Rroxscaffold_2G00122750

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016048
Physical Location & Seq
Forward (+)
56162425 .. 56166054
3630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00122750.1

Sequence Viewer

Length: 1452 bp
ATGGATTCCGAGAAGTCCATATTCAACGGCGCCATCAGGCCGACGTGGCCGTCTCTGCTCCTCCTTTTCACCACCATGCTCGTCCTCTCCTACCAAATCTCCACCATGACGGGCCTCCCGAGCTGGGCCGGGTCGGTCTGGGGAGGATCCGAAACCGCTCCGGCCGACCCGACGAGGAGCAGGACCTGCTCCGGGTTCTTCATCGGCGGAGTCGGCGCGGCACCGCGGAGGAAGACGGTGATGTCGATAAGAGACTTCGGTGGAGTCGGCGACGGGACGACGTCGAATACGGAAGCGTTTCGGAAGGCGACACGGTACATGCAGACGAAGCGGGAGAGTGGCGGGGCCCAGTTGAATGTTCCCGAAGGGCGATGGTTGACCGGCAGTTTTAACCTCACCAGTAACTTCACGCTTTTTCTCGAGGAGGGCGCTGTAATTCTCGGCTCCCAGGATCCAAAGGAATGGCCTATTATTGAGCCATTGCCTTCTTATGGAAGAGGGAGAGAGAGGGTAGGAGGAAGGCATATAAGCCTTATTCATGGGGATGGTCTCACAGATGTTGTCATTACAGGTCATAATGGAACAATTGACGGTCAAGGGAAAATGTGGTGGGAACTATGGTGGAACAGAACGCTCAAGTATACGAGAGGTCACCTTCTTGAACTAATGAACTCTAACAACATTCTCATCTCTAACCTCACCTTGCTCAATTCTCCTTTTTGGACAGTTCATCCTGTTTACTGCAGCAATGTTGTTATAAAGGACATGACAATCTTGGCTCCCCTTAATGCTCCTAACACTGACGGCATAGACGCAGATTCATGTACAAATGTGTGCATTGAAGACTGTTACATTGAGAGTGGCGACGATCTTATAGCAGTTAAAAGTGGGTGGGATCATTATGGAATCAAGATGGCTCATCCAAGCTCAAACATCATAGTGAGGAGAGTTTCTGGTACCACTCCCACCTGTTCTGGAGTTGGAATAGGTAGTGAGATGTCTGGTGGGATTTCAAATGTTACCATTGAGGATTTGCATGTTTGGAATTCTGCAGCCGGGGTGCGTTTAAAATCTGATAAGGGTCGAGGGGGTTACATAGAAAATGTTACTATAAGTAACATAAAGATGGAAAGGGTCAAGACACCCATAAAGTTTAGTAGAGGGTCCAATGACCACCCGGACAAAGGATGGGATCCCAAAGCTGTTCCCAAAGTAAAGGGTATTTTCATTAGCAACATTGTCAGCTTAAACTCAACAAAAGCTCCAGTACTGCAGGGTGTCGAGGGTGCTTCCTATGAAGGGATATGCCTCAGGAATGTAACGATACTTGGTCTAGCAGCATCTGCAGCGTGGCAATGTGAATTTGTTTCCGGTTTTGCTAATGACGTATTTCCAACGCCATGTCCTCAGATGCAGGATATGGGCTGTTTTGACTGGTGTCAGCAACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

483

Amino Acids

52.58

Weight (kDa)

6.76

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 178 - 456 8.5e-43 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33440
fragaria_vesca FvH4_1g24350
malus_domestica MD15G1441700.v1.1
prunus_persica Prupe.4G271300_v2.0.a1
pyrus_communis pycom15g38970
rosa_chinensis RchiOBHm_Chr2g0120901
rosa_laevigata RLG00000018600
rosa_multiflora Rmu_sc0000621.1_g000016
rosa_roxburghii Rroxscaffold_2G00122750
rosa_rugosa Rorug02G0232600
rosa_samantha Rh2AG289000 Rh2BG300000 Rh2CG278000 Rh2DG314000
rosa_wichuraiana Rw2G023150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 758
AasI GACNNNNNNGTC 2 cut(s) 49, 241
AatII GACGTC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 194
Acc65I GGTACC 1 cut(s) 956
AccB1I GGYRCC 3 cut(s) 29, 220, 956
AccBSI CCGCTC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 641
AccII CGCG 2 cut(s) 218, 226
AciI CCGC 7 cut(s) 156, 207, 218, 224, 226, 331, 342
AclI AACGTT 1 cut(s) 1447
AclWI GGATC 7 cut(s) 141, 154, 446, 459, 903, 1187, 1200
AcoI YGGCCR 2 cut(s) 47, 162
AcsI RAATTY 2 cut(s) 1045, 1361
AcyI GRCGYC 2 cut(s) 30, 281
AfaI GTAC 4 cut(s) 317, 826, 958, 1269
AfiI CCNNNNNNNGG 5 cut(s) 124, 192, 491, 1184, 1299
AgsI TTSAA 5 cut(s) 25, 355, 662, 842, 1014
AjiI CACGTC 1 cut(s) 45
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 5 cut(s) 123, 927, 1202, 1245, 1262
AluI AGCT 5 cut(s) 123, 927, 1202, 1245, 1262
Alw26I GTCTC 3 cut(s) 57, 246, 554
AlwI GGATC 7 cut(s) 141, 154, 446, 459, 903, 1187, 1200
AlwNI CAGNNNCTG 2 cut(s) 186, 1343
Ama87I CYCGRG 2 cut(s) 118, 419
AoxI GGCC 7 cut(s) 38, 47, 112, 126, 162, 345, 464
ApaI GGGCCC 1 cut(s) 349
ApeKI GCWGC 4 cut(s) 744, 1052, 1337, 1346
ApoI RAATTY 2 cut(s) 1045, 1361
Asp700I GAANNNNTTC 1 cut(s) 297
Asp718I GGTACC 1 cut(s) 956
AspLEI GCGC 3 cut(s) 32, 218, 431
AspS9I GGNCC 6 cut(s) 112, 126, 183, 345, 346, 1164
AsuC2I CCSGG 4 cut(s) 130, 193, 1057, 1178
AsuHPI GGTGA 5 cut(s) 61, 250, 388, 644, 691
AvaI CYCGRG 2 cut(s) 118, 419
AvaII GGWCC 2 cut(s) 183, 1164
AxyI CCTNAGG 1 cut(s) 1310
BaeGI GKGCMC 1 cut(s) 349
BamHI GGATCC 3 cut(s) 146, 451, 1192
BanI GGYRCC 3 cut(s) 29, 220, 956
BanII GRGCYC 1 cut(s) 349
BbsI GAAGAC 2 cut(s) 239, 849
BbvI GCAGC 4 cut(s) 756, 1064, 1349, 1358
BccI CCATC 6 cut(s) 41, 366, 539, 907, 1120, 1182
BceAI ACGGC 3 cut(s) 34, 43, 820
BciT130I CCWGG 1 cut(s) 449
BcnI CCSGG 4 cut(s) 130, 193, 1057, 1178
BcoDI GTCTC 3 cut(s) 57, 246, 554
BfaI CTAG 1 cut(s) 1334
BfmI CTRYAG 4 cut(s) 742, 1050, 1271, 1344
BfoI RGCGCY 2 cut(s) 33, 432
BfuAI ACCTGC 1 cut(s) 194
BglI GCCNNNNNGGC 1 cut(s) 46
BisI GCNGC 5 cut(s) 219, 745, 1053, 1338, 1347
BlsI GCNGC 5 cut(s) 220, 746, 1054, 1339, 1348
BmcAI AGTACT 1 cut(s) 1269
Bme1390I CCNGG 5 cut(s) 130, 193, 449, 1057, 1178
Bme18I GGWCC 2 cut(s) 183, 1164
BmeT110I CYCGRG 2 cut(s) 118, 419
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 6 cut(s) 112, 126, 183, 345, 346, 1164
BmrFI CCNGG 5 cut(s) 130, 193, 449, 1057, 1178
BmrI ACTGGG 1 cut(s) 343
BmsI GCATC 2 cut(s) 1349, 1401
BmuI ACTGGG 1 cut(s) 343
BoxI GACNNNNGTC 1 cut(s) 1437
BpiI GAAGAC 2 cut(s) 239, 849
BpmI CTGGAG 2 cut(s) 996, 1248
BpuEI CTTGAG 1 cut(s) 620
BpuMI CCSGG 4 cut(s) 130, 193, 1057, 1178
BsaHI GRCGYC 2 cut(s) 30, 281
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 3 cut(s) 224, 447, 1056
BsaWI WCCGGW 1 cut(s) 1370
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 1246, 1276
Bsc4I CCNNNNNNNGG 5 cut(s) 124, 192, 491, 1184, 1299
Bse118I RCCGGY 1 cut(s) 380
Bse1I ACTGG 4 cut(s) 349, 399, 1265, 1439
Bse21I CCTNAGG 1 cut(s) 1310
Bse3DI GCAATG 3 cut(s) 479, 754, 1361
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 3 cut(s) 224, 447, 1056
BseGI GGATG 4 cut(s) 550, 730, 919, 1193
BseLI CCNNNNNNNGG 5 cut(s) 124, 192, 491, 1184, 1299
BseMI GCAATG 3 cut(s) 479, 754, 1361
BseMII CTCAG 2 cut(s) 1324, 1421
BseNI ACTGG 4 cut(s) 349, 399, 1265, 1439
BseRI GAGGAG 4 cut(s) 50, 190, 437, 958
BseSI GKGCMC 1 cut(s) 349
BseX3I CGGCCG 1 cut(s) 162
BseXI GCAGC 4 cut(s) 756, 1064, 1349, 1358
BseYI CCCAGC 1 cut(s) 123
Bsh1236I CGCG 2 cut(s) 218, 226
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 7 cut(s) 40, 49, 114, 128, 164, 347, 466
BshNI GGYRCC 3 cut(s) 29, 220, 956
BsiEI CGRYCG 1 cut(s) 165
BsiHKCI CYCGRG 2 cut(s) 118, 419
BsiSI CCGG 7 cut(s) 129, 161, 192, 381, 1056, 1178, 1371
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 5 cut(s) 124, 192, 491, 1184, 1299
BsmAI GTCTC 3 cut(s) 57, 246, 554
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 289
BsnI GGCC 7 cut(s) 40, 49, 114, 128, 164, 347, 466
Bso31I GGTCTC 1 cut(s) 554
BsoBI CYCGRG 2 cut(s) 118, 419
Bsp120I GGGCCC 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 349
Bsp1407I TGTACA 1 cut(s) 824
Bsp143I GATC 5 cut(s) 146, 451, 868, 895, 1192
BspACI CCGC 7 cut(s) 156, 207, 218, 224, 226, 331, 342
BspANI GGCC 7 cut(s) 40, 49, 114, 128, 164, 347, 466
BspCNI CTCAG 2 cut(s) 1323, 1420
BspFNI CGCG 2 cut(s) 218, 226
BspMAI CTGCAG 4 cut(s) 746, 1054, 1275, 1348
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 7 cut(s) 141, 154, 446, 459, 903, 1187, 1200
BspT107I GGYRCC 3 cut(s) 29, 220, 956
BspTNI GGTCTC 1 cut(s) 554
BsrBI CCGCTC 1 cut(s) 158
BsrDI GCAATG 3 cut(s) 479, 754, 1361
BsrFI RCCGGY 1 cut(s) 380
BsrGI TGTACA 1 cut(s) 824
BsrI ACTGG 4 cut(s) 349, 399, 1265, 1439
BssAI RCCGGY 1 cut(s) 380
BssECI CCNNGG 3 cut(s) 224, 447, 1056
BssMI GATC 5 cut(s) 146, 451, 868, 895, 1192
BssNAI GTATAC 1 cut(s) 642
BssNI GRCGYC 2 cut(s) 30, 281
Bst1107I GTATAC 1 cut(s) 642
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 5 cut(s) 238, 315, 593, 727, 848
Bst6I CTCTTC 1 cut(s) 490
BstACI GRCGYC 2 cut(s) 30, 281
BstAPI GCANNNNNTGC 2 cut(s) 186, 1343
BstAUI TGTACA 1 cut(s) 824
BstDEI CTNAG 2 cut(s) 1310, 1407
BstDSI CCRYGG 1 cut(s) 224
BstEII GGTNACC 1 cut(s) 650
BstF5I GGATG 4 cut(s) 550, 730, 919, 1193
BstFNI CGCG 2 cut(s) 218, 226
BstH2I RGCGCY 2 cut(s) 33, 432
BstHHI GCGC 3 cut(s) 32, 218, 431
BstKTI GATC 5 cut(s) 149, 454, 871, 898, 1195
BstMAI GTCTC 3 cut(s) 57, 246, 554
BstMBI GATC 5 cut(s) 146, 451, 868, 895, 1192
BstMCI CGRYCG 1 cut(s) 165
BstMWI GCNNNNNNNGC 8 cut(s) 46, 55, 120, 186, 213, 328, 1343, 1346
BstNI CCWGG 1 cut(s) 449
BstNSI RCATGY 2 cut(s) 322, 1040
BstPAI GACNNNNGTC 1 cut(s) 1437
BstPI GGTNACC 1 cut(s) 650
BstSCI CCNGG 5 cut(s) 128, 191, 447, 1055, 1176
BstSFI CTRYAG 4 cut(s) 742, 1050, 1271, 1344
BstSLI GKGCMC 1 cut(s) 349
BstUI CGCG 2 cut(s) 218, 226
BstV1I GCAGC 4 cut(s) 756, 1064, 1349, 1358
BstV2I GAAGAC 2 cut(s) 239, 849
BstX2I RGATCY 3 cut(s) 146, 451, 1192
BstXI CCANNNNNNTGG 1 cut(s) 462
BstYI RGATCY 3 cut(s) 146, 451, 1192
BstZ17I GTATAC 1 cut(s) 642
BstZI CGGCCG 1 cut(s) 162
Bsu36I CCTNAGG 1 cut(s) 1310
BsuRI GGCC 7 cut(s) 40, 49, 114, 128, 164, 347, 466
BtgI CCRYGG 1 cut(s) 224
BtgZI GCGATG 1 cut(s) 385
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 4 cut(s) 550, 730, 919, 1193
BtsIMutI CAGTG 1 cut(s) 798
BveI ACCTGC 1 cut(s) 194
CaiI CAGNNNCTG 2 cut(s) 186, 1343
CfoI GCGC 3 cut(s) 32, 218, 431
Cfr10I RCCGGY 1 cut(s) 380
Cfr13I GGNCC 6 cut(s) 112, 126, 183, 345, 346, 1164
Cfr42I CCGCGG 1 cut(s) 227
CseI GACGC 1 cut(s) 821
Csp6I GTAC 4 cut(s) 316, 825, 957, 1268
CviAII CATG 8 cut(s) 76, 106, 319, 539, 766, 822, 1037, 1401
CviQI GTAC 4 cut(s) 316, 825, 957, 1268
DdeI CTNAG 2 cut(s) 1310, 1407
DinI GGCGCC 1 cut(s) 31
DpnI GATC 5 cut(s) 148, 453, 870, 897, 1194
DpnII GATC 5 cut(s) 146, 451, 868, 895, 1192
DraI TTTAAA 1 cut(s) 1068
DrdI GACNNNNNNGTC 2 cut(s) 49, 241
DseDI GACNNNNNNGTC 2 cut(s) 49, 241
EaeI YGGCCR 2 cut(s) 47, 162
EagI CGGCCG 1 cut(s) 162
Eam1104I CTCTTC 1 cut(s) 490
EarI CTCTTC 1 cut(s) 490
EciI GGCGGA 1 cut(s) 222
EclXI CGGCCG 1 cut(s) 162
Eco24I GRGCYC 1 cut(s) 349
Eco31I GGTCTC 1 cut(s) 554
Eco47I GGWCC 2 cut(s) 183, 1164
Eco52I CGGCCG 1 cut(s) 162
Eco81I CCTNAGG 1 cut(s) 1310
Eco88I CYCGRG 2 cut(s) 118, 419
Eco91I GGTNACC 1 cut(s) 650
EcoO109I RGGNCCY 2 cut(s) 183, 345
EcoO65I GGTNACC 1 cut(s) 650
EcoRI GAATTC 1 cut(s) 1045
EcoRII CCWGG 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 349
EgeI GGCGCC 1 cut(s) 31
EheI GGCGCC 1 cut(s) 31
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 8 cut(s) 79, 109, 322, 542, 769, 825, 1040, 1404
FaqI GGGAC 1 cut(s) 289
FatI CATG 8 cut(s) 75, 105, 318, 538, 765, 821, 1036, 1400
FauI CCCGC 2 cut(s) 324, 335
FblI GTMKAC 1 cut(s) 641
Fnu4HI GCNGC 5 cut(s) 219, 745, 1053, 1338, 1347
FokI GGATG 4 cut(s) 557, 717, 906, 1200
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 5 cut(s) 219, 745, 1053, 1338, 1347
FspBI CTAG 1 cut(s) 1334
GlaI GCGC 3 cut(s) 31, 217, 430
GluI GCNGC 5 cut(s) 219, 745, 1053, 1338, 1347
GsaI CCCAGC 1 cut(s) 127
GsuI CTGGAG 2 cut(s) 996, 1248
HaeII RGCGCY 2 cut(s) 33, 432
HaeIII GGCC 7 cut(s) 40, 49, 114, 128, 164, 347, 466
HapII CCGG 7 cut(s) 129, 161, 192, 381, 1056, 1178, 1371
HgaI GACGC 1 cut(s) 821
HhaI GCGC 3 cut(s) 32, 218, 431
Hin1I GRCGYC 2 cut(s) 30, 281
Hin1II CATG 8 cut(s) 79, 109, 322, 542, 769, 825, 1040, 1404
Hin6I GCGC 3 cut(s) 30, 216, 429
HinP1I GCGC 3 cut(s) 30, 216, 429
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HinfI GANTC 5 cut(s) 5, 210, 264, 818, 906
HpaII CCGG 7 cut(s) 129, 161, 192, 381, 1056, 1178, 1371
HphI GGTGA 5 cut(s) 61, 250, 388, 644, 691
Hpy166II GTNNAC 3 cut(s) 378, 642, 739
Hpy188I TCNGA 5 cut(s) 10, 151, 303, 1075, 1410
Hpy188III TCNNGA 8 cut(s) 118, 362, 419, 659, 910, 975, 1138, 1312
Hpy8I GTNNAC 3 cut(s) 378, 642, 739
Hpy99I CGWCG 6 cut(s) 46, 175, 275, 283, 286, 869
HpyAV CCTTC 6 cut(s) 298, 359, 495, 513, 665, 1292
HpyCH4III ACNGT 5 cut(s) 238, 315, 593, 727, 848
HpyCH4IV ACGT 4 cut(s) 44, 281, 1386, 1447
HpyCH4V TGCA 8 cut(s) 322, 744, 837, 1036, 1052, 1273, 1346, 1414
HpyF10VI GCNNNNNNNGC 8 cut(s) 46, 55, 120, 186, 213, 328, 1343, 1346
HpyF3I CTNAG 2 cut(s) 1310, 1407
HpySE526I ACGT 4 cut(s) 44, 281, 1386, 1447
Hsp92I GRCGYC 2 cut(s) 30, 281
Hsp92II CATG 8 cut(s) 79, 109, 322, 542, 769, 825, 1040, 1404
HspAI GCGC 3 cut(s) 30, 216, 429
KasI GGCGCC 1 cut(s) 29
KpnI GGTACC 1 cut(s) 960
KspI CCGCGG 1 cut(s) 227
Kzo9I GATC 5 cut(s) 146, 451, 868, 895, 1192
LmnI GCTCC 8 cut(s) 63, 163, 177, 194, 449, 784, 796, 1267
Lsp1109I GCAGC 4 cut(s) 756, 1064, 1349, 1358
LweI GCATC 2 cut(s) 1349, 1401
MaeI CTAG 1 cut(s) 1334
MaeII ACGT 4 cut(s) 44, 281, 1386, 1447
MaeIII GTNAC 8 cut(s) 401, 650, 848, 1018, 1091, 1105, 1115, 1318
MalI GATC 5 cut(s) 148, 453, 870, 897, 1194
MbiI CCGCTC 1 cut(s) 158
MboI GATC 5 cut(s) 146, 451, 868, 895, 1192
MboII GAAGA 4 cut(s) 190, 244, 507, 854
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 3 cut(s) 146, 451, 1192
MhlI GDGCHC 1 cut(s) 349
MluCI AATT 5 cut(s) 435, 585, 709, 1045, 1361
Mly113I GGCGCC 1 cut(s) 30
MlyI GAGTC 2 cut(s) 219, 273
MmeI TCCRAC 2 cut(s) 961, 1418
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 5 cut(s) 390, 786, 882, 1067, 1247
MslI CAYNNNNRTG 4 cut(s) 74, 543, 938, 1124
MspA1I CMGCKG 1 cut(s) 226
MspI CCGG 7 cut(s) 129, 161, 192, 381, 1056, 1178, 1371
MspR9I CCNGG 5 cut(s) 130, 193, 449, 1057, 1178
MunI CAATTG 1 cut(s) 585
MvaI CCWGG 1 cut(s) 449
MvnI CGCG 2 cut(s) 218, 226
MwoI GCNNNNNNNGC 8 cut(s) 46, 55, 120, 186, 213, 328, 1343, 1346
NarI GGCGCC 1 cut(s) 30
NciI CCSGG 4 cut(s) 130, 193, 1057, 1178
NdeII GATC 5 cut(s) 146, 451, 868, 895, 1192
NlaIII CATG 8 cut(s) 79, 109, 322, 542, 769, 825, 1040, 1404
NmeAIII GCCGAG 1 cut(s) 420
NmuCI GTSAC 1 cut(s) 650
NspI RCATGY 2 cut(s) 322, 1040
PaeR7I CTCGAG 1 cut(s) 419
PcsI WCGNNNNNNNCGW 2 cut(s) 116, 242
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 3 cut(s) 5, 818, 906
PflFI GACNNNGTC 1 cut(s) 280
PkrI GCNGC 5 cut(s) 220, 746, 1054, 1339, 1348
PleI GAGTC 2 cut(s) 218, 272
PluTI GGCGCC 1 cut(s) 33
PpsI GAGTC 2 cut(s) 218, 272
PpuMI RGGWCCY 1 cut(s) 183
PshAI GACNNNNGTC 1 cut(s) 1437
PsiI TTATAA 1 cut(s) 758
Psp1406I AACGTT 1 cut(s) 1447
Psp5II RGGWCCY 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 447
PspEI GGTNACC 1 cut(s) 650
PspFI CCCAGC 1 cut(s) 123
PspGI CCWGG 1 cut(s) 447
PspOMI GGGCCC 1 cut(s) 345
PspPI GGNCC 6 cut(s) 112, 126, 183, 345, 346, 1164
PspPPI RGGWCCY 1 cut(s) 183
PstI CTGCAG 4 cut(s) 746, 1054, 1275, 1348
PstNI CAGNNNCTG 2 cut(s) 186, 1343
PsuI RGATCY 3 cut(s) 146, 451, 1192
PsyI GACNNNGTC 1 cut(s) 280
RsaI GTAC 4 cut(s) 317, 826, 958, 1269
RsaNI GTAC 4 cut(s) 316, 825, 957, 1268
RseI CAYNNNNRTG 4 cut(s) 74, 543, 938, 1124
SacII CCGCGG 1 cut(s) 227
SaqAI TTAA 5 cut(s) 390, 786, 882, 1067, 1247
SatI GCNGC 5 cut(s) 219, 745, 1053, 1338, 1347
Sau3AI GATC 5 cut(s) 146, 451, 868, 895, 1192
Sau96I GGNCC 6 cut(s) 112, 126, 183, 345, 346, 1164
ScaI AGTACT 1 cut(s) 1269
SchI GAGTC 2 cut(s) 219, 273
ScrFI CCNGG 5 cut(s) 130, 193, 449, 1057, 1178
SduI GDGCHC 1 cut(s) 349
SfaNI GCATC 2 cut(s) 1349, 1401
SfcI CTRYAG 4 cut(s) 742, 1050, 1271, 1344
SfiI GGCCNNNNNGGCC 1 cut(s) 46
SfoI GGCGCC 1 cut(s) 31
Sfr274I CTCGAG 1 cut(s) 419
Sfr303I CCGCGG 1 cut(s) 227
SgrBI CCGCGG 1 cut(s) 227
SinI GGWCC 2 cut(s) 183, 1164
SlaI CTCGAG 1 cut(s) 419
SmiMI CAYNNNNRTG 4 cut(s) 74, 543, 938, 1124
SmlI CTYRAG 2 cut(s) 419, 635
SmoI CTYRAG 2 cut(s) 419, 635
Sse9I AATT 5 cut(s) 435, 585, 709, 1045, 1361
SsiI CCGC 7 cut(s) 156, 207, 218, 224, 226, 331, 342
SspDI GGCGCC 1 cut(s) 29
SspMI CTAG 1 cut(s) 1334
StyD4I CCNGG 5 cut(s) 128, 191, 447, 1055, 1176
TaaI ACNGT 5 cut(s) 238, 315, 593, 727, 848
TaiI ACGT 4 cut(s) 47, 284, 1389, 1450
TaqI TCGA 5 cut(s) 245, 284, 420, 1084, 1281
TaqII GACCGA 1 cut(s) 124
TasI AATT 5 cut(s) 435, 585, 709, 1045, 1361
TatI WGTACW 2 cut(s) 824, 1267
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 3 cut(s) 5, 818, 906
Tru1I TTAA 5 cut(s) 390, 786, 882, 1067, 1247
Tru9I TTAA 5 cut(s) 390, 786, 882, 1067, 1247
TscAI CASTG 1 cut(s) 805
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 4 cut(s) 744, 1052, 1337, 1346
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 7 cut(s) 190, 527, 683, 719, 810, 1216, 1311
TspGWI ACGGA 1 cut(s) 305
TspRI CASTG 1 cut(s) 805
Tth111I GACNNNGTC 1 cut(s) 280
VpaK11BI GGWCC 2 cut(s) 183, 1164
XapI RAATTY 2 cut(s) 1045, 1361
XceI RCATGY 2 cut(s) 322, 1040
XhoI CTCGAG 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 641
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 1 cut(s) 1334
ZraI GACGTC 1 cut(s) 282
ZrmI AGTACT 1 cut(s) 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.