pycom01g06400

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0015520
Physical Location & Seq
Reverse (-)
6665156 .. 6666215
1060 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g06400.1

Sequence Viewer

Length: 885 bp
ATGACATTAGAGGCGGTGGTGTTCCAAGAAGAACAATATAGCTATGGTTGGAAGGATTCGCAGGCCATAGGAGGAGGAGGTGGTGGTGGTGGATCTTGGAGCCATGGCTTTGATTTTGGAGAAGAAGATCACCAAGAAGAGAGTAATGAGGTAGGGCAACAAGGTTTCAATATGAGTTTGGATTCTTCACCTTCTTCAATGGTGCAAAGTGTCAATGACTGTGACACCAATAATGGGTCAGCTTTCAATCACGTGTTCCCTTCGATTGAAGCTCTGGCGGCCACCACAGGCCGGAAAAAGAGAAAGCGCATCCGAAGCATCAAGAACAAGGAAGTTATGGAGACACAAAGGATGACCCACATAGCTGTTGAACGCAATCGGAGGAAGCAAATGAATGTCTATCTTGCTGTCCTCCGCTCTATGATGCCCGCTCCCTATGCCCAAAGAGGAGACCAAGCATCAATTATAGGAGGAGCCATTAATTTCGTGAAGGAGCTTGAGCAACTTCTACAATCTATAGAAGGCCAGAGACGAACCAAGCAACAATCAGATCATCACTTAAATTTCGCCTCGATCTTCTCGAGCTTCTTTGTCTTCCCTCAATACTCGACCTGTTCGACCAACCATGGTCATGGCCTATACAACAATAGCTCAATAGAGCTTTCAAATGAGTTAACGGCTGAGAAGCGGTCGTCGGCGATTGCAGATGTTGAGGTGTCAATGGCGGAAAGCCATGCCAATATCAAAGTGCTTGTTAAAAAGCAGCCAAGGCAGCTCTTGAATATGGTGCTTGGTTTGCATTCTCTGCGCCTTATGATCCTTCACCTCAATGTCACTACCGTGGATAGTATGATCTTTTACTCTTTCAGTGTCAAGGTTAGTTAA

Protein Analysis

295

Amino Acids

32.77

Weight (kDa)

6.84

Isoelectric Point (pI)

62.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 116 - 167 6e-12 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015520)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20160 FvH4_1g20160
malus_domestica MD01G1037100.v1.1
prunus_persica Prupe.6G194400_v2.0.a1
pyrus_communis pycom01g06400
rosa_chinensis RchiOBHm_Chr2g0112221
rosa_laevigata RLG00000017960
rosa_multiflora Rmu_sc0001386.1_g000016 Rmu_sc0004467.1_g000007
rosa_roxburghii Rroxscaffold_2G00131650
rosa_rugosa Rorug02G0179300
rosa_samantha Rh2AG231500 Rh2BG244700 Rh2CG235900 Rh2DG239400
rosa_wichuraiana Rw2G017940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 417, 431
AciI CCGC 6 cut(s) 14, 278, 415, 429, 688, 725
AclWI GGATC 2 cut(s) 100, 811
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 1 cut(s) 562
AcvI CACGTG 1 cut(s) 253
AfiI CCNNNNNNNGG 2 cut(s) 234, 291
AflIII ACRYGT 1 cut(s) 252
AgsI TTSAA 7 cut(s) 169, 198, 247, 269, 371, 666, 781
AleI CACNNNNGTG 1 cut(s) 839
AluBI AGCT 9 cut(s) 42, 242, 272, 365, 496, 585, 651, 661, 775
AluI AGCT 9 cut(s) 42, 242, 272, 365, 496, 585, 651, 661, 775
Alw26I GTCTC 3 cut(s) 335, 444, 523
AlwI GGATC 2 cut(s) 100, 811
Ama87I CYCGRG 1 cut(s) 580
AoxI GGCC 5 cut(s) 63, 279, 289, 523, 634
ApeKI GCWGC 2 cut(s) 763, 772
ApoI RAATTY 1 cut(s) 562
AseI ATTAAT 1 cut(s) 480
AspLEI GCGC 2 cut(s) 309, 810
AsuHPI GGTGA 3 cut(s) 122, 180, 815
AvaI CYCGRG 1 cut(s) 580
BbrPI CACGTG 1 cut(s) 253
BbsI GAAGAC 1 cut(s) 586
BbvI GCAGC 2 cut(s) 775, 784
BceAI ACGGC 1 cut(s) 693
BcoDI GTCTC 3 cut(s) 335, 444, 523
BfmI CTRYAG 1 cut(s) 516
BisI GCNGC 3 cut(s) 279, 764, 773
BlsI GCNGC 3 cut(s) 280, 765, 774
BmeT110I CYCGRG 1 cut(s) 580
BmiI GGNNCC 2 cut(s) 101, 475
BmsI GCATC 4 cut(s) 318, 327, 414, 467
BpiI GAAGAC 1 cut(s) 586
BpuEI CTTGAG 1 cut(s) 518
BsaAI YACGTR 1 cut(s) 253
BsaI GGTCTC 1 cut(s) 444
BsaJI CCNNGG 4 cut(s) 103, 625, 767, 840
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 291
BseDI CCNNGG 4 cut(s) 103, 625, 767, 840
BseGI GGATG 2 cut(s) 309, 357
BseLI CCNNNNNNNGG 2 cut(s) 234, 291
BseMII CTCAG 1 cut(s) 672
BseRI GAGGAG 4 cut(s) 87, 90, 462, 486
BseXI GCAGC 2 cut(s) 775, 784
Bsh1285I CGRYCG 1 cut(s) 692
BshFI GGCC 5 cut(s) 65, 281, 291, 525, 636
BsiEI CGRYCG 1 cut(s) 692
BsiHKCI CYCGRG 1 cut(s) 580
BsiSI CCGG 1 cut(s) 292
BslI CCNNNNNNNGG 2 cut(s) 234, 291
BsmAI GTCTC 3 cut(s) 335, 444, 523
BsmBI CGTCTC 1 cut(s) 523
BsmI GAATGC 1 cut(s) 799
BsnI GGCC 5 cut(s) 65, 281, 291, 525, 636
Bso31I GGTCTC 1 cut(s) 444
BsoBI CYCGRG 1 cut(s) 580
Bsp143I GATC 6 cut(s) 92, 127, 550, 573, 816, 852
Bsp19I CCATGG 2 cut(s) 103, 625
BspACI CCGC 6 cut(s) 14, 278, 415, 429, 688, 725
BspANI GGCC 5 cut(s) 65, 281, 291, 525, 636
BspCNI CTCAG 1 cut(s) 673
BspLI GGNNCC 2 cut(s) 101, 475
BspPI GGATC 2 cut(s) 100, 811
BspTNI GGTCTC 1 cut(s) 444
BsrBI CCGCTC 2 cut(s) 417, 431
BssECI CCNNGG 4 cut(s) 103, 625, 767, 840
BssMI GATC 6 cut(s) 92, 127, 550, 573, 816, 852
BssT1I CCWWGG 3 cut(s) 103, 625, 767
Bst4CI ACNGT 2 cut(s) 221, 841
Bst6I CTCTTC 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 805
BstBAI YACGTR 1 cut(s) 253
BstC8I GCNNGC 2 cut(s) 63, 429
BstDEI CTNAG 1 cut(s) 681
BstDSI CCRYGG 3 cut(s) 103, 625, 840
BstF5I GGATG 2 cut(s) 309, 357
BstHHI GCGC 2 cut(s) 309, 810
BstKTI GATC 6 cut(s) 95, 130, 553, 576, 819, 855
BstMAI GTCTC 3 cut(s) 335, 444, 523
BstMBI GATC 6 cut(s) 92, 127, 550, 573, 816, 852
BstMCI CGRYCG 1 cut(s) 692
BstMWI GCNNNNNNNGC 7 cut(s) 278, 315, 437, 769, 772, 796, 805
BstSFI CTRYAG 1 cut(s) 516
BstV1I GCAGC 2 cut(s) 775, 784
BstV2I GAAGAC 1 cut(s) 586
BstX2I RGATCY 1 cut(s) 92
BstXI CCANNNNNNTGG 1 cut(s) 632
BstYI RGATCY 1 cut(s) 92
BsuRI GGCC 5 cut(s) 65, 281, 291, 525, 636
BtgI CCRYGG 3 cut(s) 103, 625, 840
BtsCI GGATG 2 cut(s) 309, 357
BtsIMutI CAGTG 1 cut(s) 874
Cac8I GCNNGC 2 cut(s) 63, 429
CfoI GCGC 2 cut(s) 309, 810
CviAII CATG 4 cut(s) 104, 626, 632, 734
DdeI CTNAG 1 cut(s) 681
DpnI GATC 6 cut(s) 94, 129, 552, 575, 818, 854
DpnII GATC 6 cut(s) 92, 127, 550, 573, 816, 852
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
EciI GGCGGA 1 cut(s) 740
Eco130I CCWWGG 3 cut(s) 103, 625, 767
Eco31I GGTCTC 1 cut(s) 444
Eco72I CACGTG 1 cut(s) 253
Eco88I CYCGRG 1 cut(s) 580
EcoT14I CCWWGG 3 cut(s) 103, 625, 767
ErhI CCWWGG 3 cut(s) 103, 625, 767
Esp3I CGTCTC 1 cut(s) 523
FaeI CATG 4 cut(s) 107, 629, 635, 737
FatI CATG 4 cut(s) 103, 625, 631, 733
FauI CCCGC 1 cut(s) 436
Fnu4HI GCNGC 3 cut(s) 279, 764, 773
FokI GGATG 2 cut(s) 296, 364
Fsp4HI GCNGC 3 cut(s) 279, 764, 773
GlaI GCGC 2 cut(s) 308, 809
GluI GCNGC 3 cut(s) 279, 764, 773
HaeIII GGCC 5 cut(s) 65, 281, 291, 525, 636
HapII CCGG 1 cut(s) 292
HhaI GCGC 2 cut(s) 309, 810
Hin1II CATG 4 cut(s) 107, 629, 635, 737
Hin6I GCGC 2 cut(s) 307, 808
HinP1I GCGC 2 cut(s) 307, 808
HincII GTYRAC 1 cut(s) 675
HindII GTYRAC 1 cut(s) 675
HinfI GANTC 2 cut(s) 56, 182
HpaI GTTAAC 1 cut(s) 675
HpaII CCGG 1 cut(s) 292
HphI GGTGA 3 cut(s) 122, 180, 815
Hpy166II GTNNAC 1 cut(s) 675
Hpy188I TCNGA 3 cut(s) 314, 381, 550
Hpy188III TCNNGA 4 cut(s) 322, 487, 580, 778
Hpy8I GTNNAC 1 cut(s) 675
Hpy99I CGWCG 1 cut(s) 697
HpyAV CCTTC 6 cut(s) 46, 201, 270, 484, 515, 830
HpyCH4III ACNGT 2 cut(s) 221, 841
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 3 cut(s) 205, 704, 799
HpyF10VI GCNNNNNNNGC 7 cut(s) 278, 315, 437, 769, 772, 796, 805
HpyF3I CTNAG 1 cut(s) 681
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 4 cut(s) 107, 629, 635, 737
HspAI GCGC 2 cut(s) 307, 808
KspAI GTTAAC 1 cut(s) 675
Kzo9I GATC 6 cut(s) 92, 127, 550, 573, 816, 852
LmnI GCTCC 4 cut(s) 99, 436, 473, 493
LpnPI CCDG 6 cut(s) 47, 260, 273, 305, 539, 625
Lsp1109I GCAGC 2 cut(s) 775, 784
LweI GCATC 4 cut(s) 318, 327, 414, 467
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 2 cut(s) 221, 832
MalI GATC 6 cut(s) 94, 129, 552, 575, 818, 854
MbiI CCGCTC 2 cut(s) 417, 431
MboI GATC 6 cut(s) 92, 127, 550, 573, 816, 852
MboII GAAGA 8 cut(s) 41, 134, 137, 149, 177, 186, 568, 586
MflI RGATCY 1 cut(s) 92
MluCI AATT 3 cut(s) 462, 481, 562
MmeI TCCRAC 1 cut(s) 29
MseI TTAA 5 cut(s) 480, 560, 674, 756, 883
MslI CAYNNNNRTG 3 cut(s) 630, 828, 839
MspI CCGG 1 cut(s) 292
Mva1269I GAATGC 1 cut(s) 799
MwoI GCNNNNNNNGC 7 cut(s) 278, 315, 437, 769, 772, 796, 805
NcoI CCATGG 2 cut(s) 103, 625
NdeII GATC 6 cut(s) 92, 127, 550, 573, 816, 852
NlaIII CATG 4 cut(s) 107, 629, 635, 737
NlaIV GGNNCC 2 cut(s) 101, 475
NmuCI GTSAC 2 cut(s) 221, 832
OliI CACNNNNGTG 1 cut(s) 839
PaeR7I CTCGAG 1 cut(s) 580
PcsI WCGNNNNNNNCGW 2 cut(s) 578, 614
PctI GAATGC 1 cut(s) 799
PfeI GAWTC 2 cut(s) 56, 182
PkrI GCNGC 3 cut(s) 280, 765, 774
PmaCI CACGTG 1 cut(s) 253
PmlI CACGTG 1 cut(s) 253
Ppu21I YACGTR 1 cut(s) 253
PshBI ATTAAT 1 cut(s) 480
PspCI CACGTG 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 101, 475
PsuI RGATCY 1 cut(s) 92
RseI CAYNNNNRTG 3 cut(s) 630, 828, 839
SaqAI TTAA 5 cut(s) 480, 560, 674, 756, 883
SatI GCNGC 3 cut(s) 279, 764, 773
Sau3AI GATC 6 cut(s) 92, 127, 550, 573, 816, 852
SfaNI GCATC 4 cut(s) 318, 327, 414, 467
SfcI CTRYAG 1 cut(s) 516
Sfr274I CTCGAG 1 cut(s) 580
SlaI CTCGAG 1 cut(s) 580
SmiMI CAYNNNNRTG 3 cut(s) 630, 828, 839
SmlI CTYRAG 2 cut(s) 497, 580
SmoI CTYRAG 2 cut(s) 497, 580
Sse9I AATT 3 cut(s) 462, 481, 562
SsiI CCGC 6 cut(s) 14, 278, 415, 429, 688, 725
StyI CCWWGG 3 cut(s) 103, 625, 767
TaaI ACNGT 2 cut(s) 221, 841
TaiI ACGT 1 cut(s) 255
TaqI TCGA 5 cut(s) 263, 572, 581, 608, 617
TasI AATT 3 cut(s) 462, 481, 562
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 2 cut(s) 56, 182
Tru1I TTAA 5 cut(s) 480, 560, 674, 756, 883
Tru9I TTAA 5 cut(s) 480, 560, 674, 756, 883
TscAI CASTG 1 cut(s) 874
TseFI GTSAC 2 cut(s) 221, 832
TseI GCWGC 2 cut(s) 763, 772
Tsp45I GTSAC 2 cut(s) 221, 832
TspDTI ATGAA 1 cut(s) 407
TspRI CASTG 1 cut(s) 874
VspI ATTAAT 1 cut(s) 480
XapI RAATTY 1 cut(s) 562
XhoI CTCGAG 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.