MD01G1037100.v1.1

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0015520
Physical Location & Seq
Reverse (-)
12423492 .. 12425620
2129 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1037100.v1.1.491

Sequence Viewer

Length: 978 bp
ATGACATTAGAGGCGGTGGTGTTCCAAGAAGAACAATATAGCCATGGTTGGAAGGATTCGCAGGCCATAGGAGGAGGTGGTGGTGGTGGTGGATCTTGGAGCCATGGCTTTGATTTTGGAGAAGAAGATCACCAAGAAGAGAGTAATAAGGTAGGGCAACAAGGTTTCAATATGAGTTTGGACTCTTCACCTTCTTCAATGGTGCAAAGTGTCAATGACTTTGACAAAAATAATGGGTCAGCTTTCAATCACGTTTTCCCTTCGATTGAAGCTCTGGCAGCCACCACAGGCCGGAAGAAGAGAAAGCGCATCCGAAGCATCAAGAACAAGGAATTTATGGAGACACAAAGGATGACCCACATTGCTGTTGAACGCAATCGGAGGAAGCAAATGAATGTCTATCTTGCTGCCCTCCGCTCTATGATGCCCGCTCCCTATGCCCAAAGAGGAGACCAAGCATCAATTATCGGAGGAGCCATTAATTTCGTGAAGGAGCTTGAGCAACTTCTACAATCTATAGAAGGCCAGAGACGAACCAATCAACAATCAGATCATCACTTAAATTTCGCCTCGATCTTCTCTAGCTTCTTCGCCTTCCCTCAGTACTCGAACTGTTCGACCAACCATGGCCATGGCCTATACAACAATAGCTCAATAGAGCTTTCAAATGAGTTAACGGCTGAGAAGCGGTCGTCGGCGATTGCAGATGTTGAAGTGTCAATGGCGGAAAGCCATGCCAATATCAAAGTGCTTGTTAAAAAGCAGCCAAGGCAGCTCTTGAATATGGTGCTTGGTTTGCATTCTCTGCGCTTTATGATCCTTCACCTCAATGTCACTACCGTGGATAGTATGATCCTTTACTCTTTCAGTGTCAAGATTGAAGATAATTCTCAGCTGACCTCAGTGAATGAGATTGCAGCTGATGTGTATGAAATGGTGGGTAGGATTCAGGAGGAAGCTCAGCATTTAGCTCAATAA

Protein Analysis

326

Amino Acids

36.31

Weight (kDa)

6.23

Isoelectric Point (pI)

60.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 116 - 167 2.8e-12 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015520)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20160 FvH4_1g20160
malus_domestica MD01G1037100.v1.1
prunus_persica Prupe.6G194400_v2.0.a1
pyrus_communis pycom01g06400
rosa_chinensis RchiOBHm_Chr2g0112221
rosa_laevigata RLG00000017960
rosa_multiflora Rmu_sc0001386.1_g000016 Rmu_sc0004467.1_g000007
rosa_roxburghii Rroxscaffold_2G00131650
rosa_rugosa Rorug02G0179300
rosa_samantha Rh2AG231500 Rh2BG244700 Rh2CG235900 Rh2DG239400
rosa_wichuraiana Rw2G017940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 417, 431
AciI CCGC 5 cut(s) 14, 415, 429, 688, 725
AclWI GGATC 3 cut(s) 100, 811, 847
AcoI YGGCCR 1 cut(s) 628
AcsI RAATTY 2 cut(s) 332, 562
AfaI GTAC 1 cut(s) 605
AfiI CCNNNNNNNGG 1 cut(s) 291
AgsI TTSAA 9 cut(s) 169, 198, 247, 269, 371, 666, 713, 781, 881
AleI CACNNNNGTG 1 cut(s) 839
Alw26I GTCTC 3 cut(s) 335, 444, 523
AlwI GGATC 3 cut(s) 100, 811, 847
AoxI GGCC 5 cut(s) 63, 289, 523, 628, 634
ApeKI GCWGC 5 cut(s) 278, 407, 763, 772, 917
ApoI RAATTY 2 cut(s) 332, 562
AseI ATTAAT 1 cut(s) 480
AspLEI GCGC 2 cut(s) 309, 810
AsuHPI GGTGA 3 cut(s) 122, 180, 815
BalI TGGCCA 1 cut(s) 630
BbvI GCAGC 5 cut(s) 290, 394, 775, 784, 929
BceAI ACGGC 1 cut(s) 693
BcoDI GTCTC 3 cut(s) 335, 444, 523
BfaI CTAG 1 cut(s) 582
BfmI CTRYAG 1 cut(s) 516
BisI GCNGC 5 cut(s) 279, 408, 764, 773, 918
BlpI GCTNAGC 1 cut(s) 960
BlsI GCNGC 5 cut(s) 280, 409, 765, 774, 919
BmcAI AGTACT 1 cut(s) 605
BmiI GGNNCC 2 cut(s) 101, 475
BmsI GCATC 4 cut(s) 318, 327, 414, 467
Bpu1102I GCTNAGC 1 cut(s) 960
BpuEI CTTGAG 1 cut(s) 518
BsaI GGTCTC 1 cut(s) 444
BsaJI CCNNGG 6 cut(s) 43, 103, 625, 631, 767, 840
Bsc4I CCNNNNNNNGG 1 cut(s) 291
Bse3DI GCAATG 1 cut(s) 360
BseDI CCNNGG 6 cut(s) 43, 103, 625, 631, 767, 840
BseGI GGATG 2 cut(s) 309, 357
BseLI CCNNNNNNNGG 1 cut(s) 291
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 5 cut(s) 614, 672, 905, 915, 974
BseRI GAGGAG 3 cut(s) 87, 462, 486
BseXI GCAGC 5 cut(s) 290, 394, 775, 784, 929
Bsh1285I CGRYCG 1 cut(s) 692
BshFI GGCC 5 cut(s) 65, 291, 525, 630, 636
BsiEI CGRYCG 1 cut(s) 692
BsiSI CCGG 1 cut(s) 292
BslI CCNNNNNNNGG 1 cut(s) 291
BsmAI GTCTC 3 cut(s) 335, 444, 523
BsmBI CGTCTC 1 cut(s) 523
BsmI GAATGC 1 cut(s) 799
BsnI GGCC 5 cut(s) 65, 291, 525, 630, 636
Bso31I GGTCTC 1 cut(s) 444
Bsp143I GATC 6 cut(s) 92, 127, 550, 573, 816, 852
Bsp1720I GCTNAGC 1 cut(s) 960
Bsp19I CCATGG 4 cut(s) 43, 103, 625, 631
BspACI CCGC 5 cut(s) 14, 415, 429, 688, 725
BspANI GGCC 5 cut(s) 65, 291, 525, 630, 636
BspCNI CTCAG 5 cut(s) 613, 673, 904, 914, 973
BspLI GGNNCC 2 cut(s) 101, 475
BspPI GGATC 3 cut(s) 100, 811, 847
BspTNI GGTCTC 1 cut(s) 444
BsrBI CCGCTC 2 cut(s) 417, 431
BsrDI GCAATG 1 cut(s) 360
BssECI CCNNGG 6 cut(s) 43, 103, 625, 631, 767, 840
BssMI GATC 6 cut(s) 92, 127, 550, 573, 816, 852
BssT1I CCWWGG 5 cut(s) 43, 103, 625, 631, 767
Bst4CI ACNGT 2 cut(s) 614, 841
Bst6I CTCTTC 3 cut(s) 132, 190, 293
BstAPI GCANNNNNTGC 1 cut(s) 805
BstC8I GCNNGC 2 cut(s) 63, 429
BstDEI CTNAG 5 cut(s) 600, 681, 891, 901, 960
BstDSI CCRYGG 5 cut(s) 43, 103, 625, 631, 840
BstF5I GGATG 2 cut(s) 309, 357
BstHHI GCGC 2 cut(s) 309, 810
BstKTI GATC 6 cut(s) 95, 130, 553, 576, 819, 855
BstMAI GTCTC 3 cut(s) 335, 444, 523
BstMBI GATC 6 cut(s) 92, 127, 550, 573, 816, 852
BstMCI CGRYCG 1 cut(s) 692
BstMWI GCNNNNNNNGC 7 cut(s) 278, 315, 437, 769, 772, 796, 805
BstSFI CTRYAG 1 cut(s) 516
BstV1I GCAGC 5 cut(s) 290, 394, 775, 784, 929
BstX2I RGATCY 1 cut(s) 92
BstXI CCANNNNNNTGG 1 cut(s) 632
BstYI RGATCY 1 cut(s) 92
BsuRI GGCC 5 cut(s) 65, 291, 525, 630, 636
BtgI CCRYGG 5 cut(s) 43, 103, 625, 631, 840
BtsCI GGATG 2 cut(s) 309, 357
BtsIMutI CAGTG 2 cut(s) 874, 909
Cac8I GCNNGC 2 cut(s) 63, 429
CfoI GCGC 2 cut(s) 309, 810
Csp6I GTAC 1 cut(s) 604
CviAII CATG 5 cut(s) 44, 104, 626, 632, 734
CviQI GTAC 1 cut(s) 604
DdeI CTNAG 5 cut(s) 600, 681, 891, 901, 960
DpnI GATC 6 cut(s) 94, 129, 552, 575, 818, 854
DpnII GATC 6 cut(s) 92, 127, 550, 573, 816, 852
EaeI YGGCCR 1 cut(s) 628
Eam1104I CTCTTC 3 cut(s) 132, 190, 293
EarI CTCTTC 3 cut(s) 132, 190, 293
EciI GGCGGA 1 cut(s) 740
Eco130I CCWWGG 5 cut(s) 43, 103, 625, 631, 767
Eco31I GGTCTC 1 cut(s) 444
EcoT14I CCWWGG 5 cut(s) 43, 103, 625, 631, 767
ErhI CCWWGG 5 cut(s) 43, 103, 625, 631, 767
Esp3I CGTCTC 1 cut(s) 523
FaeI CATG 5 cut(s) 47, 107, 629, 635, 737
FatI CATG 5 cut(s) 43, 103, 625, 631, 733
FauI CCCGC 1 cut(s) 436
Fnu4HI GCNGC 5 cut(s) 279, 408, 764, 773, 918
FokI GGATG 2 cut(s) 296, 364
Fsp4HI GCNGC 5 cut(s) 279, 408, 764, 773, 918
FspBI CTAG 1 cut(s) 582
GlaI GCGC 2 cut(s) 308, 809
GluI GCNGC 5 cut(s) 279, 408, 764, 773, 918
HaeIII GGCC 5 cut(s) 65, 291, 525, 630, 636
HapII CCGG 1 cut(s) 292
HhaI GCGC 2 cut(s) 309, 810
Hin1II CATG 5 cut(s) 47, 107, 629, 635, 737
Hin6I GCGC 2 cut(s) 307, 808
HinP1I GCGC 2 cut(s) 307, 808
HincII GTYRAC 1 cut(s) 675
HindII GTYRAC 1 cut(s) 675
HinfI GANTC 3 cut(s) 56, 182, 946
HpaI GTTAAC 1 cut(s) 675
HpaII CCGG 1 cut(s) 292
HphI GGTGA 3 cut(s) 122, 180, 815
Hpy166II GTNNAC 1 cut(s) 675
Hpy188I TCNGA 4 cut(s) 314, 381, 470, 550
Hpy188III TCNNGA 5 cut(s) 322, 487, 778, 874, 950
Hpy8I GTNNAC 1 cut(s) 675
Hpy99I CGWCG 1 cut(s) 697
HpyAV CCTTC 7 cut(s) 46, 201, 270, 484, 515, 604, 830
HpyCH4III ACNGT 2 cut(s) 614, 841
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 4 cut(s) 205, 704, 799, 917
HpyF10VI GCNNNNNNNGC 7 cut(s) 278, 315, 437, 769, 772, 796, 805
HpyF3I CTNAG 5 cut(s) 600, 681, 891, 901, 960
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 5 cut(s) 47, 107, 629, 635, 737
HspAI GCGC 2 cut(s) 307, 808
KspAI GTTAAC 1 cut(s) 675
Kzo9I GATC 6 cut(s) 92, 127, 550, 573, 816, 852
LmnI GCTCC 4 cut(s) 99, 436, 473, 493
LpnPI CCDG 6 cut(s) 47, 260, 273, 305, 539, 935
Lsp1109I GCAGC 5 cut(s) 290, 394, 775, 784, 929
LweI GCATC 4 cut(s) 318, 327, 414, 467
MaeI CTAG 1 cut(s) 582
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 1 cut(s) 832
MalI GATC 6 cut(s) 94, 129, 552, 575, 818, 854
MbiI CCGCTC 2 cut(s) 417, 431
MboI GATC 6 cut(s) 92, 127, 550, 573, 816, 852
MflI RGATCY 1 cut(s) 92
MlsI TGGCCA 1 cut(s) 630
MluCI AATT 5 cut(s) 332, 462, 481, 562, 886
MluNI TGGCCA 1 cut(s) 630
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 29
Mox20I TGGCCA 1 cut(s) 630
MscI TGGCCA 1 cut(s) 630
MseI TTAA 4 cut(s) 480, 560, 674, 756
MslI CAYNNNNRTG 3 cut(s) 630, 828, 839
Msp20I TGGCCA 1 cut(s) 630
MspA1I CMGCKG 2 cut(s) 895, 920
MspI CCGG 1 cut(s) 292
Mva1269I GAATGC 1 cut(s) 799
MwoI GCNNNNNNNGC 7 cut(s) 278, 315, 437, 769, 772, 796, 805
NcoI CCATGG 4 cut(s) 43, 103, 625, 631
NdeII GATC 6 cut(s) 92, 127, 550, 573, 816, 852
NlaIII CATG 5 cut(s) 47, 107, 629, 635, 737
NlaIV GGNNCC 2 cut(s) 101, 475
NmuCI GTSAC 1 cut(s) 832
OliI CACNNNNGTG 1 cut(s) 839
PcsI WCGNNNNNNNCGW 1 cut(s) 614
PctI GAATGC 1 cut(s) 799
PfeI GAWTC 2 cut(s) 56, 946
PkrI GCNGC 5 cut(s) 280, 409, 765, 774, 919
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
PshBI ATTAAT 1 cut(s) 480
PspN4I GGNNCC 2 cut(s) 101, 475
PsuI RGATCY 1 cut(s) 92
PvuII CAGCTG 2 cut(s) 895, 920
RsaI GTAC 1 cut(s) 605
RsaNI GTAC 1 cut(s) 604
RseI CAYNNNNRTG 3 cut(s) 630, 828, 839
SaqAI TTAA 4 cut(s) 480, 560, 674, 756
SatI GCNGC 5 cut(s) 279, 408, 764, 773, 918
Sau3AI GATC 6 cut(s) 92, 127, 550, 573, 816, 852
ScaI AGTACT 1 cut(s) 605
SchI GAGTC 1 cut(s) 176
SfaNI GCATC 4 cut(s) 318, 327, 414, 467
SfcI CTRYAG 1 cut(s) 516
SmiMI CAYNNNNRTG 3 cut(s) 630, 828, 839
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
Sse9I AATT 5 cut(s) 332, 462, 481, 562, 886
SsiI CCGC 5 cut(s) 14, 415, 429, 688, 725
SspMI CTAG 1 cut(s) 582
StyI CCWWGG 5 cut(s) 43, 103, 625, 631, 767
TaaI ACNGT 2 cut(s) 614, 841
TaiI ACGT 1 cut(s) 255
TaqI TCGA 4 cut(s) 263, 572, 608, 617
TasI AATT 5 cut(s) 332, 462, 481, 562, 886
TatI WGTACW 1 cut(s) 603
TfiI GAWTC 2 cut(s) 56, 946
Tru1I TTAA 4 cut(s) 480, 560, 674, 756
Tru9I TTAA 4 cut(s) 480, 560, 674, 756
TscAI CASTG 2 cut(s) 874, 909
TseFI GTSAC 1 cut(s) 832
TseI GCWGC 5 cut(s) 278, 407, 763, 772, 917
Tsp45I GTSAC 1 cut(s) 832
TspDTI ATGAA 2 cut(s) 407, 945
TspRI CASTG 2 cut(s) 874, 909
VspI ATTAAT 1 cut(s) 480
XapI RAATTY 2 cut(s) 332, 562
XspI CTAG 1 cut(s) 582
ZrmI AGTACT 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.