FvH4_1g21640

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014574
Physical Location & Seq
Reverse (-)
13612842 .. 13613941
1100 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g21640.t1

Sequence Viewer

Length: 459 bp
ATGGGAGTTGGAGGAACTTTGGAGTACTTGTCTGATCTAATGGGTAGTGGTGGCCATAAACACAAGAAGAAGAAGCAGTTACAGACTGTGGAGCTCAAAGTCAGAATGGACTGTGATGGTTGTGAGCTTAAGGTCAAGAAGGCACTCTCTTCTTTAAGCGGAGTTAAATCTGTGGAGATTAACAGAAAGCAACAGAAGGCGAGTGTTACTGGGTATGTTGAACCCAACAAGGTGCTGAAGAAGGCAAAGTCTACAGGGAAGAGGGCAGAGATATGGCCTTATGTGCCTTACAATCTAGTGGCTCAGCCTTACATGGCTCCAGCTTATGACAAGAAGGCACCTCCTGGTTATGTCAGGAAAGTGGAGAACACTGCAACTACTGGCACCATGACAAGATATGAAGACCCCTACACAGCCATGTTCAGTGATGAAAACCCCAATGCCTGCTCTATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

153

Amino Acids

16.83

Weight (kDa)

9.48

Isoelectric Point (pI)

35.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 33 - 87 3.3e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014574)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39700
fragaria_vesca FvH4_1g21640
malus_domestica MD01G1024200.v1.1 MD15G1328000.v1.1
prunus_persica Prupe.6G179300_v2.0.a1
pyrus_communis pycom01g05190 pycom15g29660
rosa_chinensis RchiOBHm_Chr2g0115511
rosa_laevigata RLG00000018189
rosa_multiflora Rmu_sc0008334.1_g000016
rosa_roxburghii Rroxscaffold_2G00129020
rosa_rugosa Rorug02G0197200
rosa_samantha Rh2AG254800 Rh2BG265500 Rh2CG260000 Rh2DG262900
rosa_wichuraiana Rw2G019940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 337, 383
AccI GTMKAC 1 cut(s) 251
AciI CCGC 1 cut(s) 159
AcoI YGGCCR 1 cut(s) 52
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 1 cut(s) 26
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 1 cut(s) 221
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 3 cut(s) 94, 127, 323
AluI AGCT 3 cut(s) 94, 127, 323
Alw21I GWGCWC 1 cut(s) 96
AoxI GGCC 2 cut(s) 52, 275
BalI TGGCCA 1 cut(s) 54
BanI GGYRCC 2 cut(s) 337, 383
BanII GRGCYC 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 96
BccI CCATC 1 cut(s) 110
BciT130I CCWGG 1 cut(s) 345
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 252
BfrI CTTAAG 1 cut(s) 128
BlpI GCTNAGC 1 cut(s) 303
BmcAI AGTACT 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 345
BmiI GGNNCC 3 cut(s) 318, 339, 385
BmrFI CCNGG 1 cut(s) 345
BmrI ACTGGG 1 cut(s) 219
BmuI ACTGGG 1 cut(s) 219
BpiI GAAGAC 1 cut(s) 408
BpmI CTGGAG 1 cut(s) 303
Bpu1102I GCTNAGC 1 cut(s) 303
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bse1I ACTGG 2 cut(s) 214, 385
BseBI CCWGG 1 cut(s) 345
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 2 cut(s) 214, 385
BshFI GGCC 2 cut(s) 54, 277
BshNI GGYRCC 2 cut(s) 337, 383
BsiHKAI GWGCWC 1 cut(s) 96
BsnI GGCC 2 cut(s) 54, 277
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 1 cut(s) 34
Bsp1720I GCTNAGC 1 cut(s) 303
BspACI CCGC 1 cut(s) 159
BspANI GGCC 2 cut(s) 54, 277
BspCNI CTCAG 1 cut(s) 316
BspLI GGNNCC 3 cut(s) 318, 339, 385
BspT107I GGYRCC 2 cut(s) 337, 383
BspTI CTTAAG 1 cut(s) 128
BsrI ACTGG 2 cut(s) 214, 385
BssMI GATC 1 cut(s) 34
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 88, 113
Bst6I CTCTTC 2 cut(s) 154, 254
BstAFI CTTAAG 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 445
BstDEI CTNAG 1 cut(s) 303
BstKTI GATC 1 cut(s) 37
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 1 cut(s) 283
BstNI CCWGG 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 343
BstSFI CTRYAG 1 cut(s) 252
BstV2I GAAGAC 1 cut(s) 408
BsuRI GGCC 2 cut(s) 54, 277
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 2 cut(s) 369, 430
Cac8I GCNNGC 1 cut(s) 445
Csp6I GTAC 1 cut(s) 25
CviAII CATG 4 cut(s) 313, 388, 418, 454
CviJI RGCY 9 cut(s) 54, 94, 127, 277, 302, 307, 317, 323, 416
CviKI_1 RGCY 9 cut(s) 54, 94, 127, 277, 302, 307, 317, 323, 416
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 1 cut(s) 303
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
EaeI YGGCCR 1 cut(s) 52
Eam1104I CTCTTC 2 cut(s) 154, 254
EarI CTCTTC 2 cut(s) 154, 254
Ecl136II GAGCTC 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 96
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 96
FaeI CATG 4 cut(s) 316, 391, 421, 457
FatI CATG 4 cut(s) 312, 387, 417, 453
FblI GTMKAC 1 cut(s) 251
FriOI GRGCYC 1 cut(s) 96
FspBI CTAG 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 2 cut(s) 54, 277
Hin1II CATG 4 cut(s) 316, 391, 421, 457
Hpy166II GTNNAC 1 cut(s) 252
Hpy188I TCNGA 2 cut(s) 34, 104
Hpy188III TCNNGA 2 cut(s) 136, 355
Hpy8I GTNNAC 1 cut(s) 252
HpyAV CCTTC 4 cut(s) 133, 190, 235, 328
HpyCH4III ACNGT 2 cut(s) 88, 113
HpyCH4V TGCA 1 cut(s) 374
HpyF10VI GCNNNNNNNGC 1 cut(s) 283
HpyF3I CTNAG 1 cut(s) 303
Hsp92II CATG 4 cut(s) 316, 391, 421, 457
Kzo9I GATC 1 cut(s) 34
LmnI GCTCC 2 cut(s) 91, 322
LpnPI CCDG 7 cut(s) 195, 240, 330, 333, 340, 357, 366
MaeI CTAG 1 cut(s) 296
MaeIII GTNAC 2 cut(s) 78, 205
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MboII GAAGA 6 cut(s) 79, 82, 141, 250, 271, 413
MhlI GDGCHC 1 cut(s) 96
MlsI TGGCCA 1 cut(s) 54
MluNI TGGCCA 1 cut(s) 54
MnlI CCTC 3 cut(s) 5, 255, 351
Mox20I TGGCCA 1 cut(s) 54
MscI TGGCCA 1 cut(s) 54
MseI TTAA 4 cut(s) 129, 155, 165, 180
MslI CAYNNNNRTG 1 cut(s) 416
Msp20I TGGCCA 1 cut(s) 54
MspCI CTTAAG 1 cut(s) 128
MspR9I CCNGG 1 cut(s) 345
MvaI CCWGG 1 cut(s) 345
MwoI GCNNNNNNNGC 1 cut(s) 283
NdeII GATC 1 cut(s) 34
NlaIII CATG 4 cut(s) 316, 391, 421, 457
NlaIV GGNNCC 3 cut(s) 318, 339, 385
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 3 cut(s) 318, 339, 385
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
RseI CAYNNNNRTG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 4 cut(s) 129, 155, 165, 180
Sau3AI GATC 1 cut(s) 34
ScaI AGTACT 1 cut(s) 26
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 1 cut(s) 96
SetI ASST 6 cut(s) 96, 129, 135, 234, 325, 343
SfcI CTRYAG 1 cut(s) 252
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
SsiI CCGC 1 cut(s) 159
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 2 cut(s) 88, 113
TatI WGTACW 1 cut(s) 24
Tru1I TTAA 4 cut(s) 129, 155, 165, 180
Tru9I TTAA 4 cut(s) 129, 155, 165, 180
TscAI CASTG 2 cut(s) 376, 430
TspDTI ATGAA 2 cut(s) 414, 444
TspRI CASTG 2 cut(s) 376, 430
Vha464I CTTAAG 1 cut(s) 128
XmiI GTMKAC 1 cut(s) 251
XspI CTAG 1 cut(s) 296
ZrmI AGTACT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.