RchiOBHm_Chr2g0120901

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0016048
Physical Location & Seq
Reverse (-)
33714281 .. 33718269
3989 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49348

Sequence Viewer

Length: 1377 bp
ATGGATTCCGAGAAATCCATGTTCGGCGGCGCCATCAGGCCGACGTGGCCGTCTCTGCTTCTCCTCTTGACCACCATGCTCGTCCTCTCCTACCAAATCTCCACCATGACGGGCCTCCCCAGCTGGGCCGGGTCGGTCTGGGGAGCATCCGAAACCGCTCCGGCCGACCCTACGAGGAGCAGGAGCTGCTCCGGGTTCTTCTTCGGCGGAGTAGGCGCGGCACCGCCGCGGAGGAAGACGGTGATGTCGATAAGAGACTTCGGCGGAGTCGCCGACGGAACGACGTCGAATACGGAAGCGTTTCGGAAGGCGATGCGGTACATGCAGACGAAGCAGGAGAGTGGTGGGGCCCAGTTGAATGTTCCCGAAGGGCGATGGTTGACCGGCAGTTTTAACCTCACCAGTAACTTCACGCTTTTTCTCGAGGAGGGCGCCGTAATTCTCGGCTCCCAGGATCCAAAGGAATGGCCTATTATTGAGCCATTGCCTTCTTATGGAAGAGGGAGAGAGAGGTTAGGAGGAAGGCATATAAGCCTTATTCATGGGGATGGTCTCACAGATGTTGTCATTACAGGTCATAATGGAACAATTGACGGTCAAGGGAAAATGTGGTGGGAACTATGGTGGAACAGAACGCTTGAGTATACGAGAGTTCATCCTGTTTACTGCAGCAACGTCGTTATAAAGGACATGACAATCTTGGCTCCCCTTAATGCTCCTAACACTGACGGCATAGACGCAGATTCATGTACAAATGTGTGCATTGAAGACTGTTACATTGAGAGTGGCGACGATCTTATAGCAGTTAAAAGTGGGTGGGATCATTATGGAATCAAGATGGCTCGTCCAAGCTCAAACATCATAGTGAGGAGAGTTTCTGGCACCACTCCCACCTGTTCTGGAGTTGGAATAGGTAGTGAGATGTCTGGTGGGATTTCAAATGTTACCATTGAGGATTTGCATGTTTGGAATTCTGCAGCTGGGGTGCGTTTAAAATCTGATAAGGGTCGAGGGGGTTACATAGAAAATGTTACTATAAGTAACATAAAGATGGAAAGGGTCAAGACACCCATAAAGTTTAGTAGAGGGTCCAATGACCACCCGGACAAAGGATGGGATCCCAAAGCTGTTCCCAAAGTAAAGGGTATTTTCATTAGCAACATTGTCAGCTTAAACTCAACAAAAGCTCCAGTACTGCAGGGTGTCGAGGGTGCTTCCTATGAAGGGATATGCCTCAGGAATGTAACGATACTTGGTCTAGCAGCATCTGCAGCGTGGCAATGTGAATTTGTTGCCGGTTTTGCTAATGACGTATTTCCAACGCCATGTCCTCAGATGCAGGATATGGGCTCTTTTAACTGGTGTCAGCAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

458

Amino Acids

49.69

Weight (kDa)

6.47

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 82 - 329 2.4e-07 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 222 - 431 1.9e-29 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33440
fragaria_vesca FvH4_1g24350
malus_domestica MD15G1441700.v1.1
prunus_persica Prupe.4G271300_v2.0.a1
pyrus_communis pycom15g38970
rosa_chinensis RchiOBHm_Chr2g0120901
rosa_laevigata RLG00000018600
rosa_multiflora Rmu_sc0000621.1_g000016
rosa_roxburghii Rroxscaffold_2G00122750
rosa_rugosa Rorug02G0232600
rosa_samantha Rh2AG289000 Rh2BG300000 Rh2CG278000 Rh2DG314000
rosa_wichuraiana Rw2G023150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 683
AasI GACNNNNNNGTC 2 cut(s) 49, 244
AatII GACGTC 1 cut(s) 287
AccB1I GGYRCC 4 cut(s) 29, 220, 431, 881
AccBSI CCGCTC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 644
AccII CGCG 2 cut(s) 218, 229
AciI CCGC 9 cut(s) 27, 156, 207, 218, 224, 227, 229, 264, 316
AclWI GGATC 5 cut(s) 449, 462, 828, 1112, 1125
AcoI YGGCCR 2 cut(s) 47, 162
AcsI RAATTY 2 cut(s) 970, 1286
AcyI GRCGYC 3 cut(s) 30, 284, 432
AfaI GTAC 3 cut(s) 320, 751, 1194
AfiI CCNNNNNNNGG 4 cut(s) 124, 494, 1109, 1224
AgsI TTSAA 3 cut(s) 358, 767, 939
AjiI CACGTC 1 cut(s) 45
AjnI CCWGG 1 cut(s) 450
AluBI AGCT 7 cut(s) 123, 186, 852, 980, 1127, 1170, 1187
AluI AGCT 7 cut(s) 123, 186, 852, 980, 1127, 1170, 1187
Alw26I GTCTC 3 cut(s) 57, 249, 557
AlwI GGATC 5 cut(s) 449, 462, 828, 1112, 1125
AlwNI CAGNNNCTG 2 cut(s) 186, 1268
Ama87I CYCGRG 1 cut(s) 422
AoxI GGCC 7 cut(s) 38, 47, 112, 126, 162, 348, 467
ApaI GGGCCC 1 cut(s) 352
ApeKI GCWGC 5 cut(s) 186, 669, 977, 1262, 1271
ApoI RAATTY 2 cut(s) 970, 1286
Asp700I GAANNNNTTC 1 cut(s) 300
AspLEI GCGC 3 cut(s) 32, 218, 434
AspS9I GGNCC 5 cut(s) 112, 126, 348, 349, 1089
AsuC2I CCSGG 3 cut(s) 130, 193, 1103
AsuHPI GGTGA 2 cut(s) 253, 391
AvaI CYCGRG 1 cut(s) 422
AvaII GGWCC 1 cut(s) 1089
AxyI CCTNAGG 1 cut(s) 1235
BaeGI GKGCMC 1 cut(s) 352
BamHI GGATCC 2 cut(s) 454, 1117
BanI GGYRCC 4 cut(s) 29, 220, 431, 881
BanII GRGCYC 2 cut(s) 352, 1352
BbsI GAAGAC 2 cut(s) 242, 774
BbvI GCAGC 5 cut(s) 173, 681, 989, 1274, 1283
BccI CCATC 6 cut(s) 41, 369, 542, 832, 1045, 1107
BceAI ACGGC 3 cut(s) 34, 419, 745
BcgI CGANNNNNNTGC 2 cut(s) 658, 692
BciT130I CCWGG 1 cut(s) 452
BcnI CCSGG 3 cut(s) 130, 193, 1103
BcoDI GTCTC 3 cut(s) 57, 249, 557
BfaI CTAG 1 cut(s) 1259
BfmI CTRYAG 4 cut(s) 667, 975, 1196, 1269
BfoI RGCGCY 2 cut(s) 33, 435
BglI GCCNNNNNGGC 1 cut(s) 46
BisI GCNGC 8 cut(s) 28, 187, 219, 227, 670, 978, 1263, 1272
BlsI GCNGC 8 cut(s) 29, 188, 220, 228, 671, 979, 1264, 1273
BmcAI AGTACT 1 cut(s) 1194
Bme1390I CCNGG 4 cut(s) 130, 193, 452, 1103
Bme18I GGWCC 1 cut(s) 1089
BmeT110I CYCGRG 1 cut(s) 422
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 5 cut(s) 112, 126, 348, 349, 1089
BmrFI CCNGG 4 cut(s) 130, 193, 452, 1103
BmrI ACTGGG 1 cut(s) 346
BmsI GCATC 4 cut(s) 155, 303, 1274, 1326
BmuI ACTGGG 1 cut(s) 346
BpiI GAAGAC 2 cut(s) 242, 774
BpmI CTGGAG 2 cut(s) 921, 1173
BpuEI CTTGAG 1 cut(s) 659
BpuMI CCSGG 3 cut(s) 130, 193, 1103
BsaHI GRCGYC 3 cut(s) 30, 284, 432
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 2 cut(s) 227, 450
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 1171, 1201
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 494, 1109, 1224
Bse118I RCCGGY 2 cut(s) 383, 1295
Bse1I ACTGG 4 cut(s) 352, 402, 1190, 1364
Bse21I CCTNAGG 1 cut(s) 1235
Bse3DI GCAATG 2 cut(s) 482, 1286
BseBI CCWGG 1 cut(s) 452
BseDI CCNNGG 2 cut(s) 227, 450
BseGI GGATG 4 cut(s) 146, 553, 655, 1118
BseLI CCNNNNNNNGG 4 cut(s) 124, 494, 1109, 1224
BseMI GCAATG 2 cut(s) 482, 1286
BseMII CTCAG 2 cut(s) 1249, 1346
BseNI ACTGG 4 cut(s) 352, 402, 1190, 1364
BseRI GAGGAG 4 cut(s) 53, 190, 440, 883
BseSI GKGCMC 1 cut(s) 352
BseX3I CGGCCG 1 cut(s) 162
BseXI GCAGC 5 cut(s) 173, 681, 989, 1274, 1283
BseYI CCCAGC 3 cut(s) 119, 123, 980
Bsh1236I CGCG 2 cut(s) 218, 229
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
BshNI GGYRCC 4 cut(s) 29, 220, 431, 881
BsiEI CGRYCG 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 422
BsiSI CCGG 6 cut(s) 129, 161, 192, 384, 1103, 1296
BslI CCNNNNNNNGG 4 cut(s) 124, 494, 1109, 1224
BsmAI GTCTC 3 cut(s) 57, 249, 557
BsmBI CGTCTC 1 cut(s) 57
BsnI GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
Bso31I GGTCTC 1 cut(s) 557
BsoBI CYCGRG 1 cut(s) 422
Bsp120I GGGCCC 1 cut(s) 348
Bsp1286I GDGCHC 2 cut(s) 352, 1352
Bsp1407I TGTACA 1 cut(s) 749
Bsp143I GATC 4 cut(s) 454, 793, 820, 1117
BspACI CCGC 9 cut(s) 27, 156, 207, 218, 224, 227, 229, 264, 316
BspANI GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
BspCNI CTCAG 2 cut(s) 1248, 1345
BspFNI CGCG 2 cut(s) 218, 229
BspMAI CTGCAG 4 cut(s) 671, 979, 1200, 1273
BspPI GGATC 5 cut(s) 449, 462, 828, 1112, 1125
BspT107I GGYRCC 4 cut(s) 29, 220, 431, 881
BspTNI GGTCTC 1 cut(s) 557
BsrBI CCGCTC 1 cut(s) 158
BsrDI GCAATG 2 cut(s) 482, 1286
BsrFI RCCGGY 2 cut(s) 383, 1295
BsrGI TGTACA 1 cut(s) 749
BsrI ACTGG 4 cut(s) 352, 402, 1190, 1364
BssAI RCCGGY 2 cut(s) 383, 1295
BssECI CCNNGG 2 cut(s) 227, 450
BssMI GATC 4 cut(s) 454, 793, 820, 1117
BssNAI GTATAC 1 cut(s) 645
BssNI GRCGYC 3 cut(s) 30, 284, 432
Bst1107I GTATAC 1 cut(s) 645
Bst2UI CCWGG 1 cut(s) 452
Bst4CI ACNGT 3 cut(s) 241, 596, 773
Bst6I CTCTTC 1 cut(s) 493
BstACI GRCGYC 3 cut(s) 30, 284, 432
BstAPI GCANNNNNTGC 2 cut(s) 186, 1268
BstAUI TGTACA 1 cut(s) 749
BstDEI CTNAG 2 cut(s) 1235, 1332
BstDSI CCRYGG 1 cut(s) 227
BstF5I GGATG 4 cut(s) 146, 553, 655, 1118
BstFNI CGCG 2 cut(s) 218, 229
BstH2I RGCGCY 2 cut(s) 33, 435
BstHHI GCGC 3 cut(s) 32, 218, 434
BstKTI GATC 4 cut(s) 457, 796, 823, 1120
BstMAI GTCTC 3 cut(s) 57, 249, 557
BstMBI GATC 4 cut(s) 454, 793, 820, 1117
BstMCI CGRYCG 1 cut(s) 165
BstNI CCWGG 1 cut(s) 452
BstNSI RCATGY 2 cut(s) 325, 965
BstSCI CCNGG 4 cut(s) 128, 191, 450, 1101
BstSFI CTRYAG 4 cut(s) 667, 975, 1196, 1269
BstSLI GKGCMC 1 cut(s) 352
BstUI CGCG 2 cut(s) 218, 229
BstV1I GCAGC 5 cut(s) 173, 681, 989, 1274, 1283
BstV2I GAAGAC 2 cut(s) 242, 774
BstX2I RGATCY 2 cut(s) 454, 1117
BstXI CCANNNNNNTGG 1 cut(s) 465
BstYI RGATCY 2 cut(s) 454, 1117
BstZ17I GTATAC 1 cut(s) 645
BstZI CGGCCG 1 cut(s) 162
Bsu36I CCTNAGG 1 cut(s) 1235
BsuRI GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
BtgI CCRYGG 1 cut(s) 227
BtgZI GCGATG 2 cut(s) 326, 388
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 4 cut(s) 146, 553, 655, 1118
BtsIMutI CAGTG 1 cut(s) 723
CaiI CAGNNNCTG 2 cut(s) 186, 1268
CfoI GCGC 3 cut(s) 32, 218, 434
Cfr10I RCCGGY 2 cut(s) 383, 1295
Cfr13I GGNCC 5 cut(s) 112, 126, 348, 349, 1089
Cfr42I CCGCGG 1 cut(s) 230
CseI GACGC 1 cut(s) 746
Csp6I GTAC 3 cut(s) 319, 750, 1193
CviAII CATG 9 cut(s) 19, 76, 106, 322, 542, 691, 747, 962, 1326
CviQI GTAC 3 cut(s) 319, 750, 1193
DdeI CTNAG 2 cut(s) 1235, 1332
DinI GGCGCC 2 cut(s) 31, 433
DpnI GATC 4 cut(s) 456, 795, 822, 1119
DpnII GATC 4 cut(s) 454, 793, 820, 1117
DraI TTTAAA 1 cut(s) 993
DrdI GACNNNNNNGTC 2 cut(s) 49, 244
DseDI GACNNNNNNGTC 2 cut(s) 49, 244
EaeI YGGCCR 2 cut(s) 47, 162
EagI CGGCCG 1 cut(s) 162
Eam1104I CTCTTC 1 cut(s) 493
EarI CTCTTC 1 cut(s) 493
EciI GGCGGA 2 cut(s) 222, 279
EclXI CGGCCG 1 cut(s) 162
Eco24I GRGCYC 2 cut(s) 352, 1352
Eco31I GGTCTC 1 cut(s) 557
Eco47I GGWCC 1 cut(s) 1089
Eco52I CGGCCG 1 cut(s) 162
Eco81I CCTNAGG 1 cut(s) 1235
Eco88I CYCGRG 1 cut(s) 422
EcoO109I RGGNCCY 1 cut(s) 348
EcoRI GAATTC 1 cut(s) 970
EcoRII CCWGG 1 cut(s) 450
EcoT38I GRGCYC 2 cut(s) 352, 1352
EgeI GGCGCC 2 cut(s) 31, 433
EheI GGCGCC 2 cut(s) 31, 433
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 9 cut(s) 22, 79, 109, 325, 545, 694, 750, 965, 1329
FatI CATG 9 cut(s) 18, 75, 105, 321, 541, 690, 746, 961, 1325
FblI GTMKAC 1 cut(s) 644
Fnu4HI GCNGC 8 cut(s) 28, 187, 219, 227, 670, 978, 1263, 1272
FokI GGATG 4 cut(s) 133, 560, 642, 1125
FriOI GRGCYC 2 cut(s) 352, 1352
Fsp4HI GCNGC 8 cut(s) 28, 187, 219, 227, 670, 978, 1263, 1272
FspBI CTAG 1 cut(s) 1259
GlaI GCGC 3 cut(s) 31, 217, 433
GluI GCNGC 8 cut(s) 28, 187, 219, 227, 670, 978, 1263, 1272
GsaI CCCAGC 3 cut(s) 123, 127, 984
GsuI CTGGAG 2 cut(s) 921, 1173
HaeII RGCGCY 2 cut(s) 33, 435
HaeIII GGCC 7 cut(s) 40, 49, 114, 128, 164, 350, 469
HapII CCGG 6 cut(s) 129, 161, 192, 384, 1103, 1296
HgaI GACGC 1 cut(s) 746
HhaI GCGC 3 cut(s) 32, 218, 434
Hin1I GRCGYC 3 cut(s) 30, 284, 432
Hin1II CATG 9 cut(s) 22, 79, 109, 325, 545, 694, 750, 965, 1329
Hin6I GCGC 3 cut(s) 30, 216, 432
HinP1I GCGC 3 cut(s) 30, 216, 432
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HinfI GANTC 4 cut(s) 5, 267, 743, 831
HpaII CCGG 6 cut(s) 129, 161, 192, 384, 1103, 1296
HphI GGTGA 2 cut(s) 253, 391
Hpy166II GTNNAC 3 cut(s) 381, 645, 664
Hpy188I TCNGA 5 cut(s) 10, 151, 306, 1000, 1335
Hpy188III TCNNGA 7 cut(s) 67, 365, 422, 835, 900, 1063, 1237
Hpy8I GTNNAC 3 cut(s) 381, 645, 664
Hpy99I CGWCG 6 cut(s) 46, 278, 286, 289, 680, 794
HpyAV CCTTC 5 cut(s) 301, 362, 498, 516, 1217
HpyCH4III ACNGT 3 cut(s) 241, 596, 773
HpyCH4IV ACGT 4 cut(s) 44, 284, 675, 1311
HpyCH4V TGCA 8 cut(s) 325, 669, 762, 961, 977, 1198, 1271, 1339
HpyF3I CTNAG 2 cut(s) 1235, 1332
HpySE526I ACGT 4 cut(s) 44, 284, 675, 1311
Hsp92I GRCGYC 3 cut(s) 30, 284, 432
Hsp92II CATG 9 cut(s) 22, 79, 109, 325, 545, 694, 750, 965, 1329
HspAI GCGC 3 cut(s) 30, 216, 432
KasI GGCGCC 2 cut(s) 29, 431
KspI CCGCGG 1 cut(s) 230
Kzo9I GATC 4 cut(s) 454, 793, 820, 1117
LmnI GCTCC 9 cut(s) 143, 163, 177, 183, 194, 452, 709, 721, 1192
Lsp1109I GCAGC 5 cut(s) 173, 681, 989, 1274, 1283
LweI GCATC 4 cut(s) 155, 303, 1274, 1326
MaeI CTAG 1 cut(s) 1259
MaeII ACGT 4 cut(s) 44, 284, 675, 1311
MaeIII GTNAC 7 cut(s) 404, 773, 943, 1016, 1030, 1040, 1243
MalI GATC 4 cut(s) 456, 795, 822, 1119
MbiI CCGCTC 1 cut(s) 158
MboI GATC 4 cut(s) 454, 793, 820, 1117
MboII GAAGA 5 cut(s) 190, 193, 247, 510, 779
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 2 cut(s) 454, 1117
MhlI GDGCHC 2 cut(s) 352, 1352
MluCI AATT 4 cut(s) 438, 588, 970, 1286
Mly113I GGCGCC 2 cut(s) 30, 432
MlyI GAGTC 1 cut(s) 276
MmeI TCCRAC 2 cut(s) 886, 1343
MroXI GAANNNNTTC 1 cut(s) 300
MseI TTAA 6 cut(s) 393, 711, 807, 992, 1172, 1356
MslI CAYNNNNRTG 3 cut(s) 546, 863, 1049
MspA1I CMGCKG 3 cut(s) 123, 229, 980
MspI CCGG 6 cut(s) 129, 161, 192, 384, 1103, 1296
MspR9I CCNGG 4 cut(s) 130, 193, 452, 1103
MunI CAATTG 1 cut(s) 588
MvaI CCWGG 1 cut(s) 452
MvnI CGCG 2 cut(s) 218, 229
NarI GGCGCC 2 cut(s) 30, 432
NciI CCSGG 3 cut(s) 130, 193, 1103
NdeII GATC 4 cut(s) 454, 793, 820, 1117
NlaIII CATG 9 cut(s) 22, 79, 109, 325, 545, 694, 750, 965, 1329
NmeAIII GCCGAG 1 cut(s) 423
NspI RCATGY 2 cut(s) 325, 965
PaeR7I CTCGAG 1 cut(s) 422
PcsI WCGNNNNNNNCGW 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 3 cut(s) 5, 743, 831
PkrI GCNGC 8 cut(s) 29, 188, 220, 228, 671, 979, 1264, 1273
PleI GAGTC 1 cut(s) 275
PluTI GGCGCC 2 cut(s) 33, 435
PpsI GAGTC 1 cut(s) 275
PsiI TTATAA 1 cut(s) 683
Psp6I CCWGG 1 cut(s) 450
PspFI CCCAGC 3 cut(s) 119, 123, 980
PspGI CCWGG 1 cut(s) 450
PspOMI GGGCCC 1 cut(s) 348
PspPI GGNCC 5 cut(s) 112, 126, 348, 349, 1089
PstI CTGCAG 4 cut(s) 671, 979, 1200, 1273
PstNI CAGNNNCTG 2 cut(s) 186, 1268
PsuI RGATCY 2 cut(s) 454, 1117
PvuII CAGCTG 2 cut(s) 123, 980
RsaI GTAC 3 cut(s) 320, 751, 1194
RsaNI GTAC 3 cut(s) 319, 750, 1193
RseI CAYNNNNRTG 3 cut(s) 546, 863, 1049
SacII CCGCGG 1 cut(s) 230
SaqAI TTAA 6 cut(s) 393, 711, 807, 992, 1172, 1356
SatI GCNGC 8 cut(s) 28, 187, 219, 227, 670, 978, 1263, 1272
Sau3AI GATC 4 cut(s) 454, 793, 820, 1117
Sau96I GGNCC 5 cut(s) 112, 126, 348, 349, 1089
ScaI AGTACT 1 cut(s) 1194
SchI GAGTC 1 cut(s) 276
ScrFI CCNGG 4 cut(s) 130, 193, 452, 1103
SduI GDGCHC 2 cut(s) 352, 1352
SfaNI GCATC 4 cut(s) 155, 303, 1274, 1326
SfcI CTRYAG 4 cut(s) 667, 975, 1196, 1269
SfiI GGCCNNNNNGGCC 1 cut(s) 46
SfoI GGCGCC 2 cut(s) 31, 433
Sfr274I CTCGAG 1 cut(s) 422
Sfr303I CCGCGG 1 cut(s) 230
SgrBI CCGCGG 1 cut(s) 230
SinI GGWCC 1 cut(s) 1089
SlaI CTCGAG 1 cut(s) 422
SmiMI CAYNNNNRTG 3 cut(s) 546, 863, 1049
SmlI CTYRAG 2 cut(s) 422, 638
SmoI CTYRAG 2 cut(s) 422, 638
Sse9I AATT 4 cut(s) 438, 588, 970, 1286
SsiI CCGC 9 cut(s) 27, 156, 207, 218, 224, 227, 229, 264, 316
SspDI GGCGCC 2 cut(s) 29, 431
SspMI CTAG 1 cut(s) 1259
StyD4I CCNGG 4 cut(s) 128, 191, 450, 1101
TaaI ACNGT 3 cut(s) 241, 596, 773
TaiI ACGT 4 cut(s) 47, 287, 678, 1314
TaqI TCGA 5 cut(s) 248, 287, 423, 1009, 1206
TaqII GACCGA 1 cut(s) 124
TasI AATT 4 cut(s) 438, 588, 970, 1286
TatI WGTACW 2 cut(s) 749, 1192
TauI GCSGC 3 cut(s) 30, 221, 229
TfiI GAWTC 3 cut(s) 5, 743, 831
Tru1I TTAA 6 cut(s) 393, 711, 807, 992, 1172, 1356
Tru9I TTAA 6 cut(s) 393, 711, 807, 992, 1172, 1356
TscAI CASTG 1 cut(s) 730
TseI GCWGC 5 cut(s) 186, 669, 977, 1262, 1271
TspDTI ATGAA 5 cut(s) 530, 644, 735, 1141, 1236
TspGWI ACGGA 2 cut(s) 291, 308
TspRI CASTG 1 cut(s) 730
VpaK11BI GGWCC 1 cut(s) 1089
XapI RAATTY 2 cut(s) 970, 1286
XceI RCATGY 2 cut(s) 325, 965
XhoI CTCGAG 1 cut(s) 422
XmiI GTMKAC 1 cut(s) 644
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 1 cut(s) 1259
ZraI GACGTC 1 cut(s) 285
ZrmI AGTACT 1 cut(s) 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.