RLG00000018645

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018360
Physical Location & Seq
Forward (+)
33185086 .. 33185793
708 bp
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UTR
Exon/CDS
Intron
RLM00000018645

Sequence Viewer

Length: 708 bp
ATGGAAGATGACAACCATGGAAATAATAGGCTAAATGGTCCAAATGGAGGAAGCCCTGAAAACCCATGTTTAAACAACAGCACTCCTGCTAGTAGCTGCAACTCCAACATTAATATTCACCACGATCAGAATCACAGTAACAATCACAATCATCACCACCACAGCAGCAATAAAGAACAAGACAGGTTTCTTCCCATTGCGAATGTGGGTCGCATTATGAAGAAAGTGATTCCGGGCAATGGAAAAATCTCGAAAGATGCAAAAGAGACTGTCCAAGAATGTGTGTCCGAGTTCATTAGCTTTGTCACGGGTGAAGCCTCTGATAAATGTCAAAGGGAAAAGAGGAAGACCATCAACGGCGAAGACATCATATGGGCTATCACAACACTAGGCTTTGAGGATTACGTGCACCCTCTAAAATTGTACCTCCAAAAATATAGAGAGCTTGAAGGGGAGAAGCTAAATGTTCCAAAGCAACAAAGATTGGAGCAAAGGCTGCAGCAACAGCAAGTAGTACAGCAAGAACAAGAGCAACAAAGTATACATAATTCGTATAGTGTATATTCATCGACGAGTCTCATGTCTCAACCGACTTCTTTCATCACTACTCATCATCATGATCCCACATTTTCGTTGCCTTTCTCGCCAAGCTCAATTCAAAAACAGCTACAGCCACAAGATCAGATTGATTCAGTGGGGCATTGGTAA

Protein Analysis

236

Amino Acids

26.78

Weight (kDa)

6.3

Isoelectric Point (pI)

54.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 51 - 127 2.3e-07 Core histone H2A/H2B/H3/H4 domain
CBFD_NFYB_HMF PF00808 63 - 127 5.9e-28 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24590
malus_domestica MD15G1134600.v1.1
prunus_persica Prupe.4G273800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0121991
rosa_laevigata RLG00000018645
rosa_roxburghii Rroxscaffold_2G00122090
rosa_rugosa Rorug02G0235900
rosa_samantha Rh2BG303000 Rh2CG281600
rosa_wichuraiana Rw2G023460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 541
AclWI GGATC 1 cut(s) 614
AfaI GTAC 2 cut(s) 425, 516
AfiI CCNNNNNNNGG 2 cut(s) 47, 239
AgsI TTSAA 2 cut(s) 449, 659
AluBI AGCT 6 cut(s) 96, 300, 445, 460, 651, 667
AluI AGCT 6 cut(s) 96, 300, 445, 460, 651, 667
Alw21I GWGCWC 1 cut(s) 411
Alw26I GTCTC 3 cut(s) 260, 581, 588
Alw44I GTGCAC 1 cut(s) 407
AlwI GGATC 1 cut(s) 614
ApaLI GTGCAC 1 cut(s) 407
ApeKI GCWGC 4 cut(s) 96, 165, 496, 499
AseI ATTAAT 1 cut(s) 111
AspS9I GGNCC 1 cut(s) 38
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 3 cut(s) 110, 146, 323
AvaII GGWCC 1 cut(s) 38
BaeGI GKGCMC 1 cut(s) 411
BbsI GAAGAC 2 cut(s) 353, 369
Bbv12I GWGCWC 1 cut(s) 411
BbvI GCAGC 4 cut(s) 83, 177, 483, 511
BccI CCATC 1 cut(s) 359
BceAI ACGGC 1 cut(s) 373
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 3 cut(s) 260, 581, 588
BfaI CTAG 2 cut(s) 90, 389
BfmI CTRYAG 2 cut(s) 497, 668
BisI GCNGC 4 cut(s) 97, 166, 497, 500
BlsI GCNGC 4 cut(s) 98, 167, 498, 501
Bme1390I CCNGG 1 cut(s) 234
Bme18I GGWCC 1 cut(s) 38
BmgT120I GGNCC 1 cut(s) 38
BmrFI CCNGG 1 cut(s) 234
BmsI GCATC 1 cut(s) 247
BpiI GAAGAC 2 cut(s) 353, 369
BpuMI CCSGG 1 cut(s) 234
BsaAI YACGTR 1 cut(s) 406
BsaBI GATNNNNATC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 16
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 239
Bse3DI GCAATG 2 cut(s) 195, 244
Bse8I GATNNNNATC 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 16
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 2 cut(s) 47, 239
BseMI GCAATG 2 cut(s) 195, 244
BseSI GKGCMC 1 cut(s) 411
BseXI GCAGC 4 cut(s) 83, 177, 483, 511
BsiHKAI GWGCWC 1 cut(s) 411
BsiSI CCGG 1 cut(s) 233
BslI CCNNNNNNNGG 2 cut(s) 47, 239
BsmAI GTCTC 3 cut(s) 260, 581, 588
Bsp1286I GDGCHC 1 cut(s) 411
Bsp143I GATC 3 cut(s) 124, 619, 679
Bsp19I CCATGG 1 cut(s) 16
BspHI TCATGA 1 cut(s) 616
BspMAI CTGCAG 1 cut(s) 501
BspPI GGATC 1 cut(s) 614
BsrDI GCAATG 2 cut(s) 195, 244
BssECI CCNNGG 1 cut(s) 16
BssMI GATC 3 cut(s) 124, 619, 679
BssNAI GTATAC 1 cut(s) 542
BssT1I CCWWGG 1 cut(s) 16
Bst1107I GTATAC 1 cut(s) 542
Bst4CI ACNGT 2 cut(s) 137, 271
BstAPI GCANNNNNTGC 1 cut(s) 496
BstBAI YACGTR 1 cut(s) 406
BstDSI CCRYGG 1 cut(s) 16
BstKTI GATC 3 cut(s) 127, 622, 682
BstMAI GTCTC 3 cut(s) 260, 581, 588
BstMBI GATC 3 cut(s) 124, 619, 679
BstMWI GCNNNNNNNGC 3 cut(s) 496, 505, 643
BstSCI CCNGG 1 cut(s) 232
BstSFI CTRYAG 2 cut(s) 497, 668
BstSLI GKGCMC 1 cut(s) 411
BstV1I GCAGC 4 cut(s) 83, 177, 483, 511
BstV2I GAAGAC 2 cut(s) 353, 369
BstZ17I GTATAC 1 cut(s) 542
BtgI CCRYGG 1 cut(s) 16
BtsIMutI CAGTG 1 cut(s) 699
CciI TCATGA 1 cut(s) 616
Cfr13I GGNCC 1 cut(s) 38
Csp6I GTAC 2 cut(s) 424, 515
CviAII CATG 4 cut(s) 17, 66, 580, 617
CviQI GTAC 2 cut(s) 424, 515
DpnI GATC 3 cut(s) 126, 621, 681
DpnII GATC 3 cut(s) 124, 619, 679
DraI TTTAAA 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 16
FaeI CATG 4 cut(s) 20, 69, 583, 620
FatI CATG 4 cut(s) 16, 65, 579, 616
FauNDI CATATG 1 cut(s) 371
FblI GTMKAC 1 cut(s) 541
Fnu4HI GCNGC 4 cut(s) 97, 166, 497, 500
Fsp4HI GCNGC 4 cut(s) 97, 166, 497, 500
FspBI CTAG 2 cut(s) 90, 389
GluI GCNGC 4 cut(s) 97, 166, 497, 500
HapII CCGG 1 cut(s) 233
Hin1II CATG 4 cut(s) 20, 69, 583, 620
HinfI GANTC 4 cut(s) 130, 229, 574, 689
HpaII CCGG 1 cut(s) 233
HphI GGTGA 3 cut(s) 110, 146, 323
Hpy166II GTNNAC 2 cut(s) 409, 542
Hpy188I TCNGA 4 cut(s) 129, 289, 322, 684
Hpy188III TCNNGA 2 cut(s) 250, 617
Hpy8I GTNNAC 2 cut(s) 409, 542
Hpy99I CGWCG 1 cut(s) 574
HpyAV CCTTC 1 cut(s) 443
HpyCH4III ACNGT 2 cut(s) 137, 271
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 4 cut(s) 99, 260, 409, 499
HpyF10VI GCNNNNNNNGC 3 cut(s) 496, 505, 643
HpySE526I ACGT 1 cut(s) 405
Hsp92II CATG 4 cut(s) 20, 69, 583, 620
Kzo9I GATC 3 cut(s) 124, 619, 679
LmnI GCTCC 1 cut(s) 487
LpnPI CCDG 4 cut(s) 69, 99, 169, 246
Lsp1109I GCAGC 4 cut(s) 83, 177, 483, 511
LweI GCATC 1 cut(s) 247
MaeI CTAG 2 cut(s) 90, 389
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 2 cut(s) 137, 304
MalI GATC 3 cut(s) 126, 621, 681
MboI GATC 3 cut(s) 124, 619, 679
MboII GAAGA 5 cut(s) 17, 182, 232, 358, 374
MhlI GDGCHC 1 cut(s) 411
MluCI AATT 3 cut(s) 419, 547, 654
MlyI GAGTC 1 cut(s) 583
MmeI TCCRAC 1 cut(s) 129
MnlI CCTC 6 cut(s) 41, 328, 336, 391, 423, 437
MseI TTAA 2 cut(s) 71, 111
MslI CAYNNNNRTG 1 cut(s) 615
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 1 cut(s) 234
MssI GTTTAAAC 1 cut(s) 72
MwoI GCNNNNNNNGC 3 cut(s) 496, 505, 643
NciI CCSGG 1 cut(s) 234
NcoI CCATGG 1 cut(s) 16
NdeI CATATG 1 cut(s) 371
NdeII GATC 3 cut(s) 124, 619, 679
NlaIII CATG 4 cut(s) 20, 69, 583, 620
NmuCI GTSAC 1 cut(s) 304
PagI TCATGA 1 cut(s) 616
PfeI GAWTC 3 cut(s) 130, 229, 689
PkrI GCNGC 4 cut(s) 98, 167, 498, 501
PleI GAGTC 1 cut(s) 582
PmeI GTTTAAAC 1 cut(s) 72
PpsI GAGTC 1 cut(s) 582
Ppu21I YACGTR 1 cut(s) 406
PshBI ATTAAT 1 cut(s) 111
PspPI GGNCC 1 cut(s) 38
PstI CTGCAG 1 cut(s) 501
RsaI GTAC 2 cut(s) 425, 516
RsaNI GTAC 2 cut(s) 424, 515
RseI CAYNNNNRTG 1 cut(s) 615
SaqAI TTAA 2 cut(s) 71, 111
SatI GCNGC 4 cut(s) 97, 166, 497, 500
Sau3AI GATC 3 cut(s) 124, 619, 679
Sau96I GGNCC 1 cut(s) 38
SchI GAGTC 1 cut(s) 583
ScrFI CCNGG 1 cut(s) 234
SduI GDGCHC 1 cut(s) 411
SetI ASST 9 cut(s) 98, 188, 302, 408, 429, 447, 462, 653, 669
SfaNI GCATC 1 cut(s) 247
SfcI CTRYAG 2 cut(s) 497, 668
SinI GGWCC 1 cut(s) 38
SmiMI CAYNNNNRTG 1 cut(s) 615
Sse9I AATT 3 cut(s) 419, 547, 654
SspI AATATT 1 cut(s) 115
SspMI CTAG 2 cut(s) 90, 389
StyD4I CCNGG 1 cut(s) 232
StyI CCWWGG 1 cut(s) 16
TaaI ACNGT 2 cut(s) 137, 271
TaiI ACGT 1 cut(s) 408
TaqI TCGA 2 cut(s) 251, 569
TasI AATT 3 cut(s) 419, 547, 654
TatI WGTACW 1 cut(s) 514
TfiI GAWTC 3 cut(s) 130, 229, 689
Tru1I TTAA 2 cut(s) 71, 111
Tru9I TTAA 2 cut(s) 71, 111
TscAI CASTG 1 cut(s) 699
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 4 cut(s) 96, 165, 496, 499
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 4 cut(s) 233, 283, 555, 589
TspRI CASTG 1 cut(s) 699
VneI GTGCAC 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 38
VspI ATTAAT 1 cut(s) 111
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XmiI GTMKAC 1 cut(s) 541
XspI CTAG 2 cut(s) 90, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.