Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0006543

Physical Location & Seq

Genomic Coordinates Forward (+)

20891520 .. 20895996

Gene Structure 4477 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g21410.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2277 bp

ATGGAATTGGAGGAGGAAAAGAGTATAGAAATAGAACGAGGGGGGTTGAGGGAGCCACTGCTTGAGAGGAATTGGAGGAGAAACACGACCTCTCAGCTTGCCATTGTTGGTGCCAATCTCTCTCCCATTGAAAGCCTTGACTACGAGATTATTGAGAATGATCTTTTTAAGCAGGACTGGAGGTCTAGAACGAAGGGTGAGATATTTCAGTACGTTTGCCTCAAATGGGCACTTGTGCTGCTTATTGGTTTAAGCACTGGGTTAGTCGGCTTCTTCAATAACCTAGCTGTCGAGAATATAGCTGGTTTCAAACTTCTGCTAGCCAACAACCTCATGCTTCAGGACAAGTATTATCAGGCATTTGCAGCATTCGTTTGTTGTAACATGACATTGGCTGCTGCTGCTGCAGCTCTTTGTGCTTACATTGCCCCGGCAGCAGCAGGCTCTGGCATACCTGAGGTTAAAGCATATCTAAATGGAGTCGATGCTCATTCTATATTGGCTCCAAGTACCCTCTTTGTAAAGATTTTTGGTTCTATATTTGGAGTAGCTGCTGGGTTTATTTTGGGTAAGGAAGGACCTATGGTTCACTCTGGCGCTTGCATAGCCTCTTTGCTTGGACAGGTTGGCTCTCGCAAGTATCACCTTACCTGGAAATGTCTCAGGTTCTACAAAAATGACCGTGACCGAAGGGATCTGATTACCTGCGGTGCTGCTGCTGGTGTTGCAGCTGCCTTCCGTGCCCCAGTTGGTGGGGTCCTTTTTGCGCTAGAAGAAGCAGCTTCATGGTGGAGGAGTGCTCTTCTGTGGAGGGCATTTTTCACAACAGCTGTAGTAGCGGTACTGTTAAGAGGCTTCATACAGTTATGCAAGGGTGGAAAATGTGGGCTATTCGGGGAAGGAGGTCTTATAATGTTTGATGTCAATTCTGCAAAGGTCAGTTATAGCAGCGGCGATCTACTGGCAGTCATATCCCTTGGAGTTATCGGAGGCATATTTGGAAGCCTTTACAATTTCCTTGTGGACAAAGTCCTTCAAACTTATCGCATCATAAACAAGAGTGGTCCTTCAAGTAAAATTCTGCTCGCCGTTGCCATTTCCTTTTTGACTTCTTGTTGCTCTTACGGCCTCCCATGGCTTTCACACTGCATTCCTTGTCCCCGTCACCTGGAGGACAAGTGCCCCACCATAGGTCGCTCTGGAAACTACAAGAATTTTCAGTGTCCACCTAACCACTACAATGGCCTTGCTTCACTCTTCTTCAATACCAATGATAATGCTATCCGCAACCTTTTAAGCTCTAGCTCTGATGGGGAGTTCCGGCTTTCCACTCTCATTGTTTTCTTTGTTACCGTGTACTGCCTGGGCATTGTTACTTATGGTATTGCTGTTCCCTCTGGGCTCTTCATCCCTGTTATACTTGCTGGGTCATCCTATGGGCGTGTAGTCGGGACCCTCCTTGGTTCTCACTCTGATCTTGATGGGGGTTTCTTTGCACTCCTTGGAGCGGCATCCTTCCTTGGTGGCACCATGAGAATGACTGTTTCTCTCTGTGTCATACTTCTTGAGCTTACAAATAATCTTCTAATGCTTCCACTGATGATGCTAGTTCTGCTTATTTCGAAATCTGTGGCGGATTGTTTCAACAAGGGTGTCTATGACCAAATTATAAAAATGAAGGGATTACCGTATATGGAGGCGCATGCAGAACCATACATGAGAAACTTGGTTGCAAGTGATGTTGTTTCTTGGCCCTTAATTTCATTTTCCAGGGTTGAGAAAGTGGGGAATATATTACACGAGCTCAAAACAACAGCGCATAATGGGTTCCCCGTGATTGATGAACCACCTTTCTCAAATTCTGCAGACTTGTGTGGGCTGGTTTTGAGATCGCATTTGCTTGTATTGCTTAAACGAAAGAATTTTACAAAGCAAAGGGTGTTGATTGGGTCAGATGTTACGAGTAGGTTCAGAGCACATGATTTTGGAAAGGCAGGATTAGGCAAGGGAATCAAGCTGGAGGATTTGGACATTCAGGAGGAAGAGATGGAAATGTATCTTGACCTCCATCCCATTACTAATACCTCCCCATACACCGTAGTTGAAACAATGTCCCTTGCTAAAGCTGCTATTTTCTTTCGGGCGCTTGGCCTCAGGCACTTGCTGGTGGTGCCTAAGACACCAGGGAGGCCTCCAATTGTTGGGATCTTGACAAGGCACGATTTCATGCCGGAGCACATATTGGAACTTTTTCCGCAGTTGAATCCTCACGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005247 GO:0005253 GO:0005254 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006821 GO:0008150 GO:0008308 GO:0008509 GO:0009705 GO:0015075 GO:0015103 GO:0015108 GO:0015267 GO:0015318 GO:0015698 GO:0016020 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0098588 GO:0098656 GO:0098660 GO:0098661 GO:0098805 GO:1902476

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

759

Amino Acids

82.88

Weight (kDa)

8.39

Isoelectric Point (pI)

36.56

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006543)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49890
fragaria_vesca	FvH4_1g25850 FvH4_1g25850
malus_domestica	MD00G1030600.v1.1
prunus_persica	Prupe.1G583800_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1
pyrus_communis	pycom08g21410 pycom520g01280
rosa_chinensis	RchiOBHm_Chr3g0484991
rosa_laevigata	RLG00000023179
rosa_multiflora	Rmu_sc0006040.1_g000024 Rmu_sc0009462.1_g000005
rosa_roxburghii	Rroxscaffold_6G00397280
rosa_rugosa	Rorug03G0214900.1 Rorug03G0215000
rosa_samantha	Rh3AG265300 Rh3CG298700 Rh3DG294600
rosa_wichuraiana	Rw0G006760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	911, 1670
Acc36I	ACCTGC	1 cut(s)	713
AccB1I	GGYRCC	3 cut(s)	110, 1526, 2170
AccB7I	CCANNNNNTGG	2 cut(s)	752, 2201
AccBSI	CCGCTC	1 cut(s)	1508
AciI	CCGC	7 cut(s)	708, 839, 951, 1285, 1508, 1634, 2255
AclWI	GGATC	2 cut(s)	702, 2213
AcsI	RAATTY	4 cut(s)	1077, 1213, 1858, 1921
AcuI	CTGAAG	1 cut(s)	323
AfaI	GTAC	4 cut(s)	212, 511, 843, 1358
AfiI	CCNNNNNNNGG	6 cut(s)	226, 752, 1168, 1190, 1507, 2201
AgsI	TTSAA	9 cut(s)	131, 277, 310, 1037, 1071, 1264, 1645, 2105, 2263
AhdI	GACNNNNNGTC	1 cut(s)	181
AjnI	CCWGG	5 cut(s)	650, 1167, 1362, 1769, 2182
AloI	GAACNNNNNNTCC	2 cut(s)	570, 602
Alw21I	GWGCWC	4 cut(s)	802, 1806, 1978, 2238
Alw26I	GTCTC	1 cut(s)	665
AlwI	GGATC	2 cut(s)	702, 2213
AlwNI	CAGNNNCTG	1 cut(s)	446
AoxI	GGCC	5 cut(s)	1126, 1243, 1751, 2149, 2189
ApoI	RAATTY	4 cut(s)	1077, 1213, 1858, 1921
Asp700I	GAANNNNTTC	1 cut(s)	2250
AspLEI	GCGC	5 cut(s)	599, 769, 1702, 1819, 2146
AspS9I	GGNCC	5 cut(s)	578, 757, 1064, 1452, 1752
AsuC2I	CCSGG	1 cut(s)	431
AsuHPI	GGTGA	3 cut(s)	209, 635, 1157
AsuII	TTCGAA	1 cut(s)	1622
AsuNHI	GCTAGC	1 cut(s)	319
AvaII	GGWCC	4 cut(s)	578, 757, 1064, 1452
AxyI	CCTNAGG	2 cut(s)	456, 2153
BaeGI	GKGCMC	3 cut(s)	232, 745, 1184
BanI	GGYRCC	3 cut(s)	110, 1526, 2170
BanII	GRGCYC	2 cut(s)	1404, 1806
BauI	CACGAG	1 cut(s)	1799
Bbv12I	GWGCWC	4 cut(s)	802, 1806, 1978, 2238
BccI	CCATC	4 cut(s)	1304, 1475, 2041, 2076
BceAI	ACGGC	2 cut(s)	1073, 1141
BciT130I	CCWGG	5 cut(s)	652, 1169, 1364, 1771, 2184
BcnI	CCSGG	1 cut(s)	431
BcoDI	GTCTC	1 cut(s)	665
BfaI	CTAG	6 cut(s)	186, 284, 320, 770, 1302, 1607
BfmI	CTRYAG	3 cut(s)	405, 831, 1863
BfoI	RGCGCY	2 cut(s)	600, 2147
BfuAI	ACCTGC	1 cut(s)	713
Bme1390I	CCNGG	6 cut(s)	431, 652, 1169, 1364, 1771, 2184
Bme18I	GGWCC	4 cut(s)	578, 757, 1064, 1452
BmeRI	GACNNNNNGTC	1 cut(s)	181
BmgT120I	GGNCC	5 cut(s)	578, 757, 1064, 1452, 1752
BmiI	GGNNCC	9 cut(s)	54, 112, 504, 758, 1453, 1454, 1528, 1829, 2172
BmrFI	CCNGG	6 cut(s)	431, 652, 1169, 1364, 1771, 2184
BmrI	ACTGGG	2 cut(s)	267, 740
BmsI	GCATC	4 cut(s)	475, 1056, 1520, 1593
BmtI	GCTAGC	1 cut(s)	323
BmuI	ACTGGG	2 cut(s)	267, 740
BplI	GAGNNNNNCTC	2 cut(s)	784, 816
BpmI	CTGGAG	3 cut(s)	199, 1190, 2039
Bpu14I	TTCGAA	1 cut(s)	1622
BpuEI	CTTGAG	2 cut(s)	83, 1586
BpuMI	CCSGG	1 cut(s)	431
BsaJI	CCNNGG	9 cut(s)	429, 976, 1133, 1363, 1459, 1501, 1519, 1770, 2183
BsaXI	ACNNNNNCTCC	2 cut(s)	1163, 1193
Bsc4I	CCNNNNNNNGG	6 cut(s)	226, 752, 1168, 1190, 1507, 2201
Bse1I	ACTGG	4 cut(s)	182, 262, 746, 966
Bse21I	CCTNAGG	2 cut(s)	456, 2153
Bse3DI	GCAATG	1 cut(s)	423
BseBI	CCWGG	5 cut(s)	652, 1169, 1364, 1771, 2184
BseDI	CCNNGG	9 cut(s)	429, 976, 1133, 1363, 1459, 1501, 1519, 1770, 2183
BseGI	GGATG	4 cut(s)	1407, 1430, 1511, 2068
BseLI	CCNNNNNNNGG	6 cut(s)	226, 752, 1168, 1190, 1507, 2201
BseMI	GCAATG	1 cut(s)	423
BseMII	CTCAG	4 cut(s)	107, 447, 676, 2167
BseNI	ACTGG	4 cut(s)	182, 262, 746, 966
BseRI	GAGGAG	3 cut(s)	26, 91, 808
BseSI	GKGCMC	3 cut(s)	232, 745, 1184
BseYI	CCCAGC	2 cut(s)	554, 1424
BshFI	GGCC	5 cut(s)	1128, 1245, 1753, 2151, 2191
BshNI	GGYRCC	3 cut(s)	110, 1526, 2170
BsiHKAI	GWGCWC	4 cut(s)	802, 1806, 1978, 2238
BsiSI	CCGG	3 cut(s)	431, 1321, 2231
BslFI	GGGAC	3 cut(s)	1143, 1465, 2098
BslI	CCNNNNNNNGG	6 cut(s)	226, 752, 1168, 1190, 1507, 2201
BsmAI	GTCTC	1 cut(s)	665
BsmFI	GGGAC	3 cut(s)	1143, 1465, 2098
BsmI	GAATGC	2 cut(s)	368, 1149
BsnI	GGCC	5 cut(s)	1128, 1245, 1753, 2151, 2191
Bsp119I	TTCGAA	1 cut(s)	1622
Bsp1286I	GDGCHC	8 cut(s)	232, 745, 802, 1184, 1404, 1806, 1978, 2238
Bsp143I	GATC	6 cut(s)	160, 694, 955, 1474, 1889, 2205
Bsp19I	CCATGG	1 cut(s)	1133
BspACI	CCGC	7 cut(s)	708, 839, 951, 1285, 1508, 1634, 2255
BspANI	GGCC	5 cut(s)	1128, 1245, 1753, 2151, 2191
BspCNI	CTCAG	4 cut(s)	106, 448, 675, 2166
BspLI	GGNNCC	9 cut(s)	54, 112, 504, 758, 1453, 1454, 1528, 1829, 2172
BspMAI	CTGCAG	2 cut(s)	409, 1867
BspMI	ACCTGC	1 cut(s)	713
BspOI	GCTAGC	1 cut(s)	323
BspPI	GGATC	2 cut(s)	702, 2213
BspQI	GCTCTTC	2 cut(s)	807, 1409
BspT104I	TTCGAA	1 cut(s)	1622
BspT107I	GGYRCC	3 cut(s)	110, 1526, 2170
BsrBI	CCGCTC	1 cut(s)	1508
BsrDI	GCAATG	1 cut(s)	423
BsrI	ACTGG	4 cut(s)	182, 262, 746, 966
BssECI	CCNNGG	9 cut(s)	429, 976, 1133, 1363, 1459, 1501, 1519, 1770, 2183
BssMI	GATC	6 cut(s)	160, 694, 955, 1474, 1889, 2205
BssSI	CACGAG	1 cut(s)	1799
BssT1I	CCWWGG	5 cut(s)	976, 1133, 1459, 1501, 1519
Bst2BI	CACGAG	1 cut(s)	1799
Bst2UI	CCWGG	5 cut(s)	652, 1169, 1364, 1771, 2184
Bst4CI	ACNGT	7 cut(s)	683, 846, 864, 1354, 1543, 1689, 2098
Bst6I	CTCTTC	4 cut(s)	807, 1262, 1409, 2037
BstBI	TTCGAA	1 cut(s)	1622
BstC8I	GCNNGC	6 cut(s)	99, 321, 442, 601, 1086, 1704
BstDEI	CTNAG	5 cut(s)	93, 456, 662, 2153, 2175
BstDSI	CCRYGG	1 cut(s)	1133
BstF5I	GGATG	4 cut(s)	1407, 1430, 1511, 2068
BstH2I	RGCGCY	2 cut(s)	600, 2147
BstHHI	GCGC	5 cut(s)	599, 769, 1702, 1819, 2146
BstKTI	GATC	6 cut(s)	163, 697, 958, 1477, 1892, 2208
BstMAI	GTCTC	1 cut(s)	665
BstMBI	GATC	6 cut(s)	160, 694, 955, 1474, 1889, 2205
BstNI	CCWGG	5 cut(s)	652, 1169, 1364, 1771, 2184
BstNSI	RCATGY	1 cut(s)	1706
BstSCI	CCNGG	6 cut(s)	429, 650, 1167, 1362, 1769, 2182
BstSFI	CTRYAG	3 cut(s)	405, 831, 1863
BstSLI	GKGCMC	3 cut(s)	232, 745, 1184
BstX2I	RGATCY	2 cut(s)	694, 2205
BstXI	CCANNNNNNTGG	1 cut(s)	1241
BstYI	RGATCY	2 cut(s)	694, 2205
Bsu36I	CCTNAGG	2 cut(s)	456, 2153
BsuRI	GGCC	5 cut(s)	1128, 1245, 1753, 2151, 2191
BtgI	CCRYGG	1 cut(s)	1133
BtsCI	GGATG	4 cut(s)	1407, 1430, 1511, 2068
BtsI	GCAGTG	2 cut(s)	56, 1144
BtsIMutI	CAGTG	5 cut(s)	56, 255, 1144, 1226, 1595
BveI	ACCTGC	1 cut(s)	713
Cac8I	GCNNGC	6 cut(s)	99, 321, 442, 601, 1086, 1704
CaiI	CAGNNNCTG	1 cut(s)	446
CfoI	GCGC	5 cut(s)	599, 769, 1702, 1819, 2146
Cfr13I	GGNCC	5 cut(s)	578, 757, 1064, 1452, 1752
Csp6I	GTAC	4 cut(s)	211, 510, 842, 1357
CspCI	CAANNNNNGTGG	2 cut(s)	1318, 1353
CviAII	CATG	9 cut(s)	334, 385, 786, 1134, 1531, 1703, 1717, 1979, 2227
CviQI	GTAC	4 cut(s)	211, 510, 842, 1357
DdeI	CTNAG	5 cut(s)	93, 456, 662, 2153, 2175
DpnI	GATC	6 cut(s)	162, 696, 957, 1476, 1891, 2207
DpnII	GATC	6 cut(s)	160, 694, 955, 1474, 1889, 2205
DriI	GACNNNNNGTC	1 cut(s)	181
Eam1104I	CTCTTC	4 cut(s)	807, 1262, 1409, 2037
Eam1105I	GACNNNNNGTC	1 cut(s)	181
EarI	CTCTTC	4 cut(s)	807, 1262, 1409, 2037
EciI	GGCGGA	1 cut(s)	1649
Ecl136II	GAGCTC	1 cut(s)	1804
Eco130I	CCWWGG	5 cut(s)	976, 1133, 1459, 1501, 1519
Eco147I	AGGCCT	1 cut(s)	2191
Eco24I	GRGCYC	2 cut(s)	1404, 1806
Eco47I	GGWCC	4 cut(s)	578, 757, 1064, 1452
Eco53kI	GAGCTC	1 cut(s)	1804
Eco57I	CTGAAG	1 cut(s)	323
Eco81I	CCTNAGG	2 cut(s)	456, 2153
EcoICRI	GAGCTC	1 cut(s)	1804
EcoO109I	RGGNCCY	3 cut(s)	578, 757, 1452
EcoRII	CCWGG	5 cut(s)	650, 1167, 1362, 1769, 2182
EcoT14I	CCWWGG	5 cut(s)	976, 1133, 1459, 1501, 1519
EcoT38I	GRGCYC	2 cut(s)	1404, 1806
ErhI	CCWWGG	5 cut(s)	976, 1133, 1459, 1501, 1519
FaeI	CATG	9 cut(s)	337, 388, 789, 1137, 1534, 1706, 1720, 1982, 2230
FalI	AAGNNNNNCTT	2 cut(s)	891, 923
FaqI	GGGAC	3 cut(s)	1143, 1465, 2098
FatI	CATG	9 cut(s)	333, 384, 785, 1133, 1530, 1702, 1716, 1978, 2226
FokI	GGATG	4 cut(s)	1394, 1417, 1498, 2055
FriOI	GRGCYC	2 cut(s)	1404, 1806
FspBI	CTAG	6 cut(s)	186, 284, 320, 770, 1302, 1607
GlaI	GCGC	5 cut(s)	598, 768, 1701, 1818, 2145
GsaI	CCCAGC	2 cut(s)	558, 1428
GsuI	CTGGAG	3 cut(s)	199, 1190, 2039
HaeII	RGCGCY	2 cut(s)	600, 2147
HaeIII	GGCC	5 cut(s)	1128, 1245, 1753, 2151, 2191
HapII	CCGG	3 cut(s)	431, 1321, 2231
HhaI	GCGC	5 cut(s)	599, 769, 1702, 1819, 2146
Hin1II	CATG	9 cut(s)	337, 388, 789, 1137, 1534, 1706, 1720, 1982, 2230
Hin6I	GCGC	5 cut(s)	597, 767, 1700, 1817, 2144
HinP1I	GCGC	5 cut(s)	597, 767, 1700, 1817, 2144
HinfI	GANTC	3 cut(s)	480, 2010, 2263
HpaII	CCGG	3 cut(s)	431, 1321, 2231
HphI	GGTGA	3 cut(s)	209, 635, 1157
Hpy166II	GTNNAC	4 cut(s)	589, 1024, 1226, 1357
Hpy188I	TCNGA	6 cut(s)	699, 989, 1309, 1474, 1954, 1973
Hpy8I	GTNNAC	4 cut(s)	589, 1024, 1226, 1357
HpyAV	CCTTC	9 cut(s)	187, 569, 684, 745, 893, 1043, 1077, 1525, 1672
HpyCH4III	ACNGT	7 cut(s)	683, 846, 864, 1354, 1543, 1689, 2098
HpyCH4IV	ACGT	1 cut(s)	213
HpyF3I	CTNAG	5 cut(s)	93, 456, 662, 2153, 2175
HpySE526I	ACGT	1 cut(s)	213
Hsp92II	CATG	9 cut(s)	337, 388, 789, 1137, 1534, 1706, 1720, 1982, 2230
HspAI	GCGC	5 cut(s)	597, 767, 1700, 1817, 2144
KflI	GGGWCCC	1 cut(s)	1452
Kzo9I	GATC	6 cut(s)	160, 694, 955, 1474, 1889, 2205
LguI	GCTCTTC	2 cut(s)	807, 1409
LmnI	GCTCC	4 cut(s)	52, 508, 1505, 2233
LweI	GCATC	4 cut(s)	475, 1056, 1520, 1593
MaeI	CTAG	6 cut(s)	186, 284, 320, 770, 1302, 1607
MaeII	ACGT	1 cut(s)	213
MaeIII	GTNAC	6 cut(s)	380, 683, 1163, 1348, 1372, 1957
MalI	GATC	6 cut(s)	162, 696, 957, 1476, 1891, 2207
MbiI	CCGCTC	1 cut(s)	1508
MboI	GATC	6 cut(s)	160, 694, 955, 1474, 1889, 2205
MboII	GAAGA	8 cut(s)	265, 785, 794, 1249, 1252, 1396, 1574, 2054
MfeI	CAATTG	1 cut(s)	2196
MflI	RGATCY	2 cut(s)	694, 2205
MhlI	GDGCHC	8 cut(s)	232, 745, 802, 1184, 1404, 1806, 1978, 2238
MlyI	GAGTC	1 cut(s)	489
MroXI	GAANNNNTTC	1 cut(s)	2250
MseI	TTAA	7 cut(s)	168, 251, 462, 848, 1295, 1757, 1911
MslI	CAYNNNNRTG	2 cut(s)	1239, 1535
MspA1I	CMGCKG	3 cut(s)	731, 830, 951
MspI	CCGG	3 cut(s)	431, 1321, 2231
MspR9I	CCNGG	6 cut(s)	431, 652, 1169, 1364, 1771, 2184
MunI	CAATTG	1 cut(s)	2196
Mva1269I	GAATGC	2 cut(s)	368, 1149
MvaI	CCWGG	5 cut(s)	652, 1169, 1364, 1771, 2184
NciI	CCSGG	1 cut(s)	431
NcoI	CCATGG	1 cut(s)	1133
NdeII	GATC	6 cut(s)	160, 694, 955, 1474, 1889, 2205
NheI	GCTAGC	1 cut(s)	319
NlaIII	CATG	9 cut(s)	337, 388, 789, 1137, 1534, 1706, 1720, 1982, 2230
NlaIV	GGNNCC	9 cut(s)	54, 112, 504, 758, 1453, 1454, 1528, 1829, 2172
NmuCI	GTSAC	2 cut(s)	683, 1163
NspI	RCATGY	1 cut(s)	1706
NspV	TTCGAA	1 cut(s)	1622
PaeI	GCATGC	1 cut(s)	1706
PceI	AGGCCT	1 cut(s)	2191
PciSI	GCTCTTC	2 cut(s)	807, 1409
PctI	GAATGC	2 cut(s)	368, 1149
PdmI	GAANNNNTTC	1 cut(s)	2250
PfeI	GAWTC	2 cut(s)	2010, 2263
PflFI	GACNNNGTC	1 cut(s)	1028
PflMI	CCANNNNNTGG	2 cut(s)	752, 2201
PleI	GAGTC	1 cut(s)	488
PpsI	GAGTC	1 cut(s)	488
PpuMI	RGGWCCY	3 cut(s)	578, 757, 1452
PsiI	TTATAA	2 cut(s)	911, 1670
Psp124BI	GAGCTC	1 cut(s)	1806
Psp5II	RGGWCCY	3 cut(s)	578, 757, 1452
Psp6I	CCWGG	5 cut(s)	650, 1167, 1362, 1769, 2182
PspFI	CCCAGC	2 cut(s)	554, 1424
PspGI	CCWGG	5 cut(s)	650, 1167, 1362, 1769, 2182
PspN4I	GGNNCC	9 cut(s)	54, 112, 504, 758, 1453, 1454, 1528, 1829, 2172
PspPI	GGNCC	5 cut(s)	578, 757, 1064, 1452, 1752
PspPPI	RGGWCCY	3 cut(s)	578, 757, 1452
PstI	CTGCAG	2 cut(s)	409, 1867
PstNI	CAGNNNCTG	1 cut(s)	446
PsuI	RGATCY	2 cut(s)	694, 2205
PsyI	GACNNNGTC	1 cut(s)	1028
PvuII	CAGCTG	2 cut(s)	731, 830
RsaI	GTAC	4 cut(s)	212, 511, 843, 1358
RsaNI	GTAC	4 cut(s)	211, 510, 842, 1357
RseI	CAYNNNNRTG	2 cut(s)	1239, 1535
SacI	GAGCTC	1 cut(s)	1806
SapI	GCTCTTC	2 cut(s)	807, 1409
SaqAI	TTAA	7 cut(s)	168, 251, 462, 848, 1295, 1757, 1911
Sau3AI	GATC	6 cut(s)	160, 694, 955, 1474, 1889, 2205
Sau96I	GGNCC	5 cut(s)	578, 757, 1064, 1452, 1752
SchI	GAGTC	1 cut(s)	489
ScrFI	CCNGG	6 cut(s)	431, 652, 1169, 1364, 1771, 2184
SduI	GDGCHC	8 cut(s)	232, 745, 802, 1184, 1404, 1806, 1978, 2238
SfaNI	GCATC	4 cut(s)	475, 1056, 1520, 1593
SfcI	CTRYAG	3 cut(s)	405, 831, 1863
SfuI	TTCGAA	1 cut(s)	1622
SinI	GGWCC	4 cut(s)	578, 757, 1064, 1452
SmiMI	CAYNNNNRTG	2 cut(s)	1239, 1535
SmlI	CTYRAG	2 cut(s)	62, 1565
SmoI	CTYRAG	2 cut(s)	62, 1565
SphI	GCATGC	1 cut(s)	1706
SseBI	AGGCCT	1 cut(s)	2191
SsiI	CCGC	7 cut(s)	708, 839, 951, 1285, 1508, 1634, 2255
SspMI	CTAG	6 cut(s)	186, 284, 320, 770, 1302, 1607
SstI	GAGCTC	1 cut(s)	1806
StuI	AGGCCT	1 cut(s)	2191
StyD4I	CCNGG	6 cut(s)	429, 650, 1167, 1362, 1769, 2182
StyI	CCWWGG	5 cut(s)	976, 1133, 1459, 1501, 1519
TaaI	ACNGT	7 cut(s)	683, 846, 864, 1354, 1543, 1689, 2098
TaiI	ACGT	1 cut(s)	216
TaqI	TCGA	3 cut(s)	291, 483, 1622
TaqII	GACCGA	1 cut(s)	702
TatI	WGTACW	1 cut(s)	1356
TauI	GCSGC	2 cut(s)	954, 1511
TfiI	GAWTC	2 cut(s)	2010, 2263
Tru1I	TTAA	7 cut(s)	168, 251, 462, 848, 1295, 1757, 1911
Tru9I	TTAA	7 cut(s)	168, 251, 462, 848, 1295, 1757, 1911
TscAI	CASTG	5 cut(s)	63, 262, 1151, 1226, 1602
TseFI	GTSAC	2 cut(s)	683, 1163
Tsp45I	GTSAC	2 cut(s)	683, 1163
TspDTI	ATGAA	7 cut(s)	774, 847, 1396, 1691, 1752, 1857, 2215
TspGWI	ACGGA	1 cut(s)	728
TspRI	CASTG	5 cut(s)	63, 262, 1151, 1226, 1602
Tth111I	GACNNNGTC	1 cut(s)	1028
Van91I	CCANNNNNTGG	2 cut(s)	752, 2201
VpaK11BI	GGWCC	4 cut(s)	578, 757, 1064, 1452
XapI	RAATTY	4 cut(s)	1077, 1213, 1858, 1921
XbaI	TCTAGA	1 cut(s)	185
XceI	RCATGY	1 cut(s)	1706
XmnI	GAANNNNTTC	1 cut(s)	2250
XspI	CTAG	6 cut(s)	186, 284, 320, 770, 1302, 1607

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.