RchiOBHm_Chr2g0115511

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014574
Physical Location & Seq
Reverse (-)
27638166 .. 27639411
1246 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48867

Sequence Viewer

Length: 459 bp
ATGGGAGTTGGAGGAACTTTGGAGTACTTGTCTGATCTAATGGGTAGTGCTGGCCATAAACACAAAAGGAAGAAGCAATTACAGACTGTGGAGCTCAAAGTCAGAATGGACTGTGATGGTTGTGAGCTTAAGGTCAAGAAGACCCTTTCTTCTTTAAGTGGAGTTAAATCTGTGGAGATTAACAGAAAGCAACAGAAGGTGAGTGTTACAGGGTATGTTGAACCCAACAAGGTGCTGAAGAAGGCAAAGTCAACAGGGAAAAGGGCAGAGATATGGCCTTATGTTCCCTACAATCTAGTGGCTCAGCCCTACATGGCTCAAGCTTATGACAAGAAGGCACCTCCTGGTTATGTCAGGAAAGTGGAGAACACTGCAACTACTGGCACCATGATAAGATATGAAGATCCCTACACCGCCATGTTCAGTGATGAAAACCCCAACGCCTGCTCTATCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

152

Amino Acids

16.97

Weight (kDa)

9.5

Isoelectric Point (pI)

33.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 33 - 87 4.1e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014574)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39700
fragaria_vesca FvH4_1g21640
malus_domestica MD01G1024200.v1.1 MD15G1328000.v1.1
prunus_persica Prupe.6G179300_v2.0.a1
pyrus_communis pycom01g05190 pycom15g29660
rosa_chinensis RchiOBHm_Chr2g0115511
rosa_laevigata RLG00000018189
rosa_multiflora Rmu_sc0008334.1_g000016
rosa_roxburghii Rroxscaffold_2G00129020
rosa_rugosa Rorug02G0197200
rosa_samantha Rh2AG254800 Rh2BG265500 Rh2CG260000 Rh2DG262900
rosa_wichuraiana Rw2G019940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 337, 383
AciI CCGC 1 cut(s) 414
AclWI GGATC 1 cut(s) 398
AcoI YGGCCR 1 cut(s) 52
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 1 cut(s) 26
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 1 cut(s) 221
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 3 cut(s) 94, 127, 323
AluI AGCT 3 cut(s) 94, 127, 323
Alw21I GWGCWC 1 cut(s) 96
AlwI GGATC 1 cut(s) 398
AoxI GGCC 2 cut(s) 52, 275
AsuHPI GGTGA 1 cut(s) 211
BalI TGGCCA 1 cut(s) 54
BanI GGYRCC 2 cut(s) 337, 383
BanII GRGCYC 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 96
BccI CCATC 1 cut(s) 110
BciT130I CCWGG 1 cut(s) 345
BfaI CTAG 1 cut(s) 296
BfrI CTTAAG 1 cut(s) 128
BlpI GCTNAGC 1 cut(s) 303
BmcAI AGTACT 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 345
BmiI GGNNCC 2 cut(s) 339, 385
BmrFI CCNGG 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 146
Bpu1102I GCTNAGC 1 cut(s) 303
BpuEI CTTGAG 1 cut(s) 303
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bse1I ACTGG 1 cut(s) 385
BseBI CCWGG 1 cut(s) 345
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 1 cut(s) 385
BshFI GGCC 2 cut(s) 54, 277
BshNI GGYRCC 2 cut(s) 337, 383
BsiHKAI GWGCWC 1 cut(s) 96
BsnI GGCC 2 cut(s) 54, 277
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 2 cut(s) 34, 403
Bsp1720I GCTNAGC 1 cut(s) 303
BspACI CCGC 1 cut(s) 414
BspANI GGCC 2 cut(s) 54, 277
BspCNI CTCAG 1 cut(s) 316
BspLI GGNNCC 2 cut(s) 339, 385
BspPI GGATC 1 cut(s) 398
BspT107I GGYRCC 2 cut(s) 337, 383
BspTI CTTAAG 1 cut(s) 128
BsrI ACTGG 1 cut(s) 385
BssMI GATC 2 cut(s) 34, 403
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 88, 113
BstAFI CTTAAG 1 cut(s) 128
BstC8I GCNNGC 2 cut(s) 52, 445
BstDEI CTNAG 1 cut(s) 303
BstKTI GATC 2 cut(s) 37, 406
BstMBI GATC 2 cut(s) 34, 403
BstNI CCWGG 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 343
BstV2I GAAGAC 1 cut(s) 146
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BsuRI GGCC 2 cut(s) 54, 277
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 2 cut(s) 369, 430
Cac8I GCNNGC 2 cut(s) 52, 445
Csp6I GTAC 1 cut(s) 25
CviAII CATG 4 cut(s) 313, 388, 418, 454
CviJI RGCY 8 cut(s) 54, 94, 127, 277, 302, 307, 317, 323
CviKI_1 RGCY 8 cut(s) 54, 94, 127, 277, 302, 307, 317, 323
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 1 cut(s) 303
DpnI GATC 2 cut(s) 36, 405
DpnII GATC 2 cut(s) 34, 403
EaeI YGGCCR 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 96
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 96
FaeI CATG 4 cut(s) 316, 391, 421, 457
FatI CATG 4 cut(s) 312, 387, 417, 453
FriOI GRGCYC 1 cut(s) 96
FspBI CTAG 1 cut(s) 296
HaeIII GGCC 2 cut(s) 54, 277
Hin1II CATG 4 cut(s) 316, 391, 421, 457
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HindIII AAGCTT 1 cut(s) 321
HphI GGTGA 1 cut(s) 211
Hpy166II GTNNAC 1 cut(s) 252
Hpy188I TCNGA 2 cut(s) 34, 104
Hpy188III TCNNGA 2 cut(s) 136, 355
Hpy8I GTNNAC 1 cut(s) 252
HpyAV CCTTC 3 cut(s) 190, 235, 328
HpyCH4III ACNGT 2 cut(s) 88, 113
HpyCH4V TGCA 1 cut(s) 374
HpyF3I CTNAG 1 cut(s) 303
Hsp92II CATG 4 cut(s) 316, 391, 421, 457
Kzo9I GATC 2 cut(s) 34, 403
LmnI GCTCC 1 cut(s) 91
LpnPI CCDG 7 cut(s) 36, 195, 240, 330, 340, 357, 366
MaeI CTAG 1 cut(s) 296
MaeIII GTNAC 1 cut(s) 205
MalI GATC 2 cut(s) 36, 405
MboI GATC 2 cut(s) 34, 403
MboII GAAGA 5 cut(s) 82, 141, 151, 250, 413
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 1 cut(s) 96
MlsI TGGCCA 1 cut(s) 54
MluCI AATT 1 cut(s) 77
MluNI TGGCCA 1 cut(s) 54
MnlI CCTC 2 cut(s) 5, 351
Mox20I TGGCCA 1 cut(s) 54
MscI TGGCCA 1 cut(s) 54
MseI TTAA 4 cut(s) 129, 155, 165, 180
MslI CAYNNNNRTG 1 cut(s) 416
Msp20I TGGCCA 1 cut(s) 54
MspCI CTTAAG 1 cut(s) 128
MspR9I CCNGG 1 cut(s) 345
MvaI CCWGG 1 cut(s) 345
NdeII GATC 2 cut(s) 34, 403
NlaIII CATG 4 cut(s) 316, 391, 421, 457
NlaIV GGNNCC 2 cut(s) 339, 385
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 2 cut(s) 339, 385
PsuI RGATCY 1 cut(s) 403
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
RseI CAYNNNNRTG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 4 cut(s) 129, 155, 165, 180
Sau3AI GATC 2 cut(s) 34, 403
ScaI AGTACT 1 cut(s) 26
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 1 cut(s) 96
SetI ASST 7 cut(s) 96, 129, 135, 201, 234, 325, 343
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 128, 318
SmoI CTYRAG 2 cut(s) 128, 318
Sse9I AATT 1 cut(s) 77
SsiI CCGC 1 cut(s) 414
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 2 cut(s) 88, 113
TasI AATT 1 cut(s) 77
TatI WGTACW 1 cut(s) 24
Tru1I TTAA 4 cut(s) 129, 155, 165, 180
Tru9I TTAA 4 cut(s) 129, 155, 165, 180
TscAI CASTG 2 cut(s) 376, 430
TspDTI ATGAA 2 cut(s) 414, 444
TspRI CASTG 2 cut(s) 376, 430
Vha464I CTTAAG 1 cut(s) 128
XspI CTAG 1 cut(s) 296
ZrmI AGTACT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.