FvH4_1g16800

MRG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010752
Physical Location & Seq
Reverse (-)
9727027 .. 9739754
12728 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16800.t3

Sequence Viewer

Length: 930 bp
ATGGGGAACTCTTCGACCGATGACTCTGCCTCCTTCGACGGCGACGCTCCGCTCTTCTCCGCCGGCGAGAAGGTCCTCGCCTTCCACAACTCTCGCATCTACGAAGCCAAGGTTCAGAAGGCAGAGATCAGGAAAAACGAATGGAGATACTTTGTTCACTACCTTGGCTGGAATAAAAATTGGGACGAGTGGGTAGGTGTGGATCGCATATTGAAGTACACTGAAGAGAATATAATGAAGCAGCAAGCTCTTGACAAGAAACAAGGCGTAGACAAGAATACAAAGTCAGGACGCTCCGCTCACACAAAGCCAAAAAGTTCTGCTGATGCAAAAGCGGAGAAGGAGGACCAAAAGAACAATGTGGCCAAAGGGAAGAAGCGAAAGAATGACTCAGGCACTGAGAAGGAAAGCGTCAACTTGGAAAAGCTTGTGAAGATTCAAATTCCTGTTTCCTTAAGAAAGCAACTGGTGGAGGATTGGGAATTTGTTTGTGAGCAGGATAAGCTTGTTAACCTTCCACGATCTCCAACTGTAGATGACATTTTGCAGAAGTATCTTGACTACAGGACAAAGAAAGATGGAAAGCTGACTGATCCAGTAGGAGAAGTCCTGAAAGGAATACGATGTTACTTTGACAAAGCATTGCCGTTGATTCTCTTATACAAAAAGGAACGCAAACAGTATCATGAAACCGTTGTAGGTGATGTGTCTCCATCAACAATATATGGTGCTGAACACCTGCTACGACTTTTTGTTAAGCTGCCGGAGCTATTGGCGTATGTCAACATTGAGGAGGAAACACTGACCCGCTTACAGCAGCAATTAATTGATTTACTCAAATTTCTGCAGAAGAATCAGAGCACCTTCTTCCTTTCTGCTTATGATGGCTCAAAAAGTGTTGATGGGAAGGGCAAAGGAAAAAACGAGTGA

Protein Analysis

310

Amino Acids

35.45

Weight (kDa)

9.02

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRG PF05712 47 - 216 6.9e-50 MRG
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 747
Acc36I ACCTGC 1 cut(s) 747
AccBSI CCGCTC 2 cut(s) 52, 299
AccI GTMKAC 1 cut(s) 270
AciI CCGC 5 cut(s) 50, 60, 297, 335, 808
AclWI GGATC 2 cut(s) 210, 587
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 3 cut(s) 441, 482, 839
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 1 cut(s) 218
AflII CTTAAG 1 cut(s) 454
AgsI TTSAA 2 cut(s) 214, 440
AluBI AGCT 6 cut(s) 248, 427, 505, 586, 760, 769
AluI AGCT 6 cut(s) 248, 427, 505, 586, 760, 769
Alw21I GWGCWC 1 cut(s) 863
Alw26I GTCTC 1 cut(s) 714
AlwI GGATC 2 cut(s) 210, 587
AlwNI CAGNNNCTG 1 cut(s) 398
AoxI GGCC 1 cut(s) 363
ApeKI GCWGC 3 cut(s) 241, 760, 817
ApoI RAATTY 3 cut(s) 441, 482, 839
AseI ATTAAT 1 cut(s) 824
AspS9I GGNCC 2 cut(s) 73, 346
AsuHPI GGTGA 1 cut(s) 713
AvaII GGWCC 2 cut(s) 73, 346
BalI TGGCCA 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 863
BbvI GCAGC 3 cut(s) 253, 747, 829
BccI CCATC 4 cut(s) 572, 721, 878, 896
BceAI ACGGC 2 cut(s) 55, 631
BcgI CGANNNNNNTGC 2 cut(s) 8, 42
BcoDI GTCTC 1 cut(s) 714
BfmI CTRYAG 3 cut(s) 531, 562, 845
BfrI CTTAAG 1 cut(s) 454
BfuAI ACCTGC 1 cut(s) 747
BisI GCNGC 3 cut(s) 242, 761, 818
BlsI GCNGC 3 cut(s) 243, 762, 819
Bme18I GGWCC 2 cut(s) 73, 346
BmgT120I GGNCC 2 cut(s) 73, 346
BmsI GCATC 2 cut(s) 105, 316
BsaJI CCNNGG 2 cut(s) 108, 163
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 2 cut(s) 471, 596
Bse3DI GCAATG 1 cut(s) 641
BseDI CCNNGG 2 cut(s) 108, 163
BseMI GCAATG 1 cut(s) 641
BseMII CTCAG 2 cut(s) 390, 405
BseNI ACTGG 2 cut(s) 471, 596
BseRI GAGGAG 1 cut(s) 806
BseXI GCAGC 3 cut(s) 253, 747, 829
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 1 cut(s) 365
BsiEI CGRYCG 1 cut(s) 18
BsiHKAI GWGCWC 1 cut(s) 863
BsiSI CCGG 2 cut(s) 63, 764
BslFI GGGAC 1 cut(s) 197
BsmAI GTCTC 1 cut(s) 714
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 863
Bsp143I GATC 4 cut(s) 126, 202, 521, 592
BspACI CCGC 5 cut(s) 50, 60, 297, 335, 808
BspANI GGCC 1 cut(s) 365
BspCNI CTCAG 2 cut(s) 391, 404
BspHI TCATGA 1 cut(s) 685
BspMAI CTGCAG 1 cut(s) 849
BspMI ACCTGC 1 cut(s) 747
BspPI GGATC 2 cut(s) 210, 587
BspQI GCTCTTC 1 cut(s) 59
BspTI CTTAAG 1 cut(s) 454
BsrBI CCGCTC 2 cut(s) 52, 299
BsrDI GCAATG 1 cut(s) 641
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 2 cut(s) 471, 596
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 2 cut(s) 108, 163
BssMI GATC 4 cut(s) 126, 202, 521, 592
BssT1I CCWWGG 2 cut(s) 108, 163
Bst4CI ACNGT 3 cut(s) 532, 681, 694
Bst6I CTCTTC 3 cut(s) 16, 59, 219
BstAFI CTTAAG 1 cut(s) 454
BstC8I GCNNGC 2 cut(s) 64, 246
BstDEI CTNAG 2 cut(s) 391, 399
BstKTI GATC 4 cut(s) 129, 205, 524, 595
BstMAI GTCTC 1 cut(s) 714
BstMBI GATC 4 cut(s) 126, 202, 521, 592
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 2 cut(s) 502, 766
BstSFI CTRYAG 3 cut(s) 531, 562, 845
BstV1I GCAGC 3 cut(s) 253, 747, 829
BsuRI GGCC 1 cut(s) 365
BtsIMutI CAGTG 3 cut(s) 219, 396, 800
BveI ACCTGC 1 cut(s) 747
Cac8I GCNNGC 2 cut(s) 64, 246
CaiI CAGNNNCTG 1 cut(s) 398
CciI TCATGA 1 cut(s) 685
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 2 cut(s) 73, 346
CseI GACGC 3 cut(s) 53, 300, 400
Csp6I GTAC 1 cut(s) 217
CviAII CATG 1 cut(s) 686
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 2 cut(s) 391, 399
DpnI GATC 4 cut(s) 128, 204, 523, 594
DpnII GATC 4 cut(s) 126, 202, 521, 592
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 3 cut(s) 16, 59, 219
EarI CTCTTC 3 cut(s) 16, 59, 219
EciI GGCGGA 1 cut(s) 49
Eco130I CCWWGG 2 cut(s) 108, 163
Eco47I GGWCC 2 cut(s) 73, 346
Eco57I CTGAAG 1 cut(s) 243
EcoO109I RGGNCCY 1 cut(s) 73
EcoT14I CCWWGG 2 cut(s) 108, 163
ErhI CCWWGG 2 cut(s) 108, 163
FaeI CATG 1 cut(s) 689
FaiI YATR 8 cut(s) 209, 233, 661, 687, 724, 726, 780, 882
FaqI GGGAC 1 cut(s) 197
FatI CATG 1 cut(s) 685
FauI CCCGC 1 cut(s) 815
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 3 cut(s) 242, 761, 818
Fsp4HI GCNGC 3 cut(s) 242, 761, 818
GluI GCNGC 3 cut(s) 242, 761, 818
HaeIII GGCC 1 cut(s) 365
HapII CCGG 2 cut(s) 63, 764
HgaI GACGC 3 cut(s) 53, 300, 400
Hin1II CATG 1 cut(s) 689
HincII GTYRAC 3 cut(s) 415, 511, 784
HindII GTYRAC 3 cut(s) 415, 511, 784
HindIII AAGCTT 2 cut(s) 425, 503
HinfI GANTC 5 cut(s) 23, 389, 436, 652, 853
HpaI GTTAAC 1 cut(s) 511
HpaII CCGG 2 cut(s) 63, 764
HphI GGTGA 1 cut(s) 713
Hpy166II GTNNAC 6 cut(s) 157, 219, 271, 415, 511, 784
Hpy188I TCNGA 2 cut(s) 117, 858
Hpy188III TCNNGA 6 cut(s) 130, 251, 288, 557, 610, 686
Hpy8I GTNNAC 6 cut(s) 157, 219, 271, 415, 511, 784
Hpy99I CGWCG 2 cut(s) 41, 47
HpyAV CCTTC 9 cut(s) 43, 64, 91, 112, 334, 397, 524, 874, 901
HpyCH4III ACNGT 3 cut(s) 532, 681, 694
HpyCH4V TGCA 3 cut(s) 329, 547, 847
HpyF10VI GCNNNNNNNGC 2 cut(s) 502, 766
HpyF3I CTNAG 2 cut(s) 391, 399
Hsp92II CATG 1 cut(s) 689
KroI GCCGGC 1 cut(s) 62
KroNI GCCGGC 1 cut(s) 64
KspAI GTTAAC 1 cut(s) 511
Kzo9I GATC 4 cut(s) 126, 202, 521, 592
LguI GCTCTTC 1 cut(s) 59
LmnI GCTCC 3 cut(s) 52, 299, 766
Lsp1109I GCAGC 3 cut(s) 253, 747, 829
LweI GCATC 2 cut(s) 105, 316
MaeIII GTNAC 1 cut(s) 626
MalI GATC 4 cut(s) 128, 204, 523, 594
MbiI CCGCTC 2 cut(s) 52, 299
MboI GATC 4 cut(s) 126, 202, 521, 592
MboII GAAGA 7 cut(s) 3, 46, 236, 385, 445, 859, 862
MhlI GDGCHC 1 cut(s) 863
MlsI TGGCCA 1 cut(s) 365
MluCI AATT 6 cut(s) 178, 441, 482, 821, 825, 839
MluNI TGGCCA 1 cut(s) 365
MlyI GAGTC 2 cut(s) 17, 383
MmeI TCCRAC 1 cut(s) 551
MnlI CCTC 6 cut(s) 40, 86, 337, 466, 784, 787
Mox20I TGGCCA 1 cut(s) 365
MreI CGCCGGCG 1 cut(s) 62
MroNI GCCGGC 1 cut(s) 62
MscI TGGCCA 1 cut(s) 365
MseI TTAA 4 cut(s) 455, 510, 756, 824
Msp20I TGGCCA 1 cut(s) 365
MspCI CTTAAG 1 cut(s) 454
MspI CCGG 2 cut(s) 63, 764
MwoI GCNNNNNNNGC 2 cut(s) 502, 766
NaeI GCCGGC 1 cut(s) 64
NdeII GATC 4 cut(s) 126, 202, 521, 592
NgoMIV GCCGGC 1 cut(s) 62
NlaIII CATG 1 cut(s) 689
PagI TCATGA 1 cut(s) 685
PaqCI CACCTGC 1 cut(s) 747
PciSI GCTCTTC 1 cut(s) 59
PdiI GCCGGC 1 cut(s) 64
PfeI GAWTC 3 cut(s) 436, 652, 853
PkrI GCNGC 3 cut(s) 243, 762, 819
PleI GAGTC 2 cut(s) 17, 383
PpsI GAGTC 2 cut(s) 17, 383
PpuMI RGGWCCY 1 cut(s) 73
PshBI ATTAAT 1 cut(s) 824
Psp5II RGGWCCY 1 cut(s) 73
PspPI GGNCC 2 cut(s) 73, 346
PspPPI RGGWCCY 1 cut(s) 73
PsrI GAACNNNNNNTAC 2 cut(s) 726, 758
PstI CTGCAG 1 cut(s) 849
PstNI CAGNNNCTG 1 cut(s) 398
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
SapI GCTCTTC 1 cut(s) 59
SaqAI TTAA 4 cut(s) 455, 510, 756, 824
SatI GCNGC 3 cut(s) 242, 761, 818
Sau3AI GATC 4 cut(s) 126, 202, 521, 592
Sau96I GGNCC 2 cut(s) 73, 346
SchI GAGTC 2 cut(s) 17, 383
SduI GDGCHC 1 cut(s) 863
SfaNI GCATC 2 cut(s) 105, 316
SfcI CTRYAG 3 cut(s) 531, 562, 845
SgrAI CRCCGGYG 1 cut(s) 62
SinI GGWCC 2 cut(s) 73, 346
SmlI CTYRAG 1 cut(s) 454
SmoI CTYRAG 1 cut(s) 454
Sse9I AATT 6 cut(s) 178, 441, 482, 821, 825, 839
SsiI CCGC 5 cut(s) 50, 60, 297, 335, 808
StyI CCWWGG 2 cut(s) 108, 163
TaaI ACNGT 3 cut(s) 532, 681, 694
TaqI TCGA 2 cut(s) 14, 36
TaqII GACCGA 1 cut(s) 32
TasI AATT 6 cut(s) 178, 441, 482, 821, 825, 839
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 3 cut(s) 436, 652, 853
Tru1I TTAA 4 cut(s) 455, 510, 756, 824
Tru9I TTAA 4 cut(s) 455, 510, 756, 824
TscAI CASTG 3 cut(s) 226, 403, 807
TseI GCWGC 3 cut(s) 241, 760, 817
TspDTI ATGAA 2 cut(s) 251, 702
TspRI CASTG 3 cut(s) 226, 403, 807
Vha464I CTTAAG 1 cut(s) 454
VpaK11BI GGWCC 2 cut(s) 73, 346
VspI ATTAAT 1 cut(s) 824
XapI RAATTY 3 cut(s) 441, 482, 839
XmiI GTMKAC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.