FvH4_1g16260

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009363
Physical Location & Seq
Reverse (-)
9323522 .. 9330791
7270 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16260.t1

Sequence Viewer

Length: 2724 bp
ATGGCGTCGAAGGCTTCGCCTTCCGCCTCTGCTAACAGATCGGAGCCTCACCAGGATCCTCCGATGACGGGAACAGAAGAAACTGCTATGACCAGGGACCAAAAGGAGATTTCATTGATTATAGATTCTCTGCAGAAGCAAGTTATTTCTAACCGCGGTGTTTCTATAAAGAAAAGAATGGAAGAAAACAGGCAGAAATTGGCTGCTGTCACTAACAATTTTTATAACTTGTCATTGGAAAGAAGAAACAATAGCTTATTAAATCCTGGTAAAAGTGTTGACCTATTGACAAAGAGGCAACAAGATGCATTTAAGCTGCAAAATGGTGATGAAAGTAATGGGAGCAATGATAGCTCTCAAGAAGATGGGCGTGGTTCCACAGCAGTTCTTCTGGAATCTAATGTTGCTGTCAAGAATGCTGTTCGCCCTATCAAGCTTCCGGAAGTTAAAAGACTACCTCCTTATACTACATGGGTATTTTTGGACAGAAATCAAAGAATGACTGAGGATCAGTCGATTTTGGGTCGAAGAAGAATTTATTATGATCAAAATGGAGGTGAAGCTCTTATATGTAGTGATAGTGAGGAGGAAGCAATTGATGAAGAAGAGGAAAAGAGGGAGTTTGTTGAATCTGAAGACTATATACTTCGCATGACTATAAAAGAAGCTGGTGTCTCCGATCCTGTGCTGGAGTCATTGGCACATTATTTCTCCAGAAGCCCTACTGAAGTCAAGGCAAGATATGAAGCTCTTATAAAAGAAGAGGAGTCCATGGGGATTTCTAAGAATACGGATGACGAAGACCTTTCAAAAAATGACAATTCTTTTCTTGATAAAGATCTGGATGCAGCCCTTGATTCATTTGATAACCTATTTTGTCGGCGATGCCTTGTATTTGATTGCAGATTGCATGGATGTTCACAGGATCTTGTCTTTCCTGCCGAGAAACACGCTCCTCGGAGTCCACCAGATGATGAAAATTTGCCATGTGGTCCAAATTGTTATAGATCGGTACGGAAGTCTGAAGGTACAGTAAGCTCTCCGATGAATGACTATCTGGAAGAGAAAGGTAGCAAGAGATCTTCTGGTTTATCTAGAAAGAAGGGAAAGTCCTTTCAAAGTGAAAGTGCTTCATCAAATGCAAAAAACATATCAGAAAGCAGTGATTCGGAGAATGAAACCAGGCAGGATGATACTTGTAGCCATGATCCAATATCCTCAAAGACTAAGGTAGCAGGGAAAATTGGAACCCCTAAGAGGAACAGCAAGAGAGTTGCAGAGCGTGTTCTGGTTTGTATGCAGAAGAGGCAGAAGAAAACAATGGCTTCTGATTCTGATTCAATAGTTGATGGTGGTCTTTGTGCAAGTGATACAAAGCTTAGATCTAATTCATGCAAAGATAATGAAGACACTAGTTCGTCTTCGCAAAAGAATCTAAAATCTTCTACTAGTGGAAGGTCTAGGAGGGAATCACCTTTGAAGGACAGTAACAAAGTAGTTCAGGGTGAAGTTGTTGATGGCTCATTAAATGAGATGATCACTGATCCACCTGCCACTAGTAGTGATGACAATTTGAGAAAAGAAGAGCATGTAGATGAAAACATATACAAGCAAGAATGCAGTGATGACAAAACTTGGAAAACTATTGAAACAAGTCTTTTCGAGAAAGGCATAGAGATATTTGGCAAGAACAGCTGTTTAATAGCAAGGAACTTGTTAAATGGCATGAAAACTTGTTGGGAAGTCTTTCAGTATATGAATTACTCGGACAGTAAGGTTTCCTGCCAAGCAGGTGATGCTGCAAACTCCCTTGTTGAAGGGTATTCCAAGGGTAATAATGAAGCAAGAAGAAGATCAAAATTTTTACGTAGGAAGGGTAGAGTTCGTCGCTTGAAGTACACTTGGAAGTCTGCTGCTTACCATTCGATCAGGAAACGAATTACTGATAAAAAAGATCAACCATGCAGGCAGTATAATCCATGTACTTGCCAAACAGCTTGTGGAAAGCAGTGTAGTTGTCTTCTAAATGGGACATGTTGTGAGAAGTACTGTGGGTGTCCTAAGAGTTGCAAGAATAGATTTAGAGGCTGTCATTGTGCTAAAAGTCAATGCCGAAGTCGTCAATGCCCATGCTTTGCTGCAGATAGGGAATGTGATCCAGATGTTTGTAGGAATTGCTGGGTCAGTTGTGGTGATGGTAGTCTTGGGGTTCCGAACCAAAGAGGTGATAATTATGAGTGTAGAAATATGAAGCTTCTTCTGAAACAACAACAAAGGGTCTTACTTGGAAGATCTGATGTATCTGGCTGGGGTGCTTTCTTAAAGAATAGTGTCAGCAAGCATGAATATCTTGGTGAGTATACTGGGGAGTTGATCTCACATCGTGAAGCAGATAAGCGTGGAAAAATATACGACCGTGAAAATTCATCATTTCTCTTCAATCTGAATGATCAGTTTGTTCTTGATGCCTATCGGAAGGGTGATAAATTGAAGTTTGCCAACCACTCTCCAGATCCAAATTGCTATGCAAAGGTCATTATGGTTGCTGGAGATCACAGGGTGGGTATATTTGCCAAGGAACGAATTGCTTCGGGAGAGGAACTTTTCTATGATTACCGTTATGAGCCAGATAGAGCTCCTGCTTGGGCTCGGAAGCCTGAGGCATCCGGGTCAAAAAGAGACGACACAGCCCCGTCAAGTGGTCGCGCAAAGAAGCTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003700 GO:0003723 GO:0003727 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005677 GO:0006139 GO:0006325 GO:0006342 GO:0006349 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009292 GO:0009294 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009965 GO:0009987 GO:0010016 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016278 GO:0016279 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0017053 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031519 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0040030 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044764 GO:0045814 GO:0045857 GO:0045892 GO:0045934 GO:0046483 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048586 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051704 GO:0060255 GO:0061458 GO:0065007 GO:0065009 GO:0070013 GO:0070734 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090568 GO:0097159 GO:0099402 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1905392 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00310 ko05206 map00310 map05206
Pfam Domains
Protein Families
SET

Protein Analysis

908

Amino Acids

101.99

Weight (kDa)

8.45

Isoelectric Point (pI)

55.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_CLF_N PF25996 208 - 251 1.4e-19 Histone-lysine N-methyltransferase CLF, N-terminal HTH domain
preSET_CXC PF18264 696 - 727 8.6e-08 CXC domain
SET PF00856 769 - 872 2.7e-19 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 225, 755
AarI CACCTGC 2 cut(s) 1558, 1781
Acc36I ACCTGC 2 cut(s) 1558, 1781
AccI GTMKAC 1 cut(s) 2360
AccII CGCG 2 cut(s) 156, 2706
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 3 cut(s) 24, 154, 156
AclWI GGATC 9 cut(s) 50, 63, 516, 674, 933, 1202, 1538, 2150, 2507
AcsI RAATTY 4 cut(s) 534, 979, 1859, 2422
AcuI CTGAAG 3 cut(s) 654, 747, 1044
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 2 cut(s) 2384, 2560
AfaI GTAC 5 cut(s) 1014, 1030, 1898, 1984, 2048
AfiI CCNNNNNNNGG 3 cut(s) 68, 1257, 2699
AflIII ACRYGT 1 cut(s) 2033
AhlI ACTAGT 3 cut(s) 1412, 1448, 1556
AjnI CCWGG 4 cut(s) 51, 92, 265, 1181
Alw21I GWGCWC 1 cut(s) 2638
Alw26I GTCTC 2 cut(s) 679, 2673
AlwI GGATC 9 cut(s) 50, 63, 516, 674, 933, 1202, 1538, 2150, 2507
Aor13HI TCCGGA 1 cut(s) 439
ApeKI GCWGC 6 cut(s) 203, 316, 848, 1799, 1913, 2138
ApoI RAATTY 4 cut(s) 534, 979, 1859, 2422
Asp700I GAANNNNTTC 2 cut(s) 1746, 2587
AspLEI GCGC 1 cut(s) 2708
AspS9I GGNCC 2 cut(s) 97, 992
AsuC2I CCSGG 1 cut(s) 2668
AvaII GGWCC 2 cut(s) 97, 992
AxyI CCTNAGG 1 cut(s) 2658
BaeI ACNNNNGTAYC 2 cut(s) 1004, 1037
BamHI GGATCC 1 cut(s) 55
BanII GRGCYC 2 cut(s) 2638, 2650
BarI GAAGNNNNNNTAC 2 cut(s) 627, 659
BbsI GAAGAC 5 cut(s) 642, 807, 1413, 1413, 2012
Bbv12I GWGCWC 1 cut(s) 2638
BbvI GCAGC 6 cut(s) 190, 303, 860, 1786, 1900, 2125
BccI CCATC 4 cut(s) 359, 1343, 1511, 2189
BciT130I CCWGG 4 cut(s) 53, 94, 267, 1183
BclI TGATCA 3 cut(s) 544, 1536, 2449
BcnI CCSGG 1 cut(s) 2668
BcoDI GTCTC 2 cut(s) 679, 2673
BcuI ACTAGT 3 cut(s) 1412, 1448, 1556
BfaI CTAG 5 cut(s) 1095, 1413, 1449, 1461, 1557
BfmI CTRYAG 2 cut(s) 131, 2139
BfuAI ACCTGC 2 cut(s) 1558, 1781
BglII AGATCT 4 cut(s) 838, 1079, 1382, 2291
BisI GCNGC 6 cut(s) 204, 317, 849, 1800, 1914, 2139
BlsI GCNGC 6 cut(s) 205, 318, 850, 1801, 1915, 2140
BmcAI AGTACT 1 cut(s) 2048
Bme1390I CCNGG 5 cut(s) 53, 94, 267, 1183, 2668
Bme18I GGWCC 2 cut(s) 97, 992
BmgT120I GGNCC 2 cut(s) 97, 992
BmiI GGNNCC 6 cut(s) 45, 57, 98, 376, 1249, 2211
BmrFI CCNGG 5 cut(s) 53, 94, 267, 1183, 2668
BmrI ACTGGG 1 cut(s) 2373
BmsI GCATC 6 cut(s) 295, 835, 875, 1786, 2455, 2672
BmuI ACTGGG 1 cut(s) 2373
BpiI GAAGAC 5 cut(s) 642, 807, 1413, 1413, 2012
BplI GAGNNNNNCTC 2 cut(s) 2360, 2392
BpmI CTGGAG 4 cut(s) 697, 710, 2493, 2568
BpuEI CTTGAG 1 cut(s) 342
BpuMI CCSGG 1 cut(s) 2668
BsaAI YACGTR 1 cut(s) 1868
BsaBI GATNNNNATC 2 cut(s) 837, 2469
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 6 cut(s) 93, 154, 771, 956, 1827, 2574
BsaWI WCCGGW 1 cut(s) 439
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 1257, 2699
Bse1I ACTGG 1 cut(s) 2368
Bse21I CCTNAGG 1 cut(s) 2658
Bse3DI GCAATG 1 cut(s) 352
Bse8I GATNNNNATC 2 cut(s) 837, 2469
BseAI TCCGGA 1 cut(s) 439
BseBI CCWGG 4 cut(s) 53, 94, 267, 1183
BseDI CCNNGG 6 cut(s) 93, 154, 771, 956, 1827, 2574
BseGI GGATG 5 cut(s) 799, 850, 920, 1195, 2663
BseJI GATNNNNATC 2 cut(s) 837, 2469
BseLI CCNNNNNNNGG 3 cut(s) 68, 1257, 2699
BseMI GCAATG 1 cut(s) 352
BseMII CTCAG 2 cut(s) 495, 2649
BseNI ACTGG 1 cut(s) 2368
BseRI GAGGAG 3 cut(s) 599, 779, 945
BseXI GCAGC 6 cut(s) 190, 303, 860, 1786, 1900, 2125
BseYI CCCAGC 2 cut(s) 2178, 2307
Bsh1236I CGCG 2 cut(s) 156, 2706
Bsh1285I CGRYCG 1 cut(s) 2416
BsiEI CGRYCG 1 cut(s) 2416
BsiHKAI GWGCWC 1 cut(s) 2638
BsiSI CCGG 2 cut(s) 440, 2667
BslFI GGGAC 2 cut(s) 110, 2044
BslI CCNNNNNNNGG 3 cut(s) 68, 1257, 2699
BsmAI GTCTC 2 cut(s) 679, 2673
BsmBI CGTCTC 1 cut(s) 2673
BsmFI GGGAC 2 cut(s) 110, 2044
BsmI GAATGC 2 cut(s) 421, 1622
Bsp1286I GDGCHC 2 cut(s) 2638, 2650
Bsp13I TCCGGA 1 cut(s) 439
Bsp19I CCATGG 1 cut(s) 771
BspACI CCGC 3 cut(s) 24, 154, 156
BspCNI CTCAG 2 cut(s) 496, 2650
BspEI TCCGGA 1 cut(s) 439
BspFNI CGCG 2 cut(s) 156, 2706
BspLI GGNNCC 6 cut(s) 45, 57, 98, 376, 1249, 2211
BspMAI CTGCAG 2 cut(s) 135, 2143
BspMI ACCTGC 2 cut(s) 1558, 1781
BspPI GGATC 9 cut(s) 50, 63, 516, 674, 933, 1202, 1538, 2150, 2507
BspQI GCTCTTC 1 cut(s) 1578
BsrDI GCAATG 1 cut(s) 352
BsrI ACTGG 1 cut(s) 2368
BssECI CCNNGG 6 cut(s) 93, 154, 771, 956, 1827, 2574
BssNAI GTATAC 1 cut(s) 2361
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 771, 1827, 2574
Bst1107I GTATAC 1 cut(s) 2361
Bst2UI CCWGG 4 cut(s) 53, 94, 267, 1183
Bst4CI ACNGT 6 cut(s) 1033, 1487, 1772, 2051, 2417, 2618
Bst6I CTCTTC 6 cut(s) 600, 756, 1056, 1298, 1578, 2441
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1796
BstBAI YACGTR 1 cut(s) 1868
BstC8I GCNNGC 3 cut(s) 1967, 2339, 2718
BstDEI CTNAG 7 cut(s) 504, 783, 1227, 1254, 1379, 2061, 2658
BstDSI CCRYGG 2 cut(s) 154, 771
BstF5I GGATG 5 cut(s) 799, 850, 920, 1195, 2663
BstFNI CGCG 2 cut(s) 156, 2706
BstHHI GCGC 1 cut(s) 2708
BstMAI GTCTC 2 cut(s) 679, 2673
BstMCI CGRYCG 1 cut(s) 2416
BstMWI GCNNNNNNNGC 5 cut(s) 11, 351, 1306, 1692, 1796
BstNI CCWGG 4 cut(s) 53, 94, 267, 1183
BstNSI RCATGY 2 cut(s) 1592, 2037
BstSCI CCNGG 5 cut(s) 51, 92, 265, 1181, 2666
BstSFI CTRYAG 2 cut(s) 131, 2139
BstSNI TACGTA 1 cut(s) 1868
BstUI CGCG 2 cut(s) 156, 2706
BstV1I GCAGC 6 cut(s) 190, 303, 860, 1786, 1900, 2125
BstV2I GAAGAC 5 cut(s) 642, 807, 1413, 1413, 2012
BstX2I RGATCY 7 cut(s) 55, 838, 925, 1079, 1382, 2291, 2512
BstYI RGATCY 7 cut(s) 55, 838, 925, 1079, 1382, 2291, 2512
BstZ17I GTATAC 1 cut(s) 2361
Bsu36I CCTNAGG 1 cut(s) 2658
BtgI CCRYGG 2 cut(s) 154, 771
BtgZI GCGATG 1 cut(s) 898
BtsCI GGATG 5 cut(s) 799, 850, 920, 1195, 2663
BtsI GCAGTG 3 cut(s) 1168, 1627, 2015
BtsIMutI CAGTG 4 cut(s) 1168, 1539, 1627, 2015
BveI ACCTGC 2 cut(s) 1558, 1781
Cac8I GCNNGC 3 cut(s) 1967, 2339, 2718
CfoI GCGC 1 cut(s) 2708
Cfr13I GGNCC 2 cut(s) 97, 992
Cfr42I CCGCGG 1 cut(s) 157
Csp6I GTAC 5 cut(s) 1013, 1029, 1897, 1983, 2047
CviQI GTAC 5 cut(s) 1013, 1029, 1897, 1983, 2047
DdeI CTNAG 7 cut(s) 504, 783, 1227, 1254, 1379, 2061, 2658
DraIII CACNNNGTG 2 cut(s) 2384, 2560
Eam1104I CTCTTC 6 cut(s) 600, 756, 1056, 1298, 1578, 2441
EarI CTCTTC 6 cut(s) 600, 756, 1056, 1298, 1578, 2441
EciI GGCGGA 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 2636
Eco105I TACGTA 1 cut(s) 1868
Eco130I CCWWGG 3 cut(s) 771, 1827, 2574
Eco24I GRGCYC 2 cut(s) 2638, 2650
Eco47I GGWCC 2 cut(s) 97, 992
Eco53kI GAGCTC 1 cut(s) 2636
Eco57I CTGAAG 3 cut(s) 654, 747, 1044
Eco81I CCTNAGG 1 cut(s) 2658
EcoICRI GAGCTC 1 cut(s) 2636
EcoRII CCWGG 4 cut(s) 51, 92, 265, 1181
EcoT14I CCWWGG 3 cut(s) 771, 1827, 2574
EcoT22I ATGCAT 1 cut(s) 310
EcoT38I GRGCYC 2 cut(s) 2638, 2650
ErhI CCWWGG 3 cut(s) 771, 1827, 2574
Esp3I CGTCTC 1 cut(s) 2673
FaqI GGGAC 2 cut(s) 110, 2044
FbaI TGATCA 3 cut(s) 544, 1536, 2449
FblI GTMKAC 1 cut(s) 2360
Fnu4HI GCNGC 6 cut(s) 204, 317, 849, 1800, 1914, 2139
FokI GGATG 5 cut(s) 806, 857, 927, 1202, 2650
FriOI GRGCYC 2 cut(s) 2638, 2650
Fsp4HI GCNGC 6 cut(s) 204, 317, 849, 1800, 1914, 2139
FspBI CTAG 5 cut(s) 1095, 1413, 1449, 1461, 1557
GlaI GCGC 1 cut(s) 2707
GluI GCNGC 6 cut(s) 204, 317, 849, 1800, 1914, 2139
GsaI CCCAGC 2 cut(s) 2182, 2311
GsuI CTGGAG 4 cut(s) 697, 710, 2493, 2568
HapII CCGG 2 cut(s) 440, 2667
HhaI GCGC 1 cut(s) 2708
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 2706
HinP1I GCGC 1 cut(s) 2706
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 4 cut(s) 434, 1376, 2252, 2714
HpaII CCGG 2 cut(s) 440, 2667
Hpy166II GTNNAC 5 cut(s) 280, 920, 965, 1899, 2361
Hpy8I GTNNAC 5 cut(s) 280, 920, 965, 1899, 2361
Hpy99I CGWCG 2 cut(s) 10, 1890
HpyAV CCTTC 9 cut(s) 4, 30, 1019, 1096, 1449, 1474, 1811, 1867, 2470
HpyCH4III ACNGT 6 cut(s) 1033, 1487, 1772, 2051, 2417, 2618
HpyCH4IV ACGT 1 cut(s) 1867
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 351, 1306, 1692, 1796
HpyF3I CTNAG 7 cut(s) 504, 783, 1227, 1254, 1379, 2061, 2658
HpySE526I ACGT 1 cut(s) 1867
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 2706
Kpn2I TCCGGA 1 cut(s) 439
Ksp22I TGATCA 3 cut(s) 544, 1536, 2449
KspI CCGCGG 1 cut(s) 157
LguI GCTCTTC 1 cut(s) 1578
LmnI GCTCC 4 cut(s) 43, 342, 958, 2641
Lsp1109I GCAGC 6 cut(s) 190, 303, 860, 1786, 1900, 2125
LweI GCATC 6 cut(s) 295, 835, 875, 1786, 2455, 2672
MaeI CTAG 5 cut(s) 1095, 1413, 1449, 1461, 1557
MaeII ACGT 1 cut(s) 1867
MaeIII GTNAC 2 cut(s) 208, 1487
MfeI CAATTG 1 cut(s) 594
MflI RGATCY 7 cut(s) 55, 838, 925, 1079, 1382, 2291, 2512
MhlI GDGCHC 2 cut(s) 2638, 2650
MlyI GAGTC 3 cut(s) 701, 776, 970
Mph1103I ATGCAT 1 cut(s) 310
MroI TCCGGA 1 cut(s) 439
MroXI GAANNNNTTC 2 cut(s) 1746, 2587
MseI TTAA 8 cut(s) 260, 312, 447, 1526, 1700, 1718, 2321, 2722
MslI CAYNNNNRTG 1 cut(s) 1593
MspA1I CMGCKG 2 cut(s) 156, 1695
MspI CCGG 2 cut(s) 440, 2667
MspR9I CCNGG 5 cut(s) 53, 94, 267, 1183, 2668
MunI CAATTG 1 cut(s) 594
Mva1269I GAATGC 2 cut(s) 421, 1622
MvaI CCWGG 4 cut(s) 53, 94, 267, 1183
MvnI CGCG 2 cut(s) 156, 2706
MwoI GCNNNNNNNGC 5 cut(s) 11, 351, 1306, 1692, 1796
NciI CCSGG 1 cut(s) 2668
NcoI CCATGG 1 cut(s) 771
NlaIV GGNNCC 6 cut(s) 45, 57, 98, 376, 1249, 2211
NmeAIII GCCGAG 1 cut(s) 967
NmuCI GTSAC 1 cut(s) 208
NsiI ATGCAT 1 cut(s) 310
NspI RCATGY 2 cut(s) 1592, 2037
PaqCI CACCTGC 2 cut(s) 1558, 1781
PciI ACATGT 1 cut(s) 2033
PciSI GCTCTTC 1 cut(s) 1578
PctI GAATGC 2 cut(s) 421, 1622
PdmI GAANNNNTTC 2 cut(s) 1746, 2587
PfeI GAWTC 9 cut(s) 125, 395, 629, 857, 1166, 1331, 1337, 1432, 1469
PkrI GCNGC 6 cut(s) 205, 318, 850, 1801, 1915, 2140
PleI GAGTC 3 cut(s) 700, 775, 969
PpsI GAGTC 3 cut(s) 700, 775, 969
Ppu21I YACGTR 1 cut(s) 1868
PscI ACATGT 1 cut(s) 2033
PsiI TTATAA 2 cut(s) 225, 755
Psp124BI GAGCTC 1 cut(s) 2638
Psp6I CCWGG 4 cut(s) 51, 92, 265, 1181
PspFI CCCAGC 2 cut(s) 2178, 2307
PspGI CCWGG 4 cut(s) 51, 92, 265, 1181
PspN4I GGNNCC 6 cut(s) 45, 57, 98, 376, 1249, 2211
PspPI GGNCC 2 cut(s) 97, 992
PstI CTGCAG 2 cut(s) 135, 2143
PsuI RGATCY 7 cut(s) 55, 838, 925, 1079, 1382, 2291, 2512
PvuII CAGCTG 1 cut(s) 1695
RsaI GTAC 5 cut(s) 1014, 1030, 1898, 1984, 2048
RsaNI GTAC 5 cut(s) 1013, 1029, 1897, 1983, 2047
RseI CAYNNNNRTG 1 cut(s) 1593
SacI GAGCTC 1 cut(s) 2638
SacII CCGCGG 1 cut(s) 157
SapI GCTCTTC 1 cut(s) 1578
SaqAI TTAA 8 cut(s) 260, 312, 447, 1526, 1700, 1718, 2321, 2722
SatI GCNGC 6 cut(s) 204, 317, 849, 1800, 1914, 2139
Sau96I GGNCC 2 cut(s) 97, 992
ScaI AGTACT 1 cut(s) 2048
SchI GAGTC 3 cut(s) 701, 776, 970
ScrFI CCNGG 5 cut(s) 53, 94, 267, 1183, 2668
SduI GDGCHC 2 cut(s) 2638, 2650
SfaNI GCATC 6 cut(s) 295, 835, 875, 1786, 2455, 2672
SfcI CTRYAG 2 cut(s) 131, 2139
Sfr303I CCGCGG 1 cut(s) 157
SgrBI CCGCGG 1 cut(s) 157
SinI GGWCC 2 cut(s) 97, 992
SmiMI CAYNNNNRTG 1 cut(s) 1593
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
SnaBI TACGTA 1 cut(s) 1868
SpeI ACTAGT 3 cut(s) 1412, 1448, 1556
SsiI CCGC 3 cut(s) 24, 154, 156
SspMI CTAG 5 cut(s) 1095, 1413, 1449, 1461, 1557
SstI GAGCTC 1 cut(s) 2638
StyD4I CCNGG 5 cut(s) 51, 92, 265, 1181, 2666
StyI CCWWGG 3 cut(s) 771, 1827, 2574
TaaI ACNGT 6 cut(s) 1033, 1487, 1772, 2051, 2417, 2618
TaiI ACGT 1 cut(s) 1870
TaqI TCGA 5 cut(s) 8, 515, 526, 1662, 1925
TatI WGTACW 3 cut(s) 1896, 1982, 2046
TfiI GAWTC 9 cut(s) 125, 395, 629, 857, 1166, 1331, 1337, 1432, 1469
Tru1I TTAA 8 cut(s) 260, 312, 447, 1526, 1700, 1718, 2321, 2722
Tru9I TTAA 8 cut(s) 260, 312, 447, 1526, 1700, 1718, 2321, 2722
TscAI CASTG 4 cut(s) 1168, 1546, 1627, 2015
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 6 cut(s) 203, 316, 848, 1799, 1913, 2138
Tsp45I GTSAC 1 cut(s) 208
TspGWI ACGGA 2 cut(s) 806, 1030
TspRI CASTG 4 cut(s) 1168, 1546, 1627, 2015
VpaK11BI GGWCC 2 cut(s) 97, 992
XapI RAATTY 4 cut(s) 534, 979, 1859, 2422
XbaI TCTAGA 1 cut(s) 1094
XceI RCATGY 2 cut(s) 1592, 2037
XcmI CCANNNNNNNNNTGG 1 cut(s) 1997
XmiI GTMKAC 1 cut(s) 2360
XmnI GAANNNNTTC 2 cut(s) 1746, 2587
XspI CTAG 5 cut(s) 1095, 1413, 1449, 1461, 1557
ZrmI AGTACT 1 cut(s) 2048
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.