Rh2BG244700

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0015520
Physical Location & Seq
Forward (+)
24807050 .. 24808716
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG244700.1

Sequence Viewer

Length: 996 bp
ATGACGTTAGAAGCTGTGGTGTTCCAGCCAGACCCATTTAACTGTGGAAATTGTCTGGAAGGAAGTAGATCTTGGGACCATGGCTTTGGTTTCGGAGGAGATGATGATCAACAAGAAAAAGGCTACGCTCAACATGATATGCTCAACAATGGTAATATTGTAGGGCAAGGCCTTATGGGGTACTGTTCACCGTCACAGGTGAGAATGCAAAGTGTCAACACCGGCAACTCTAATTCCACGCCTGAAACCTGCACTGGCAACTTCAACTTCAACAATTCTTTCTCTAGCCCGATGGCCACCACAGGCCGGAGAAAGAGGCGGCGCACGAGAAACATCAAGAACAAGGAAGAGGTGGAGACTCAGAGGATCACCCACATTGCTGTTGAACGCAATCGGAGGAAGCTCATGAATGACTATCTGGCCGCATTGCGCTCTATGATGCCTGCTTCCTATGCCCAAAGGGGTGACCAAGCATCAATCATAGGAGGAGCCATAAATTTTGTGAAGGAGCTTGAGCAACTTCTGCAATCTTTAGAAGCCAGTAGAAGAACCAAACAATCACACTTGGATTTGGCCTCCATATTCTCCAACTTCTTTACCTTCCCTCAGTACTCGACCTGTTCGAGTACTAACACCCACGGCCATGGCCATAGCGAGTACATGAACAACAACTTGGTGCATCCCCACGAGATAATGGGGGAGAAGCGGTCAGGGGCAATTGCAGATGTGGAAGTAACAATGGTAGAAAGTCATGCGAACATTAAAGTGCTTTTGAAAAGACACCCAAGACAGCTCTTAAAGATGGTGGTTGGGTTGCACTCTCTGCGTCTTATGATCCTTCACCTTAATGTCACAACTATGGATAGCATGATCCTCTACTCTTTCAGCGTCAAGGTTGAAGATAATTCTCAGTTGACCTCAATGAATGATATTGCAGCTGATGTGTATGAAATGGTGGGAAGGATTCAAGAAGAGGCCCAGTTTTTACTTCAATAA

Protein Analysis

331

Amino Acids

37.25

Weight (kDa)

6.9

Isoelectric Point (pI)

53.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 121 - 172 3.1e-11 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015520)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20160 FvH4_1g20160
malus_domestica MD01G1037100.v1.1
prunus_persica Prupe.6G194400_v2.0.a1
pyrus_communis pycom01g06400
rosa_chinensis RchiOBHm_Chr2g0112221
rosa_laevigata RLG00000017960
rosa_multiflora Rmu_sc0001386.1_g000016 Rmu_sc0004467.1_g000007
rosa_roxburghii Rroxscaffold_2G00131650
rosa_rugosa Rorug02G0179300
rosa_samantha Rh2AG231500 Rh2BG244700 Rh2CG235900 Rh2DG239400
rosa_wichuraiana Rw2G017940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 257
AciI CCGC 3 cut(s) 319, 423, 706
AclWI GGATC 3 cut(s) 374, 829, 865
AcoI YGGCCR 4 cut(s) 294, 420, 640, 646
AcsI RAATTY 1 cut(s) 496
AfaI GTAC 4 cut(s) 182, 611, 628, 659
AfiI CCNNNNNNNGG 1 cut(s) 306
AgsI TTSAA 7 cut(s) 265, 271, 386, 775, 899, 968, 992
AjuI GAANNNNNNNTTGG 4 cut(s) 55, 87, 656, 688
AluBI AGCT 5 cut(s) 14, 403, 511, 793, 938
AluI AGCT 5 cut(s) 14, 403, 511, 793, 938
Alw26I GTCTC 1 cut(s) 350
AlwI GGATC 3 cut(s) 374, 829, 865
AoxI GGCC 8 cut(s) 169, 294, 304, 420, 573, 640, 646, 975
ApeKI GCWGC 1 cut(s) 935
ApoI RAATTY 1 cut(s) 496
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AspLEI GCGC 2 cut(s) 324, 432
AspS9I GGNCC 2 cut(s) 76, 976
AsuHPI GGTGA 5 cut(s) 180, 211, 361, 476, 833
AvaII GGWCC 1 cut(s) 76
BalI TGGCCA 2 cut(s) 296, 648
BauI CACGAG 2 cut(s) 325, 686
BbvI GCAGC 1 cut(s) 947
BccI CCATC 2 cut(s) 286, 796
BceAI ACGGC 1 cut(s) 655
BclI TGATCA 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 350
BfaI CTAG 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 257
BglII AGATCT 1 cut(s) 68
BisI GCNGC 3 cut(s) 320, 423, 936
BlsI GCNGC 3 cut(s) 321, 424, 937
BmcAI AGTACT 2 cut(s) 611, 628
Bme18I GGWCC 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 76, 976
BmiI GGNNCC 2 cut(s) 77, 490
BmrI ACTGGG 1 cut(s) 973
BmsI GCATC 3 cut(s) 429, 482, 688
BmuI ACTGGG 1 cut(s) 973
BpuEI CTTGAG 1 cut(s) 533
BsaBI GATNNNNATC 1 cut(s) 105
BsaJI CCNNGG 3 cut(s) 79, 637, 643
BsaXI ACNNNNNCTCC 2 cut(s) 692, 722
Bsc4I CCNNNNNNNGG 1 cut(s) 306
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 3 cut(s) 259, 540, 979
Bse3DI GCAATG 2 cut(s) 375, 425
Bse8I GATNNNNATC 1 cut(s) 105
BseDI CCNNGG 3 cut(s) 79, 637, 643
BseGI GGATG 1 cut(s) 679
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 1 cut(s) 306
BseMI GCAATG 2 cut(s) 375, 425
BseMII CTCAG 3 cut(s) 374, 620, 923
BseNI ACTGG 3 cut(s) 259, 540, 979
BseRI GAGGAG 2 cut(s) 111, 501
BseXI GCAGC 1 cut(s) 947
BsgI GTGCAG 1 cut(s) 235
BshFI GGCC 8 cut(s) 171, 296, 306, 422, 575, 642, 648, 977
BsiSI CCGG 2 cut(s) 222, 307
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 1 cut(s) 306
BsmAI GTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 89
BsmI GAATGC 1 cut(s) 210
BsnI GGCC 8 cut(s) 171, 296, 306, 422, 575, 642, 648, 977
Bsp143I GATC 5 cut(s) 68, 106, 366, 834, 870
Bsp19I CCATGG 2 cut(s) 79, 643
BspACI CCGC 3 cut(s) 319, 423, 706
BspANI GGCC 8 cut(s) 171, 296, 306, 422, 575, 642, 648, 977
BspCNI CTCAG 3 cut(s) 373, 619, 922
BspHI TCATGA 1 cut(s) 405
BspLI GGNNCC 2 cut(s) 77, 490
BspMI ACCTGC 1 cut(s) 257
BspPI GGATC 3 cut(s) 374, 829, 865
BsrDI GCAATG 2 cut(s) 375, 425
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 3 cut(s) 259, 540, 979
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 3 cut(s) 79, 637, 643
BssMI GATC 5 cut(s) 68, 106, 366, 834, 870
BssSI CACGAG 2 cut(s) 325, 686
BssT1I CCWWGG 2 cut(s) 79, 643
Bst2BI CACGAG 2 cut(s) 325, 686
Bst4CI ACNGT 3 cut(s) 44, 185, 192
Bst6I CTCTTC 2 cut(s) 342, 966
BstAPI GCANNNNNTGC 2 cut(s) 523, 823
BstC8I GCNNGC 1 cut(s) 444
BstDEI CTNAG 3 cut(s) 360, 606, 909
BstDSI CCRYGG 3 cut(s) 79, 637, 643
BstEII GGTNACC 1 cut(s) 464
BstF5I GGATG 1 cut(s) 679
BstHHI GCGC 2 cut(s) 324, 432
BstKTI GATC 5 cut(s) 71, 109, 369, 837, 873
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 5 cut(s) 68, 106, 366, 834, 870
BstMWI GCNNNNNNNGC 3 cut(s) 452, 523, 823
BstPI GGTNACC 1 cut(s) 464
BstV1I GCAGC 1 cut(s) 947
BstX2I RGATCY 1 cut(s) 68
BstXI CCANNNNNNTGG 2 cut(s) 86, 644
BstYI RGATCY 1 cut(s) 68
BsuRI GGCC 8 cut(s) 171, 296, 306, 422, 575, 642, 648, 977
BtgI CCRYGG 3 cut(s) 79, 637, 643
BtsCI GGATG 1 cut(s) 679
BtsIMutI CAGTG 1 cut(s) 252
BveI ACCTGC 1 cut(s) 257
Cac8I GCNNGC 1 cut(s) 444
CciI TCATGA 1 cut(s) 405
CfoI GCGC 2 cut(s) 324, 432
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 2 cut(s) 76, 976
CseI GACGC 2 cut(s) 815, 877
Csp6I GTAC 4 cut(s) 181, 610, 627, 658
CviAII CATG 7 cut(s) 80, 134, 406, 644, 661, 752, 868
CviQI GTAC 4 cut(s) 181, 610, 627, 658
DdeI CTNAG 3 cut(s) 360, 606, 909
DpnI GATC 5 cut(s) 70, 108, 368, 836, 872
DpnII GATC 5 cut(s) 68, 106, 366, 834, 870
EaeI YGGCCR 4 cut(s) 294, 420, 640, 646
Eam1104I CTCTTC 2 cut(s) 342, 966
EarI CTCTTC 2 cut(s) 342, 966
Eco130I CCWWGG 2 cut(s) 79, 643
Eco147I AGGCCT 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 76
Eco91I GGTNACC 1 cut(s) 464
EcoO65I GGTNACC 1 cut(s) 464
EcoT14I CCWWGG 2 cut(s) 79, 643
ErhI CCWWGG 2 cut(s) 79, 643
FaeI CATG 7 cut(s) 83, 137, 409, 647, 664, 755, 871
FalI AAGNNNNNCTT 2 cut(s) 55, 87
FaqI GGGAC 1 cut(s) 89
FatI CATG 7 cut(s) 79, 133, 405, 643, 660, 751, 867
FbaI TGATCA 1 cut(s) 106
Fnu4HI GCNGC 3 cut(s) 320, 423, 936
FokI GGATG 1 cut(s) 666
Fsp4HI GCNGC 3 cut(s) 320, 423, 936
FspBI CTAG 1 cut(s) 285
GlaI GCGC 2 cut(s) 323, 431
GluI GCNGC 3 cut(s) 320, 423, 936
HaeIII GGCC 8 cut(s) 171, 296, 306, 422, 575, 642, 648, 977
HapII CCGG 2 cut(s) 222, 307
HgaI GACGC 2 cut(s) 815, 877
HhaI GCGC 2 cut(s) 324, 432
Hin1II CATG 7 cut(s) 83, 137, 409, 647, 664, 755, 871
Hin6I GCGC 2 cut(s) 322, 430
HinP1I GCGC 2 cut(s) 322, 430
HincII GTYRAC 2 cut(s) 217, 915
HindII GTYRAC 2 cut(s) 217, 915
HinfI GANTC 2 cut(s) 358, 964
HpaII CCGG 2 cut(s) 222, 307
HphI GGTGA 5 cut(s) 180, 211, 361, 476, 833
Hpy166II GTNNAC 3 cut(s) 188, 217, 915
Hpy188I TCNGA 3 cut(s) 95, 363, 396
Hpy188III TCNNGA 4 cut(s) 56, 337, 406, 968
Hpy8I GTNNAC 3 cut(s) 188, 217, 915
HpyAV CCTTC 5 cut(s) 53, 499, 610, 848, 954
HpyCH4III ACNGT 3 cut(s) 44, 185, 192
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 7 cut(s) 208, 252, 526, 679, 722, 817, 935
HpyF10VI GCNNNNNNNGC 3 cut(s) 452, 523, 823
HpyF3I CTNAG 3 cut(s) 360, 606, 909
HpySE526I ACGT 1 cut(s) 5
Hsp92II CATG 7 cut(s) 83, 137, 409, 647, 664, 755, 871
HspAI GCGC 2 cut(s) 322, 430
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 5 cut(s) 68, 106, 366, 834, 870
LmnI GCTCC 2 cut(s) 488, 508
Lsp1109I GCAGC 1 cut(s) 947
LweI GCATC 3 cut(s) 429, 482, 688
MaeI CTAG 1 cut(s) 285
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 4 cut(s) 192, 464, 733, 850
MalI GATC 5 cut(s) 70, 108, 368, 836, 872
MboI GATC 5 cut(s) 68, 106, 366, 834, 870
MboII GAAGA 4 cut(s) 359, 558, 911, 983
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 1 cut(s) 68
MlsI TGGCCA 2 cut(s) 296, 648
MluCI AATT 6 cut(s) 49, 232, 274, 496, 717, 904
MluNI TGGCCA 2 cut(s) 296, 648
MlyI GAGTC 1 cut(s) 352
MmeI TCCRAC 1 cut(s) 612
Mox20I TGGCCA 2 cut(s) 296, 648
MscI TGGCCA 2 cut(s) 296, 648
MseI TTAA 4 cut(s) 39, 762, 797, 846
MslI CAYNNNNRTG 4 cut(s) 642, 764, 846, 857
Msp20I TGGCCA 2 cut(s) 296, 648
MspA1I CMGCKG 1 cut(s) 938
MspI CCGG 2 cut(s) 222, 307
MunI CAATTG 1 cut(s) 717
Mva1269I GAATGC 1 cut(s) 210
MwoI GCNNNNNNNGC 3 cut(s) 452, 523, 823
NcoI CCATGG 2 cut(s) 79, 643
NdeII GATC 5 cut(s) 68, 106, 366, 834, 870
NlaIII CATG 7 cut(s) 83, 137, 409, 647, 664, 755, 871
NlaIV GGNNCC 2 cut(s) 77, 490
NmuCI GTSAC 3 cut(s) 192, 464, 850
PagI TCATGA 1 cut(s) 405
PceI AGGCCT 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 620
PctI GAATGC 1 cut(s) 210
PfeI GAWTC 1 cut(s) 964
PkrI GCNGC 3 cut(s) 321, 424, 937
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
PspEI GGTNACC 1 cut(s) 464
PspN4I GGNNCC 2 cut(s) 77, 490
PspPI GGNCC 2 cut(s) 76, 976
PsuI RGATCY 1 cut(s) 68
PvuII CAGCTG 1 cut(s) 938
RsaI GTAC 4 cut(s) 182, 611, 628, 659
RsaNI GTAC 4 cut(s) 181, 610, 627, 658
RseI CAYNNNNRTG 4 cut(s) 642, 764, 846, 857
SaqAI TTAA 4 cut(s) 39, 762, 797, 846
SatI GCNGC 3 cut(s) 320, 423, 936
Sau3AI GATC 5 cut(s) 68, 106, 366, 834, 870
Sau96I GGNCC 2 cut(s) 76, 976
ScaI AGTACT 2 cut(s) 611, 628
SchI GAGTC 1 cut(s) 352
SfaNI GCATC 3 cut(s) 429, 482, 688
SinI GGWCC 1 cut(s) 76
SmiMI CAYNNNNRTG 4 cut(s) 642, 764, 846, 857
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 6 cut(s) 49, 232, 274, 496, 717, 904
SseBI AGGCCT 1 cut(s) 171
SsiI CCGC 3 cut(s) 319, 423, 706
SspI AATATT 1 cut(s) 157
SspMI CTAG 1 cut(s) 285
StuI AGGCCT 1 cut(s) 171
StyI CCWWGG 2 cut(s) 79, 643
TaaI ACNGT 3 cut(s) 44, 185, 192
TaiI ACGT 1 cut(s) 8
TaqI TCGA 2 cut(s) 614, 623
TasI AATT 6 cut(s) 49, 232, 274, 496, 717, 904
TatI WGTACW 3 cut(s) 609, 626, 657
TauI GCSGC 2 cut(s) 322, 425
TfiI GAWTC 1 cut(s) 964
Tru1I TTAA 4 cut(s) 39, 762, 797, 846
Tru9I TTAA 4 cut(s) 39, 762, 797, 846
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 3 cut(s) 192, 464, 850
TseI GCWGC 1 cut(s) 935
Tsp45I GTSAC 3 cut(s) 192, 464, 850
TspDTI ATGAA 4 cut(s) 422, 677, 938, 963
TspRI CASTG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 76
XapI RAATTY 1 cut(s) 496
XspI CTAG 1 cut(s) 285
ZrmI AGTACT 2 cut(s) 611, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.