RLG00000022662

Phosphatidylinositol 4-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0014385
Physical Location & Seq
Reverse (-)
6970636 .. 6972264
1629 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022662

Sequence Viewer

Length: 1629 bp
ATGGCTGTAGCAATTGATCAACATCACGGAATCAAGCCATTAAGCCGATCCCAGAGATGCAGATTACAATCCTACACCCATCTTGATTACAGCATATTTCCCCACTCCTTTATAAATACATGCGGAGTTGCTAATATTCACAAAAGCTTTTCCACCCCATGTCTCTCCCAAACTACTGCACTGGAGGACGACTTTGACTCTAATCCACGGATTGAGATTGTTGGTGGTAGCAGAATTCCTAGGGTACATGCTCTTGTAGTTGAGGTTGCCATAGCTATGGCTTCCGGTGCTGATCTATTGCCAGTGCCAAGCGGACTAGGGGGGGCCTACTTCTTATGTAGTCGAAATGGTGACAATATTGCTGTAGCTAAGCCCATAGATGAAGAACCCTTAGCCTTCAATAATCCTAAAGGCTTCGGAGGTAGGATGCTGGGCCAACCTGGTTTAAAACGCTCAGTTCGGATTGGTGAATCTGGAATCCGAGAATTAGCAGCTTATCTCCTTGACCATGGTGGATTCGCTGGTGTTCCTCCAACTGCTTTAGTAAAAATTTCTCATGTTGCATTCCGCGTTAATAATAATGCACCTACAATTTCTGCTACCCCCTACAAGATTGCTTCTCTTCAGCGGTTTGTAGATCATGACTTTGATGCAGGAGAGTTGGGTTTCTCTGGCTTCTCAATAGCTTCAGTTCATCACATTGGAATTTTTGATGTAAGAGTCCTAAATCTTGATAGGCACGCAGGTAATATGCTTGTTAAGTATAAGCAGAACAACTATGCAGTTGGTGTGGCTGAGCTTGTGCCTATTGACCATGGGCTTTGCCTTCCTGAGTGGCTTGATGATCCATACTTTGAATGGCTGCACTGGCCTCAAGCTTCAATTCCATTTTCTGAGTCTGAGATTGAGTACATATCCAATCTCAATCCCTCTAAAGATGCGGAGCTTTTACGAACTCAGATTCCTTCTTTACGTGAGGCTGCTATCCGTGTTCTTGTGCTCTGCACCATCTTCTTGAAGCGGGCTACAGCTGCTGGGCTTTGTCTTGCTGATATAGGTGAAATGATGACTCGACAATTCTGCACTGGTGAAGAAAGATTGAGTACATTAGAAAATCTTTGCTTACAAGTTAAAGCTACAATGTCTGGTACAGATGACAAAAACAGAGAAACAATTGGAGAAGGTGAAATATTTCAACTTCACAATGATATTAAAGAAAGTTTCGATGAGAATTTTGATCTTCCTCAACTCTTACAGATCTATCCTGGAATGTCAAGGTTTTCATCTGTAGGATCAATTACTGAGTTGACCGATGCATATATCTATCCACCATTTAAGGAAAGCAATCATGGCAATGATGATTCTGATAGTAAGAATATGAAAGGTAATAGCGGTTATGTTGATGGTAGTACTAGTGGTAGTCATAAGGTAGGGGGTCTGATGGAGAGAGTGAATTTCTCAGTAAATAACTTCAACTGTGAAACAGGGGCAATTTCTTTCGGAGACATGAGTAAAGATGAATGGGATCTGTTCTTGGACAATTTTGAGAAGCTTTTGCCAGACATCCTCCTCAAGGGCACAAATTACTTGAGCTTGAAGAAAAGGTTGAGAACTTCCTGCAAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

543

Amino Acids

59.75

Weight (kDa)

5.6

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 120 - 351 9.7e-42 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014385)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40850 AT3G56600 AT3G56600 AT3G56600
fragaria_vesca FvH4_1g29290
malus_domestica MD00G1140900.v1.1
prunus_persica Prupe.7G019100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0494791
rosa_laevigata RLG00000022662
rosa_multiflora Rmu_sc0000198.1_g000015
rosa_roxburghii Rroxscaffold_6G00390900
rosa_rugosa Rorug03G0268200
rosa_samantha Rh3AG317000 Rh3BG352200 Rh3CG349200 Rh3DG351600
rosa_wichuraiana Rw3G027650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 113
Acc36I ACCTGC 1 cut(s) 734
AccII CGCG 1 cut(s) 570
AciI CCGC 7 cut(s) 123, 312, 568, 628, 941, 1021, 1392
AclWI GGATC 4 cut(s) 42, 839, 1300, 1533
AcsI RAATTY 5 cut(s) 234, 549, 705, 1231, 1453
AcuI CTGAAG 2 cut(s) 608, 672
AfaI GTAC 5 cut(s) 246, 911, 1105, 1150, 1411
AfiI CCNNNNNNNGG 1 cut(s) 1573
AgsI TTSAA 7 cut(s) 400, 857, 882, 1018, 1196, 1474, 1597
AhlI ACTAGT 1 cut(s) 1412
AjnI CCWGG 2 cut(s) 439, 1264
AloI GAACNNNNNNTCC 2 cut(s) 946, 978
Alw21I GWGCWC 1 cut(s) 1002
Alw26I GTCTC 2 cut(s) 167, 1497
AlwI GGATC 4 cut(s) 42, 839, 1300, 1533
AlwNI CAGNNNCTG 1 cut(s) 1034
AoxI GGCC 3 cut(s) 324, 433, 869
ApeKI GCWGC 4 cut(s) 491, 862, 980, 1031
ApoI RAATTY 5 cut(s) 234, 549, 705, 1231, 1453
Asp700I GAANNNNTTC 1 cut(s) 1191
AspA2I CCTAGG 1 cut(s) 239
AspS9I GGNCC 2 cut(s) 324, 433
AsuHPI GGTGA 5 cut(s) 362, 479, 1070, 1100, 1196
AvrII CCTAGG 1 cut(s) 239
BaeGI GKGCMC 1 cut(s) 1580
Bbv12I GWGCWC 1 cut(s) 1002
BbvI GCAGC 4 cut(s) 503, 849, 967, 1018
BccI CCATC 4 cut(s) 87, 1016, 1397, 1435
BcgI CGANNNNNNTGC 2 cut(s) 1062, 1096
BciT130I CCWGG 2 cut(s) 441, 1266
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 2 cut(s) 167, 1497
BcuI ACTAGT 1 cut(s) 1412
BfaI CTAG 3 cut(s) 240, 317, 1413
BfmI CTRYAG 4 cut(s) 6, 363, 1026, 1287
BfuAI ACCTGC 1 cut(s) 734
BglII AGATCT 1 cut(s) 1257
BisI GCNGC 4 cut(s) 492, 863, 981, 1032
BlnI CCTAGG 1 cut(s) 239
BlpI GCTNAGC 2 cut(s) 369, 795
BlsI GCNGC 4 cut(s) 493, 864, 982, 1033
BmcAI AGTACT 1 cut(s) 1411
Bme1390I CCNGG 2 cut(s) 441, 1266
BmgT120I GGNCC 2 cut(s) 324, 433
BmiI GGNNCC 1 cut(s) 325
BmrFI CCNGG 2 cut(s) 441, 1266
BmsI GCATC 5 cut(s) 47, 417, 640, 928, 1303
BpmI CTGGAG 1 cut(s) 203
Bpu10I CCTNAGC 1 cut(s) 391
Bpu1102I GCTNAGC 2 cut(s) 369, 795
BpuEI CTTGAG 3 cut(s) 858, 1556, 1609
BsaAI YACGTR 1 cut(s) 974
BsaBI GATNNNNATC 2 cut(s) 21, 67
BsaJI CCNNGG 4 cut(s) 206, 239, 508, 814
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 1 cut(s) 1573
Bse1I ACTGG 4 cut(s) 186, 302, 872, 1090
Bse3DI GCAATG 1 cut(s) 1360
Bse8I GATNNNNATC 2 cut(s) 21, 67
BseBI CCWGG 2 cut(s) 441, 1266
BseDI CCNNGG 4 cut(s) 206, 239, 508, 814
BseGI GGATG 2 cut(s) 432, 1563
BseJI GATNNNNATC 2 cut(s) 21, 67
BseLI CCNNNNNNNGG 1 cut(s) 1573
BseMI GCAATG 1 cut(s) 1360
BseMII CTCAG 8 cut(s) 468, 786, 822, 885, 891, 971, 1293, 1473
BseNI ACTGG 4 cut(s) 186, 302, 872, 1090
BseRI GAGGAG 1 cut(s) 1559
BseSI GKGCMC 1 cut(s) 1580
BseXI GCAGC 4 cut(s) 503, 849, 967, 1018
BseYI CCCAGC 2 cut(s) 430, 1034
BsgI GTGCAG 4 cut(s) 162, 848, 988, 1066
Bsh1236I CGCG 1 cut(s) 570
BshFI GGCC 3 cut(s) 326, 435, 871
BsiHKAI GWGCWC 1 cut(s) 1002
BsiSI CCGG 1 cut(s) 285
BslI CCNNNNNNNGG 1 cut(s) 1573
BsmAI GTCTC 2 cut(s) 167, 1497
BsmI GAATGC 1 cut(s) 563
BsnI GGCC 3 cut(s) 326, 435, 871
Bsp1286I GDGCHC 2 cut(s) 1002, 1580
Bsp143I GATC 9 cut(s) 16, 47, 292, 637, 844, 1237, 1257, 1292, 1525
Bsp1720I GCTNAGC 2 cut(s) 369, 795
Bsp19I CCATGG 2 cut(s) 508, 814
BspACI CCGC 7 cut(s) 123, 312, 568, 628, 941, 1021, 1392
BspANI GGCC 3 cut(s) 326, 435, 871
BspCNI CTCAG 8 cut(s) 467, 787, 823, 886, 892, 970, 1294, 1472
BspFNI CGCG 1 cut(s) 570
BspHI TCATGA 1 cut(s) 640
BspLI GGNNCC 1 cut(s) 325
BspMI ACCTGC 1 cut(s) 734
BspPI GGATC 4 cut(s) 42, 839, 1300, 1533
BsrDI GCAATG 1 cut(s) 1360
BsrI ACTGG 4 cut(s) 186, 302, 872, 1090
BssECI CCNNGG 4 cut(s) 206, 239, 508, 814
BssMI GATC 9 cut(s) 16, 47, 292, 637, 844, 1237, 1257, 1292, 1525
BssT1I CCWWGG 3 cut(s) 239, 508, 814
Bst2UI CCWGG 2 cut(s) 441, 1266
Bst4CI ACNGT 1 cut(s) 1478
Bst6I CTCTTC 1 cut(s) 627
BstBAI YACGTR 1 cut(s) 974
BstC8I GCNNGC 2 cut(s) 741, 1023
BstDSI CCRYGG 3 cut(s) 206, 508, 814
BstENI CCTNNNNNAGG 1 cut(s) 1571
BstF5I GGATG 2 cut(s) 432, 1563
BstFNI CGCG 1 cut(s) 570
BstKTI GATC 9 cut(s) 19, 50, 295, 640, 847, 1240, 1260, 1295, 1528
BstMAI GTCTC 2 cut(s) 167, 1497
BstMBI GATC 9 cut(s) 16, 47, 292, 637, 844, 1237, 1257, 1292, 1525
BstMWI GCNNNNNNNGC 4 cut(s) 287, 868, 1031, 1350
BstNI CCWGG 2 cut(s) 441, 1266
BstNSI RCATGY 2 cut(s) 123, 251
BstSCI CCNGG 2 cut(s) 439, 1264
BstSFI CTRYAG 4 cut(s) 6, 363, 1026, 1287
BstSLI GKGCMC 1 cut(s) 1580
BstUI CGCG 1 cut(s) 570
BstV1I GCAGC 4 cut(s) 503, 849, 967, 1018
BstX2I RGATCY 2 cut(s) 1257, 1525
BstXI CCANNNNNNTGG 1 cut(s) 277
BstYI RGATCY 2 cut(s) 1257, 1525
BsuRI GGCC 3 cut(s) 326, 435, 871
BtgI CCRYGG 3 cut(s) 206, 508, 814
BtsCI GGATG 2 cut(s) 432, 1563
BtsIMutI CAGTG 4 cut(s) 179, 309, 865, 1083
BveI ACCTGC 1 cut(s) 734
Cac8I GCNNGC 2 cut(s) 741, 1023
CaiI CAGNNNCTG 1 cut(s) 1034
CciI TCATGA 1 cut(s) 640
Cfr13I GGNCC 2 cut(s) 324, 433
CsiI ACCWGGT 1 cut(s) 439
Csp6I GTAC 5 cut(s) 245, 910, 1104, 1149, 1410
CviAII CATG 9 cut(s) 120, 159, 248, 509, 557, 641, 815, 1349, 1507
CviQI GTAC 5 cut(s) 245, 910, 1104, 1149, 1410
DpnI GATC 9 cut(s) 18, 49, 294, 639, 846, 1239, 1259, 1294, 1527
DpnII GATC 9 cut(s) 16, 47, 292, 637, 844, 1237, 1257, 1292, 1525
DraI TTTAAA 1 cut(s) 447
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
Eco130I CCWWGG 3 cut(s) 239, 508, 814
Eco57I CTGAAG 2 cut(s) 608, 672
EcoNI CCTNNNNNAGG 1 cut(s) 1571
EcoO109I RGGNCCY 1 cut(s) 324
EcoRI GAATTC 1 cut(s) 234
EcoRII CCWGG 2 cut(s) 439, 1264
EcoT14I CCWWGG 3 cut(s) 239, 508, 814
EcoT22I ATGCAT 1 cut(s) 1318
ErhI CCWWGG 3 cut(s) 239, 508, 814
FaeI CATG 9 cut(s) 123, 162, 251, 512, 560, 644, 818, 1352, 1510
FatI CATG 9 cut(s) 119, 158, 247, 508, 556, 640, 814, 1348, 1506
FauI CCCGC 1 cut(s) 1014
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 4 cut(s) 492, 863, 981, 1032
FokI GGATG 2 cut(s) 439, 1550
Fsp4HI GCNGC 4 cut(s) 492, 863, 981, 1032
FspBI CTAG 3 cut(s) 240, 317, 1413
GluI GCNGC 4 cut(s) 492, 863, 981, 1032
GsaI CCCAGC 2 cut(s) 434, 1038
GsuI CTGGAG 1 cut(s) 203
HaeIII GGCC 3 cut(s) 326, 435, 871
HapII CCGG 1 cut(s) 285
Hin1II CATG 9 cut(s) 123, 162, 251, 512, 560, 644, 818, 1352, 1510
HincII GTYRAC 1 cut(s) 1308
HindII GTYRAC 1 cut(s) 1308
HindIII AAGCTT 3 cut(s) 145, 876, 1550
HpaII CCGG 1 cut(s) 285
HphI GGTGA 5 cut(s) 362, 479, 1070, 1100, 1196
Hpy166II GTNNAC 1 cut(s) 1308
Hpy188I TCNGA 9 cut(s) 419, 462, 482, 895, 901, 960, 1366, 1440, 1502
Hpy188III TCNNGA 6 cut(s) 83, 474, 641, 731, 830, 1015
Hpy8I GTNNAC 1 cut(s) 1308
HpyAV CCTTC 4 cut(s) 406, 836, 975, 1175
HpyCH4III ACNGT 1 cut(s) 1478
HpyCH4IV ACGT 1 cut(s) 973
HpyF10VI GCNNNNNNNGC 4 cut(s) 287, 868, 1031, 1350
HpySE526I ACGT 1 cut(s) 973
Hsp92II CATG 9 cut(s) 123, 162, 251, 512, 560, 644, 818, 1352, 1510
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 9 cut(s) 16, 47, 292, 637, 844, 1237, 1257, 1292, 1525
LmnI GCTCC 1 cut(s) 943
Lsp1109I GCAGC 4 cut(s) 503, 849, 967, 1018
LweI GCATC 5 cut(s) 47, 417, 640, 928, 1303
MabI ACCWGGT 1 cut(s) 439
MaeI CTAG 3 cut(s) 240, 317, 1413
MaeII ACGT 1 cut(s) 973
MaeIII GTNAC 1 cut(s) 350
MalI GATC 9 cut(s) 18, 49, 294, 639, 846, 1239, 1259, 1294, 1527
MboI GATC 9 cut(s) 16, 47, 292, 637, 844, 1237, 1257, 1292, 1525
MboII GAAGA 6 cut(s) 395, 614, 1003, 1103, 1232, 1609
MfeI CAATTG 2 cut(s) 12, 1173
MflI RGATCY 2 cut(s) 1257, 1525
MhlI GDGCHC 2 cut(s) 1002, 1580
MlyI GAGTC 4 cut(s) 191, 729, 905, 1063
MmeI TCCRAC 1 cut(s) 557
Mph1103I ATGCAT 1 cut(s) 1318
MroXI GAANNNNTTC 1 cut(s) 1191
MseI TTAA 8 cut(s) 41, 446, 573, 759, 1131, 1212, 1335, 1627
MslI CAYNNNNRTG 2 cut(s) 275, 1353
MspA1I CMGCKG 2 cut(s) 628, 1031
MspI CCGG 1 cut(s) 285
MspR9I CCNGG 2 cut(s) 441, 1266
MunI CAATTG 2 cut(s) 12, 1173
Mva1269I GAATGC 1 cut(s) 563
MvaI CCWGG 2 cut(s) 441, 1266
MvnI CGCG 1 cut(s) 570
MwoI GCNNNNNNNGC 4 cut(s) 287, 868, 1031, 1350
NcoI CCATGG 2 cut(s) 508, 814
NdeII GATC 9 cut(s) 16, 47, 292, 637, 844, 1237, 1257, 1292, 1525
NlaIII CATG 9 cut(s) 123, 162, 251, 512, 560, 644, 818, 1352, 1510
NlaIV GGNNCC 1 cut(s) 325
NmuCI GTSAC 1 cut(s) 350
NsiI ATGCAT 1 cut(s) 1318
NspI RCATGY 2 cut(s) 123, 251
PagI TCATGA 1 cut(s) 640
PctI GAATGC 1 cut(s) 563
PdmI GAANNNNTTC 1 cut(s) 1191
PfeI GAWTC 6 cut(s) 30, 470, 477, 516, 961, 1361
PfoI TCCNGGA 1 cut(s) 1264
PkrI GCNGC 4 cut(s) 493, 864, 982, 1033
PleI GAGTC 4 cut(s) 191, 728, 904, 1063
PpsI GAGTC 4 cut(s) 191, 728, 904, 1063
Ppu21I YACGTR 1 cut(s) 974
PsiI TTATAA 1 cut(s) 113
Psp6I CCWGG 2 cut(s) 439, 1264
PspFI CCCAGC 2 cut(s) 430, 1034
PspGI CCWGG 2 cut(s) 439, 1264
PspN4I GGNNCC 1 cut(s) 325
PspPI GGNCC 2 cut(s) 324, 433
PstNI CAGNNNCTG 1 cut(s) 1034
PsuI RGATCY 2 cut(s) 1257, 1525
PvuII CAGCTG 1 cut(s) 1031
RsaI GTAC 5 cut(s) 246, 911, 1105, 1150, 1411
RsaNI GTAC 5 cut(s) 245, 910, 1104, 1149, 1410
RseI CAYNNNNRTG 2 cut(s) 275, 1353
SaqAI TTAA 8 cut(s) 41, 446, 573, 759, 1131, 1212, 1335, 1627
SatI GCNGC 4 cut(s) 492, 863, 981, 1032
Sau3AI GATC 9 cut(s) 16, 47, 292, 637, 844, 1237, 1257, 1292, 1525
Sau96I GGNCC 2 cut(s) 324, 433
ScaI AGTACT 1 cut(s) 1411
SchI GAGTC 4 cut(s) 191, 729, 905, 1063
ScrFI CCNGG 2 cut(s) 441, 1266
SduI GDGCHC 2 cut(s) 1002, 1580
SexAI ACCWGGT 1 cut(s) 439
SfaNI GCATC 5 cut(s) 47, 417, 640, 928, 1303
SfcI CTRYAG 4 cut(s) 6, 363, 1026, 1287
SmiMI CAYNNNNRTG 2 cut(s) 275, 1353
SmlI CTYRAG 3 cut(s) 873, 1571, 1588
SmoI CTYRAG 3 cut(s) 873, 1571, 1588
SpeI ACTAGT 1 cut(s) 1412
SsiI CCGC 7 cut(s) 123, 312, 568, 628, 941, 1021, 1392
SspI AATATT 3 cut(s) 136, 358, 1191
SspMI CTAG 3 cut(s) 240, 317, 1413
StyD4I CCNGG 2 cut(s) 439, 1264
StyI CCWWGG 3 cut(s) 239, 508, 814
TaaI ACNGT 1 cut(s) 1478
TaiI ACGT 1 cut(s) 976
TaqI TCGA 3 cut(s) 343, 1072, 1224
TaqII GACCGA 1 cut(s) 1325
TatI WGTACW 3 cut(s) 909, 1103, 1409
TfiI GAWTC 6 cut(s) 30, 470, 477, 516, 961, 1361
Tru1I TTAA 8 cut(s) 41, 446, 573, 759, 1131, 1212, 1335, 1627
Tru9I TTAA 8 cut(s) 41, 446, 573, 759, 1131, 1212, 1335, 1627
TscAI CASTG 4 cut(s) 186, 309, 872, 1090
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 4 cut(s) 491, 862, 980, 1031
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 5 cut(s) 396, 683, 1272, 1394, 1533
TspGWI ACGGA 3 cut(s) 42, 223, 977
TspRI CASTG 4 cut(s) 186, 309, 872, 1090
XagI CCTNNNNNAGG 1 cut(s) 1571
XapI RAATTY 5 cut(s) 234, 549, 705, 1231, 1453
XceI RCATGY 2 cut(s) 123, 251
XcmI CCANNNNNNNNNTGG 1 cut(s) 855
XmaJI CCTAGG 1 cut(s) 239
XmnI GAANNNNTTC 1 cut(s) 1191
XspI CTAG 3 cut(s) 240, 317, 1413
ZrmI AGTACT 1 cut(s) 1411
Zsp2I ATGCAT 1 cut(s) 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.