Rorug02G0197200

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0014574
Physical Location & Seq
Forward (+)
18361803 .. 18365628
3826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0197200.1

Sequence Viewer

Length: 1365 bp
ATGAGTCACCTATATGAGGATGAAGAGGATTACGAAGAAAATGGATATGACGATAGTGAGACACAAGATGTTGGTGGCAACTTTAATATTCAACAAGACGACGACAGCGAGACACAAGATGTTGGTGGCATATTTCATCAGCAAGATGACGTCAATGTTTCATCAGAGGCTCCTGGGGGAATAAAGAAGCACCGTGGTCCAAACCAGAAGAATTGGGCAATGGAGGGTCGGGAAAAGCTCTTGTGGAATCGTTTTGGACTTCCTAAGGGTCCACGATTCAAGGTGGCAAGATTCTCAAGATTCTTGGGTATTTTGGCAAAAGACTTTACACTTTTTCCAATTAGTACACCTGACTACCGGCATTTCAATGCTAGTGACAATCTTGAAAGGGCTTGGAATCGTGTTAAGGAAACTATTGATTGGTCTAATCCTTGGGTTGCAAGTTTGGAAGGTAAAATCAAGAAAAGGGTGAAGAGCAAGTTAAATGAACGGTGGAAGCACTGGAAGTCTCAGTTGCGCAAAAAATGGTATAAACCATTCATGAAAACACCACGTAGGTTTCAAAAGCCTAATGATGATTGTGTTAATGAGCGTCAATGGAAAATTTTTGTTGCAAACATGGACAAGAAGAAGCATCAGAGAATAGCGAGCATCAACAAAAGGAATCGGTCTCAAAAGAAGATGAATCAATCAACAGGAACTCGTACCTATGCAAATGTGGCTCATGAATATGAAATTTCTCATGGTAAAGAGCCAGATAGGTGGGAGCTATTTAAATTAACACATCAAAGGAAAGGGTCCCAAGAACCGATCGATGTGGCATCTGCAAAGGCAATTGCGGATTTTGAAGAGGCTGAACAAAAGAGGCTAAGCATGAATGTTGACATCACTGCTCCAGAAATCCGTGAAGAAATGTATGCTGAAGTTCTTGGTAAGGAGAAGGGGAACAAAGTGAGGGGTGTTGGAGCAGGCGTTACTTGGGATGAAGTCCCTGGAATCCATGTTGAAGAGCGAGGAGTATCAAGAGAAGTGAAACAGTTGCGAGAGCAATTGGAAGAGCAAGTGAAAAAGGCACAAGAGAGAGAAGCACACATGATGGAGGAACTACAAAGCAAAATGGCTGAGCAAACAAAACGGATGGAACAAATGTTTGAAGTGAGGTGCGTAGAGATGGCACTCCAAAAAATGGGAGAGATGTTTAAACAATGCGGAATATCAGTGGATACACAAGAAGATGTCATGCAAACGATGTCTCAGTTTGCTCGTGACACGTCTCAAAGGCCATCGAATGTTGCTTTTTCACCAGATGATGATGTCTACCGTCCCACAATGCCATTTGATTGCCCCAATATGCAAATTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

454

Amino Acids

53.12

Weight (kDa)

6.87

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 196 - 326 3.7e-17 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014574)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39700
fragaria_vesca FvH4_1g21640
malus_domestica MD01G1024200.v1.1 MD15G1328000.v1.1
prunus_persica Prupe.6G179300_v2.0.a1
pyrus_communis pycom01g05190 pycom15g29660
rosa_chinensis RchiOBHm_Chr2g0115511
rosa_laevigata RLG00000018189
rosa_multiflora Rmu_sc0008334.1_g000016
rosa_roxburghii Rroxscaffold_2G00129020
rosa_rugosa Rorug02G0197200
rosa_samantha Rh2AG254800 Rh2BG265500 Rh2CG260000 Rh2DG262900
rosa_wichuraiana Rw2G019940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 153
Acc16I TGCGCA 1 cut(s) 518
AccB7I CCANNNNNTGG 1 cut(s) 1186
AccI GTMKAC 1 cut(s) 1317
AciI CCGC 2 cut(s) 839, 1209
AcsI RAATTY 2 cut(s) 603, 735
AcuI CTGAAG 1 cut(s) 942
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 2 cut(s) 346, 706
AfiI CCNNNNNNNGG 2 cut(s) 16, 1186
AflIII ACRYGT 1 cut(s) 1269
AgsI TTSAA 8 cut(s) 92, 280, 367, 386, 563, 848, 1007, 1154
AjiI CACGTC 1 cut(s) 1272
AjnI CCWGG 2 cut(s) 172, 991
AluBI AGCT 2 cut(s) 238, 769
AluI AGCT 2 cut(s) 238, 769
Alw26I GTCTC 6 cut(s) 53, 104, 513, 675, 1257, 1278
AoxI GGCC 1 cut(s) 1280
ApoI RAATTY 2 cut(s) 603, 735
AspLEI GCGC 1 cut(s) 519
AspS9I GGNCC 3 cut(s) 197, 269, 798
AsuHPI GGTGA 2 cut(s) 481, 1293
AvaII GGWCC 3 cut(s) 197, 269, 798
AxyI CCTNAGG 1 cut(s) 264
BauI CACGAG 1 cut(s) 1263
BccI CCATC 4 cut(s) 1090, 1132, 1165, 1291
BciT130I CCWGG 2 cut(s) 174, 993
BciVI GTATCC 1 cut(s) 1216
BcoDI GTCTC 6 cut(s) 53, 104, 513, 675, 1257, 1278
BfaI CTAG 1 cut(s) 372
BfuI GTATCC 1 cut(s) 1216
BlpI GCTNAGC 2 cut(s) 869, 1122
Bme1390I CCNGG 2 cut(s) 174, 993
Bme18I GGWCC 3 cut(s) 197, 269, 798
BmgBI CACGTC 1 cut(s) 1272
BmgT120I GGNCC 3 cut(s) 197, 269, 798
BmiI GGNNCC 4 cut(s) 171, 270, 799, 800
BmrFI CCNGG 2 cut(s) 174, 993
BmsI GCATC 3 cut(s) 643, 660, 830
BpmI CTGGAG 1 cut(s) 879
Bpu1102I GCTNAGC 2 cut(s) 869, 1122
BpuEI CTTGAG 1 cut(s) 280
Bsa29I ATCGAT 1 cut(s) 813
BsaAI YACGTR 1 cut(s) 554
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 675
BsaJI CCNNGG 4 cut(s) 173, 193, 431, 991
BsaXI ACNNNNNCTCC 2 cut(s) 957, 987
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 1186
Bse118I RCCGGY 1 cut(s) 357
Bse1I ACTGG 1 cut(s) 506
Bse21I CCTNAGG 1 cut(s) 264
Bse3DI GCAATG 1 cut(s) 225
BseBI CCWGG 2 cut(s) 174, 993
BseCI ATCGAT 1 cut(s) 813
BseDI CCNNGG 4 cut(s) 173, 193, 431, 991
BseGI GGATG 3 cut(s) 25, 988, 1143
BseLI CCNNNNNNNGG 2 cut(s) 16, 1186
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 3 cut(s) 524, 1113, 1268
BseNI ACTGG 1 cut(s) 506
BseRI GAGGAG 1 cut(s) 1029
Bsh1285I CGRYCG 1 cut(s) 813
BshFI GGCC 1 cut(s) 1282
BshVI ATCGAT 1 cut(s) 813
BsiEI CGRYCG 1 cut(s) 813
BsiSI CCGG 1 cut(s) 358
BslFI GGGAC 3 cut(s) 784, 974, 1308
BslI CCNNNNNNNGG 2 cut(s) 16, 1186
BsmAI GTCTC 6 cut(s) 53, 104, 513, 675, 1257, 1278
BsmBI CGTCTC 1 cut(s) 1278
BsmFI GGGAC 3 cut(s) 784, 974, 1308
BsnI GGCC 1 cut(s) 1282
Bso31I GGTCTC 1 cut(s) 675
Bsp143I GATC 1 cut(s) 810
Bsp1720I GCTNAGC 2 cut(s) 869, 1122
BspACI CCGC 2 cut(s) 839, 1209
BspANI GGCC 1 cut(s) 1282
BspCNI CTCAG 3 cut(s) 523, 1114, 1267
BspDI ATCGAT 1 cut(s) 813
BspHI TCATGA 2 cut(s) 540, 724
BspLI GGNNCC 4 cut(s) 171, 270, 799, 800
BspQI GCTCTTC 3 cut(s) 467, 1002, 1050
BspTNI GGTCTC 1 cut(s) 675
BsrDI GCAATG 1 cut(s) 225
BsrFI RCCGGY 1 cut(s) 357
BsrI ACTGG 1 cut(s) 506
BssAI RCCGGY 1 cut(s) 357
BssECI CCNNGG 4 cut(s) 173, 193, 431, 991
BssMI GATC 1 cut(s) 810
BssNI GRCGYC 1 cut(s) 150
BssSI CACGAG 1 cut(s) 1263
BssT1I CCWWGG 1 cut(s) 431
Bst2BI CACGAG 1 cut(s) 1263
Bst2UI CCWGG 2 cut(s) 174, 993
Bst4CI ACNGT 4 cut(s) 194, 492, 1038, 1322
Bst6I CTCTTC 5 cut(s) 18, 467, 843, 1002, 1050
BstACI GRCGYC 1 cut(s) 150
BstBAI YACGTR 1 cut(s) 554
BstC8I GCNNGC 2 cut(s) 649, 970
BstDEI CTNAG 5 cut(s) 264, 510, 869, 1122, 1254
BstDSI CCRYGG 1 cut(s) 193
BstENI CCTNNNNNAGG 1 cut(s) 14
BstF5I GGATG 3 cut(s) 25, 988, 1143
BstHHI GCGC 1 cut(s) 519
BstKTI GATC 1 cut(s) 813
BstMAI GTCTC 6 cut(s) 53, 104, 513, 675, 1257, 1278
BstMBI GATC 1 cut(s) 810
BstMCI CGRYCG 1 cut(s) 813
BstMWI GCNNNNNNNGC 1 cut(s) 719
BstNI CCWGG 2 cut(s) 174, 993
BstSCI CCNGG 2 cut(s) 172, 991
BstXI CCANNNNNNTGG 1 cut(s) 762
Bsu15I ATCGAT 1 cut(s) 813
Bsu36I CCTNAGG 1 cut(s) 264
BsuI GTATCC 1 cut(s) 1216
BsuRI GGCC 1 cut(s) 1282
BsuTUI ATCGAT 1 cut(s) 813
BtgI CCRYGG 1 cut(s) 193
BtrI CACGTC 1 cut(s) 1272
BtsCI GGATG 3 cut(s) 25, 988, 1143
BtsI GCAGTG 1 cut(s) 888
BtsIMutI CAGTG 3 cut(s) 499, 888, 1224
Cac8I GCNNGC 2 cut(s) 649, 970
CciI TCATGA 2 cut(s) 540, 724
CfoI GCGC 1 cut(s) 519
Cfr10I RCCGGY 1 cut(s) 357
Cfr13I GGNCC 3 cut(s) 197, 269, 798
ClaI ATCGAT 1 cut(s) 813
CseI GACGC 1 cut(s) 581
Csp6I GTAC 2 cut(s) 345, 705
CviAII CATG 8 cut(s) 541, 619, 725, 743, 874, 1001, 1093, 1240
CviQI GTAC 2 cut(s) 345, 705
DdeI CTNAG 5 cut(s) 264, 510, 869, 1122, 1254
DpnI GATC 1 cut(s) 812
DpnII GATC 1 cut(s) 810
DraI TTTAAA 2 cut(s) 775, 1201
Eam1104I CTCTTC 5 cut(s) 18, 467, 843, 1002, 1050
EarI CTCTTC 5 cut(s) 18, 467, 843, 1002, 1050
Eco130I CCWWGG 1 cut(s) 431
Eco31I GGTCTC 1 cut(s) 675
Eco47I GGWCC 3 cut(s) 197, 269, 798
Eco57I CTGAAG 1 cut(s) 942
Eco81I CCTNAGG 1 cut(s) 264
EcoNI CCTNNNNNAGG 1 cut(s) 14
EcoO109I RGGNCCY 1 cut(s) 798
EcoRII CCWGG 2 cut(s) 172, 991
EcoT14I CCWWGG 1 cut(s) 431
ErhI CCWWGG 1 cut(s) 431
Esp3I CGTCTC 1 cut(s) 1278
FaeI CATG 8 cut(s) 544, 622, 728, 746, 877, 1004, 1096, 1243
FaqI GGGAC 3 cut(s) 784, 974, 1308
FatI CATG 8 cut(s) 540, 618, 724, 742, 873, 1000, 1092, 1239
FblI GTMKAC 1 cut(s) 1317
FokI GGATG 3 cut(s) 32, 995, 1150
FspBI CTAG 1 cut(s) 372
FspI TGCGCA 1 cut(s) 518
GlaI GCGC 1 cut(s) 518
GsuI CTGGAG 1 cut(s) 879
HaeIII GGCC 1 cut(s) 1282
HapII CCGG 1 cut(s) 358
HgaI GACGC 1 cut(s) 581
HhaI GCGC 1 cut(s) 519
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 8 cut(s) 544, 622, 728, 746, 877, 1004, 1096, 1243
Hin6I GCGC 1 cut(s) 517
HinP1I GCGC 1 cut(s) 517
HincII GTYRAC 1 cut(s) 883
HindII GTYRAC 1 cut(s) 883
HinfI GANTC 9 cut(s) 4, 247, 276, 291, 300, 397, 664, 685, 996
HpaII CCGG 1 cut(s) 358
HphI GGTGA 2 cut(s) 481, 1293
Hpy166II GTNNAC 4 cut(s) 272, 347, 883, 1318
Hpy188I TCNGA 2 cut(s) 166, 639
Hpy188III TCNNGA 9 cut(s) 230, 297, 383, 460, 541, 725, 896, 1023, 1265
Hpy8I GTNNAC 4 cut(s) 272, 347, 883, 1318
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 2 cut(s) 443, 934
HpyCH4III ACNGT 4 cut(s) 194, 492, 1038, 1322
HpyCH4IV ACGT 3 cut(s) 150, 553, 1271
HpyCH4V TGCA 6 cut(s) 440, 614, 713, 827, 1243, 1354
HpyF10VI GCNNNNNNNGC 1 cut(s) 719
HpyF3I CTNAG 5 cut(s) 264, 510, 869, 1122, 1254
HpySE526I ACGT 3 cut(s) 150, 553, 1271
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 8 cut(s) 544, 622, 728, 746, 877, 1004, 1096, 1243
HspAI GCGC 1 cut(s) 517
KflI GGGWCCC 1 cut(s) 798
Kzo9I GATC 1 cut(s) 810
LguI GCTCTTC 3 cut(s) 467, 1002, 1050
LmnI GCTCC 4 cut(s) 175, 766, 898, 965
LweI GCATC 3 cut(s) 643, 660, 830
MaeI CTAG 1 cut(s) 372
MaeII ACGT 3 cut(s) 150, 553, 1271
MaeIII GTNAC 4 cut(s) 5, 374, 973, 1265
MalI GATC 1 cut(s) 812
MboI GATC 1 cut(s) 810
MfeI CAATTG 2 cut(s) 834, 1049
MluCI AATT 8 cut(s) 211, 339, 603, 735, 776, 834, 1049, 1356
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 943
MseI TTAA 8 cut(s) 84, 405, 482, 585, 774, 779, 1200, 1363
MslI CAYNNNNRTG 3 cut(s) 12, 366, 729
MspI CCGG 1 cut(s) 358
MspR9I CCNGG 2 cut(s) 174, 993
MssI GTTTAAAC 1 cut(s) 1201
MunI CAATTG 2 cut(s) 834, 1049
MvaI CCWGG 2 cut(s) 174, 993
MwoI GCNNNNNNNGC 1 cut(s) 719
NdeII GATC 1 cut(s) 810
NlaIII CATG 8 cut(s) 544, 622, 728, 746, 877, 1004, 1096, 1243
NlaIV GGNNCC 4 cut(s) 171, 270, 799, 800
NmuCI GTSAC 3 cut(s) 5, 374, 1265
NsbI TGCGCA 1 cut(s) 518
PagI TCATGA 2 cut(s) 540, 724
PciSI GCTCTTC 3 cut(s) 467, 1002, 1050
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfeI GAWTC 8 cut(s) 247, 276, 291, 300, 397, 664, 685, 996
PflMI CCANNNNNTGG 1 cut(s) 1186
Ple19I CGATCG 1 cut(s) 813
PleI GAGTC 1 cut(s) 12
PmeI GTTTAAAC 1 cut(s) 1201
PpsI GAGTC 1 cut(s) 12
Ppu21I YACGTR 1 cut(s) 554
PpuMI RGGWCCY 1 cut(s) 798
Psp5II RGGWCCY 1 cut(s) 798
Psp6I CCWGG 2 cut(s) 172, 991
PspGI CCWGG 2 cut(s) 172, 991
PspN4I GGNNCC 4 cut(s) 171, 270, 799, 800
PspPI GGNCC 3 cut(s) 197, 269, 798
PspPPI RGGWCCY 1 cut(s) 798
PvuI CGATCG 1 cut(s) 813
RsaI GTAC 2 cut(s) 346, 706
RsaNI GTAC 2 cut(s) 345, 705
RseI CAYNNNNRTG 3 cut(s) 12, 366, 729
SapI GCTCTTC 3 cut(s) 467, 1002, 1050
SaqAI TTAA 8 cut(s) 84, 405, 482, 585, 774, 779, 1200, 1363
Sau3AI GATC 1 cut(s) 810
Sau96I GGNCC 3 cut(s) 197, 269, 798
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 2 cut(s) 174, 993
SfaNI GCATC 3 cut(s) 643, 660, 830
SinI GGWCC 3 cut(s) 197, 269, 798
SmiI ATTTAAAT 1 cut(s) 775
SmiMI CAYNNNNRTG 3 cut(s) 12, 366, 729
SmlI CTYRAG 1 cut(s) 295
SmoI CTYRAG 1 cut(s) 295
Sse9I AATT 8 cut(s) 211, 339, 603, 735, 776, 834, 1049, 1356
SsiI CCGC 2 cut(s) 839, 1209
SspI AATATT 1 cut(s) 88
SspMI CTAG 1 cut(s) 372
StyD4I CCNGG 2 cut(s) 172, 991
StyI CCWWGG 1 cut(s) 431
SwaI ATTTAAAT 1 cut(s) 775
TaaI ACNGT 4 cut(s) 194, 492, 1038, 1322
TaiI ACGT 3 cut(s) 153, 556, 1274
TaqI TCGA 2 cut(s) 813, 1286
TaqII GACCGA 1 cut(s) 657
TasI AATT 8 cut(s) 211, 339, 603, 735, 776, 834, 1049, 1356
TatI WGTACW 1 cut(s) 344
TfiI GAWTC 8 cut(s) 247, 276, 291, 300, 397, 664, 685, 996
Tru1I TTAA 8 cut(s) 84, 405, 482, 585, 774, 779, 1200, 1363
Tru9I TTAA 8 cut(s) 84, 405, 482, 585, 774, 779, 1200, 1363
TscAI CASTG 3 cut(s) 506, 895, 1224
TseFI GTSAC 3 cut(s) 5, 374, 1265
Tsp45I GTSAC 3 cut(s) 5, 374, 1265
TspGWI ACGGA 2 cut(s) 893, 1150
TspRI CASTG 3 cut(s) 506, 895, 1224
Van91I CCANNNNNTGG 1 cut(s) 1186
VpaK11BI GGWCC 3 cut(s) 197, 269, 798
XagI CCTNNNNNAGG 1 cut(s) 14
XapI RAATTY 2 cut(s) 603, 735
XmiI GTMKAC 1 cut(s) 1317
XspI CTAG 1 cut(s) 372
ZraI GACGTC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.