Rroxscaffold_6G00390900

Phosphatidylinositol 4-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0014385
Physical Location & Seq
Reverse (-)
6866332 .. 6867962
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00390900.1

Sequence Viewer

Length: 1608 bp
ATGGCTGTAGCAGTTGATCAGCATCACGGAATCAAGCCATTAAGCCGATCCCAGAGATGCAGATTACAATCCTACACCCATCTTGATTACAGCATATTTCCCCACTCCTTTATAAATACATGCGGAGTTGCTAATATTCACAAAAGCTTTTCCACCCCATGTCTCTCCCAAACTACTGCACTGGAGGACGACTTTGACTCTAATCCACGGATTGAGATTGTTGGTGGTAGCAGAATTCCTAGGGTACATGCTCTTGTGGTTGAGGTCGCCATAGCTATGGCTTCCGGTGCTGATCTATTGCCAGTGACAAGCGGACTAGGGGGGCCTACTTCTTATGTAGTCGAAATGCCCATAGATGAAGAACCTTTAGCCTTCAATAATCCTAAAGGCTTCGGAGGTAGGATGCTGGGCCAACCTGGTTTAAAACGCTCAGTTCGGATTGGTGAATCTGGAATCCGAGAATTAGCAGCTTATATCCTTGACCATGGTGGATTCGCTGGTGTTCCTCCAACTGCTTTAGTAAAAATTTCTCATGTTGCATTCCGCGTTAATAATAATGCACCTACAATTTCTGCTACTCCCTACAAGATTGCTTCTCTTCAGCGGTTTGTAGATCATGATTTTGATGCAGGAGAATTGGGTTTCTCTGGCTTCTCAATAGCTTCAGTTCATCACATTGGAATTTTTGATGTAAGAGTCCTAAATCTTGATAGGCACGCAGGTAATATGCTTGTTAAGAAGTATAAGCAGAACAACTATAAAGTTGGTGTGGCTGAGCTTGTGCCTATTGACCATGGGCTTTGCCTTCCTGAGTGGCTTGATGATCCATACTTTGAATGGCTGCACTGGCCTCAAGCTTCAATTCCATTTTCTGAGTCTGAGATTGAGTACATATCAAATCTCAATCCCTCTAAAGATGCGGAGCTTTTACGAGCTCAGATTCCTTCTTTACGTGAGGCTGCTATCCGTGTTCTTGTGCTCTGCACCATCTTCTTGAAGCGGGCTACAGCTGCTGGGCTTTGTCTTGCTGATATAGGTGAAATGATGACTCGACAATTCTGCGCTGGTGAAGAAAGATTGAGTACATTAGAAAATCTTTGCTTACAAGTTAAAGCTACAATGTCTGGTACAGATGAGAAAAACAGAGAAACAATTGGAGAAGGTGAAATATTTCAACTTCACAATGATATTAATGAAAGTTTCGATGAGAATTTTGATCTTCCTCAACTCTTACAGATCTATCCTGGAATGTCAAGGTTTTCATCTGTAGGATCAATTACTGAGTTGACCGATGCATATATCTATCCACCATTTAAGGAAAGCAATCATGGCAATGATGATTCTGATAGTAAGAATATGAAAGGTAATAGTGGTTATGTTGATGGTAGTACTAGTGGCAGTCATAAGGTAGGGGGTCTGATGGAGAGAGTGAGTTTCTCAGTAAATAACTTCAATTGTGAAACAGGGGCAATTTCTTTTGGAGACATGAGTAAAGATGAATGGGATTTGTTCTTGGACAATTTTGAGAAGCTTTTGCCCGACATCCTCCTCAAGGGCACAAATTACTTGAGCTTGAAGAAAAGGTGGAGAACTTCCTGCAAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

535

Amino Acids

59.09

Weight (kDa)

5.54

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 117 - 344 7e-40 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014385)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40850 AT3G56600 AT3G56600 AT3G56600
fragaria_vesca FvH4_1g29290
malus_domestica MD00G1140900.v1.1
prunus_persica Prupe.7G019100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0494791
rosa_laevigata RLG00000022662
rosa_multiflora Rmu_sc0000198.1_g000015
rosa_roxburghii Rroxscaffold_6G00390900
rosa_rugosa Rorug03G0268200
rosa_samantha Rh3AG317000 Rh3BG352200 Rh3CG349200 Rh3DG351600
rosa_wichuraiana Rw3G027650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 113
Acc36I ACCTGC 1 cut(s) 710
AccII CGCG 1 cut(s) 546
AciI CCGC 6 cut(s) 123, 312, 544, 604, 920, 1000
AclWI GGATC 3 cut(s) 42, 818, 1279
AcsI RAATTY 4 cut(s) 234, 525, 681, 1210
AcuI CTGAAG 2 cut(s) 584, 648
AfaI GTAC 5 cut(s) 246, 890, 1084, 1129, 1390
AfiI CCNNNNNNNGG 1 cut(s) 1552
AgsI TTSAA 7 cut(s) 376, 836, 861, 997, 1175, 1453, 1576
AhlI ACTAGT 1 cut(s) 1391
AjnI CCWGG 2 cut(s) 415, 1243
Alw21I GWGCWC 2 cut(s) 937, 981
Alw26I GTCTC 2 cut(s) 167, 1476
AlwI GGATC 3 cut(s) 42, 818, 1279
AlwNI CAGNNNCTG 1 cut(s) 1013
AoxI GGCC 3 cut(s) 323, 409, 848
ApeKI GCWGC 4 cut(s) 467, 841, 959, 1010
ApoI RAATTY 4 cut(s) 234, 525, 681, 1210
AseI ATTAAT 1 cut(s) 1191
Asp700I GAANNNNTTC 1 cut(s) 1170
AspA2I CCTAGG 1 cut(s) 239
AspLEI GCGC 1 cut(s) 1064
AspS9I GGNCC 2 cut(s) 323, 409
AsuHPI GGTGA 4 cut(s) 455, 1049, 1079, 1175
AvrII CCTAGG 1 cut(s) 239
BaeGI GKGCMC 1 cut(s) 1559
BanII GRGCYC 1 cut(s) 937
Bbv12I GWGCWC 2 cut(s) 937, 981
BbvI GCAGC 4 cut(s) 479, 828, 946, 997
BccI CCATC 4 cut(s) 87, 995, 1376, 1414
BcgI CGANNNNNNTGC 2 cut(s) 1041, 1075
BciT130I CCWGG 2 cut(s) 417, 1245
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 2 cut(s) 167, 1476
BcuI ACTAGT 1 cut(s) 1391
BfaI CTAG 3 cut(s) 240, 317, 1392
BfmI CTRYAG 3 cut(s) 6, 1005, 1266
BfuAI ACCTGC 1 cut(s) 710
BglII AGATCT 1 cut(s) 1236
BisI GCNGC 4 cut(s) 468, 842, 960, 1011
BlnI CCTAGG 1 cut(s) 239
BlpI GCTNAGC 1 cut(s) 774
BlsI GCNGC 4 cut(s) 469, 843, 961, 1012
BmcAI AGTACT 1 cut(s) 1390
Bme1390I CCNGG 2 cut(s) 417, 1245
BmgT120I GGNCC 2 cut(s) 323, 409
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 2 cut(s) 417, 1245
BmsI GCATC 6 cut(s) 31, 47, 393, 616, 907, 1282
BpmI CTGGAG 1 cut(s) 203
Bpu1102I GCTNAGC 1 cut(s) 774
BpuEI CTTGAG 3 cut(s) 837, 1535, 1588
BsaAI YACGTR 1 cut(s) 953
BsaBI GATNNNNATC 2 cut(s) 21, 67
BsaJI CCNNGG 4 cut(s) 206, 239, 484, 793
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 1 cut(s) 1552
Bse1I ACTGG 3 cut(s) 186, 302, 851
Bse3DI GCAATG 1 cut(s) 1339
Bse8I GATNNNNATC 2 cut(s) 21, 67
BseBI CCWGG 2 cut(s) 417, 1245
BseDI CCNNGG 4 cut(s) 206, 239, 484, 793
BseGI GGATG 2 cut(s) 408, 1542
BseJI GATNNNNATC 2 cut(s) 21, 67
BseLI CCNNNNNNNGG 1 cut(s) 1552
BseMI GCAATG 1 cut(s) 1339
BseMII CTCAG 8 cut(s) 444, 765, 801, 864, 870, 950, 1272, 1452
BseNI ACTGG 3 cut(s) 186, 302, 851
BseRI GAGGAG 1 cut(s) 1538
BseSI GKGCMC 1 cut(s) 1559
BseXI GCAGC 4 cut(s) 479, 828, 946, 997
BseYI CCCAGC 2 cut(s) 406, 1013
BsgI GTGCAG 3 cut(s) 162, 827, 967
Bsh1236I CGCG 1 cut(s) 546
BshFI GGCC 3 cut(s) 325, 411, 850
BsiHKAI GWGCWC 2 cut(s) 937, 981
BsiSI CCGG 1 cut(s) 285
BslI CCNNNNNNNGG 1 cut(s) 1552
BsmAI GTCTC 2 cut(s) 167, 1476
BsmI GAATGC 1 cut(s) 539
BsnI GGCC 3 cut(s) 325, 411, 850
Bsp1286I GDGCHC 3 cut(s) 937, 981, 1559
Bsp143I GATC 8 cut(s) 16, 47, 292, 613, 823, 1216, 1236, 1271
Bsp1720I GCTNAGC 1 cut(s) 774
Bsp19I CCATGG 2 cut(s) 484, 793
BspACI CCGC 6 cut(s) 123, 312, 544, 604, 920, 1000
BspANI GGCC 3 cut(s) 325, 411, 850
BspCNI CTCAG 8 cut(s) 443, 766, 802, 865, 871, 949, 1273, 1451
BspFNI CGCG 1 cut(s) 546
BspHI TCATGA 1 cut(s) 616
BspLI GGNNCC 1 cut(s) 324
BspMI ACCTGC 1 cut(s) 710
BspPI GGATC 3 cut(s) 42, 818, 1279
BsrDI GCAATG 1 cut(s) 1339
BsrI ACTGG 3 cut(s) 186, 302, 851
BssECI CCNNGG 4 cut(s) 206, 239, 484, 793
BssMI GATC 8 cut(s) 16, 47, 292, 613, 823, 1216, 1236, 1271
BssT1I CCWWGG 3 cut(s) 239, 484, 793
Bst2UI CCWGG 2 cut(s) 417, 1245
Bst6I CTCTTC 1 cut(s) 603
BstBAI YACGTR 1 cut(s) 953
BstC8I GCNNGC 2 cut(s) 717, 1002
BstDEI CTNAG 8 cut(s) 430, 774, 810, 873, 879, 936, 1281, 1438
BstDSI CCRYGG 3 cut(s) 206, 484, 793
BstENI CCTNNNNNAGG 1 cut(s) 1550
BstF5I GGATG 2 cut(s) 408, 1542
BstFNI CGCG 1 cut(s) 546
BstHHI GCGC 1 cut(s) 1064
BstKTI GATC 8 cut(s) 19, 50, 295, 616, 826, 1219, 1239, 1274
BstMAI GTCTC 2 cut(s) 167, 1476
BstMBI GATC 8 cut(s) 16, 47, 292, 613, 823, 1216, 1236, 1271
BstMWI GCNNNNNNNGC 4 cut(s) 287, 847, 1010, 1329
BstNI CCWGG 2 cut(s) 417, 1245
BstNSI RCATGY 2 cut(s) 123, 251
BstSCI CCNGG 2 cut(s) 415, 1243
BstSFI CTRYAG 3 cut(s) 6, 1005, 1266
BstSLI GKGCMC 1 cut(s) 1559
BstUI CGCG 1 cut(s) 546
BstV1I GCAGC 4 cut(s) 479, 828, 946, 997
BstX2I RGATCY 1 cut(s) 1236
BstXI CCANNNNNNTGG 1 cut(s) 277
BstYI RGATCY 1 cut(s) 1236
BsuRI GGCC 3 cut(s) 325, 411, 850
BtgI CCRYGG 3 cut(s) 206, 484, 793
BtsCI GGATG 2 cut(s) 408, 1542
BtsIMutI CAGTG 3 cut(s) 179, 309, 844
BveI ACCTGC 1 cut(s) 710
Cac8I GCNNGC 2 cut(s) 717, 1002
CaiI CAGNNNCTG 1 cut(s) 1013
CciI TCATGA 1 cut(s) 616
CfoI GCGC 1 cut(s) 1064
Cfr13I GGNCC 2 cut(s) 323, 409
CsiI ACCWGGT 1 cut(s) 415
Csp6I GTAC 5 cut(s) 245, 889, 1083, 1128, 1389
CviAII CATG 9 cut(s) 120, 159, 248, 485, 533, 617, 794, 1328, 1486
CviQI GTAC 5 cut(s) 245, 889, 1083, 1128, 1389
DdeI CTNAG 8 cut(s) 430, 774, 810, 873, 879, 936, 1281, 1438
DpnI GATC 8 cut(s) 18, 49, 294, 615, 825, 1218, 1238, 1273
DpnII GATC 8 cut(s) 16, 47, 292, 613, 823, 1216, 1236, 1271
DraI TTTAAA 1 cut(s) 423
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Ecl136II GAGCTC 1 cut(s) 935
Eco130I CCWWGG 3 cut(s) 239, 484, 793
Eco24I GRGCYC 1 cut(s) 937
Eco53kI GAGCTC 1 cut(s) 935
Eco57I CTGAAG 2 cut(s) 584, 648
EcoICRI GAGCTC 1 cut(s) 935
EcoNI CCTNNNNNAGG 1 cut(s) 1550
EcoO109I RGGNCCY 1 cut(s) 323
EcoRI GAATTC 1 cut(s) 234
EcoRII CCWGG 2 cut(s) 415, 1243
EcoT14I CCWWGG 3 cut(s) 239, 484, 793
EcoT22I ATGCAT 1 cut(s) 1297
EcoT38I GRGCYC 1 cut(s) 937
ErhI CCWWGG 3 cut(s) 239, 484, 793
FaeI CATG 9 cut(s) 123, 162, 251, 488, 536, 620, 797, 1331, 1489
FatI CATG 9 cut(s) 119, 158, 247, 484, 532, 616, 793, 1327, 1485
FauI CCCGC 1 cut(s) 993
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 4 cut(s) 468, 842, 960, 1011
FokI GGATG 2 cut(s) 415, 1529
FriOI GRGCYC 1 cut(s) 937
Fsp4HI GCNGC 4 cut(s) 468, 842, 960, 1011
FspBI CTAG 3 cut(s) 240, 317, 1392
GlaI GCGC 1 cut(s) 1063
GluI GCNGC 4 cut(s) 468, 842, 960, 1011
GsaI CCCAGC 2 cut(s) 410, 1017
GsuI CTGGAG 1 cut(s) 203
HaeIII GGCC 3 cut(s) 325, 411, 850
HapII CCGG 1 cut(s) 285
HhaI GCGC 1 cut(s) 1064
Hin1II CATG 9 cut(s) 123, 162, 251, 488, 536, 620, 797, 1331, 1489
Hin6I GCGC 1 cut(s) 1062
HinP1I GCGC 1 cut(s) 1062
HincII GTYRAC 1 cut(s) 1287
HindII GTYRAC 1 cut(s) 1287
HindIII AAGCTT 3 cut(s) 145, 855, 1529
HpaII CCGG 1 cut(s) 285
HphI GGTGA 4 cut(s) 455, 1049, 1079, 1175
Hpy166II GTNNAC 1 cut(s) 1287
Hpy188I TCNGA 8 cut(s) 395, 438, 458, 874, 880, 939, 1345, 1419
Hpy188III TCNNGA 6 cut(s) 83, 450, 617, 707, 809, 994
Hpy8I GTNNAC 1 cut(s) 1287
HpyAV CCTTC 4 cut(s) 382, 815, 954, 1154
HpyCH4IV ACGT 1 cut(s) 952
HpyCH4V TGCA 9 cut(s) 60, 179, 539, 560, 629, 844, 984, 1295, 1599
HpyF10VI GCNNNNNNNGC 4 cut(s) 287, 847, 1010, 1329
HpyF3I CTNAG 8 cut(s) 430, 774, 810, 873, 879, 936, 1281, 1438
HpySE526I ACGT 1 cut(s) 952
Hsp92II CATG 9 cut(s) 123, 162, 251, 488, 536, 620, 797, 1331, 1489
HspAI GCGC 1 cut(s) 1062
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 8 cut(s) 16, 47, 292, 613, 823, 1216, 1236, 1271
LmnI GCTCC 1 cut(s) 922
Lsp1109I GCAGC 4 cut(s) 479, 828, 946, 997
LweI GCATC 6 cut(s) 31, 47, 393, 616, 907, 1282
MabI ACCWGGT 1 cut(s) 415
MaeI CTAG 3 cut(s) 240, 317, 1392
MaeII ACGT 1 cut(s) 952
MaeIII GTNAC 1 cut(s) 304
MalI GATC 8 cut(s) 18, 49, 294, 615, 825, 1218, 1238, 1273
MboI GATC 8 cut(s) 16, 47, 292, 613, 823, 1216, 1236, 1271
MboII GAAGA 6 cut(s) 371, 590, 982, 1082, 1211, 1588
MfeI CAATTG 2 cut(s) 1152, 1453
MflI RGATCY 1 cut(s) 1236
MhlI GDGCHC 3 cut(s) 937, 981, 1559
MlyI GAGTC 4 cut(s) 191, 705, 884, 1042
MmeI TCCRAC 1 cut(s) 533
Mph1103I ATGCAT 1 cut(s) 1297
MroXI GAANNNNTTC 1 cut(s) 1170
MseI TTAA 8 cut(s) 41, 422, 549, 735, 1110, 1191, 1314, 1606
MslI CAYNNNNRTG 2 cut(s) 275, 1332
MspA1I CMGCKG 2 cut(s) 604, 1010
MspI CCGG 1 cut(s) 285
MspR9I CCNGG 2 cut(s) 417, 1245
MunI CAATTG 2 cut(s) 1152, 1453
Mva1269I GAATGC 1 cut(s) 539
MvaI CCWGG 2 cut(s) 417, 1245
MvnI CGCG 1 cut(s) 546
MwoI GCNNNNNNNGC 4 cut(s) 287, 847, 1010, 1329
NcoI CCATGG 2 cut(s) 484, 793
NdeII GATC 8 cut(s) 16, 47, 292, 613, 823, 1216, 1236, 1271
NlaIII CATG 9 cut(s) 123, 162, 251, 488, 536, 620, 797, 1331, 1489
NlaIV GGNNCC 1 cut(s) 324
NmuCI GTSAC 1 cut(s) 304
NsiI ATGCAT 1 cut(s) 1297
NspI RCATGY 2 cut(s) 123, 251
PagI TCATGA 1 cut(s) 616
PctI GAATGC 1 cut(s) 539
PdmI GAANNNNTTC 1 cut(s) 1170
PfeI GAWTC 6 cut(s) 30, 446, 453, 492, 940, 1340
PfoI TCCNGGA 1 cut(s) 1243
PkrI GCNGC 4 cut(s) 469, 843, 961, 1012
PleI GAGTC 4 cut(s) 191, 704, 883, 1042
PpsI GAGTC 4 cut(s) 191, 704, 883, 1042
Ppu21I YACGTR 1 cut(s) 953
PshBI ATTAAT 1 cut(s) 1191
PsiI TTATAA 1 cut(s) 113
Psp124BI GAGCTC 1 cut(s) 937
Psp6I CCWGG 2 cut(s) 415, 1243
PspFI CCCAGC 2 cut(s) 406, 1013
PspGI CCWGG 2 cut(s) 415, 1243
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 2 cut(s) 323, 409
PstNI CAGNNNCTG 1 cut(s) 1013
PsuI RGATCY 1 cut(s) 1236
PvuII CAGCTG 1 cut(s) 1010
RsaI GTAC 5 cut(s) 246, 890, 1084, 1129, 1390
RsaNI GTAC 5 cut(s) 245, 889, 1083, 1128, 1389
RseI CAYNNNNRTG 2 cut(s) 275, 1332
SacI GAGCTC 1 cut(s) 937
SaqAI TTAA 8 cut(s) 41, 422, 549, 735, 1110, 1191, 1314, 1606
SatI GCNGC 4 cut(s) 468, 842, 960, 1011
Sau3AI GATC 8 cut(s) 16, 47, 292, 613, 823, 1216, 1236, 1271
Sau96I GGNCC 2 cut(s) 323, 409
ScaI AGTACT 1 cut(s) 1390
SchI GAGTC 4 cut(s) 191, 705, 884, 1042
ScrFI CCNGG 2 cut(s) 417, 1245
SduI GDGCHC 3 cut(s) 937, 981, 1559
SexAI ACCWGGT 1 cut(s) 415
SfaNI GCATC 6 cut(s) 31, 47, 393, 616, 907, 1282
SfcI CTRYAG 3 cut(s) 6, 1005, 1266
SmiMI CAYNNNNRTG 2 cut(s) 275, 1332
SmlI CTYRAG 3 cut(s) 852, 1550, 1567
SmoI CTYRAG 3 cut(s) 852, 1550, 1567
SpeI ACTAGT 1 cut(s) 1391
SsiI CCGC 6 cut(s) 123, 312, 544, 604, 920, 1000
SspI AATATT 2 cut(s) 136, 1170
SspMI CTAG 3 cut(s) 240, 317, 1392
SstI GAGCTC 1 cut(s) 937
StyD4I CCNGG 2 cut(s) 415, 1243
StyI CCWWGG 3 cut(s) 239, 484, 793
TaiI ACGT 1 cut(s) 955
TaqI TCGA 3 cut(s) 342, 1051, 1203
TaqII GACCGA 1 cut(s) 1304
TatI WGTACW 3 cut(s) 888, 1082, 1388
TfiI GAWTC 6 cut(s) 30, 446, 453, 492, 940, 1340
Tru1I TTAA 8 cut(s) 41, 422, 549, 735, 1110, 1191, 1314, 1606
Tru9I TTAA 8 cut(s) 41, 422, 549, 735, 1110, 1191, 1314, 1606
TscAI CASTG 3 cut(s) 186, 309, 851
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 4 cut(s) 467, 841, 959, 1010
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 6 cut(s) 372, 659, 1209, 1251, 1373, 1512
TspGWI ACGGA 3 cut(s) 42, 223, 956
TspRI CASTG 3 cut(s) 186, 309, 851
VspI ATTAAT 1 cut(s) 1191
XagI CCTNNNNNAGG 1 cut(s) 1550
XapI RAATTY 4 cut(s) 234, 525, 681, 1210
XceI RCATGY 2 cut(s) 123, 251
XcmI CCANNNNNNNNNTGG 1 cut(s) 834
XmaJI CCTAGG 1 cut(s) 239
XmnI GAANNNNTTC 1 cut(s) 1170
XspI CTAG 3 cut(s) 240, 317, 1392
ZrmI AGTACT 1 cut(s) 1390
Zsp2I ATGCAT 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.