FvH4_1g29290

Phosphatidylinositol 4-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014385
Physical Location & Seq
Forward (+)
22207013 .. 22209734
2722 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29290.t1

Sequence Viewer

Length: 1626 bp
ATGAGATTCGTGGAAATGGCTGTCGCAGTTGATCAACATGACGGAATCAAGCCATTAAGCAAATCCCTGACATGTAGATTACAATCCTACACCCATCTTGATTACAACATATTTTCCTCCTCCTTTAAAAATACATGCGGAGTTGCAAACATTCACAAAAGCTTTTCCACCCCATGTCTCTCCCGAACTAGTGCATTGGAGGAAGACTTTGATACTAATCCAAGAATTGAGATTGTTGGTGGTAGCAGAGTTCCTAGGGTACATGCTCTTGTAGTTGAGGTCGCCATAGCTATGGCTTCTGGTGCTGATCTATTGCCAGTGCCAAGTGGACTAGGAGGGGCCTACTTCTTATGTAGTCGAAATGGTGACAATATTGCTGTAGCAAAGCCCATAGATGAAGAACCCTTAGCCTTTAATAATCCTAAAGGCTTCGGAGGTAGGATGCTTGGCCAACCTGGTTTAAAACGCTCAGTTCGGATTGGTGAATCTGGAGTTCGAGAATTGGCAGCTTACCTCCTTGACCATGGTGGTTTCGCTGGTGTTCCTCCAACCGCTTTAGTAAAAATTTCTCATGTTGCATTCCGTGTTAATAATAATTCAGCTGCAATTTCAACTACACCCTACAAGATTTCTTCTCTTCAGCGCTTTGTAGATCATGACTTTGATGCAGGAGAATTGGGTTTTTCTGGCTTCTCAATAGCTTCAGTTCATCACATTGGAATTTTTGACGTAAGAGTGCTAAATCTTGATAGGCACGCAGGTAATATGCTTGTTAATAAGTATGAGCATAACAATTATACCGTTGGTGTGGCTGAGCTTGTGCCTATTGACCACGGGCTTTGCCTTCCTGAGTGGCTTGATGATCCATACTTTGAATGGTTGCACTGGCCTCAAGCTTCAGTTCCATTTTCTGAGTCCGAGATCGAGTACATATCCAATCTTGACCCATCTAAAGATGCGGAGCTTCTACGAACTCAAATTCCTTCTTTACGTGAGGCTGCTGTCCGTGTTCTTGTGCTTTGCACCATCTTCTTGAAGCGGGCTACAGCAGCTGGGCTTTGTCTTGCTGATATAGGTGAAATGATGACTCGACAGTTTTGTGCTGGCGAAGAAAGATTGAGTGCATTAGAGAATCTTTGCTTACAAGTTAAAGCTACAATGTCGGGTACAAATGACAAAAAAACAGAAACAACCAAAGGAGGTGAAATATTTCAATTCCAGAGTGATACCAAGGAAAGTTTTGATGAGAGTTTTGATCTTCCTCGACTCTTACAGATTTATCCTGGAATGTCAAGGTTTTCATCAGTGGGATCAATGCCCGATGCATATATCTATCAATTATTTAAGGAACGCAATCGTGACAATGATGATTATGATATGAAAGGCAATAATGCTTATGTTGGTGGTAGTACTAGAGATTGTCATAAGGTAGGGGGTTTGATGGAGAGCGTGAGTTTCTCAGTAAATAACTTCAATTGTGAAACAGGGGCAATATCTTTCGGAGACATGAGTAAAGCTGAATGGGCTTTGTTCTTGGACAATTTTGAAAAGCTTTTGCCCGGCATCCTCAAGGGCACAAACTACTTGAGCTTGAGGACAACATTGAAAACTTCCTGCAAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

542

Amino Acids

59.57

Weight (kDa)

5.66

Isoelectric Point (pI)

37.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 125 - 357 5.3e-41 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014385)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40850 AT3G56600 AT3G56600 AT3G56600
fragaria_vesca FvH4_1g29290
malus_domestica MD00G1140900.v1.1
prunus_persica Prupe.7G019100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0494791
rosa_laevigata RLG00000022662
rosa_multiflora Rmu_sc0000198.1_g000015
rosa_roxburghii Rroxscaffold_6G00390900
rosa_rugosa Rorug03G0268200
rosa_samantha Rh3AG317000 Rh3BG352200 Rh3CG349200 Rh3DG351600
rosa_wichuraiana Rw3G027650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 749
AciI CCGC 4 cut(s) 138, 552, 959, 1039
AclWI GGATC 2 cut(s) 857, 1318
AcoI YGGCCR 1 cut(s) 448
AcsI RAATTY 3 cut(s) 564, 720, 978
AcuI CTGAAG 3 cut(s) 623, 687, 882
AfaI GTAC 4 cut(s) 261, 929, 1168, 1411
AfeI AGCGCT 1 cut(s) 644
AflIII ACRYGT 1 cut(s) 71
AgsI TTSAA 7 cut(s) 612, 875, 1036, 1214, 1474, 1547, 1606
AhlI ACTAGT 1 cut(s) 188
AjnI CCWGG 2 cut(s) 454, 1282
AloI GAACNNNNNNTCC 2 cut(s) 964, 996
Alw26I GTCTC 2 cut(s) 182, 1497
AlwI GGATC 2 cut(s) 857, 1318
AlwNI CAGNNNCTG 1 cut(s) 1052
Aor51HI AGCGCT 1 cut(s) 644
AoxI GGCC 3 cut(s) 339, 448, 887
ApeKI GCWGC 4 cut(s) 506, 602, 998, 1049
ApoI RAATTY 3 cut(s) 564, 720, 978
Asp700I GAANNNNTTC 1 cut(s) 1209
AspA2I CCTAGG 1 cut(s) 254
AspLEI GCGC 1 cut(s) 645
AspS9I GGNCC 1 cut(s) 339
AsuC2I CCSGG 1 cut(s) 1560
AsuHPI GGTGA 4 cut(s) 377, 494, 1088, 1214
AvrII CCTAGG 1 cut(s) 254
BaeGI GKGCMC 1 cut(s) 1577
BalI TGGCCA 1 cut(s) 450
BbsI GAAGAC 1 cut(s) 210
BbvI GCAGC 4 cut(s) 518, 589, 985, 1061
BccI CCATC 4 cut(s) 102, 955, 1034, 1435
BciT130I CCWGG 2 cut(s) 456, 1284
BclI TGATCA 1 cut(s) 31
BcnI CCSGG 1 cut(s) 1560
BcoDI GTCTC 2 cut(s) 182, 1497
BcuI ACTAGT 1 cut(s) 188
BfaI CTAG 4 cut(s) 189, 255, 332, 1413
BfmI CTRYAG 2 cut(s) 378, 1044
BfoI RGCGCY 1 cut(s) 646
BfuAI ACCTGC 1 cut(s) 749
BisI GCNGC 4 cut(s) 507, 603, 999, 1050
BlnI CCTAGG 1 cut(s) 254
BlpI GCTNAGC 1 cut(s) 813
BlsI GCNGC 4 cut(s) 508, 604, 1000, 1051
BmcAI AGTACT 1 cut(s) 1411
Bme1390I CCNGG 3 cut(s) 456, 1284, 1560
BmgT120I GGNCC 1 cut(s) 339
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 3 cut(s) 456, 1284, 1560
BmsI GCATC 5 cut(s) 432, 655, 946, 1312, 1572
BpiI GAAGAC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 406
Bpu1102I GCTNAGC 1 cut(s) 813
BpuEI CTTGAG 4 cut(s) 876, 1553, 1606, 1612
BpuMI CCSGG 1 cut(s) 1560
BsaAI YACGTR 1 cut(s) 992
BsaBI GATNNNNATC 2 cut(s) 82, 216
BsaJI CCNNGG 4 cut(s) 254, 523, 832, 1230
Bse1I ACTGG 2 cut(s) 317, 890
Bse8I GATNNNNATC 2 cut(s) 82, 216
BseBI CCWGG 2 cut(s) 456, 1284
BseDI CCNNGG 4 cut(s) 254, 523, 832, 1230
BseGI GGATG 2 cut(s) 447, 1563
BseJI GATNNNNATC 2 cut(s) 82, 216
BseMII CTCAG 5 cut(s) 483, 804, 840, 903, 1473
BseNI ACTGG 2 cut(s) 317, 890
BseRI GAGGAG 1 cut(s) 109
BseSI GKGCMC 1 cut(s) 1577
BseXI GCAGC 4 cut(s) 518, 589, 985, 1061
BseYI CCCAGC 1 cut(s) 1052
BshFI GGCC 3 cut(s) 341, 450, 889
BsiSI CCGG 1 cut(s) 1560
BsmAI GTCTC 2 cut(s) 182, 1497
BsmI GAATGC 1 cut(s) 578
BsnI GGCC 3 cut(s) 341, 450, 889
Bsp1286I GDGCHC 1 cut(s) 1577
Bsp143I GATC 7 cut(s) 31, 307, 652, 862, 921, 1255, 1310
Bsp1720I GCTNAGC 1 cut(s) 813
Bsp19I CCATGG 1 cut(s) 523
BspACI CCGC 4 cut(s) 138, 552, 959, 1039
BspANI GGCC 3 cut(s) 341, 450, 889
BspCNI CTCAG 5 cut(s) 482, 805, 841, 904, 1472
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 1 cut(s) 340
BspMI ACCTGC 1 cut(s) 749
BspPI GGATC 2 cut(s) 857, 1318
BsrI ACTGG 2 cut(s) 317, 890
BssECI CCNNGG 4 cut(s) 254, 523, 832, 1230
BssMI GATC 7 cut(s) 31, 307, 652, 862, 921, 1255, 1310
BssT1I CCWWGG 3 cut(s) 254, 523, 1230
Bst2UI CCWGG 2 cut(s) 456, 1284
Bst4CI ACNGT 2 cut(s) 802, 1095
Bst6I CTCTTC 1 cut(s) 642
BstBAI YACGTR 1 cut(s) 992
BstC8I GCNNGC 3 cut(s) 756, 1041, 1105
BstDEI CTNAG 6 cut(s) 406, 469, 813, 849, 912, 1459
BstDSI CCRYGG 2 cut(s) 523, 832
BstF5I GGATG 2 cut(s) 447, 1563
BstH2I RGCGCY 1 cut(s) 646
BstHHI GCGC 1 cut(s) 645
BstKTI GATC 7 cut(s) 34, 310, 655, 865, 924, 1258, 1313
BstMAI GTCTC 2 cut(s) 182, 1497
BstMBI GATC 7 cut(s) 31, 307, 652, 862, 921, 1255, 1310
BstMWI GCNNNNNNNGC 3 cut(s) 302, 1049, 1523
BstNI CCWGG 2 cut(s) 456, 1284
BstNSI RCATGY 3 cut(s) 75, 138, 266
BstSCI CCNGG 3 cut(s) 454, 1282, 1558
BstSFI CTRYAG 2 cut(s) 378, 1044
BstSLI GKGCMC 1 cut(s) 1577
BstV1I GCAGC 4 cut(s) 518, 589, 985, 1061
BstV2I GAAGAC 1 cut(s) 210
BstXI CCANNNNNNTGG 1 cut(s) 292
BsuRI GGCC 3 cut(s) 341, 450, 889
BtgI CCRYGG 2 cut(s) 523, 832
BtsCI GGATG 2 cut(s) 447, 1563
BtsIMutI CAGTG 3 cut(s) 324, 883, 1311
BveI ACCTGC 1 cut(s) 749
Cac8I GCNNGC 3 cut(s) 756, 1041, 1105
CaiI CAGNNNCTG 1 cut(s) 1052
CciI TCATGA 1 cut(s) 655
CfoI GCGC 1 cut(s) 645
Cfr13I GGNCC 1 cut(s) 339
CsiI ACCWGGT 1 cut(s) 454
Csp6I GTAC 4 cut(s) 260, 928, 1167, 1410
CspCI CAANNNNNGTGG 2 cut(s) 821, 856
CviAII CATG 9 cut(s) 38, 72, 135, 174, 263, 524, 572, 656, 1507
CviQI GTAC 4 cut(s) 260, 928, 1167, 1410
DdeI CTNAG 6 cut(s) 406, 469, 813, 849, 912, 1459
DpnI GATC 7 cut(s) 33, 309, 654, 864, 923, 1257, 1312
DpnII GATC 7 cut(s) 31, 307, 652, 862, 921, 1255, 1310
DraI TTTAAA 2 cut(s) 127, 462
EaeI YGGCCR 1 cut(s) 448
Eam1104I CTCTTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 642
Eco130I CCWWGG 3 cut(s) 254, 523, 1230
Eco47III AGCGCT 1 cut(s) 644
Eco57I CTGAAG 3 cut(s) 623, 687, 882
EcoO109I RGGNCCY 1 cut(s) 339
EcoRII CCWGG 2 cut(s) 454, 1282
EcoT14I CCWWGG 3 cut(s) 254, 523, 1230
EcoT22I ATGCAT 1 cut(s) 1327
ErhI CCWWGG 3 cut(s) 254, 523, 1230
FaeI CATG 9 cut(s) 41, 75, 138, 177, 266, 527, 575, 659, 1510
FatI CATG 9 cut(s) 37, 71, 134, 173, 262, 523, 571, 655, 1506
FauI CCCGC 1 cut(s) 1032
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 4 cut(s) 507, 603, 999, 1050
FokI GGATG 2 cut(s) 454, 1550
Fsp4HI GCNGC 4 cut(s) 507, 603, 999, 1050
FspBI CTAG 4 cut(s) 189, 255, 332, 1413
GlaI GCGC 1 cut(s) 644
GluI GCNGC 4 cut(s) 507, 603, 999, 1050
GsaI CCCAGC 1 cut(s) 1056
GsuI CTGGAG 1 cut(s) 510
HaeII RGCGCY 1 cut(s) 646
HaeIII GGCC 3 cut(s) 341, 450, 889
HapII CCGG 1 cut(s) 1560
HhaI GCGC 1 cut(s) 645
Hin1II CATG 9 cut(s) 41, 75, 138, 177, 266, 527, 575, 659, 1510
Hin6I GCGC 1 cut(s) 643
HinP1I GCGC 1 cut(s) 643
HindIII AAGCTT 3 cut(s) 160, 894, 1550
HinfI GANTC 7 cut(s) 6, 45, 485, 914, 1087, 1132, 1266
HpaII CCGG 1 cut(s) 1560
HphI GGTGA 4 cut(s) 377, 494, 1088, 1214
Hpy166II GTNNAC 1 cut(s) 329
Hpy188I TCNGA 5 cut(s) 434, 477, 913, 919, 1502
Hpy8I GTNNAC 1 cut(s) 329
HpyAV CCTTC 2 cut(s) 854, 993
HpyCH4III ACNGT 2 cut(s) 802, 1095
HpyCH4IV ACGT 2 cut(s) 729, 991
HpyF10VI GCNNNNNNNGC 3 cut(s) 302, 1049, 1523
HpyF3I CTNAG 6 cut(s) 406, 469, 813, 849, 912, 1459
HpySE526I ACGT 2 cut(s) 729, 991
Hsp92II CATG 9 cut(s) 41, 75, 138, 177, 266, 527, 575, 659, 1510
HspAI GCGC 1 cut(s) 643
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 7 cut(s) 31, 307, 652, 862, 921, 1255, 1310
LmnI GCTCC 1 cut(s) 961
Lsp1109I GCAGC 4 cut(s) 518, 589, 985, 1061
LweI GCATC 5 cut(s) 432, 655, 946, 1312, 1572
MabI ACCWGGT 1 cut(s) 454
MaeI CTAG 4 cut(s) 189, 255, 332, 1413
MaeII ACGT 2 cut(s) 729, 991
MaeIII GTNAC 2 cut(s) 365, 1358
MalI GATC 7 cut(s) 33, 309, 654, 864, 923, 1257, 1312
MboI GATC 7 cut(s) 31, 307, 652, 862, 921, 1255, 1310
MboII GAAGA 7 cut(s) 215, 410, 624, 629, 1021, 1121, 1250
MfeI CAATTG 1 cut(s) 1474
MhlI GDGCHC 1 cut(s) 1577
MlsI TGGCCA 1 cut(s) 450
MluNI TGGCCA 1 cut(s) 450
MlyI GAGTC 3 cut(s) 923, 1081, 1260
MmeI TCCRAC 1 cut(s) 572
Mox20I TGGCCA 1 cut(s) 450
Mph1103I ATGCAT 1 cut(s) 1327
MroXI GAANNNNTTC 1 cut(s) 1209
MscI TGGCCA 1 cut(s) 450
MseI TTAA 8 cut(s) 56, 126, 414, 461, 588, 774, 1149, 1344
MslI CAYNNNNRTG 1 cut(s) 290
Msp20I TGGCCA 1 cut(s) 450
MspA1I CMGCKG 2 cut(s) 602, 1052
MspI CCGG 1 cut(s) 1560
MspR9I CCNGG 3 cut(s) 456, 1284, 1560
MunI CAATTG 1 cut(s) 1474
Mva1269I GAATGC 1 cut(s) 578
MvaI CCWGG 2 cut(s) 456, 1284
MwoI GCNNNNNNNGC 3 cut(s) 302, 1049, 1523
NciI CCSGG 1 cut(s) 1560
NcoI CCATGG 1 cut(s) 523
NdeII GATC 7 cut(s) 31, 307, 652, 862, 921, 1255, 1310
NlaIII CATG 9 cut(s) 41, 75, 138, 177, 266, 527, 575, 659, 1510
NlaIV GGNNCC 1 cut(s) 340
NmuCI GTSAC 2 cut(s) 365, 1358
NsiI ATGCAT 1 cut(s) 1327
NspI RCATGY 3 cut(s) 75, 138, 266
PagI TCATGA 1 cut(s) 655
PciI ACATGT 1 cut(s) 71
PctI GAATGC 1 cut(s) 578
PdmI GAANNNNTTC 1 cut(s) 1209
PfeI GAWTC 4 cut(s) 6, 45, 485, 1132
PfoI TCCNGGA 1 cut(s) 1282
PkrI GCNGC 4 cut(s) 508, 604, 1000, 1051
PleI GAGTC 3 cut(s) 922, 1081, 1260
PpsI GAGTC 3 cut(s) 922, 1081, 1260
Ppu21I YACGTR 1 cut(s) 992
PscI ACATGT 1 cut(s) 71
Psp6I CCWGG 2 cut(s) 454, 1282
PspFI CCCAGC 1 cut(s) 1052
PspGI CCWGG 2 cut(s) 454, 1282
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 1 cut(s) 339
PstNI CAGNNNCTG 1 cut(s) 1052
PvuII CAGCTG 2 cut(s) 602, 1052
RsaI GTAC 4 cut(s) 261, 929, 1168, 1411
RsaNI GTAC 4 cut(s) 260, 928, 1167, 1410
RseI CAYNNNNRTG 1 cut(s) 290
SaqAI TTAA 8 cut(s) 56, 126, 414, 461, 588, 774, 1149, 1344
SatI GCNGC 4 cut(s) 507, 603, 999, 1050
Sau3AI GATC 7 cut(s) 31, 307, 652, 862, 921, 1255, 1310
Sau96I GGNCC 1 cut(s) 339
ScaI AGTACT 1 cut(s) 1411
SchI GAGTC 3 cut(s) 923, 1081, 1260
ScrFI CCNGG 3 cut(s) 456, 1284, 1560
SduI GDGCHC 1 cut(s) 1577
SexAI ACCWGGT 1 cut(s) 454
SfaNI GCATC 5 cut(s) 432, 655, 946, 1312, 1572
SfcI CTRYAG 2 cut(s) 378, 1044
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 4 cut(s) 891, 1568, 1585, 1591
SmoI CTYRAG 4 cut(s) 891, 1568, 1585, 1591
SpeI ACTAGT 1 cut(s) 188
SsiI CCGC 4 cut(s) 138, 552, 959, 1039
SspI AATATT 2 cut(s) 373, 1209
SspMI CTAG 4 cut(s) 189, 255, 332, 1413
StyD4I CCNGG 3 cut(s) 454, 1282, 1558
StyI CCWWGG 3 cut(s) 254, 523, 1230
TaaI ACNGT 2 cut(s) 802, 1095
TaiI ACGT 2 cut(s) 732, 994
TaqI TCGA 5 cut(s) 358, 496, 924, 1090, 1264
TatI WGTACW 2 cut(s) 927, 1409
TfiI GAWTC 4 cut(s) 6, 45, 485, 1132
Tru1I TTAA 8 cut(s) 56, 126, 414, 461, 588, 774, 1149, 1344
Tru9I TTAA 8 cut(s) 56, 126, 414, 461, 588, 774, 1149, 1344
TscAI CASTG 3 cut(s) 324, 890, 1311
TseFI GTSAC 2 cut(s) 365, 1358
TseI GCWGC 4 cut(s) 506, 602, 998, 1049
Tsp45I GTSAC 2 cut(s) 365, 1358
TspDTI ATGAA 4 cut(s) 411, 698, 1290, 1394
TspGWI ACGGA 3 cut(s) 57, 572, 995
TspRI CASTG 3 cut(s) 324, 890, 1311
XapI RAATTY 3 cut(s) 564, 720, 978
XceI RCATGY 3 cut(s) 75, 138, 266
XcmI CCANNNNNNNNNTGG 1 cut(s) 873
XmaJI CCTAGG 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 1209
XspI CTAG 4 cut(s) 189, 255, 332, 1413
ZrmI AGTACT 1 cut(s) 1411
Zsp2I ATGCAT 1 cut(s) 1327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.