FvH4_1g14980

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010983
Physical Location & Seq
Reverse (-)
8412006 .. 8415378
3373 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14980.t2

Sequence Viewer

Length: 1614 bp
ATGAGGCCAACCTCTTTCCTGCGTTTTTTTCTTGGAACATTGGTTGGCTTGAGTGCTTTGTGCATCATCAATGCAGAAGACCCTTATAGATATTTTACATGGACAGTTACATACGGAACCATTTCTCCATTGGGTGTTCCTCAACAGGCTATACTTATTAACGGTCAATTTCCTGGCCCAACAATTGAGGCTGTGACTAATGACAACATAGTCGTTGATGTCATTAACAAGTTGGATGAACCTTTCCTCATTACATGGAATGGAATTAAACAGAGGAAGTCAGTATGGCAAGATGGAGTCCTCGGAACCAATTGCCCGATTCCTCCAAACACAAGCTGGAGATACAAGTTTCAAACAAAGGATCAGATTGGAACATTCAGTTACTTCCCTTCAACGAAATTTCACAGAGTTGCTGGTGGTTTTGGGGGTTTCAATGTTGCACACAGGTCTGTGATAGCAGTCCCATATCCTATCCCCACTGAAGAGTTTACCGTTCTTGTTAGTGATTGGTACAAGACCAGCCACAAGGCATTGCAGCAGAAATTGGATTCTGGCATTCCTCTTCCTCTCCCTGATGCTCTACTTATAAATGGACTTCATGGATCTGCAACCTTCTCTGGTCAAAGAGGAAAGACCTACAAATTCAGGATATCAAATGTGGGAATAGCAACTTCAATCAACTTCAGGATTCAGGGTCATACAATGACACTTGTTGAAGTTGAAGGAGCTCATACCTTGCAAGAGGTGTACGAATCACTTGACGTTCATCCAGGCCAATCTGTAGGTGTTTTGGTTACATTACATGGTTCAGTCAAGGACTATTTCATCGTGGCCTCTTCCCGATTCACAAAACCCATTCTCACAACCACTGGAATTCTTCGATATGCTGGTTCTAGCATCAAGGCAGCAGGGCCATTGCCTGTTGGTCCAACCTATCACATTCACTGGTCTATGAAGCAAGCCAGGACAATCAGATTGAATTTGACAGCAAATGCAGCCAGGCCTAACCCTCAGGGATCATTCCATTATGGTACCATTCCAATAGTGAGAACACTTGTTTTGGCCAATACTGCTGCTAAGATCGGTGGCAAGCTTCGCTATGCTGTAAACAAGATCTCCTATGTTGATCCAGTGACCCCCTTGAAGCTGGCTGACTGGTTTAACATCCCTGGAGTATTCAACTTAAACTCAATCAAGGACACACCTACTCCAGGCCCTGCAAGCTTAGGTGTATCGGTCATAGGAACTGCACTCCACGACTTCGTCGAAGTCGTATTCCAGAACACCGAACCCACACTACAATCTTGGCATCTTGATGGAAGTAGCTTCTACATTGTTGGATATGGTTCTGGAAATTGGACCCCAGATATGAGGAGACGCTACAATTTGGCAGATGCTGTACCAAGACACACAGTTCAGGTGTATCCTTATGGTTGGAGTGCTATGTTGGTGTCTTTGGACAACAAGGGAATGTGGAACTTGAGGTCTGCAATCTGGGCAAGAAGGTACTTGGGTCAGCAATTGTATATCAGAGTTTGGAACGATGAGAAAAGCTTCTTCACTGAGAATGACATCCCTCAAAATGCATTATTTTGTGGCAAGGCCCATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

538

Amino Acids

59.63

Weight (kDa)

9.49

Isoelectric Point (pI)

30.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 36 - 149 1.5e-40 Multicopper oxidase
Cu-oxidase PF00394 161 - 297 7.9e-39 Multicopper oxidase
Cu-oxidase_2 PF07731 379 - 517 5.7e-30 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010983)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23630 AT2G23630 AT4G37160 AT4G37160
fragaria_vesca FvH4_1g14980 FvH4_1g14980
malus_domestica MD02G1163600.v1.1
prunus_persica Prupe.7G140600_v2.0.a1 Prupe.7G140600_v2.0.a1
pyrus_communis pycom02g13000
rosa_chinensis RchiOBHm_Chr2g0104041
rosa_laevigata RLG00000017280
rosa_multiflora Rmu_sc0000193.1_g000006
rosa_roxburghii Rroxscaffold_2G00139180
rosa_rugosa Rorug02G0119000
rosa_samantha Rh2AG168100 Rh2BG175100 Rh2CG174000 Rh2DG173600
rosa_wichuraiana Rw2G013150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 587
AasI GACNNNNNNGTC 1 cut(s) 209
Acc65I GGTACC 1 cut(s) 1031
AccB1I GGYRCC 1 cut(s) 1031
AclWI GGATC 4 cut(s) 369, 610, 1024, 1121
AcoI YGGCCR 1 cut(s) 1062
AcsI RAATTY 4 cut(s) 398, 641, 873, 979
AcuI CTGAAG 2 cut(s) 501, 667
AfaI GTAC 5 cut(s) 512, 749, 1033, 1401, 1508
AfiI CCNNNNNNNGG 1 cut(s) 1211
AgsI TTSAA 9 cut(s) 353, 393, 433, 675, 716, 722, 979, 1144, 1180
AjnI CCWGG 6 cut(s) 172, 769, 962, 998, 1168, 1210
AloI GAACNNNNNNTCC 2 cut(s) 109, 141
AluBI AGCT 7 cut(s) 336, 728, 1093, 1147, 1224, 1326, 1554
AluI AGCT 7 cut(s) 336, 728, 1093, 1147, 1224, 1326, 1554
Alw21I GWGCWC 1 cut(s) 730
Alw26I GTCTC 1 cut(s) 1369
AlwI GGATC 4 cut(s) 369, 610, 1024, 1121
AlwNI CAGNNNCTG 2 cut(s) 1217, 1397
AoxI GGCC 9 cut(s) 5, 175, 772, 831, 911, 1001, 1062, 1213, 1602
ApeKI GCWGC 4 cut(s) 535, 905, 995, 1073
ApoI RAATTY 4 cut(s) 398, 641, 873, 979
Asp700I GAANNNNTTC 2 cut(s) 121, 1553
Asp718I GGTACC 1 cut(s) 1031
AspS9I GGNCC 6 cut(s) 176, 911, 926, 1214, 1359, 1603
AvaII GGWCC 2 cut(s) 926, 1359
AxyI CCTNAGG 1 cut(s) 1011
BaeI ACNNNNGTAYC 2 cut(s) 1498, 1531
BalI TGGCCA 1 cut(s) 1064
BanI GGYRCC 1 cut(s) 1031
BanII GRGCYC 1 cut(s) 730
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 730
BbvI GCAGC 4 cut(s) 547, 917, 1007, 1060
BccI CCATC 2 cut(s) 287, 1310
BciT130I CCWGG 6 cut(s) 174, 771, 964, 1000, 1170, 1212
BciVI GTATCC 1 cut(s) 1434
BcoDI GTCTC 1 cut(s) 1369
BfaI CTAG 1 cut(s) 894
BfmI CTRYAG 1 cut(s) 780
BfuI GTATCC 1 cut(s) 1434
BglII AGATCT 1 cut(s) 1113
BisI GCNGC 4 cut(s) 536, 906, 996, 1074
BlsI GCNGC 4 cut(s) 537, 907, 997, 1075
Bme1390I CCNGG 6 cut(s) 174, 771, 964, 1000, 1170, 1212
Bme18I GGWCC 2 cut(s) 926, 1359
BmgT120I GGNCC 6 cut(s) 176, 911, 926, 1214, 1359, 1603
BmiI GGNNCC 4 cut(s) 118, 307, 1033, 1361
BmrFI CCNGG 6 cut(s) 174, 771, 964, 1000, 1170, 1212
BmsI GCATC 5 cut(s) 72, 565, 906, 1318, 1384
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 3 cut(s) 358, 1191, 1194
Bpu10I CCTNAGC 1 cut(s) 1225
BpuEI CTTGAG 2 cut(s) 70, 1501
BsaJI CCNNGG 2 cut(s) 301, 1168
BsaXI ACNNNNNCTCC 6 cut(s) 109, 139, 1100, 1130, 1192, 1222
Bsc4I CCNNNNNNNGG 1 cut(s) 1211
Bse1I ACTGG 4 cut(s) 874, 950, 1130, 1160
Bse21I CCTNAGG 1 cut(s) 1011
Bse3DI GCAATG 2 cut(s) 530, 914
BseBI CCWGG 6 cut(s) 174, 771, 964, 1000, 1170, 1212
BseDI CCNNGG 2 cut(s) 301, 1168
BseGI GGATG 4 cut(s) 241, 766, 1164, 1572
BseLI CCNNNNNNNGG 1 cut(s) 1211
BseMI GCAATG 2 cut(s) 530, 914
BseMII CTCAG 2 cut(s) 1025, 1554
BseNI ACTGG 4 cut(s) 874, 950, 1130, 1160
BseRI GAGGAG 1 cut(s) 1387
BseXI GCAGC 4 cut(s) 547, 917, 1007, 1060
BsgI GTGCAG 1 cut(s) 1233
BshFI GGCC 9 cut(s) 7, 177, 774, 833, 913, 1003, 1064, 1215, 1604
BshNI GGYRCC 1 cut(s) 1031
BsiHKAI GWGCWC 1 cut(s) 730
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 1 cut(s) 1211
BsmAI GTCTC 1 cut(s) 1369
BsmBI CGTCTC 1 cut(s) 1369
BsmFI GGGAC 1 cut(s) 446
BsmI GAATGC 1 cut(s) 555
BsnI GGCC 9 cut(s) 7, 177, 774, 833, 913, 1003, 1064, 1215, 1604
Bsp1286I GDGCHC 1 cut(s) 730
Bsp143I GATC 6 cut(s) 361, 602, 1016, 1080, 1113, 1126
BspANI GGCC 9 cut(s) 7, 177, 774, 833, 913, 1003, 1064, 1215, 1604
BspCNI CTCAG 2 cut(s) 1024, 1555
BspLI GGNNCC 4 cut(s) 118, 307, 1033, 1361
BspPI GGATC 4 cut(s) 369, 610, 1024, 1121
BspT107I GGYRCC 1 cut(s) 1031
BsrDI GCAATG 2 cut(s) 530, 914
BsrI ACTGG 4 cut(s) 874, 950, 1130, 1160
BssECI CCNNGG 2 cut(s) 301, 1168
BssMI GATC 6 cut(s) 361, 602, 1016, 1080, 1113, 1126
Bst2UI CCWGG 6 cut(s) 174, 771, 964, 1000, 1170, 1212
Bst4CI ACNGT 4 cut(s) 106, 164, 493, 1414
Bst6I CTCTTC 3 cut(s) 477, 567, 841
BstC8I GCNNGC 4 cut(s) 960, 1091, 1149, 1222
BstDEI CTNAG 4 cut(s) 1011, 1077, 1225, 1563
BstENI CCTNNNNNAGG 1 cut(s) 1209
BstF5I GGATG 4 cut(s) 241, 766, 1164, 1572
BstKTI GATC 6 cut(s) 364, 605, 1019, 1083, 1116, 1129
BstMAI GTCTC 1 cut(s) 1369
BstMBI GATC 6 cut(s) 361, 602, 1016, 1080, 1113, 1126
BstMWI GCNNNNNNNGC 5 cut(s) 995, 1070, 1095, 1221, 1496
BstNI CCWGG 6 cut(s) 174, 771, 964, 1000, 1170, 1212
BstSCI CCNGG 6 cut(s) 172, 769, 962, 998, 1168, 1210
BstSFI CTRYAG 1 cut(s) 780
BstV1I GCAGC 4 cut(s) 547, 917, 1007, 1060
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 2 cut(s) 602, 1113
BstYI RGATCY 2 cut(s) 602, 1113
Bsu36I CCTNAGG 1 cut(s) 1011
BsuI GTATCC 1 cut(s) 1434
BsuRI GGCC 9 cut(s) 7, 177, 774, 833, 913, 1003, 1064, 1215, 1604
BtsCI GGATG 4 cut(s) 241, 766, 1164, 1572
BtsIMutI CAGTG 6 cut(s) 477, 867, 943, 1137, 1560, 1609
Cac8I GCNNGC 4 cut(s) 960, 1091, 1149, 1222
CaiI CAGNNNCTG 2 cut(s) 1217, 1397
Cfr13I GGNCC 6 cut(s) 176, 911, 926, 1214, 1359, 1603
CseI GACGC 1 cut(s) 1386
Csp6I GTAC 5 cut(s) 511, 748, 1032, 1400, 1507
CviAII CATG 4 cut(s) 99, 255, 599, 803
CviQI GTAC 5 cut(s) 511, 748, 1032, 1400, 1507
DdeI CTNAG 4 cut(s) 1011, 1077, 1225, 1563
DpnI GATC 6 cut(s) 363, 604, 1018, 1082, 1115, 1128
DpnII GATC 6 cut(s) 361, 602, 1016, 1080, 1113, 1126
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 1 cut(s) 1062
Eam1104I CTCTTC 3 cut(s) 477, 567, 841
EarI CTCTTC 3 cut(s) 477, 567, 841
Ecl136II GAGCTC 1 cut(s) 728
Eco147I AGGCCT 1 cut(s) 1003
Eco24I GRGCYC 1 cut(s) 730
Eco32I GATATC 1 cut(s) 651
Eco47I GGWCC 2 cut(s) 926, 1359
Eco53kI GAGCTC 1 cut(s) 728
Eco57I CTGAAG 2 cut(s) 501, 667
Eco81I CCTNAGG 1 cut(s) 1011
EcoICRI GAGCTC 1 cut(s) 728
EcoNI CCTNNNNNAGG 1 cut(s) 1209
EcoO109I RGGNCCY 1 cut(s) 1214
EcoRI GAATTC 1 cut(s) 873
EcoRII CCWGG 6 cut(s) 172, 769, 962, 998, 1168, 1210
EcoRV GATATC 1 cut(s) 651
EcoT22I ATGCAT 1 cut(s) 1588
EcoT38I GRGCYC 1 cut(s) 730
Esp3I CGTCTC 1 cut(s) 1369
FaeI CATG 4 cut(s) 102, 258, 602, 806
FaqI GGGAC 1 cut(s) 446
FatI CATG 4 cut(s) 98, 254, 598, 802
Fnu4HI GCNGC 4 cut(s) 536, 906, 996, 1074
FokI GGATG 4 cut(s) 248, 753, 1151, 1559
FriOI GRGCYC 1 cut(s) 730
Fsp4HI GCNGC 4 cut(s) 536, 906, 996, 1074
FspBI CTAG 1 cut(s) 894
GluI GCNGC 4 cut(s) 536, 906, 996, 1074
GsuI CTGGAG 3 cut(s) 358, 1191, 1194
HaeIII GGCC 9 cut(s) 7, 177, 774, 833, 913, 1003, 1064, 1215, 1604
HgaI GACGC 1 cut(s) 1386
Hin1II CATG 4 cut(s) 102, 258, 602, 806
HindIII AAGCTT 3 cut(s) 1091, 1222, 1552
HinfI GANTC 6 cut(s) 297, 319, 548, 688, 752, 843
Hpy166II GTNNAC 3 cut(s) 489, 748, 1108
Hpy188I TCNGA 4 cut(s) 305, 366, 974, 1532
Hpy188III TCNNGA 6 cut(s) 646, 685, 840, 1279, 1313, 1350
Hpy8I GTNNAC 3 cut(s) 489, 748, 1108
Hpy99I CGWCG 1 cut(s) 1268
HpyAV CCTTC 4 cut(s) 399, 622, 716, 1497
HpyCH4III ACNGT 4 cut(s) 106, 164, 493, 1414
HpyCH4IV ACGT 1 cut(s) 762
HpyF10VI GCNNNNNNNGC 5 cut(s) 995, 1070, 1095, 1221, 1496
HpyF3I CTNAG 4 cut(s) 1011, 1077, 1225, 1563
HpySE526I ACGT 1 cut(s) 762
Hsp92II CATG 4 cut(s) 102, 258, 602, 806
KpnI GGTACC 1 cut(s) 1035
Kzo9I GATC 6 cut(s) 361, 602, 1016, 1080, 1113, 1126
LmnI GCTCC 1 cut(s) 725
Lsp1109I GCAGC 4 cut(s) 547, 917, 1007, 1060
LweI GCATC 5 cut(s) 72, 565, 906, 1318, 1384
MaeI CTAG 1 cut(s) 894
MaeII ACGT 1 cut(s) 762
MaeIII GTNAC 5 cut(s) 106, 193, 380, 793, 1132
MalI GATC 6 cut(s) 363, 604, 1018, 1082, 1115, 1128
MboI GATC 6 cut(s) 361, 602, 1016, 1080, 1113, 1126
MboII GAAGA 6 cut(s) 89, 494, 554, 828, 869, 1549
MfeI CAATTG 3 cut(s) 183, 310, 1520
MflI RGATCY 2 cut(s) 602, 1113
MhlI GDGCHC 1 cut(s) 730
MlsI TGGCCA 1 cut(s) 1064
MluNI TGGCCA 1 cut(s) 1064
MlyI GAGTC 1 cut(s) 306
MmeI TCCRAC 4 cut(s) 213, 953, 1318, 1415
Mox20I TGGCCA 1 cut(s) 1064
Mph1103I ATGCAT 1 cut(s) 1588
MroXI GAANNNNTTC 2 cut(s) 121, 1553
MscI TGGCCA 1 cut(s) 1064
MseI TTAA 5 cut(s) 159, 225, 267, 1161, 1184
MslI CAYNNNNRTG 1 cut(s) 1314
Msp20I TGGCCA 1 cut(s) 1064
MspR9I CCNGG 6 cut(s) 174, 771, 964, 1000, 1170, 1212
MunI CAATTG 3 cut(s) 183, 310, 1520
Mva1269I GAATGC 1 cut(s) 555
MvaI CCWGG 6 cut(s) 174, 771, 964, 1000, 1170, 1212
MwoI GCNNNNNNNGC 5 cut(s) 995, 1070, 1095, 1221, 1496
NdeII GATC 6 cut(s) 361, 602, 1016, 1080, 1113, 1126
NlaIII CATG 4 cut(s) 102, 258, 602, 806
NlaIV GGNNCC 4 cut(s) 118, 307, 1033, 1361
NmuCI GTSAC 2 cut(s) 193, 1132
NsiI ATGCAT 1 cut(s) 1588
PceI AGGCCT 1 cut(s) 1003
PcsI WCGNNNNNNNCGW 2 cut(s) 1263, 1269
PctI GAATGC 1 cut(s) 555
PdmI GAANNNNTTC 2 cut(s) 121, 1553
PfeI GAWTC 5 cut(s) 319, 548, 688, 752, 843
PflFI GACNNNGTC 1 cut(s) 1262
PkrI GCNGC 4 cut(s) 537, 907, 997, 1075
PleI GAGTC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 305
PsiI TTATAA 1 cut(s) 587
Psp124BI GAGCTC 1 cut(s) 730
Psp6I CCWGG 6 cut(s) 172, 769, 962, 998, 1168, 1210
PspGI CCWGG 6 cut(s) 172, 769, 962, 998, 1168, 1210
PspN4I GGNNCC 4 cut(s) 118, 307, 1033, 1361
PspPI GGNCC 6 cut(s) 176, 911, 926, 1214, 1359, 1603
PsrI GAACNNNNNNTAC 2 cut(s) 1281, 1313
PstNI CAGNNNCTG 2 cut(s) 1217, 1397
PsuI RGATCY 2 cut(s) 602, 1113
PsyI GACNNNGTC 1 cut(s) 1262
RsaI GTAC 5 cut(s) 512, 749, 1033, 1401, 1508
RsaNI GTAC 5 cut(s) 511, 748, 1032, 1400, 1507
RseI CAYNNNNRTG 1 cut(s) 1314
SacI GAGCTC 1 cut(s) 730
SaqAI TTAA 5 cut(s) 159, 225, 267, 1161, 1184
SatI GCNGC 4 cut(s) 536, 906, 996, 1074
Sau3AI GATC 6 cut(s) 361, 602, 1016, 1080, 1113, 1126
Sau96I GGNCC 6 cut(s) 176, 911, 926, 1214, 1359, 1603
SchI GAGTC 1 cut(s) 306
ScrFI CCNGG 6 cut(s) 174, 771, 964, 1000, 1170, 1212
SduI GDGCHC 1 cut(s) 730
SfaNI GCATC 5 cut(s) 72, 565, 906, 1318, 1384
SfcI CTRYAG 1 cut(s) 780
SinI GGWCC 2 cut(s) 926, 1359
SmiMI CAYNNNNRTG 1 cut(s) 1314
SmlI CTYRAG 2 cut(s) 49, 1480
SmoI CTYRAG 2 cut(s) 49, 1480
SseBI AGGCCT 1 cut(s) 1003
SspMI CTAG 1 cut(s) 894
SstI GAGCTC 1 cut(s) 730
StuI AGGCCT 1 cut(s) 1003
StyD4I CCNGG 6 cut(s) 172, 769, 962, 998, 1168, 1210
TaaI ACNGT 4 cut(s) 106, 164, 493, 1414
TaiI ACGT 1 cut(s) 765
TaqI TCGA 2 cut(s) 880, 1266
TaqII GACCGA 1 cut(s) 1225
TfiI GAWTC 5 cut(s) 319, 548, 688, 752, 843
Tru1I TTAA 5 cut(s) 159, 225, 267, 1161, 1184
Tru9I TTAA 5 cut(s) 159, 225, 267, 1161, 1184
TscAI CASTG 5 cut(s) 484, 874, 950, 1137, 1567
TseFI GTSAC 2 cut(s) 193, 1132
TseI GCWGC 4 cut(s) 535, 905, 995, 1073
Tsp45I GTSAC 2 cut(s) 193, 1132
TspDTI ATGAA 5 cut(s) 252, 587, 755, 814, 968
TspGWI ACGGA 1 cut(s) 129
TspRI CASTG 5 cut(s) 484, 874, 950, 1137, 1567
Tth111I GACNNNGTC 1 cut(s) 1262
VpaK11BI GGWCC 2 cut(s) 926, 1359
XagI CCTNNNNNAGG 1 cut(s) 1209
XapI RAATTY 4 cut(s) 398, 641, 873, 979
XcmI CCANNNNNNNNNTGG 2 cut(s) 127, 333
XmnI GAANNNNTTC 2 cut(s) 121, 1553
XspI CTAG 1 cut(s) 894
Zsp2I ATGCAT 1 cut(s) 1588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.