BclI TGATCA 1 cut(s) 133
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 1 cut(s) 24
BfuI GTATCC 1 cut(s) 198
Bme1390I CCNGG 3 cut(s) 169, 273, 305
BmgBI CACGTC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 307
BmiI GGNNCC 1 cut(s) 294
BmrFI CCNGG 3 cut(s) 169, 273, 305
BpuEI CTTGAG 1 cut(s) 20
BpuMI CCSGG 1 cut(s) 273
BsaAI YACGTR 2 cut(s) 234, 247
BsaHI GRCGYC 2 cut(s) 6, 97
BsaJI CCNNGG 1 cut(s) 168
BsaWI WCCGGW 1 cut(s) 328
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 169
Bse1I ACTGG 2 cut(s) 357, 372
BseBI CCWGG 2 cut(s) 169, 305
BseDI CCNNGG 1 cut(s) 168
BseLI CCNNNNNNNGG 2 cut(s) 168, 169
BseNI ACTGG 2 cut(s) 357, 372
BsePI GCGCGC 1 cut(s) 237
BseRI GAGGAG 1 cut(s) 275
BseSI GKGCMC 1 cut(s) 252
BsgI GTGCAG 1 cut(s) 348
Bsh1236I CGCG 1 cut(s) 239
BshFI GGCC 2 cut(s) 308, 356
BshNI GGYRCC 1 cut(s) 292
BsiHKAI GWGCWC 1 cut(s) 267
BsiSI CCGG 2 cut(s) 272, 329
BslI CCNNNNNNNGG 2 cut(s) 168, 169
BsmAI GTCTC 1 cut(s) 24
BsmBI CGTCTC 1 cut(s) 24
BsnI GGCC 2 cut(s) 308, 356
Bsp1286I GDGCHC 2 cut(s) 252, 267
Bsp143I GATC 4 cut(s) 108, 117, 133, 174
BspACI CCGC 2 cut(s) 301, 317
BspANI GGCC 2 cut(s) 308, 356
BspFNI CGCG 1 cut(s) 239
BspLI GGNNCC 1 cut(s) 294
BspPI GGATC 2 cut(s) 116, 182
BspT107I GGYRCC 1 cut(s) 292
BsrI ACTGG 2 cut(s) 357, 372
BssECI CCNNGG 1 cut(s) 168
BssHII GCGCGC 1 cut(s) 237
BssMI GATC 4 cut(s) 108, 117, 133, 174
BssNI GRCGYC 2 cut(s) 6, 97
Bst2UI CCWGG 2 cut(s) 169, 305
Bst4CI ACNGT 1 cut(s) 131
BstACI GRCGYC 2 cut(s) 6, 97
BstBAI YACGTR 2 cut(s) 234, 247
BstC8I GCNNGC 1 cut(s) 239
BstFNI CGCG 1 cut(s) 239
BstHHI GCGC 3 cut(s) 14, 239, 241
BstKTI GATC 4 cut(s) 111, 120, 136, 177
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 4 cut(s) 108, 117, 133, 174
BstMWI GCNNNNNNNGC 3 cut(s) 247, 353, 362
BstNI CCWGG 2 cut(s) 169, 305
BstSCI CCNGG 3 cut(s) 167, 271, 303
BstSLI GKGCMC 1 cut(s) 252
BstUI CGCG 1 cut(s) 239
BsuI GTATCC 1 cut(s) 198
BsuRI GGCC 2 cut(s) 308, 356
BtrI CACGTC 1 cut(s) 124
BtsIMutI CAGTG 1 cut(s) 365
Cac8I GCNNGC 1 cut(s) 239
CfoI GCGC 3 cut(s) 14, 239, 241
Cfr13I GGNCC 1 cut(s) 307
CviAII CATG 2 cut(s) 197, 220
CviJI RGCY 7 cut(s) 34, 81, 167, 218, 265, 308, 356
CviKI_1 RGCY 7 cut(s) 34, 81, 167, 218, 265, 308, 356
DpnI GATC 4 cut(s) 110, 119, 135, 176
DpnII GATC 4 cut(s) 108, 117, 133, 174
EciI GGCGGA 1 cut(s) 290
Ecl136II GAGCTC 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 267
Eco53kI GAGCTC 1 cut(s) 265
Eco72I CACGTG 2 cut(s) 234, 247
EcoICRI GAGCTC 1 cut(s) 265
EcoRII CCWGG 2 cut(s) 167, 303
EcoT38I GRGCYC 1 cut(s) 267
Esp3I CGTCTC 1 cut(s) 24
FaeI CATG 2 cut(s) 200, 223
FaiI YATR 2 cut(s) 198, 221
FatI CATG 2 cut(s) 196, 219
FbaI TGATCA 1 cut(s) 133
FriOI GRGCYC 1 cut(s) 267
GlaI GCGC 3 cut(s) 13, 238, 240
HaeIII GGCC 2 cut(s) 308, 356
HapII CCGG 2 cut(s) 272, 329
HhaI GCGC 3 cut(s) 14, 239, 241
Hin1I GRCGYC 2 cut(s) 6, 97
Hin1II CATG 2 cut(s) 200, 223
Hin6I GCGC 3 cut(s) 12, 237, 239
HinP1I GCGC 3 cut(s) 12, 237, 239
HinfI GANTC 1 cut(s) 313
HpaII CCGG 2 cut(s) 272, 329
HphI GGTGA 1 cut(s) 143
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 3 cut(s) 85, 95, 214
Hpy188III TCNNGA 2 cut(s) 37, 115
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 5 cut(s) 116, 125, 230, 257, 260
HpyAV CCTTC 1 cut(s) 65
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4IV ACGT 4 cut(s) 97, 123, 233, 246
HpyCH4V TGCA 3 cut(s) 142, 207, 365
HpyF10VI GCNNNNNNNGC 3 cut(s) 247, 353, 362
HpySE526I ACGT 4 cut(s) 97, 123, 233, 246
Hsp92I GRCGYC 2 cut(s) 6, 97
Hsp92II CATG 2 cut(s) 200, 223
HspAI GCGC 3 cut(s) 12, 237, 239
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 4 cut(s) 108, 117, 133, 174
LmnI GCTCC 2 cut(s) 262, 270
MaeII ACGT 4 cut(s) 97, 123, 233, 246
MalI GATC 4 cut(s) 110, 119, 135, 176
MboI GATC 4 cut(s) 108, 117, 133, 174
MhlI GDGCHC 2 cut(s) 252, 267
MluCI AATT 1 cut(s) 279
MmeI TCCRAC 2 cut(s) 47, 292
MnlI CCTC 3 cut(s) 41, 252, 253
MspI CCGG 2 cut(s) 272, 329
MspR9I CCNGG 3 cut(s) 169, 273, 305
MvaI CCWGG 2 cut(s) 169, 305
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 3 cut(s) 247, 353, 362
NciI CCSGG 1 cut(s) 273
NdeII GATC 4 cut(s) 108, 117, 133, 174
NlaIII CATG 2 cut(s) 200, 223
NlaIV GGNNCC 1 cut(s) 294
PauI GCGCGC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 3 cut(s) 117, 120, 252
PfeI GAWTC 1 cut(s) 313
PmaCI CACGTG 2 cut(s) 234, 247
PmlI CACGTG 2 cut(s) 234, 247
Ppu21I YACGTR 2 cut(s) 234, 247
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 2 cut(s) 167, 303
PspCI CACGTG 2 cut(s) 234, 247
PspGI CCWGG 2 cut(s) 167, 303
PspN4I GGNNCC 1 cut(s) 294
PspPI GGNCC 1 cut(s) 307
PteI GCGCGC 1 cut(s) 237
SacI GAGCTC 1 cut(s) 267
Sau3AI GATC 4 cut(s) 108, 117, 133, 174
Sau96I GGNCC 1 cut(s) 307
ScrFI CCNGG 3 cut(s) 169, 273, 305
SduI GDGCHC 2 cut(s) 252, 267
SetI ASST 8 cut(s) 36, 83, 100, 126, 141, 236, 249, 267
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
Sse9I AATT 1 cut(s) 279
SsiI CCGC 2 cut(s) 301, 317
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 3 cut(s) 167, 271, 303
TaaI ACNGT 1 cut(s) 131
TaiI ACGT 4 cut(s) 100, 126, 236, 249
TaqI TCGA 1 cut(s) 120
TasI AATT 1 cut(s) 279
TfiI GAWTC 1 cut(s) 313
TscAI CASTG 1 cut(s) 372
TspGWI ACGGA 1 cut(s) 214
TspRI CASTG 1 cut(s) 372
XapI RAATTY 1 cut(s) 279
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
ZraI GACGTC 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.