RLG00000017280

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010983
Physical Location & Seq
Reverse (-)
14913225 .. 14916071
2847 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017280

Sequence Viewer

Length: 1698 bp
ATGAAGTGCGCCGCAAATGAGCTTCGATTCTTCCTCGGTCCTCGCGTCCACGCTGGCCCTGGCTGCACCCGCGACCATTTATATAACCTCACCTGGTGTTCGATCACTCCTCACACTCAACTACAAAATCTGAAACTTGGGGGAATATATAGGAGCTTATACAATGAGGCCAACCTCTTTCCTGCGTTTTTTTCTTGGAACATTGCTTCTCCATTGGGTGTTCCTCGACAGGCTATCCTTATTAATGGTCAATTTCCTGGTCCAACAATTGAGGCTGTGACTAATGACAACATAATCGTTGACGTCATTAATAAGTTGGATGAACCTTTCCTCATTACATGGAATGGAATTAAACAGCGGAAGTCCTCATGGCAAGATGGAGTTCTCGGAACCAATTGCCCAATTCCTCCAAACACAAGCTGGAGATACAAGTTTCAAACAAAGGATCAGATTGGAACCTTCAGTTACTTCCCTTCAACGGAACTTCATAGAGCTGCTGGTGGTTTTGGGGGTTTCAATGTGGCACACAGGTCTGTGATAGCTGTCCCATATCCTATACCCGCTGAAGAGTTTACCATGCTTGTTGGTGATTGGTACAACACTAACCACAAGGTATTGCAACAAAAATTGGACTCTGGCAATCCTCTTCCTCTTCCTGATGGTCTTCTTATAAATGGGCTTCATAGGTCTGCAATCTTCACTGGTCAAGAAGGAAAGACCTACAAATTCAGGATATCAAATGTGGGAATAGCAACTTCAATCAACTTTAGGATTCAGGGTCATGTAATGACGCTTGTTGAAGTTGAAGGAGCTAATACCTTGCAAGAAGTGTATGAATCACTTGACGTTCATCCAGGCCAATCTGTAGCTGTTTTGGTTACCTTACACGGTTCAGTCAAGGACTATTTCATTGTGGCCTCTACCCGATTCACAAAGCCCATTCTCACAACTACTGGAATTCTTCGATATGCTGGTTCTAGCATCAAGGCTGCAGGGCCATTGCCTGTTGGTCCAACCTATCACATTCACTGGTCTATGAAGCAAGCCAGGACAATCAGATTGAATTTGACAGCAAATGCAGCCAGGCCTAACCCTCAGGGCTCATTCCATTATGGTACCATTAAAGTAGTGAGGACACTTGTTTTAGCCAATTCTGCTACTAAGATCGGTGGCAAGCTTCGCTATGCTGTAAACAAGATATCATATGTTGATCCAGAGACCCCCTTGAAGCTGGCTGACTGGTTTAACATCCCTGGAGTATTTAACTTCAACACAATCAAGGACACACCTACTCCAGGCCCTGCAAGTTTTGGTGCCTCGGTCATAGGAACCACACTCCATGACTTCGTTGAAATCGTATTCCAGAACACCGAACCCACACTTCAATCTTGGCATCTTGATGGATATAGCTTCTACATTGTTGGATATGGTTCTAAAAATTGGACTCCAGATATGAGGAGACGCTACAATTTGGCAGACGCTGTACCAAGACACACAGTTCAGGTATATCCATTTGGTTGGAGTGCTGTGATGGTGTCTTTGGACAACAAGGGTATGTGGAACTTGAGGTCTGCAATCTGGTCAAGAAGGTACTTGGGTCAGCAATTGTATATCAGAGTTTGGAACGATGAGAAAAGCCTCCTCACTGAGAATGATATCCCTCAAAATGCATTATTTTGTGGCAAGGCCCATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

566

Amino Acids

63.08

Weight (kDa)

9.4

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 68 - 177 5.2e-39 Multicopper oxidase
Cu-oxidase PF00394 189 - 325 4.3e-38 Multicopper oxidase
Cu-oxidase_2 PF07731 407 - 545 1.6e-31 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010983)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23630 AT2G23630 AT4G37160 AT4G37160
fragaria_vesca FvH4_1g14980 FvH4_1g14980
malus_domestica MD02G1163600.v1.1
prunus_persica Prupe.7G140600_v2.0.a1 Prupe.7G140600_v2.0.a1
pyrus_communis pycom02g13000
rosa_chinensis RchiOBHm_Chr2g0104041
rosa_laevigata RLG00000017280
rosa_multiflora Rmu_sc0000193.1_g000006
rosa_roxburghii Rroxscaffold_2G00139180
rosa_rugosa Rorug02G0119000
rosa_samantha Rh2AG168100 Rh2BG175100 Rh2CG174000 Rh2DG173600
rosa_wichuraiana Rw2G013150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 671
AatII GACGTC 1 cut(s) 306
Acc65I GGTACC 1 cut(s) 1115
AccB1I GGYRCC 2 cut(s) 1115, 1313
AccII CGCG 2 cut(s) 45, 72
AciI CCGC 4 cut(s) 12, 70, 358, 561
AclWI GGATC 2 cut(s) 453, 1205
AcsI RAATTY 3 cut(s) 725, 957, 1063
AcuI CTGAAG 2 cut(s) 445, 585
AcyI GRCGYC 1 cut(s) 303
AdeI CACNNNGTG 1 cut(s) 96
AfaI GTAC 4 cut(s) 596, 1117, 1485, 1592
AfiI CCNNNNNNNGG 2 cut(s) 478, 1295
AjnI CCWGG 8 cut(s) 58, 92, 256, 853, 1046, 1082, 1252, 1294
Alw26I GTCTC 2 cut(s) 1211, 1453
AlwI GGATC 2 cut(s) 453, 1205
AlwNI CAGNNNCTG 2 cut(s) 1301, 1481
AoxI GGCC 8 cut(s) 55, 168, 856, 915, 995, 1085, 1297, 1686
ApeKI GCWGC 4 cut(s) 63, 494, 989, 1079
ApoI RAATTY 3 cut(s) 725, 957, 1063
AseI ATTAAT 2 cut(s) 243, 309
Asp718I GGTACC 1 cut(s) 1115
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 7 cut(s) 38, 56, 260, 995, 1010, 1298, 1687
AsuHPI GGTGA 2 cut(s) 82, 599
AvaII GGWCC 3 cut(s) 38, 260, 1010
AxyI CCTNAGG 1 cut(s) 1095
BaeI ACNNNNGTAYC 2 cut(s) 1582, 1615
BanI GGYRCC 2 cut(s) 1115, 1313
BanII GRGCYC 1 cut(s) 1103
BbsI GAAGAC 1 cut(s) 656
BbvI GCAGC 4 cut(s) 50, 481, 976, 1091
BccI CCATC 4 cut(s) 371, 653, 1394, 1525
BciT130I CCWGG 8 cut(s) 60, 94, 258, 855, 1048, 1084, 1254, 1296
BcoDI GTCTC 2 cut(s) 1211, 1453
BfaI CTAG 1 cut(s) 978
BfmI CTRYAG 2 cut(s) 864, 990
BisI GCNGC 5 cut(s) 12, 64, 495, 990, 1080
BlsI GCNGC 5 cut(s) 13, 65, 496, 991, 1081
Bme1390I CCNGG 8 cut(s) 60, 94, 258, 855, 1048, 1084, 1254, 1296
Bme18I GGWCC 3 cut(s) 38, 260, 1010
BmgT120I GGNCC 7 cut(s) 38, 56, 260, 995, 1010, 1298, 1687
BmiI GGNNCC 5 cut(s) 391, 457, 1117, 1315, 1330
BmrFI CCNGG 8 cut(s) 60, 94, 258, 855, 1048, 1084, 1254, 1296
BmsI GCATC 2 cut(s) 990, 1402
BpiI GAAGAC 1 cut(s) 656
BpmI CTGGAG 4 cut(s) 442, 1275, 1278, 1431
BpuEI CTTGAG 1 cut(s) 1585
BsaHI GRCGYC 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 1211
BsaJI CCNNGG 4 cut(s) 34, 58, 1252, 1317
BsaXI ACNNNNNCTCC 2 cut(s) 1276, 1306
Bsc4I CCNNNNNNNGG 2 cut(s) 478, 1295
Bse1I ACTGG 4 cut(s) 706, 958, 1034, 1244
Bse21I CCTNAGG 1 cut(s) 1095
Bse3DI GCAATG 2 cut(s) 201, 998
BseBI CCWGG 8 cut(s) 60, 94, 258, 855, 1048, 1084, 1254, 1296
BseDI CCNNGG 4 cut(s) 34, 58, 1252, 1317
BseGI GGATG 3 cut(s) 325, 850, 1248
BseLI CCNNNNNNNGG 2 cut(s) 478, 1295
BseMI GCAATG 2 cut(s) 201, 998
BseMII CTCAG 2 cut(s) 1109, 1638
BseNI ACTGG 4 cut(s) 706, 958, 1034, 1244
BseRI GAGGAG 3 cut(s) 99, 1471, 1631
BseXI GCAGC 4 cut(s) 50, 481, 976, 1091
BsgI GTGCAG 1 cut(s) 49
Bsh1236I CGCG 2 cut(s) 45, 72
BshFI GGCC 8 cut(s) 57, 170, 858, 917, 997, 1087, 1299, 1688
BshNI GGYRCC 2 cut(s) 1115, 1313
BslFI GGGAC 1 cut(s) 530
BslI CCNNNNNNNGG 2 cut(s) 478, 1295
BsmAI GTCTC 2 cut(s) 1211, 1453
BsmBI CGTCTC 1 cut(s) 1453
BsmFI GGGAC 1 cut(s) 530
BsnI GGCC 8 cut(s) 57, 170, 858, 917, 997, 1087, 1299, 1688
Bso31I GGTCTC 1 cut(s) 1211
Bsp1286I GDGCHC 1 cut(s) 1103
Bsp143I GATC 4 cut(s) 102, 445, 1164, 1210
BspACI CCGC 4 cut(s) 12, 70, 358, 561
BspANI GGCC 8 cut(s) 57, 170, 858, 917, 997, 1087, 1299, 1688
BspCNI CTCAG 2 cut(s) 1108, 1639
BspFNI CGCG 2 cut(s) 45, 72
BspLI GGNNCC 5 cut(s) 391, 457, 1117, 1315, 1330
BspMAI CTGCAG 1 cut(s) 994
BspPI GGATC 2 cut(s) 453, 1205
BspT107I GGYRCC 2 cut(s) 1115, 1313
BspTNI GGTCTC 1 cut(s) 1211
BsrDI GCAATG 2 cut(s) 201, 998
BsrI ACTGG 4 cut(s) 706, 958, 1034, 1244
BssECI CCNNGG 4 cut(s) 34, 58, 1252, 1317
BssMI GATC 4 cut(s) 102, 445, 1164, 1210
BssNI GRCGYC 1 cut(s) 303
Bst2UI CCWGG 8 cut(s) 60, 94, 258, 855, 1048, 1084, 1254, 1296
Bst4CI ACNGT 2 cut(s) 890, 1498
Bst6I CTCTTC 3 cut(s) 561, 651, 657
BstACI GRCGYC 1 cut(s) 303
BstC8I GCNNGC 4 cut(s) 55, 1044, 1175, 1233
BstDEI CTNAG 3 cut(s) 1095, 1161, 1647
BstEII GGTNACC 1 cut(s) 877
BstENI CCTNNNNNAGG 1 cut(s) 1293
BstF5I GGATG 3 cut(s) 325, 850, 1248
BstFNI CGCG 2 cut(s) 45, 72
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 4 cut(s) 105, 448, 1167, 1213
BstMAI GTCTC 2 cut(s) 1211, 1453
BstMBI GATC 4 cut(s) 102, 445, 1164, 1210
BstMWI GCNNNNNNNGC 5 cut(s) 63, 69, 1079, 1154, 1179
BstNI CCWGG 8 cut(s) 60, 94, 258, 855, 1048, 1084, 1254, 1296
BstPI GGTNACC 1 cut(s) 877
BstSCI CCNGG 8 cut(s) 58, 92, 256, 853, 1046, 1082, 1252, 1294
BstSFI CTRYAG 2 cut(s) 864, 990
BstUI CGCG 2 cut(s) 45, 72
BstV1I GCAGC 4 cut(s) 50, 481, 976, 1091
BstV2I GAAGAC 1 cut(s) 656
BstXI CCANNNNNNTGG 1 cut(s) 1518
Bsu36I CCTNAGG 1 cut(s) 1095
BsuRI GGCC 8 cut(s) 57, 170, 858, 917, 997, 1087, 1299, 1688
BtsCI GGATG 3 cut(s) 325, 850, 1248
BtsIMutI CAGTG 4 cut(s) 699, 1027, 1644, 1693
Cac8I GCNNGC 4 cut(s) 55, 1044, 1175, 1233
CaiI CAGNNNCTG 2 cut(s) 1301, 1481
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 7 cut(s) 38, 56, 260, 995, 1010, 1298, 1687
CseI GACGC 4 cut(s) 34, 799, 1470, 1487
CsiI ACCWGGT 1 cut(s) 92
Csp6I GTAC 4 cut(s) 595, 1116, 1484, 1591
CviAII CATG 5 cut(s) 339, 369, 577, 782, 1340
CviQI GTAC 4 cut(s) 595, 1116, 1484, 1591
DdeI CTNAG 3 cut(s) 1095, 1161, 1647
DpnI GATC 4 cut(s) 104, 447, 1166, 1212
DpnII GATC 4 cut(s) 102, 445, 1164, 1210
DraIII CACNNNGTG 1 cut(s) 96
Eam1104I CTCTTC 3 cut(s) 561, 651, 657
EarI CTCTTC 3 cut(s) 561, 651, 657
Eco147I AGGCCT 1 cut(s) 1087
Eco24I GRGCYC 1 cut(s) 1103
Eco31I GGTCTC 1 cut(s) 1211
Eco32I GATATC 3 cut(s) 735, 1200, 1657
Eco47I GGWCC 3 cut(s) 38, 260, 1010
Eco57I CTGAAG 2 cut(s) 445, 585
Eco81I CCTNAGG 1 cut(s) 1095
Eco91I GGTNACC 1 cut(s) 877
EcoNI CCTNNNNNAGG 1 cut(s) 1293
EcoO109I RGGNCCY 1 cut(s) 1298
EcoO65I GGTNACC 1 cut(s) 877
EcoRI GAATTC 1 cut(s) 957
EcoRII CCWGG 8 cut(s) 58, 92, 256, 853, 1046, 1082, 1252, 1294
EcoRV GATATC 3 cut(s) 735, 1200, 1657
EcoT22I ATGCAT 1 cut(s) 1672
EcoT38I GRGCYC 1 cut(s) 1103
Esp3I CGTCTC 1 cut(s) 1453
FaeI CATG 5 cut(s) 342, 372, 580, 785, 1343
FaqI GGGAC 1 cut(s) 530
FatI CATG 5 cut(s) 338, 368, 576, 781, 1339
FauI CCCGC 2 cut(s) 77, 568
FauNDI CATATG 1 cut(s) 1204
Fnu4HI GCNGC 5 cut(s) 12, 64, 495, 990, 1080
FokI GGATG 3 cut(s) 332, 837, 1235
FriOI GRGCYC 1 cut(s) 1103
Fsp4HI GCNGC 5 cut(s) 12, 64, 495, 990, 1080
FspBI CTAG 1 cut(s) 978
GlaI GCGC 1 cut(s) 10
GluI GCNGC 5 cut(s) 12, 64, 495, 990, 1080
GsuI CTGGAG 4 cut(s) 442, 1275, 1278, 1431
HaeIII GGCC 8 cut(s) 57, 170, 858, 917, 997, 1087, 1299, 1688
HgaI GACGC 4 cut(s) 34, 799, 1470, 1487
HhaI GCGC 1 cut(s) 11
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 5 cut(s) 342, 372, 580, 785, 1343
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HindIII AAGCTT 1 cut(s) 1175
HinfI GANTC 6 cut(s) 27, 632, 772, 836, 927, 1444
HphI GGTGA 2 cut(s) 82, 599
Hpy166II GTNNAC 4 cut(s) 49, 301, 573, 1192
Hpy188I TCNGA 5 cut(s) 132, 389, 450, 1058, 1616
Hpy188III TCNNGA 8 cut(s) 656, 707, 730, 1214, 1363, 1397, 1448, 1584
Hpy8I GTNNAC 4 cut(s) 49, 301, 573, 1192
HpyAV CCTTC 5 cut(s) 469, 483, 704, 800, 1581
HpyCH4III ACNGT 2 cut(s) 890, 1498
HpyCH4IV ACGT 2 cut(s) 303, 846
HpyCH4V TGCA 9 cut(s) 66, 619, 692, 823, 992, 1079, 1304, 1574, 1670
HpyF10VI GCNNNNNNNGC 5 cut(s) 63, 69, 1079, 1154, 1179
HpyF3I CTNAG 3 cut(s) 1095, 1161, 1647
HpySE526I ACGT 2 cut(s) 303, 846
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 5 cut(s) 342, 372, 580, 785, 1343
HspAI GCGC 1 cut(s) 9
KpnI GGTACC 1 cut(s) 1119
Kzo9I GATC 4 cut(s) 102, 445, 1164, 1210
LmnI GCTCC 2 cut(s) 153, 809
Lsp1109I GCAGC 4 cut(s) 50, 481, 976, 1091
LweI GCATC 2 cut(s) 990, 1402
MabI ACCWGGT 1 cut(s) 92
MaeI CTAG 1 cut(s) 978
MaeII ACGT 2 cut(s) 303, 846
MaeIII GTNAC 3 cut(s) 277, 464, 877
MalI GATC 4 cut(s) 104, 447, 1166, 1212
MboI GATC 4 cut(s) 102, 445, 1164, 1210
MboII GAAGA 7 cut(s) 22, 578, 638, 644, 656, 688, 953
MfeI CAATTG 3 cut(s) 267, 394, 1604
MhlI GDGCHC 1 cut(s) 1103
MlyI GAGTC 2 cut(s) 626, 1438
MmeI TCCRAC 5 cut(s) 287, 297, 1037, 1402, 1499
Mph1103I ATGCAT 1 cut(s) 1672
MseI TTAA 6 cut(s) 243, 309, 351, 1122, 1245, 1263
MslI CAYNNNNRTG 1 cut(s) 1398
MspA1I CMGCKG 2 cut(s) 358, 563
MspR9I CCNGG 8 cut(s) 60, 94, 258, 855, 1048, 1084, 1254, 1296
MunI CAATTG 3 cut(s) 267, 394, 1604
MvaI CCWGG 8 cut(s) 60, 94, 258, 855, 1048, 1084, 1254, 1296
MvnI CGCG 2 cut(s) 45, 72
MwoI GCNNNNNNNGC 5 cut(s) 63, 69, 1079, 1154, 1179
NdeI CATATG 1 cut(s) 1204
NdeII GATC 4 cut(s) 102, 445, 1164, 1210
NlaIII CATG 5 cut(s) 342, 372, 580, 785, 1343
NlaIV GGNNCC 5 cut(s) 391, 457, 1117, 1315, 1330
NmuCI GTSAC 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 1672
PceI AGGCCT 1 cut(s) 1087
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 1353
PfeI GAWTC 4 cut(s) 27, 772, 836, 927
PkrI GCNGC 5 cut(s) 13, 65, 496, 991, 1081
PleI GAGTC 2 cut(s) 626, 1438
PpsI GAGTC 2 cut(s) 626, 1438
PshBI ATTAAT 2 cut(s) 243, 309
PsiI TTATAA 1 cut(s) 671
Psp6I CCWGG 8 cut(s) 58, 92, 256, 853, 1046, 1082, 1252, 1294
PspEI GGTNACC 1 cut(s) 877
PspGI CCWGG 8 cut(s) 58, 92, 256, 853, 1046, 1082, 1252, 1294
PspN4I GGNNCC 5 cut(s) 391, 457, 1117, 1315, 1330
PspPI GGNCC 7 cut(s) 38, 56, 260, 995, 1010, 1298, 1687
PstI CTGCAG 1 cut(s) 994
PstNI CAGNNNCTG 2 cut(s) 1301, 1481
RsaI GTAC 4 cut(s) 596, 1117, 1485, 1592
RsaNI GTAC 4 cut(s) 595, 1116, 1484, 1591
RseI CAYNNNNRTG 1 cut(s) 1398
SaqAI TTAA 6 cut(s) 243, 309, 351, 1122, 1245, 1263
SatI GCNGC 5 cut(s) 12, 64, 495, 990, 1080
Sau3AI GATC 4 cut(s) 102, 445, 1164, 1210
Sau96I GGNCC 7 cut(s) 38, 56, 260, 995, 1010, 1298, 1687
SchI GAGTC 2 cut(s) 626, 1438
ScrFI CCNGG 8 cut(s) 60, 94, 258, 855, 1048, 1084, 1254, 1296
SduI GDGCHC 1 cut(s) 1103
SexAI ACCWGGT 1 cut(s) 92
SfaNI GCATC 2 cut(s) 990, 1402
SfcI CTRYAG 2 cut(s) 864, 990
SinI GGWCC 3 cut(s) 38, 260, 1010
SmiMI CAYNNNNRTG 1 cut(s) 1398
SmlI CTYRAG 1 cut(s) 1564
SmoI CTYRAG 1 cut(s) 1564
SseBI AGGCCT 1 cut(s) 1087
SsiI CCGC 4 cut(s) 12, 70, 358, 561
SspMI CTAG 1 cut(s) 978
StuI AGGCCT 1 cut(s) 1087
StyD4I CCNGG 8 cut(s) 58, 92, 256, 853, 1046, 1082, 1252, 1294
TaaI ACNGT 2 cut(s) 890, 1498
TaiI ACGT 2 cut(s) 306, 849
TaqI TCGA 4 cut(s) 25, 101, 226, 964
TaqII GACCGA 2 cut(s) 26, 1309
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 4 cut(s) 27, 772, 836, 927
Tru1I TTAA 6 cut(s) 243, 309, 351, 1122, 1245, 1263
Tru9I TTAA 6 cut(s) 243, 309, 351, 1122, 1245, 1263
TscAI CASTG 3 cut(s) 706, 1034, 1651
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 4 cut(s) 63, 494, 989, 1079
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 8 cut(s) 17, 336, 476, 671, 839, 849, 898, 1052
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 3 cut(s) 706, 1034, 1651
VpaK11BI GGWCC 3 cut(s) 38, 260, 1010
VspI ATTAAT 2 cut(s) 243, 309
XagI CCTNNNNNAGG 1 cut(s) 1293
XapI RAATTY 3 cut(s) 725, 957, 1063
XcmI CCANNNNNNNNNTGG 2 cut(s) 56, 417
XspI CTAG 1 cut(s) 978
ZraI GACGTC 1 cut(s) 304
Zsp2I ATGCAT 1 cut(s) 1672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.