Prupe.4G271300_v2.0.a1

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0016048
Physical Location & Seq
Reverse (-)
22089225 .. 22094365
5141 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G271300.1

Sequence Viewer

Length: 1446 bp
ATGGATGCAGAAAAACCCAGCATACTGGTTAGCTCCATCAAACCCACATGGGCATCTCTGCTCCTCCTCTTCACCATCATGCTTGTCCTTTCCTACCAAACATTCTCAACCATGGCGGGTCTTCCCACATGGGTCGGGTCGATTTGGCCCGCCGCAGGATCCGATACCCGTTCGGATCATCACCCGAGCACCTGTTCCGGGTTCTTCGGGGGCGTCCCCAAGAGGAAGGTGGTGATGTCGATCGAGGCCTTCGGCGGGGTCGGCGACGGGGCCACCTCCAACACCGAAGCTTTTCGGAGGGCGATTCTCCACATGCAGCGTTTCGGGGAGAGTGGTGGGGCCCAGTTGAACGTTCCTAAGGGGAGATGGTTGACGGGCAGTTTTAACCTCACCAGTAATTTCACGCTTTTTCTTGAAGAGGGCGCTGTAATTCTGGGATCCCAGGATCCGAAGCAATGGCCTATTATTGAGCCATTGCCTTCTTATGGAAGAGGAAGAGAGAGGTTAGGAGGAAGACATATAAGCCTTATTCATGGGGATGGTCTTACAAATGTTGTCATTACAGGTAATAATGGGACAATTGACGGGCAGGGGAAAATGTGGTGGGAACTATGGTGGAACAGAACACTGGAGCATACCAGAGGTCACCTGCTTGAGCTAATGAACTCTCAAAACATTCTCATCTCTAACCTCACTTTCCTCAATTCTCCATTTTGGACCATCCATCCTGTTTACTGCAGTAACGTTGTTATCAAGGACATGACAATCTTGGCTCCTCTGAATGCTCCTAATACTGATGGCATAGACCCAGATTCGAGCACTAATGTGTGCATTGAGGACTGTTACATTGAGAGTGGCGACGATCTTGTAGCAGTGAAAAGTGGTTGGGATCACTATGGAATCAAAATGGCTCGTCCAAGCTCAAACATCATAGTAAGAAGAGTTCATGGCACCACTCCAACTTGCTCTGGAGTGGGAATAGGTAGTGAGATGTCTGGTGGGATTTCAAATGTGACCATTGAGGATTTGCATGTTTGGGACTCTGCAGCTGGGGTGCGCATAAAAACGGACAAAGGTAGAGGGGGCTACATAGCAAATATTAGTATAAGTAACATACTGATGGAAAGGGTGAAGGCACCCATAATGTTTAGTAGAGGGGCCAATGACCACCCAGACAAAGGATGGGATCCCAAAGCTGTTCCCCAAGTAAAGGGAATTTTCATTAGCAACGTGTTCAGTTTGAATTCAACAAAAGCCCCAGAATTGAGGGGCATTGAGGGTGCTTCATTTGAAAGGATATGCTTTAAAAATGTCACAGTACTTGGTCTTGCTCCATCTGCAGCGTGGCATTGTGAATTTGTTTCAGGTTTTGCCAATGGGGTGTTTCCAGCTGCATGTCCTCAGTTGCAGGATATGGACGCTTTTGACTGGTGTCTGCAACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

482

Amino Acids

52.39

Weight (kDa)

6.36

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33440
fragaria_vesca FvH4_1g24350
malus_domestica MD15G1441700.v1.1
prunus_persica Prupe.4G271300_v2.0.a1
pyrus_communis pycom15g38970
rosa_chinensis RchiOBHm_Chr2g0120901
rosa_laevigata RLG00000018600
rosa_multiflora Rmu_sc0000621.1_g000016
rosa_roxburghii Rroxscaffold_2G00122750
rosa_rugosa Rorug02G0232600
rosa_samantha Rh2AG289000 Rh2BG300000 Rh2CG278000 Rh2DG314000
rosa_wichuraiana Rw2G023150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 657
Acc16I TGCGCA 1 cut(s) 1058
Acc36I ACCTGC 1 cut(s) 657
AccB1I GGYRCC 2 cut(s) 950, 1135
AciI CCGC 4 cut(s) 116, 150, 153, 255
AclI AACGTT 3 cut(s) 351, 744, 1441
AcsI RAATTY 3 cut(s) 1215, 1243, 1355
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 1 cut(s) 1320
AfiI CCNNNNNNNGG 6 cut(s) 155, 198, 255, 485, 1178, 1210
AflIII ACRYGT 1 cut(s) 1230
AgsI TTSAA 6 cut(s) 349, 416, 1008, 1243, 1248, 1292
AjnI CCWGG 1 cut(s) 441
AleI CACNNNNGTG 1 cut(s) 824
AluBI AGCT 7 cut(s) 33, 290, 658, 921, 1049, 1196, 1391
AluI AGCT 7 cut(s) 33, 290, 658, 921, 1049, 1196, 1391
Alw21I GWGCWC 2 cut(s) 191, 821
Ama87I CYCGRG 1 cut(s) 184
AoxI GGCC 6 cut(s) 146, 246, 270, 339, 458, 1158
ApaI GGGCCC 1 cut(s) 343
ApeKI GCWGC 4 cut(s) 316, 1046, 1340, 1391
ApoI RAATTY 3 cut(s) 1215, 1243, 1355
Asp700I GAANNNNTTC 1 cut(s) 291
AspLEI GCGC 2 cut(s) 425, 1059
AspS9I GGNCC 6 cut(s) 147, 270, 339, 340, 717, 1158
AsuC2I CCSGG 1 cut(s) 199
AsuHPI GGTGA 6 cut(s) 64, 173, 244, 382, 638, 1141
AvaI CYCGRG 1 cut(s) 184
AvaII GGWCC 1 cut(s) 717
AxyI CCTNAGG 1 cut(s) 357
BaeGI GKGCMC 1 cut(s) 343
BamHI GGATCC 4 cut(s) 158, 437, 445, 1186
BanI GGYRCC 2 cut(s) 950, 1135
BanII GRGCYC 1 cut(s) 343
BbsI GAAGAC 2 cut(s) 113, 520
Bbv12I GWGCWC 2 cut(s) 191, 821
BbvI GCAGC 4 cut(s) 328, 1058, 1352, 1378
BciT130I CCWGG 1 cut(s) 443
BcnI CCSGG 1 cut(s) 199
BfmI CTRYAG 3 cut(s) 736, 1044, 1338
BfoI RGCGCY 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 657
BisI GCNGC 5 cut(s) 153, 317, 1047, 1341, 1392
BlsI GCNGC 5 cut(s) 154, 318, 1048, 1342, 1393
BmcAI AGTACT 1 cut(s) 1320
Bme1390I CCNGG 2 cut(s) 199, 443
Bme18I GGWCC 1 cut(s) 717
BmeT110I CYCGRG 1 cut(s) 184
BmgT120I GGNCC 6 cut(s) 147, 270, 339, 340, 717, 1158
BmrFI CCNGG 2 cut(s) 199, 443
BmrI ACTGGG 1 cut(s) 337
BmsI GCATC 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 337
BoxI GACNNNNGTC 1 cut(s) 1431
BpiI GAAGAC 2 cut(s) 113, 520
BpmI CTGGAG 2 cut(s) 650, 990
BpuEI CTTGAG 1 cut(s) 674
BpuMI CCSGG 1 cut(s) 199
BsaBI GATNNNNATC 1 cut(s) 239
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 2 cut(s) 111, 441
Bsc4I CCNNNNNNNGG 6 cut(s) 155, 198, 255, 485, 1178, 1210
Bse1I ACTGG 5 cut(s) 30, 343, 393, 633, 1433
Bse21I CCTNAGG 1 cut(s) 357
Bse3DI GCAATG 2 cut(s) 461, 473
Bse8I GATNNNNATC 1 cut(s) 239
BseBI CCWGG 1 cut(s) 443
BseDI CCNNGG 2 cut(s) 111, 441
BseGI GGATG 5 cut(s) 10, 544, 720, 724, 1187
BseJI GATNNNNATC 1 cut(s) 239
BseLI CCNNNNNNNGG 6 cut(s) 155, 198, 255, 485, 1178, 1210
BseMI GCAATG 2 cut(s) 461, 473
BseMII CTCAG 1 cut(s) 1415
BseNI ACTGG 5 cut(s) 30, 343, 393, 633, 1433
BseRI GAGGAG 3 cut(s) 53, 56, 765
BseSI GKGCMC 1 cut(s) 343
BseXI GCAGC 4 cut(s) 328, 1058, 1352, 1378
BseYI CCCAGC 2 cut(s) 17, 1049
Bsh1285I CGRYCG 1 cut(s) 243
BshFI GGCC 6 cut(s) 148, 248, 272, 341, 460, 1160
BshNI GGYRCC 2 cut(s) 950, 1135
BsiEI CGRYCG 1 cut(s) 243
BsiHKAI GWGCWC 2 cut(s) 191, 821
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 1 cut(s) 198
BslFI GGGAC 3 cut(s) 200, 589, 1052
BslI CCNNNNNNNGG 6 cut(s) 155, 198, 255, 485, 1178, 1210
BsmFI GGGAC 3 cut(s) 200, 589, 1052
BsmI GAATGC 1 cut(s) 787
BsnI GGCC 6 cut(s) 148, 248, 272, 341, 460, 1160
BsoBI CYCGRG 1 cut(s) 184
Bsp120I GGGCCC 1 cut(s) 339
Bsp1286I GDGCHC 3 cut(s) 191, 343, 821
Bsp143I GATC 8 cut(s) 158, 175, 240, 437, 445, 862, 889, 1186
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 4 cut(s) 116, 150, 153, 255
BspANI GGCC 6 cut(s) 148, 248, 272, 341, 460, 1160
BspCNI CTCAG 1 cut(s) 1414
BspMAI CTGCAG 3 cut(s) 740, 1048, 1342
BspMI ACCTGC 1 cut(s) 657
BspT107I GGYRCC 2 cut(s) 950, 1135
BsrDI GCAATG 2 cut(s) 461, 473
BsrI ACTGG 5 cut(s) 30, 343, 393, 633, 1433
BssECI CCNNGG 2 cut(s) 111, 441
BssMI GATC 8 cut(s) 158, 175, 240, 437, 445, 862, 889, 1186
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 443
Bst4CI ACNGT 2 cut(s) 842, 1318
Bst6I CTCTTC 5 cut(s) 74, 411, 484, 490, 934
BstACI GRCGYC 1 cut(s) 213
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 2 cut(s) 357, 1401
BstDSI CCRYGG 1 cut(s) 111
BstEII GGTNACC 1 cut(s) 644
BstF5I GGATG 5 cut(s) 10, 544, 720, 724, 1187
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 2 cut(s) 425, 1059
BstKTI GATC 8 cut(s) 161, 178, 243, 440, 448, 865, 892, 1189
BstMBI GATC 8 cut(s) 158, 175, 240, 437, 445, 862, 889, 1186
BstMCI CGRYCG 1 cut(s) 243
BstMWI GCNNNNNNNGC 2 cut(s) 261, 1337
BstNI CCWGG 1 cut(s) 443
BstNSI RCATGY 3 cut(s) 316, 1034, 1398
BstPAI GACNNNNGTC 1 cut(s) 1431
BstPI GGTNACC 1 cut(s) 644
BstSCI CCNGG 2 cut(s) 197, 441
BstSFI CTRYAG 3 cut(s) 736, 1044, 1338
BstSLI GKGCMC 1 cut(s) 343
BstV1I GCAGC 4 cut(s) 328, 1058, 1352, 1378
BstV2I GAAGAC 2 cut(s) 113, 520
BstX2I RGATCY 4 cut(s) 158, 437, 445, 1186
BstXI CCANNNNNNTGG 1 cut(s) 25
BstYI RGATCY 4 cut(s) 158, 437, 445, 1186
Bsu36I CCTNAGG 1 cut(s) 357
BsuRI GGCC 6 cut(s) 148, 248, 272, 341, 460, 1160
BtgI CCRYGG 1 cut(s) 111
BtsCI GGATG 5 cut(s) 10, 544, 720, 724, 1187
BtsI GCAGTG 1 cut(s) 879
BtsIMutI CAGTG 2 cut(s) 626, 879
BveI ACCTGC 1 cut(s) 657
Cac8I GCNNGC 1 cut(s) 150
CfoI GCGC 2 cut(s) 425, 1059
Cfr13I GGNCC 6 cut(s) 147, 270, 339, 340, 717, 1158
CseI GACGC 2 cut(s) 202, 1427
Csp6I GTAC 1 cut(s) 1319
CviQI GTAC 1 cut(s) 1319
DdeI CTNAG 2 cut(s) 357, 1401
DpnI GATC 8 cut(s) 160, 177, 242, 439, 447, 864, 891, 1188
DpnII GATC 8 cut(s) 158, 175, 240, 437, 445, 862, 889, 1186
DraI TTTAAA 1 cut(s) 1306
Eam1104I CTCTTC 5 cut(s) 74, 411, 484, 490, 934
EarI CTCTTC 5 cut(s) 74, 411, 484, 490, 934
Eco130I CCWWGG 1 cut(s) 111
Eco147I AGGCCT 1 cut(s) 248
Eco24I GRGCYC 1 cut(s) 343
Eco47I GGWCC 1 cut(s) 717
Eco81I CCTNAGG 1 cut(s) 357
Eco88I CYCGRG 1 cut(s) 184
Eco91I GGTNACC 1 cut(s) 644
EcoO109I RGGNCCY 1 cut(s) 339
EcoO65I GGTNACC 1 cut(s) 644
EcoRI GAATTC 1 cut(s) 1243
EcoRII CCWGG 1 cut(s) 441
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 111
FaqI GGGAC 3 cut(s) 200, 589, 1052
FauI CCCGC 3 cut(s) 109, 157, 248
Fnu4HI GCNGC 5 cut(s) 153, 317, 1047, 1341, 1392
FokI GGATG 5 cut(s) 17, 551, 707, 711, 1194
FriOI GRGCYC 1 cut(s) 343
Fsp4HI GCNGC 5 cut(s) 153, 317, 1047, 1341, 1392
FspAI RTGCGCAY 1 cut(s) 1058
FspI TGCGCA 1 cut(s) 1058
GlaI GCGC 2 cut(s) 424, 1058
GluI GCNGC 5 cut(s) 153, 317, 1047, 1341, 1392
GsaI CCCAGC 2 cut(s) 21, 1053
GsuI CTGGAG 2 cut(s) 650, 990
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 6 cut(s) 148, 248, 272, 341, 460, 1160
HapII CCGG 1 cut(s) 198
HgaI GACGC 2 cut(s) 202, 1427
HhaI GCGC 2 cut(s) 425, 1059
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 2 cut(s) 423, 1057
HinP1I GCGC 2 cut(s) 423, 1057
HincII GTYRAC 1 cut(s) 372
HindII GTYRAC 1 cut(s) 372
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 4 cut(s) 304, 812, 900, 1040
HpaII CCGG 1 cut(s) 198
HphI GGTGA 6 cut(s) 64, 173, 244, 382, 638, 1141
Hpy166II GTNNAC 2 cut(s) 372, 733
Hpy188I TCNGA 5 cut(s) 163, 175, 297, 450, 780
Hpy188III TCNNGA 2 cut(s) 413, 969
Hpy8I GTNNAC 2 cut(s) 372, 733
Hpy99I CGWCG 2 cut(s) 269, 863
HpyAV CCTTC 4 cut(s) 220, 259, 489, 1126
HpyCH4III ACNGT 2 cut(s) 842, 1318
HpyCH4IV ACGT 4 cut(s) 351, 744, 1230, 1441
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 1337
HpyF3I CTNAG 2 cut(s) 357, 1401
HpySE526I ACGT 4 cut(s) 351, 744, 1230, 1441
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 2 cut(s) 423, 1057
Kzo9I GATC 8 cut(s) 158, 175, 240, 437, 445, 862, 889, 1186
LmnI GCTCC 6 cut(s) 38, 66, 631, 778, 790, 1336
Lsp1109I GCAGC 4 cut(s) 328, 1058, 1352, 1378
LweI GCATC 1 cut(s) 62
MaeII ACGT 4 cut(s) 351, 744, 1230, 1441
MaeIII GTNAC 6 cut(s) 644, 740, 842, 1012, 1109, 1312
MalI GATC 8 cut(s) 160, 177, 242, 439, 447, 864, 891, 1188
MboI GATC 8 cut(s) 158, 175, 240, 437, 445, 862, 889, 1186
MboII GAAGA 8 cut(s) 61, 113, 196, 428, 501, 507, 525, 951
MfeI CAATTG 1 cut(s) 579
MflI RGATCY 4 cut(s) 158, 437, 445, 1186
MhlI GDGCHC 3 cut(s) 191, 343, 821
MluCI AATT 8 cut(s) 397, 429, 579, 703, 1215, 1243, 1262, 1355
MlyI GAGTC 1 cut(s) 1034
MmeI TCCRAC 2 cut(s) 303, 983
MroXI GAANNNNTTC 1 cut(s) 291
MseI TTAA 2 cut(s) 384, 1305
MslI CAYNNNNRTG 4 cut(s) 77, 537, 824, 1118
MspA1I CMGCKG 2 cut(s) 1049, 1391
MspI CCGG 1 cut(s) 198
MspR9I CCNGG 2 cut(s) 199, 443
MunI CAATTG 1 cut(s) 579
Mva1269I GAATGC 1 cut(s) 787
MvaI CCWGG 1 cut(s) 443
MwoI GCNNNNNNNGC 2 cut(s) 261, 1337
NciI CCSGG 1 cut(s) 199
NcoI CCATGG 1 cut(s) 111
NdeII GATC 8 cut(s) 158, 175, 240, 437, 445, 862, 889, 1186
NmuCI GTSAC 3 cut(s) 644, 1012, 1312
NsbI TGCGCA 1 cut(s) 1058
NspI RCATGY 3 cut(s) 316, 1034, 1398
OliI CACNNNNGTG 1 cut(s) 824
PaqCI CACCTGC 1 cut(s) 657
PceI AGGCCT 1 cut(s) 248
PctI GAATGC 1 cut(s) 787
PdmI GAANNNNTTC 1 cut(s) 291
PfeI GAWTC 3 cut(s) 304, 812, 900
PkrI GCNGC 5 cut(s) 154, 318, 1048, 1342, 1393
Ple19I CGATCG 1 cut(s) 243
PleI GAGTC 1 cut(s) 1034
PpsI GAGTC 1 cut(s) 1034
PshAI GACNNNNGTC 1 cut(s) 1431
Psp1406I AACGTT 3 cut(s) 351, 744, 1441
Psp6I CCWGG 1 cut(s) 441
PspEI GGTNACC 1 cut(s) 644
PspFI CCCAGC 2 cut(s) 17, 1049
PspGI CCWGG 1 cut(s) 441
PspOMI GGGCCC 1 cut(s) 339
PspPI GGNCC 6 cut(s) 147, 270, 339, 340, 717, 1158
PsrI GAACNNNNNNTAC 2 cut(s) 927, 959
PstI CTGCAG 3 cut(s) 740, 1048, 1342
PsuI RGATCY 4 cut(s) 158, 437, 445, 1186
PvuI CGATCG 1 cut(s) 243
PvuII CAGCTG 2 cut(s) 1049, 1391
RsaI GTAC 1 cut(s) 1320
RsaNI GTAC 1 cut(s) 1319
RseI CAYNNNNRTG 4 cut(s) 77, 537, 824, 1118
SaqAI TTAA 2 cut(s) 384, 1305
SatI GCNGC 5 cut(s) 153, 317, 1047, 1341, 1392
Sau3AI GATC 8 cut(s) 158, 175, 240, 437, 445, 862, 889, 1186
Sau96I GGNCC 6 cut(s) 147, 270, 339, 340, 717, 1158
ScaI AGTACT 1 cut(s) 1320
SchI GAGTC 1 cut(s) 1034
ScrFI CCNGG 2 cut(s) 199, 443
SduI GDGCHC 3 cut(s) 191, 343, 821
SfaNI GCATC 1 cut(s) 62
SfcI CTRYAG 3 cut(s) 736, 1044, 1338
SinI GGWCC 1 cut(s) 717
SmiMI CAYNNNNRTG 4 cut(s) 77, 537, 824, 1118
SmlI CTYRAG 1 cut(s) 653
SmoI CTYRAG 1 cut(s) 653
Sse9I AATT 8 cut(s) 397, 429, 579, 703, 1215, 1243, 1262, 1355
SseBI AGGCCT 1 cut(s) 248
SsiI CCGC 4 cut(s) 116, 150, 153, 255
SspI AATATT 1 cut(s) 1099
StuI AGGCCT 1 cut(s) 248
StyD4I CCNGG 2 cut(s) 197, 441
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 2 cut(s) 842, 1318
TaiI ACGT 4 cut(s) 354, 747, 1233, 1444
TaqI TCGA 4 cut(s) 140, 239, 243, 815
TasI AATT 8 cut(s) 397, 429, 579, 703, 1215, 1243, 1262, 1355
TatI WGTACW 1 cut(s) 1318
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 3 cut(s) 304, 812, 900
Tru1I TTAA 2 cut(s) 384, 1305
Tru9I TTAA 2 cut(s) 384, 1305
TscAI CASTG 2 cut(s) 633, 879
TseFI GTSAC 3 cut(s) 644, 1012, 1312
TseI GCWGC 4 cut(s) 316, 1046, 1340, 1391
Tsp45I GTSAC 3 cut(s) 644, 1012, 1312
TspDTI ATGAA 5 cut(s) 521, 677, 935, 1210, 1275
TspGWI ACGGA 1 cut(s) 1082
TspRI CASTG 2 cut(s) 633, 879
VpaK11BI GGWCC 1 cut(s) 717
XapI RAATTY 3 cut(s) 1215, 1243, 1355
XceI RCATGY 3 cut(s) 316, 1034, 1398
XcmI CCANNNNNNNNNTGG 3 cut(s) 226, 1179, 1341
XmnI GAANNNNTTC 1 cut(s) 291
ZrmI AGTACT 1 cut(s) 1320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.