Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0006543

Physical Location & Seq

Genomic Coordinates Reverse (-)

18942060 .. 18949214

Gene Structure 7155 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00397280.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2127 bp

ATGGATCCAGAAAGGAATAGCAGCGGTCAATTCCTTGTTGGTGTTGGTAGTGACCTTGAGAATTTTGATTTGTTGGAGAAGGAGACTGAGAATGAGAACGACAGGACATACAGCTACAGAGAACCGCTTCTGAGGAAAAGGATCAACACAACATCTCAGATTGCCATCGTTGGTGCCAATGTCTGCTCCATTGAGAGCCTTGACTATGAATATGTTGCTGACTTTGGCACAATTTCATCTTTGATGTTTTTAGCTTTGTGCAAACAATATTTGAAGTTGTGTTTCTGTAAGGTCTTCATTTCCCACCTGATATTGAGTAACATTGATTTTGGGCTGATATTTGATGTGTATCTCTTTGGGTACTTGAGAACTTTGTGGGAGGACATTCTTCTCAGAATATATTACAAGGCTTTTGCAGTACTTGCTTCCATGAACGTAGGTTTAGCAACAGTTGCTGCGGCTCTTTGTGCTTTCATTGCTCCTGCAGCAGCAGGTTCTGGTATTCCGGAGGTCAAAGCTTATTTAAATGGAATAGATGCATATTCAATACTGGCTCCAAGCACCCTTTTTGTCAAGATTTTTGGTTCCATTCTTTGTGTTTCTGCCGGATTTGTGGTTGGTAAAGAAGGCCCCATGGTGGCGGAGTGCTCTTCTTTGGAGAACCTTTTCACGACAGCAGTAGTTGCAATTGTACTGAGAGGTTTCGTACAGTACTGTGCTACAGGAAACTGTGGCCTATTTGGGGAAGGCGGTCTGATTATGTACGACGTCAGTGCAGCTGACGCAACATATAGTGCCCCCGATATACTAGCAGTATTACTCCTCGGTGTTTTTGGAGGAATTTTTGGAAGTATATATAACTATTGTGTTGACAAGGTCATCCGAACTTATAGCATCATTCATGAAAAAGGTGCTGCTCCCAAGATCTTACTTGTTATCACCATTTCCCTATTGACGTCATCCATTGCTTTTGGCTTGCCATGGTTTGCAAGGTGCATCGCATGCCCTTCTGATCTGACTGTCACTTGTCCTACTGTTGGGAAAACTGGCAACTACATAAGCTTCAACTGCCCATCAGGCTATTACAATGACATGGCCTCCCTCTTTCTGAACACTAACGACGATGCTATTCGTAATCTATTTAGCACCGATACAACAAAAGAATTCCACATATACTCTCTTTTCATCTTCTTTGCTGCTGTTTACATCCTTGGAATTATTACATATGGCATTGCTATTCCTTCTGGACTTTTCATCCCTGTCATACTAGCCGGAGCTTGCTATGGTCGTCTTGTTGGGAGGTTGTTTGAATCAATATCTACTCTCGACACAGGCCTCTTTGCCCTACTTGGAGCTGCCTCGTTCCTTGGTGGCACCATGCGGATGACAGTTTCCCTATGCGTCATATTGCTTGAACTTACCAATGACTTACTGTTGCTTCCCCTGGTGATGTTGGTCCTGCTTATTTCAAAAACTGTGGCTGATAACTTTAACAAAGGAATTTATGACCAGATAGTTAAGATAAAGGGATTGCCTTATTTGGAGGCTCATGCAGAACCTTACATGAAGCATTTGGTCGCAAGTGATGTTGTTTCAGGTCCATTGGTTACCTTTTCTGGTGTCGAGAAGGTAGGCTGTATACTGCATGCTTTGAGGACCACAGGGCATAATGGATTCCCAGTGATTGATGAACCACCTTTTTCGGACACTCCCGAGTTGTGTGGTCTTGTTTTAAGGTCCCATTTGCTTACTTTGCTCAACGGTAAGATCTTTTCAAAGGAAAGGATTGTTTGTGGAGAAGAGATCTTACGTAGATTTGATGCATTTGATTTTGCCAAGGCGGGATCAGGAAAGGGACTCAAAGTGGAAGATCTTAATATTGAAGAGGAAGAGATGGAAATGTATGTTGACCTCCATCCTATTACAAATGCATCCCCATACACAGTTGTTGAGACAATGTCACTAGCCAAAGCCGCTATTCTGTTTCGACAACTTGGTCTCAGGCACTTATGTTTGGTACCAAAGAGCCAAGGGAGATCTCCAATCGTCGGAATTCTGACACGGCATGACTTTATGCCAGAGCACATTCTGGGACTCTTCCCTAATATCAAGCCTCACAACAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005247 GO:0005253 GO:0005254 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006821 GO:0008150 GO:0008308 GO:0008509 GO:0009705 GO:0015075 GO:0015103 GO:0015108 GO:0015267 GO:0015318 GO:0015698 GO:0016020 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0098588 GO:0098656 GO:0098660 GO:0098661 GO:0098805 GO:1902476

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

708

Amino Acids

77.22

Weight (kDa)

5.41

Isoelectric Point (pI)

33.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	149 - 214	3e-12	Voltage gated chloride channel
Voltage_CLC	PF00654	223 - 495	7.2e-33	Voltage gated chloride channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006543)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49890
fragaria_vesca	FvH4_1g25850 FvH4_1g25850
malus_domestica	MD00G1030600.v1.1
prunus_persica	Prupe.1G583800_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1 Prupe.4G288000_v2.0.a1
pyrus_communis	pycom08g21410 pycom520g01280
rosa_chinensis	RchiOBHm_Chr3g0484991
rosa_laevigata	RLG00000023179
rosa_multiflora	Rmu_sc0006040.1_g000024 Rmu_sc0009462.1_g000005
rosa_roxburghii	Rroxscaffold_6G00397280
rosa_rugosa	Rorug03G0214900.1 Rorug03G0215000
rosa_samantha	Rh3AG265300 Rh3CG298700 Rh3DG294600
rosa_wichuraiana	Rw0G006760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1995
AatII	GACGTC	2 cut(s)	771, 959
Acc36I	ACCTGC	1 cut(s)	482
Acc65I	GGTACC	1 cut(s)	2017
AccB1I	GGYRCC	3 cut(s)	173, 1373, 2017
AccI	GTMKAC	1 cut(s)	1639
AccIII	TCCGGA	1 cut(s)	505
AciI	CCGC	8 cut(s)	24, 125, 458, 641, 750, 1381, 1841, 1974
AclWI	GGATC	3 cut(s)	12, 149, 1852
AcsI	RAATTY	5 cut(s)	61, 840, 1163, 1500, 2052
AcyI	GRCGYC	2 cut(s)	768, 956
AfaI	GTAC	7 cut(s)	362, 420, 693, 708, 713, 764, 2019
AfiI	CCNNNNNNNGG	4 cut(s)	637, 742, 1037, 2048
AgsI	TTSAA	8 cut(s)	274, 546, 1066, 1310, 1415, 1470, 1776, 1883
AjnI	CCWGG	1 cut(s)	1443
AluBI	AGCT	7 cut(s)	114, 254, 518, 779, 1062, 1277, 1355
AluI	AGCT	7 cut(s)	114, 254, 518, 779, 1062, 1277, 1355
Alw21I	GWGCWC	2 cut(s)	650, 2085
Alw26I	GTCTC	3 cut(s)	77, 1946, 2003
AlwI	GGATC	3 cut(s)	12, 149, 1852
AlwNI	CAGNNNCTG	2 cut(s)	455, 497
Ama87I	CYCGRG	1 cut(s)	1712
Aor13HI	TCCGGA	1 cut(s)	505
AoxI	GGCC	4 cut(s)	628, 733, 1095, 1333
ApeKI	GCWGC	8 cut(s)	21, 455, 485, 488, 776, 914, 1196, 1355
ApoI	RAATTY	5 cut(s)	61, 840, 1163, 1500, 2052
Asp700I	GAANNNNTTC	2 cut(s)	126, 665
Asp718I	GGTACC	1 cut(s)	2017
AspS9I	GGNCC	5 cut(s)	629, 1456, 1598, 1656, 1737
AsuHPI	GGTGA	2 cut(s)	931, 1459
AvaI	CYCGRG	1 cut(s)	1712
AvaII	GGWCC	4 cut(s)	1456, 1598, 1656, 1737
BaeGI	GKGCMC	1 cut(s)	799
BamHI	GGATCC	1 cut(s)	4
BanI	GGYRCC	3 cut(s)	173, 1373, 2017
BarI	GAAGNNNNNNTAC	2 cut(s)	1791, 1823
BbsI	GAAGAC	1 cut(s)	286
Bbv12I	GWGCWC	2 cut(s)	650, 2085
BbvI	GCAGC	8 cut(s)	33, 442, 497, 500, 788, 901, 1183, 1342
BccI	CCATC	4 cut(s)	173, 1081, 1888, 1923
BceAI	ACGGC	1 cut(s)	2078
BcgI	CGANNNNNNTGC	2 cut(s)	755, 789
BciT130I	CCWGG	1 cut(s)	1445
BcoDI	GTCTC	3 cut(s)	77, 1946, 2003
BfaI	CTAG	4 cut(s)	809, 1268, 1964, 2125
BfmI	CTRYAG	3 cut(s)	115, 483, 720
BfuAI	ACCTGC	1 cut(s)	482
BglI	GCCNNNNNGGC	1 cut(s)	1077
BglII	AGATCT	5 cut(s)	924, 1767, 1803, 1870, 2036
BmcAI	AGTACT	2 cut(s)	420, 713
Bme1390I	CCNGG	1 cut(s)	1445
Bme18I	GGWCC	4 cut(s)	1456, 1598, 1656, 1737
BmeT110I	CYCGRG	1 cut(s)	1712
BmgT120I	GGNCC	5 cut(s)	629, 1456, 1598, 1656, 1737
BmiI	GGNNCC	8 cut(s)	6, 175, 555, 586, 631, 1375, 1739, 2019
BmrFI	CCNGG	1 cut(s)	1445
BmrI	ACTGGG	1 cut(s)	1673
BmsI	GCATC	6 cut(s)	526, 903, 1005, 1114, 1810, 1940
BmuI	ACTGGG	1 cut(s)	1673
BpiI	GAAGAC	1 cut(s)	286
BpuEI	CTTGAG	2 cut(s)	77, 385
BsaAI	YACGTR	1 cut(s)	1811
BsaBI	GATNNNNATC	2 cut(s)	164, 348
BsaHI	GRCGYC	2 cut(s)	768, 956
BsaI	GGTCTC	1 cut(s)	2003
BsaJI	CCNNGG	8 cut(s)	633, 823, 980, 1210, 1366, 1443, 1836, 2029
BsaWI	WCCGGW	1 cut(s)	505
BsaXI	ACNNNNNCTCC	2 cut(s)	1082, 1112
Bsc4I	CCNNNNNNNGG	4 cut(s)	637, 742, 1037, 2048
Bse1I	ACTGG	3 cut(s)	555, 1051, 1679
Bse3DI	GCAATG	3 cut(s)	474, 963, 1230
Bse8I	GATNNNNATC	2 cut(s)	164, 348
BseAI	TCCGGA	1 cut(s)	505
BseBI	CCWGG	1 cut(s)	1445
BseDI	CCNNGG	8 cut(s)	633, 823, 980, 1210, 1366, 1443, 1836, 2029
BseGI	GGATG	7 cut(s)	879, 959, 1206, 1254, 1389, 1915, 1931
BseJI	GATNNNNATC	2 cut(s)	164, 348
BseLI	CCNNNNNNNGG	4 cut(s)	637, 742, 1037, 2048
BseMI	GCAATG	3 cut(s)	474, 963, 1230
BseMII	CTCAG	6 cut(s)	78, 122, 170, 406, 686, 2014
BseNI	ACTGG	3 cut(s)	555, 1051, 1679
BseRI	GAGGAG	1 cut(s)	812
BseSI	GKGCMC	1 cut(s)	799
BseXI	GCAGC	8 cut(s)	33, 442, 497, 500, 788, 901, 1183, 1342
BsgI	GTGCAG	1 cut(s)	795
BshFI	GGCC	4 cut(s)	630, 735, 1097, 1335
BshNI	GGYRCC	3 cut(s)	173, 1373, 2017
BsiHKAI	GWGCWC	2 cut(s)	650, 2085
BsiHKCI	CYCGRG	1 cut(s)	1712
BsiSI	CCGG	3 cut(s)	506, 606, 1272
BslFI	GGGAC	3 cut(s)	1723, 1869, 2106
BslI	CCNNNNNNNGG	4 cut(s)	637, 742, 1037, 2048
BsmAI	GTCTC	3 cut(s)	77, 1946, 2003
BsmFI	GGGAC	3 cut(s)	1723, 1869, 2106
BsnI	GGCC	4 cut(s)	630, 735, 1097, 1335
Bso31I	GGTCTC	1 cut(s)	2003
BsoBI	CYCGRG	1 cut(s)	1712
Bsp1286I	GDGCHC	3 cut(s)	650, 799, 2085
Bsp13I	TCCGGA	1 cut(s)	505
Bsp143I	GATC	9 cut(s)	4, 141, 924, 1012, 1767, 1803, 1844, 1870, 2036
Bsp19I	CCATGG	2 cut(s)	633, 980
BspACI	CCGC	8 cut(s)	24, 125, 458, 641, 750, 1381, 1841, 1974
BspANI	GGCC	4 cut(s)	630, 735, 1097, 1335
BspCNI	CTCAG	6 cut(s)	79, 123, 169, 405, 687, 2013
BspEI	TCCGGA	1 cut(s)	505
BspHI	TCATGA	1 cut(s)	901
BspLI	GGNNCC	8 cut(s)	6, 175, 555, 586, 631, 1375, 1739, 2019
BspMAI	CTGCAG	1 cut(s)	487
BspMI	ACCTGC	1 cut(s)	482
BspPI	GGATC	3 cut(s)	12, 149, 1852
BspQI	GCTCTTC	1 cut(s)	655
BspT107I	GGYRCC	3 cut(s)	173, 1373, 2017
BspTNI	GGTCTC	1 cut(s)	2003
BsrDI	GCAATG	3 cut(s)	474, 963, 1230
BsrI	ACTGG	3 cut(s)	555, 1051, 1679
BssECI	CCNNGG	8 cut(s)	633, 823, 980, 1210, 1366, 1443, 1836, 2029
BssMI	GATC	9 cut(s)	4, 141, 924, 1012, 1767, 1803, 1844, 1870, 2036
BssNAI	GTATAC	1 cut(s)	1640
BssNI	GRCGYC	2 cut(s)	768, 956
BssT1I	CCWWGG	6 cut(s)	633, 980, 1210, 1366, 1836, 2029
Bst1107I	GTATAC	1 cut(s)	1640
Bst2UI	CCWGG	1 cut(s)	1445
Bst6I	CTCTTC	5 cut(s)	655, 1794, 1878, 1884, 2102
BstACI	GRCGYC	2 cut(s)	768, 956
BstAPI	GCANNNNNTGC	4 cut(s)	422, 452, 683, 1002
BstBAI	YACGTR	1 cut(s)	1811
BstC8I	GCNNGC	4 cut(s)	977, 1003, 1279, 1647
BstDEI	CTNAG	6 cut(s)	87, 131, 156, 392, 695, 2000
BstDSI	CCRYGG	2 cut(s)	633, 980
BstEII	GGTNACC	1 cut(s)	1606
BstF5I	GGATG	7 cut(s)	879, 959, 1206, 1254, 1389, 1915, 1931
BstKTI	GATC	9 cut(s)	7, 144, 927, 1015, 1770, 1806, 1847, 1873, 2039
BstMAI	GTCTC	3 cut(s)	77, 1946, 2003
BstMBI	GATC	9 cut(s)	4, 141, 924, 1012, 1767, 1803, 1844, 1870, 2036
BstNI	CCWGG	1 cut(s)	1445
BstNSI	RCATGY	2 cut(s)	1005, 1649
BstPI	GGTNACC	1 cut(s)	1606
BstSCI	CCNGG	1 cut(s)	1443
BstSFI	CTRYAG	3 cut(s)	115, 483, 720
BstSLI	GKGCMC	1 cut(s)	799
BstSNI	TACGTA	1 cut(s)	1811
BstV1I	GCAGC	8 cut(s)	33, 442, 497, 500, 788, 901, 1183, 1342
BstV2I	GAAGAC	1 cut(s)	286
BstX2I	RGATCY	6 cut(s)	4, 924, 1767, 1803, 1870, 2036
BstYI	RGATCY	6 cut(s)	4, 924, 1767, 1803, 1870, 2036
BstZ17I	GTATAC	1 cut(s)	1640
BsuRI	GGCC	4 cut(s)	630, 735, 1097, 1335
BtgI	CCRYGG	2 cut(s)	633, 980
BtgZI	GCGATG	1 cut(s)	982
BtsCI	GGATG	7 cut(s)	879, 959, 1206, 1254, 1389, 1915, 1931
BtsIMutI	CAGTG	2 cut(s)	778, 1686
BveI	ACCTGC	1 cut(s)	482
Cac8I	GCNNGC	4 cut(s)	977, 1003, 1279, 1647
CaiI	CAGNNNCTG	2 cut(s)	455, 497
CciI	TCATGA	1 cut(s)	901
Cfr13I	GGNCC	5 cut(s)	629, 1456, 1598, 1656, 1737
CseI	GACGC	2 cut(s)	791, 1390
Csp6I	GTAC	7 cut(s)	361, 419, 692, 707, 712, 763, 2018
CspCI	CAANNNNNGTGG	2 cut(s)	1458, 1493
CviQI	GTAC	7 cut(s)	361, 419, 692, 707, 712, 763, 2018
DdeI	CTNAG	6 cut(s)	87, 131, 156, 392, 695, 2000
DpnI	GATC	9 cut(s)	6, 143, 926, 1014, 1769, 1805, 1846, 1872, 2038
DpnII	GATC	9 cut(s)	4, 141, 924, 1012, 1767, 1803, 1844, 1870, 2036
DraI	TTTAAA	1 cut(s)	525
DrdI	GACNNNNNNGTC	1 cut(s)	1995
DseDI	GACNNNNNNGTC	1 cut(s)	1995
Eam1104I	CTCTTC	5 cut(s)	655, 1794, 1878, 1884, 2102
EarI	CTCTTC	5 cut(s)	655, 1794, 1878, 1884, 2102
EciI	GGCGGA	1 cut(s)	656
Eco105I	TACGTA	1 cut(s)	1811
Eco130I	CCWWGG	6 cut(s)	633, 980, 1210, 1366, 1836, 2029
Eco147I	AGGCCT	1 cut(s)	1335
Eco31I	GGTCTC	1 cut(s)	2003
Eco47I	GGWCC	4 cut(s)	1456, 1598, 1656, 1737
Eco88I	CYCGRG	1 cut(s)	1712
Eco91I	GGTNACC	1 cut(s)	1606
EcoO109I	RGGNCCY	2 cut(s)	629, 1737
EcoO65I	GGTNACC	1 cut(s)	1606
EcoRI	GAATTC	2 cut(s)	1163, 2052
EcoRII	CCWGG	1 cut(s)	1443
EcoT14I	CCWWGG	6 cut(s)	633, 980, 1210, 1366, 1836, 2029
EcoT22I	ATGCAT	3 cut(s)	541, 1825, 1933
ErhI	CCWWGG	6 cut(s)	633, 980, 1210, 1366, 1836, 2029
FaqI	GGGAC	3 cut(s)	1723, 1869, 2106
FauI	CCCGC	1 cut(s)	1834
FauNDI	CATATG	1 cut(s)	1225
FblI	GTMKAC	1 cut(s)	1639
FokI	GGATG	7 cut(s)	866, 946, 1193, 1241, 1396, 1902, 1918
FspBI	CTAG	4 cut(s)	809, 1268, 1964, 2125
HaeIII	GGCC	4 cut(s)	630, 735, 1097, 1335
HapII	CCGG	3 cut(s)	506, 606, 1272
HgaI	GACGC	2 cut(s)	791, 1390
Hin1I	GRCGYC	2 cut(s)	768, 956
HincII	GTYRAC	2 cut(s)	871, 1909
HindII	GTYRAC	2 cut(s)	871, 1909
HindIII	AAGCTT	2 cut(s)	516, 1060
HinfI	GANTC	4 cut(s)	1310, 1674, 1857, 2094
HpaII	CCGG	3 cut(s)	506, 606, 1272
HphI	GGTGA	2 cut(s)	931, 1459
Hpy166II	GTNNAC	4 cut(s)	871, 1204, 1640, 1909
Hpy8I	GTNNAC	4 cut(s)	871, 1204, 1640, 1909
Hpy99I	CGWCG	3 cut(s)	770, 1124, 2051
HpyAV	CCTTC	6 cut(s)	73, 620, 740, 1017, 1251, 1621
HpyCH4IV	ACGT	4 cut(s)	435, 768, 956, 1810
HpyF3I	CTNAG	6 cut(s)	87, 131, 156, 392, 695, 2000
HpySE526I	ACGT	4 cut(s)	435, 768, 956, 1810
Hsp92I	GRCGYC	2 cut(s)	768, 956
Kpn2I	TCCGGA	1 cut(s)	505
KpnI	GGTACC	1 cut(s)	2021
Kzo9I	GATC	9 cut(s)	4, 141, 924, 1012, 1767, 1803, 1844, 1870, 2036
LguI	GCTCTTC	1 cut(s)	655
LmnI	GCTCC	6 cut(s)	191, 484, 559, 922, 1274, 1352
Lsp1109I	GCAGC	8 cut(s)	33, 442, 497, 500, 788, 901, 1183, 1342
LweI	GCATC	6 cut(s)	526, 903, 1005, 1114, 1810, 1940
MaeI	CTAG	4 cut(s)	809, 1268, 1964, 2125
MaeII	ACGT	4 cut(s)	435, 768, 956, 1810
MaeIII	GTNAC	5 cut(s)	50, 317, 1021, 1606, 1959
MalI	GATC	9 cut(s)	6, 143, 926, 1014, 1769, 1805, 1846, 1872, 2038
MboI	GATC	9 cut(s)	4, 141, 924, 1012, 1767, 1803, 1844, 1870, 2036
MboII	GAAGA	9 cut(s)	286, 380, 642, 1180, 1811, 1880, 1895, 1901, 2089
MfeI	CAATTG	1 cut(s)	687
MflI	RGATCY	6 cut(s)	4, 924, 1767, 1803, 1870, 2036
MhlI	GDGCHC	3 cut(s)	650, 799, 2085
MluCI	AATT	9 cut(s)	29, 61, 231, 687, 840, 1163, 1215, 1500, 2052
MlyI	GAGTC	2 cut(s)	1851, 2088
MmeI	TCCRAC	2 cut(s)	54, 2029
Mph1103I	ATGCAT	3 cut(s)	541, 1825, 1933
MroI	TCCGGA	1 cut(s)	505
MroXI	GAANNNNTTC	2 cut(s)	126, 665
MseI	TTAA	5 cut(s)	524, 1491, 1518, 1733, 1875
MslI	CAYNNNNRTG	1 cut(s)	1382
MspA1I	CMGCKG	2 cut(s)	24, 779
MspI	CCGG	3 cut(s)	506, 606, 1272
MspR9I	CCNGG	1 cut(s)	1445
MunI	CAATTG	1 cut(s)	687
MvaI	CCWGG	1 cut(s)	1445
NcoI	CCATGG	2 cut(s)	633, 980
NdeI	CATATG	1 cut(s)	1225
NdeII	GATC	9 cut(s)	4, 141, 924, 1012, 1767, 1803, 1844, 1870, 2036
NlaIV	GGNNCC	8 cut(s)	6, 175, 555, 586, 631, 1375, 1739, 2019
NmuCI	GTSAC	3 cut(s)	50, 1021, 1959
NsiI	ATGCAT	3 cut(s)	541, 1825, 1933
NspI	RCATGY	2 cut(s)	1005, 1649
PaeI	GCATGC	2 cut(s)	1005, 1649
PagI	TCATGA	1 cut(s)	901
PceI	AGGCCT	1 cut(s)	1335
PciSI	GCTCTTC	1 cut(s)	655
PdmI	GAANNNNTTC	2 cut(s)	126, 665
PfeI	GAWTC	2 cut(s)	1310, 1674
PflFI	GACNNNGTC	2 cut(s)	875, 1957
PleI	GAGTC	2 cut(s)	1851, 2088
PpsI	GAGTC	2 cut(s)	1851, 2088
Ppu21I	YACGTR	1 cut(s)	1811
PpuMI	RGGWCCY	1 cut(s)	1737
Psp5II	RGGWCCY	1 cut(s)	1737
Psp6I	CCWGG	1 cut(s)	1443
PspEI	GGTNACC	1 cut(s)	1606
PspGI	CCWGG	1 cut(s)	1443
PspN4I	GGNNCC	8 cut(s)	6, 175, 555, 586, 631, 1375, 1739, 2019
PspPI	GGNCC	5 cut(s)	629, 1456, 1598, 1656, 1737
PspPPI	RGGWCCY	1 cut(s)	1737
PstI	CTGCAG	1 cut(s)	487
PstNI	CAGNNNCTG	2 cut(s)	455, 497
PsuI	RGATCY	6 cut(s)	4, 924, 1767, 1803, 1870, 2036
PsyI	GACNNNGTC	2 cut(s)	875, 1957
PvuII	CAGCTG	1 cut(s)	779
RsaI	GTAC	7 cut(s)	362, 420, 693, 708, 713, 764, 2019
RsaNI	GTAC	7 cut(s)	361, 419, 692, 707, 712, 763, 2018
RseI	CAYNNNNRTG	1 cut(s)	1382
SapI	GCTCTTC	1 cut(s)	655
SaqAI	TTAA	5 cut(s)	524, 1491, 1518, 1733, 1875
Sau3AI	GATC	9 cut(s)	4, 141, 924, 1012, 1767, 1803, 1844, 1870, 2036
Sau96I	GGNCC	5 cut(s)	629, 1456, 1598, 1656, 1737
ScaI	AGTACT	2 cut(s)	420, 713
SchI	GAGTC	2 cut(s)	1851, 2088
ScrFI	CCNGG	1 cut(s)	1445
SduI	GDGCHC	3 cut(s)	650, 799, 2085
SfaNI	GCATC	6 cut(s)	526, 903, 1005, 1114, 1810, 1940
SfcI	CTRYAG	3 cut(s)	115, 483, 720
SinI	GGWCC	4 cut(s)	1456, 1598, 1656, 1737
SmiI	ATTTAAAT	1 cut(s)	525
SmiMI	CAYNNNNRTG	1 cut(s)	1382
SmlI	CTYRAG	2 cut(s)	56, 364
SmoI	CTYRAG	2 cut(s)	56, 364
SnaBI	TACGTA	1 cut(s)	1811
SphI	GCATGC	2 cut(s)	1005, 1649
Sse9I	AATT	9 cut(s)	29, 61, 231, 687, 840, 1163, 1215, 1500, 2052
SseBI	AGGCCT	1 cut(s)	1335
SsiI	CCGC	8 cut(s)	24, 125, 458, 641, 750, 1381, 1841, 1974
SspI	AATATT	2 cut(s)	269, 1879
SspMI	CTAG	4 cut(s)	809, 1268, 1964, 2125
StuI	AGGCCT	1 cut(s)	1335
StyD4I	CCNGG	1 cut(s)	1443
StyI	CCWWGG	6 cut(s)	633, 980, 1210, 1366, 1836, 2029
SwaI	ATTTAAAT	1 cut(s)	525
TaiI	ACGT	4 cut(s)	438, 771, 959, 1813
TaqI	TCGA	3 cut(s)	1326, 1623, 1987
TasI	AATT	9 cut(s)	29, 61, 231, 687, 840, 1163, 1215, 1500, 2052
TatI	WGTACW	3 cut(s)	418, 691, 711
TauI	GCSGC	2 cut(s)	461, 1976
TfiI	GAWTC	2 cut(s)	1310, 1674
Tru1I	TTAA	5 cut(s)	524, 1491, 1518, 1733, 1875
Tru9I	TTAA	5 cut(s)	524, 1491, 1518, 1733, 1875
TscAI	CASTG	2 cut(s)	778, 1686
TseFI	GTSAC	3 cut(s)	50, 1021, 1959
TseI	GCWGC	8 cut(s)	21, 455, 485, 488, 776, 914, 1196, 1355
Tsp45I	GTSAC	3 cut(s)	50, 1021, 1959
TspRI	CASTG	2 cut(s)	778, 1686
Tth111I	GACNNNGTC	2 cut(s)	875, 1957
VpaK11BI	GGWCC	4 cut(s)	1456, 1598, 1656, 1737
XapI	RAATTY	5 cut(s)	61, 840, 1163, 1500, 2052
XceI	RCATGY	2 cut(s)	1005, 1649
XmiI	GTMKAC	1 cut(s)	1639
XmnI	GAANNNNTTC	2 cut(s)	126, 665
XspI	CTAG	4 cut(s)	809, 1268, 1964, 2125
ZraI	GACGTC	2 cut(s)	769, 957
ZrmI	AGTACT	2 cut(s)	420, 713
Zsp2I	ATGCAT	3 cut(s)	541, 1825, 1933

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.