Rw3G027650

Phosphatidylinositol 4-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0014385
Physical Location & Seq
Forward (+)
39677186 .. 39678832
1647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G027650.1

Sequence Viewer

Length: 1647 bp
ATGAGATTCGTCCAAATGGCTGTAGTAGTTGATCAACATCACGGAATCAAGCCATTAACCCGATCCCAGAGATGCAGATTACAATCCTACACCCATCTTGATTACAGCATATTTCCCCACTCCTTTATAAATACATGCGGAGTTGCTAATATTCACAAAAGCTTTTCCACCCCATGTCTCTCCCAAACTACTGCACTGGAGGACGACTTTGACTCTAATCCACGGATTGAGATTGTTGGTGGTTGCAGAGTTCCTAGGGTACATGCTCTTGTAGTTGAGGTTGCCATAGCTATGGCTTCCGGTGCTGATCTATTGCCAGTGCCAAGCGGACTAGGGGGGGCCTACTTCTTATGTAGTCGAAATGGTGACAATATTGCTGTAGCTAAGCCCATAGATGAAGAACCCTTAGCCTTCAATAATCCTAAAGGCTTCGGAGGTAGGATGCTGGGCCAACCTGGTTTAAAACGCTCAGTTCGGATTGGTGAATCTGGAATTCGAGAATTAGCAGCTTATCTCCTTGACCATGGTGGATTCGCTGGTGTTCCTCCAACTGCTTTAGTAAAAATTTCTCATGATGCATTCCGCGTTAATAATAATGCAGCTAAAATTTCAGCTACTCCGTACAAGATTGCTTCTCTTCAGCGGTTTGTAGATCATGACTTTGATGCAGGAGAGTTGGGTTTCTCTGGCTTCTCAATAGCTTCAGTTCATCACATTGGAATTTTTGATGTAAGAGTCCTAAATCTTGATAGGCACGCAGGTAATATGCTTGTTAAGAAGTATAAGCAGAACAACTATGCAGTTGGTGTGGCTGAGCTTGTGCCTATTGACCATGGGCTTTGCCTTCCTGAGTGGCTTGATGATCCATACTTTGAATGGCTGCACTGGCCTCAAGCTTCAATTCCATTTTCTGAGTCTGAGATTGAGTACATATCCAATCTCAATCCCTCTAAAGATGCGGAGCTTTTACGAACTCAGATTCCCTCTTTACGTGAGGCTGCTATCCGTGTTCTTGTGCTCTGCACCATCTTCTTGAAGCGGGCTACAGCTGCTGGGCTTTGTCTTGCTGATATAGGTGAAATGATGACTCGACAATTCTGTGCTGGCGAAGAAAGATTGAGTACATTAGAAAATCTTTGCTTACAAGTTAAAGCTACAATGTCTGGTACAGATGACAAAAACAGAGAAACAATTGGAGAAGGTGAAATATTTCAACTTCAGAATGATATTAAAGAAAGTTTCGATGAGAATTTTGATCTTCCTCAAGTCTTACAGATCTATCCTGGAATGTCAAGGTTTTCATCTGTAGGATCAATTACTGAGTTGACCGATGCATATATCTATCCACCATTTAGGGAAAGCAATCATGGCAATGATGATTCTGACAGTAAGAATATGAAAGGTAATAGTGGTTATGTTGATGGTAGTACTAGTGGTAGTCATAAGGTAGGCGGTCTGATGGAGAGAGTGAGTTTCTCAGTAAAAAACTTCAATTGTGAAACAGGGGCAATTTCTTTCGGAGACATGAGTAAAGATGAATGGGATTTGTTCCTGGACAATTTTGAGAAGCTTTTGCCCGACATCCTCCTCAAGGGCACAAATTACTTGAGCTTGAAGAAAAGGTTGAGAACTTCCTGCAAGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

548

Amino Acids

60.56

Weight (kDa)

5.76

Isoelectric Point (pI)

36.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 125 - 357 2.2e-41 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014385)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40850 AT3G56600 AT3G56600 AT3G56600
fragaria_vesca FvH4_1g29290
malus_domestica MD00G1140900.v1.1
prunus_persica Prupe.7G019100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0494791
rosa_laevigata RLG00000022662
rosa_multiflora Rmu_sc0000198.1_g000015
rosa_roxburghii Rroxscaffold_6G00390900
rosa_rugosa Rorug03G0268200
rosa_samantha Rh3AG317000 Rh3BG352200 Rh3CG349200 Rh3DG351600
rosa_wichuraiana Rw3G027650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 128
Acc36I ACCTGC 1 cut(s) 749
AccII CGCG 1 cut(s) 585
AciI CCGC 7 cut(s) 138, 327, 583, 643, 959, 1039, 1452
AclWI GGATC 3 cut(s) 57, 857, 1318
AcsI RAATTY 5 cut(s) 492, 564, 606, 720, 1249
AcuI CTGAAG 3 cut(s) 623, 687, 1202
AfaI GTAC 6 cut(s) 261, 623, 929, 1123, 1168, 1429
AfiI CCNNNNNNNGG 1 cut(s) 1591
AgsI TTSAA 7 cut(s) 415, 875, 900, 1036, 1214, 1492, 1615
AhlI ACTAGT 1 cut(s) 1430
AjnI CCWGG 3 cut(s) 454, 1282, 1551
AloI GAACNNNNNNTCC 2 cut(s) 964, 996
Alw21I GWGCWC 1 cut(s) 1020
Alw26I GTCTC 2 cut(s) 182, 1515
AlwI GGATC 3 cut(s) 57, 857, 1318
AlwNI CAGNNNCTG 1 cut(s) 1052
AoxI GGCC 3 cut(s) 339, 448, 887
ApeKI GCWGC 5 cut(s) 506, 599, 880, 998, 1049
ApoI RAATTY 5 cut(s) 492, 564, 606, 720, 1249
Asp700I GAANNNNTTC 1 cut(s) 1209
AspA2I CCTAGG 1 cut(s) 254
AspS9I GGNCC 2 cut(s) 339, 448
AsuHPI GGTGA 4 cut(s) 377, 494, 1088, 1214
AvrII CCTAGG 1 cut(s) 254
BaeGI GKGCMC 1 cut(s) 1598
Bbv12I GWGCWC 1 cut(s) 1020
BbvI GCAGC 5 cut(s) 518, 611, 867, 985, 1036
BccI CCATC 4 cut(s) 102, 1034, 1415, 1453
BciT130I CCWGG 3 cut(s) 456, 1284, 1553
BclI TGATCA 1 cut(s) 31
BcoDI GTCTC 2 cut(s) 182, 1515
BcuI ACTAGT 1 cut(s) 1430
BfaI CTAG 3 cut(s) 255, 332, 1431
BfmI CTRYAG 4 cut(s) 21, 378, 1044, 1305
BfuAI ACCTGC 1 cut(s) 749
BglII AGATCT 1 cut(s) 1275
BisI GCNGC 5 cut(s) 507, 600, 881, 999, 1050
BlnI CCTAGG 1 cut(s) 254
BlpI GCTNAGC 2 cut(s) 384, 813
BlsI GCNGC 5 cut(s) 508, 601, 882, 1000, 1051
BmcAI AGTACT 1 cut(s) 1429
Bme1390I CCNGG 3 cut(s) 456, 1284, 1553
BmgT120I GGNCC 2 cut(s) 339, 448
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 3 cut(s) 456, 1284, 1553
BmsI GCATC 6 cut(s) 62, 432, 565, 655, 946, 1321
BpmI CTGGAG 1 cut(s) 218
Bpu10I CCTNAGC 1 cut(s) 406
Bpu1102I GCTNAGC 2 cut(s) 384, 813
BpuEI CTTGAG 4 cut(s) 876, 1248, 1574, 1627
BsaAI YACGTR 1 cut(s) 992
BsaBI GATNNNNATC 2 cut(s) 36, 82
BsaJI CCNNGG 4 cut(s) 221, 254, 523, 832
BsaWI WCCGGW 1 cut(s) 299
Bsc4I CCNNNNNNNGG 1 cut(s) 1591
Bse1I ACTGG 3 cut(s) 201, 317, 890
Bse3DI GCAATG 1 cut(s) 1378
Bse8I GATNNNNATC 2 cut(s) 36, 82
BseBI CCWGG 3 cut(s) 456, 1284, 1553
BseDI CCNNGG 4 cut(s) 221, 254, 523, 832
BseGI GGATG 2 cut(s) 447, 1581
BseJI GATNNNNATC 2 cut(s) 36, 82
BseLI CCNNNNNNNGG 1 cut(s) 1591
BseMI GCAATG 1 cut(s) 1378
BseMII CTCAG 8 cut(s) 483, 804, 840, 903, 909, 989, 1311, 1491
BseNI ACTGG 3 cut(s) 201, 317, 890
BseRI GAGGAG 1 cut(s) 1577
BseSI GKGCMC 1 cut(s) 1598
BseXI GCAGC 5 cut(s) 518, 611, 867, 985, 1036
BseYI CCCAGC 2 cut(s) 445, 1052
BsgI GTGCAG 3 cut(s) 177, 866, 1006
Bsh1236I CGCG 1 cut(s) 585
BshFI GGCC 3 cut(s) 341, 450, 889
BsiHKAI GWGCWC 1 cut(s) 1020
BsiSI CCGG 1 cut(s) 300
BslI CCNNNNNNNGG 1 cut(s) 1591
BsmAI GTCTC 2 cut(s) 182, 1515
BsmI GAATGC 1 cut(s) 578
BsnI GGCC 3 cut(s) 341, 450, 889
Bsp1286I GDGCHC 2 cut(s) 1020, 1598
Bsp143I GATC 8 cut(s) 31, 62, 307, 652, 862, 1255, 1275, 1310
Bsp1720I GCTNAGC 2 cut(s) 384, 813
Bsp19I CCATGG 2 cut(s) 523, 832
BspACI CCGC 7 cut(s) 138, 327, 583, 643, 959, 1039, 1452
BspANI GGCC 3 cut(s) 341, 450, 889
BspCNI CTCAG 8 cut(s) 482, 805, 841, 904, 910, 988, 1312, 1490
BspFNI CGCG 1 cut(s) 585
BspHI TCATGA 2 cut(s) 571, 655
BspLI GGNNCC 1 cut(s) 340
BspMI ACCTGC 1 cut(s) 749
BspPI GGATC 3 cut(s) 57, 857, 1318
BsrDI GCAATG 1 cut(s) 1378
BsrI ACTGG 3 cut(s) 201, 317, 890
BssECI CCNNGG 4 cut(s) 221, 254, 523, 832
BssMI GATC 8 cut(s) 31, 62, 307, 652, 862, 1255, 1275, 1310
BssT1I CCWWGG 3 cut(s) 254, 523, 832
Bst2UI CCWGG 3 cut(s) 456, 1284, 1553
Bst4CI ACNGT 1 cut(s) 1388
Bst6I CTCTTC 1 cut(s) 642
BstBAI YACGTR 1 cut(s) 992
BstC8I GCNNGC 3 cut(s) 756, 1041, 1105
BstDSI CCRYGG 3 cut(s) 221, 523, 832
BstENI CCTNNNNNAGG 1 cut(s) 1589
BstF5I GGATG 2 cut(s) 447, 1581
BstFNI CGCG 1 cut(s) 585
BstKTI GATC 8 cut(s) 34, 65, 310, 655, 865, 1258, 1278, 1313
BstMAI GTCTC 2 cut(s) 182, 1515
BstMBI GATC 8 cut(s) 31, 62, 307, 652, 862, 1255, 1275, 1310
BstMWI GCNNNNNNNGC 4 cut(s) 302, 886, 1049, 1368
BstNI CCWGG 3 cut(s) 456, 1284, 1553
BstNSI RCATGY 2 cut(s) 138, 266
BstSCI CCNGG 3 cut(s) 454, 1282, 1551
BstSFI CTRYAG 4 cut(s) 21, 378, 1044, 1305
BstSLI GKGCMC 1 cut(s) 1598
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 5 cut(s) 518, 611, 867, 985, 1036
BstX2I RGATCY 1 cut(s) 1275
BstXI CCANNNNNNTGG 1 cut(s) 292
BstYI RGATCY 1 cut(s) 1275
BsuRI GGCC 3 cut(s) 341, 450, 889
BtgI CCRYGG 3 cut(s) 221, 523, 832
BtsCI GGATG 2 cut(s) 447, 1581
BtsIMutI CAGTG 3 cut(s) 194, 324, 883
BveI ACCTGC 1 cut(s) 749
Cac8I GCNNGC 3 cut(s) 756, 1041, 1105
CaiI CAGNNNCTG 1 cut(s) 1052
CciI TCATGA 2 cut(s) 571, 655
Cfr13I GGNCC 2 cut(s) 339, 448
CsiI ACCWGGT 1 cut(s) 454
Csp6I GTAC 6 cut(s) 260, 622, 928, 1122, 1167, 1428
CviAII CATG 9 cut(s) 135, 174, 263, 524, 572, 656, 833, 1367, 1525
CviQI GTAC 6 cut(s) 260, 622, 928, 1122, 1167, 1428
DpnI GATC 8 cut(s) 33, 64, 309, 654, 864, 1257, 1277, 1312
DpnII GATC 8 cut(s) 31, 62, 307, 652, 862, 1255, 1275, 1310
DraI TTTAAA 1 cut(s) 462
Eam1104I CTCTTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 642
Eco130I CCWWGG 3 cut(s) 254, 523, 832
Eco57I CTGAAG 3 cut(s) 623, 687, 1202
EcoNI CCTNNNNNAGG 1 cut(s) 1589
EcoO109I RGGNCCY 1 cut(s) 339
EcoRI GAATTC 1 cut(s) 492
EcoRII CCWGG 3 cut(s) 454, 1282, 1551
EcoT14I CCWWGG 3 cut(s) 254, 523, 832
EcoT22I ATGCAT 2 cut(s) 580, 1336
ErhI CCWWGG 3 cut(s) 254, 523, 832
FaeI CATG 9 cut(s) 138, 177, 266, 527, 575, 659, 836, 1370, 1528
FatI CATG 9 cut(s) 134, 173, 262, 523, 571, 655, 832, 1366, 1524
FauI CCCGC 1 cut(s) 1032
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 5 cut(s) 507, 600, 881, 999, 1050
FokI GGATG 2 cut(s) 454, 1568
Fsp4HI GCNGC 5 cut(s) 507, 600, 881, 999, 1050
FspBI CTAG 3 cut(s) 255, 332, 1431
GluI GCNGC 5 cut(s) 507, 600, 881, 999, 1050
GsaI CCCAGC 2 cut(s) 449, 1056
GsuI CTGGAG 1 cut(s) 218
HaeIII GGCC 3 cut(s) 341, 450, 889
HapII CCGG 1 cut(s) 300
Hin1II CATG 9 cut(s) 138, 177, 266, 527, 575, 659, 836, 1370, 1528
HincII GTYRAC 1 cut(s) 1326
HindII GTYRAC 1 cut(s) 1326
HindIII AAGCTT 3 cut(s) 160, 894, 1568
HpaII CCGG 1 cut(s) 300
HphI GGTGA 4 cut(s) 377, 494, 1088, 1214
Hpy166II GTNNAC 1 cut(s) 1326
Hpy188I TCNGA 9 cut(s) 434, 477, 913, 919, 978, 1221, 1384, 1458, 1520
Hpy188III TCNNGA 8 cut(s) 98, 489, 497, 572, 656, 746, 848, 1033
Hpy8I GTNNAC 1 cut(s) 1326
HpyAV CCTTC 3 cut(s) 421, 854, 1193
HpyCH4III ACNGT 1 cut(s) 1388
HpyCH4IV ACGT 1 cut(s) 991
HpyF10VI GCNNNNNNNGC 4 cut(s) 302, 886, 1049, 1368
HpySE526I ACGT 1 cut(s) 991
Hsp92II CATG 9 cut(s) 138, 177, 266, 527, 575, 659, 836, 1370, 1528
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 8 cut(s) 31, 62, 307, 652, 862, 1255, 1275, 1310
LmnI GCTCC 1 cut(s) 961
Lsp1109I GCAGC 5 cut(s) 518, 611, 867, 985, 1036
LweI GCATC 6 cut(s) 62, 432, 565, 655, 946, 1321
MabI ACCWGGT 1 cut(s) 454
MaeI CTAG 3 cut(s) 255, 332, 1431
MaeII ACGT 1 cut(s) 991
MaeIII GTNAC 1 cut(s) 365
MalI GATC 8 cut(s) 33, 64, 309, 654, 864, 1257, 1277, 1312
MboI GATC 8 cut(s) 31, 62, 307, 652, 862, 1255, 1275, 1310
MboII GAAGA 6 cut(s) 410, 629, 1021, 1121, 1250, 1627
MfeI CAATTG 2 cut(s) 1191, 1492
MflI RGATCY 1 cut(s) 1275
MhlI GDGCHC 2 cut(s) 1020, 1598
MlyI GAGTC 4 cut(s) 206, 744, 923, 1081
MmeI TCCRAC 1 cut(s) 572
Mph1103I ATGCAT 2 cut(s) 580, 1336
MroXI GAANNNNTTC 1 cut(s) 1209
MseI TTAA 7 cut(s) 56, 461, 588, 774, 1149, 1230, 1645
MslI CAYNNNNRTG 2 cut(s) 290, 1371
MspA1I CMGCKG 2 cut(s) 643, 1049
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 3 cut(s) 456, 1284, 1553
MunI CAATTG 2 cut(s) 1191, 1492
Mva1269I GAATGC 1 cut(s) 578
MvaI CCWGG 3 cut(s) 456, 1284, 1553
MvnI CGCG 1 cut(s) 585
MwoI GCNNNNNNNGC 4 cut(s) 302, 886, 1049, 1368
NcoI CCATGG 2 cut(s) 523, 832
NdeII GATC 8 cut(s) 31, 62, 307, 652, 862, 1255, 1275, 1310
NlaIII CATG 9 cut(s) 138, 177, 266, 527, 575, 659, 836, 1370, 1528
NlaIV GGNNCC 1 cut(s) 340
NmuCI GTSAC 1 cut(s) 365
NsiI ATGCAT 2 cut(s) 580, 1336
NspI RCATGY 2 cut(s) 138, 266
PagI TCATGA 2 cut(s) 571, 655
PctI GAATGC 1 cut(s) 578
PdmI GAANNNNTTC 1 cut(s) 1209
PfeI GAWTC 6 cut(s) 6, 45, 485, 531, 979, 1379
PfoI TCCNGGA 2 cut(s) 1282, 1551
PkrI GCNGC 5 cut(s) 508, 601, 882, 1000, 1051
PleI GAGTC 4 cut(s) 206, 743, 922, 1081
PpsI GAGTC 4 cut(s) 206, 743, 922, 1081
Ppu21I YACGTR 1 cut(s) 992
PsiI TTATAA 1 cut(s) 128
Psp6I CCWGG 3 cut(s) 454, 1282, 1551
PspFI CCCAGC 2 cut(s) 445, 1052
PspGI CCWGG 3 cut(s) 454, 1282, 1551
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 2 cut(s) 339, 448
PstNI CAGNNNCTG 1 cut(s) 1052
PsuI RGATCY 1 cut(s) 1275
PvuII CAGCTG 1 cut(s) 1049
RsaI GTAC 6 cut(s) 261, 623, 929, 1123, 1168, 1429
RsaNI GTAC 6 cut(s) 260, 622, 928, 1122, 1167, 1428
RseI CAYNNNNRTG 2 cut(s) 290, 1371
SaqAI TTAA 7 cut(s) 56, 461, 588, 774, 1149, 1230, 1645
SatI GCNGC 5 cut(s) 507, 600, 881, 999, 1050
Sau3AI GATC 8 cut(s) 31, 62, 307, 652, 862, 1255, 1275, 1310
Sau96I GGNCC 2 cut(s) 339, 448
ScaI AGTACT 1 cut(s) 1429
SchI GAGTC 4 cut(s) 206, 744, 923, 1081
ScrFI CCNGG 3 cut(s) 456, 1284, 1553
SduI GDGCHC 2 cut(s) 1020, 1598
SexAI ACCWGGT 1 cut(s) 454
SfaNI GCATC 6 cut(s) 62, 432, 565, 655, 946, 1321
SfcI CTRYAG 4 cut(s) 21, 378, 1044, 1305
SmiMI CAYNNNNRTG 2 cut(s) 290, 1371
SmlI CTYRAG 4 cut(s) 891, 1263, 1589, 1606
SmoI CTYRAG 4 cut(s) 891, 1263, 1589, 1606
SpeI ACTAGT 1 cut(s) 1430
SsiI CCGC 7 cut(s) 138, 327, 583, 643, 959, 1039, 1452
SspI AATATT 3 cut(s) 151, 373, 1209
SspMI CTAG 3 cut(s) 255, 332, 1431
StyD4I CCNGG 3 cut(s) 454, 1282, 1551
StyI CCWWGG 3 cut(s) 254, 523, 832
TaaI ACNGT 1 cut(s) 1388
TaiI ACGT 1 cut(s) 994
TaqI TCGA 4 cut(s) 358, 496, 1090, 1242
TaqII GACCGA 1 cut(s) 1343
TatI WGTACW 3 cut(s) 927, 1121, 1427
TfiI GAWTC 6 cut(s) 6, 45, 485, 531, 979, 1379
Tru1I TTAA 7 cut(s) 56, 461, 588, 774, 1149, 1230, 1645
Tru9I TTAA 7 cut(s) 56, 461, 588, 774, 1149, 1230, 1645
TscAI CASTG 3 cut(s) 201, 324, 890
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 5 cut(s) 506, 599, 880, 998, 1049
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 5 cut(s) 411, 698, 1290, 1412, 1551
TspGWI ACGGA 4 cut(s) 57, 238, 609, 995
TspRI CASTG 3 cut(s) 201, 324, 890
XagI CCTNNNNNAGG 1 cut(s) 1589
XapI RAATTY 5 cut(s) 492, 564, 606, 720, 1249
XceI RCATGY 2 cut(s) 138, 266
XcmI CCANNNNNNNNNTGG 1 cut(s) 873
XmaJI CCTAGG 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 1209
XspI CTAG 3 cut(s) 255, 332, 1431
ZrmI AGTACT 1 cut(s) 1429
Zsp2I ATGCAT 2 cut(s) 580, 1336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.