Rmu_sc0000621.1_g000016

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000621.1
OG0016048
Physical Location & Seq
Reverse (-)
67400 .. 71309
3910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000621.1_g000016.1.cds

Sequence Viewer

Length: 1278 bp
atggattccgagaagtccatgttcggcggcgccatcaggccgacgtggccgtctctgcttctcctcttcaccaccatgctcgtcctctcctaccaaatctccaccatgacgggcctccccagctgggccgggtcggtctggggagcatccgaaaccgctccggccgaccctactaggagcaggagctgctccgggttcttcttcggcggagtcggcgcggcaccgccgcggaggaagacggtgatgtcgataagagacttcggcggagtcgccgacggaacgacgtcgaatacggaagcgtttcggaaggcgatgcggtacatgcagacgaagcaggagagtggtggggcccagttgaatgttcccgaagggcgatggttgaccggcagttttaacctcaccagtaacttcacgctttttctcgaggagggcgccgtaattctcggctcccaggatccaaaggaatggcctattattgagccattgccttcttatggaagagggagagagaggttaggaggaaggcatataagccttattcatggggatggtctcacagatgttgtcattacaggtcataatggaacaattgacggtcaagggaaaatgtggtgggaactatggtggaacagaacgcttgagtatacgagaggtcaccttcttgaactaatgaactctaacaacattctcatctctaacctcaccttcctcaattctcctttttggacagttcatcctgtttactgcagcaatgtcgttataaaggacatgacaatcttggctccccttaatgctcctaacactgacggcatagacgcagctttgggatcccatcctttgtccgggtggtcattggaccctctactaaactttatgggtgtcttgaccctttccaaatctgataagggtcgagggggttacatagagaatgttactataagtaacataaagatggaaagggtcaagacacccataaagtttagtagagggtccaatgaccacccggacaaaggatgggatcccaaagctgttcccaaagtaaagggtattttcattagcaacattgtcagcttaaactcaacaaaagctccagtactgcagggtgtcgagggtgcttcctatgaagggatatgcctcaggaatgtaacgatacttggtctagcagcatctgcagcgtggcaatgtgaatttgttgccggttttgctaatgacgtatttccaacgccatgtcctcagatgcaggatatgggcttttttgactggtgtcagcagcgttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

425

Amino Acids

46.53

Weight (kDa)

7.05

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33440
fragaria_vesca FvH4_1g24350
malus_domestica MD15G1441700.v1.1
prunus_persica Prupe.4G271300_v2.0.a1
pyrus_communis pycom15g38970
rosa_chinensis RchiOBHm_Chr2g0120901
rosa_laevigata RLG00000018600
rosa_multiflora Rmu_sc0000621.1_g000016
rosa_roxburghii Rroxscaffold_2G00122750
rosa_rugosa Rorug02G0232600
rosa_samantha Rh2AG289000 Rh2BG300000 Rh2CG278000 Rh2DG314000
rosa_wichuraiana Rw2G023150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 761
AasI GACNNNNNNGTC 2 cut(s) 49, 244
AatII GACGTC 1 cut(s) 287
AccB1I GGYRCC 3 cut(s) 29, 220, 431
AccBSI CCGCTC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 644
AccII CGCG 2 cut(s) 218, 229
AciI CCGC 9 cut(s) 27, 156, 207, 218, 224, 227, 229, 264, 316
AclWI GGATC 6 cut(s) 449, 462, 822, 835, 1013, 1026
AcoI YGGCCR 2 cut(s) 47, 162
AcsI RAATTY 1 cut(s) 1187
AcyI GRCGYC 3 cut(s) 30, 284, 432
AfaI GTAC 2 cut(s) 320, 1095
AfiI CCNNNNNNNGG 5 cut(s) 124, 494, 842, 1010, 1125
AgsI TTSAA 2 cut(s) 358, 665
AjiI CACGTC 1 cut(s) 45
AjnI CCWGG 1 cut(s) 450
AluBI AGCT 6 cut(s) 123, 186, 821, 1028, 1071, 1088
AluI AGCT 6 cut(s) 123, 186, 821, 1028, 1071, 1088
Alw26I GTCTC 3 cut(s) 57, 249, 557
AlwI GGATC 6 cut(s) 449, 462, 822, 835, 1013, 1026
AlwNI CAGNNNCTG 2 cut(s) 186, 1169
Ama87I CYCGRG 1 cut(s) 422
AoxI GGCC 7 cut(s) 38, 47, 112, 126, 162, 348, 467
ApaI GGGCCC 1 cut(s) 352
ApeKI GCWGC 6 cut(s) 186, 747, 818, 1163, 1172, 1270
ApoI RAATTY 1 cut(s) 1187
Asp700I GAANNNNTTC 1 cut(s) 300
AspLEI GCGC 3 cut(s) 32, 218, 434
AspS9I GGNCC 6 cut(s) 112, 126, 348, 349, 856, 990
AsuC2I CCSGG 4 cut(s) 130, 193, 844, 1004
AsuHPI GGTGA 5 cut(s) 61, 253, 391, 647, 694
AvaI CYCGRG 1 cut(s) 422
AvaII GGWCC 2 cut(s) 856, 990
AxyI CCTNAGG 1 cut(s) 1136
BaeGI GKGCMC 1 cut(s) 352
BamHI GGATCC 3 cut(s) 454, 827, 1018
BanI GGYRCC 3 cut(s) 29, 220, 431
BanII GRGCYC 1 cut(s) 352
BbsI GAAGAC 1 cut(s) 242
BbvI GCAGC 5 cut(s) 173, 759, 830, 1175, 1184
BccI CCATC 6 cut(s) 41, 369, 542, 840, 946, 1008
BceAI ACGGC 3 cut(s) 34, 419, 823
BcgI CGANNNNNNTGC 2 cut(s) 736, 770
BciT130I CCWGG 1 cut(s) 452
BcnI CCSGG 4 cut(s) 130, 193, 844, 1004
BcoDI GTCTC 3 cut(s) 57, 249, 557
BfaI CTAG 2 cut(s) 174, 1160
BfmI CTRYAG 3 cut(s) 745, 1097, 1170
BfoI RGCGCY 2 cut(s) 33, 435
BglI GCCNNNNNGGC 1 cut(s) 46
BisI GCNGC 9 cut(s) 28, 187, 219, 227, 748, 819, 1164, 1173, 1271
BlsI GCNGC 9 cut(s) 29, 188, 220, 228, 749, 820, 1165, 1174, 1272
BmcAI AGTACT 1 cut(s) 1095
Bme1390I CCNGG 5 cut(s) 130, 193, 452, 844, 1004
Bme18I GGWCC 2 cut(s) 856, 990
BmeT110I CYCGRG 1 cut(s) 422
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 6 cut(s) 112, 126, 348, 349, 856, 990
BmrFI CCNGG 5 cut(s) 130, 193, 452, 844, 1004
BmrI ACTGGG 1 cut(s) 346
BmsI GCATC 4 cut(s) 155, 303, 1175, 1227
BmuI ACTGGG 1 cut(s) 346
BoxI GACNNNNGTC 1 cut(s) 1263
BpiI GAAGAC 1 cut(s) 242
BpmI CTGGAG 1 cut(s) 1074
BpuEI CTTGAG 1 cut(s) 659
BpuMI CCSGG 4 cut(s) 130, 193, 844, 1004
BsaHI GRCGYC 3 cut(s) 30, 284, 432
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 2 cut(s) 227, 450
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 1072, 1102
Bsc4I CCNNNNNNNGG 5 cut(s) 124, 494, 842, 1010, 1125
Bse118I RCCGGY 2 cut(s) 383, 1196
Bse1I ACTGG 4 cut(s) 352, 402, 1091, 1265
Bse21I CCTNAGG 1 cut(s) 1136
Bse3DI GCAATG 3 cut(s) 482, 757, 1187
BseBI CCWGG 1 cut(s) 452
BseDI CCNNGG 2 cut(s) 227, 450
BseGI GGATG 5 cut(s) 146, 553, 733, 832, 1019
BseLI CCNNNNNNNGG 5 cut(s) 124, 494, 842, 1010, 1125
BseMI GCAATG 3 cut(s) 482, 757, 1187
BseMII CTCAG 2 cut(s) 1150, 1247
BseNI ACTGG 4 cut(s) 352, 402, 1091, 1265
BseRI GAGGAG 2 cut(s) 53, 440
BseSI GKGCMC 1 cut(s) 352
BseX3I CGGCCG 1 cut(s) 162
BseXI GCAGC 5 cut(s) 173, 759, 830, 1175, 1184
BseYI CCCAGC 2 cut(s) 119, 123
Bsh1236I CGCG 2 cut(s) 218, 229
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
BshNI GGYRCC 3 cut(s) 29, 220, 431
BsiEI CGRYCG 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 422
BsiSI CCGG 7 cut(s) 129, 161, 192, 384, 843, 1004, 1197
BslI CCNNNNNNNGG 5 cut(s) 124, 494, 842, 1010, 1125
BsmAI GTCTC 3 cut(s) 57, 249, 557
BsmBI CGTCTC 1 cut(s) 57
BsnI GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
Bso31I GGTCTC 1 cut(s) 557
BsoBI CYCGRG 1 cut(s) 422
Bsp120I GGGCCC 1 cut(s) 348
Bsp1286I GDGCHC 1 cut(s) 352
Bsp143I GATC 3 cut(s) 454, 827, 1018
BspACI CCGC 9 cut(s) 27, 156, 207, 218, 224, 227, 229, 264, 316
BspANI GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
BspCNI CTCAG 2 cut(s) 1149, 1246
BspFNI CGCG 2 cut(s) 218, 229
BspMAI CTGCAG 3 cut(s) 749, 1101, 1174
BspPI GGATC 6 cut(s) 449, 462, 822, 835, 1013, 1026
BspT107I GGYRCC 3 cut(s) 29, 220, 431
BspTNI GGTCTC 1 cut(s) 557
BsrBI CCGCTC 1 cut(s) 158
BsrDI GCAATG 3 cut(s) 482, 757, 1187
BsrFI RCCGGY 2 cut(s) 383, 1196
BsrI ACTGG 4 cut(s) 352, 402, 1091, 1265
BssAI RCCGGY 2 cut(s) 383, 1196
BssECI CCNNGG 2 cut(s) 227, 450
BssMI GATC 3 cut(s) 454, 827, 1018
BssNAI GTATAC 1 cut(s) 645
BssNI GRCGYC 3 cut(s) 30, 284, 432
Bst1107I GTATAC 1 cut(s) 645
Bst2UI CCWGG 1 cut(s) 452
Bst4CI ACNGT 3 cut(s) 241, 596, 730
Bst6I CTCTTC 2 cut(s) 71, 493
BstACI GRCGYC 3 cut(s) 30, 284, 432
BstAPI GCANNNNNTGC 2 cut(s) 186, 1169
BstDEI CTNAG 2 cut(s) 1136, 1233
BstDSI CCRYGG 1 cut(s) 227
BstEII GGTNACC 1 cut(s) 653
BstF5I GGATG 5 cut(s) 146, 553, 733, 832, 1019
BstFNI CGCG 2 cut(s) 218, 229
BstH2I RGCGCY 2 cut(s) 33, 435
BstHHI GCGC 3 cut(s) 32, 218, 434
BstKTI GATC 3 cut(s) 457, 830, 1021
BstMAI GTCTC 3 cut(s) 57, 249, 557
BstMBI GATC 3 cut(s) 454, 827, 1018
BstMCI CGRYCG 1 cut(s) 165
BstNI CCWGG 1 cut(s) 452
BstNSI RCATGY 1 cut(s) 325
BstPAI GACNNNNGTC 1 cut(s) 1263
BstPI GGTNACC 1 cut(s) 653
BstSCI CCNGG 5 cut(s) 128, 191, 450, 842, 1002
BstSFI CTRYAG 3 cut(s) 745, 1097, 1170
BstSLI GKGCMC 1 cut(s) 352
BstUI CGCG 2 cut(s) 218, 229
BstV1I GCAGC 5 cut(s) 173, 759, 830, 1175, 1184
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 3 cut(s) 454, 827, 1018
BstXI CCANNNNNNTGG 1 cut(s) 465
BstYI RGATCY 3 cut(s) 454, 827, 1018
BstZ17I GTATAC 1 cut(s) 645
BstZI CGGCCG 1 cut(s) 162
Bsu36I CCTNAGG 1 cut(s) 1136
BsuRI GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
BtgI CCRYGG 1 cut(s) 227
BtgZI GCGATG 2 cut(s) 326, 388
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 5 cut(s) 146, 553, 733, 832, 1019
BtsIMutI CAGTG 1 cut(s) 801
CaiI CAGNNNCTG 2 cut(s) 186, 1169
CfoI GCGC 3 cut(s) 32, 218, 434
Cfr10I RCCGGY 2 cut(s) 383, 1196
Cfr13I GGNCC 6 cut(s) 112, 126, 348, 349, 856, 990
Cfr42I CCGCGG 1 cut(s) 230
CseI GACGC 1 cut(s) 824
Csp6I GTAC 2 cut(s) 319, 1094
CviAII CATG 7 cut(s) 19, 76, 106, 322, 542, 769, 1227
CviQI GTAC 2 cut(s) 319, 1094
DdeI CTNAG 2 cut(s) 1136, 1233
DinI GGCGCC 2 cut(s) 31, 433
DpnI GATC 3 cut(s) 456, 829, 1020
DpnII GATC 3 cut(s) 454, 827, 1018
DrdI GACNNNNNNGTC 2 cut(s) 49, 244
DseDI GACNNNNNNGTC 2 cut(s) 49, 244
EaeI YGGCCR 2 cut(s) 47, 162
EagI CGGCCG 1 cut(s) 162
Eam1104I CTCTTC 2 cut(s) 71, 493
EarI CTCTTC 2 cut(s) 71, 493
EciI GGCGGA 2 cut(s) 222, 279
EclXI CGGCCG 1 cut(s) 162
Eco24I GRGCYC 1 cut(s) 352
Eco31I GGTCTC 1 cut(s) 557
Eco47I GGWCC 2 cut(s) 856, 990
Eco52I CGGCCG 1 cut(s) 162
Eco81I CCTNAGG 1 cut(s) 1136
Eco88I CYCGRG 1 cut(s) 422
Eco91I GGTNACC 1 cut(s) 653
EcoO109I RGGNCCY 1 cut(s) 348
EcoO65I GGTNACC 1 cut(s) 653
EcoRII CCWGG 1 cut(s) 450
EcoT38I GRGCYC 1 cut(s) 352
EgeI GGCGCC 2 cut(s) 31, 433
EheI GGCGCC 2 cut(s) 31, 433
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 7 cut(s) 22, 79, 109, 325, 545, 772, 1230
FatI CATG 7 cut(s) 18, 75, 105, 321, 541, 768, 1226
FblI GTMKAC 1 cut(s) 644
Fnu4HI GCNGC 9 cut(s) 28, 187, 219, 227, 748, 819, 1164, 1173, 1271
FokI GGATG 5 cut(s) 133, 560, 720, 819, 1026
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 9 cut(s) 28, 187, 219, 227, 748, 819, 1164, 1173, 1271
FspBI CTAG 2 cut(s) 174, 1160
GlaI GCGC 3 cut(s) 31, 217, 433
GluI GCNGC 9 cut(s) 28, 187, 219, 227, 748, 819, 1164, 1173, 1271
GsaI CCCAGC 2 cut(s) 123, 127
GsuI CTGGAG 1 cut(s) 1074
HaeII RGCGCY 2 cut(s) 33, 435
HaeIII GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
HapII CCGG 7 cut(s) 129, 161, 192, 384, 843, 1004, 1197
HgaI GACGC 1 cut(s) 824
HhaI GCGC 3 cut(s) 32, 218, 434
Hin1I GRCGYC 3 cut(s) 30, 284, 432
Hin1II CATG 7 cut(s) 22, 79, 109, 325, 545, 772, 1230
Hin6I GCGC 3 cut(s) 30, 216, 432
HinP1I GCGC 3 cut(s) 30, 216, 432
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HinfI GANTC 3 cut(s) 5, 210, 267
HpaII CCGG 7 cut(s) 129, 161, 192, 384, 843, 1004, 1197
HphI GGTGA 5 cut(s) 61, 253, 391, 647, 694
Hpy166II GTNNAC 3 cut(s) 381, 645, 742
Hpy188I TCNGA 5 cut(s) 10, 151, 306, 901, 1236
Hpy188III TCNNGA 6 cut(s) 365, 422, 662, 883, 964, 1138
Hpy8I GTNNAC 3 cut(s) 381, 645, 742
Hpy99I CGWCG 4 cut(s) 46, 278, 286, 289
HpyAV CCTTC 7 cut(s) 301, 362, 498, 516, 668, 715, 1118
HpyCH4III ACNGT 3 cut(s) 241, 596, 730
HpyCH4IV ACGT 3 cut(s) 44, 284, 1212
HpyCH4V TGCA 5 cut(s) 325, 747, 1099, 1172, 1240
HpyF3I CTNAG 2 cut(s) 1136, 1233
HpySE526I ACGT 3 cut(s) 44, 284, 1212
Hsp92I GRCGYC 3 cut(s) 30, 284, 432
Hsp92II CATG 7 cut(s) 22, 79, 109, 325, 545, 772, 1230
HspAI GCGC 3 cut(s) 30, 216, 432
KasI GGCGCC 2 cut(s) 29, 431
KspI CCGCGG 1 cut(s) 230
Kzo9I GATC 3 cut(s) 454, 827, 1018
LmnI GCTCC 9 cut(s) 143, 163, 177, 183, 194, 452, 787, 799, 1093
Lsp1109I GCAGC 5 cut(s) 173, 759, 830, 1175, 1184
LweI GCATC 4 cut(s) 155, 303, 1175, 1227
MaeI CTAG 2 cut(s) 174, 1160
MaeII ACGT 3 cut(s) 44, 284, 1212
MaeIII GTNAC 6 cut(s) 404, 653, 917, 931, 941, 1144
MalI GATC 3 cut(s) 456, 829, 1020
MbiI CCGCTC 1 cut(s) 158
MboI GATC 3 cut(s) 454, 827, 1018
MboII GAAGA 5 cut(s) 58, 190, 193, 247, 510
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 3 cut(s) 454, 827, 1018
MhlI GDGCHC 1 cut(s) 352
MluCI AATT 4 cut(s) 438, 588, 712, 1187
Mly113I GGCGCC 2 cut(s) 30, 432
MlyI GAGTC 2 cut(s) 219, 276
MmeI TCCRAC 1 cut(s) 1244
MroXI GAANNNNTTC 1 cut(s) 300
MseI TTAA 3 cut(s) 393, 789, 1073
MslI CAYNNNNRTG 3 cut(s) 74, 546, 950
MspA1I CMGCKG 2 cut(s) 123, 229
MspI CCGG 7 cut(s) 129, 161, 192, 384, 843, 1004, 1197
MspR9I CCNGG 5 cut(s) 130, 193, 452, 844, 1004
MunI CAATTG 1 cut(s) 588
MvaI CCWGG 1 cut(s) 452
MvnI CGCG 2 cut(s) 218, 229
NarI GGCGCC 2 cut(s) 30, 432
NciI CCSGG 4 cut(s) 130, 193, 844, 1004
NdeII GATC 3 cut(s) 454, 827, 1018
NlaIII CATG 7 cut(s) 22, 79, 109, 325, 545, 772, 1230
NmeAIII GCCGAG 1 cut(s) 423
NmuCI GTSAC 1 cut(s) 653
NspI RCATGY 1 cut(s) 325
PaeR7I CTCGAG 1 cut(s) 422
PcsI WCGNNNNNNNCGW 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 9 cut(s) 29, 188, 220, 228, 749, 820, 1165, 1174, 1272
PleI GAGTC 2 cut(s) 218, 275
PluTI GGCGCC 2 cut(s) 33, 435
PpsI GAGTC 2 cut(s) 218, 275
PshAI GACNNNNGTC 1 cut(s) 1263
PsiI TTATAA 1 cut(s) 761
Psp6I CCWGG 1 cut(s) 450
PspEI GGTNACC 1 cut(s) 653
PspFI CCCAGC 2 cut(s) 119, 123
PspGI CCWGG 1 cut(s) 450
PspOMI GGGCCC 1 cut(s) 348
PspPI GGNCC 6 cut(s) 112, 126, 348, 349, 856, 990
PstI CTGCAG 3 cut(s) 749, 1101, 1174
PstNI CAGNNNCTG 2 cut(s) 186, 1169
PsuI RGATCY 3 cut(s) 454, 827, 1018
PvuII CAGCTG 1 cut(s) 123
RsaI GTAC 2 cut(s) 320, 1095
RsaNI GTAC 2 cut(s) 319, 1094
RseI CAYNNNNRTG 3 cut(s) 74, 546, 950
SacII CCGCGG 1 cut(s) 230
SaqAI TTAA 3 cut(s) 393, 789, 1073
SatI GCNGC 9 cut(s) 28, 187, 219, 227, 748, 819, 1164, 1173, 1271
Sau3AI GATC 3 cut(s) 454, 827, 1018
Sau96I GGNCC 6 cut(s) 112, 126, 348, 349, 856, 990
ScaI AGTACT 1 cut(s) 1095
SchI GAGTC 2 cut(s) 219, 276
ScrFI CCNGG 5 cut(s) 130, 193, 452, 844, 1004
SduI GDGCHC 1 cut(s) 352
SfaNI GCATC 4 cut(s) 155, 303, 1175, 1227
SfcI CTRYAG 3 cut(s) 745, 1097, 1170
SfiI GGCCNNNNNGGCC 1 cut(s) 46
SfoI GGCGCC 2 cut(s) 31, 433
Sfr274I CTCGAG 1 cut(s) 422
Sfr303I CCGCGG 1 cut(s) 230
SgrBI CCGCGG 1 cut(s) 230
SinI GGWCC 2 cut(s) 856, 990
SlaI CTCGAG 1 cut(s) 422
SmiMI CAYNNNNRTG 3 cut(s) 74, 546, 950
SmlI CTYRAG 2 cut(s) 422, 638
SmoI CTYRAG 2 cut(s) 422, 638
Sse9I AATT 4 cut(s) 438, 588, 712, 1187
SsiI CCGC 9 cut(s) 27, 156, 207, 218, 224, 227, 229, 264, 316
SspDI GGCGCC 2 cut(s) 29, 431
SspMI CTAG 2 cut(s) 174, 1160
StyD4I CCNGG 5 cut(s) 128, 191, 450, 842, 1002
TaaI ACNGT 3 cut(s) 241, 596, 730
TaiI ACGT 3 cut(s) 47, 287, 1215
TaqI TCGA 5 cut(s) 248, 287, 423, 910, 1107
TaqII GACCGA 1 cut(s) 124
TasI AATT 4 cut(s) 438, 588, 712, 1187
TatI WGTACW 1 cut(s) 1093
TauI GCSGC 3 cut(s) 30, 221, 229
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 3 cut(s) 393, 789, 1073
Tru9I TTAA 3 cut(s) 393, 789, 1073
TscAI CASTG 1 cut(s) 808
TseFI GTSAC 1 cut(s) 653
TseI GCWGC 6 cut(s) 186, 747, 818, 1163, 1172, 1270
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 5 cut(s) 530, 686, 722, 1042, 1137
TspGWI ACGGA 2 cut(s) 291, 308
TspRI CASTG 1 cut(s) 808
VpaK11BI GGWCC 2 cut(s) 856, 990
XapI RAATTY 1 cut(s) 1187
XceI RCATGY 1 cut(s) 325
XhoI CTCGAG 1 cut(s) 422
XmiI GTMKAC 1 cut(s) 644
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 2 cut(s) 174, 1160
ZraI GACGTC 1 cut(s) 285
ZrmI AGTACT 1 cut(s) 1095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.