FvH4_1g24350

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0016048
Physical Location & Seq
Reverse (-)
16228349 .. 16233732
5384 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24350.t1

Sequence Viewer

Length: 1446 bp
ATGGATTCGGAGAAGTCCATATTCGGCGGCGCCATCAGGCCGACGTGGCCGTCTCTGCTCCTCCTCTTCACCATCATGCTCCTCCTCTCCTACCAAATCTCCACCATGGCTGCCCTGCCGCGCTGGGCCGGGTCGGTCTGGGGATCCGAAACCGCTTTGACCGACCCGACGAGGAGGAGCTGCTCCGGATTCTTCGGCGAGGACGGAAGAGCTCCGCGGAGGAGGAGGAGGAAGACGGTGATGTCGATAAGAGACTTCGGCGGAGTCGGCGACGGGACGACGTCGAATACGGCGGCGTTTAGGAAGGCGATACGGTTCATGCAGGAGCAGCAAGGTGGGGCCCAGCTGAATGTTCCCGAAGGGAGATGGTTGACGGGGAGTTTTAACCTCACCAGTAACTTCACGCTTTTTCTCGAGCACGGCGCTGTAATTCTCGGCTCCCAGGATCCAAAGGAATGGCCTATAATTGAGCCATTGCCTTCTTATGGAAGAGGGAGAGAGCGGTTAGGAGGAAGGCATATAAGCCTTATTCATGGGGACGGTCTCACTGATGTTGTCATTACAGGTCATAATGGAACAATTGACGGTCAAGGAAAAATGTGGTGGGAACTATGGTGGAACAGAACGCTCGAGTATACGAGAGGTCACCTTCTTGAACTAATGAACTCTAACAACATTCTCATCTCTAACCTCACCTTCCTCAATTCTCCTTTTTGGACAGTTCATCCTGTTTACTGCAGCAATGTTGTTATAAAGGACATGACAATTTTGGCTCCCCTTAAAGCTCCTAACACTGACGGCATTGACGCAGATTCATGTACAAACGTGTGCATTGAAGATTGTTACATTGAGAGTGGCGACGATCTTATAGCCATTAAAAGTGGTTGGGATCACTATGGAATCAAGATGGCTCGTCCAAGCTCAAACATCATAGTGAGGAGAGTTTCTGGCACCACTCCCACCTGTTCTGGAATTGGAATAGGTAGTGAGATGTCTGGTGGGATTTCAAATGTTACCATTGAGGATTTGCATGTTTGGAATTCTGCAGCTGGGGTGCGTATAAAATCTGATAAGGGTCGAGGGGGTTACATAGAAAATGTTACTATAAGTAACATAAAAATGGAAAGGGTCAAGACACCCATAAAGTTTAGTAGAGGGTCCAATGACCACCCGGACAAAGGATGGGATCCCAAAGCTGTTCCCAAAGTAAAGGGAATTTTCATCAGCAATGTTCTGAGCTTAAACTCAACAAAAGCCCCTGTACTGCTGGGTGTCGAGGGTGGTTCTTATGAAGGGATCTGTCTCAGGAATGTAACTGTACTTGGTTTAGCAGCATCTGCAGCGTGGCAATGTGAATTTGTTTCCGGTTTTGCTAATGACGTATTTCCAACGCCATGTCCTCAGATGCAGGATATTGGCTCTTTTGACTGGTGTCAGCAACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

482

Amino Acids

52.78

Weight (kDa)

8.0

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 176 - 454 1.2e-44 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33440
fragaria_vesca FvH4_1g24350
malus_domestica MD15G1441700.v1.1
prunus_persica Prupe.4G271300_v2.0.a1
pyrus_communis pycom15g38970
rosa_chinensis RchiOBHm_Chr2g0120901
rosa_laevigata RLG00000018600
rosa_multiflora Rmu_sc0000621.1_g000016
rosa_roxburghii Rroxscaffold_2G00122750
rosa_rugosa Rorug02G0232600
rosa_samantha Rh2AG289000 Rh2BG300000 Rh2CG278000 Rh2DG314000
rosa_wichuraiana Rw2G023150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 752
AasI GACNNNNNNGTC 2 cut(s) 49, 241
AatII GACGTC 1 cut(s) 284
AccB1I GGYRCC 2 cut(s) 29, 950
AccBSI CCGCTC 1 cut(s) 502
AccI GTMKAC 1 cut(s) 635
AccII CGCG 2 cut(s) 121, 217
AccIII TCCGGA 1 cut(s) 185
AciI CCGC 8 cut(s) 27, 119, 153, 215, 217, 261, 293, 502
AclI AACGTT 1 cut(s) 1441
AclWI GGATC 8 cut(s) 138, 151, 440, 453, 897, 1181, 1194, 1304
AcoI YGGCCR 1 cut(s) 47
AcsI RAATTY 3 cut(s) 1039, 1215, 1355
AcyI GRCGYC 2 cut(s) 30, 281
AfaI GTAC 3 cut(s) 820, 1263, 1320
AfiI CCNNNNNNNGG 2 cut(s) 485, 1178
AflIII ACRYGT 1 cut(s) 825
AgsI TTSAA 3 cut(s) 656, 836, 1008
AjiI CACGTC 1 cut(s) 45
AjnI CCWGG 1 cut(s) 441
AluBI AGCT 8 cut(s) 180, 212, 346, 785, 921, 1049, 1196, 1239
AluI AGCT 8 cut(s) 180, 212, 346, 785, 921, 1049, 1196, 1239
Alw21I GWGCWC 2 cut(s) 214, 420
Alw26I GTCTC 4 cut(s) 57, 246, 548, 1307
AlwI GGATC 8 cut(s) 138, 151, 440, 453, 897, 1181, 1194, 1304
AlwNI CAGNNNCTG 1 cut(s) 1337
Ama87I CYCGRG 2 cut(s) 413, 629
Aor13HI TCCGGA 1 cut(s) 185
AoxI GGCC 5 cut(s) 38, 47, 126, 339, 458
ApaI GGGCCC 1 cut(s) 343
ApeKI GCWGC 7 cut(s) 110, 180, 328, 738, 1046, 1331, 1340
ApoI RAATTY 3 cut(s) 1039, 1215, 1355
AspLEI GCGC 3 cut(s) 32, 123, 425
AspS9I GGNCC 4 cut(s) 126, 339, 340, 1158
AsuC2I CCSGG 2 cut(s) 130, 1172
AsuHPI GGTGA 5 cut(s) 61, 250, 382, 638, 685
AvaI CYCGRG 2 cut(s) 413, 629
AvaII GGWCC 1 cut(s) 1158
BaeGI GKGCMC 1 cut(s) 343
BamHI GGATCC 3 cut(s) 143, 445, 1186
BanI GGYRCC 2 cut(s) 29, 950
BanII GRGCYC 2 cut(s) 214, 343
BbsI GAAGAC 1 cut(s) 239
Bbv12I GWGCWC 2 cut(s) 214, 420
BbvI GCAGC 7 cut(s) 97, 167, 340, 750, 1058, 1343, 1352
BccI CCATC 5 cut(s) 41, 80, 360, 901, 1176
BceAI ACGGC 4 cut(s) 34, 306, 436, 814
BciT130I CCWGG 1 cut(s) 443
BcnI CCSGG 2 cut(s) 130, 1172
BcoDI GTCTC 4 cut(s) 57, 246, 548, 1307
BfmI CTRYAG 3 cut(s) 736, 1044, 1338
BfoI RGCGCY 2 cut(s) 33, 426
BglI GCCNNNNNGGC 1 cut(s) 46
Bme1390I CCNGG 3 cut(s) 130, 443, 1172
Bme18I GGWCC 1 cut(s) 1158
BmeT110I CYCGRG 2 cut(s) 413, 629
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 4 cut(s) 126, 339, 340, 1158
BmrFI CCNGG 3 cut(s) 130, 443, 1172
BmsI GCATC 2 cut(s) 1343, 1395
BoxI GACNNNNGTC 1 cut(s) 1431
BpiI GAAGAC 1 cut(s) 239
BpuMI CCSGG 2 cut(s) 130, 1172
BsaHI GRCGYC 2 cut(s) 30, 281
BsaI GGTCTC 1 cut(s) 548
BsaJI CCNNGG 3 cut(s) 105, 215, 441
BsaWI WCCGGW 2 cut(s) 185, 1364
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 2 cut(s) 485, 1178
Bse1I ACTGG 2 cut(s) 393, 1433
Bse3DI GCAATG 4 cut(s) 473, 748, 1234, 1355
BseAI TCCGGA 1 cut(s) 185
BseBI CCWGG 1 cut(s) 443
BseDI CCNNGG 3 cut(s) 105, 215, 441
BseGI GGATG 2 cut(s) 724, 1187
BseLI CCNNNNNNNGG 2 cut(s) 485, 1178
BseMI GCAATG 4 cut(s) 473, 748, 1234, 1355
BseMII CTCAG 3 cut(s) 1226, 1318, 1415
BseNI ACTGG 2 cut(s) 393, 1433
BseSI GKGCMC 1 cut(s) 343
BseXI GCAGC 7 cut(s) 97, 167, 340, 750, 1058, 1343, 1352
BseYI CCCAGC 4 cut(s) 123, 342, 1049, 1267
Bsh1236I CGCG 2 cut(s) 121, 217
BshFI GGCC 5 cut(s) 40, 49, 128, 341, 460
BshNI GGYRCC 2 cut(s) 29, 950
BsiHKAI GWGCWC 2 cut(s) 214, 420
BsiHKCI CYCGRG 2 cut(s) 413, 629
BsiSI CCGG 4 cut(s) 129, 186, 1172, 1365
BslFI GGGAC 2 cut(s) 289, 551
BslI CCNNNNNNNGG 2 cut(s) 485, 1178
BsmAI GTCTC 4 cut(s) 57, 246, 548, 1307
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 2 cut(s) 289, 551
BsnI GGCC 5 cut(s) 40, 49, 128, 341, 460
Bso31I GGTCTC 1 cut(s) 548
BsoBI CYCGRG 2 cut(s) 413, 629
Bsp120I GGGCCC 1 cut(s) 339
Bsp1286I GDGCHC 3 cut(s) 214, 343, 420
Bsp13I TCCGGA 1 cut(s) 185
Bsp1407I TGTACA 1 cut(s) 818
Bsp143I GATC 6 cut(s) 143, 445, 862, 889, 1186, 1296
Bsp19I CCATGG 1 cut(s) 105
BspACI CCGC 8 cut(s) 27, 119, 153, 215, 217, 261, 293, 502
BspANI GGCC 5 cut(s) 40, 49, 128, 341, 460
BspCNI CTCAG 3 cut(s) 1227, 1317, 1414
BspEI TCCGGA 1 cut(s) 185
BspFNI CGCG 2 cut(s) 121, 217
BspMAI CTGCAG 3 cut(s) 740, 1048, 1342
BspPI GGATC 8 cut(s) 138, 151, 440, 453, 897, 1181, 1194, 1304
BspQI GCTCTTC 1 cut(s) 202
BspT107I GGYRCC 2 cut(s) 29, 950
BspTNI GGTCTC 1 cut(s) 548
BsrBI CCGCTC 1 cut(s) 502
BsrDI GCAATG 4 cut(s) 473, 748, 1234, 1355
BsrGI TGTACA 1 cut(s) 818
BsrI ACTGG 2 cut(s) 393, 1433
BssECI CCNNGG 3 cut(s) 105, 215, 441
BssMI GATC 6 cut(s) 143, 445, 862, 889, 1186, 1296
BssNAI GTATAC 1 cut(s) 636
BssNI GRCGYC 2 cut(s) 30, 281
BssT1I CCWWGG 1 cut(s) 105
Bst1107I GTATAC 1 cut(s) 636
Bst2UI CCWGG 1 cut(s) 443
Bst4CI ACNGT 6 cut(s) 238, 315, 542, 587, 721, 1318
Bst6I CTCTTC 3 cut(s) 71, 202, 484
BstACI GRCGYC 2 cut(s) 30, 281
BstAPI GCANNNNNTGC 1 cut(s) 1337
BstAUI TGTACA 1 cut(s) 818
BstDEI CTNAG 3 cut(s) 1235, 1304, 1401
BstDSI CCRYGG 2 cut(s) 105, 215
BstEII GGTNACC 1 cut(s) 644
BstF5I GGATG 2 cut(s) 724, 1187
BstFNI CGCG 2 cut(s) 121, 217
BstH2I RGCGCY 2 cut(s) 33, 426
BstHHI GCGC 3 cut(s) 32, 123, 425
BstKTI GATC 6 cut(s) 146, 448, 865, 892, 1189, 1299
BstMAI GTCTC 4 cut(s) 57, 246, 548, 1307
BstMBI GATC 6 cut(s) 143, 445, 862, 889, 1186, 1296
BstMWI GCNNNNNNNGC 6 cut(s) 46, 55, 267, 328, 1337, 1340
BstNI CCWGG 1 cut(s) 443
BstNSI RCATGY 1 cut(s) 1034
BstPAI GACNNNNGTC 1 cut(s) 1431
BstPI GGTNACC 1 cut(s) 644
BstSCI CCNGG 3 cut(s) 128, 441, 1170
BstSFI CTRYAG 3 cut(s) 736, 1044, 1338
BstSLI GKGCMC 1 cut(s) 343
BstUI CGCG 2 cut(s) 121, 217
BstV1I GCAGC 7 cut(s) 97, 167, 340, 750, 1058, 1343, 1352
BstV2I GAAGAC 1 cut(s) 239
BstX2I RGATCY 4 cut(s) 143, 445, 1186, 1296
BstXI CCANNNNNNTGG 1 cut(s) 456
BstYI RGATCY 4 cut(s) 143, 445, 1186, 1296
BstZ17I GTATAC 1 cut(s) 636
BsuRI GGCC 5 cut(s) 40, 49, 128, 341, 460
BtgI CCRYGG 2 cut(s) 105, 215
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 2 cut(s) 724, 1187
BtsIMutI CAGTG 2 cut(s) 546, 792
CaiI CAGNNNCTG 1 cut(s) 1337
CfoI GCGC 3 cut(s) 32, 123, 425
Cfr13I GGNCC 4 cut(s) 126, 339, 340, 1158
Cfr42I CCGCGG 1 cut(s) 218
CseI GACGC 1 cut(s) 815
Csp6I GTAC 3 cut(s) 819, 1262, 1319
CviAII CATG 8 cut(s) 76, 106, 319, 533, 760, 816, 1031, 1395
CviQI GTAC 3 cut(s) 819, 1262, 1319
DdeI CTNAG 3 cut(s) 1235, 1304, 1401
DinI GGCGCC 1 cut(s) 31
DpnI GATC 6 cut(s) 145, 447, 864, 891, 1188, 1298
DpnII GATC 6 cut(s) 143, 445, 862, 889, 1186, 1296
DrdI GACNNNNNNGTC 2 cut(s) 49, 241
DseDI GACNNNNNNGTC 2 cut(s) 49, 241
EaeI YGGCCR 1 cut(s) 47
Eam1104I CTCTTC 3 cut(s) 71, 202, 484
EarI CTCTTC 3 cut(s) 71, 202, 484
EciI GGCGGA 1 cut(s) 276
Ecl136II GAGCTC 1 cut(s) 212
Eco130I CCWWGG 1 cut(s) 105
Eco24I GRGCYC 2 cut(s) 214, 343
Eco31I GGTCTC 1 cut(s) 548
Eco47I GGWCC 1 cut(s) 1158
Eco53kI GAGCTC 1 cut(s) 212
Eco88I CYCGRG 2 cut(s) 413, 629
Eco91I GGTNACC 1 cut(s) 644
EcoICRI GAGCTC 1 cut(s) 212
EcoO109I RGGNCCY 1 cut(s) 339
EcoO65I GGTNACC 1 cut(s) 644
EcoRI GAATTC 1 cut(s) 1039
EcoRII CCWGG 1 cut(s) 441
EcoT14I CCWWGG 1 cut(s) 105
EcoT38I GRGCYC 2 cut(s) 214, 343
EgeI GGCGCC 1 cut(s) 31
EheI GGCGCC 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 105
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 8 cut(s) 79, 109, 322, 536, 763, 819, 1034, 1398
FaqI GGGAC 2 cut(s) 289, 551
FatI CATG 8 cut(s) 75, 105, 318, 532, 759, 815, 1030, 1394
FblI GTMKAC 1 cut(s) 635
FokI GGATG 2 cut(s) 711, 1194
FriOI GRGCYC 2 cut(s) 214, 343
GlaI GCGC 3 cut(s) 31, 122, 424
GsaI CCCAGC 4 cut(s) 127, 346, 1053, 1271
HaeII RGCGCY 2 cut(s) 33, 426
HaeIII GGCC 5 cut(s) 40, 49, 128, 341, 460
HapII CCGG 4 cut(s) 129, 186, 1172, 1365
HgaI GACGC 1 cut(s) 815
HhaI GCGC 3 cut(s) 32, 123, 425
Hin1I GRCGYC 2 cut(s) 30, 281
Hin1II CATG 8 cut(s) 79, 109, 322, 536, 763, 819, 1034, 1398
Hin6I GCGC 3 cut(s) 30, 121, 423
HinP1I GCGC 3 cut(s) 30, 121, 423
HincII GTYRAC 1 cut(s) 372
HindII GTYRAC 1 cut(s) 372
HinfI GANTC 5 cut(s) 5, 189, 264, 812, 900
HpaII CCGG 4 cut(s) 129, 186, 1172, 1365
HphI GGTGA 5 cut(s) 61, 250, 382, 638, 685
Hpy166II GTNNAC 3 cut(s) 372, 636, 733
Hpy188I TCNGA 5 cut(s) 10, 148, 1069, 1236, 1404
Hpy188III TCNNGA 8 cut(s) 186, 356, 413, 653, 904, 969, 1132, 1306
Hpy8I GTNNAC 3 cut(s) 372, 636, 733
Hpy99I CGWCG 6 cut(s) 46, 172, 275, 283, 286, 863
HpyAV CCTTC 7 cut(s) 298, 353, 489, 507, 659, 706, 1286
HpyCH4III ACNGT 6 cut(s) 238, 315, 542, 587, 721, 1318
HpyCH4IV ACGT 5 cut(s) 44, 281, 825, 1380, 1441
HpyCH4V TGCA 7 cut(s) 322, 738, 831, 1030, 1046, 1340, 1408
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 55, 267, 328, 1337, 1340
HpyF3I CTNAG 3 cut(s) 1235, 1304, 1401
HpySE526I ACGT 5 cut(s) 44, 281, 825, 1380, 1441
Hsp92I GRCGYC 2 cut(s) 30, 281
Hsp92II CATG 8 cut(s) 79, 109, 322, 536, 763, 819, 1034, 1398
HspAI GCGC 3 cut(s) 30, 121, 423
KasI GGCGCC 1 cut(s) 29
Kpn2I TCCGGA 1 cut(s) 185
KspI CCGCGG 1 cut(s) 218
Kzo9I GATC 6 cut(s) 143, 445, 862, 889, 1186, 1296
LguI GCTCTTC 1 cut(s) 202
LmnI GCTCC 9 cut(s) 63, 84, 177, 188, 217, 325, 443, 778, 790
Lsp1109I GCAGC 7 cut(s) 97, 167, 340, 750, 1058, 1343, 1352
LweI GCATC 2 cut(s) 1343, 1395
MaeII ACGT 5 cut(s) 44, 281, 825, 1380, 1441
MaeIII GTNAC 8 cut(s) 395, 644, 842, 1012, 1085, 1099, 1109, 1312
MalI GATC 6 cut(s) 145, 447, 864, 891, 1188, 1298
MbiI CCGCTC 1 cut(s) 502
MboI GATC 6 cut(s) 143, 445, 862, 889, 1186, 1296
MboII GAAGA 6 cut(s) 58, 184, 219, 244, 501, 848
MfeI CAATTG 1 cut(s) 579
MflI RGATCY 4 cut(s) 143, 445, 1186, 1296
MhlI GDGCHC 3 cut(s) 214, 343, 420
MluCI AATT 9 cut(s) 429, 465, 579, 703, 765, 972, 1039, 1215, 1355
Mly113I GGCGCC 1 cut(s) 30
MlyI GAGTC 1 cut(s) 273
MmeI TCCRAC 1 cut(s) 1412
MroI TCCGGA 1 cut(s) 185
MseI TTAA 4 cut(s) 384, 780, 876, 1241
MslI CAYNNNNRTG 3 cut(s) 74, 932, 1118
MspA1I CMGCKG 3 cut(s) 217, 346, 1049
MspI CCGG 4 cut(s) 129, 186, 1172, 1365
MspR9I CCNGG 3 cut(s) 130, 443, 1172
MunI CAATTG 1 cut(s) 579
MvaI CCWGG 1 cut(s) 443
MvnI CGCG 2 cut(s) 121, 217
MwoI GCNNNNNNNGC 6 cut(s) 46, 55, 267, 328, 1337, 1340
NarI GGCGCC 1 cut(s) 30
NciI CCSGG 2 cut(s) 130, 1172
NcoI CCATGG 1 cut(s) 105
NdeII GATC 6 cut(s) 143, 445, 862, 889, 1186, 1296
NlaIII CATG 8 cut(s) 79, 109, 322, 536, 763, 819, 1034, 1398
NmeAIII GCCGAG 1 cut(s) 414
NmuCI GTSAC 1 cut(s) 644
NspI RCATGY 1 cut(s) 1034
PaeR7I CTCGAG 2 cut(s) 413, 629
PciSI GCTCTTC 1 cut(s) 202
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PfeI GAWTC 4 cut(s) 5, 189, 812, 900
PflFI GACNNNGTC 1 cut(s) 280
PleI GAGTC 1 cut(s) 272
PluTI GGCGCC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 272
PshAI GACNNNNGTC 1 cut(s) 1431
PsiI TTATAA 1 cut(s) 752
Psp124BI GAGCTC 1 cut(s) 214
Psp1406I AACGTT 1 cut(s) 1441
Psp6I CCWGG 1 cut(s) 441
PspEI GGTNACC 1 cut(s) 644
PspFI CCCAGC 4 cut(s) 123, 342, 1049, 1267
PspGI CCWGG 1 cut(s) 441
PspOMI GGGCCC 1 cut(s) 339
PspPI GGNCC 4 cut(s) 126, 339, 340, 1158
PspXI VCTCGAGB 1 cut(s) 629
PstI CTGCAG 3 cut(s) 740, 1048, 1342
PstNI CAGNNNCTG 1 cut(s) 1337
PsuI RGATCY 4 cut(s) 143, 445, 1186, 1296
PsyI GACNNNGTC 1 cut(s) 280
PvuII CAGCTG 2 cut(s) 346, 1049
RsaI GTAC 3 cut(s) 820, 1263, 1320
RsaNI GTAC 3 cut(s) 819, 1262, 1319
RseI CAYNNNNRTG 3 cut(s) 74, 932, 1118
SacI GAGCTC 1 cut(s) 214
SacII CCGCGG 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 202
SaqAI TTAA 4 cut(s) 384, 780, 876, 1241
Sau3AI GATC 6 cut(s) 143, 445, 862, 889, 1186, 1296
Sau96I GGNCC 4 cut(s) 126, 339, 340, 1158
SchI GAGTC 1 cut(s) 273
ScrFI CCNGG 3 cut(s) 130, 443, 1172
SduI GDGCHC 3 cut(s) 214, 343, 420
SfaNI GCATC 2 cut(s) 1343, 1395
SfcI CTRYAG 3 cut(s) 736, 1044, 1338
SfiI GGCCNNNNNGGCC 1 cut(s) 46
SfoI GGCGCC 1 cut(s) 31
Sfr274I CTCGAG 2 cut(s) 413, 629
Sfr303I CCGCGG 1 cut(s) 218
SgrBI CCGCGG 1 cut(s) 218
SinI GGWCC 1 cut(s) 1158
SlaI CTCGAG 2 cut(s) 413, 629
SmiMI CAYNNNNRTG 3 cut(s) 74, 932, 1118
SmlI CTYRAG 2 cut(s) 413, 629
SmoI CTYRAG 2 cut(s) 413, 629
Sse9I AATT 9 cut(s) 429, 465, 579, 703, 765, 972, 1039, 1215, 1355
SsiI CCGC 8 cut(s) 27, 119, 153, 215, 217, 261, 293, 502
SspDI GGCGCC 1 cut(s) 29
SstI GAGCTC 1 cut(s) 214
StyD4I CCNGG 3 cut(s) 128, 441, 1170
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 6 cut(s) 238, 315, 542, 587, 721, 1318
TaiI ACGT 5 cut(s) 47, 284, 828, 1383, 1444
TaqI TCGA 6 cut(s) 245, 284, 414, 630, 1078, 1275
TaqII GACCGA 2 cut(s) 124, 176
TasI AATT 9 cut(s) 429, 465, 579, 703, 765, 972, 1039, 1215, 1355
TatI WGTACW 3 cut(s) 818, 1261, 1318
TauI GCSGC 3 cut(s) 30, 121, 296
TfiI GAWTC 4 cut(s) 5, 189, 812, 900
Tru1I TTAA 4 cut(s) 384, 780, 876, 1241
Tru9I TTAA 4 cut(s) 384, 780, 876, 1241
TscAI CASTG 2 cut(s) 553, 799
TseFI GTSAC 1 cut(s) 644
TseI GCWGC 7 cut(s) 110, 180, 328, 738, 1046, 1331, 1340
Tsp45I GTSAC 1 cut(s) 644
TspDTI ATGAA 7 cut(s) 307, 521, 677, 713, 804, 1210, 1305
TspGWI ACGGA 1 cut(s) 219
TspRI CASTG 2 cut(s) 553, 799
Tth111I GACNNNGTC 1 cut(s) 280
VpaK11BI GGWCC 1 cut(s) 1158
XapI RAATTY 3 cut(s) 1039, 1215, 1355
XceI RCATGY 1 cut(s) 1034
XhoI CTCGAG 2 cut(s) 413, 629
XmiI GTMKAC 1 cut(s) 635
ZraI GACGTC 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.