Rw2G023150

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016048
Physical Location & Seq
Reverse (-)
33425128 .. 33428666
3539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G023150.1

Sequence Viewer

Length: 1455 bp
ATGGATTCCGAGAAATCCATGTTCGGCGGCGCCATCAGGCCGACGTGGCCGTCTCTGCTTCTCCTCTTCACCACCATGCTCGTCCTCTCCTACCAAATCTCCACCATGACGGGCCTCCCCAGCTGGGCCGGGTCGGTCTGGGGAGCATCCGAAACCGCTCCGGCCGACCCTACGCGGAGCAGGAGCTGCTCCGGGCTCTTCTTCGGCGGAGTCGGCGCGGCACCGCCGCGGAGGAAGACGGTGATGTCGATAAGAGACTTCGGCGGAGTCGCCGACGGAACGACGTCGAATACGGAAGCGTTTCGGAAGGCGATGCGGTACATGCAGACGAAGCAGGAGAGTGGTGGGGCCCAGTTGAATGTTCCCGAAGGGCGATGGTTGACCGGCAGTTTTAACCTCACCAGTAACTTCACGCTTTTTCTCGAGGAGGGCGCCGTAATTCTCGGCTCCCAGGATCCAAAGGAATGGCCTATTATTGAGCCATTGCCTTCTTATGGAAGAGGGAGAGAGAGGTTAGGAGGAAGGCATATAAGCCTTATTCATGGGGATGGTCTCACGGATGTTGTCATTACAGGTCATAATGGAACAATTGACGGTCAAGGGAAAATGTGGTGGGAACTATGGTGGAACAGAACGCTTGAGTATACGAGAGGTCACCTTCTTGAACTAATGAACTCTAACAACATTCTCATCTCTAACCTCACCTTCCTCAATTCTCCTTTTTGGACAGTTCATCCTGTTTACTGCAGCAACGTCGTTATAAAGGACATGACAATCTTGGCTCCCCTTAATGCTCCTAACACTGACGGCATAGACGCAGATTCATGTACAAATGTGTGCATTGAAGACTGTTACATTGAGAGTGGCGACGATCTTATAGCAGTTAAAAGTGGGTGGGATCATTATGGAATCAAGATGGCTCGTCCAAGCTCAAACATCATAGTGAGGAGAGTTTCTGGCACCACTCCCACCTGTTCTGGAGTTGGAATAGGTAGTGAGATGTCTGGTGGGATTTCAAATGTTACCATTGAGGATTTGCATGTTTGGAATTCTGCAGCTGGGGTGCGTTTAAAATCTGATAAGGGTCGAGGGGGTTACATAGAAAATGTTACTATAAGTAACATAAAGATGGAAAGGGTCAAGACACCCATAAAGTTTAGTAGAGGGTCCAATGACCACCCGGACAAAGGATGGGATCCCAAAGCTGTTCCCAAAGTAAAGGGTATTTTCATTAGCAACATTGTCAGCTTAAACTCAACAAAAGCTCCAGTACTGCAGGGTGTCGAGGGTGCTTCCTATGAAGGGATATGCCTCAGGAATGTAACGATACTTGGTCTAGCAGCATCTGCAGCGTGGCAATGTGAATTTGTTGCCGGTTTTGCTAATGACGTATTTCCAACGCCATGTCCTCAGATGCAGGATATGGGCTCTTTTAACTGGTGTCAGCAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

484

Amino Acids

52.67

Weight (kDa)

6.33

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 179 - 457 2.2e-42 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33440
fragaria_vesca FvH4_1g24350
malus_domestica MD15G1441700.v1.1
prunus_persica Prupe.4G271300_v2.0.a1
pyrus_communis pycom15g38970
rosa_chinensis RchiOBHm_Chr2g0120901
rosa_laevigata RLG00000018600
rosa_multiflora Rmu_sc0000621.1_g000016
rosa_roxburghii Rroxscaffold_2G00122750
rosa_rugosa Rorug02G0232600
rosa_samantha Rh2AG289000 Rh2BG300000 Rh2CG278000 Rh2DG314000
rosa_wichuraiana Rw2G023150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 761
AasI GACNNNNNNGTC 2 cut(s) 49, 244
AatII GACGTC 1 cut(s) 287
AccB1I GGYRCC 4 cut(s) 29, 220, 431, 959
AccBSI CCGCTC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 644
AccII CGCG 3 cut(s) 175, 218, 229
AclWI GGATC 5 cut(s) 449, 462, 906, 1190, 1203
AcoI YGGCCR 2 cut(s) 47, 162
AcsI RAATTY 2 cut(s) 1048, 1364
AcyI GRCGYC 3 cut(s) 30, 284, 432
AfaI GTAC 3 cut(s) 320, 829, 1272
AfiI CCNNNNNNNGG 4 cut(s) 124, 494, 1187, 1302
AgsI TTSAA 4 cut(s) 358, 665, 845, 1017
AjiI CACGTC 1 cut(s) 45
AjnI CCWGG 1 cut(s) 450
AluBI AGCT 7 cut(s) 123, 186, 930, 1058, 1205, 1248, 1265
AluI AGCT 7 cut(s) 123, 186, 930, 1058, 1205, 1248, 1265
Alw26I GTCTC 3 cut(s) 57, 249, 557
AlwI GGATC 5 cut(s) 449, 462, 906, 1190, 1203
AlwNI CAGNNNCTG 2 cut(s) 186, 1346
Ama87I CYCGRG 1 cut(s) 422
AoxI GGCC 7 cut(s) 38, 47, 112, 126, 162, 348, 467
ApaI GGGCCC 1 cut(s) 352
ApeKI GCWGC 5 cut(s) 186, 747, 1055, 1340, 1349
ApoI RAATTY 2 cut(s) 1048, 1364
Asp700I GAANNNNTTC 1 cut(s) 300
AspLEI GCGC 3 cut(s) 32, 218, 434
AspS9I GGNCC 5 cut(s) 112, 126, 348, 349, 1167
AsuC2I CCSGG 3 cut(s) 130, 193, 1181
AsuHPI GGTGA 5 cut(s) 61, 253, 391, 647, 694
AvaI CYCGRG 1 cut(s) 422
AvaII GGWCC 1 cut(s) 1167
AxyI CCTNAGG 1 cut(s) 1313
BaeGI GKGCMC 1 cut(s) 352
BamHI GGATCC 2 cut(s) 454, 1195
BanI GGYRCC 4 cut(s) 29, 220, 431, 959
BanII GRGCYC 3 cut(s) 198, 352, 1430
BbsI GAAGAC 2 cut(s) 242, 852
BbvI GCAGC 5 cut(s) 173, 759, 1067, 1352, 1361
BccI CCATC 6 cut(s) 41, 369, 542, 910, 1123, 1185
BceAI ACGGC 3 cut(s) 34, 419, 823
BcgI CGANNNNNNTGC 2 cut(s) 736, 770
BciT130I CCWGG 1 cut(s) 452
BcnI CCSGG 3 cut(s) 130, 193, 1181
BcoDI GTCTC 3 cut(s) 57, 249, 557
BfaI CTAG 1 cut(s) 1337
BfmI CTRYAG 4 cut(s) 745, 1053, 1274, 1347
BfoI RGCGCY 2 cut(s) 33, 435
BglI GCCNNNNNGGC 1 cut(s) 46
BisI GCNGC 8 cut(s) 28, 187, 219, 227, 748, 1056, 1341, 1350
BlsI GCNGC 8 cut(s) 29, 188, 220, 228, 749, 1057, 1342, 1351
BmcAI AGTACT 1 cut(s) 1272
Bme1390I CCNGG 4 cut(s) 130, 193, 452, 1181
Bme18I GGWCC 1 cut(s) 1167
BmeT110I CYCGRG 1 cut(s) 422
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 5 cut(s) 112, 126, 348, 349, 1167
BmrFI CCNGG 4 cut(s) 130, 193, 452, 1181
BmrI ACTGGG 1 cut(s) 346
BmsI GCATC 4 cut(s) 155, 303, 1352, 1404
BmuI ACTGGG 1 cut(s) 346
BpiI GAAGAC 2 cut(s) 242, 852
BpmI CTGGAG 2 cut(s) 999, 1251
BpuEI CTTGAG 1 cut(s) 659
BpuMI CCSGG 3 cut(s) 130, 193, 1181
BsaHI GRCGYC 3 cut(s) 30, 284, 432
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 2 cut(s) 227, 450
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 1249, 1279
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 494, 1187, 1302
Bse118I RCCGGY 2 cut(s) 383, 1373
Bse1I ACTGG 4 cut(s) 352, 402, 1268, 1442
Bse21I CCTNAGG 1 cut(s) 1313
Bse3DI GCAATG 2 cut(s) 482, 1364
BseBI CCWGG 1 cut(s) 452
BseDI CCNNGG 2 cut(s) 227, 450
BseGI GGATG 5 cut(s) 146, 553, 565, 733, 1196
BseLI CCNNNNNNNGG 4 cut(s) 124, 494, 1187, 1302
BseMI GCAATG 2 cut(s) 482, 1364
BseMII CTCAG 2 cut(s) 1327, 1424
BseNI ACTGG 4 cut(s) 352, 402, 1268, 1442
BseRI GAGGAG 3 cut(s) 53, 440, 961
BseSI GKGCMC 1 cut(s) 352
BseX3I CGGCCG 1 cut(s) 162
BseXI GCAGC 5 cut(s) 173, 759, 1067, 1352, 1361
BseYI CCCAGC 3 cut(s) 119, 123, 1058
Bsh1236I CGCG 3 cut(s) 175, 218, 229
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
BshNI GGYRCC 4 cut(s) 29, 220, 431, 959
BsiEI CGRYCG 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 422
BsiSI CCGG 6 cut(s) 129, 161, 192, 384, 1181, 1374
BslI CCNNNNNNNGG 4 cut(s) 124, 494, 1187, 1302
BsmAI GTCTC 3 cut(s) 57, 249, 557
BsmBI CGTCTC 1 cut(s) 57
BsnI GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
Bso31I GGTCTC 1 cut(s) 557
BsoBI CYCGRG 1 cut(s) 422
Bsp120I GGGCCC 1 cut(s) 348
Bsp1286I GDGCHC 3 cut(s) 198, 352, 1430
Bsp1407I TGTACA 1 cut(s) 827
Bsp143I GATC 4 cut(s) 454, 871, 898, 1195
BspANI GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
BspCNI CTCAG 2 cut(s) 1326, 1423
BspFNI CGCG 3 cut(s) 175, 218, 229
BspMAI CTGCAG 4 cut(s) 749, 1057, 1278, 1351
BspPI GGATC 5 cut(s) 449, 462, 906, 1190, 1203
BspQI GCTCTTC 1 cut(s) 203
BspT107I GGYRCC 4 cut(s) 29, 220, 431, 959
BspTNI GGTCTC 1 cut(s) 557
BsrBI CCGCTC 1 cut(s) 158
BsrDI GCAATG 2 cut(s) 482, 1364
BsrFI RCCGGY 2 cut(s) 383, 1373
BsrGI TGTACA 1 cut(s) 827
BsrI ACTGG 4 cut(s) 352, 402, 1268, 1442
BssAI RCCGGY 2 cut(s) 383, 1373
BssECI CCNNGG 2 cut(s) 227, 450
BssMI GATC 4 cut(s) 454, 871, 898, 1195
BssNAI GTATAC 1 cut(s) 645
BssNI GRCGYC 3 cut(s) 30, 284, 432
Bst1107I GTATAC 1 cut(s) 645
Bst2UI CCWGG 1 cut(s) 452
Bst4CI ACNGT 4 cut(s) 241, 596, 730, 851
Bst6I CTCTTC 3 cut(s) 71, 203, 493
BstACI GRCGYC 3 cut(s) 30, 284, 432
BstAPI GCANNNNNTGC 2 cut(s) 186, 1346
BstAUI TGTACA 1 cut(s) 827
BstDEI CTNAG 2 cut(s) 1313, 1410
BstDSI CCRYGG 1 cut(s) 227
BstEII GGTNACC 1 cut(s) 653
BstF5I GGATG 5 cut(s) 146, 553, 565, 733, 1196
BstFNI CGCG 3 cut(s) 175, 218, 229
BstH2I RGCGCY 2 cut(s) 33, 435
BstHHI GCGC 3 cut(s) 32, 218, 434
BstKTI GATC 4 cut(s) 457, 874, 901, 1198
BstMAI GTCTC 3 cut(s) 57, 249, 557
BstMBI GATC 4 cut(s) 454, 871, 898, 1195
BstMCI CGRYCG 1 cut(s) 165
BstNI CCWGG 1 cut(s) 452
BstNSI RCATGY 2 cut(s) 325, 1043
BstPI GGTNACC 1 cut(s) 653
BstSCI CCNGG 4 cut(s) 128, 191, 450, 1179
BstSFI CTRYAG 4 cut(s) 745, 1053, 1274, 1347
BstSLI GKGCMC 1 cut(s) 352
BstUI CGCG 3 cut(s) 175, 218, 229
BstV1I GCAGC 5 cut(s) 173, 759, 1067, 1352, 1361
BstV2I GAAGAC 2 cut(s) 242, 852
BstX2I RGATCY 2 cut(s) 454, 1195
BstXI CCANNNNNNTGG 1 cut(s) 465
BstYI RGATCY 2 cut(s) 454, 1195
BstZ17I GTATAC 1 cut(s) 645
BstZI CGGCCG 1 cut(s) 162
Bsu36I CCTNAGG 1 cut(s) 1313
BsuRI GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
BtgI CCRYGG 1 cut(s) 227
BtgZI GCGATG 2 cut(s) 326, 388
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 5 cut(s) 146, 553, 565, 733, 1196
BtsIMutI CAGTG 1 cut(s) 801
CaiI CAGNNNCTG 2 cut(s) 186, 1346
CfoI GCGC 3 cut(s) 32, 218, 434
Cfr10I RCCGGY 2 cut(s) 383, 1373
Cfr13I GGNCC 5 cut(s) 112, 126, 348, 349, 1167
Cfr42I CCGCGG 1 cut(s) 230
CseI GACGC 1 cut(s) 824
Csp6I GTAC 3 cut(s) 319, 828, 1271
CviAII CATG 9 cut(s) 19, 76, 106, 322, 542, 769, 825, 1040, 1404
CviQI GTAC 3 cut(s) 319, 828, 1271
DdeI CTNAG 2 cut(s) 1313, 1410
DinI GGCGCC 2 cut(s) 31, 433
DpnI GATC 4 cut(s) 456, 873, 900, 1197
DpnII GATC 4 cut(s) 454, 871, 898, 1195
DraI TTTAAA 1 cut(s) 1071
DrdI GACNNNNNNGTC 2 cut(s) 49, 244
DseDI GACNNNNNNGTC 2 cut(s) 49, 244
EaeI YGGCCR 2 cut(s) 47, 162
EagI CGGCCG 1 cut(s) 162
Eam1104I CTCTTC 3 cut(s) 71, 203, 493
EarI CTCTTC 3 cut(s) 71, 203, 493
EciI GGCGGA 2 cut(s) 222, 279
EclXI CGGCCG 1 cut(s) 162
Eco24I GRGCYC 3 cut(s) 198, 352, 1430
Eco31I GGTCTC 1 cut(s) 557
Eco47I GGWCC 1 cut(s) 1167
Eco52I CGGCCG 1 cut(s) 162
Eco81I CCTNAGG 1 cut(s) 1313
Eco88I CYCGRG 1 cut(s) 422
Eco91I GGTNACC 1 cut(s) 653
EcoO109I RGGNCCY 1 cut(s) 348
EcoO65I GGTNACC 1 cut(s) 653
EcoRI GAATTC 1 cut(s) 1048
EcoRII CCWGG 1 cut(s) 450
EcoT38I GRGCYC 3 cut(s) 198, 352, 1430
EgeI GGCGCC 2 cut(s) 31, 433
EheI GGCGCC 2 cut(s) 31, 433
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 9 cut(s) 22, 79, 109, 325, 545, 772, 828, 1043, 1407
FatI CATG 9 cut(s) 18, 75, 105, 321, 541, 768, 824, 1039, 1403
FblI GTMKAC 1 cut(s) 644
Fnu4HI GCNGC 8 cut(s) 28, 187, 219, 227, 748, 1056, 1341, 1350
FokI GGATG 5 cut(s) 133, 560, 572, 720, 1203
FriOI GRGCYC 3 cut(s) 198, 352, 1430
Fsp4HI GCNGC 8 cut(s) 28, 187, 219, 227, 748, 1056, 1341, 1350
FspBI CTAG 1 cut(s) 1337
GlaI GCGC 3 cut(s) 31, 217, 433
GluI GCNGC 8 cut(s) 28, 187, 219, 227, 748, 1056, 1341, 1350
GsaI CCCAGC 3 cut(s) 123, 127, 1062
GsuI CTGGAG 2 cut(s) 999, 1251
HaeII RGCGCY 2 cut(s) 33, 435
HaeIII GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
HapII CCGG 6 cut(s) 129, 161, 192, 384, 1181, 1374
HgaI GACGC 1 cut(s) 824
HhaI GCGC 3 cut(s) 32, 218, 434
Hin1I GRCGYC 3 cut(s) 30, 284, 432
Hin1II CATG 9 cut(s) 22, 79, 109, 325, 545, 772, 828, 1043, 1407
Hin6I GCGC 3 cut(s) 30, 216, 432
HinP1I GCGC 3 cut(s) 30, 216, 432
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HinfI GANTC 5 cut(s) 5, 210, 267, 821, 909
HpaII CCGG 6 cut(s) 129, 161, 192, 384, 1181, 1374
HphI GGTGA 5 cut(s) 61, 253, 391, 647, 694
Hpy166II GTNNAC 3 cut(s) 381, 645, 742
Hpy188I TCNGA 5 cut(s) 10, 151, 306, 1078, 1413
Hpy188III TCNNGA 7 cut(s) 365, 422, 662, 913, 978, 1141, 1315
Hpy8I GTNNAC 3 cut(s) 381, 645, 742
Hpy99I CGWCG 6 cut(s) 46, 278, 286, 289, 758, 872
HpyAV CCTTC 7 cut(s) 301, 362, 498, 516, 668, 715, 1295
HpyCH4III ACNGT 4 cut(s) 241, 596, 730, 851
HpyCH4IV ACGT 4 cut(s) 44, 284, 753, 1389
HpyCH4V TGCA 8 cut(s) 325, 747, 840, 1039, 1055, 1276, 1349, 1417
HpyF3I CTNAG 2 cut(s) 1313, 1410
HpySE526I ACGT 4 cut(s) 44, 284, 753, 1389
Hsp92I GRCGYC 3 cut(s) 30, 284, 432
Hsp92II CATG 9 cut(s) 22, 79, 109, 325, 545, 772, 828, 1043, 1407
HspAI GCGC 3 cut(s) 30, 216, 432
KasI GGCGCC 2 cut(s) 29, 431
KspI CCGCGG 1 cut(s) 230
Kzo9I GATC 4 cut(s) 454, 871, 898, 1195
LguI GCTCTTC 1 cut(s) 203
LmnI GCTCC 9 cut(s) 143, 163, 177, 183, 194, 452, 787, 799, 1270
Lsp1109I GCAGC 5 cut(s) 173, 759, 1067, 1352, 1361
LweI GCATC 4 cut(s) 155, 303, 1352, 1404
MaeI CTAG 1 cut(s) 1337
MaeII ACGT 4 cut(s) 44, 284, 753, 1389
MaeIII GTNAC 8 cut(s) 404, 653, 851, 1021, 1094, 1108, 1118, 1321
MalI GATC 4 cut(s) 456, 873, 900, 1197
MbiI CCGCTC 1 cut(s) 158
MboI GATC 4 cut(s) 454, 871, 898, 1195
MboII GAAGA 6 cut(s) 58, 190, 193, 247, 510, 857
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 2 cut(s) 454, 1195
MhlI GDGCHC 3 cut(s) 198, 352, 1430
MluCI AATT 5 cut(s) 438, 588, 712, 1048, 1364
Mly113I GGCGCC 2 cut(s) 30, 432
MlyI GAGTC 2 cut(s) 219, 276
MmeI TCCRAC 2 cut(s) 964, 1421
MroXI GAANNNNTTC 1 cut(s) 300
MseI TTAA 6 cut(s) 393, 789, 885, 1070, 1250, 1434
MslI CAYNNNNRTG 4 cut(s) 74, 546, 941, 1127
MspA1I CMGCKG 3 cut(s) 123, 229, 1058
MspI CCGG 6 cut(s) 129, 161, 192, 384, 1181, 1374
MspR9I CCNGG 4 cut(s) 130, 193, 452, 1181
MunI CAATTG 1 cut(s) 588
MvaI CCWGG 1 cut(s) 452
MvnI CGCG 3 cut(s) 175, 218, 229
NarI GGCGCC 2 cut(s) 30, 432
NciI CCSGG 3 cut(s) 130, 193, 1181
NdeII GATC 4 cut(s) 454, 871, 898, 1195
NlaIII CATG 9 cut(s) 22, 79, 109, 325, 545, 772, 828, 1043, 1407
NmeAIII GCCGAG 1 cut(s) 423
NmuCI GTSAC 1 cut(s) 653
NspI RCATGY 2 cut(s) 325, 1043
PaeR7I CTCGAG 1 cut(s) 422
PciSI GCTCTTC 1 cut(s) 203
PcsI WCGNNNNNNNCGW 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 3 cut(s) 5, 821, 909
PkrI GCNGC 8 cut(s) 29, 188, 220, 228, 749, 1057, 1342, 1351
PleI GAGTC 2 cut(s) 218, 275
PluTI GGCGCC 2 cut(s) 33, 435
PpsI GAGTC 2 cut(s) 218, 275
PsiI TTATAA 1 cut(s) 761
Psp6I CCWGG 1 cut(s) 450
PspEI GGTNACC 1 cut(s) 653
PspFI CCCAGC 3 cut(s) 119, 123, 1058
PspGI CCWGG 1 cut(s) 450
PspOMI GGGCCC 1 cut(s) 348
PspPI GGNCC 5 cut(s) 112, 126, 348, 349, 1167
PstI CTGCAG 4 cut(s) 749, 1057, 1278, 1351
PstNI CAGNNNCTG 2 cut(s) 186, 1346
PsuI RGATCY 2 cut(s) 454, 1195
PvuII CAGCTG 2 cut(s) 123, 1058
RsaI GTAC 3 cut(s) 320, 829, 1272
RsaNI GTAC 3 cut(s) 319, 828, 1271
RseI CAYNNNNRTG 4 cut(s) 74, 546, 941, 1127
SacII CCGCGG 1 cut(s) 230
SapI GCTCTTC 1 cut(s) 203
SaqAI TTAA 6 cut(s) 393, 789, 885, 1070, 1250, 1434
SatI GCNGC 8 cut(s) 28, 187, 219, 227, 748, 1056, 1341, 1350
Sau3AI GATC 4 cut(s) 454, 871, 898, 1195
Sau96I GGNCC 5 cut(s) 112, 126, 348, 349, 1167
ScaI AGTACT 1 cut(s) 1272
SchI GAGTC 2 cut(s) 219, 276
ScrFI CCNGG 4 cut(s) 130, 193, 452, 1181
SduI GDGCHC 3 cut(s) 198, 352, 1430
SfaNI GCATC 4 cut(s) 155, 303, 1352, 1404
SfcI CTRYAG 4 cut(s) 745, 1053, 1274, 1347
SfiI GGCCNNNNNGGCC 1 cut(s) 46
SfoI GGCGCC 2 cut(s) 31, 433
Sfr274I CTCGAG 1 cut(s) 422
Sfr303I CCGCGG 1 cut(s) 230
SgrBI CCGCGG 1 cut(s) 230
SinI GGWCC 1 cut(s) 1167
SlaI CTCGAG 1 cut(s) 422
SmiMI CAYNNNNRTG 4 cut(s) 74, 546, 941, 1127
SmlI CTYRAG 2 cut(s) 422, 638
SmoI CTYRAG 2 cut(s) 422, 638
Sse9I AATT 5 cut(s) 438, 588, 712, 1048, 1364
SspDI GGCGCC 2 cut(s) 29, 431
SspMI CTAG 1 cut(s) 1337
StyD4I CCNGG 4 cut(s) 128, 191, 450, 1179
TaaI ACNGT 4 cut(s) 241, 596, 730, 851
TaiI ACGT 4 cut(s) 47, 287, 756, 1392
TaqI TCGA 5 cut(s) 248, 287, 423, 1087, 1284
TaqII GACCGA 1 cut(s) 124
TasI AATT 5 cut(s) 438, 588, 712, 1048, 1364
TatI WGTACW 2 cut(s) 827, 1270
TauI GCSGC 3 cut(s) 30, 221, 229
TfiI GAWTC 3 cut(s) 5, 821, 909
Tru1I TTAA 6 cut(s) 393, 789, 885, 1070, 1250, 1434
Tru9I TTAA 6 cut(s) 393, 789, 885, 1070, 1250, 1434
TscAI CASTG 1 cut(s) 808
TseFI GTSAC 1 cut(s) 653
TseI GCWGC 5 cut(s) 186, 747, 1055, 1340, 1349
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 6 cut(s) 530, 686, 722, 813, 1219, 1314
TspGWI ACGGA 3 cut(s) 291, 308, 572
TspRI CASTG 1 cut(s) 808
VpaK11BI GGWCC 1 cut(s) 1167
XapI RAATTY 2 cut(s) 1048, 1364
XceI RCATGY 2 cut(s) 325, 1043
XhoI CTCGAG 1 cut(s) 422
XmiI GTMKAC 1 cut(s) 644
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 1 cut(s) 1337
ZraI GACGTC 1 cut(s) 285
ZrmI AGTACT 1 cut(s) 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.