MD01G1024200.v1.1

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014574
Physical Location & Seq
Forward (+)
9176587 .. 9177648
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1024200.v1.1.491

Sequence Viewer

Length: 462 bp
ATGGGAGTTGGAGGAACATTGGAGTACTTGTCTGATCTGGTGAACAACAGTGGGTATAAACACAAAAAGAAGAAGCAATTACAGACAGTGGAGCTCAAAGTCAGGATGGACTGTGATGGCTGTGAACTTAAGGTCAAGAATGCCCTCTCTTCCTTAACTGGGGTTAAATCTGTGGAGATAAACAGGAAGCAACAGAAGGTGACTGTGACAGGGTATGTTGAACCAAACAAAGTGCTGAAGAAGGCAAAGTCAACAGGGAAGAGGGCGGAGATATGGCCTTATGTGCCCTACAGCTTAGTGGCACAGCCCTACATTGCTCAGGCTTATGACAAGAAGGCACCTCCTGGTTATGTCAGAAATGTGGACAACAATCCGTCCTCCACTGGGACTGTCACAAAATATGAAGACCCTTACACCACAATGTTCAGTGATGACAACCCTAATGCCTGCTCCATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

154

Amino Acids

17.0

Weight (kDa)

9.32

Isoelectric Point (pI)

27.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 33 - 87 1.4e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014574)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39700
fragaria_vesca FvH4_1g21640
malus_domestica MD01G1024200.v1.1 MD15G1328000.v1.1
prunus_persica Prupe.6G179300_v2.0.a1
pyrus_communis pycom01g05190 pycom15g29660
rosa_chinensis RchiOBHm_Chr2g0115511
rosa_laevigata RLG00000018189
rosa_multiflora Rmu_sc0008334.1_g000016
rosa_roxburghii Rroxscaffold_2G00129020
rosa_rugosa Rorug02G0197200
rosa_samantha Rh2AG254800 Rh2BG265500 Rh2CG260000 Rh2DG262900
rosa_wichuraiana Rw2G019940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 337
AciI CCGC 1 cut(s) 266
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 1 cut(s) 26
AfiI CCNNNNNNNGG 2 cut(s) 159, 384
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 1 cut(s) 221
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 2 cut(s) 94, 294
AluI AGCT 2 cut(s) 94, 294
Alw21I GWGCWC 1 cut(s) 96
AoxI GGCC 1 cut(s) 275
AsuHPI GGTGA 2 cut(s) 52, 211
BaeGI GKGCMC 1 cut(s) 288
BanI GGYRCC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 96
BccI CCATC 3 cut(s) 100, 110, 461
BciT130I CCWGG 1 cut(s) 345
BfmI CTRYAG 1 cut(s) 289
BfrI CTTAAG 1 cut(s) 128
BmcAI AGTACT 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 345
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 1 cut(s) 345
BmrI ACTGGG 2 cut(s) 168, 393
BmuI ACTGGG 2 cut(s) 168, 393
BpiI GAAGAC 1 cut(s) 411
Bpu10I CCTNAGC 1 cut(s) 318
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 384
Bse1I ACTGG 2 cut(s) 163, 388
Bse3DI GCAATG 1 cut(s) 312
BseBI CCWGG 1 cut(s) 345
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 2 cut(s) 159, 384
BseMI GCAATG 1 cut(s) 312
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 2 cut(s) 163, 388
BseSI GKGCMC 1 cut(s) 288
BshFI GGCC 1 cut(s) 277
BshNI GGYRCC 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 96
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 2 cut(s) 159, 384
BsmFI GGGAC 1 cut(s) 400
BsmI GAATGC 1 cut(s) 145
BsnI GGCC 1 cut(s) 277
Bsp1286I GDGCHC 2 cut(s) 96, 288
Bsp143I GATC 1 cut(s) 34
BspACI CCGC 1 cut(s) 266
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 1 cut(s) 331
BspLI GGNNCC 1 cut(s) 339
BspT107I GGYRCC 1 cut(s) 337
BspTI CTTAAG 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 312
BsrI ACTGG 2 cut(s) 163, 388
BssMI GATC 1 cut(s) 34
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 5 cut(s) 50, 88, 113, 205, 391
Bst6I CTCTTC 2 cut(s) 154, 254
BstAFI CTTAAG 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 448
BstDEI CTNAG 2 cut(s) 295, 318
BstF5I GGATG 1 cut(s) 111
BstKTI GATC 1 cut(s) 37
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 1 cut(s) 283
BstNI CCWGG 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 343
BstSFI CTRYAG 1 cut(s) 289
BstSLI GKGCMC 1 cut(s) 288
BstV2I GAAGAC 1 cut(s) 411
BsuRI GGCC 1 cut(s) 277
BtsCI GGATG 1 cut(s) 111
BtsIMutI CAGTG 4 cut(s) 55, 93, 381, 433
Cac8I GCNNGC 1 cut(s) 448
Csp6I GTAC 1 cut(s) 25
CviAII CATG 1 cut(s) 457
CviJI RGCY 6 cut(s) 94, 120, 277, 294, 307, 323
CviKI_1 RGCY 6 cut(s) 94, 120, 277, 294, 307, 323
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 2 cut(s) 295, 318
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 154, 254
EarI CTCTTC 2 cut(s) 154, 254
EciI GGCGGA 1 cut(s) 281
Ecl136II GAGCTC 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 96
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 96
FaeI CATG 1 cut(s) 460
FaiI YATR 8 cut(s) 57, 216, 274, 282, 327, 351, 402, 458
FaqI GGGAC 1 cut(s) 400
FatI CATG 1 cut(s) 456
FokI GGATG 1 cut(s) 118
FriOI GRGCYC 1 cut(s) 96
HaeIII GGCC 1 cut(s) 277
Hin1II CATG 1 cut(s) 460
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HphI GGTGA 2 cut(s) 52, 211
Hpy166II GTNNAC 4 cut(s) 43, 125, 252, 364
Hpy188I TCNGA 2 cut(s) 34, 356
Hpy188III TCNNGA 2 cut(s) 103, 136
Hpy8I GTNNAC 4 cut(s) 43, 125, 252, 364
HpyAV CCTTC 3 cut(s) 190, 235, 328
HpyCH4III ACNGT 5 cut(s) 50, 88, 113, 205, 391
HpyF10VI GCNNNNNNNGC 1 cut(s) 283
HpyF3I CTNAG 2 cut(s) 295, 318
Hsp92II CATG 1 cut(s) 460
Kzo9I GATC 1 cut(s) 34
LmnI GCTCC 2 cut(s) 91, 455
MaeIII GTNAC 3 cut(s) 199, 205, 391
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MboII GAAGA 5 cut(s) 82, 141, 250, 271, 416
MhlI GDGCHC 2 cut(s) 96, 288
MluCI AATT 1 cut(s) 77
MnlI CCTC 5 cut(s) 5, 155, 255, 351, 388
MseI TTAA 3 cut(s) 129, 155, 165
MslI CAYNNNNRTG 1 cut(s) 419
MspCI CTTAAG 1 cut(s) 128
MspR9I CCNGG 1 cut(s) 345
Mva1269I GAATGC 1 cut(s) 145
MvaI CCWGG 1 cut(s) 345
MwoI GCNNNNNNNGC 1 cut(s) 283
NdeII GATC 1 cut(s) 34
NlaIII CATG 1 cut(s) 460
NlaIV GGNNCC 1 cut(s) 339
NmuCI GTSAC 3 cut(s) 199, 205, 391
PctI GAATGC 1 cut(s) 145
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 1 cut(s) 339
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
RseI CAYNNNNRTG 1 cut(s) 419
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 3 cut(s) 129, 155, 165
Sau3AI GATC 1 cut(s) 34
ScaI AGTACT 1 cut(s) 26
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 2 cut(s) 96, 288
SetI ASST 5 cut(s) 96, 135, 201, 296, 343
SfcI CTRYAG 1 cut(s) 289
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 1 cut(s) 77
SsiI CCGC 1 cut(s) 266
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 5 cut(s) 50, 88, 113, 205, 391
TasI AATT 1 cut(s) 77
TatI WGTACW 1 cut(s) 24
Tru1I TTAA 3 cut(s) 129, 155, 165
Tru9I TTAA 3 cut(s) 129, 155, 165
TscAI CASTG 4 cut(s) 55, 93, 388, 433
TseFI GTSAC 3 cut(s) 199, 205, 391
Tsp45I GTSAC 3 cut(s) 199, 205, 391
TspDTI ATGAA 1 cut(s) 417
TspGWI ACGGA 1 cut(s) 363
TspRI CASTG 4 cut(s) 55, 93, 388, 433
Vha464I CTTAAG 1 cut(s) 128
ZrmI AGTACT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.