MD02G1175000.v1.1

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009363
Physical Location & Seq
Reverse (-)
15383030 .. 15389801
6772 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1175000.v1.1.491

Sequence Viewer

Length: 2352 bp
ATGGCGTCGAAGGCTTCGCCTTCAGCTTCCGCCAATAGATCGGAGCTTCACGAGGACCCTCCGATGACGAGATCGCAAGAAAACATGGATGTAACCCGGAAGGAGATTTCACTGCTCATAGAAAATCTAAAGAAGCAAGTTGTTTTTAACCGGGCTACTTCTATAAAGAAAAGAATGGAGGTAAACAAGCAGAAGTTGGCTGGTGTCACCAACGACATGTATAAATTGTCAACGGAAAGAGGAAGTAGTAGGTTAACTGATGCTAGTAGAAGTGTAGATCTATTGACAAAGAGGCAAAAAGATGCATTTGCCTTGCAAAATGGAATTGAATTAAGCAATGAGAGCAACGATAGCTCTCAAGAAGATGGTCATGGGTCCACGGCAGTTCTACTAGAATCTAATGTAGCAGTCAAGAGTTCTGTTCGTCCTATCAAGCTTCCGGAAGTTAAAAGACTACCCCCTTATACCGCATGGATATTTTTGGACAGAAACCAAAGAATGACAGAAGATCAGTCAGTGGTAGGTCGAAGAAGAATTTATTATGATCAAAATGGAGGTGAAGCGCTTATATGTAGTGATAGTGAGGAGGAAGCAGTTGATGAAGAAGAGGAAAAAAGGGTTTTTGTTGAATCTGAAGACTTTATACTTCGCTCGGCTATAAAAGAAGTTGGCATCTCTGATCCAGTGCTGGAGTCATTAGCACAATATTTCTCTAGAAGTCCATCTGAAGTCAAGGCAAGATATGATACTCTCTTAAAGGAAGAGGAGGCTTCGGGGGGATGTAAGAATAGGGATACTGAAGACAGTTCACAAATTGGGAATTCTTTTCTTGATAAAGATCTGGATGCAGCTCTCGATTCATTTGACAACCTATTTTGTCGTCGCTGCCTAGTATTTGATTGCAGATTACATGGATGTTCACAGGATCTTGTCTTTCCTATTGAGAAACAACCTCTTTGGAGACCCCCAGATGATGAAAAAGTATCATGCGGTCCGAATTGCTATAGATCAGTACTGAAGTCTGAAAGAATTGCTAGAGTGAGCAATGGTGATCCTGAAGAAAAAAATGCCACGTCATTGGATGGTGCTAGTGCTCAAATATCAACCAGAAAGAAATCTTCTAGAAAGAAGTCAAAGTCTGGCCAAAGTGAAAGTGCTTCATCAAATGCAAAAAACATCTCAGAAAGCAGTGATTCAGAGAATGGATCCAGGCAGGGTAGTGATAAAAATCATCAATCGCCCACAAAGACTAGGCTAGCTGGAAAAAGTGGAATTCGTAAGAGGAACAGCAAGAGAGTTGCAGAACGTGTTTTAGTTTGCATGCAGAAGAGGCAGAAGAAAATGGTGGTTTCTGATTCCGATTCCATTGTTAATGTTGGTCTTTGTTCAAGTGATATGAAGTTTAGATCTAATTCATCCAAAGATAATGAAGACACTAGTTCTTCTTCACAAAAGAATCTGAAATCTTCTACCAGTGGAGGGTTTAGGAGGAAGGAATCACCTGCCAAGGGCAGTAATACAGTTGTTCAGGGTGAATCTCTTGATGGTGCATTGGATGAGATGATCGCTGATCCACCTGCCACAAGTAGTGATGACAATTTGAGGAGAGAAGAATGTGTTGATGAAAACATATATAAACAAGAATTAAGTGATGATAAAACATGGAAAACCATTGAGAAGGGCCTTTTTGAGAAAGGCAGAGAGATATTTGGCAGCAACAGCTGCTTAATAGCAAGGAACCTTTTAAATGGTATGAAAACGTGTTGGGAGGTGTTTCAGTATATGAATTTCTCAGAGAGTAAGATGTCCTGCCAAGCAGGTGATGCTGCAAACTCCCTTGTTGAAGGGTATTCCAAGGGTAATAATGAAGCTAGAAGAAGATCAAAATTTTTACGTAGAAGGGGTAGAGTTCGTCGTTTGAAGTATACTTGGAAATCTGCTGCTTACCATTCAATCAGGAAACGAATTACTGAGAGAAAAGATCAGCCTTGCAGGCAGTATAATCCATGTACTTGCCAAACAGCTTGTGGAAAACAGTGTTCTTGTCTACAAAATGGGACTTGTTGTGAGAAGTACTGTGGGTGTCCAAAGACTTGCAAAAATAGATTTAGAGGCTGTCATTGTGCTAAAAGTCAATGCCGAAGCCGTCAGTGTCCTTGTTTTGCTGCAGACAGGGAATGCGATCCGGATGTTTGTAGGAATTGCTGGGTCAGTTGCGGTGACGGTACCCTTGGGGTTCCAAGCCAAAGAGGTGACAATTATGAGTGTAGAAATATGAAGCTTCTTCTGAAACAACAGCAAAGGGTAATCACACCTCTTTACTGTTTCATTTCTTTTTCTTTCCTTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003700 GO:0003723 GO:0003727 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005677 GO:0006139 GO:0006325 GO:0006342 GO:0006349 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009292 GO:0009294 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009965 GO:0009987 GO:0010016 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016278 GO:0016279 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0017053 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031519 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0040030 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044764 GO:0045814 GO:0045857 GO:0045892 GO:0045934 GO:0046483 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048586 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051704 GO:0060255 GO:0061458 GO:0065007 GO:0065009 GO:0070013 GO:0070734 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090568 GO:0097159 GO:0099402 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1905392 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00310 ko05206 map00310 map05206
Pfam Domains
Protein Families
SET

Protein Analysis

784

Amino Acids

87.88

Weight (kDa)

8.84

Isoelectric Point (pI)

65.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_CLF_N PF25996 208 - 251 2.2e-19 Histone-lysine N-methyltransferase CLF, N-terminal HTH domain
preSET_CXC PF18264 705 - 736 7.3e-08 CXC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1510, 1585, 1808
Acc36I ACCTGC 3 cut(s) 1510, 1585, 1808
Acc65I GGTACC 1 cut(s) 2225
AccB1I GGYRCC 1 cut(s) 2225
AccI GTMKAC 2 cut(s) 1925, 2047
AccIII TCCGGA 2 cut(s) 439, 2185
AciI CCGC 4 cut(s) 30, 468, 990, 2217
AclWI GGATC 7 cut(s) 674, 933, 1046, 1200, 1213, 1565, 2177
AcoI YGGCCR 1 cut(s) 1141
AcsI RAATTY 5 cut(s) 534, 820, 1272, 1786, 1886
AcuI CTGAAG 6 cut(s) 6, 654, 747, 819, 1037, 1077
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 1014, 2011, 2075, 2227
AfeI AGCGCT 1 cut(s) 564
AfiI CCNNNNNNNGG 2 cut(s) 1479, 1508
AflIII ACRYGT 3 cut(s) 216, 1306, 1760
AgsI TTSAA 6 cut(s) 329, 629, 1389, 1844, 1921, 1953
AhlI ACTAGT 1 cut(s) 1436
AjiI CACGTC 1 cut(s) 1074
AjnI CCWGG 1 cut(s) 1208
Alw21I GWGCWC 1 cut(s) 1096
Alw26I GTCTC 1 cut(s) 955
AlwI GGATC 7 cut(s) 674, 933, 1046, 1200, 1213, 1565, 2177
Aor13HI TCCGGA 2 cut(s) 439, 2185
Aor51HI AGCGCT 1 cut(s) 564
AoxI GGCC 2 cut(s) 1141, 1681
ApeKI GCWGC 7 cut(s) 848, 885, 1713, 1722, 1826, 1940, 2165
ApoI RAATTY 5 cut(s) 534, 820, 1272, 1786, 1886
Asp718I GGTACC 1 cut(s) 2225
AspLEI GCGC 1 cut(s) 565
AspS9I GGNCC 4 cut(s) 55, 375, 992, 1681
AsuC2I CCSGG 2 cut(s) 97, 152
AsuHPI GGTGA 8 cut(s) 199, 569, 1061, 1491, 1544, 1832, 2231, 2264
AsuNHI GCTAGC 1 cut(s) 1255
AvaII GGWCC 3 cut(s) 55, 375, 992
BalI TGGCCA 1 cut(s) 1143
BamHI GGATCC 1 cut(s) 1205
BanI GGYRCC 1 cut(s) 2225
BarI GAAGNNNNNNTAC 2 cut(s) 627, 659
BauI CACGAG 1 cut(s) 50
BbsI GAAGAC 3 cut(s) 642, 807, 1437
Bbv12I GWGCWC 1 cut(s) 1096
BbvI GCAGC 7 cut(s) 860, 872, 1709, 1725, 1813, 1927, 2152
BccI CCATC 4 cut(s) 359, 730, 1076, 1538
BceAI ACGGC 2 cut(s) 396, 2130
BciT130I CCWGG 1 cut(s) 1210
BciVI GTATCC 1 cut(s) 787
BclI TGATCA 1 cut(s) 544
BcnI CCSGG 2 cut(s) 97, 152
BcoDI GTCTC 1 cut(s) 955
BcuI ACTAGT 1 cut(s) 1436
BfmI CTRYAG 2 cut(s) 1003, 2166
BfoI RGCGCY 1 cut(s) 566
BfuAI ACCTGC 3 cut(s) 1510, 1585, 1808
BfuI GTATCC 1 cut(s) 787
BglI GCCNNNNNGGC 1 cut(s) 1993
BglII AGATCT 3 cut(s) 277, 838, 1406
BisI GCNGC 7 cut(s) 849, 886, 1714, 1723, 1827, 1941, 2166
BlsI GCNGC 7 cut(s) 850, 887, 1715, 1724, 1828, 1942, 2167
BmcAI AGTACT 2 cut(s) 1014, 2075
Bme1390I CCNGG 3 cut(s) 97, 152, 1210
Bme18I GGWCC 3 cut(s) 55, 375, 992
BmgBI CACGTC 1 cut(s) 1074
BmgT120I GGNCC 4 cut(s) 55, 375, 992, 1681
BmiI GGNNCC 6 cut(s) 57, 376, 1207, 1739, 2227, 2238
BmrFI CCNGG 3 cut(s) 97, 152, 1210
BmsI GCATC 5 cut(s) 250, 292, 681, 835, 1813
BmtI GCTAGC 1 cut(s) 1259
BpiI GAAGAC 3 cut(s) 642, 807, 1437
BpmI CTGGAG 1 cut(s) 710
BpuEI CTTGAG 1 cut(s) 342
BpuMI CCSGG 2 cut(s) 97, 152
BsaAI YACGTR 1 cut(s) 1895
BsaBI GATNNNNATC 2 cut(s) 837, 1227
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 955
BsaJI CCNNGG 4 cut(s) 378, 1506, 1854, 2230
BsaWI WCCGGW 2 cut(s) 439, 2185
Bsc4I CCNNNNNNNGG 2 cut(s) 1479, 1508
Bse1I ACTGG 2 cut(s) 683, 1473
Bse3DI GCAATG 2 cut(s) 343, 1051
Bse8I GATNNNNATC 2 cut(s) 837, 1227
BseAI TCCGGA 2 cut(s) 439, 2185
BseBI CCWGG 1 cut(s) 1210
BseDI CCNNGG 4 cut(s) 378, 1506, 1854, 2230
BseGI GGATG 8 cut(s) 94, 785, 850, 920, 1087, 1415, 1561, 2194
BseJI GATNNNNATC 2 cut(s) 837, 1227
BseLI CCNNNNNNNGG 2 cut(s) 1479, 1508
BseMI GCAATG 2 cut(s) 343, 1051
BseMII CTCAG 3 cut(s) 1194, 1806, 1962
BseNI ACTGG 2 cut(s) 683, 1473
BseRI GAGGAG 3 cut(s) 599, 779, 1618
BseXI GCAGC 7 cut(s) 860, 872, 1709, 1725, 1813, 1927, 2152
BseYI CCCAGC 1 cut(s) 2205
BshFI GGCC 2 cut(s) 1143, 1683
BshNI GGYRCC 1 cut(s) 2225
BsiHKAI GWGCWC 1 cut(s) 1096
BsiSI CCGG 4 cut(s) 97, 151, 440, 2186
BslFI GGGAC 1 cut(s) 2071
BslI CCNNNNNNNGG 2 cut(s) 1479, 1508
BsmAI GTCTC 1 cut(s) 955
BsmFI GGGAC 1 cut(s) 2071
BsmI GAATGC 1 cut(s) 2183
BsnI GGCC 2 cut(s) 1143, 1683
Bso31I GGTCTC 1 cut(s) 955
Bsp1286I GDGCHC 1 cut(s) 1096
Bsp13I TCCGGA 2 cut(s) 439, 2185
BspACI CCGC 4 cut(s) 30, 468, 990, 2217
BspANI GGCC 2 cut(s) 1143, 1683
BspCNI CTCAG 3 cut(s) 1193, 1805, 1963
BspEI TCCGGA 2 cut(s) 439, 2185
BspLI GGNNCC 6 cut(s) 57, 376, 1207, 1739, 2227, 2238
BspMAI CTGCAG 1 cut(s) 2170
BspMI ACCTGC 3 cut(s) 1510, 1585, 1808
BspOI GCTAGC 1 cut(s) 1259
BspPI GGATC 7 cut(s) 674, 933, 1046, 1200, 1213, 1565, 2177
BspT107I GGYRCC 1 cut(s) 2225
BspTNI GGTCTC 1 cut(s) 955
BsrDI GCAATG 2 cut(s) 343, 1051
BsrI ACTGG 2 cut(s) 683, 1473
BssECI CCNNGG 4 cut(s) 378, 1506, 1854, 2230
BssNAI GTATAC 1 cut(s) 1926
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 50
BssT1I CCWWGG 3 cut(s) 1506, 1854, 2230
Bst1107I GTATAC 1 cut(s) 1926
Bst2BI CACGAG 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 1210
Bst4CI ACNGT 6 cut(s) 806, 1522, 2037, 2078, 2225, 2324
Bst6I CTCTTC 3 cut(s) 600, 756, 1322
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 2 cut(s) 1722, 1823
BstBAI YACGTR 1 cut(s) 1895
BstC8I GCNNGC 3 cut(s) 1257, 1322, 1994
BstDEI CTNAG 3 cut(s) 1180, 1792, 1971
BstDSI CCRYGG 1 cut(s) 378
BstF5I GGATG 8 cut(s) 94, 785, 850, 920, 1087, 1415, 1561, 2194
BstH2I RGCGCY 1 cut(s) 566
BstHHI GCGC 1 cut(s) 565
BstMAI GTCTC 1 cut(s) 955
BstMWI GCNNNNNNNGC 8 cut(s) 11, 342, 351, 1330, 1719, 1722, 1823, 1993
BstNI CCWGG 1 cut(s) 1210
BstNSI RCATGY 2 cut(s) 220, 1324
BstSCI CCNGG 3 cut(s) 95, 150, 1208
BstSFI CTRYAG 2 cut(s) 1003, 2166
BstSNI TACGTA 1 cut(s) 1895
BstV1I GCAGC 7 cut(s) 860, 872, 1709, 1725, 1813, 1927, 2152
BstV2I GAAGAC 3 cut(s) 642, 807, 1437
BstX2I RGATCY 5 cut(s) 277, 838, 925, 1205, 1406
BstXI CCANNNNNNTGG 1 cut(s) 1078
BstYI RGATCY 5 cut(s) 277, 838, 925, 1205, 1406
BstZ17I GTATAC 1 cut(s) 1926
BsuI GTATCC 1 cut(s) 787
BsuRI GGCC 2 cut(s) 1143, 1683
BtgI CCRYGG 1 cut(s) 378
BtrI CACGTC 1 cut(s) 1074
BtsCI GGATG 8 cut(s) 94, 785, 850, 920, 1087, 1415, 1561, 2194
BtsI GCAGTG 2 cut(s) 110, 1195
BtsIMutI CAGTG 7 cut(s) 110, 522, 690, 1195, 1480, 2042, 2156
BveI ACCTGC 3 cut(s) 1510, 1585, 1808
Cac8I GCNNGC 3 cut(s) 1257, 1322, 1994
CfoI GCGC 1 cut(s) 565
Cfr13I GGNCC 4 cut(s) 55, 375, 992, 1681
CpoI CGGWCCG 1 cut(s) 992
Csp6I GTAC 4 cut(s) 1013, 2010, 2074, 2226
CspI CGGWCCG 1 cut(s) 992
CviAII CATG 9 cut(s) 85, 217, 371, 471, 911, 987, 1321, 1662, 2007
CviQI GTAC 4 cut(s) 1013, 2010, 2074, 2226
DdeI CTNAG 3 cut(s) 1180, 1792, 1971
DraI TTTAAA 1 cut(s) 1746
EaeI YGGCCR 1 cut(s) 1141
Eam1104I CTCTTC 3 cut(s) 600, 756, 1322
EarI CTCTTC 3 cut(s) 600, 756, 1322
EciI GGCGGA 1 cut(s) 19
Eco105I TACGTA 1 cut(s) 1895
Eco130I CCWWGG 3 cut(s) 1506, 1854, 2230
Eco31I GGTCTC 1 cut(s) 955
Eco47I GGWCC 3 cut(s) 55, 375, 992
Eco47III AGCGCT 1 cut(s) 564
Eco57I CTGAAG 6 cut(s) 6, 654, 747, 819, 1037, 1077
EcoO109I RGGNCCY 2 cut(s) 55, 1681
EcoRI GAATTC 2 cut(s) 820, 1272
EcoRII CCWGG 1 cut(s) 1208
EcoT14I CCWWGG 3 cut(s) 1506, 1854, 2230
EcoT22I ATGCAT 1 cut(s) 307
ErhI CCWWGG 3 cut(s) 1506, 1854, 2230
FaeI CATG 9 cut(s) 88, 220, 374, 474, 914, 990, 1324, 1665, 2010
FaqI GGGAC 1 cut(s) 2071
FatI CATG 9 cut(s) 84, 216, 370, 470, 910, 986, 1320, 1661, 2006
FbaI TGATCA 1 cut(s) 544
FblI GTMKAC 2 cut(s) 1925, 2047
Fnu4HI GCNGC 7 cut(s) 849, 886, 1714, 1723, 1827, 1941, 2166
FokI GGATG 8 cut(s) 101, 792, 857, 927, 1094, 1402, 1568, 2201
Fsp4HI GCNGC 7 cut(s) 849, 886, 1714, 1723, 1827, 1941, 2166
GlaI GCGC 1 cut(s) 564
GluI GCNGC 7 cut(s) 849, 886, 1714, 1723, 1827, 1941, 2166
GsaI CCCAGC 1 cut(s) 2209
GsuI CTGGAG 1 cut(s) 710
HaeII RGCGCY 1 cut(s) 566
HaeIII GGCC 2 cut(s) 1143, 1683
HapII CCGG 4 cut(s) 97, 151, 440, 2186
HhaI GCGC 1 cut(s) 565
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 9 cut(s) 88, 220, 374, 474, 914, 990, 1324, 1665, 2010
Hin6I GCGC 1 cut(s) 563
HinP1I GCGC 1 cut(s) 563
HincII GTYRAC 2 cut(s) 231, 255
HindII GTYRAC 2 cut(s) 231, 255
HindIII AAGCTT 2 cut(s) 434, 2279
HpaI GTTAAC 1 cut(s) 255
HpaII CCGG 4 cut(s) 97, 151, 440, 2186
HphI GGTGA 8 cut(s) 199, 569, 1061, 1491, 1544, 1832, 2231, 2264
Hpy166II GTNNAC 8 cut(s) 184, 231, 255, 378, 809, 920, 1926, 2048
Hpy8I GTNNAC 8 cut(s) 184, 231, 255, 378, 809, 920, 1926, 2048
Hpy99I CGWCG 3 cut(s) 10, 885, 1917
HpyAV CCTTC 7 cut(s) 4, 30, 94, 1486, 1672, 1838, 1893
HpyCH4III ACNGT 6 cut(s) 806, 1522, 2037, 2078, 2225, 2324
HpyCH4IV ACGT 4 cut(s) 1073, 1306, 1760, 1894
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 342, 351, 1330, 1719, 1722, 1823, 1993
HpyF3I CTNAG 3 cut(s) 1180, 1792, 1971
HpySE526I ACGT 4 cut(s) 1073, 1306, 1760, 1894
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 9 cut(s) 88, 220, 374, 474, 914, 990, 1324, 1665, 2010
HspAI GCGC 1 cut(s) 563
Kpn2I TCCGGA 2 cut(s) 439, 2185
KpnI GGTACC 1 cut(s) 2229
Ksp22I TGATCA 1 cut(s) 544
KspAI GTTAAC 1 cut(s) 255
LmnI GCTCC 1 cut(s) 43
Lsp1109I GCAGC 7 cut(s) 860, 872, 1709, 1725, 1813, 1927, 2152
LweI GCATC 5 cut(s) 250, 292, 681, 835, 1813
MaeII ACGT 4 cut(s) 1073, 1306, 1760, 1894
MaeIII GTNAC 4 cut(s) 91, 205, 2219, 2252
MflI RGATCY 5 cut(s) 277, 838, 925, 1205, 1406
MhlI GDGCHC 1 cut(s) 1096
MlsI TGGCCA 1 cut(s) 1143
MluNI TGGCCA 1 cut(s) 1143
MlyI GAGTC 1 cut(s) 701
Mox20I TGGCCA 1 cut(s) 1143
Mph1103I ATGCAT 1 cut(s) 307
MroI TCCGGA 2 cut(s) 439, 2185
MscI TGGCCA 1 cut(s) 1143
MseI TTAA 9 cut(s) 147, 254, 332, 447, 755, 1371, 1646, 1727, 1745
Msp20I TGGCCA 1 cut(s) 1143
MspA1I CMGCKG 1 cut(s) 1722
MspI CCGG 4 cut(s) 97, 151, 440, 2186
MspR9I CCNGG 3 cut(s) 97, 152, 1210
Mva1269I GAATGC 1 cut(s) 2183
MvaI CCWGG 1 cut(s) 1210
MwoI GCNNNNNNNGC 8 cut(s) 11, 342, 351, 1330, 1719, 1722, 1823, 1993
NciI CCSGG 2 cut(s) 97, 152
NheI GCTAGC 1 cut(s) 1255
NlaIII CATG 9 cut(s) 88, 220, 374, 474, 914, 990, 1324, 1665, 2010
NlaIV GGNNCC 6 cut(s) 57, 376, 1207, 1739, 2227, 2238
NmeAIII GCCGAG 1 cut(s) 632
NmuCI GTSAC 3 cut(s) 205, 2219, 2252
NsiI ATGCAT 1 cut(s) 307
NspI RCATGY 2 cut(s) 220, 1324
PaeI GCATGC 1 cut(s) 1324
PaqCI CACCTGC 3 cut(s) 1510, 1585, 1808
PciI ACATGT 1 cut(s) 216
PctI GAATGC 1 cut(s) 2183
PfeI GAWTC 9 cut(s) 395, 629, 857, 1193, 1355, 1361, 1456, 1496, 1535
PkrI GCNGC 7 cut(s) 850, 887, 1715, 1724, 1828, 1942, 2167
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
Ppu21I YACGTR 1 cut(s) 1895
PpuMI RGGWCCY 1 cut(s) 55
PscI ACATGT 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 1208
PspFI CCCAGC 1 cut(s) 2205
PspGI CCWGG 1 cut(s) 1208
PspN4I GGNNCC 6 cut(s) 57, 376, 1207, 1739, 2227, 2238
PspPI GGNCC 4 cut(s) 55, 375, 992, 1681
PspPPI RGGWCCY 1 cut(s) 55
PstI CTGCAG 1 cut(s) 2170
PsuI RGATCY 5 cut(s) 277, 838, 925, 1205, 1406
PvuII CAGCTG 1 cut(s) 1722
RsaI GTAC 4 cut(s) 1014, 2011, 2075, 2227
RsaNI GTAC 4 cut(s) 1013, 2010, 2074, 2226
Rsr2I CGGWCCG 1 cut(s) 992
RsrII CGGWCCG 1 cut(s) 992
SaqAI TTAA 9 cut(s) 147, 254, 332, 447, 755, 1371, 1646, 1727, 1745
SatI GCNGC 7 cut(s) 849, 886, 1714, 1723, 1827, 1941, 2166
Sau96I GGNCC 4 cut(s) 55, 375, 992, 1681
ScaI AGTACT 2 cut(s) 1014, 2075
SchI GAGTC 1 cut(s) 701
ScrFI CCNGG 3 cut(s) 97, 152, 1210
SduI GDGCHC 1 cut(s) 1096
SfaNI GCATC 5 cut(s) 250, 292, 681, 835, 1813
SfcI CTRYAG 2 cut(s) 1003, 2166
SinI GGWCC 3 cut(s) 55, 375, 992
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
SnaBI TACGTA 1 cut(s) 1895
SpeI ACTAGT 1 cut(s) 1436
SphI GCATGC 1 cut(s) 1324
SsiI CCGC 4 cut(s) 30, 468, 990, 2217
SspI AATATT 1 cut(s) 707
StyD4I CCNGG 3 cut(s) 95, 150, 1208
StyI CCWWGG 3 cut(s) 1506, 1854, 2230
TaaI ACNGT 6 cut(s) 806, 1522, 2037, 2078, 2225, 2324
TaiI ACGT 4 cut(s) 1076, 1309, 1763, 1897
TaqI TCGA 3 cut(s) 8, 526, 855
TatI WGTACW 3 cut(s) 1012, 2009, 2073
TfiI GAWTC 9 cut(s) 395, 629, 857, 1193, 1355, 1361, 1456, 1496, 1535
Tru1I TTAA 9 cut(s) 147, 254, 332, 447, 755, 1371, 1646, 1727, 1745
Tru9I TTAA 9 cut(s) 147, 254, 332, 447, 755, 1371, 1646, 1727, 1745
TscAI CASTG 7 cut(s) 117, 522, 690, 1195, 1480, 2042, 2156
TseFI GTSAC 3 cut(s) 205, 2219, 2252
TseI GCWGC 7 cut(s) 848, 885, 1713, 1722, 1826, 1940, 2165
Tsp45I GTSAC 3 cut(s) 205, 2219, 2252
TspGWI ACGGA 1 cut(s) 248
TspRI CASTG 7 cut(s) 117, 522, 690, 1195, 1480, 2042, 2156
VpaK11BI GGWCC 3 cut(s) 55, 375, 992
XapI RAATTY 5 cut(s) 534, 820, 1272, 1786, 1886
XbaI TCTAGA 2 cut(s) 713, 1121
XceI RCATGY 2 cut(s) 220, 1324
XcmI CCANNNNNNNNNTGG 1 cut(s) 2024
XmiI GTMKAC 2 cut(s) 1925, 2047
ZrmI AGTACT 2 cut(s) 1014, 2075
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.