Rh2AG168100

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010983
Physical Location & Seq
Reverse (-)
15913673 .. 15917105
3433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG168100.1

Sequence Viewer

Length: 1770 bp
ATGAAGTGCGCCGCAAATGAGCTTCGATTCTTCCTCGGTCCTCGAGTCCACGCTGGCCCTGGCTGCACCCGCGACCATTTATATAACCTCACCTGGTGTTCGATCATTTCTCACGCTCAACTACAGAGCTTGGGGGAATATATAGGAGCTTATACAATGAGGCCAACCTCTTTCCTGCTTTTTTTTCTTGGAACATTGGTTGGCTTGAGTGCTTTCTGCAACATCAATGCAGAAGACCCTTATAGATATTTTACATGGACAGTTACATACGGATCAGTTTCTCCATTGGGTGTTCCTCGACAGGCTATCCTTATTAATGGTCAATTTCCTGGTCCAACAATTGAGGCTGTGACTAATGACAACATAATCGTTGATGTCATTAATAAGTTGGATGAACCTTTCCTCATTACATGGAATGGAATTAAACAGAGGAAGTCCACATGGGAAGATGGAGTTCTCGGAACCAATTGCCCGATTCCTCCAAACACAAGCTGGAGATACAAGTTTCAAACAAAGGATCAGATTGGAACCTTCAGTTACTTTCCTTCAACGAAACTTCATAGAGCTGCTGGTGGTTTTGGGGGTTTCAATGTGGCACACAGGTCTGTGATAGCTGTCCCATATCCCATACCCGCTGAAGAGTTTACCGTGCTTGTTGGTGATTGGTACAACACTAACCACAAGGTATTGCAACAAAAATTGGACTCTGGCAATCCTCTTCCTCTTCCTGATGGTCTTCTTATAAATGGGCTTCATAGGTCTGCAATCTTCACTGGTCAAGAAGGAAAGACCTACAAATTCAGGATATCAAATGTGGGAATAGCAACTTCAATCAACTTTAGGATTCAGGGTCATACAATGACGCTTGTTGAAGTTGAAGGAGCTAATACCTTGCAAGAGGTGTATGATTCACTTGACGTTCATCCAGGCCAATCTGTAGCTGTTTTGGTCACCTTACACGGTTCAGTCAAGGACTATTTCATTGTGGCCTCTACCCGATTCACAAAGCCCATTCTCACAACCACTGGAATTCTTCGATATGCTGGTTCTAGCATCAAGGCTGCAGGGCCATTGCCTATTGGTCCAACCTATCACATTCACTGGTCTATGAAGCAAGCCAGGACAATCAGATTGAATTTGACAGCAAATGCAGCCAGGCCTAACCCTCAGGGCTCATTCCATTATGGTACCATTAAAGTAGTGAGAACACTTGTTTTAGCCAATTCTGCTACTAAGATCGGTGGCAAGCTTCGCTATGCTGTAAACAAGATATCCTATGTTGATCCAGAGACCCCCTTGAAGCTGGCTGACTGGTTTAACATCCCTGGAGTATTTAACTTCAACACAATCAAGGACACACCTACTCCAGGCCCTGCAAGTTTTGGTGACTCGGTCATAGGAACCACACTCCATGACTTCGTCGAAATCGTATTCCAGAACACCGAACCCACACTTCAATCTTGGCATCTTGATGGATATAGCTTCTACATTGTTGGATATGGTTCTAAAAATTGGACCCCAGATATGAGGAGACGCTACAATTTGGCAGACGCTGTACCAAGACACACAGTTCAGGTATATCCATTTGGTTGGAGTGCTGTGATGGTGTCTTTGGACAACAAGGGTATGTGGAACTTGAGGTCTGCAATCTGGTCAAGAAGGTACTTGGGTCAGCAATTGTATATCAGAGTTTGGAACGATGAGAAAAGCTTCTTCACTGAGAATGATATCCCTCAAAATGCATTATTTTGTGGCAAGGCCCATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

589

Amino Acids

65.78

Weight (kDa)

9.23

Isoelectric Point (pI)

31.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 88 - 201 1.4e-39 Multicopper oxidase
Cu-oxidase PF00394 213 - 349 1.3e-39 Multicopper oxidase
Cu-oxidase_2 PF07731 431 - 569 2.2e-31 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010983)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23630 AT2G23630 AT4G37160 AT4G37160
fragaria_vesca FvH4_1g14980 FvH4_1g14980
malus_domestica MD02G1163600.v1.1
prunus_persica Prupe.7G140600_v2.0.a1 Prupe.7G140600_v2.0.a1
pyrus_communis pycom02g13000
rosa_chinensis RchiOBHm_Chr2g0104041
rosa_laevigata RLG00000017280
rosa_multiflora Rmu_sc0000193.1_g000006
rosa_roxburghii Rroxscaffold_2G00139180
rosa_rugosa Rorug02G0119000
rosa_samantha Rh2AG168100 Rh2BG175100 Rh2CG174000 Rh2DG173600
rosa_wichuraiana Rw2G013150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 743
Acc65I GGTACC 1 cut(s) 1187
AccB1I GGYRCC 1 cut(s) 1187
AccII CGCG 1 cut(s) 72
AciI CCGC 3 cut(s) 12, 70, 633
AclWI GGATC 3 cut(s) 280, 525, 1277
AcsI RAATTY 3 cut(s) 797, 1029, 1135
AcuI CTGAAG 2 cut(s) 517, 657
AdeI CACNNNGTG 1 cut(s) 96
AfaI GTAC 4 cut(s) 668, 1189, 1557, 1664
AfiI CCNNNNNNNGG 1 cut(s) 1367
AjnI CCWGG 8 cut(s) 58, 92, 328, 925, 1118, 1154, 1324, 1366
Alw26I GTCTC 2 cut(s) 1283, 1525
AlwI GGATC 3 cut(s) 280, 525, 1277
AlwNI CAGNNNCTG 2 cut(s) 1373, 1553
Ama87I CYCGRG 1 cut(s) 42
AoxI GGCC 8 cut(s) 55, 161, 928, 987, 1067, 1157, 1369, 1758
ApeKI GCWGC 4 cut(s) 63, 566, 1061, 1151
ApoI RAATTY 3 cut(s) 797, 1029, 1135
AseI ATTAAT 2 cut(s) 315, 381
Asp700I GAANNNNTTC 1 cut(s) 1709
Asp718I GGTACC 1 cut(s) 1187
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 8 cut(s) 38, 56, 332, 1067, 1082, 1370, 1515, 1759
AsuHPI GGTGA 4 cut(s) 82, 671, 943, 1397
AvaI CYCGRG 1 cut(s) 42
AvaII GGWCC 4 cut(s) 38, 332, 1082, 1515
AxyI CCTNAGG 1 cut(s) 1167
BaeI ACNNNNGTAYC 2 cut(s) 1654, 1687
BanI GGYRCC 1 cut(s) 1187
BanII GRGCYC 1 cut(s) 1175
BbsI GAAGAC 2 cut(s) 240, 728
BbvI GCAGC 4 cut(s) 50, 553, 1048, 1163
BccI CCATC 4 cut(s) 443, 725, 1466, 1597
BciT130I CCWGG 8 cut(s) 60, 94, 330, 927, 1120, 1156, 1326, 1368
BcoDI GTCTC 2 cut(s) 1283, 1525
BfaI CTAG 1 cut(s) 1050
BfmI CTRYAG 3 cut(s) 122, 936, 1062
BisI GCNGC 5 cut(s) 12, 64, 567, 1062, 1152
BlsI GCNGC 5 cut(s) 13, 65, 568, 1063, 1153
Bme1390I CCNGG 8 cut(s) 60, 94, 330, 927, 1120, 1156, 1326, 1368
Bme18I GGWCC 4 cut(s) 38, 332, 1082, 1515
BmeT110I CYCGRG 1 cut(s) 42
BmgT120I GGNCC 8 cut(s) 38, 56, 332, 1067, 1082, 1370, 1515, 1759
BmiI GGNNCC 5 cut(s) 463, 529, 1189, 1402, 1517
BmrFI CCNGG 8 cut(s) 60, 94, 330, 927, 1120, 1156, 1326, 1368
BmsI GCATC 2 cut(s) 1062, 1474
BpiI GAAGAC 2 cut(s) 240, 728
BpmI CTGGAG 3 cut(s) 514, 1347, 1350
BpuEI CTTGAG 2 cut(s) 226, 1657
BsaI GGTCTC 1 cut(s) 1283
BsaJI CCNNGG 3 cut(s) 34, 58, 1324
BsaXI ACNNNNNCTCC 2 cut(s) 1348, 1378
Bsc4I CCNNNNNNNGG 1 cut(s) 1367
Bse1I ACTGG 4 cut(s) 778, 1030, 1106, 1316
Bse21I CCTNAGG 1 cut(s) 1167
Bse3DI GCAATG 1 cut(s) 1070
BseBI CCWGG 8 cut(s) 60, 94, 330, 927, 1120, 1156, 1326, 1368
BseDI CCNNGG 3 cut(s) 34, 58, 1324
BseGI GGATG 3 cut(s) 397, 922, 1320
BseLI CCNNNNNNNGG 1 cut(s) 1367
BseMI GCAATG 1 cut(s) 1070
BseMII CTCAG 2 cut(s) 1181, 1710
BseNI ACTGG 4 cut(s) 778, 1030, 1106, 1316
BseRI GAGGAG 1 cut(s) 1543
BseXI GCAGC 4 cut(s) 50, 553, 1048, 1163
BsgI GTGCAG 1 cut(s) 49
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 8 cut(s) 57, 163, 930, 989, 1069, 1159, 1371, 1760
BshNI GGYRCC 1 cut(s) 1187
BsiHKCI CYCGRG 1 cut(s) 42
BslFI GGGAC 1 cut(s) 602
BslI CCNNNNNNNGG 1 cut(s) 1367
BsmAI GTCTC 2 cut(s) 1283, 1525
BsmBI CGTCTC 1 cut(s) 1525
BsmFI GGGAC 1 cut(s) 602
BsnI GGCC 8 cut(s) 57, 163, 930, 989, 1069, 1159, 1371, 1760
Bso31I GGTCTC 1 cut(s) 1283
BsoBI CYCGRG 1 cut(s) 42
Bsp1286I GDGCHC 1 cut(s) 1175
Bsp143I GATC 5 cut(s) 102, 272, 517, 1236, 1282
BspACI CCGC 3 cut(s) 12, 70, 633
BspANI GGCC 8 cut(s) 57, 163, 930, 989, 1069, 1159, 1371, 1760
BspCNI CTCAG 2 cut(s) 1180, 1711
BspFNI CGCG 1 cut(s) 72
BspLI GGNNCC 5 cut(s) 463, 529, 1189, 1402, 1517
BspMAI CTGCAG 1 cut(s) 1066
BspPI GGATC 3 cut(s) 280, 525, 1277
BspT107I GGYRCC 1 cut(s) 1187
BspTNI GGTCTC 1 cut(s) 1283
BsrDI GCAATG 1 cut(s) 1070
BsrI ACTGG 4 cut(s) 778, 1030, 1106, 1316
BssECI CCNNGG 3 cut(s) 34, 58, 1324
BssMI GATC 5 cut(s) 102, 272, 517, 1236, 1282
Bst2UI CCWGG 8 cut(s) 60, 94, 330, 927, 1120, 1156, 1326, 1368
Bst4CI ACNGT 4 cut(s) 262, 649, 962, 1570
Bst6I CTCTTC 3 cut(s) 633, 723, 729
BstC8I GCNNGC 4 cut(s) 55, 1116, 1247, 1305
BstDEI CTNAG 3 cut(s) 1167, 1233, 1719
BstEII GGTNACC 1 cut(s) 949
BstENI CCTNNNNNAGG 1 cut(s) 1365
BstF5I GGATG 3 cut(s) 397, 922, 1320
BstFNI CGCG 1 cut(s) 72
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 5 cut(s) 105, 275, 520, 1239, 1285
BstMAI GTCTC 2 cut(s) 1283, 1525
BstMBI GATC 5 cut(s) 102, 272, 517, 1236, 1282
BstMWI GCNNNNNNNGC 5 cut(s) 63, 69, 1151, 1226, 1251
BstNI CCWGG 8 cut(s) 60, 94, 330, 927, 1120, 1156, 1326, 1368
BstPI GGTNACC 1 cut(s) 949
BstSCI CCNGG 8 cut(s) 58, 92, 328, 925, 1118, 1154, 1324, 1366
BstSFI CTRYAG 3 cut(s) 122, 936, 1062
BstUI CGCG 1 cut(s) 72
BstV1I GCAGC 4 cut(s) 50, 553, 1048, 1163
BstV2I GAAGAC 2 cut(s) 240, 728
BstXI CCANNNNNNTGG 1 cut(s) 1590
Bsu36I CCTNAGG 1 cut(s) 1167
BsuRI GGCC 8 cut(s) 57, 163, 930, 989, 1069, 1159, 1371, 1760
BtsCI GGATG 3 cut(s) 397, 922, 1320
BtsIMutI CAGTG 5 cut(s) 771, 1023, 1099, 1716, 1765
Cac8I GCNNGC 4 cut(s) 55, 1116, 1247, 1305
CaiI CAGNNNCTG 2 cut(s) 1373, 1553
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 8 cut(s) 38, 56, 332, 1067, 1082, 1370, 1515, 1759
CseI GACGC 3 cut(s) 871, 1542, 1559
CsiI ACCWGGT 1 cut(s) 92
Csp6I GTAC 4 cut(s) 667, 1188, 1556, 1663
CviAII CATG 4 cut(s) 255, 411, 441, 1412
CviQI GTAC 4 cut(s) 667, 1188, 1556, 1663
DdeI CTNAG 3 cut(s) 1167, 1233, 1719
DpnI GATC 5 cut(s) 104, 274, 519, 1238, 1284
DpnII GATC 5 cut(s) 102, 272, 517, 1236, 1282
DraIII CACNNNGTG 1 cut(s) 96
Eam1104I CTCTTC 3 cut(s) 633, 723, 729
EarI CTCTTC 3 cut(s) 633, 723, 729
Eco147I AGGCCT 1 cut(s) 1159
Eco24I GRGCYC 1 cut(s) 1175
Eco31I GGTCTC 1 cut(s) 1283
Eco32I GATATC 3 cut(s) 807, 1272, 1729
Eco47I GGWCC 4 cut(s) 38, 332, 1082, 1515
Eco57I CTGAAG 2 cut(s) 517, 657
Eco81I CCTNAGG 1 cut(s) 1167
Eco88I CYCGRG 1 cut(s) 42
Eco91I GGTNACC 1 cut(s) 949
EcoNI CCTNNNNNAGG 1 cut(s) 1365
EcoO109I RGGNCCY 1 cut(s) 1370
EcoO65I GGTNACC 1 cut(s) 949
EcoRI GAATTC 1 cut(s) 1029
EcoRII CCWGG 8 cut(s) 58, 92, 328, 925, 1118, 1154, 1324, 1366
EcoRV GATATC 3 cut(s) 807, 1272, 1729
EcoT22I ATGCAT 1 cut(s) 1744
EcoT38I GRGCYC 1 cut(s) 1175
Esp3I CGTCTC 1 cut(s) 1525
FaeI CATG 4 cut(s) 258, 414, 444, 1415
FaqI GGGAC 1 cut(s) 602
FatI CATG 4 cut(s) 254, 410, 440, 1411
FauI CCCGC 2 cut(s) 77, 640
Fnu4HI GCNGC 5 cut(s) 12, 64, 567, 1062, 1152
FokI GGATG 3 cut(s) 404, 909, 1307
FriOI GRGCYC 1 cut(s) 1175
Fsp4HI GCNGC 5 cut(s) 12, 64, 567, 1062, 1152
FspBI CTAG 1 cut(s) 1050
GlaI GCGC 1 cut(s) 10
GluI GCNGC 5 cut(s) 12, 64, 567, 1062, 1152
GsuI CTGGAG 3 cut(s) 514, 1347, 1350
HaeIII GGCC 8 cut(s) 57, 163, 930, 989, 1069, 1159, 1371, 1760
HgaI GACGC 3 cut(s) 871, 1542, 1559
HhaI GCGC 1 cut(s) 11
Hin1II CATG 4 cut(s) 258, 414, 444, 1415
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HindIII AAGCTT 2 cut(s) 1247, 1708
HinfI GANTC 8 cut(s) 27, 45, 475, 704, 844, 908, 999, 1388
HphI GGTGA 4 cut(s) 82, 671, 943, 1397
Hpy166II GTNNAC 4 cut(s) 49, 438, 645, 1264
Hpy188I TCNGA 4 cut(s) 461, 522, 1130, 1688
Hpy188III TCNNGA 7 cut(s) 728, 779, 802, 1286, 1435, 1469, 1656
Hpy8I GTNNAC 4 cut(s) 49, 438, 645, 1264
Hpy99I CGWCG 1 cut(s) 1424
HpyAV CCTTC 5 cut(s) 541, 555, 776, 872, 1653
HpyCH4III ACNGT 4 cut(s) 262, 649, 962, 1570
HpyCH4IV ACGT 1 cut(s) 918
HpyF10VI GCNNNNNNNGC 5 cut(s) 63, 69, 1151, 1226, 1251
HpyF3I CTNAG 3 cut(s) 1167, 1233, 1719
HpySE526I ACGT 1 cut(s) 918
Hsp92II CATG 4 cut(s) 258, 414, 444, 1415
HspAI GCGC 1 cut(s) 9
KpnI GGTACC 1 cut(s) 1191
Kzo9I GATC 5 cut(s) 102, 272, 517, 1236, 1282
LmnI GCTCC 2 cut(s) 146, 881
Lsp1109I GCAGC 4 cut(s) 50, 553, 1048, 1163
LweI GCATC 2 cut(s) 1062, 1474
MabI ACCWGGT 1 cut(s) 92
MaeI CTAG 1 cut(s) 1050
MaeII ACGT 1 cut(s) 918
MaeIII GTNAC 5 cut(s) 262, 349, 536, 949, 1385
MalI GATC 5 cut(s) 104, 274, 519, 1238, 1284
MboI GATC 5 cut(s) 102, 272, 517, 1236, 1282
MfeI CAATTG 3 cut(s) 339, 466, 1676
MhlI GDGCHC 1 cut(s) 1175
MlyI GAGTC 3 cut(s) 54, 698, 1382
MmeI TCCRAC 5 cut(s) 359, 369, 1109, 1474, 1571
Mph1103I ATGCAT 1 cut(s) 1744
MroXI GAANNNNTTC 1 cut(s) 1709
MseI TTAA 6 cut(s) 315, 381, 423, 1194, 1317, 1335
MslI CAYNNNNRTG 1 cut(s) 1470
MspA1I CMGCKG 1 cut(s) 635
MspR9I CCNGG 8 cut(s) 60, 94, 330, 927, 1120, 1156, 1326, 1368
MunI CAATTG 3 cut(s) 339, 466, 1676
MvaI CCWGG 8 cut(s) 60, 94, 330, 927, 1120, 1156, 1326, 1368
MvnI CGCG 1 cut(s) 72
MwoI GCNNNNNNNGC 5 cut(s) 63, 69, 1151, 1226, 1251
NdeII GATC 5 cut(s) 102, 272, 517, 1236, 1282
NlaIII CATG 4 cut(s) 258, 414, 444, 1415
NlaIV GGNNCC 5 cut(s) 463, 529, 1189, 1402, 1517
NmuCI GTSAC 3 cut(s) 349, 949, 1385
NsiI ATGCAT 1 cut(s) 1744
PaeR7I CTCGAG 1 cut(s) 42
PceI AGGCCT 1 cut(s) 1159
PcsI WCGNNNNNNNCGW 1 cut(s) 1425
PdmI GAANNNNTTC 1 cut(s) 1709
PfeI GAWTC 5 cut(s) 27, 475, 844, 908, 999
PflFI GACNNNGTC 2 cut(s) 1391, 1418
PkrI GCNGC 5 cut(s) 13, 65, 568, 1063, 1153
PleI GAGTC 3 cut(s) 53, 698, 1382
PpsI GAGTC 3 cut(s) 53, 698, 1382
PshBI ATTAAT 2 cut(s) 315, 381
PsiI TTATAA 1 cut(s) 743
Psp6I CCWGG 8 cut(s) 58, 92, 328, 925, 1118, 1154, 1324, 1366
PspEI GGTNACC 1 cut(s) 949
PspGI CCWGG 8 cut(s) 58, 92, 328, 925, 1118, 1154, 1324, 1366
PspN4I GGNNCC 5 cut(s) 463, 529, 1189, 1402, 1517
PspPI GGNCC 8 cut(s) 38, 56, 332, 1067, 1082, 1370, 1515, 1759
PspXI VCTCGAGB 1 cut(s) 42
PstI CTGCAG 1 cut(s) 1066
PstNI CAGNNNCTG 2 cut(s) 1373, 1553
PsyI GACNNNGTC 2 cut(s) 1391, 1418
RsaI GTAC 4 cut(s) 668, 1189, 1557, 1664
RsaNI GTAC 4 cut(s) 667, 1188, 1556, 1663
RseI CAYNNNNRTG 1 cut(s) 1470
SaqAI TTAA 6 cut(s) 315, 381, 423, 1194, 1317, 1335
SatI GCNGC 5 cut(s) 12, 64, 567, 1062, 1152
Sau3AI GATC 5 cut(s) 102, 272, 517, 1236, 1282
Sau96I GGNCC 8 cut(s) 38, 56, 332, 1067, 1082, 1370, 1515, 1759
SchI GAGTC 3 cut(s) 54, 698, 1382
ScrFI CCNGG 8 cut(s) 60, 94, 330, 927, 1120, 1156, 1326, 1368
SduI GDGCHC 1 cut(s) 1175
SexAI ACCWGGT 1 cut(s) 92
SfaNI GCATC 2 cut(s) 1062, 1474
SfcI CTRYAG 3 cut(s) 122, 936, 1062
Sfr274I CTCGAG 1 cut(s) 42
SinI GGWCC 4 cut(s) 38, 332, 1082, 1515
SlaI CTCGAG 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 1470
SmlI CTYRAG 3 cut(s) 42, 205, 1636
SmoI CTYRAG 3 cut(s) 42, 205, 1636
SseBI AGGCCT 1 cut(s) 1159
SsiI CCGC 3 cut(s) 12, 70, 633
SspMI CTAG 1 cut(s) 1050
StuI AGGCCT 1 cut(s) 1159
StyD4I CCNGG 8 cut(s) 58, 92, 328, 925, 1118, 1154, 1324, 1366
TaaI ACNGT 4 cut(s) 262, 649, 962, 1570
TaiI ACGT 1 cut(s) 921
TaqI TCGA 6 cut(s) 25, 43, 101, 298, 1036, 1422
TaqII GACCGA 2 cut(s) 26, 1381
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 5 cut(s) 27, 475, 844, 908, 999
Tru1I TTAA 6 cut(s) 315, 381, 423, 1194, 1317, 1335
Tru9I TTAA 6 cut(s) 315, 381, 423, 1194, 1317, 1335
TscAI CASTG 4 cut(s) 778, 1030, 1106, 1723
TseFI GTSAC 3 cut(s) 349, 949, 1385
TseI GCWGC 4 cut(s) 63, 566, 1061, 1151
Tsp45I GTSAC 3 cut(s) 349, 949, 1385
TspDTI ATGAA 7 cut(s) 17, 408, 548, 743, 911, 970, 1124
TspGWI ACGGA 1 cut(s) 285
TspRI CASTG 4 cut(s) 778, 1030, 1106, 1723
Tth111I GACNNNGTC 2 cut(s) 1391, 1418
VpaK11BI GGWCC 4 cut(s) 38, 332, 1082, 1515
VspI ATTAAT 2 cut(s) 315, 381
XagI CCTNNNNNAGG 1 cut(s) 1365
XapI RAATTY 3 cut(s) 797, 1029, 1135
XcmI CCANNNNNNNNNTGG 2 cut(s) 56, 489
XhoI CTCGAG 1 cut(s) 42
XmnI GAANNNNTTC 1 cut(s) 1709
XspI CTAG 1 cut(s) 1050
Zsp2I ATGCAT 1 cut(s) 1744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.