FvH4_1g24590

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0018360
Physical Location & Seq
Forward (+)
16424811 .. 16425766
956 bp
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UTR
Exon/CDS
Intron
FvH4_1g24590.t1

Sequence Viewer

Length: 723 bp
ATGGAAGATGACAACCATGGAAATAATAGGCTAAATGGTCCAAATGGAGGAAGCCCTGAAAACCCATGTTTAAACAACAGCACTCCTGCTAGTAGCTGCAACTCCAACATTAATATTCACCACAATCATAATCACAATAACAATCACAATCATCACCACCACAGCAGCAATAAAGAACAAGACAGGTTTCTTCCCATTGCCAATGTAGGTCGTATTATGAAGAAAGTGATTCCCGGCAATGGAAAAATCTCCAAAGACGCAAAAGAGACTGTCCAAGAATGTGTGTCTGAGTTCATTAGCTTTGTCACCGGAGAAGCCTCGGATAAATGTCAAAGGGAAAAGAGGAAAACCATCAACGGCGAAGACATCATATGGGCTATCACAACACTAGGGTTTGAGGAATACGTGCACCCTCTAAAATTGTACCTCCAAAAATATAGAGAGCTTGAAGGGGAGAAGCTAAATGTTCCAAAGCAACAAAGATTGGAGCAAAGGCTTCAGCAACAGCAACAACAGCAACAAGTAGTACAACAAGAACAAGAGCAACAAAGTATACCTAATTCGTATACTGTATATTCTTCGACGAGTCTCATGTCTCAACCGACTTCTTTCATTACTCATCATCATCATCATGATCCGGCATTTTCCTTGCCTTTCTCCCCAACCTCAATTCAGAAACAGTTACAGCCACAAGATCAGATTGATTCAGTGGGGCATTGGTAA

Protein Analysis

241

Amino Acids

27.47

Weight (kDa)

6.48

Isoelectric Point (pI)

56.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 51 - 127 2.4e-07 Core histone H2A/H2B/H3/H4 domain
CBFD_NFYB_HMF PF00808 63 - 127 6.1e-28 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24590
malus_domestica MD15G1134600.v1.1
prunus_persica Prupe.4G273800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0121991
rosa_laevigata RLG00000018645
rosa_roxburghii Rroxscaffold_2G00122090
rosa_rugosa Rorug02G0235900
rosa_samantha Rh2BG303000 Rh2CG281600
rosa_wichuraiana Rw2G023460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 553, 566
AclWI GGATC 1 cut(s) 629
AcuI CTGAAG 1 cut(s) 482
AfaI GTAC 2 cut(s) 425, 528
AfiI CCNNNNNNNGG 2 cut(s) 47, 239
AgsI TTSAA 1 cut(s) 449
AluBI AGCT 4 cut(s) 96, 300, 445, 460
AluI AGCT 4 cut(s) 96, 300, 445, 460
Alw21I GWGCWC 1 cut(s) 411
Alw26I GTCTC 3 cut(s) 260, 593, 600
Alw44I GTGCAC 1 cut(s) 407
AlwI GGATC 1 cut(s) 629
ApaLI GTGCAC 1 cut(s) 407
ApeKI GCWGC 2 cut(s) 96, 165
AseI ATTAAT 1 cut(s) 111
AspS9I GGNCC 1 cut(s) 38
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 3 cut(s) 110, 146, 298
AvaII GGWCC 1 cut(s) 38
BaeGI GKGCMC 1 cut(s) 411
BbsI GAAGAC 1 cut(s) 369
Bbv12I GWGCWC 1 cut(s) 411
BbvI GCAGC 2 cut(s) 83, 177
BccI CCATC 1 cut(s) 359
BceAI ACGGC 1 cut(s) 373
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 3 cut(s) 260, 593, 600
BfaI CTAG 2 cut(s) 90, 389
BisI GCNGC 2 cut(s) 97, 166
BlsI GCNGC 2 cut(s) 98, 167
Bme1390I CCNGG 1 cut(s) 234
Bme18I GGWCC 1 cut(s) 38
BmgT120I GGNCC 1 cut(s) 38
BmrFI CCNGG 1 cut(s) 234
BpiI GAAGAC 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 234
BsaAI YACGTR 1 cut(s) 406
BsaJI CCNNGG 2 cut(s) 16, 318
BsaWI WCCGGW 1 cut(s) 308
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 239
Bse3DI GCAATG 2 cut(s) 195, 244
BseDI CCNNGG 2 cut(s) 16, 318
BseLI CCNNNNNNNGG 2 cut(s) 47, 239
BseMI GCAATG 2 cut(s) 195, 244
BseMII CTCAG 1 cut(s) 279
BseSI GKGCMC 1 cut(s) 411
BseXI GCAGC 2 cut(s) 83, 177
BsiHKAI GWGCWC 1 cut(s) 411
BsiSI CCGG 3 cut(s) 234, 309, 638
BslI CCNNNNNNNGG 2 cut(s) 47, 239
BsmAI GTCTC 3 cut(s) 260, 593, 600
Bsp1286I GDGCHC 1 cut(s) 411
Bsp143I GATC 2 cut(s) 634, 694
Bsp19I CCATGG 1 cut(s) 16
BspCNI CTCAG 1 cut(s) 280
BspHI TCATGA 1 cut(s) 631
BspPI GGATC 1 cut(s) 629
BsrDI GCAATG 2 cut(s) 195, 244
BssECI CCNNGG 2 cut(s) 16, 318
BssMI GATC 2 cut(s) 634, 694
BssNAI GTATAC 2 cut(s) 554, 567
BssT1I CCWWGG 1 cut(s) 16
Bst1107I GTATAC 2 cut(s) 554, 567
Bst4CI ACNGT 3 cut(s) 271, 571, 681
BstBAI YACGTR 1 cut(s) 406
BstDEI CTNAG 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 16
BstKTI GATC 2 cut(s) 637, 697
BstMAI GTCTC 3 cut(s) 260, 593, 600
BstMBI GATC 2 cut(s) 634, 694
BstMWI GCNNNNNNNGC 1 cut(s) 514
BstSCI CCNGG 1 cut(s) 232
BstSLI GKGCMC 1 cut(s) 411
BstV1I GCAGC 2 cut(s) 83, 177
BstV2I GAAGAC 1 cut(s) 369
BstZ17I GTATAC 2 cut(s) 554, 567
BtgI CCRYGG 1 cut(s) 16
BtsIMutI CAGTG 1 cut(s) 714
CciI TCATGA 1 cut(s) 631
Cfr13I GGNCC 1 cut(s) 38
CseI GACGC 1 cut(s) 266
Csp6I GTAC 2 cut(s) 424, 527
CviAII CATG 4 cut(s) 17, 66, 592, 632
CviQI GTAC 2 cut(s) 424, 527
DdeI CTNAG 1 cut(s) 288
DpnI GATC 2 cut(s) 636, 696
DpnII GATC 2 cut(s) 634, 694
DraI TTTAAA 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 38
Eco57I CTGAAG 1 cut(s) 482
EcoT14I CCWWGG 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 16
FaeI CATG 4 cut(s) 20, 69, 595, 635
FatI CATG 4 cut(s) 16, 65, 591, 631
FauNDI CATATG 1 cut(s) 371
FblI GTMKAC 2 cut(s) 553, 566
Fnu4HI GCNGC 2 cut(s) 97, 166
Fsp4HI GCNGC 2 cut(s) 97, 166
FspBI CTAG 2 cut(s) 90, 389
GluI GCNGC 2 cut(s) 97, 166
HapII CCGG 3 cut(s) 234, 309, 638
HgaI GACGC 1 cut(s) 266
Hin1II CATG 4 cut(s) 20, 69, 595, 635
HinfI GANTC 3 cut(s) 229, 586, 704
HpaII CCGG 3 cut(s) 234, 309, 638
HphI GGTGA 3 cut(s) 110, 146, 298
Hpy166II GTNNAC 3 cut(s) 409, 554, 567
Hpy188I TCNGA 4 cut(s) 289, 322, 675, 699
Hpy188III TCNNGA 1 cut(s) 632
Hpy8I GTNNAC 3 cut(s) 409, 554, 567
Hpy99I CGWCG 1 cut(s) 586
HpyAV CCTTC 1 cut(s) 443
HpyCH4III ACNGT 3 cut(s) 271, 571, 681
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 2 cut(s) 99, 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 514
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 1 cut(s) 405
Hsp92II CATG 4 cut(s) 20, 69, 595, 635
Kzo9I GATC 2 cut(s) 634, 694
LmnI GCTCC 1 cut(s) 487
LpnPI CCDG 6 cut(s) 69, 99, 169, 247, 322, 651
Lsp1109I GCAGC 2 cut(s) 83, 177
MaeI CTAG 2 cut(s) 90, 389
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 2 cut(s) 304, 681
MalI GATC 2 cut(s) 636, 696
MboI GATC 2 cut(s) 634, 694
MboII GAAGA 5 cut(s) 17, 182, 232, 374, 570
MhlI GDGCHC 1 cut(s) 411
MluCI AATT 3 cut(s) 419, 559, 669
MlyI GAGTC 1 cut(s) 595
MmeI TCCRAC 1 cut(s) 129
MnlI CCTC 7 cut(s) 41, 328, 336, 391, 423, 437, 676
MseI TTAA 2 cut(s) 71, 111
MslI CAYNNNNRTG 1 cut(s) 630
MspI CCGG 3 cut(s) 234, 309, 638
MspR9I CCNGG 1 cut(s) 234
MssI GTTTAAAC 1 cut(s) 72
MwoI GCNNNNNNNGC 1 cut(s) 514
NciI CCSGG 1 cut(s) 234
NcoI CCATGG 1 cut(s) 16
NdeI CATATG 1 cut(s) 371
NdeII GATC 2 cut(s) 634, 694
NlaIII CATG 4 cut(s) 20, 69, 595, 635
NmuCI GTSAC 1 cut(s) 304
PagI TCATGA 1 cut(s) 631
PfeI GAWTC 2 cut(s) 229, 704
PkrI GCNGC 2 cut(s) 98, 167
PleI GAGTC 1 cut(s) 594
PmeI GTTTAAAC 1 cut(s) 72
PpsI GAGTC 1 cut(s) 594
Ppu21I YACGTR 1 cut(s) 406
PshBI ATTAAT 1 cut(s) 111
PspPI GGNCC 1 cut(s) 38
RsaI GTAC 2 cut(s) 425, 528
RsaNI GTAC 2 cut(s) 424, 527
RseI CAYNNNNRTG 1 cut(s) 630
SaqAI TTAA 2 cut(s) 71, 111
SatI GCNGC 2 cut(s) 97, 166
Sau3AI GATC 2 cut(s) 634, 694
Sau96I GGNCC 1 cut(s) 38
SchI GAGTC 1 cut(s) 595
ScrFI CCNGG 1 cut(s) 234
SduI GDGCHC 1 cut(s) 411
SinI GGWCC 1 cut(s) 38
SmiMI CAYNNNNRTG 1 cut(s) 630
Sse9I AATT 3 cut(s) 419, 559, 669
SspI AATATT 1 cut(s) 115
SspMI CTAG 2 cut(s) 90, 389
StyD4I CCNGG 1 cut(s) 232
StyI CCWWGG 1 cut(s) 16
TaaI ACNGT 3 cut(s) 271, 571, 681
TaiI ACGT 1 cut(s) 408
TaqI TCGA 1 cut(s) 581
TasI AATT 3 cut(s) 419, 559, 669
TatI WGTACW 1 cut(s) 526
TfiI GAWTC 2 cut(s) 229, 704
Tru1I TTAA 2 cut(s) 71, 111
Tru9I TTAA 2 cut(s) 71, 111
TscAI CASTG 1 cut(s) 714
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 2 cut(s) 96, 165
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 3 cut(s) 233, 283, 601
TspRI CASTG 1 cut(s) 714
VneI GTGCAC 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 38
VspI ATTAAT 1 cut(s) 111
XmiI GTMKAC 2 cut(s) 553, 566
XspI CTAG 2 cut(s) 90, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.