Rorug02G0232600

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0016048
Physical Location & Seq
Reverse (-)
23225688 .. 23228111
2424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0232600.1

Sequence Viewer

Length: 951 bp
ATGCCGATGTGCTTCAGCTTGATCGCCTCGCGCGACTCCTGCTTCCGTTACTCTTTCTCCTCCGCCGGCCTCAAGTCCACCACCACCGATCTCGGCGAAGGCACCATCATCCACAGCTGGGTCCCCAAACACCCCTCCCCATCCAAACCCACCCTCCTCCTCATACACGGCATCGGCGCCAACGCAATGTGGCAGTGGCACGAGTTCATCACCCCTCTCACTTCCCGCTTCAACCTCTACGTCCCCGACCTCATTTTCTTCGGGGACTCCTATTCGTCCCGGAGTGAACGCTCCGAGACTTTCCAGGCCCAGTGCGTCATGAGCCTCATGGAGGCCCATGGCGTCAGGGGAAAAATGCACGTAGCCGGGATCAGCTACGGCGGGTTCGTGGCCTATAGCATGGCGGCTCAGTATGGGGAGAGGATTGAGAGGTTGGTGTTGTGTTGCGCTGGAGTTTGTGCCGAGGAGAAGGATATGGAGAAGGGGATGTTTCAGGTGAAGAGTGTGGAGGAGGCAGTAAGTATATTGCTGCCTCAGAGGGCGGCGCAGGTGAGGCAGTTGATGAAGATCTCGTTTTACAAGCCGGCGACCAATGTGCCCTCTTGTTTTCTCAACGATTTTATTGAGGTAATGTGCACAGATCACGTTCAAGAGAGGAGGGAACTTATTCAAGCATTACATAAGGACAGACAAATGTCTGATCTTCCCAAGATAAATCATCCTACACTAATAATCTGGGGGGAGCATGACCTGGTATTTCCAATGGAATTGGCACATCGATTAAAAAGGCAAATAGGTGAGAGTGCAGAACTAGTGACCATAAAGGATGCAGGACATGCTATAAATGTAGAGAAACCGAAAGAGATGCAGAAGTACATGAAGTCGTTCCTCCTTGATCCACTCCCTCAGCCTAAACAAGAAAAGCAAAGCAATGGCCACAAGGTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

316

Amino Acids

35.77

Weight (kDa)

7.2

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 49 - 285 3.5e-10 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 50 - 286 3.8e-21 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 52 - 288 1.4e-16 Alpha/beta hydrolase family
Abhydrolase_4 PF08386 237 - 300 6.9e-06 TAP-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33440
fragaria_vesca FvH4_1g24350
malus_domestica MD15G1441700.v1.1
prunus_persica Prupe.4G271300_v2.0.a1
pyrus_communis pycom15g38970
rosa_chinensis RchiOBHm_Chr2g0120901
rosa_laevigata RLG00000018600
rosa_multiflora Rmu_sc0000621.1_g000016
rosa_roxburghii Rroxscaffold_2G00122750
rosa_rugosa Rorug02G0232600
rosa_samantha Rh2AG289000 Rh2BG300000 Rh2CG278000 Rh2DG314000
rosa_wichuraiana Rw2G023150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 538
Acc36I ACCTGC 1 cut(s) 538
AccB1I GGYRCC 2 cut(s) 101, 176
AccB7I CCANNNNNTGG 1 cut(s) 943
AccII CGCG 2 cut(s) 31, 33
AciI CCGC 5 cut(s) 63, 226, 381, 404, 542
AclWI GGATC 2 cut(s) 377, 890
AcoI YGGCCR 1 cut(s) 934
AcyI GRCGYC 2 cut(s) 177, 342
AdeI CACNNNGTG 1 cut(s) 943
AfaI GTAC 1 cut(s) 875
AfiI CCNNNNNNNGG 3 cut(s) 118, 331, 943
AgsI TTSAA 3 cut(s) 232, 650, 671
AhlI ACTAGT 1 cut(s) 811
AjnI CCWGG 2 cut(s) 303, 750
AluBI AGCT 3 cut(s) 18, 117, 375
AluI AGCT 3 cut(s) 18, 117, 375
Alw21I GWGCWC 1 cut(s) 638
Alw26I GTCTC 1 cut(s) 290
Alw44I GTGCAC 1 cut(s) 634
AlwI GGATC 2 cut(s) 377, 890
AoxI GGCC 5 cut(s) 67, 306, 333, 390, 934
ApaLI GTGCAC 1 cut(s) 634
ApeKI GCWGC 1 cut(s) 529
Asp700I GAANNNNTTC 2 cut(s) 666, 884
AspLEI GCGC 4 cut(s) 33, 179, 449, 547
AspS9I GGNCC 3 cut(s) 121, 307, 334
AsuC2I CCSGG 2 cut(s) 280, 367
AsuHPI GGTGA 4 cut(s) 202, 508, 562, 809
AvaII GGWCC 1 cut(s) 121
BaeGI GKGCMC 2 cut(s) 600, 638
BalI TGGCCA 1 cut(s) 936
BanI GGYRCC 2 cut(s) 101, 176
BauI CACGAG 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 638
BbvCI CCTCAGC 1 cut(s) 906
BbvI GCAGC 1 cut(s) 516
BccI CCATC 2 cut(s) 113, 148
BceAI ACGGC 2 cut(s) 184, 394
BcgI CGANNNNNNTGC 4 cut(s) 577, 611, 847, 881
BciT130I CCWGG 2 cut(s) 305, 752
BcnI CCSGG 2 cut(s) 280, 367
BcoDI GTCTC 1 cut(s) 290
BcuI ACTAGT 1 cut(s) 811
BfaI CTAG 1 cut(s) 812
BfmI CTRYAG 1 cut(s) 394
BfoI RGCGCY 1 cut(s) 180
BfuAI ACCTGC 1 cut(s) 538
BglII AGATCT 1 cut(s) 567
BisI GCNGC 3 cut(s) 405, 530, 543
BlsI GCNGC 3 cut(s) 406, 531, 544
Bme1390I CCNGG 4 cut(s) 280, 305, 367, 752
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 3 cut(s) 121, 307, 334
BmiI GGNNCC 4 cut(s) 103, 122, 123, 178
BmrFI CCNGG 4 cut(s) 280, 305, 367, 752
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 3 cut(s) 180, 817, 855
BmuI ACTGGG 1 cut(s) 304
BoxI GACNNNNGTC 1 cut(s) 694
BpmI CTGGAG 1 cut(s) 471
Bpu10I CCTNAGC 1 cut(s) 906
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 2 cut(s) 280, 367
Bsa29I ATCGAT 1 cut(s) 778
BsaAI YACGTR 1 cut(s) 361
BsaBI GATNNNNATC 1 cut(s) 566
BsaHI GRCGYC 2 cut(s) 177, 342
BsaJI CCNNGG 2 cut(s) 337, 462
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 138, 168
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 331, 943
Bse118I RCCGGY 2 cut(s) 65, 583
Bse1I ACTGG 1 cut(s) 310
Bse3DI GCAATG 2 cut(s) 192, 937
Bse8I GATNNNNATC 1 cut(s) 566
BseBI CCWGG 2 cut(s) 305, 752
BseCI ATCGAT 1 cut(s) 778
BseDI CCNNGG 2 cut(s) 337, 462
BseGI GGATG 5 cut(s) 108, 140, 492, 718, 832
BseJI GATNNNNATC 1 cut(s) 566
BseLI CCNNNNNNNGG 3 cut(s) 118, 331, 943
BseMI GCAATG 2 cut(s) 192, 937
BseMII CTCAG 3 cut(s) 422, 548, 920
BseNI ACTGG 1 cut(s) 310
BseRI GAGGAG 6 cut(s) 49, 146, 149, 479, 524, 670
BseSI GKGCMC 2 cut(s) 600, 638
BseXI GCAGC 1 cut(s) 516
BseYI CCCAGC 1 cut(s) 117
BsgI GTGCAG 1 cut(s) 825
Bsh1236I CGCG 2 cut(s) 31, 33
BshFI GGCC 5 cut(s) 69, 308, 335, 392, 936
BshNI GGYRCC 2 cut(s) 101, 176
BshVI ATCGAT 1 cut(s) 778
BsiHKAI GWGCWC 1 cut(s) 638
BsiSI CCGG 4 cut(s) 66, 280, 366, 584
BslFI GGGAC 4 cut(s) 107, 227, 262, 278
BslI CCNNNNNNNGG 3 cut(s) 118, 331, 943
BsmAI GTCTC 1 cut(s) 290
BsmFI GGGAC 4 cut(s) 107, 227, 262, 278
BsnI GGCC 5 cut(s) 69, 308, 335, 392, 936
Bsp1286I GDGCHC 2 cut(s) 600, 638
Bsp143I GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
Bsp19I CCATGG 1 cut(s) 337
BspACI CCGC 5 cut(s) 63, 226, 381, 404, 542
BspANI GGCC 5 cut(s) 69, 308, 335, 392, 936
BspCNI CTCAG 3 cut(s) 421, 547, 919
BspDI ATCGAT 1 cut(s) 778
BspFNI CGCG 2 cut(s) 31, 33
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 4 cut(s) 103, 122, 123, 178
BspMI ACCTGC 1 cut(s) 538
BspPI GGATC 2 cut(s) 377, 890
BspT107I GGYRCC 2 cut(s) 101, 176
BsrDI GCAATG 2 cut(s) 192, 937
BsrFI RCCGGY 2 cut(s) 65, 583
BsrI ACTGG 1 cut(s) 310
BssAI RCCGGY 2 cut(s) 65, 583
BssECI CCNNGG 2 cut(s) 337, 462
BssMI GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
BssNI GRCGYC 2 cut(s) 177, 342
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 337
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 2 cut(s) 305, 752
Bst6I CTCTTC 1 cut(s) 494
BstACI GRCGYC 2 cut(s) 177, 342
BstAPI GCANNNNNTGC 1 cut(s) 836
BstBAI YACGTR 1 cut(s) 361
BstC8I GCNNGC 2 cut(s) 67, 585
BstDEI CTNAG 3 cut(s) 408, 534, 906
BstDSI CCRYGG 1 cut(s) 337
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 5 cut(s) 108, 140, 492, 718, 832
BstFNI CGCG 2 cut(s) 31, 33
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 4 cut(s) 33, 179, 449, 547
BstKTI GATC 7 cut(s) 24, 91, 372, 570, 643, 703, 898
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
BstMWI GCNNNNNNNGC 4 cut(s) 39, 321, 553, 836
BstNI CCWGG 2 cut(s) 305, 752
BstNSI RCATGY 1 cut(s) 839
BstPAI GACNNNNGTC 1 cut(s) 694
BstSCI CCNGG 4 cut(s) 278, 303, 365, 750
BstSFI CTRYAG 1 cut(s) 394
BstSLI GKGCMC 2 cut(s) 600, 638
BstUI CGCG 2 cut(s) 31, 33
BstV1I GCAGC 1 cut(s) 516
BstX2I RGATCY 1 cut(s) 567
BstYI RGATCY 1 cut(s) 567
Bsu15I ATCGAT 1 cut(s) 778
BsuRI GGCC 5 cut(s) 69, 308, 335, 392, 936
BsuTUI ATCGAT 1 cut(s) 778
BtgI CCRYGG 1 cut(s) 337
BtsCI GGATG 5 cut(s) 108, 140, 492, 718, 832
BtsI GCAGTG 1 cut(s) 200
BtsIMutI CAGTG 2 cut(s) 200, 317
BveI ACCTGC 1 cut(s) 538
Cac8I GCNNGC 2 cut(s) 67, 585
CciI TCATGA 1 cut(s) 318
CfoI GCGC 4 cut(s) 33, 179, 449, 547
Cfr10I RCCGGY 2 cut(s) 65, 583
Cfr13I GGNCC 3 cut(s) 121, 307, 334
ClaI ATCGAT 1 cut(s) 778
CseI GACGC 2 cut(s) 304, 331
CsiI ACCWGGT 1 cut(s) 750
Csp6I GTAC 1 cut(s) 874
CviAII CATG 7 cut(s) 319, 328, 338, 400, 746, 836, 877
CviQI GTAC 1 cut(s) 874
DdeI CTNAG 3 cut(s) 408, 534, 906
DinI GGCGCC 1 cut(s) 178
DpnI GATC 7 cut(s) 23, 90, 371, 569, 642, 702, 897
DpnII GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
DraIII CACNNNGTG 1 cut(s) 943
EaeI YGGCCR 1 cut(s) 934
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
EciI GGCGGA 1 cut(s) 52
Eco130I CCWWGG 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 121
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 121
EcoRII CCWGG 2 cut(s) 303, 750
EcoT14I CCWWGG 1 cut(s) 337
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 337
FaeI CATG 7 cut(s) 322, 331, 341, 403, 749, 839, 880
FaqI GGGAC 4 cut(s) 107, 227, 262, 278
FatI CATG 7 cut(s) 318, 327, 337, 399, 745, 835, 876
FauI CCCGC 2 cut(s) 233, 374
Fnu4HI GCNGC 3 cut(s) 405, 530, 543
FokI GGATG 5 cut(s) 95, 127, 499, 705, 839
Fsp4HI GCNGC 3 cut(s) 405, 530, 543
FspBI CTAG 1 cut(s) 812
GlaI GCGC 4 cut(s) 32, 178, 448, 546
GluI GCNGC 3 cut(s) 405, 530, 543
GsaI CCCAGC 1 cut(s) 121
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 5 cut(s) 69, 308, 335, 392, 936
HapII CCGG 4 cut(s) 66, 280, 366, 584
HgaI GACGC 2 cut(s) 304, 331
HhaI GCGC 4 cut(s) 33, 179, 449, 547
Hin1I GRCGYC 2 cut(s) 177, 342
Hin1II CATG 7 cut(s) 322, 331, 341, 403, 749, 839, 880
Hin6I GCGC 4 cut(s) 31, 177, 447, 545
HinP1I GCGC 4 cut(s) 31, 177, 447, 545
HinfI GANTC 2 cut(s) 35, 266
HpaII CCGG 4 cut(s) 66, 280, 366, 584
HphI GGTGA 4 cut(s) 202, 508, 562, 809
Hpy166II GTNNAC 3 cut(s) 78, 287, 636
Hpy188I TCNGA 3 cut(s) 295, 537, 700
Hpy188III TCNNGA 2 cut(s) 319, 650
Hpy8I GTNNAC 3 cut(s) 78, 287, 636
HpyAV CCTTC 3 cut(s) 92, 463, 475
HpyCH4IV ACGT 3 cut(s) 240, 360, 645
HpyCH4V TGCA 5 cut(s) 358, 636, 806, 830, 868
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 321, 553, 836
HpyF3I CTNAG 3 cut(s) 408, 534, 906
HpySE526I ACGT 3 cut(s) 240, 360, 645
Hsp92I GRCGYC 2 cut(s) 177, 342
Hsp92II CATG 7 cut(s) 322, 331, 341, 403, 749, 839, 880
HspAI GCGC 4 cut(s) 31, 177, 447, 545
KasI GGCGCC 1 cut(s) 176
KflI GGGWCCC 1 cut(s) 121
KroI GCCGGC 2 cut(s) 65, 583
KroNI GCCGGC 2 cut(s) 67, 585
Kzo9I GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
LmnI GCTCC 2 cut(s) 296, 742
Lsp1109I GCAGC 1 cut(s) 516
LweI GCATC 3 cut(s) 180, 817, 855
MabI ACCWGGT 1 cut(s) 750
MaeI CTAG 1 cut(s) 812
MaeII ACGT 3 cut(s) 240, 360, 645
MaeIII GTNAC 2 cut(s) 47, 814
MalI GATC 7 cut(s) 23, 90, 371, 569, 642, 702, 897
MboI GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
MboII GAAGA 4 cut(s) 250, 511, 577, 695
MflI RGATCY 1 cut(s) 567
MhlI GDGCHC 2 cut(s) 600, 638
MlsI TGGCCA 1 cut(s) 936
MluCI AATT 1 cut(s) 767
MluNI TGGCCA 1 cut(s) 936
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 2 cut(s) 29, 260
Mox20I TGGCCA 1 cut(s) 936
MroNI GCCGGC 2 cut(s) 65, 583
MroXI GAANNNNTTC 2 cut(s) 666, 884
MscI TGGCCA 1 cut(s) 936
MseI TTAA 1 cut(s) 782
Msp20I TGGCCA 1 cut(s) 936
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 4 cut(s) 66, 280, 366, 584
MspR9I CCNGG 4 cut(s) 280, 305, 367, 752
MvaI CCWGG 2 cut(s) 305, 752
MvnI CGCG 2 cut(s) 31, 33
MwoI GCNNNNNNNGC 4 cut(s) 39, 321, 553, 836
NaeI GCCGGC 2 cut(s) 67, 585
NarI GGCGCC 1 cut(s) 177
NciI CCSGG 2 cut(s) 280, 367
NcoI CCATGG 1 cut(s) 337
NdeII GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
NgoMIV GCCGGC 2 cut(s) 65, 583
NlaIII CATG 7 cut(s) 322, 331, 341, 403, 749, 839, 880
NlaIV GGNNCC 4 cut(s) 103, 122, 123, 178
NmeAIII GCCGAG 2 cut(s) 72, 487
NmuCI GTSAC 1 cut(s) 814
NspI RCATGY 1 cut(s) 839
PagI TCATGA 1 cut(s) 318
PaqCI CACCTGC 1 cut(s) 538
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 384
PdiI GCCGGC 2 cut(s) 67, 585
PdmI GAANNNNTTC 2 cut(s) 666, 884
PflMI CCANNNNNTGG 1 cut(s) 943
PfoI TCCNGGA 1 cut(s) 278
PkrI GCNGC 3 cut(s) 406, 531, 544
PleI GAGTC 2 cut(s) 29, 260
PluTI GGCGCC 1 cut(s) 180
PpsI GAGTC 2 cut(s) 29, 260
Ppu21I YACGTR 1 cut(s) 361
PpuMI RGGWCCY 1 cut(s) 121
PshAI GACNNNNGTC 1 cut(s) 694
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 2 cut(s) 303, 750
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 2 cut(s) 303, 750
PspN4I GGNNCC 4 cut(s) 103, 122, 123, 178
PspPI GGNCC 3 cut(s) 121, 307, 334
PspPPI RGGWCCY 1 cut(s) 121
PsuI RGATCY 1 cut(s) 567
PvuII CAGCTG 1 cut(s) 117
RsaI GTAC 1 cut(s) 875
RsaNI GTAC 1 cut(s) 874
SaqAI TTAA 1 cut(s) 782
SatI GCNGC 3 cut(s) 405, 530, 543
Sau3AI GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
Sau96I GGNCC 3 cut(s) 121, 307, 334
SchI GAGTC 2 cut(s) 29, 260
ScrFI CCNGG 4 cut(s) 280, 305, 367, 752
SduI GDGCHC 2 cut(s) 600, 638
SexAI ACCWGGT 1 cut(s) 750
SfaNI GCATC 3 cut(s) 180, 817, 855
SfcI CTRYAG 1 cut(s) 394
SfoI GGCGCC 1 cut(s) 178
SinI GGWCC 1 cut(s) 121
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SpeI ACTAGT 1 cut(s) 811
Sse9I AATT 1 cut(s) 767
SsiI CCGC 5 cut(s) 63, 226, 381, 404, 542
SspDI GGCGCC 1 cut(s) 176
SspMI CTAG 1 cut(s) 812
StyD4I CCNGG 4 cut(s) 278, 303, 365, 750
StyI CCWWGG 1 cut(s) 337
TaiI ACGT 3 cut(s) 243, 363, 648
TaqI TCGA 1 cut(s) 778
TasI AATT 1 cut(s) 767
TatI WGTACW 1 cut(s) 873
TauI GCSGC 2 cut(s) 407, 545
Tru1I TTAA 1 cut(s) 782
Tru9I TTAA 1 cut(s) 782
TscAI CASTG 2 cut(s) 200, 317
TseFI GTSAC 1 cut(s) 814
TseI GCWGC 1 cut(s) 529
Tsp45I GTSAC 1 cut(s) 814
TspDTI ATGAA 3 cut(s) 196, 578, 893
TspGWI ACGGA 1 cut(s) 35
TspRI CASTG 2 cut(s) 200, 317
Van91I CCANNNNNTGG 1 cut(s) 943
VneI GTGCAC 1 cut(s) 634
VpaK11BI GGWCC 1 cut(s) 121
XagI CCTNNNNNAGG 1 cut(s) 329
XceI RCATGY 1 cut(s) 839
XmnI GAANNNNTTC 2 cut(s) 666, 884
XspI CTAG 1 cut(s) 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.