Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0006543

Physical Location & Seq

Genomic Coordinates Reverse (-)

4948430 .. 4952791

Gene Structure 4362 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1030600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGATGAATGAAGAAAATGGAAGCAGCAGCAGCAGTAGCAGCAGCTTGGTGAGTGACGATGGTGGCGACATTGAGAATTTGGATTTGTTGGATGGAAGGAGGAGGACATGTAGCCAACCACTTCTTAGCAAGAGGAAGAACACTACTTCTCAGATTGCCATTGTCGGTTCCAATCTCTGCTCCATCGAAAGCCTTGACTACGAGATGTTTGAGAATGAAATATTCAGGCAGGACTGGAGGTCAGCTAAGAAGGTGCAAGTATTTCAGTATGTTGTGCTCAAGTGGGCTTTTGCGCTTCTCATCGGGATCGTTACTGGCCTCGTTGGCTTCTTCAATAATATTGCAGTTGAGAATATAGCTGGATTCAAGCTTCTTCTGACCACAGCTCTCATGTCTGACAAAAAATATTACAAGGCTTTTGCGGCATATGCTGCCATCAATGTAGCTCTAGCAGCAGCTGCAGCAGCTCTTTGTGCTTTTATTGCTCCTGCCGCAGCAGGTTCCGGCATTCCTGAGGTTAAAGCTTATCTAAATGGTGTCGATGCACATTCAATACTCGCTCCAAGCACTCTCTTTGTCAAGATTTTTGGTTCCATTCTTGGGGTGTCAGCCGGATTCGTGGTTGGTAAAGAAGGGCCCATGGTTCATACGGGTGCTTGCATAGCCTCTTTCCTTGGGCAGGGGGGCTCTCGCAAGTATCATTTAACTTGGTCATGGCTAAGATACTTCAAGAATGATTGCGACCGAAGAGACTTGATCACATGTGGTGCTGCTGCTGGTGTGGCTGCTGCATTTCGTGCCCCAGTTGGTGGTGTTCTGTTTGCTCTTGAGGAAGCAGCTTCGTGGTGGCGGAGTGCTCTGCTTTGGAGGACATTTTTCACAACAGCAGTTGTTGCAATAGTTTTGAGAGGCTTTATACAATATTGTACTACAGGAAATTGTGGGTTATTTGGGGAAGGAGGTCTGATCATGTATGATGTCAGTGCAGCCGGTGCAACATATAGTGGCCCCGATATACTAGCAGTGTTATTCCTAGGAACTGTTGGAGGTATTCTTGGAAGCATATATAACTATTTCGTGAACAAGGTCATCCGAACTTATAGCATCATTCATGAAAAGGGTGCTGCTCCCAAAATCTTACTCGTTATCACCATTGCCCTCTTGACATCATGTCTTTCTTATGGCTTGCCATGGTTTGCAGAGTGCCAACCATGCCCTACTGACGAGAGTGTGAGTTGTCCTACTGTTGGCAAATCTGGTAACTATAAAAGTTTCAATTGCCCCTCGGGCTATTATAATGACCTCGCCTCCCTCTTTCTAAACACCAATGATGATGCCATTCGTAATCTATTCAGCACCAGTACAAAAAAAGAATTTCACATTTCCTCACTTTTCATCTTCTTTGCCGCCATTTACTGCCTCGGAATTATCACTTATGGCATTGCTATTCCTTCTGGGCTCTTTATCCCTGTCATATTAGCTGGAGCTTGCTATGGACGTCTTGTTGGGAGGTTGTTTGAATCAATCTCTACTCTAGACACAGGTCTATTTGCTCTACTAGGAGCTGCCTCCTTCCTGGGTGGCACTATGAGAATGACAGTCTCACTCTGCATTATACTGCTTGAACTCACGAATGACTTACTGTTGCTTCCCCTTGTGATGTTGGTTCTCCTTATTTCAAAAACTGTGGCTGATAACTTCAACAAGGGAATTTACGACCAGATAGTCAAAATAAAGGGACTGCCTTATTTGGAGGCTCATGCAGAACCTTACATGAGGCAATTGGTCACAGCTGATGTTGCTACTGGACCATTGGTTACTTTTTCTGGGATTGAGAAGGTAGGGCGTATATTGGATGCTTTGAGGACTACGGGGCACAATGGATTTCCGGTGATTGATGAACCGCCTTTTTCGGATGCTCCAGAGTTGTGCGGTCTTGTTTTAAGGTCTCATTTGCTTATTTTGCTCAAGGGTAAGGTTTTTTCGAGGGAAAGGGTTGTTTGTGAAGAAGAGATCCTAGGGAGATTTGCCGCATTTAATTTCGCAAAGGCAGGGTCAGGAAAGGGAATCAAAGTGGAGGATCTTGATATTGAAGAGGAGGAGATGGATATGTATGTTGATCTACATCCTATTACAAATGCATCTCCGTACACAGTTGTTGAGACAATGTCACTTGCCAAAGCTGCTATTCTCTTTCGGCAACTCGGTCTCAGACACCTATGTTTGGTACCAAAGAGCCAAGGGAGACCTCCAATCGTCGGAATTCTAACGCGACATGACTTCATGCCGGAGCACATTTTGGGACTCTACCCGCATATAAAGGCTCATAAGTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005247 GO:0005253 GO:0005254 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006821 GO:0008150 GO:0008308 GO:0008509 GO:0009705 GO:0015075 GO:0015103 GO:0015108 GO:0015267 GO:0015318 GO:0015698 GO:0016020 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0098588 GO:0098656 GO:0098660 GO:0098661 GO:0098805 GO:1902476

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

779

Amino Acids

84.18

Weight (kDa)

6.91

Isoelectric Point (pI)

39.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	151 - 565	2.4e-76	Voltage gated chloride channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006543)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49890
fragaria_vesca	FvH4_1g25850 FvH4_1g25850
malus_domestica	MD00G1030600.v1.1
prunus_persica	Prupe.1G583800_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1
pyrus_communis	pycom08g21410 pycom520g01280
rosa_chinensis	RchiOBHm_Chr3g0484991
rosa_laevigata	RLG00000023179
rosa_multiflora	Rmu_sc0006040.1_g000024 Rmu_sc0009462.1_g000005
rosa_roxburghii	Rroxscaffold_6G00397280
rosa_rugosa	Rorug03G0214900.1 Rorug03G0215000
rosa_samantha	Rh3AG265300 Rh3CG298700 Rh3DG294600
rosa_wichuraiana	Rw0G006760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1296
AasI	GACNNNNNNGTC	1 cut(s)	1724
AatII	GACGTC	1 cut(s)	1501
Acc36I	ACCTGC	1 cut(s)	488
Acc65I	GGTACC	1 cut(s)	2227
AccB1I	GGYRCC	1 cut(s)	2227
AccB7I	CCANNNNNTGG	1 cut(s)	809
AccII	CGCG	1 cut(s)	2272
AciI	CCGC	8 cut(s)	422, 492, 850, 1407, 1904, 1932, 2031, 2312
AclWI	GGATC	3 cut(s)	314, 2008, 2088
AcsI	RAATTY	4 cut(s)	76, 1373, 1710, 2262
AcyI	GRCGYC	1 cut(s)	1498
AfaI	GTAC	4 cut(s)	928, 1363, 2150, 2229
AfiI	CCNNNNNNNGG	6 cut(s)	600, 679, 809, 1247, 2224, 2258
AflIII	ACRYGT	2 cut(s)	107, 761
AhdI	GACNNNNNGTC	2 cut(s)	238, 1170
AjnI	CCWGG	1 cut(s)	1575
Alw21I	GWGCWC	3 cut(s)	279, 859, 2295
Alw26I	GTCTC	6 cut(s)	744, 1606, 1953, 2156, 2213, 2239
AlwI	GGATC	3 cut(s)	314, 2008, 2088
AlwNI	CAGNNNCTG	1 cut(s)	458
Ama87I	CYCGRG	1 cut(s)	1285
AoxI	GGCC	3 cut(s)	316, 635, 1006
ApaI	GGGCCC	1 cut(s)	639
ApoI	RAATTY	4 cut(s)	76, 1373, 1710, 2262
ArsI	GACNNNNNNTTYG	2 cut(s)	2039, 2071
Asp718I	GGTACC	1 cut(s)	2227
AspA2I	CCTAGG	2 cut(s)	1033, 2017
AspLEI	GCGC	1 cut(s)	295
AspS9I	GGNCC	4 cut(s)	635, 636, 1007, 1808
AsuHPI	GGTGA	3 cut(s)	61, 1141, 1903
AvaI	CYCGRG	1 cut(s)	1285
AvaII	GGWCC	1 cut(s)	1808
AvrII	CCTAGG	2 cut(s)	1033, 2017
AxyI	CCTNAGG	1 cut(s)	513
BaeGI	GKGCMC	3 cut(s)	639, 802, 1878
BanI	GGYRCC	1 cut(s)	2227
BanII	GRGCYC	3 cut(s)	639, 689, 1461
Bbv12I	GWGCWC	3 cut(s)	279, 859, 2295
BccI	CCATC	5 cut(s)	53, 86, 191, 443, 2098
BciT130I	CCWGG	1 cut(s)	1577
BclI	TGATCA	2 cut(s)	756, 966
BcoDI	GTCTC	6 cut(s)	744, 1606, 1953, 2156, 2213, 2239
BfaI	CTAG	6 cut(s)	449, 1019, 1034, 1535, 1559, 2018
BfmI	CTRYAG	2 cut(s)	459, 930
BfuAI	ACCTGC	1 cut(s)	488
BglI	GCCNNNNNGGC	2 cut(s)	324, 1287
BlnI	CCTAGG	2 cut(s)	1033, 2017
Bme1390I	CCNGG	1 cut(s)	1577
Bme18I	GGWCC	1 cut(s)	1808
BmeRI	GACNNNNNGTC	2 cut(s)	238, 1170
BmeT110I	CYCGRG	1 cut(s)	1285
BmgT120I	GGNCC	4 cut(s)	635, 636, 1007, 1808
BmiI	GGNNCC	6 cut(s)	169, 502, 592, 637, 1009, 2229
BmrFI	CCNGG	1 cut(s)	1577
BmrI	ACTGGG	1 cut(s)	797
BmsI	GCATC	6 cut(s)	532, 1113, 1324, 1846, 1906, 2150
BmuI	ACTGGG	1 cut(s)	797
BoxI	GACNNNNGTC	1 cut(s)	1542
BpmI	CTGGAG	3 cut(s)	256, 1503, 1905
BpuEI	CTTGAG	3 cut(s)	263, 848, 1952
BsaBI	GATNNNNATC	1 cut(s)	2124
BsaHI	GRCGYC	1 cut(s)	1498
BsaI	GGTCTC	3 cut(s)	1953, 2213, 2239
BsaJI	CCNNGG	9 cut(s)	639, 673, 1033, 1190, 1284, 1420, 1576, 2017, 2239
BsaWI	WCCGGW	1 cut(s)	1888
BsaXI	ACNNNNNCTCC	4 cut(s)	94, 124, 1292, 1322
Bsc4I	CCNNNNNNNGG	6 cut(s)	600, 679, 809, 1247, 2224, 2258
Bse118I	RCCGGY	1 cut(s)	989
Bse1I	ACTGG	5 cut(s)	239, 319, 803, 1359, 1810
Bse21I	CCTNAGG	1 cut(s)	513
Bse3DI	GCAATG	2 cut(s)	1152, 1440
Bse8I	GATNNNNATC	1 cut(s)	2124
BseBI	CCWGG	1 cut(s)	1577
BseDI	CCNNGG	9 cut(s)	639, 673, 1033, 1190, 1284, 1420, 1576, 2017, 2239
BseGI	GGATG	5 cut(s)	97, 1089, 1861, 1921, 2125
BseJI	GATNNNNATC	1 cut(s)	2124
BseLI	CCNNNNNNNGG	6 cut(s)	600, 679, 809, 1247, 2224, 2258
BseMI	GCAATG	2 cut(s)	1152, 1440
BseMII	CTCAG	3 cut(s)	164, 504, 2224
BseNI	ACTGG	5 cut(s)	239, 319, 803, 1359, 1810
BseRI	GAGGAG	3 cut(s)	115, 2111, 2114
BseSI	GKGCMC	3 cut(s)	639, 802, 1878
BsgI	GTGCAG	1 cut(s)	1005
Bsh1236I	CGCG	1 cut(s)	2272
Bsh1285I	CGRYCG	1 cut(s)	745
BshFI	GGCC	3 cut(s)	318, 637, 1008
BshNI	GGYRCC	1 cut(s)	2227
BsiEI	CGRYCG	1 cut(s)	745
BsiHKAI	GWGCWC	3 cut(s)	279, 859, 2295
BsiHKCI	CYCGRG	1 cut(s)	1285
BsiSI	CCGG	5 cut(s)	504, 612, 990, 1889, 2288
BslFI	GGGAC	2 cut(s)	1752, 2316
BslI	CCNNNNNNNGG	6 cut(s)	600, 679, 809, 1247, 2224, 2258
BsmAI	GTCTC	6 cut(s)	744, 1606, 1953, 2156, 2213, 2239
BsmFI	GGGAC	2 cut(s)	1752, 2316
BsmI	GAATGC	1 cut(s)	507
BsnI	GGCC	3 cut(s)	318, 637, 1008
Bso31I	GGTCTC	3 cut(s)	1953, 2213, 2239
BsoBI	CYCGRG	1 cut(s)	1285
Bsp120I	GGGCCC	1 cut(s)	635
Bsp1286I	GDGCHC	8 cut(s)	279, 639, 689, 802, 859, 1461, 1878, 2295
Bsp143I	GATC	6 cut(s)	306, 756, 966, 2013, 2080, 2119
Bsp19I	CCATGG	2 cut(s)	639, 1190
BspACI	CCGC	8 cut(s)	422, 492, 850, 1407, 1904, 1932, 2031, 2312
BspANI	GGCC	3 cut(s)	318, 637, 1008
BspCNI	CTCAG	3 cut(s)	163, 505, 2223
BspFNI	CGCG	1 cut(s)	2272
BspHI	TCATGA	1 cut(s)	1111
BspLI	GGNNCC	6 cut(s)	169, 502, 592, 637, 1009, 2229
BspMAI	CTGCAG	1 cut(s)	463
BspMI	ACCTGC	1 cut(s)	488
BspPI	GGATC	3 cut(s)	314, 2008, 2088
BspT107I	GGYRCC	1 cut(s)	2227
BspTNI	GGTCTC	3 cut(s)	1953, 2213, 2239
BsrDI	GCAATG	2 cut(s)	1152, 1440
BsrFI	RCCGGY	1 cut(s)	989
BsrI	ACTGG	5 cut(s)	239, 319, 803, 1359, 1810
BssAI	RCCGGY	1 cut(s)	989
BssECI	CCNNGG	9 cut(s)	639, 673, 1033, 1190, 1284, 1420, 1576, 2017, 2239
BssMI	GATC	6 cut(s)	306, 756, 966, 2013, 2080, 2119
BssNI	GRCGYC	1 cut(s)	1498
BssT1I	CCWWGG	6 cut(s)	639, 673, 1033, 1190, 2017, 2239
Bst2UI	CCWGG	1 cut(s)	1577
Bst4CI	ACNGT	6 cut(s)	1042, 1246, 1600, 1644, 1687, 2155
Bst6I	CTCTTC	3 cut(s)	742, 2004, 2088
BstACI	GRCGYC	1 cut(s)	1498
BstAPI	GCANNNNNTGC	5 cut(s)	431, 458, 797, 893, 992
BstC8I	GCNNGC	3 cut(s)	658, 1187, 1489
BstDEI	CTNAG	6 cut(s)	125, 150, 246, 513, 719, 2210
BstDSI	CCRYGG	2 cut(s)	639, 1190
BstENI	CCTNNNNNAGG	1 cut(s)	677
BstF5I	GGATG	5 cut(s)	97, 1089, 1861, 1921, 2125
BstFNI	CGCG	1 cut(s)	2272
BstHHI	GCGC	1 cut(s)	295
BstKTI	GATC	6 cut(s)	309, 759, 969, 2016, 2083, 2122
BstMAI	GTCTC	6 cut(s)	744, 1606, 1953, 2156, 2213, 2239
BstMBI	GATC	6 cut(s)	306, 756, 966, 2013, 2080, 2119
BstMCI	CGRYCG	1 cut(s)	745
BstNI	CCWGG	1 cut(s)	1577
BstNSI	RCATGY	2 cut(s)	111, 765
BstPAI	GACNNNNGTC	1 cut(s)	1542
BstSCI	CCNGG	1 cut(s)	1575
BstSFI	CTRYAG	2 cut(s)	459, 930
BstSLI	GKGCMC	3 cut(s)	639, 802, 1878
BstUI	CGCG	1 cut(s)	2272
BstX2I	RGATCY	2 cut(s)	2013, 2080
BstYI	RGATCY	2 cut(s)	2013, 2080
Bsu36I	CCTNAGG	1 cut(s)	513
BsuRI	GGCC	3 cut(s)	318, 637, 1008
BtgI	CCRYGG	2 cut(s)	639, 1190
BtsCI	GGATG	5 cut(s)	97, 1089, 1861, 1921, 2125
BtsI	GCAGTG	1 cut(s)	1029
BtsIMutI	CAGTG	2 cut(s)	988, 1029
BveI	ACCTGC	1 cut(s)	488
Cac8I	GCNNGC	3 cut(s)	658, 1187, 1489
CaiI	CAGNNNCTG	1 cut(s)	458
CciI	TCATGA	1 cut(s)	1111
CfoI	GCGC	1 cut(s)	295
Cfr10I	RCCGGY	1 cut(s)	989
Cfr13I	GGNCC	4 cut(s)	635, 636, 1007, 1808
Csp6I	GTAC	4 cut(s)	927, 1362, 2149, 2228
CspCI	CAANNNNNGTGG	2 cut(s)	1668, 1703
CviQI	GTAC	4 cut(s)	927, 1362, 2149, 2228
DdeI	CTNAG	6 cut(s)	125, 150, 246, 513, 719, 2210
DpnI	GATC	6 cut(s)	308, 758, 968, 2015, 2082, 2121
DpnII	GATC	6 cut(s)	306, 756, 966, 2013, 2080, 2119
DrdI	GACNNNNNNGTC	1 cut(s)	1724
DriI	GACNNNNNGTC	2 cut(s)	238, 1170
DseDI	GACNNNNNNGTC	1 cut(s)	1724
Eam1104I	CTCTTC	3 cut(s)	742, 2004, 2088
Eam1105I	GACNNNNNGTC	2 cut(s)	238, 1170
EarI	CTCTTC	3 cut(s)	742, 2004, 2088
EciI	GGCGGA	1 cut(s)	865
Eco130I	CCWWGG	6 cut(s)	639, 673, 1033, 1190, 2017, 2239
Eco24I	GRGCYC	3 cut(s)	639, 689, 1461
Eco31I	GGTCTC	3 cut(s)	1953, 2213, 2239
Eco47I	GGWCC	1 cut(s)	1808
Eco81I	CCTNAGG	1 cut(s)	513
Eco88I	CYCGRG	1 cut(s)	1285
EcoNI	CCTNNNNNAGG	1 cut(s)	677
EcoO109I	RGGNCCY	1 cut(s)	635
EcoRI	GAATTC	1 cut(s)	2262
EcoRII	CCWGG	1 cut(s)	1575
EcoT14I	CCWWGG	6 cut(s)	639, 673, 1033, 1190, 2017, 2239
EcoT22I	ATGCAT	1 cut(s)	2143
EcoT38I	GRGCYC	3 cut(s)	639, 689, 1461
ErhI	CCWWGG	6 cut(s)	639, 673, 1033, 1190, 2017, 2239
FaqI	GGGAC	2 cut(s)	1752, 2316
FauI	CCCGC	1 cut(s)	2319
FauNDI	CATATG	1 cut(s)	427
FbaI	TGATCA	2 cut(s)	756, 966
FokI	GGATG	5 cut(s)	104, 1076, 1868, 1928, 2112
FriOI	GRGCYC	3 cut(s)	639, 689, 1461
FspBI	CTAG	6 cut(s)	449, 1019, 1034, 1535, 1559, 2018
GlaI	GCGC	1 cut(s)	294
GsuI	CTGGAG	3 cut(s)	256, 1503, 1905
HaeIII	GGCC	3 cut(s)	318, 637, 1008
HapII	CCGG	5 cut(s)	504, 612, 990, 1889, 2288
HhaI	GCGC	1 cut(s)	295
Hin1I	GRCGYC	1 cut(s)	1498
Hin6I	GCGC	1 cut(s)	293
HinP1I	GCGC	1 cut(s)	293
HindIII	AAGCTT	2 cut(s)	368, 522
HinfI	GANTC	5 cut(s)	363, 615, 1520, 2067, 2304
HpaII	CCGG	5 cut(s)	504, 612, 990, 1889, 2288
HphI	GGTGA	3 cut(s)	61, 1141, 1903
Hpy166II	GTNNAC	2 cut(s)	1081, 2151
Hpy188I	TCNGA	9 cut(s)	153, 378, 397, 966, 1094, 1424, 1915, 2213, 2261
Hpy8I	GTNNAC	2 cut(s)	1081, 2151
Hpy99I	CGWCG	1 cut(s)	2261
HpyAV	CCTTC	7 cut(s)	90, 244, 626, 950, 1461, 1582, 1831
HpyCH4III	ACNGT	6 cut(s)	1042, 1246, 1600, 1644, 1687, 2155
HpyCH4IV	ACGT	1 cut(s)	1498
HpyF3I	CTNAG	6 cut(s)	125, 150, 246, 513, 719, 2210
HpySE526I	ACGT	1 cut(s)	1498
Hsp92I	GRCGYC	1 cut(s)	1498
HspAI	GCGC	1 cut(s)	293
KpnI	GGTACC	1 cut(s)	2231
Ksp22I	TGATCA	2 cut(s)	756, 966
Kzo9I	GATC	6 cut(s)	306, 756, 966, 2013, 2080, 2119
LmnI	GCTCC	8 cut(s)	185, 490, 565, 1132, 1484, 1562, 1924, 2290
LweI	GCATC	6 cut(s)	532, 1113, 1324, 1846, 1906, 2150
MaeI	CTAG	6 cut(s)	449, 1019, 1034, 1535, 1559, 2018
MaeII	ACGT	1 cut(s)	1498
MaeIII	GTNAC	6 cut(s)	53, 310, 1259, 1786, 1816, 2169
MalI	GATC	6 cut(s)	308, 758, 968, 2015, 2082, 2121
MboI	GATC	6 cut(s)	306, 756, 966, 2013, 2080, 2119
MboII	GAAGA	9 cut(s)	23, 148, 322, 365, 759, 1390, 2018, 2021, 2105
MfeI	CAATTG	2 cut(s)	1276, 1781
MflI	RGATCY	2 cut(s)	2013, 2080
MhlI	GDGCHC	8 cut(s)	279, 639, 689, 802, 859, 1461, 1878, 2295
MluCI	AATT	9 cut(s)	76, 937, 1276, 1373, 1425, 1710, 1781, 2038, 2262
MlyI	GAGTC	1 cut(s)	2298
MmeI	TCCRAC	3 cut(s)	69, 1024, 2239
Mph1103I	ATGCAT	1 cut(s)	2143
MseI	TTAA	4 cut(s)	519, 704, 1943, 2037
MslI	CAYNNNNRTG	1 cut(s)	651
MspA1I	CMGCKG	2 cut(s)	458, 1793
MspI	CCGG	5 cut(s)	504, 612, 990, 1889, 2288
MspR9I	CCNGG	1 cut(s)	1577
MunI	CAATTG	2 cut(s)	1276, 1781
Mva1269I	GAATGC	1 cut(s)	507
MvaI	CCWGG	1 cut(s)	1577
MvnI	CGCG	1 cut(s)	2272
NcoI	CCATGG	2 cut(s)	639, 1190
NdeI	CATATG	1 cut(s)	427
NdeII	GATC	6 cut(s)	306, 756, 966, 2013, 2080, 2119
NlaIV	GGNNCC	6 cut(s)	169, 502, 592, 637, 1009, 2229
NmuCI	GTSAC	3 cut(s)	53, 1786, 2169
NsiI	ATGCAT	1 cut(s)	2143
NspI	RCATGY	2 cut(s)	111, 765
PagI	TCATGA	1 cut(s)	1111
PciI	ACATGT	2 cut(s)	107, 761
PcsI	WCGNNNNNNNCGW	1 cut(s)	63
PctI	GAATGC	1 cut(s)	507
PfeI	GAWTC	4 cut(s)	363, 615, 1520, 2067
PflFI	GACNNNGTC	1 cut(s)	2167
PflMI	CCANNNNNTGG	1 cut(s)	809
PleI	GAGTC	1 cut(s)	2298
PpsI	GAGTC	1 cut(s)	2298
PscI	ACATGT	2 cut(s)	107, 761
PshAI	GACNNNNGTC	1 cut(s)	1542
PsiI	TTATAA	1 cut(s)	1296
Psp6I	CCWGG	1 cut(s)	1575
PspGI	CCWGG	1 cut(s)	1575
PspN4I	GGNNCC	6 cut(s)	169, 502, 592, 637, 1009, 2229
PspOMI	GGGCCC	1 cut(s)	635
PspPI	GGNCC	4 cut(s)	635, 636, 1007, 1808
PstI	CTGCAG	1 cut(s)	463
PstNI	CAGNNNCTG	1 cut(s)	458
PsuI	RGATCY	2 cut(s)	2013, 2080
PsyI	GACNNNGTC	1 cut(s)	2167
PvuII	CAGCTG	2 cut(s)	458, 1793
RsaI	GTAC	4 cut(s)	928, 1363, 2150, 2229
RsaNI	GTAC	4 cut(s)	927, 1362, 2149, 2228
RseI	CAYNNNNRTG	1 cut(s)	651
SaqAI	TTAA	4 cut(s)	519, 704, 1943, 2037
Sau3AI	GATC	6 cut(s)	306, 756, 966, 2013, 2080, 2119
Sau96I	GGNCC	4 cut(s)	635, 636, 1007, 1808
SchI	GAGTC	1 cut(s)	2298
ScrFI	CCNGG	1 cut(s)	1577
SduI	GDGCHC	8 cut(s)	279, 639, 689, 802, 859, 1461, 1878, 2295
SfaNI	GCATC	6 cut(s)	532, 1113, 1324, 1846, 1906, 2150
SfcI	CTRYAG	2 cut(s)	459, 930
SinI	GGWCC	1 cut(s)	1808
SmiMI	CAYNNNNRTG	1 cut(s)	651
SmlI	CTYRAG	3 cut(s)	278, 827, 1967
SmoI	CTYRAG	3 cut(s)	278, 827, 1967
Sse9I	AATT	9 cut(s)	76, 937, 1276, 1373, 1425, 1710, 1781, 2038, 2262
SsiI	CCGC	8 cut(s)	422, 492, 850, 1407, 1904, 1932, 2031, 2312
SspI	AATATT	4 cut(s)	222, 340, 407, 923
SspMI	CTAG	6 cut(s)	449, 1019, 1034, 1535, 1559, 2018
StyD4I	CCNGG	1 cut(s)	1575
StyI	CCWWGG	6 cut(s)	639, 673, 1033, 1190, 2017, 2239
TaaI	ACNGT	6 cut(s)	1042, 1246, 1600, 1644, 1687, 2155
TaiI	ACGT	1 cut(s)	1501
TaqI	TCGA	3 cut(s)	186, 540, 1985
TaqII	GACCGA	2 cut(s)	759, 2195
TasI	AATT	9 cut(s)	76, 937, 1276, 1373, 1425, 1710, 1781, 2038, 2262
TatI	WGTACW	2 cut(s)	926, 1361
TauI	GCSGC	4 cut(s)	425, 494, 1409, 2033
TfiI	GAWTC	4 cut(s)	363, 615, 1520, 2067
Tru1I	TTAA	4 cut(s)	519, 704, 1943, 2037
Tru9I	TTAA	4 cut(s)	519, 704, 1943, 2037
TscAI	CASTG	2 cut(s)	988, 1029
TseFI	GTSAC	3 cut(s)	53, 1786, 2169
Tsp45I	GTSAC	3 cut(s)	53, 1786, 2169
TspDTI	ATGAA	9 cut(s)	20, 24, 231, 635, 1100, 1128, 1384, 1914, 2272
TspGWI	ACGGA	1 cut(s)	2136
TspRI	CASTG	2 cut(s)	988, 1029
Tth111I	GACNNNGTC	1 cut(s)	2167
Van91I	CCANNNNNTGG	1 cut(s)	809
VpaK11BI	GGWCC	1 cut(s)	1808
XagI	CCTNNNNNAGG	1 cut(s)	677
XapI	RAATTY	4 cut(s)	76, 1373, 1710, 2262
XbaI	TCTAGA	1 cut(s)	1534
XceI	RCATGY	2 cut(s)	111, 765
XmaJI	CCTAGG	2 cut(s)	1033, 2017
XspI	CTAG	6 cut(s)	449, 1019, 1034, 1535, 1559, 2018
ZraI	GACGTC	1 cut(s)	1499
Zsp2I	ATGCAT	1 cut(s)	2143

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.