Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0006543

Physical Location & Seq

Genomic Coordinates Forward (+)

17769480 .. 17775976

Gene Structure 6497 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g25850.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2349 bp

ATGGATCCAGAAAAGGAGAGCAGCAACCAAGTCCGTGTTGGTATTGCTGACATTCAGAACTTTGATTTGTTGGAGAATAAGGAGGGTGAGAATAATGAGAACAGTAGGACACACAGCTACAAGGAACCGCTTCTGACCAGAAGGATCACCAACACAACATCTCAGATAGCCATCGTTGGTTCCAATGTCTGCCCCATTGAGAGCCTTGACTATGAGATTTTTGAAAACGATCTATTTAAGCAGGATTGGAGGTCAGCCAAGAAGAGTCGAATTCTTAAGTATGTGGTGCTAAAGTGGGCATTGGCACTTGTCATTGGGCTTGCAACTGGCCTAGTTGGCTTCTTCAATAATATTGCTGTCGAGAATATTGCTGGATACAAGTTGCTGTTGACCAGCAGTTTCATGGCAAAGGAAGAATATTACAAGGCTTTTGCAGTATATGCTTCCATGAATGTAGGTCTAGCAACAGCTGCTGCAGCTCTTTGTGCTTTCATTGCTCCAGCAGCAGCAGGTTCTGGTATTCCGGAGGTCAAAGCTTATCTAAATGGAATAGATGCATATTCCATACTGGCTCCAAGCACCCTTTTTGTGAAGATTTTTGGTTCCATTCTTGGTGTTTCTGCCGGATTTGTGGTTGGTAAAGAAGGCCCCATGGTACATACAGGTGCTTGCATAGCCTCTTTGCTTGGGCAGGGAGGTTCTCGCAAGTATCATTTGACCTGGTCGTGGCTAAGATACTTCAAAAATGATCGTGACCGACGAGACTTGATCACATGTGGTGCTGCAGCTGGTGTGGCTGCTGCCTTTCGTGCTCCAGTTGGCGGTGTTCTCTTTGCCCTTGAAGAAGCTGCTTCATGGTGGCGGAGTGCACTTCTTTGGAGAACATTTTTCACAACAGCAGTAGTCGCAATAGTACTGAGAGGTTTCATACAGTACTGTGCTACAGGAGACTGTGGCCTATTTGGGCAAGGCGGTCTGATTATGTACGACGTCAGTGCAGCTGATGCAACATATAGCGCTCCGGATATACTAGCGGTATTACTTCTTGGTGTTTTTGGAGGTATTTTTGGAAGCATATACAACTATCTTGTTGACAAGGTCATCCGAACTTACAGCATCATTCATGAAAAGGGTACAGCTCCAAAAATCTTGCTTGTTATCACCATTGCCCTATTGACGTCATCCGTTTCTTATGGCTTGCCATGGTTTTCAACGTGCATTGCATGCCCCACTGATGCGACTGTCAGTTGTCCTACTGTTGGTAAAGCTGGCAACTATATAAGCTTCAACTGCCCAACAGGCTATTACAATGACATTGCCTCCCTCTTTCTGAACACTAACGATGATGCTATTCGTAATCTATTTAGCACTGATACAACAAAAGAATTCCACATATACTCTCTTTTCATCTTCTTTTCTGCTGTTTTCATCCTTGGAATAATTACATATGGCATTGCTATTCCCTCTGGGCTTTTCATCCCCGTCATACTAGCCGGAGCTTGCTATGGTCGTCTTGTTGGCAGGTTGTTTGAATCAATATCCAGTCTCGACACAGGCCTCTTTGCTCTACTTGGAGCTGCCTCGTTACTTGGTGGCACTATGCGGATGACAGTTTCCCTATGTATCATATTGCTTGAACTGACCAATGACTTGCTGTTGCTTCCACTGGTGATGTTGGTACTGCTTATTTCAAAAACTGTGGCTGATAACTTCAACAAGGGAATTTATGACCAAATAGTTAAGATAAAGGGATTGCCTTATTTGGAGGCTCATGCAGAACCTTACATGAAGGATTTGGTCACAAGTGATGTTGTTTCAGGTCCATTGGTTACCTTTTCCGGTGTTGAGAAGGTAGGCTATATTATGCATGCTCTGAGGACTACAGGGCATAATGGATTCCCAGTGATTGATGAACCACCTTTCTCAGACACTCCAGAGTTGTGTGGTCTTGTTTTAAGGTCCCATTTACTTACTTTGCTCAATGGTAAGGTCTTTTCAAAGGAGAGGATTCTTCATGGAGAAGAGATCTTACGCAGATTTGGTCCATTTGATTTTGCTAAGGCTGGATCAGGAAAGGGACTCAAAGTAGAAGATCTTGATATAGAAGAGGAAGAGATGGAAATGTATGTTGACCTCCATCCTATTACAAATGCATCCCCATACACTGTGGTTGAGACAATGTCACTAGCGAAAGCTGCTATTCTGTTTCGACAACTTGGTCTCAGGCACTTATGTTTGGTACCAAAGAGCCAAGGGAGATCTCCAATTGTAGGAATTCTGACTCGGCATGACTTCATGCCAGAGCACATTTTGGGACTCTACCCTAACATCAAGCCTCACAACAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005247 GO:0005253 GO:0005254 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006821 GO:0008150 GO:0008308 GO:0008509 GO:0009705 GO:0015075 GO:0015103 GO:0015108 GO:0015267 GO:0015318 GO:0015698 GO:0016020 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0098588 GO:0098656 GO:0098660 GO:0098661 GO:0098805 GO:1902476

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

783

Amino Acids

85.15

Weight (kDa)

6.23

Isoelectric Point (pI)

35.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	156 - 569	1.3e-76	Voltage gated chloride channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006543)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49890
fragaria_vesca	FvH4_1g25850 FvH4_1g25850
malus_domestica	MD00G1030600.v1.1
prunus_persica	Prupe.1G583800_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1
pyrus_communis	pycom08g21410 pycom520g01280
rosa_chinensis	RchiOBHm_Chr3g0484991
rosa_laevigata	RLG00000023179
rosa_multiflora	Rmu_sc0006040.1_g000024 Rmu_sc0009462.1_g000005
rosa_roxburghii	Rroxscaffold_6G00397280
rosa_rugosa	Rorug03G0214900.1 Rorug03G0215000
rosa_samantha	Rh3AG265300 Rh3CG298700 Rh3DG294600
rosa_wichuraiana	Rw0G006760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2217
AatII	GACGTC	2 cut(s)	993, 1181
Acc36I	ACCTGC	2 cut(s)	500, 1512
Acc65I	GGTACC	1 cut(s)	2239
AccB1I	GGYRCC	1 cut(s)	2239
AccIII	TCCGGA	2 cut(s)	523, 1021
AciI	CCGC	6 cut(s)	128, 822, 862, 972, 1034, 1603
AclWI	GGATC	3 cut(s)	12, 152, 2074
AcsI	RAATTY	4 cut(s)	270, 1385, 1722, 2274
AcyI	GRCGYC	2 cut(s)	990, 1178
AfaI	GTAC	7 cut(s)	657, 915, 935, 986, 1135, 1680, 2241
AfeI	AGCGCT	1 cut(s)	1018
AfiI	CCNNNNNNNGG	4 cut(s)	726, 821, 1259, 2270
AflII	CTTAAG	1 cut(s)	275
AflIII	ACRYGT	1 cut(s)	773
AjnI	CCWGG	1 cut(s)	719
Alw21I	GWGCWC	3 cut(s)	814, 871, 2307
Alw26I	GTCTC	5 cut(s)	756, 942, 1550, 2168, 2225
Alw44I	GTGCAC	1 cut(s)	867
AlwI	GGATC	3 cut(s)	12, 152, 2074
AlwNI	CAGNNNCTG	2 cut(s)	473, 515
Aor13HI	TCCGGA	2 cut(s)	523, 1021
Aor51HI	AGCGCT	1 cut(s)	1018
AoxI	GGCC	4 cut(s)	328, 646, 955, 1555
ApaLI	GTGCAC	1 cut(s)	867
ApoI	RAATTY	4 cut(s)	270, 1385, 1722, 2274
Asp700I	GAANNNNTTC	1 cut(s)	129
Asp718I	GGTACC	1 cut(s)	2239
AspLEI	GCGC	1 cut(s)	1019
AspS9I	GGNCC	4 cut(s)	647, 1820, 1959, 2042
AsuHPI	GGTGA	4 cut(s)	98, 139, 1153, 1681
AvaII	GGWCC	3 cut(s)	1820, 1959, 2042
BaeGI	GKGCMC	1 cut(s)	871
BamHI	GGATCC	1 cut(s)	4
BanI	GGYRCC	1 cut(s)	2239
BarI	GAAGNNNNNNTAC	2 cut(s)	2013, 2045
Bbv12I	GWGCWC	3 cut(s)	814, 871, 2307
BccI	CCATC	3 cut(s)	179, 2110, 2145
BcgI	CGANNNNNNTGC	4 cut(s)	350, 384, 977, 1011
BciT130I	CCWGG	1 cut(s)	721
BciVI	GTATCC	1 cut(s)	368
BclI	TGATCA	1 cut(s)	768
BcoDI	GTCTC	5 cut(s)	756, 942, 1550, 2168, 2225
BfaI	CTAG	6 cut(s)	332, 461, 1031, 1490, 2186, 2347
BfmI	CTRYAG	4 cut(s)	474, 783, 942, 1881
BfoI	RGCGCY	1 cut(s)	1020
BfrI	CTTAAG	1 cut(s)	275
BfuAI	ACCTGC	2 cut(s)	500, 1512
BfuI	GTATCC	1 cut(s)	368
BglI	GCCNNNNNGGC	2 cut(s)	336, 1299
BglII	AGATCT	3 cut(s)	2025, 2092, 2258
BmcAI	AGTACT	2 cut(s)	915, 935
Bme1390I	CCNGG	1 cut(s)	721
Bme18I	GGWCC	3 cut(s)	1820, 1959, 2042
BmgT120I	GGNCC	4 cut(s)	647, 1820, 1959, 2042
BmiI	GGNNCC	8 cut(s)	6, 126, 181, 573, 604, 649, 1961, 2241
BmrFI	CCNGG	1 cut(s)	721
BmrI	ACTGGG	1 cut(s)	1895
BmsI	GCATC	6 cut(s)	544, 994, 1125, 1225, 1336, 2162
BmuI	ACTGGG	1 cut(s)	1895
BpmI	CTGGAG	3 cut(s)	483, 798, 1917
Bpu10I	CCTNAGC	1 cut(s)	2058
BsaBI	GATNNNNATC	1 cut(s)	170
BsaHI	GRCGYC	2 cut(s)	990, 1178
BsaI	GGTCTC	1 cut(s)	2225
BsaJI	CCNNGG	4 cut(s)	651, 1202, 1432, 2251
BsaWI	WCCGGW	3 cut(s)	523, 1021, 1838
BsaXI	ACNNNNNCTCC	2 cut(s)	1304, 1334
Bsc4I	CCNNNNNNNGG	4 cut(s)	726, 821, 1259, 2270
Bse1I	ACTGG	6 cut(s)	331, 573, 815, 1542, 1671, 1901
Bse3DI	GCAATG	5 cut(s)	492, 1164, 1218, 1314, 1452
Bse8I	GATNNNNATC	1 cut(s)	170
BseAI	TCCGGA	2 cut(s)	523, 1021
BseBI	CCWGG	1 cut(s)	721
BseDI	CCNNGG	4 cut(s)	651, 1202, 1432, 2251
BseGI	GGATG	7 cut(s)	1101, 1181, 1428, 1476, 1611, 2137, 2153
BseJI	GATNNNNATC	1 cut(s)	170
BseLI	CCNNNNNNNGG	4 cut(s)	726, 821, 1259, 2270
BseMI	GCAATG	5 cut(s)	492, 1164, 1218, 1314, 1452
BseMII	CTCAG	5 cut(s)	176, 908, 1865, 1938, 2236
BseNI	ACTGG	6 cut(s)	331, 573, 815, 1542, 1671, 1901
BseSI	GKGCMC	1 cut(s)	871
BsgI	GTGCAG	1 cut(s)	1017
BshFI	GGCC	4 cut(s)	330, 648, 957, 1557
BshNI	GGYRCC	1 cut(s)	2239
BsiHKAI	GWGCWC	3 cut(s)	814, 871, 2307
BsiSI	CCGG	5 cut(s)	524, 624, 1022, 1494, 1839
BslFI	GGGAC	3 cut(s)	1945, 2091, 2328
BslI	CCNNNNNNNGG	4 cut(s)	726, 821, 1259, 2270
BsmAI	GTCTC	5 cut(s)	756, 942, 1550, 2168, 2225
BsmFI	GGGAC	3 cut(s)	1945, 2091, 2328
BsnI	GGCC	4 cut(s)	330, 648, 957, 1557
Bso31I	GGTCTC	1 cut(s)	2225
Bsp1286I	GDGCHC	3 cut(s)	814, 871, 2307
Bsp13I	TCCGGA	2 cut(s)	523, 1021
Bsp143I	GATC	9 cut(s)	4, 144, 229, 748, 768, 2025, 2066, 2092, 2258
Bsp19I	CCATGG	2 cut(s)	651, 1202
BspACI	CCGC	6 cut(s)	128, 822, 862, 972, 1034, 1603
BspANI	GGCC	4 cut(s)	330, 648, 957, 1557
BspCNI	CTCAG	5 cut(s)	175, 909, 1866, 1937, 2235
BspEI	TCCGGA	2 cut(s)	523, 1021
BspHI	TCATGA	1 cut(s)	1123
BspLI	GGNNCC	8 cut(s)	6, 126, 181, 573, 604, 649, 1961, 2241
BspMAI	CTGCAG	2 cut(s)	478, 787
BspMI	ACCTGC	2 cut(s)	500, 1512
BspPI	GGATC	3 cut(s)	12, 152, 2074
BspT107I	GGYRCC	1 cut(s)	2239
BspTI	CTTAAG	1 cut(s)	275
BspTNI	GGTCTC	1 cut(s)	2225
BsrDI	GCAATG	5 cut(s)	492, 1164, 1218, 1314, 1452
BsrI	ACTGG	6 cut(s)	331, 573, 815, 1542, 1671, 1901
BssECI	CCNNGG	4 cut(s)	651, 1202, 1432, 2251
BssMI	GATC	9 cut(s)	4, 144, 229, 748, 768, 2025, 2066, 2092, 2258
BssNI	GRCGYC	2 cut(s)	990, 1178
BssT1I	CCWWGG	4 cut(s)	651, 1202, 1432, 2251
Bst2UI	CCWGG	1 cut(s)	721
Bst4CI	ACNGT	9 cut(s)	104, 933, 938, 953, 1243, 1258, 1612, 1699, 2167
Bst6I	CTCTTC	4 cut(s)	257, 2016, 2100, 2106
BstACI	GRCGYC	2 cut(s)	990, 1178
BstAFI	CTTAAG	1 cut(s)	275
BstAPI	GCANNNNNTGC	4 cut(s)	440, 470, 1004, 1224
BstC8I	GCNNGC	7 cut(s)	321, 670, 1199, 1225, 1270, 1501, 1869
BstDEI	CTNAG	7 cut(s)	162, 731, 917, 1874, 1924, 2058, 2222
BstDSI	CCRYGG	2 cut(s)	651, 1202
BstEII	GGTNACC	1 cut(s)	1828
BstF5I	GGATG	7 cut(s)	1101, 1181, 1428, 1476, 1611, 2137, 2153
BstH2I	RGCGCY	1 cut(s)	1020
BstHHI	GCGC	1 cut(s)	1019
BstKTI	GATC	9 cut(s)	7, 147, 232, 751, 771, 2028, 2069, 2095, 2261
BstMAI	GTCTC	5 cut(s)	756, 942, 1550, 2168, 2225
BstMBI	GATC	9 cut(s)	4, 144, 229, 748, 768, 2025, 2066, 2092, 2258
BstNI	CCWGG	1 cut(s)	721
BstNSI	RCATGY	3 cut(s)	777, 1227, 1871
BstPI	GGTNACC	1 cut(s)	1828
BstSCI	CCNGG	1 cut(s)	719
BstSFI	CTRYAG	4 cut(s)	474, 783, 942, 1881
BstSLI	GKGCMC	1 cut(s)	871
BstX2I	RGATCY	4 cut(s)	4, 2025, 2092, 2258
BstYI	RGATCY	4 cut(s)	4, 2025, 2092, 2258
BsuI	GTATCC	1 cut(s)	368
BsuRI	GGCC	4 cut(s)	330, 648, 957, 1557
BtgI	CCRYGG	2 cut(s)	651, 1202
BtsCI	GGATG	7 cut(s)	1101, 1181, 1428, 1476, 1611, 2137, 2153
BtsIMutI	CAGTG	6 cut(s)	1000, 1230, 1368, 1664, 1908, 2163
BveI	ACCTGC	2 cut(s)	500, 1512
Cac8I	GCNNGC	7 cut(s)	321, 670, 1199, 1225, 1270, 1501, 1869
CaiI	CAGNNNCTG	2 cut(s)	473, 515
CciI	TCATGA	1 cut(s)	1123
CfoI	GCGC	1 cut(s)	1019
Cfr13I	GGNCC	4 cut(s)	647, 1820, 1959, 2042
CsiI	ACCWGGT	1 cut(s)	719
Csp6I	GTAC	7 cut(s)	656, 914, 934, 985, 1134, 1679, 2240
CspCI	CAANNNNNGTGG	2 cut(s)	1680, 1715
CviQI	GTAC	7 cut(s)	656, 914, 934, 985, 1134, 1679, 2240
DdeI	CTNAG	7 cut(s)	162, 731, 917, 1874, 1924, 2058, 2222
DpnI	GATC	9 cut(s)	6, 146, 231, 750, 770, 2027, 2068, 2094, 2260
DpnII	GATC	9 cut(s)	4, 144, 229, 748, 768, 2025, 2066, 2092, 2258
DrdI	GACNNNNNNGTC	1 cut(s)	2217
DseDI	GACNNNNNNGTC	1 cut(s)	2217
Eam1104I	CTCTTC	4 cut(s)	257, 2016, 2100, 2106
EarI	CTCTTC	4 cut(s)	257, 2016, 2100, 2106
EciI	GGCGGA	1 cut(s)	877
Eco130I	CCWWGG	4 cut(s)	651, 1202, 1432, 2251
Eco147I	AGGCCT	1 cut(s)	1557
Eco31I	GGTCTC	1 cut(s)	2225
Eco47I	GGWCC	3 cut(s)	1820, 1959, 2042
Eco47III	AGCGCT	1 cut(s)	1018
Eco91I	GGTNACC	1 cut(s)	1828
EcoO109I	RGGNCCY	2 cut(s)	647, 1959
EcoO65I	GGTNACC	1 cut(s)	1828
EcoRI	GAATTC	3 cut(s)	270, 1385, 2274
EcoRII	CCWGG	1 cut(s)	719
EcoT14I	CCWWGG	4 cut(s)	651, 1202, 1432, 2251
EcoT22I	ATGCAT	3 cut(s)	559, 1869, 2155
ErhI	CCWWGG	4 cut(s)	651, 1202, 1432, 2251
FaqI	GGGAC	3 cut(s)	1945, 2091, 2328
FauNDI	CATATG	1 cut(s)	1447
FbaI	TGATCA	1 cut(s)	768
FokI	GGATG	7 cut(s)	1088, 1168, 1415, 1463, 1618, 2124, 2140
FspBI	CTAG	6 cut(s)	332, 461, 1031, 1490, 2186, 2347
GlaI	GCGC	1 cut(s)	1018
GsuI	CTGGAG	3 cut(s)	483, 798, 1917
HaeII	RGCGCY	1 cut(s)	1020
HaeIII	GGCC	4 cut(s)	330, 648, 957, 1557
HapII	CCGG	5 cut(s)	524, 624, 1022, 1494, 1839
HhaI	GCGC	1 cut(s)	1019
Hin1I	GRCGYC	2 cut(s)	990, 1178
Hin6I	GCGC	1 cut(s)	1017
HinP1I	GCGC	1 cut(s)	1017
HincII	GTYRAC	3 cut(s)	390, 1093, 2131
HindII	GTYRAC	3 cut(s)	390, 1093, 2131
HindIII	AAGCTT	2 cut(s)	534, 1282
HinfI	GANTC	7 cut(s)	265, 1532, 1896, 2008, 2079, 2281, 2316
HpaII	CCGG	5 cut(s)	524, 624, 1022, 1494, 1839
HphI	GGTGA	4 cut(s)	98, 139, 1153, 1681
Hpy166II	GTNNAC	4 cut(s)	390, 869, 1093, 2131
Hpy188I	TCNGA	9 cut(s)	57, 135, 165, 978, 1106, 1332, 1875, 1927, 2280
Hpy8I	GTNNAC	4 cut(s)	390, 869, 1093, 2131
Hpy99I	CGWCG	2 cut(s)	762, 992
HpyAV	CCTTC	4 cut(s)	135, 638, 1783, 1843
HpyCH4III	ACNGT	9 cut(s)	104, 933, 938, 953, 1243, 1258, 1612, 1699, 2167
HpyCH4IV	ACGT	3 cut(s)	990, 1178, 1214
HpyF3I	CTNAG	7 cut(s)	162, 731, 917, 1874, 1924, 2058, 2222
HpySE526I	ACGT	3 cut(s)	990, 1178, 1214
Hsp92I	GRCGYC	2 cut(s)	990, 1178
HspAI	GCGC	1 cut(s)	1017
Kpn2I	TCCGGA	2 cut(s)	523, 1021
KpnI	GGTACC	1 cut(s)	2243
Ksp22I	TGATCA	1 cut(s)	768
Kzo9I	GATC	9 cut(s)	4, 144, 229, 748, 768, 2025, 2066, 2092, 2258
LmnI	GCTCC	7 cut(s)	502, 577, 817, 1024, 1144, 1496, 1574
LweI	GCATC	6 cut(s)	544, 994, 1125, 1225, 1336, 2162
MabI	ACCWGGT	1 cut(s)	719
MaeI	CTAG	6 cut(s)	332, 461, 1031, 1490, 2186, 2347
MaeII	ACGT	3 cut(s)	990, 1178, 1214
MaeIII	GTNAC	5 cut(s)	752, 1584, 1798, 1828, 2181
MalI	GATC	9 cut(s)	6, 146, 231, 750, 770, 2027, 2068, 2094, 2260
MboI	GATC	9 cut(s)	4, 144, 229, 748, 768, 2025, 2066, 2092, 2258
MfeI	CAATTG	1 cut(s)	2265
MflI	RGATCY	4 cut(s)	4, 2025, 2092, 2258
MhlI	GDGCHC	3 cut(s)	814, 871, 2307
MluCI	AATT	6 cut(s)	270, 1385, 1440, 1722, 2265, 2274
MlyI	GAGTC	4 cut(s)	274, 2073, 2275, 2310
MmeI	TCCRAC	1 cut(s)	51
Mph1103I	ATGCAT	3 cut(s)	559, 1869, 2155
MroI	TCCGGA	2 cut(s)	523, 1021
MroXI	GAANNNNTTC	1 cut(s)	129
MseI	TTAA	4 cut(s)	237, 276, 1740, 1955
MslI	CAYNNNNRTG	1 cut(s)	663
MspA1I	CMGCKG	3 cut(s)	470, 788, 1001
MspCI	CTTAAG	1 cut(s)	275
MspI	CCGG	5 cut(s)	524, 624, 1022, 1494, 1839
MspR9I	CCNGG	1 cut(s)	721
MunI	CAATTG	1 cut(s)	2265
MvaI	CCWGG	1 cut(s)	721
NcoI	CCATGG	2 cut(s)	651, 1202
NdeI	CATATG	1 cut(s)	1447
NdeII	GATC	9 cut(s)	4, 144, 229, 748, 768, 2025, 2066, 2092, 2258
NlaIV	GGNNCC	8 cut(s)	6, 126, 181, 573, 604, 649, 1961, 2241
NmeAIII	GCCGAG	1 cut(s)	2263
NmuCI	GTSAC	3 cut(s)	752, 1798, 2181
NsiI	ATGCAT	3 cut(s)	559, 1869, 2155
NspI	RCATGY	3 cut(s)	777, 1227, 1871
PaeI	GCATGC	2 cut(s)	1227, 1871
PagI	TCATGA	1 cut(s)	1123
PceI	AGGCCT	1 cut(s)	1557
PciI	ACATGT	1 cut(s)	773
PcsI	WCGNNNNNNNCGW	1 cut(s)	757
PdmI	GAANNNNTTC	1 cut(s)	129
PfeI	GAWTC	3 cut(s)	1532, 1896, 2008
PflFI	GACNNNGTC	3 cut(s)	721, 1097, 2179
PleI	GAGTC	4 cut(s)	273, 2073, 2275, 2310
PpsI	GAGTC	4 cut(s)	273, 2073, 2275, 2310
PpuMI	RGGWCCY	1 cut(s)	1959
PscI	ACATGT	1 cut(s)	773
Psp5II	RGGWCCY	1 cut(s)	1959
Psp6I	CCWGG	1 cut(s)	719
PspEI	GGTNACC	1 cut(s)	1828
PspGI	CCWGG	1 cut(s)	719
PspN4I	GGNNCC	8 cut(s)	6, 126, 181, 573, 604, 649, 1961, 2241
PspPI	GGNCC	4 cut(s)	647, 1820, 1959, 2042
PspPPI	RGGWCCY	1 cut(s)	1959
PstI	CTGCAG	2 cut(s)	478, 787
PstNI	CAGNNNCTG	2 cut(s)	473, 515
PsuI	RGATCY	4 cut(s)	4, 2025, 2092, 2258
PsyI	GACNNNGTC	3 cut(s)	721, 1097, 2179
PvuII	CAGCTG	3 cut(s)	470, 788, 1001
RsaI	GTAC	7 cut(s)	657, 915, 935, 986, 1135, 1680, 2241
RsaNI	GTAC	7 cut(s)	656, 914, 934, 985, 1134, 1679, 2240
RseI	CAYNNNNRTG	1 cut(s)	663
SaqAI	TTAA	4 cut(s)	237, 276, 1740, 1955
Sau3AI	GATC	9 cut(s)	4, 144, 229, 748, 768, 2025, 2066, 2092, 2258
Sau96I	GGNCC	4 cut(s)	647, 1820, 1959, 2042
ScaI	AGTACT	2 cut(s)	915, 935
SchI	GAGTC	4 cut(s)	274, 2073, 2275, 2310
ScrFI	CCNGG	1 cut(s)	721
SduI	GDGCHC	3 cut(s)	814, 871, 2307
SexAI	ACCWGGT	1 cut(s)	719
SfaNI	GCATC	6 cut(s)	544, 994, 1125, 1225, 1336, 2162
SfcI	CTRYAG	4 cut(s)	474, 783, 942, 1881
SinI	GGWCC	3 cut(s)	1820, 1959, 2042
SmiMI	CAYNNNNRTG	1 cut(s)	663
SmlI	CTYRAG	1 cut(s)	275
SmoI	CTYRAG	1 cut(s)	275
SphI	GCATGC	2 cut(s)	1227, 1871
Sse9I	AATT	6 cut(s)	270, 1385, 1440, 1722, 2265, 2274
SseBI	AGGCCT	1 cut(s)	1557
SsiI	CCGC	6 cut(s)	128, 822, 862, 972, 1034, 1603
SspI	AATATT	3 cut(s)	352, 367, 419
SspMI	CTAG	6 cut(s)	332, 461, 1031, 1490, 2186, 2347
StuI	AGGCCT	1 cut(s)	1557
StyD4I	CCNGG	1 cut(s)	719
StyI	CCWWGG	4 cut(s)	651, 1202, 1432, 2251
TaaI	ACNGT	9 cut(s)	104, 933, 938, 953, 1243, 1258, 1612, 1699, 2167
TaiI	ACGT	3 cut(s)	993, 1181, 1217
TaqI	TCGA	4 cut(s)	268, 360, 1548, 2209
TaqII	GACCGA	1 cut(s)	771
TasI	AATT	6 cut(s)	270, 1385, 1440, 1722, 2265, 2274
TatI	WGTACW	2 cut(s)	913, 933
TfiI	GAWTC	3 cut(s)	1532, 1896, 2008
Tru1I	TTAA	4 cut(s)	237, 276, 1740, 1955
Tru9I	TTAA	4 cut(s)	237, 276, 1740, 1955
TscAI	CASTG	6 cut(s)	1000, 1237, 1375, 1671, 1908, 2170
TseFI	GTSAC	3 cut(s)	752, 1798, 2181
Tsp45I	GTSAC	3 cut(s)	752, 1798, 2181
TspGWI	ACGGA	2 cut(s)	23, 1174
TspRI	CASTG	6 cut(s)	1000, 1237, 1375, 1671, 1908, 2170
Tth111I	GACNNNGTC	3 cut(s)	721, 1097, 2179
Vha464I	CTTAAG	1 cut(s)	275
VneI	GTGCAC	1 cut(s)	867
VpaK11BI	GGWCC	3 cut(s)	1820, 1959, 2042
XapI	RAATTY	4 cut(s)	270, 1385, 1722, 2274
XceI	RCATGY	3 cut(s)	777, 1227, 1871
XcmI	CCANNNNNNNNNTGG	2 cut(s)	35, 400
XmnI	GAANNNNTTC	1 cut(s)	129
XspI	CTAG	6 cut(s)	332, 461, 1031, 1490, 2186, 2347
ZraI	GACGTC	2 cut(s)	991, 1179
ZrmI	AGTACT	2 cut(s)	915, 935
Zsp2I	ATGCAT	3 cut(s)	559, 1869, 2155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.