FvH4_1g18310

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0016025
Physical Location & Seq
Reverse (-)
10658277 .. 10660478
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g18310.t1

Sequence Viewer

Length: 2202 bp
ATGCAGTTGAACTACTACAAACAAGTCTTCACGACTATAACCGAAGCCGCCGGTGAAGTACTGAAGCTGAACCAGAGGCTGGTGAAGCTTAACCGCTCGCATTGTTACGTAGAGACGCTTCAGCTCTTCACCAAAATCAATTCATCACAGTCGGTTAGGCCGGACCACTACACCGTCTCGGCGGCGATCACGGCTTGCGCGAAGCTACGCCATGTCGTTTTCGGGACGCAACTCCACGGCAATGCGGTTCGCTCCGGCCTCAAAGCCTTTCCTCACGTGGCGAACCCGCTGCTCTCGCTTTACGCGAGCGCGGGAGATTTGGATGACGTAAGATTGGTGTTTGATGAGATTGCAGAGCCTGATGTTTATGCGTGGACGACGCTGCTCTCGGCGTGTGCGAAACTTGGGGAGGTTGAGTATGCGTGCAAGGTGTTTGATAAAATCCCTGAGAAGAGTAATGTGGCGATTTGGAATGCGATGATTACCGGTTGCGCGGAGAACGGGGCTGAGGAGGTTGCGTTTGGTTTGTTTTGTGAAATGCGTAGGATGGGTGTGAGGTGTGATAATTATAGCGCTGCGAGTGTGTTGAGTTTGTGTTGTTTGGAGGGGATTGGGTTTGGGAGGCAGGTGCATTGTTTGGCGGTTAGGACTGGGTTGTTGGGGAGAGGTTCGGTTGTGAATGCTCTGCTTACGATGTATTTTAACTGTGGGAGTGTTGCGGAGGCGTTTGAGGTGTTTGGAGAAGCGGAAGATGGGGTGTGTGATGAGATTAGTTTTAATGTGATGATTGATGGTTTGGTGGGTGTGGGAAGAGATGAAGAGGGTTTGATAATGTTTAAAGGGATGCAAGAGGCGGGTCTGAGGCCGACTGAGCTGACTTTTGTGAGTGTCATGAGCTCGCGTTTGGCTGCGAGTGTTGCTAAGCATGTACATGCGCAGGCGATTAAGTTGGGATTTGAAGCTTTTACATCTGTGAGCAATGCAGCAATAACCACGTATACTGGCTGTGGGGATTCACAAGCGGCGTACCTAGTTTTTCATAAGTTGGAAGAGAGAGATCTTATATCATGGAATTCCATGATCTCGACTTATGCTCAAGTGAATGACAGTGAATCGGTCATTTTGGTCTACCTGCAAATGCAGAGGGCAGGAGAGAAACCAGATGAGTTTACATATGGAAGTTTGTTGGCAAGCTCTGTGTGTATTGAGACCGTGCAAATGATTCAGGCTGTTGCACACAAAAATGGGCTTATCTTTAAAATTCAAGTTTCAAATGCAATGGTTTCTGCATATGCTAAGCAGGGTCAGATGAATCTAGCCTATCAGACATTCCAAGATACCAAGTACAAAAACTTGATCACCTGGAATGCTATTATTTCTGGATTCATGCTGAATGGAATGGTAAATGAAGGGTTAGAGCAATTTCATGAGCTGCTAGTGTCAGAACTTGGTCCAGATGTGTATACCCTGAGTATAATTTTAAGCATGTGTGCTAGCATAACATCCTTGAGAGATGGAAAACCAGTTCATGCCTACATTCTCAAATTTGGGTTCTCTCAACAACTGTGCTTAGGCAATGCCCTCATTACAATGTATGCTAAATGCGGGGTTTTAGACTGGTCGGTAAGAGTGTTTGAAGCAATGAATGAAAAGGATATAGTCTCATGGAATGCCCTGATATCAGCTTATGCACAACATGGGAAAGGAAAGAAAGCTGTTGATTGTTTTGAGGCAATGCAAGACTTATCTTCTATCACACCAGACCAGGCAACCTTCACTGCAGTTCTTTCCGCTTGCAGCCATGCCGGTTTGGTTCATGATGGTACACAAATTTTCAATTCCATGATTAGTAATTACGGCTTTATGCCAGAAGTAGATCATTTTTCTTGTATAGTTGACCTTCTCGGTCGTGCCGGGTACCTTGATGAGGCAGAAACAGTGATAAACAGCAAGGAAATTGAAGCACATCCGAATATTTGGTGGACGCTAATTAGTTCCTGTGCAGCACATGGTAACTTAAGACTAGGAAGAATAGTTGCTGGATATCTCCTTGAAACTGAACAAAACAATCCATCAGTTTATGTGCTTTTGGCAAGTATATATGCAGCTGCTGGTCAATGGGAAGAAGCAGCTAATGTGAGAGAATTAATGAACAGAACTGGGGAGGTGAAGACACCTGGATGCAGTTGGATTAATTCATAA

Protein Analysis

734

Amino Acids

80.26

Weight (kDa)

5.31

Isoelectric Point (pI)

29.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 153 - 200 4.1e-09 PPR repeat family
PPR PF01535 156 - 185 5.4e-06 PPR repeat
PPR_2 PF13041 353 - 396 5.9e-08 PPR repeat family
PPR_2 PF13041 451 - 498 6e-08 PPR repeat family
PPR_2 PF13041 551 - 600 1.5e-11 PPR repeat family
PPR PF01535 554 - 581 2.8e-07 PPR repeat
E_motif PF20431 670 - 732 4.5e-17 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016025)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49740
fragaria_vesca FvH4_1g18310
malus_domestica MD02G1190400.v1.1
prunus_persica Prupe.6G216000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0108961
rosa_laevigata RLG00000017696
rosa_multiflora Rmu_sc0014080.1_g000004 Rmu_sc0038162.1_g000001
rosa_roxburghii Rroxscaffold_2G00134680
rosa_rugosa Rorug02G0156700
rosa_samantha Rh2AG207300 Rh2BG218500 Rh2CG210500 Rh2DG213500
rosa_wichuraiana Rw0G011000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 616
Acc16I TGCGCA 1 cut(s) 936
Acc36I ACCTGC 2 cut(s) 616, 1140
Acc65I GGTACC 1 cut(s) 1917
AccB1I GGYRCC 1 cut(s) 1917
AccB7I CCANNNNNTGG 1 cut(s) 79
AccBSI CCGCTC 1 cut(s) 96
AccI GTMKAC 3 cut(s) 998, 1128, 1463
AccII CGCG 5 cut(s) 200, 305, 311, 494, 901
AcsI RAATTY 4 cut(s) 1072, 1260, 1544, 1830
AcuI CTGAAG 2 cut(s) 83, 104
AcvI CACGTG 1 cut(s) 277
AfaI GTAC 6 cut(s) 60, 930, 1028, 1346, 1825, 1919
AfeI AGCGCT 1 cut(s) 574
AfiI CCNNNNNNNGG 2 cut(s) 79, 1927
AflII CTTAAG 1 cut(s) 2017
AgeI ACCGGT 1 cut(s) 485
AgsI TTSAA 8 cut(s) 10, 959, 1265, 1272, 1637, 1837, 1961, 2054
AjnI CCWGG 3 cut(s) 1361, 1764, 2176
AloI GAACNNNNNNTCC 2 cut(s) 1509, 1541
Alw21I GWGCWC 1 cut(s) 899
Alw26I GTCTC 4 cut(s) 107, 181, 1202, 1666
AlwNI CAGNNNCTG 3 cut(s) 79, 359, 2111
Aor51HI AGCGCT 1 cut(s) 574
AoxI GGCC 3 cut(s) 158, 256, 863
ApoI RAATTY 4 cut(s) 1072, 1260, 1544, 1830
AseI ATTAAT 2 cut(s) 2147, 2193
AsiGI ACCGGT 1 cut(s) 485
Asp718I GGTACC 1 cut(s) 1917
AspLEI GCGC 5 cut(s) 200, 311, 494, 575, 937
AspS9I GGNCC 2 cut(s) 163, 1451
AsuC2I CCSGG 1 cut(s) 1915
AsuHPI GGTGA 5 cut(s) 65, 94, 121, 1351, 2179
AsuNHI GCTAGC 1 cut(s) 1493
AvaII GGWCC 2 cut(s) 163, 1451
BanI GGYRCC 1 cut(s) 1917
BanII GRGCYC 1 cut(s) 899
BarI GAAGNNNNNNTAC 2 cut(s) 102, 134
BbrPI CACGTG 1 cut(s) 277
BbsI GAAGAC 2 cut(s) 19, 2177
Bbv12I GWGCWC 1 cut(s) 899
BbvCI CCTCAGC 1 cut(s) 507
BccI CCATC 6 cut(s) 541, 746, 785, 1508, 1814, 2080
BceAI ACGGC 3 cut(s) 207, 253, 1873
BcgI CGANNNNNNTGC 2 cut(s) 271, 305
BciT130I CCWGG 3 cut(s) 1363, 1766, 2178
BclI TGATCA 1 cut(s) 1356
BcnI CCSGG 1 cut(s) 1915
BcoDI GTCTC 4 cut(s) 107, 181, 1202, 1666
BfaI CTAG 5 cut(s) 1031, 1316, 1436, 1494, 2024
BfmI CTRYAG 1 cut(s) 1779
BfoI RGCGCY 1 cut(s) 576
BfrI CTTAAG 1 cut(s) 2017
BfuAI ACCTGC 2 cut(s) 616, 1140
BglII AGATCT 1 cut(s) 1057
BlpI GCTNAGC 2 cut(s) 921, 1296
BmcAI AGTACT 1 cut(s) 60
Bme1390I CCNGG 4 cut(s) 1363, 1766, 1915, 2178
Bme18I GGWCC 2 cut(s) 163, 1451
BmgT120I GGNCC 2 cut(s) 163, 1451
BmiI GGNNCC 1 cut(s) 1919
BmrFI CCNGG 4 cut(s) 1363, 1766, 1915, 2178
BmrI ACTGGG 2 cut(s) 660, 2169
BmsI GCATC 2 cut(s) 834, 2171
BmtI GCTAGC 1 cut(s) 1497
BmuI ACTGGG 2 cut(s) 660, 2169
BpiI GAAGAC 2 cut(s) 19, 2177
Bpu10I CCTNAGC 2 cut(s) 507, 1570
Bpu1102I GCTNAGC 2 cut(s) 921, 1296
BpuEI CTTGAG 2 cut(s) 1080, 1528
BpuMI CCSGG 1 cut(s) 1915
BsaAI YACGTR 3 cut(s) 109, 277, 996
BsaI GGTCTC 1 cut(s) 1202
BsaJI CCNNGG 1 cut(s) 235
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 4 cut(s) 401, 431, 503, 533
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 1927
Bse118I RCCGGY 3 cut(s) 50, 485, 1805
Bse1I ACTGG 5 cut(s) 655, 1006, 1523, 1622, 2164
Bse3DI GCAATG 6 cut(s) 247, 985, 1284, 1582, 1647, 1740
BseBI CCWGG 3 cut(s) 1363, 1766, 2178
BseDI CCNNGG 1 cut(s) 235
BseGI GGATG 6 cut(s) 328, 552, 849, 1502, 1966, 2186
BseLI CCNNNNNNNGG 2 cut(s) 79, 1927
BseMI GCAATG 6 cut(s) 247, 985, 1284, 1582, 1647, 1740
BseMII CTCAG 5 cut(s) 438, 498, 851, 861, 1460
BseNI ACTGG 5 cut(s) 655, 1006, 1523, 1622, 2164
BseRI GAGGAG 1 cut(s) 524
BsgI GTGCAG 1 cut(s) 2022
Bsh1236I CGCG 5 cut(s) 200, 305, 311, 494, 901
Bsh1285I CGRYCG 1 cut(s) 1909
BshFI GGCC 3 cut(s) 160, 258, 865
BshNI GGYRCC 1 cut(s) 1917
BshTI ACCGGT 1 cut(s) 485
BsiEI CGRYCG 1 cut(s) 1909
BsiHKAI GWGCWC 1 cut(s) 899
BsiSI CCGG 6 cut(s) 51, 161, 255, 486, 1806, 1914
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 2 cut(s) 79, 1927
BsmAI GTCTC 4 cut(s) 107, 181, 1202, 1666
BsmBI CGTCTC 2 cut(s) 107, 181
BsmFI GGGAC 1 cut(s) 238
BsmI GAATGC 4 cut(s) 478, 685, 1372, 1675
BsnI GGCC 3 cut(s) 160, 258, 865
Bso31I GGTCTC 1 cut(s) 1202
Bsp1286I GDGCHC 1 cut(s) 899
Bsp1407I TGTACA 1 cut(s) 928
Bsp143I GATC 5 cut(s) 186, 1057, 1080, 1356, 1876
Bsp1720I GCTNAGC 2 cut(s) 921, 1296
BspANI GGCC 3 cut(s) 160, 258, 865
BspCNI CTCAG 5 cut(s) 439, 499, 852, 862, 1461
BspFNI CGCG 5 cut(s) 200, 305, 311, 494, 901
BspHI TCATGA 3 cut(s) 891, 1426, 1816
BspLI GGNNCC 1 cut(s) 1919
BspMAI CTGCAG 1 cut(s) 1783
BspMI ACCTGC 2 cut(s) 616, 1140
BspOI GCTAGC 1 cut(s) 1497
BspQI GCTCTTC 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 1917
BspTI CTTAAG 1 cut(s) 2017
BspTNI GGTCTC 1 cut(s) 1202
BsrBI CCGCTC 1 cut(s) 96
BsrDI GCAATG 6 cut(s) 247, 985, 1284, 1582, 1647, 1740
BsrFI RCCGGY 3 cut(s) 50, 485, 1805
BsrGI TGTACA 1 cut(s) 928
BsrI ACTGG 5 cut(s) 655, 1006, 1523, 1622, 2164
BssAI RCCGGY 3 cut(s) 50, 485, 1805
BssECI CCNNGG 1 cut(s) 235
BssMI GATC 5 cut(s) 186, 1057, 1080, 1356, 1876
BssNAI GTATAC 2 cut(s) 999, 1464
Bst1107I GTATAC 2 cut(s) 999, 1464
Bst2UI CCWGG 3 cut(s) 1363, 1766, 2178
Bst4CI ACNGT 7 cut(s) 150, 175, 707, 1109, 1213, 1566, 1939
Bst6I CTCTTC 5 cut(s) 131, 446, 805, 813, 1044
BstAFI CTTAAG 1 cut(s) 2017
BstAUI TGTACA 1 cut(s) 928
BstBAI YACGTR 3 cut(s) 109, 277, 996
BstC8I GCNNGC 9 cut(s) 98, 196, 307, 424, 899, 939, 1192, 1495, 1795
BstDEI CTNAG 8 cut(s) 447, 507, 860, 870, 921, 1296, 1469, 1570
BstDSI CCRYGG 1 cut(s) 235
BstENI CCTNNNNNAGG 1 cut(s) 1925
BstF5I GGATG 6 cut(s) 328, 552, 849, 1502, 1966, 2186
BstFNI CGCG 5 cut(s) 200, 305, 311, 494, 901
BstH2I RGCGCY 1 cut(s) 576
BstHHI GCGC 5 cut(s) 200, 311, 494, 575, 937
BstKTI GATC 5 cut(s) 189, 1060, 1083, 1359, 1879
BstMAI GTCTC 4 cut(s) 107, 181, 1202, 1666
BstMBI GATC 5 cut(s) 186, 1057, 1080, 1356, 1876
BstMCI CGRYCG 1 cut(s) 1909
BstMWI GCNNNNNNNGC 5 cut(s) 85, 191, 295, 871, 917
BstNI CCWGG 3 cut(s) 1363, 1766, 2178
BstNSI RCATGY 3 cut(s) 929, 935, 1489
BstSCI CCNGG 4 cut(s) 1361, 1764, 1913, 2176
BstSFI CTRYAG 1 cut(s) 1779
BstSNI TACGTA 1 cut(s) 109
BstUI CGCG 5 cut(s) 200, 305, 311, 494, 901
BstV2I GAAGAC 2 cut(s) 19, 2177
BstX2I RGATCY 1 cut(s) 1057
BstYI RGATCY 1 cut(s) 1057
BstZ17I GTATAC 2 cut(s) 999, 1464
BsuRI GGCC 3 cut(s) 160, 258, 865
BtgI CCRYGG 1 cut(s) 235
BtgZI GCGATG 1 cut(s) 491
BtsCI GGATG 6 cut(s) 328, 552, 849, 1502, 1966, 2186
BtsI GCAGTG 1 cut(s) 1776
BtsIMutI CAGTG 3 cut(s) 1114, 1776, 1944
BveI ACCTGC 2 cut(s) 616, 1140
Cac8I GCNNGC 9 cut(s) 98, 196, 307, 424, 899, 939, 1192, 1495, 1795
CaiI CAGNNNCTG 3 cut(s) 79, 359, 2111
CciI TCATGA 3 cut(s) 891, 1426, 1816
CfoI GCGC 5 cut(s) 200, 311, 494, 575, 937
Cfr10I RCCGGY 3 cut(s) 50, 485, 1805
Cfr13I GGNCC 2 cut(s) 163, 1451
CseI GACGC 4 cut(s) 124, 235, 388, 1993
Csp6I GTAC 6 cut(s) 59, 929, 1027, 1345, 1824, 1918
CspAI ACCGGT 1 cut(s) 485
CviQI GTAC 6 cut(s) 59, 929, 1027, 1345, 1824, 1918
DdeI CTNAG 8 cut(s) 447, 507, 860, 870, 921, 1296, 1469, 1570
DpnI GATC 5 cut(s) 188, 1059, 1082, 1358, 1878
DpnII GATC 5 cut(s) 186, 1057, 1080, 1356, 1876
DraI TTTAAA 2 cut(s) 838, 1258
Eam1104I CTCTTC 5 cut(s) 131, 446, 805, 813, 1044
EarI CTCTTC 5 cut(s) 131, 446, 805, 813, 1044
Ecl136II GAGCTC 1 cut(s) 897
Eco105I TACGTA 1 cut(s) 109
Eco24I GRGCYC 1 cut(s) 899
Eco31I GGTCTC 1 cut(s) 1202
Eco32I GATATC 2 cut(s) 1680, 2045
Eco47I GGWCC 2 cut(s) 163, 1451
Eco47III AGCGCT 1 cut(s) 574
Eco53kI GAGCTC 1 cut(s) 897
Eco57I CTGAAG 2 cut(s) 83, 104
Eco72I CACGTG 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 897
EcoNI CCTNNNNNAGG 1 cut(s) 1925
EcoRI GAATTC 1 cut(s) 1072
EcoRII CCWGG 3 cut(s) 1361, 1764, 2176
EcoRV GATATC 2 cut(s) 1680, 2045
EcoT38I GRGCYC 1 cut(s) 899
Esp3I CGTCTC 2 cut(s) 107, 181
FaqI GGGAC 1 cut(s) 238
FauI CCCGC 4 cut(s) 294, 304, 847, 1598
FauNDI CATATG 2 cut(s) 1174, 1291
FbaI TGATCA 1 cut(s) 1356
FblI GTMKAC 3 cut(s) 998, 1128, 1463
FokI GGATG 6 cut(s) 335, 559, 856, 1489, 1953, 2193
FriOI GRGCYC 1 cut(s) 899
FspBI CTAG 5 cut(s) 1031, 1316, 1436, 1494, 2024
FspI TGCGCA 1 cut(s) 936
GlaI GCGC 5 cut(s) 199, 310, 493, 574, 936
HaeII RGCGCY 1 cut(s) 576
HaeIII GGCC 3 cut(s) 160, 258, 865
HapII CCGG 6 cut(s) 51, 161, 255, 486, 1806, 1914
HgaI GACGC 4 cut(s) 124, 235, 388, 1993
HhaI GCGC 5 cut(s) 200, 311, 494, 575, 937
Hin6I GCGC 5 cut(s) 198, 309, 492, 573, 935
HinP1I GCGC 5 cut(s) 198, 309, 492, 573, 935
HincII GTYRAC 1 cut(s) 1897
HindII GTYRAC 1 cut(s) 1897
HindIII AAGCTT 2 cut(s) 86, 960
HinfI GANTC 5 cut(s) 1013, 1112, 1222, 1312, 1383
HpaII CCGG 6 cut(s) 51, 161, 255, 486, 1806, 1914
HphI GGTGA 5 cut(s) 65, 94, 121, 1351, 2179
Hpy166II GTNNAC 8 cut(s) 375, 999, 1129, 1170, 1464, 1826, 1897, 1983
Hpy188I TCNGA 5 cut(s) 861, 1308, 1326, 1444, 1971
Hpy188III TCNNGA 8 cut(s) 31, 223, 892, 1084, 1380, 1427, 1454, 1817
Hpy8I GTNNAC 8 cut(s) 375, 999, 1129, 1170, 1464, 1826, 1897, 1983
Hpy99I CGWCG 1 cut(s) 382
HpyAV CCTTC 3 cut(s) 1403, 1783, 1910
HpyCH4III ACNGT 7 cut(s) 150, 175, 707, 1109, 1213, 1566, 1939
HpyCH4IV ACGT 4 cut(s) 108, 276, 327, 995
HpyF10VI GCNNNNNNNGC 5 cut(s) 85, 191, 295, 871, 917
HpyF3I CTNAG 8 cut(s) 447, 507, 860, 870, 921, 1296, 1469, 1570
HpySE526I ACGT 4 cut(s) 108, 276, 327, 995
HspAI GCGC 5 cut(s) 198, 309, 492, 573, 935
KpnI GGTACC 1 cut(s) 1921
Ksp22I TGATCA 1 cut(s) 1356
Kzo9I GATC 5 cut(s) 186, 1057, 1080, 1356, 1876
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 1 cut(s) 257
LweI GCATC 2 cut(s) 834, 2171
MaeI CTAG 5 cut(s) 1031, 1316, 1436, 1494, 2024
MaeII ACGT 4 cut(s) 108, 276, 327, 995
MaeIII GTNAC 2 cut(s) 104, 2012
MalI GATC 5 cut(s) 188, 1059, 1082, 1358, 1878
MbiI CCGCTC 1 cut(s) 96
MboI GATC 5 cut(s) 186, 1057, 1080, 1356, 1876
MflI RGATCY 1 cut(s) 1057
MhlI GDGCHC 1 cut(s) 899
MmeI TCCRAC 2 cut(s) 1026, 2168
MslI CAYNNNNRTG 5 cut(s) 240, 930, 1242, 1589, 2179
MspA1I CMGCKG 2 cut(s) 289, 2108
MspCI CTTAAG 1 cut(s) 2017
MspI CCGG 6 cut(s) 51, 161, 255, 486, 1806, 1914
MspR9I CCNGG 4 cut(s) 1363, 1766, 1915, 2178
Mva1269I GAATGC 4 cut(s) 478, 685, 1372, 1675
MvaI CCWGG 3 cut(s) 1363, 1766, 2178
MvnI CGCG 5 cut(s) 200, 305, 311, 494, 901
MwoI GCNNNNNNNGC 5 cut(s) 85, 191, 295, 871, 917
NciI CCSGG 1 cut(s) 1915
NdeI CATATG 2 cut(s) 1174, 1291
NdeII GATC 5 cut(s) 186, 1057, 1080, 1356, 1876
NheI GCTAGC 1 cut(s) 1493
NlaIV GGNNCC 1 cut(s) 1919
NmeAIII GCCGAG 2 cut(s) 158, 368
NsbI TGCGCA 1 cut(s) 936
NspI RCATGY 3 cut(s) 929, 935, 1489
PagI TCATGA 3 cut(s) 891, 1426, 1816
PaqCI CACCTGC 1 cut(s) 616
PciSI GCTCTTC 1 cut(s) 131
PcsI WCGNNNNNNNCGW 3 cut(s) 197, 302, 395
PctI GAATGC 4 cut(s) 478, 685, 1372, 1675
PfeI GAWTC 5 cut(s) 1013, 1112, 1222, 1312, 1383
PflMI CCANNNNNTGG 1 cut(s) 79
PinAI ACCGGT 1 cut(s) 485
PmaCI CACGTG 1 cut(s) 277
PmlI CACGTG 1 cut(s) 277
Ppu21I YACGTR 3 cut(s) 109, 277, 996
PshBI ATTAAT 2 cut(s) 2147, 2193
Psp124BI GAGCTC 1 cut(s) 899
Psp6I CCWGG 3 cut(s) 1361, 1764, 2176
PspCI CACGTG 1 cut(s) 277
PspGI CCWGG 3 cut(s) 1361, 1764, 2176
PspN4I GGNNCC 1 cut(s) 1919
PspPI GGNCC 2 cut(s) 163, 1451
PstI CTGCAG 1 cut(s) 1783
PstNI CAGNNNCTG 3 cut(s) 79, 359, 2111
PsuI RGATCY 1 cut(s) 1057
PvuII CAGCTG 1 cut(s) 2108
RsaI GTAC 6 cut(s) 60, 930, 1028, 1346, 1825, 1919
RsaNI GTAC 6 cut(s) 59, 929, 1027, 1345, 1824, 1918
RseI CAYNNNNRTG 5 cut(s) 240, 930, 1242, 1589, 2179
SacI GAGCTC 1 cut(s) 899
SapI GCTCTTC 1 cut(s) 131
Sau3AI GATC 5 cut(s) 186, 1057, 1080, 1356, 1876
Sau96I GGNCC 2 cut(s) 163, 1451
ScaI AGTACT 1 cut(s) 60
ScrFI CCNGG 4 cut(s) 1363, 1766, 1915, 2178
SduI GDGCHC 1 cut(s) 899
SfaNI GCATC 2 cut(s) 834, 2171
SfcI CTRYAG 1 cut(s) 1779
SgrAI CRCCGGYG 1 cut(s) 50
SinI GGWCC 2 cut(s) 163, 1451
SmiMI CAYNNNNRTG 5 cut(s) 240, 930, 1242, 1589, 2179
SmlI CTYRAG 3 cut(s) 1095, 1507, 2017
SmoI CTYRAG 3 cut(s) 1095, 1507, 2017
SnaBI TACGTA 1 cut(s) 109
SspI AATATT 1 cut(s) 1975
SspMI CTAG 5 cut(s) 1031, 1316, 1436, 1494, 2024
SstI GAGCTC 1 cut(s) 899
StyD4I CCNGG 4 cut(s) 1361, 1764, 1913, 2176
TaaI ACNGT 7 cut(s) 150, 175, 707, 1109, 1213, 1566, 1939
TaiI ACGT 4 cut(s) 111, 279, 330, 998
TaqI TCGA 1 cut(s) 1085
TaqII GACCGA 2 cut(s) 1105, 1895
TatI WGTACW 3 cut(s) 58, 928, 1344
TauI GCSGC 3 cut(s) 50, 185, 1025
TfiI GAWTC 5 cut(s) 1013, 1112, 1222, 1312, 1383
TscAI CASTG 3 cut(s) 1114, 1783, 1944
TspRI CASTG 3 cut(s) 1114, 1783, 1944
Van91I CCANNNNNTGG 1 cut(s) 79
Vha464I CTTAAG 1 cut(s) 2017
VpaK11BI GGWCC 2 cut(s) 163, 1451
VspI ATTAAT 2 cut(s) 2147, 2193
XagI CCTNNNNNAGG 1 cut(s) 1925
XapI RAATTY 4 cut(s) 1072, 1260, 1544, 1830
XceI RCATGY 3 cut(s) 929, 935, 1489
XmiI GTMKAC 3 cut(s) 998, 1128, 1463
XspI CTAG 5 cut(s) 1031, 1316, 1436, 1494, 2024
ZrmI AGTACT 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.