FvH4_1g20160

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015520
Physical Location & Seq
Forward (+)
12366418 .. 12368374
1957 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20160.t1

Sequence Viewer

Length: 1008 bp
ATGGCATTAGAATCTGTGGTGTTCCAGTCAGACCCATCTAGCTATGGAAATTTTCTAGGAGGAAGTAGATCTTGGGACCATGGCTTTGGTTTCGGAGGAGATGATGATCATCATCAAGAAGAAGGCTACGCTCAACATGATATGGTCAGCAATGGTAATACTGTAGGGCAAGGCCTTATGGGGTACTGTACACCGTCTCCGGTGGTGAAGCAAAGTGTCTACAACGACAATTCTAATTCCAATTCCAAGCCGGAAACCTGCACCGACAACTTCAACTCCCACAATTCTTTCTCTAGCCCTATCGCCGTCGCAGGCCGGAGAAAGAGGCGGCGCACGAGAAATATCAAGAACAAGGAAGAGGTGGAGAGTCAGAGGATCACCCACATTGCTGTTGAACGCAATCGGAGGAAGCTCATGAATGACTATCTGTCCGTACTGCGTTCTATGATGCCTGCTTCCTATTCCCAAAGGGGTGACCAAGCATCAATCATAGGAGGAGCCATAAATTTTGTGAAGGAGCTTGAGCAACTTTTGCAATCTTTAGAAGCCAGCAGAAGAACCAACCAACAATCAGATTTGGATTTGGCTTCCATATTCTCCAGCTTTTTCACCTTCCCTCAGTACTCGACTTGTTCGAATAAGAACACCCATGGCCATGGCCATAGCGAGTACATGAACAACAACTTGGTGCATCCCCACGAGATAACGGGGGATAAGCAGTCGGGGGCAATTGCAGATGTGGAAGTAACAATGGTAGAAAGCCATGCGAACATCAAAGTGCTTTTGAAAAGACACCCAAGACAGCTTTTAAAGATGGTGCTTGGGTTGCACTCTCTGCGTCTTGTGATCCTTCACCTTAACGTTACAAGCATGGATAGCATGATCCTCTACTCTTTCAGCGTCAAGGTTGAAGATAATTCTCAGCTGACCTCAATGAATGAGATTGCAGCTGATGTGTATGAAATGGTGGGAAGGATTCAAGAAGAGGCCGAGTTCTTACCTCAATAA

Protein Analysis

336

Amino Acids

37.56

Weight (kDa)

6.2

Isoelectric Point (pI)

60.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 124 - 175 1.8e-11 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015520)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20160 FvH4_1g20160
malus_domestica MD01G1037100.v1.1
prunus_persica Prupe.6G194400_v2.0.a1
pyrus_communis pycom01g06400
rosa_chinensis RchiOBHm_Chr2g0112221
rosa_laevigata RLG00000017960
rosa_multiflora Rmu_sc0001386.1_g000016 Rmu_sc0004467.1_g000007
rosa_roxburghii Rroxscaffold_2G00131650
rosa_rugosa Rorug02G0179300
rosa_samantha Rh2AG231500 Rh2BG244700 Rh2CG235900 Rh2DG239400
rosa_wichuraiana Rw2G017940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 266
AccI GTMKAC 1 cut(s) 219
AciI CCGC 1 cut(s) 328
AclI AACGTT 1 cut(s) 861
AclWI GGATC 3 cut(s) 383, 841, 877
AcoI YGGCCR 2 cut(s) 652, 658
AcsI RAATTY 2 cut(s) 49, 505
AfaI GTAC 5 cut(s) 185, 190, 435, 623, 671
AgsI TTSAA 5 cut(s) 274, 395, 787, 911, 980
AhdI GACNNNNNGTC 1 cut(s) 427
AjuI GAANNNNNNNTTGG 4 cut(s) 55, 87, 668, 700
AluBI AGCT 7 cut(s) 42, 412, 520, 603, 805, 925, 950
AluI AGCT 7 cut(s) 42, 412, 520, 603, 805, 925, 950
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 3 cut(s) 383, 841, 877
AoxI GGCC 5 cut(s) 172, 313, 652, 658, 987
ApeKI GCWGC 1 cut(s) 947
ApoI RAATTY 2 cut(s) 49, 505
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AspLEI GCGC 1 cut(s) 333
AspS9I GGNCC 1 cut(s) 76
AsuHPI GGTGA 5 cut(s) 217, 370, 485, 601, 845
AsuII TTCGAA 1 cut(s) 635
AvaII GGWCC 1 cut(s) 76
BalI TGGCCA 2 cut(s) 654, 660
BauI CACGAG 2 cut(s) 334, 698
BbvI GCAGC 1 cut(s) 959
BccI CCATC 2 cut(s) 43, 808
BceAI ACGGC 1 cut(s) 290
BclI TGATCA 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 3 cut(s) 39, 56, 294
BfmI CTRYAG 1 cut(s) 162
BfuAI ACCTGC 1 cut(s) 266
BglII AGATCT 1 cut(s) 68
BisI GCNGC 2 cut(s) 329, 948
BlsI GCNGC 2 cut(s) 330, 949
BmcAI AGTACT 1 cut(s) 623
Bme18I GGWCC 1 cut(s) 76
BmeRI GACNNNNNGTC 1 cut(s) 427
BmgT120I GGNCC 1 cut(s) 76
BmiI GGNNCC 2 cut(s) 77, 499
BmsI GCATC 3 cut(s) 438, 491, 700
BpmI CTGGAG 1 cut(s) 583
Bpu14I TTCGAA 1 cut(s) 635
BpuEI CTTGAG 1 cut(s) 542
BsaBI GATNNNNATC 3 cut(s) 105, 108, 111
BsaJI CCNNGG 3 cut(s) 79, 649, 655
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 4 cut(s) 181, 211, 260, 290
Bse1I ACTGG 1 cut(s) 25
Bse3DI GCAATG 2 cut(s) 157, 384
Bse8I GATNNNNATC 3 cut(s) 105, 108, 111
BseDI CCNNGG 3 cut(s) 79, 649, 655
BseGI GGATG 1 cut(s) 691
BseJI GATNNNNATC 3 cut(s) 105, 108, 111
BseMI GCAATG 2 cut(s) 157, 384
BseMII CTCAG 2 cut(s) 632, 935
BseNI ACTGG 1 cut(s) 25
BseRI GAGGAG 2 cut(s) 111, 510
BseXI GCAGC 1 cut(s) 959
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 5 cut(s) 174, 315, 654, 660, 989
BsiSI CCGG 3 cut(s) 200, 251, 316
BslFI GGGAC 1 cut(s) 89
BsmAI GTCTC 1 cut(s) 201
BsmBI CGTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 5 cut(s) 174, 315, 654, 660, 989
Bsp119I TTCGAA 1 cut(s) 635
Bsp1407I TGTACA 1 cut(s) 188
Bsp143I GATC 5 cut(s) 68, 106, 375, 846, 882
Bsp19I CCATGG 3 cut(s) 79, 649, 655
BspACI CCGC 1 cut(s) 328
BspANI GGCC 5 cut(s) 174, 315, 654, 660, 989
BspCNI CTCAG 2 cut(s) 631, 934
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 2 cut(s) 77, 499
BspMI ACCTGC 1 cut(s) 266
BspPI GGATC 3 cut(s) 383, 841, 877
BspT104I TTCGAA 1 cut(s) 635
BsrDI GCAATG 2 cut(s) 157, 384
BsrGI TGTACA 1 cut(s) 188
BsrI ACTGG 1 cut(s) 25
BssECI CCNNGG 3 cut(s) 79, 649, 655
BssMI GATC 5 cut(s) 68, 106, 375, 846, 882
BssSI CACGAG 2 cut(s) 334, 698
BssT1I CCWWGG 3 cut(s) 79, 649, 655
Bst2BI CACGAG 2 cut(s) 334, 698
Bst4CI ACNGT 3 cut(s) 163, 188, 195
Bst6I CTCTTC 2 cut(s) 351, 978
BstAPI GCANNNNNTGC 2 cut(s) 532, 835
BstAUI TGTACA 1 cut(s) 188
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 3 cut(s) 313, 453, 550
BstDEI CTNAG 2 cut(s) 618, 921
BstDSI CCRYGG 3 cut(s) 79, 649, 655
BstEII GGTNACC 1 cut(s) 473
BstF5I GGATG 1 cut(s) 691
BstHHI GCGC 1 cut(s) 333
BstKTI GATC 5 cut(s) 71, 109, 378, 849, 885
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 5 cut(s) 68, 106, 375, 846, 882
BstMWI GCNNNNNNNGC 4 cut(s) 532, 826, 835, 876
BstPI GGTNACC 1 cut(s) 473
BstSFI CTRYAG 1 cut(s) 162
BstV1I GCAGC 1 cut(s) 959
BstX2I RGATCY 1 cut(s) 68
BstXI CCANNNNNNTGG 2 cut(s) 86, 656
BstYI RGATCY 1 cut(s) 68
BsuRI GGCC 5 cut(s) 174, 315, 654, 660, 989
BtgI CCRYGG 3 cut(s) 79, 649, 655
BtsCI GGATG 1 cut(s) 691
BveI ACCTGC 1 cut(s) 266
Cac8I GCNNGC 3 cut(s) 313, 453, 550
CciI TCATGA 1 cut(s) 414
CfoI GCGC 1 cut(s) 333
Cfr13I GGNCC 1 cut(s) 76
CseI GACGC 2 cut(s) 827, 889
Csp6I GTAC 5 cut(s) 184, 189, 434, 622, 670
CviAII CATG 9 cut(s) 80, 137, 415, 650, 656, 673, 764, 871, 880
CviQI GTAC 5 cut(s) 184, 189, 434, 622, 670
DdeI CTNAG 2 cut(s) 618, 921
DpnI GATC 5 cut(s) 70, 108, 377, 848, 884
DpnII GATC 5 cut(s) 68, 106, 375, 846, 882
DraI TTTAAA 1 cut(s) 810
DriI GACNNNNNGTC 1 cut(s) 427
EaeI YGGCCR 2 cut(s) 652, 658
Eam1104I CTCTTC 2 cut(s) 351, 978
Eam1105I GACNNNNNGTC 1 cut(s) 427
EarI CTCTTC 2 cut(s) 351, 978
Eco130I CCWWGG 3 cut(s) 79, 649, 655
Eco147I AGGCCT 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 76
Eco91I GGTNACC 1 cut(s) 473
EcoO65I GGTNACC 1 cut(s) 473
EcoT14I CCWWGG 3 cut(s) 79, 649, 655
ErhI CCWWGG 3 cut(s) 79, 649, 655
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 9 cut(s) 83, 140, 418, 653, 659, 676, 767, 874, 883
FalI AAGNNNNNCTT 2 cut(s) 55, 87
FaqI GGGAC 1 cut(s) 89
FatI CATG 9 cut(s) 79, 136, 414, 649, 655, 672, 763, 870, 879
FbaI TGATCA 1 cut(s) 106
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 2 cut(s) 329, 948
FokI GGATG 1 cut(s) 678
Fsp4HI GCNGC 2 cut(s) 329, 948
FspBI CTAG 3 cut(s) 39, 56, 294
GlaI GCGC 1 cut(s) 332
GluI GCNGC 2 cut(s) 329, 948
GsuI CTGGAG 1 cut(s) 583
HaeIII GGCC 5 cut(s) 174, 315, 654, 660, 989
HapII CCGG 3 cut(s) 200, 251, 316
HgaI GACGC 2 cut(s) 827, 889
HhaI GCGC 1 cut(s) 333
Hin1II CATG 9 cut(s) 83, 140, 418, 653, 659, 676, 767, 874, 883
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HinfI GANTC 3 cut(s) 11, 367, 976
HpaII CCGG 3 cut(s) 200, 251, 316
HphI GGTGA 5 cut(s) 217, 370, 485, 601, 845
Hpy166II GTNNAC 2 cut(s) 191, 220
Hpy188I TCNGA 5 cut(s) 31, 95, 372, 405, 574
Hpy188III TCNNGA 4 cut(s) 116, 346, 415, 980
Hpy8I GTNNAC 2 cut(s) 191, 220
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 5 cut(s) 116, 508, 622, 860, 966
HpyCH4III ACNGT 3 cut(s) 163, 188, 195
HpyCH4IV ACGT 1 cut(s) 861
HpyCH4V TGCA 6 cut(s) 261, 535, 691, 734, 829, 947
HpyF10VI GCNNNNNNNGC 4 cut(s) 532, 826, 835, 876
HpyF3I CTNAG 2 cut(s) 618, 921
HpySE526I ACGT 1 cut(s) 861
Hsp92II CATG 9 cut(s) 83, 140, 418, 653, 659, 676, 767, 874, 883
HspAI GCGC 1 cut(s) 331
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 5 cut(s) 68, 106, 375, 846, 882
LmnI GCTCC 2 cut(s) 497, 517
LpnPI CCDG 9 cut(s) 38, 213, 264, 271, 297, 329, 465, 562, 613
Lsp1109I GCAGC 1 cut(s) 959
LweI GCATC 3 cut(s) 438, 491, 700
MaeI CTAG 3 cut(s) 39, 56, 294
MaeII ACGT 1 cut(s) 861
MaeIII GTNAC 3 cut(s) 473, 745, 862
MalI GATC 5 cut(s) 70, 108, 377, 848, 884
MboI GATC 5 cut(s) 68, 106, 375, 846, 882
MboII GAAGA 5 cut(s) 131, 368, 567, 923, 995
MfeI CAATTG 1 cut(s) 729
MflI RGATCY 1 cut(s) 68
MlsI TGGCCA 2 cut(s) 654, 660
MluCI AATT 8 cut(s) 49, 229, 235, 241, 283, 505, 729, 916
MluNI TGGCCA 2 cut(s) 654, 660
MlyI GAGTC 1 cut(s) 376
Mox20I TGGCCA 2 cut(s) 654, 660
MscI TGGCCA 2 cut(s) 654, 660
MseI TTAA 2 cut(s) 809, 858
MslI CAYNNNNRTG 2 cut(s) 654, 776
Msp20I TGGCCA 2 cut(s) 654, 660
MspA1I CMGCKG 2 cut(s) 925, 950
MspI CCGG 3 cut(s) 200, 251, 316
MunI CAATTG 1 cut(s) 729
MwoI GCNNNNNNNGC 4 cut(s) 532, 826, 835, 876
NcoI CCATGG 3 cut(s) 79, 649, 655
NdeII GATC 5 cut(s) 68, 106, 375, 846, 882
NlaIII CATG 9 cut(s) 83, 140, 418, 653, 659, 676, 767, 874, 883
NlaIV GGNNCC 2 cut(s) 77, 499
NmuCI GTSAC 1 cut(s) 473
NspV TTCGAA 1 cut(s) 635
PagI TCATGA 1 cut(s) 414
PceI AGGCCT 1 cut(s) 174
PcsI WCGNNNNNNNCGW 1 cut(s) 632
PfeI GAWTC 2 cut(s) 11, 976
PkrI GCNGC 2 cut(s) 330, 949
PleI GAGTC 1 cut(s) 375
PpsI GAGTC 1 cut(s) 375
Psp1406I AACGTT 1 cut(s) 861
PspEI GGTNACC 1 cut(s) 473
PspN4I GGNNCC 2 cut(s) 77, 499
PspPI GGNCC 1 cut(s) 76
PsuI RGATCY 1 cut(s) 68
PvuII CAGCTG 2 cut(s) 925, 950
RsaI GTAC 5 cut(s) 185, 190, 435, 623, 671
RsaNI GTAC 5 cut(s) 184, 189, 434, 622, 670
RseI CAYNNNNRTG 2 cut(s) 654, 776
SaqAI TTAA 2 cut(s) 809, 858
SatI GCNGC 2 cut(s) 329, 948
Sau3AI GATC 5 cut(s) 68, 106, 375, 846, 882
Sau96I GGNCC 1 cut(s) 76
ScaI AGTACT 1 cut(s) 623
SchI GAGTC 1 cut(s) 376
SfaNI GCATC 3 cut(s) 438, 491, 700
SfcI CTRYAG 1 cut(s) 162
SfuI TTCGAA 1 cut(s) 635
SinI GGWCC 1 cut(s) 76
SmiMI CAYNNNNRTG 2 cut(s) 654, 776
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 8 cut(s) 49, 229, 235, 241, 283, 505, 729, 916
SseBI AGGCCT 1 cut(s) 174
SsiI CCGC 1 cut(s) 328
SspMI CTAG 3 cut(s) 39, 56, 294
StuI AGGCCT 1 cut(s) 174
StyI CCWWGG 3 cut(s) 79, 649, 655
TaaI ACNGT 3 cut(s) 163, 188, 195
TaiI ACGT 1 cut(s) 864
TaqI TCGA 2 cut(s) 626, 635
TasI AATT 8 cut(s) 49, 229, 235, 241, 283, 505, 729, 916
TatI WGTACW 3 cut(s) 188, 621, 669
TauI GCSGC 1 cut(s) 331
TfiI GAWTC 2 cut(s) 11, 976
Tru1I TTAA 2 cut(s) 809, 858
Tru9I TTAA 2 cut(s) 809, 858
TseFI GTSAC 1 cut(s) 473
TseI GCWGC 1 cut(s) 947
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 4 cut(s) 431, 689, 950, 975
TspGWI ACGGA 1 cut(s) 421
VpaK11BI GGWCC 1 cut(s) 76
XapI RAATTY 2 cut(s) 49, 505
XmiI GTMKAC 1 cut(s) 219
XspI CTAG 3 cut(s) 39, 56, 294
ZrmI AGTACT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.