Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0016025

Physical Location & Seq

Genomic Coordinates Reverse (-)

17547762 .. 17550007

Gene Structure 2246 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1190400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2205 bp

ATGAGGTTCCACTTCTACAAAGAACCCACAAAAACCCTTACCCAAGTCGCCACTAAGCAGATCCTCAAACTGAACCAAACGCTCGCCAACCTCACGCGCTCGCACCGTTACTCAGAGGCCCTCCAGCTTTTCACCCAAATCCACTCCTCTCCGTCCGTAGGACCGGACCACTACACGCTCTCATCTGCCGTCACCGCCTGCGCCAATTGCCGCGACGTTGCTTTCGGAACCCAACTCCATGCCCACGCCATCCGGACTGGCCTTAAAGCCTACCCTCACGTAGCCAACACCCTGCTCTCGCTCTATGCGAAAGCCGAAGACTTGACTTCTGTGAAATGGGTCTTCGACGGGATTGAAAACCCAGATGTTTATTCGTGGACCACGCTGTTTTCGGCTTGTACAAAATTGGGGCATGTGGATTATGCCTGTGAGGTGTTTGATAAAATGCCCCAAAAGGGTAGTGTGGCGATTTGGAATGCGATGATTACCGGGTGTGCGGAGAATGGGTTTGATGTGGTTGCTATTGATTTTTTTTGTGAAATGCATAGGATGGGTGTTATGCATGATAATTATGGTTTTGCTAGTGTTTTGAGTTTGTGTGGTTTGGGGCTCTTAGGTTTTGGGAGGCAGGTGCATTCTTTAGTGCTTAAAACCGGGTTTTTGGGTAGAAGTTCTGTTGTTAATGCTCTGATAACTATGTATTTTAACTGCAGAAGTGTTTCGGAGGCATTTGATTTATTTGAAGAAGCGGAAGGTGCAATATATGATCAGATTACTTTTAATGTGATGATAGATGGTTTGGTGAGTGCCGGAAGAGATAAAGAAGCTTTGGAAATGTTCAAATTGATGCAAGAGGCATGTCTTAGGCCTACCGAGCTGACTTTTGTAAGTGTCATGACCTCATGTTCAACTGCAAGAGTTGCCAACCATGTACTTGCCCAAGCTGTTAAGTCGGGTTTCGAAACTTTTACTTCTGTGAGCAATGCAGCAATAACCATGTATTCTGGTTGTGGGGATTTGCATGCTGCTTACCAGGTTTTTCAGACATTGGAGGAGAAGGATCTCATATCATGGAACACCATGATCTCAACTTATGCTCAAGGGAATGACAGTAAATTAGCCATCTTGGTCTACCAGCAAATGCAGAGGGCAGGGGTCAAACCAGATGAGTTTACTTTTGGAAGTTTGTTAGCAAGCTCGGAGTTGATTGAGATCGTGCAAATGGCTCAAGCCCTTGCACACAAAAATGGGCTTATCCTTAAGATCTACGTTTCAAATGCATTGGTATCTGCATATGCTAAGCTGGGTCTGATTGATCTGGCCTATCCAGTATTCGAAGATATCGAGTCCAAAAATTTGATCTCCTGGAATGCTATTGTTTCCGGATTCCTGTTGAATGGATTGGTAAAAGAAGGGCTAGAGCAATTTTCTAATCTACTAATGTCAGAACACAGGCCAGATGTGTGTACCTTGAGTATTATTTTGAGCATTTGTTCAAGCACATCAGCCTTGAAAGACGGAAAACAAGTTCATGGCTACATTCTCAAATTTGGGTTTTCGCATCAGATGTGCTTAGGCAATGCCCTCATCACCATGTACGCTAAATGCGGGGTTATACATTGGTCCGTAAGAGTTTTCAATGCAATGACTCAAAAGGATACAGTCTCTTGGAATTCCCTGATAGCTGCTTATGCACAGCATGGGAAAGGAAATGAAGCTGTGCGCTGTTTTGAGGCAATGCAAGACGCATCTGCAGTCAAACCAGACCAGGCCACCTTTACGGCAGTCCTTTCCGCTTGCAGCCATGCTGGTCTAGTTGTTGATGGTACCCGAATTTTCAATTCCATGATTCGTAATTATGGCTTTATACCTGAAGTTGATCACTTTTCATGTATAGTTGACCTTCTAGGTCGTGCTGGGTATCTTGATGAGGCGGAAACTGTAATAAACAGCAAGCAAATTGAAGCACATCCAAACATTTGGTGGACACTAATCAGTTCTTGTGCAGCACATGGAAATTTAAGGCTAGGACGAACAGTTGCTGGGTTTCTCCTTGAAACTGAACAAAATAATCCAACAGTTTACGTGCTTCTGGCCAGTATTTATGCAGCTGCTGGTCAATGGGAAGAAGCAGCTAATGTGAGAGAATTGATGAACAGAACTCGGGTTCCGAAGATTCCTGGATTGAGTTGGATTAAGTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

735

Amino Acids

80.79

Weight (kDa)

6.15

Isoelectric Point (pI)

33.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	256 - 302	6.4e-07	PPR repeat family
PPR	PF01535	258 - 286	2.5e-06	PPR repeat
PPR_2	PF13041	354 - 397	8.1e-10	PPR repeat family
PPR_2	PF13041	552 - 601	1.3e-11	PPR repeat family
PPR	PF01535	555 - 582	1.2e-06	PPR repeat
E_motif	PF20431	671 - 733	6.2e-15	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49740
fragaria_vesca	FvH4_1g18310
malus_domestica	MD02G1190400.v1.1
prunus_persica	Prupe.6G216000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0108961
rosa_laevigata	RLG00000017696
rosa_multiflora	Rmu_sc0014080.1_g000004 Rmu_sc0038162.1_g000001
rosa_roxburghii	Rroxscaffold_2G00134680
rosa_rugosa	Rorug02G0156700
rosa_samantha	Rh2AG207300 Rh2BG218500 Rh2CG210500 Rh2DG213500
rosa_wichuraiana	Rw0G011000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	619
Acc36I	ACCTGC	1 cut(s)	619
Acc65I	GGTACC	1 cut(s)	1826
AccB1I	GGYRCC	1 cut(s)	1826
AccI	GTMKAC	1 cut(s)	1131
AccII	CGCG	2 cut(s)	97, 213
AccIII	TCCGGA	2 cut(s)	252, 1382
AciI	CCGC	7 cut(s)	195, 211, 497, 749, 1608, 1794, 1934
AclWI	GGATC	2 cut(s)	55, 1068
AcoI	YGGCCR	1 cut(s)	2094
AcsI	RAATTY	5 cut(s)	1354, 1547, 1672, 1833, 2017
AcuI	CTGAAG	1 cut(s)	1893
AfaI	GTAC	5 cut(s)	400, 933, 1468, 1598, 1828
AfiI	CCNNNNNNNGG	2 cut(s)	158, 455
AflII	CTTAAG	1 cut(s)	1259
AjnI	CCWGG	4 cut(s)	1032, 1364, 1767, 2179
AloI	GAACNNNNNNTCC	4 cut(s)	1512, 1544, 2152, 2184
Alw26I	GTCTC	1 cut(s)	1669
AlwI	GGATC	2 cut(s)	55, 1068
AlwNI	CAGNNNCTG	2 cut(s)	2042, 2114
Ama87I	CYCGRG	1 cut(s)	2163
Aor13HI	TCCGGA	2 cut(s)	252, 1382
AoxI	GGCC	7 cut(s)	117, 259, 866, 1320, 1454, 1770, 2094
ApeKI	GCWGC	8 cut(s)	986, 1025, 1685, 1800, 2006, 2108, 2111, 2132
ApoI	RAATTY	5 cut(s)	1354, 1547, 1672, 1833, 2017
ArsI	GACNNNNNNTTYG	2 cut(s)	206, 238
Asp700I	GAANNNNTTC	1 cut(s)	718
Asp718I	GGTACC	1 cut(s)	1826
AspLEI	GCGC	3 cut(s)	99, 203, 1725
AspS9I	GGNCC	5 cut(s)	118, 161, 166, 378, 1623
AsuC2I	CCSGG	2 cut(s)	490, 655
AsuHPI	GGTGA	4 cut(s)	124, 184, 814, 1582
AsuII	TTCGAA	2 cut(s)	960, 1335
AvaI	CYCGRG	1 cut(s)	2163
AvaII	GGWCC	4 cut(s)	161, 166, 378, 1623
BalI	TGGCCA	1 cut(s)	2096
BanI	GGYRCC	1 cut(s)	1826
BanII	GRGCYC	1 cut(s)	612
BbsI	GAAGAC	2 cut(s)	324, 334
BbvI	GCAGC	8 cut(s)	998, 1012, 1672, 1812, 2018, 2098, 2120, 2144
BccI	CCATC	5 cut(s)	257, 544, 788, 1130, 1817
BceAI	ACGGC	2 cut(s)	173, 1797
BciT130I	CCWGG	4 cut(s)	1034, 1366, 1769, 2181
BciVI	GTATCC	1 cut(s)	1651
BclI	TGATCA	2 cut(s)	766, 1879
BcnI	CCSGG	2 cut(s)	490, 655
BcoDI	GTCTC	1 cut(s)	1669
BfaI	CTAG	5 cut(s)	582, 1418, 1814, 1907, 2027
BfmI	CTRYAG	2 cut(s)	709, 1752
BfrI	CTTAAG	1 cut(s)	1259
BfuAI	ACCTGC	1 cut(s)	619
BfuI	GTATCC	1 cut(s)	1651
BglII	AGATCT	1 cut(s)	1263
BisI	GCNGC	9 cut(s)	211, 987, 1026, 1686, 1801, 2007, 2109, 2112, 2133
BlpI	GCTNAGC	1 cut(s)	1299
BlsI	GCNGC	9 cut(s)	212, 988, 1027, 1687, 1802, 2008, 2110, 2113, 2134
Bme1390I	CCNGG	6 cut(s)	490, 655, 1034, 1366, 1769, 2181
Bme18I	GGWCC	4 cut(s)	161, 166, 378, 1623
BmeT110I	CYCGRG	1 cut(s)	2163
BmgT120I	GGNCC	5 cut(s)	118, 161, 166, 378, 1623
BmiI	GGNNCC	4 cut(s)	8, 229, 1828, 2169
BmrFI	CCNGG	6 cut(s)	490, 655, 1034, 1366, 1769, 2181
BmsI	GCATC	3 cut(s)	837, 1570, 1757
BpiI	GAAGAC	2 cut(s)	324, 334
BpmI	CTGGAG	1 cut(s)	107
Bpu10I	CCTNAGC	1 cut(s)	1573
Bpu1102I	GCTNAGC	1 cut(s)	1299
Bpu14I	TTCGAA	2 cut(s)	960, 1335
BpuEI	CTTGAG	3 cut(s)	1083, 1212, 1492
BpuMI	CCSGG	2 cut(s)	490, 655
BsaAI	YACGTR	2 cut(s)	280, 2086
BsaBI	GATNNNNATC	1 cut(s)	1211
BsaWI	WCCGGW	3 cut(s)	163, 252, 1382
BsaXI	ACNNNNNCTCC	2 cut(s)	491, 521
Bsc4I	CCNNNNNNNGG	2 cut(s)	158, 455
Bse1I	ACTGG	3 cut(s)	262, 1328, 2097
Bse3DI	GCAATG	4 cut(s)	988, 1585, 1650, 1743
Bse8I	GATNNNNATC	1 cut(s)	1211
BseAI	TCCGGA	2 cut(s)	252, 1382
BseBI	CCWGG	4 cut(s)	1034, 1366, 1769, 2181
BseGI	GGATG	3 cut(s)	249, 555, 1969
BseJI	GATNNNNATC	1 cut(s)	1211
BseLI	CCNNNNNNNGG	2 cut(s)	158, 455
BseMI	GCAATG	4 cut(s)	988, 1585, 1650, 1743
BseMII	CTCAG	1 cut(s)	126
BseNI	ACTGG	3 cut(s)	262, 1328, 2097
BseRI	GAGGAG	2 cut(s)	136, 1067
BseXI	GCAGC	8 cut(s)	998, 1012, 1672, 1812, 2018, 2098, 2120, 2144
BseYI	CCCAGC	3 cut(s)	1303, 1916, 2042
BsgI	GTGCAG	1 cut(s)	2025
Bsh1236I	CGCG	2 cut(s)	97, 213
BshFI	GGCC	7 cut(s)	119, 261, 868, 1322, 1456, 1772, 2096
BshNI	GGYRCC	1 cut(s)	1826
BsiHKCI	CYCGRG	1 cut(s)	2163
BsiSI	CCGG	6 cut(s)	164, 253, 489, 654, 810, 1383
BslI	CCNNNNNNNGG	2 cut(s)	158, 455
BsmAI	GTCTC	1 cut(s)	1669
BsmI	GAATGC	3 cut(s)	481, 634, 1375
BsnI	GGCC	7 cut(s)	119, 261, 868, 1322, 1456, 1772, 2096
BsoBI	CYCGRG	1 cut(s)	2163
Bsp119I	TTCGAA	2 cut(s)	960, 1335
Bsp1286I	GDGCHC	1 cut(s)	612
Bsp13I	TCCGGA	2 cut(s)	252, 1382
Bsp1407I	TGTACA	1 cut(s)	398
Bsp143I	GATC	9 cut(s)	60, 766, 1060, 1083, 1212, 1263, 1315, 1359, 1879
Bsp1720I	GCTNAGC	1 cut(s)	1299
BspACI	CCGC	7 cut(s)	195, 211, 497, 749, 1608, 1794, 1934
BspANI	GGCC	7 cut(s)	119, 261, 868, 1322, 1456, 1772, 2096
BspCNI	CTCAG	1 cut(s)	125
BspEI	TCCGGA	2 cut(s)	252, 1382
BspFNI	CGCG	2 cut(s)	97, 213
BspHI	TCATGA	1 cut(s)	894
BspLI	GGNNCC	4 cut(s)	8, 229, 1828, 2169
BspMAI	CTGCAG	2 cut(s)	713, 1756
BspMI	ACCTGC	1 cut(s)	619
BspPI	GGATC	2 cut(s)	55, 1068
BspT104I	TTCGAA	2 cut(s)	960, 1335
BspT107I	GGYRCC	1 cut(s)	1826
BspTI	CTTAAG	1 cut(s)	1259
BsrDI	GCAATG	4 cut(s)	988, 1585, 1650, 1743
BsrGI	TGTACA	1 cut(s)	398
BsrI	ACTGG	3 cut(s)	262, 1328, 2097
BssMI	GATC	9 cut(s)	60, 766, 1060, 1083, 1212, 1263, 1315, 1359, 1879
Bst2UI	CCWGG	4 cut(s)	1034, 1366, 1769, 2181
Bst4CI	ACNGT	6 cut(s)	107, 1112, 1663, 1942, 2038, 2080
Bst6I	CTCTTC	1 cut(s)	808
BstAFI	CTTAAG	1 cut(s)	1259
BstAPI	GCANNNNNTGC	1 cut(s)	920
BstAUI	TGTACA	1 cut(s)	398
BstBAI	YACGTR	2 cut(s)	280, 2086
BstBI	TTCGAA	2 cut(s)	960, 1335
BstC8I	GCNNGC	7 cut(s)	84, 101, 199, 1023, 1195, 1798, 1955
BstDEI	CTNAG	6 cut(s)	54, 112, 613, 863, 1299, 1573
BstF5I	GGATG	3 cut(s)	249, 555, 1969
BstFNI	CGCG	2 cut(s)	97, 213
BstHHI	GCGC	3 cut(s)	99, 203, 1725
BstKTI	GATC	9 cut(s)	63, 769, 1063, 1086, 1215, 1266, 1318, 1362, 1882
BstMAI	GTCTC	1 cut(s)	1669
BstMBI	GATC	9 cut(s)	60, 766, 1060, 1083, 1212, 1263, 1315, 1359, 1879
BstMWI	GCNNNNNNNGC	6 cut(s)	194, 207, 755, 874, 920, 1691
BstNI	CCWGG	4 cut(s)	1034, 1366, 1769, 2181
BstNSI	RCATGY	3 cut(s)	416, 861, 1025
BstSCI	CCNGG	6 cut(s)	488, 653, 1032, 1364, 1767, 2179
BstSFI	CTRYAG	2 cut(s)	709, 1752
BstUI	CGCG	2 cut(s)	97, 213
BstV1I	GCAGC	8 cut(s)	998, 1012, 1672, 1812, 2018, 2098, 2120, 2144
BstV2I	GAAGAC	2 cut(s)	324, 334
BstX2I	RGATCY	3 cut(s)	60, 1060, 1263
BstXI	CCANNNNNNTGG	1 cut(s)	1980
BstYI	RGATCY	3 cut(s)	60, 1060, 1263
BsuI	GTATCC	1 cut(s)	1651
BsuRI	GGCC	7 cut(s)	119, 261, 868, 1322, 1456, 1772, 2096
BtgZI	GCGATG	1 cut(s)	494
BtsCI	GGATG	3 cut(s)	249, 555, 1969
BveI	ACCTGC	1 cut(s)	619
Cac8I	GCNNGC	7 cut(s)	84, 101, 199, 1023, 1195, 1798, 1955
CaiI	CAGNNNCTG	2 cut(s)	2042, 2114
CciI	TCATGA	1 cut(s)	894
CfoI	GCGC	3 cut(s)	99, 203, 1725
Cfr13I	GGNCC	5 cut(s)	118, 161, 166, 378, 1623
CseI	GACGC	1 cut(s)	1754
CsiI	ACCWGGT	1 cut(s)	1032
Csp6I	GTAC	5 cut(s)	399, 932, 1467, 1597, 1827
CviQI	GTAC	5 cut(s)	399, 932, 1467, 1597, 1827
DdeI	CTNAG	6 cut(s)	54, 112, 613, 863, 1299, 1573
DpnI	GATC	9 cut(s)	62, 768, 1062, 1085, 1214, 1265, 1317, 1361, 1881
DpnII	GATC	9 cut(s)	60, 766, 1060, 1083, 1212, 1263, 1315, 1359, 1879
EaeI	YGGCCR	1 cut(s)	2094
Eam1104I	CTCTTC	1 cut(s)	808
EarI	CTCTTC	1 cut(s)	808
EciI	GGCGGA	1 cut(s)	1949
Eco147I	AGGCCT	1 cut(s)	868
Eco24I	GRGCYC	1 cut(s)	612
Eco32I	GATATC	1 cut(s)	1342
Eco47I	GGWCC	4 cut(s)	161, 166, 378, 1623
Eco57I	CTGAAG	1 cut(s)	1893
Eco88I	CYCGRG	1 cut(s)	2163
EcoO109I	RGGNCCY	1 cut(s)	118
EcoRI	GAATTC	1 cut(s)	1672
EcoRII	CCWGG	4 cut(s)	1032, 1364, 1767, 2179
EcoRV	GATATC	1 cut(s)	1342
EcoT22I	ATGCAT	3 cut(s)	546, 564, 1282
EcoT38I	GRGCYC	1 cut(s)	612
FauI	CCCGC	1 cut(s)	1601
FauNDI	CATATG	1 cut(s)	1294
FbaI	TGATCA	2 cut(s)	766, 1879
FblI	GTMKAC	1 cut(s)	1131
Fnu4HI	GCNGC	9 cut(s)	211, 987, 1026, 1686, 1801, 2007, 2109, 2112, 2133
FokI	GGATG	3 cut(s)	236, 562, 1956
FriOI	GRGCYC	1 cut(s)	612
Fsp4HI	GCNGC	9 cut(s)	211, 987, 1026, 1686, 1801, 2007, 2109, 2112, 2133
FspBI	CTAG	5 cut(s)	582, 1418, 1814, 1907, 2027
GlaI	GCGC	3 cut(s)	98, 202, 1724
GluI	GCNGC	9 cut(s)	211, 987, 1026, 1686, 1801, 2007, 2109, 2112, 2133
GsaI	CCCAGC	3 cut(s)	1307, 1920, 2046
GsuI	CTGGAG	1 cut(s)	107
HaeIII	GGCC	7 cut(s)	119, 261, 868, 1322, 1456, 1772, 2096
HapII	CCGG	6 cut(s)	164, 253, 489, 654, 810, 1383
HgaI	GACGC	1 cut(s)	1754
HhaI	GCGC	3 cut(s)	99, 203, 1725
Hin6I	GCGC	3 cut(s)	97, 201, 1723
HinP1I	GCGC	3 cut(s)	97, 201, 1723
HincII	GTYRAC	1 cut(s)	1900
HindII	GTYRAC	1 cut(s)	1900
HindIII	AAGCTT	1 cut(s)	825
HinfI	GANTC	5 cut(s)	1346, 1386, 1648, 1849, 2176
HpaII	CCGG	6 cut(s)	164, 253, 489, 654, 810, 1383
HphI	GGTGA	4 cut(s)	124, 184, 814, 1582
Hpy166II	GTNNAC	7 cut(s)	378, 1132, 1173, 1467, 1900, 1986, 2083
Hpy188III	TCNNGA	4 cut(s)	253, 895, 1383, 1925
Hpy8I	GTNNAC	7 cut(s)	378, 1132, 1173, 1467, 1900, 1986, 2083
Hpy99I	CGWCG	2 cut(s)	218, 350
HpyAV	CCTTC	4 cut(s)	746, 1051, 1406, 1913
HpyCH4III	ACNGT	6 cut(s)	107, 1112, 1663, 1942, 2038, 2080
HpyCH4IV	ACGT	4 cut(s)	216, 279, 1269, 2085
HpyF10VI	GCNNNNNNNGC	6 cut(s)	194, 207, 755, 874, 920, 1691
HpyF3I	CTNAG	6 cut(s)	54, 112, 613, 863, 1299, 1573
HpySE526I	ACGT	4 cut(s)	216, 279, 1269, 2085
HspAI	GCGC	3 cut(s)	97, 201, 1723
Kpn2I	TCCGGA	2 cut(s)	252, 1382
KpnI	GGTACC	1 cut(s)	1830
Ksp22I	TGATCA	2 cut(s)	766, 1879
Kzo9I	GATC	9 cut(s)	60, 766, 1060, 1083, 1212, 1263, 1315, 1359, 1879
Lsp1109I	GCAGC	8 cut(s)	998, 1012, 1672, 1812, 2018, 2098, 2120, 2144
LweI	GCATC	3 cut(s)	837, 1570, 1757
MabI	ACCWGGT	1 cut(s)	1032
MaeI	CTAG	5 cut(s)	582, 1418, 1814, 1907, 2027
MaeII	ACGT	4 cut(s)	216, 279, 1269, 2085
MaeIII	GTNAC	2 cut(s)	107, 190
MalI	GATC	9 cut(s)	62, 768, 1062, 1085, 1214, 1265, 1317, 1361, 1881
MboI	GATC	9 cut(s)	60, 766, 1060, 1083, 1212, 1263, 1315, 1359, 1879
MboII	GAAGA	7 cut(s)	329, 334, 755, 825, 1349, 2138, 2185
MfeI	CAATTG	1 cut(s)	205
MflI	RGATCY	3 cut(s)	60, 1060, 1263
MhlI	GDGCHC	1 cut(s)	612
MlsI	TGGCCA	1 cut(s)	2096
MluNI	TGGCCA	1 cut(s)	2096
MlyI	GAGTC	2 cut(s)	1355, 1642
MmeI	TCCRAC	2 cut(s)	2099, 2171
Mox20I	TGGCCA	1 cut(s)	2096
Mph1103I	ATGCAT	3 cut(s)	546, 564, 1282
MroI	TCCGGA	2 cut(s)	252, 1382
MroXI	GAANNNNTTC	1 cut(s)	718
MscI	TGGCCA	1 cut(s)	2096
MseI	TTAA	9 cut(s)	264, 648, 681, 705, 780, 948, 1260, 2021, 2196
MslI	CAYNNNNRTG	2 cut(s)	1245, 1592
Msp20I	TGGCCA	1 cut(s)	2096
MspA1I	CMGCKG	1 cut(s)	2111
MspCI	CTTAAG	1 cut(s)	1259
MspI	CCGG	6 cut(s)	164, 253, 489, 654, 810, 1383
MspR9I	CCNGG	6 cut(s)	490, 655, 1034, 1366, 1769, 2181
MunI	CAATTG	1 cut(s)	205
Mva1269I	GAATGC	3 cut(s)	481, 634, 1375
MvaI	CCWGG	4 cut(s)	1034, 1366, 1769, 2181
MvnI	CGCG	2 cut(s)	97, 213
MwoI	GCNNNNNNNGC	6 cut(s)	194, 207, 755, 874, 920, 1691
NciI	CCSGG	2 cut(s)	490, 655
NdeI	CATATG	1 cut(s)	1294
NdeII	GATC	9 cut(s)	60, 766, 1060, 1083, 1212, 1263, 1315, 1359, 1879
NlaIV	GGNNCC	4 cut(s)	8, 229, 1828, 2169
NmuCI	GTSAC	1 cut(s)	190
NsiI	ATGCAT	3 cut(s)	546, 564, 1282
NspI	RCATGY	3 cut(s)	416, 861, 1025
NspV	TTCGAA	2 cut(s)	960, 1335
PaeI	GCATGC	1 cut(s)	1025
PagI	TCATGA	1 cut(s)	894
PaqCI	CACCTGC	1 cut(s)	619
PceI	AGGCCT	1 cut(s)	868
PcsI	WCGNNNNNNNCGW	2 cut(s)	305, 1341
PctI	GAATGC	3 cut(s)	481, 634, 1375
PdmI	GAANNNNTTC	1 cut(s)	718
PfeI	GAWTC	3 cut(s)	1386, 1849, 2176
PfoI	TCCNGGA	2 cut(s)	1364, 2179
PkrI	GCNGC	9 cut(s)	212, 988, 1027, 1687, 1802, 2008, 2110, 2113, 2134
PleI	GAGTC	2 cut(s)	1354, 1642
PpsI	GAGTC	2 cut(s)	1354, 1642
Ppu21I	YACGTR	2 cut(s)	280, 2086
Psp6I	CCWGG	4 cut(s)	1032, 1364, 1767, 2179
PspFI	CCCAGC	3 cut(s)	1303, 1916, 2042
PspGI	CCWGG	4 cut(s)	1032, 1364, 1767, 2179
PspN4I	GGNNCC	4 cut(s)	8, 229, 1828, 2169
PspPI	GGNCC	5 cut(s)	118, 161, 166, 378, 1623
PstI	CTGCAG	2 cut(s)	713, 1756
PstNI	CAGNNNCTG	2 cut(s)	2042, 2114
PsuI	RGATCY	3 cut(s)	60, 1060, 1263
PvuII	CAGCTG	1 cut(s)	2111
RsaI	GTAC	5 cut(s)	400, 933, 1468, 1598, 1828
RsaNI	GTAC	5 cut(s)	399, 932, 1467, 1597, 1827
RseI	CAYNNNNRTG	2 cut(s)	1245, 1592
SaqAI	TTAA	9 cut(s)	264, 648, 681, 705, 780, 948, 1260, 2021, 2196
SatI	GCNGC	9 cut(s)	211, 987, 1026, 1686, 1801, 2007, 2109, 2112, 2133
Sau3AI	GATC	9 cut(s)	60, 766, 1060, 1083, 1212, 1263, 1315, 1359, 1879
Sau96I	GGNCC	5 cut(s)	118, 161, 166, 378, 1623
SchI	GAGTC	2 cut(s)	1355, 1642
ScrFI	CCNGG	6 cut(s)	490, 655, 1034, 1366, 1769, 2181
SduI	GDGCHC	1 cut(s)	612
SexAI	ACCWGGT	1 cut(s)	1032
SfaNI	GCATC	3 cut(s)	837, 1570, 1757
SfcI	CTRYAG	2 cut(s)	709, 1752
SfuI	TTCGAA	2 cut(s)	960, 1335
SinI	GGWCC	4 cut(s)	161, 166, 378, 1623
SmiMI	CAYNNNNRTG	2 cut(s)	1245, 1592
SmlI	CTYRAG	4 cut(s)	1098, 1227, 1259, 1471
SmoI	CTYRAG	4 cut(s)	1098, 1227, 1259, 1471
SphI	GCATGC	1 cut(s)	1025
SseBI	AGGCCT	1 cut(s)	868
SsiI	CCGC	7 cut(s)	195, 211, 497, 749, 1608, 1794, 1934
SspMI	CTAG	5 cut(s)	582, 1418, 1814, 1907, 2027
StuI	AGGCCT	1 cut(s)	868
StyD4I	CCNGG	6 cut(s)	488, 653, 1032, 1364, 1767, 2179
TaaI	ACNGT	6 cut(s)	107, 1112, 1663, 1942, 2038, 2080
TaiI	ACGT	4 cut(s)	219, 282, 1272, 2088
TaqI	TCGA	4 cut(s)	345, 960, 1335, 1344
TatI	WGTACW	2 cut(s)	398, 931
TauI	GCSGC	1 cut(s)	213
TfiI	GAWTC	3 cut(s)	1386, 1849, 2176
Tru1I	TTAA	9 cut(s)	264, 648, 681, 705, 780, 948, 1260, 2021, 2196
Tru9I	TTAA	9 cut(s)	264, 648, 681, 705, 780, 948, 1260, 2021, 2196
TseFI	GTSAC	1 cut(s)	190
TseI	GCWGC	8 cut(s)	986, 1025, 1685, 1800, 2006, 2108, 2111, 2132
Tsp45I	GTSAC	1 cut(s)	190
TspDTI	ATGAA	4 cut(s)	1520, 1728, 1878, 2168
TspGWI	ACGGA	4 cut(s)	141, 145, 1533, 1615
Vha464I	CTTAAG	1 cut(s)	1259
VpaK11BI	GGWCC	4 cut(s)	161, 166, 378, 1623
XapI	RAATTY	5 cut(s)	1354, 1547, 1672, 1833, 2017
XceI	RCATGY	3 cut(s)	416, 861, 1025
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1980
XmiI	GTMKAC	1 cut(s)	1131
XmnI	GAANNNNTTC	1 cut(s)	718
XspI	CTAG	5 cut(s)	582, 1418, 1814, 1907, 2027
Zsp2I	ATGCAT	3 cut(s)	546, 564, 1282

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.