Rmu_sc0000193.1_g000006

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000193.1
OG0010983
Physical Location & Seq
Reverse (-)
24277 .. 27124
2848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000193.1_g000006.1.cds

Sequence Viewer

Length: 1668 bp
atgaagtgcgccgcaaatgagcttcgattcgattcttcctcggtcctcgcgtccacgctggccctggctgcacccgcgaccatttatataacctcacctggtgttcgatcatttctcacgctcaactacaaagacccttatagatattttacatggacagttacatacggatcagtttctccattaagtgttcctcgacaggctatccttattaatggtcaatttcctggtccaacaattgaggctgtcactaatgacaacataatcattgatgtcattaataagttggatgaacctttcctcattacatggaatggaattaaacagaggaagtccacatgggaagatggagttctgggaaccaattgcccgattcctccaaacacaagctggagatacaagtttcaaacaaaggatcagattggaaccttcagttactttccttcaacgaaacttcatagagctgctggtggttttgggggtttcaatgtggcacacaggtctgtgatagctgtcccatatcccatacccgctgaagagtttaccgtgcttgttggtgattggtacaacactaaccacaaggtattgcaacaaaaattggactctggcaatcctcttcctcttcctgatggtcttcttataaatgggcttcataggtctgcaatcttcactggtcaagaaggaaagacctacaaattcaggatatcaaatgtgggaatagaaacttcaatcaactttaggattcagggtcatacaatgacgcttgttgaagttgaaggagctaataccttgcaagaggtgtatgattcacttgacgttcatccaggccaatctgtagctgttttggtcaccttacacggttcagtcaaggactatttcattgtggcctctacccgattcacaaagcccattctcacaaccactggaattcttcgatatgctggttctagcatcaaggctgcagggccattgcctattggtccaacctatcacattcactggtctatgaagcaagccaggacaatcagattgaatttgacagcaaatgcagccaggcctaaccctcagggctcattccattatggtaccattaaagtagtgaggacacttgttttagccaattctgctactaagatcggtggcaagcttcgctatgctgtaaacaagatatcctatgttgatccagagacccccttgaagctggctgactggtttaacatccctggagtatttaacttcaacacaatcaaggacacacctactccaggccctgcaagttttggtgcctcggtcataggaaccacactccatgacttcgtcgaaatcatattccagaacaccgaacccacacttcaatcttggcatcttgatggatatagcttctacattgttggatatggttctaaaaattggaccccagatatgagcagacgctacaatttggcagacgctgtaccaagacacacagttcaggtatatccatttggttggagtgctgtgatggtgtctttggacaacaagggtatgtggaacttgaggtctgcaatctggtcaagaaggtacttgggtcagcaattgtatatcagagtttggaacgatgagaaaagcttcttcactgagaatgatatccctcaaaatgcattattttgtggcaaggcgcatcactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

555

Amino Acids

61.82

Weight (kDa)

9.34

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010983)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23630 AT2G23630 AT4G37160 AT4G37160
fragaria_vesca FvH4_1g14980 FvH4_1g14980
malus_domestica MD02G1163600.v1.1
prunus_persica Prupe.7G140600_v2.0.a1 Prupe.7G140600_v2.0.a1
pyrus_communis pycom02g13000
rosa_chinensis RchiOBHm_Chr2g0104041
rosa_laevigata RLG00000017280
rosa_multiflora Rmu_sc0000193.1_g000006
rosa_roxburghii Rroxscaffold_2G00139180
rosa_rugosa Rorug02G0119000
rosa_samantha Rh2AG168100 Rh2BG175100 Rh2CG174000 Rh2DG173600
rosa_wichuraiana Rw2G013150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 641
Acc65I GGTACC 1 cut(s) 1085
AccB1I GGYRCC 2 cut(s) 1085, 1283
AccII CGCG 2 cut(s) 50, 77
AciI CCGC 3 cut(s) 12, 75, 531
AclWI GGATC 3 cut(s) 178, 423, 1175
AcsI RAATTY 3 cut(s) 695, 927, 1033
AcuI CTGAAG 2 cut(s) 415, 555
AdeI CACNNNGTG 1 cut(s) 101
AfaI GTAC 4 cut(s) 566, 1087, 1455, 1562
AfiI CCNNNNNNNGG 1 cut(s) 1265
AjnI CCWGG 8 cut(s) 63, 97, 226, 823, 1016, 1052, 1222, 1264
Alw26I GTCTC 1 cut(s) 1181
AlwI GGATC 3 cut(s) 178, 423, 1175
AlwNI CAGNNNCTG 2 cut(s) 1271, 1451
AoxI GGCC 6 cut(s) 60, 826, 885, 965, 1055, 1267
ApeKI GCWGC 4 cut(s) 68, 464, 959, 1049
ApoI RAATTY 3 cut(s) 695, 927, 1033
AseI ATTAAT 2 cut(s) 213, 279
Asp700I GAANNNNTTC 1 cut(s) 1607
Asp718I GGTACC 1 cut(s) 1085
AspLEI GCGC 2 cut(s) 11, 1660
AspS9I GGNCC 7 cut(s) 43, 61, 230, 965, 980, 1268, 1413
AsuHPI GGTGA 3 cut(s) 87, 569, 841
AvaII GGWCC 4 cut(s) 43, 230, 980, 1413
AxyI CCTNAGG 1 cut(s) 1065
BaeI ACNNNNGTAYC 2 cut(s) 1552, 1585
BanI GGYRCC 2 cut(s) 1085, 1283
BanII GRGCYC 1 cut(s) 1073
BbsI GAAGAC 1 cut(s) 626
BbvI GCAGC 4 cut(s) 55, 451, 946, 1061
BccI CCATC 4 cut(s) 341, 623, 1364, 1495
BciT130I CCWGG 8 cut(s) 65, 99, 228, 825, 1018, 1054, 1224, 1266
BcoDI GTCTC 1 cut(s) 1181
BfaI CTAG 1 cut(s) 948
BfmI CTRYAG 2 cut(s) 834, 960
BisI GCNGC 5 cut(s) 12, 69, 465, 960, 1050
BlsI GCNGC 5 cut(s) 13, 70, 466, 961, 1051
Bme1390I CCNGG 8 cut(s) 65, 99, 228, 825, 1018, 1054, 1224, 1266
Bme18I GGWCC 4 cut(s) 43, 230, 980, 1413
BmgT120I GGNCC 7 cut(s) 43, 61, 230, 965, 980, 1268, 1413
BmiI GGNNCC 6 cut(s) 361, 427, 1087, 1285, 1300, 1415
BmrFI CCNGG 8 cut(s) 65, 99, 228, 825, 1018, 1054, 1224, 1266
BmsI GCATC 2 cut(s) 960, 1372
BpiI GAAGAC 1 cut(s) 626
BpmI CTGGAG 3 cut(s) 412, 1245, 1248
BpuEI CTTGAG 1 cut(s) 1555
BsaI GGTCTC 1 cut(s) 1181
BsaJI CCNNGG 4 cut(s) 39, 63, 1222, 1287
BsaXI ACNNNNNCTCC 2 cut(s) 1246, 1276
Bsc4I CCNNNNNNNGG 1 cut(s) 1265
Bse1I ACTGG 4 cut(s) 676, 928, 1004, 1214
Bse21I CCTNAGG 1 cut(s) 1065
Bse3DI GCAATG 1 cut(s) 968
BseBI CCWGG 8 cut(s) 65, 99, 228, 825, 1018, 1054, 1224, 1266
BseDI CCNNGG 4 cut(s) 39, 63, 1222, 1287
BseGI GGATG 3 cut(s) 295, 820, 1218
BseLI CCNNNNNNNGG 1 cut(s) 1265
BseMI GCAATG 1 cut(s) 968
BseMII CTCAG 2 cut(s) 1079, 1608
BseNI ACTGG 4 cut(s) 676, 928, 1004, 1214
BseXI GCAGC 4 cut(s) 55, 451, 946, 1061
BsgI GTGCAG 1 cut(s) 54
Bsh1236I CGCG 2 cut(s) 50, 77
BshFI GGCC 6 cut(s) 62, 828, 887, 967, 1057, 1269
BshNI GGYRCC 2 cut(s) 1085, 1283
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 1 cut(s) 1265
BsmAI GTCTC 1 cut(s) 1181
BsmFI GGGAC 1 cut(s) 500
BsnI GGCC 6 cut(s) 62, 828, 887, 967, 1057, 1269
Bso31I GGTCTC 1 cut(s) 1181
Bsp1286I GDGCHC 1 cut(s) 1073
Bsp143I GATC 5 cut(s) 107, 170, 415, 1134, 1180
BspACI CCGC 3 cut(s) 12, 75, 531
BspANI GGCC 6 cut(s) 62, 828, 887, 967, 1057, 1269
BspCNI CTCAG 2 cut(s) 1078, 1609
BspFNI CGCG 2 cut(s) 50, 77
BspLI GGNNCC 6 cut(s) 361, 427, 1087, 1285, 1300, 1415
BspMAI CTGCAG 1 cut(s) 964
BspPI GGATC 3 cut(s) 178, 423, 1175
BspT107I GGYRCC 2 cut(s) 1085, 1283
BspTNI GGTCTC 1 cut(s) 1181
BsrDI GCAATG 1 cut(s) 968
BsrI ACTGG 4 cut(s) 676, 928, 1004, 1214
BssECI CCNNGG 4 cut(s) 39, 63, 1222, 1287
BssMI GATC 5 cut(s) 107, 170, 415, 1134, 1180
Bst2UI CCWGG 8 cut(s) 65, 99, 228, 825, 1018, 1054, 1224, 1266
Bst4CI ACNGT 4 cut(s) 160, 547, 860, 1468
Bst6I CTCTTC 3 cut(s) 531, 621, 627
BstC8I GCNNGC 4 cut(s) 60, 1014, 1145, 1203
BstDEI CTNAG 3 cut(s) 1065, 1131, 1617
BstEII GGTNACC 1 cut(s) 847
BstENI CCTNNNNNAGG 1 cut(s) 1263
BstF5I GGATG 3 cut(s) 295, 820, 1218
BstFNI CGCG 2 cut(s) 50, 77
BstHHI GCGC 2 cut(s) 11, 1660
BstKTI GATC 5 cut(s) 110, 173, 418, 1137, 1183
BstMAI GTCTC 1 cut(s) 1181
BstMBI GATC 5 cut(s) 107, 170, 415, 1134, 1180
BstMWI GCNNNNNNNGC 5 cut(s) 68, 74, 1049, 1124, 1149
BstNI CCWGG 8 cut(s) 65, 99, 228, 825, 1018, 1054, 1224, 1266
BstPI GGTNACC 1 cut(s) 847
BstSCI CCNGG 8 cut(s) 63, 97, 226, 823, 1016, 1052, 1222, 1264
BstSFI CTRYAG 2 cut(s) 834, 960
BstUI CGCG 2 cut(s) 50, 77
BstV1I GCAGC 4 cut(s) 55, 451, 946, 1061
BstV2I GAAGAC 1 cut(s) 626
BstXI CCANNNNNNTGG 1 cut(s) 1488
Bsu36I CCTNAGG 1 cut(s) 1065
BsuRI GGCC 6 cut(s) 62, 828, 887, 967, 1057, 1269
BtsCI GGATG 3 cut(s) 295, 820, 1218
BtsIMutI CAGTG 5 cut(s) 669, 921, 997, 1614, 1663
Cac8I GCNNGC 4 cut(s) 60, 1014, 1145, 1203
CaiI CAGNNNCTG 2 cut(s) 1271, 1451
CfoI GCGC 2 cut(s) 11, 1660
Cfr13I GGNCC 7 cut(s) 43, 61, 230, 965, 980, 1268, 1413
CseI GACGC 4 cut(s) 39, 769, 1440, 1457
CsiI ACCWGGT 1 cut(s) 97
Csp6I GTAC 4 cut(s) 565, 1086, 1454, 1561
CviAII CATG 4 cut(s) 153, 309, 339, 1310
CviQI GTAC 4 cut(s) 565, 1086, 1454, 1561
DdeI CTNAG 3 cut(s) 1065, 1131, 1617
DpnI GATC 5 cut(s) 109, 172, 417, 1136, 1182
DpnII GATC 5 cut(s) 107, 170, 415, 1134, 1180
DraIII CACNNNGTG 1 cut(s) 101
Eam1104I CTCTTC 3 cut(s) 531, 621, 627
EarI CTCTTC 3 cut(s) 531, 621, 627
Eco147I AGGCCT 1 cut(s) 1057
Eco24I GRGCYC 1 cut(s) 1073
Eco31I GGTCTC 1 cut(s) 1181
Eco32I GATATC 3 cut(s) 705, 1170, 1627
Eco47I GGWCC 4 cut(s) 43, 230, 980, 1413
Eco57I CTGAAG 2 cut(s) 415, 555
Eco81I CCTNAGG 1 cut(s) 1065
Eco91I GGTNACC 1 cut(s) 847
EcoNI CCTNNNNNAGG 1 cut(s) 1263
EcoO109I RGGNCCY 1 cut(s) 1268
EcoO65I GGTNACC 1 cut(s) 847
EcoRI GAATTC 1 cut(s) 927
EcoRII CCWGG 8 cut(s) 63, 97, 226, 823, 1016, 1052, 1222, 1264
EcoRV GATATC 3 cut(s) 705, 1170, 1627
EcoT22I ATGCAT 1 cut(s) 1642
EcoT38I GRGCYC 1 cut(s) 1073
FaeI CATG 4 cut(s) 156, 312, 342, 1313
FaqI GGGAC 1 cut(s) 500
FatI CATG 4 cut(s) 152, 308, 338, 1309
FauI CCCGC 2 cut(s) 82, 538
Fnu4HI GCNGC 5 cut(s) 12, 69, 465, 960, 1050
FokI GGATG 3 cut(s) 302, 807, 1205
FriOI GRGCYC 1 cut(s) 1073
Fsp4HI GCNGC 5 cut(s) 12, 69, 465, 960, 1050
FspBI CTAG 1 cut(s) 948
GlaI GCGC 2 cut(s) 10, 1659
GluI GCNGC 5 cut(s) 12, 69, 465, 960, 1050
GsuI CTGGAG 3 cut(s) 412, 1245, 1248
HaeIII GGCC 6 cut(s) 62, 828, 887, 967, 1057, 1269
HgaI GACGC 4 cut(s) 39, 769, 1440, 1457
HhaI GCGC 2 cut(s) 11, 1660
Hin1II CATG 4 cut(s) 156, 312, 342, 1313
Hin6I GCGC 2 cut(s) 9, 1658
HinP1I GCGC 2 cut(s) 9, 1658
HindIII AAGCTT 2 cut(s) 1145, 1606
HinfI GANTC 7 cut(s) 27, 32, 373, 602, 742, 806, 897
HphI GGTGA 3 cut(s) 87, 569, 841
Hpy166II GTNNAC 4 cut(s) 54, 336, 543, 1162
Hpy188I TCNGA 3 cut(s) 420, 1028, 1586
Hpy188III TCNNGA 7 cut(s) 626, 677, 700, 1184, 1333, 1367, 1554
Hpy8I GTNNAC 4 cut(s) 54, 336, 543, 1162
Hpy99I CGWCG 1 cut(s) 1322
HpyAV CCTTC 5 cut(s) 439, 453, 674, 770, 1551
HpyCH4III ACNGT 4 cut(s) 160, 547, 860, 1468
HpyCH4IV ACGT 1 cut(s) 816
HpyCH4V TGCA 9 cut(s) 71, 589, 662, 793, 962, 1049, 1274, 1544, 1640
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 74, 1049, 1124, 1149
HpyF3I CTNAG 3 cut(s) 1065, 1131, 1617
HpySE526I ACGT 1 cut(s) 816
Hsp92II CATG 4 cut(s) 156, 312, 342, 1313
HspAI GCGC 2 cut(s) 9, 1658
KpnI GGTACC 1 cut(s) 1089
Kzo9I GATC 5 cut(s) 107, 170, 415, 1134, 1180
LmnI GCTCC 1 cut(s) 779
Lsp1109I GCAGC 4 cut(s) 55, 451, 946, 1061
LweI GCATC 2 cut(s) 960, 1372
MabI ACCWGGT 1 cut(s) 97
MaeI CTAG 1 cut(s) 948
MaeII ACGT 1 cut(s) 816
MaeIII GTNAC 4 cut(s) 160, 247, 434, 847
MalI GATC 5 cut(s) 109, 172, 417, 1136, 1182
MboI GATC 5 cut(s) 107, 170, 415, 1134, 1180
MboII GAAGA 9 cut(s) 27, 356, 548, 608, 614, 626, 658, 923, 1603
MfeI CAATTG 3 cut(s) 237, 364, 1574
MhlI GDGCHC 1 cut(s) 1073
MlyI GAGTC 1 cut(s) 596
MmeI TCCRAC 5 cut(s) 257, 267, 1007, 1372, 1469
Mph1103I ATGCAT 1 cut(s) 1642
MroXI GAANNNNTTC 1 cut(s) 1607
MseI TTAA 7 cut(s) 185, 213, 279, 321, 1092, 1215, 1233
MslI CAYNNNNRTG 1 cut(s) 1368
MspA1I CMGCKG 1 cut(s) 533
MspR9I CCNGG 8 cut(s) 65, 99, 228, 825, 1018, 1054, 1224, 1266
MunI CAATTG 3 cut(s) 237, 364, 1574
MvaI CCWGG 8 cut(s) 65, 99, 228, 825, 1018, 1054, 1224, 1266
MvnI CGCG 2 cut(s) 50, 77
MwoI GCNNNNNNNGC 5 cut(s) 68, 74, 1049, 1124, 1149
NdeII GATC 5 cut(s) 107, 170, 415, 1134, 1180
NlaIII CATG 4 cut(s) 156, 312, 342, 1313
NlaIV GGNNCC 6 cut(s) 361, 427, 1087, 1285, 1300, 1415
NmuCI GTSAC 2 cut(s) 247, 847
NsiI ATGCAT 1 cut(s) 1642
PceI AGGCCT 1 cut(s) 1057
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 1607
PfeI GAWTC 6 cut(s) 27, 32, 373, 742, 806, 897
PflFI GACNNNGTC 1 cut(s) 1316
PkrI GCNGC 5 cut(s) 13, 70, 466, 961, 1051
PleI GAGTC 1 cut(s) 596
PpsI GAGTC 1 cut(s) 596
PshBI ATTAAT 2 cut(s) 213, 279
PsiI TTATAA 1 cut(s) 641
Psp6I CCWGG 8 cut(s) 63, 97, 226, 823, 1016, 1052, 1222, 1264
PspEI GGTNACC 1 cut(s) 847
PspGI CCWGG 8 cut(s) 63, 97, 226, 823, 1016, 1052, 1222, 1264
PspN4I GGNNCC 6 cut(s) 361, 427, 1087, 1285, 1300, 1415
PspPI GGNCC 7 cut(s) 43, 61, 230, 965, 980, 1268, 1413
PstI CTGCAG 1 cut(s) 964
PstNI CAGNNNCTG 2 cut(s) 1271, 1451
PsyI GACNNNGTC 1 cut(s) 1316
RsaI GTAC 4 cut(s) 566, 1087, 1455, 1562
RsaNI GTAC 4 cut(s) 565, 1086, 1454, 1561
RseI CAYNNNNRTG 1 cut(s) 1368
SaqAI TTAA 7 cut(s) 185, 213, 279, 321, 1092, 1215, 1233
SatI GCNGC 5 cut(s) 12, 69, 465, 960, 1050
Sau3AI GATC 5 cut(s) 107, 170, 415, 1134, 1180
Sau96I GGNCC 7 cut(s) 43, 61, 230, 965, 980, 1268, 1413
SchI GAGTC 1 cut(s) 596
ScrFI CCNGG 8 cut(s) 65, 99, 228, 825, 1018, 1054, 1224, 1266
SduI GDGCHC 1 cut(s) 1073
SexAI ACCWGGT 1 cut(s) 97
SfaNI GCATC 2 cut(s) 960, 1372
SfcI CTRYAG 2 cut(s) 834, 960
SinI GGWCC 4 cut(s) 43, 230, 980, 1413
SmiMI CAYNNNNRTG 1 cut(s) 1368
SmlI CTYRAG 1 cut(s) 1534
SmoI CTYRAG 1 cut(s) 1534
SseBI AGGCCT 1 cut(s) 1057
SsiI CCGC 3 cut(s) 12, 75, 531
SspMI CTAG 1 cut(s) 948
StuI AGGCCT 1 cut(s) 1057
StyD4I CCNGG 8 cut(s) 63, 97, 226, 823, 1016, 1052, 1222, 1264
TaaI ACNGT 4 cut(s) 160, 547, 860, 1468
TaiI ACGT 1 cut(s) 819
TaqI TCGA 6 cut(s) 25, 30, 106, 196, 934, 1320
TaqII GACCGA 2 cut(s) 31, 1279
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 6 cut(s) 27, 32, 373, 742, 806, 897
Tru1I TTAA 7 cut(s) 185, 213, 279, 321, 1092, 1215, 1233
Tru9I TTAA 7 cut(s) 185, 213, 279, 321, 1092, 1215, 1233
TscAI CASTG 4 cut(s) 676, 928, 1004, 1621
TseFI GTSAC 2 cut(s) 247, 847
TseI GCWGC 4 cut(s) 68, 464, 959, 1049
Tsp45I GTSAC 2 cut(s) 247, 847
TspDTI ATGAA 7 cut(s) 17, 306, 446, 641, 809, 868, 1022
TspGWI ACGGA 1 cut(s) 183
TspRI CASTG 4 cut(s) 676, 928, 1004, 1621
Tth111I GACNNNGTC 1 cut(s) 1316
VpaK11BI GGWCC 4 cut(s) 43, 230, 980, 1413
VspI ATTAAT 2 cut(s) 213, 279
XagI CCTNNNNNAGG 1 cut(s) 1263
XapI RAATTY 3 cut(s) 695, 927, 1033
XcmI CCANNNNNNNNNTGG 2 cut(s) 61, 387
XmnI GAANNNNTTC 1 cut(s) 1607
XspI CTAG 1 cut(s) 948
Zsp2I ATGCAT 1 cut(s) 1642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.