MD00G1140900.v1.1

Phosphatidylinositol 4-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0014385
Physical Location & Seq
Forward (+)
30792539 .. 30796488
3950 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1140900.v1.1.491

Sequence Viewer

Length: 1653 bp
ATGAGAATTGTCAAAATGGCTGTAGCAGTTGATCAACATCACGGATTCAAGACATTTAGCCGATCCCAGAGATGCAGATTACAGTCCTACACTCAACTTGATTATAACATTCTTGCCCACTCCTTAAAACATGCATTCGGTGATGCTAATTTTCATAAAAGCTTCTCCACCCCATGTCTATCCCTAACCACTGCATCGGAGGAAGATTTTGACACTAATCCAAGAATTGAGATTGTTGGTGGCAGTAGGATACCTAGGGTACATGCTCTTGTAGTCGAGGTTGCCATATCTATGGCGTCTGGTGCCGACCTCCTGCCAGTGTCAAGTGGACTAGGCGGTGCCTACTACTTATGTAGCAAAAATGGCGACAATATTGCTGTGGCAAAGCCCATAGATGAAGAACCTTTAGCTTTCAATAATCCTAAGGGTCTCGGAGGCAGGATGCTGGGCCAACCTGGTTTGAAACGCTCAGTTCGGATTGGTGAATCTGGAATCCGAGAATTGGCAGCATATCTCCTTGACCACGGTGGATTCGCTGGTGTTCCTCCAACAGCTTTAGTGAAAATTTCTCATGTTGCATTCCACATTAACAAAAATGCAGGCGCAATTTCAGCTTCACCCTATAAGATTGCTTCTCTGCAGCGCTTCGTGGATCATGACTTTGATGCTGGAGAGTTAGGTTCTTCAGGCTTTTCAGTAGCTGCTGTCCATCGTATTGGAATTTTTGATGTAAGACTCCTGAATCTTGACAGGCATGCAGGGAACATGCTTGTTAAGAAATATGAGCAGCACAACTATGCAGCTGGGGTGCTTGAGCTTGTACCTATTGACCATGGTCTTTGCCTTCCTGAGTGGCTTGATGATCCATATTTCGAATGGTTGCACTGGCCTCAAGCTTCAGTTCCTTTTTCCGAGTCTGAAATCGAGTACATATCCAAACTTGACCCCTACAAAGATGCGGAGCTTCTACGAACTCAGATTCCTTCTTTCCGGGAGGCTGCTATTCGTGTTCTTGTGCTATGCACCATCTTCTTGAAGCAGTCTGCTGCTTCAGGGCTCTGTCTTGCTGATATAGGAGAAATGATGACTCGGCAGTTCTGTGCCGACGAAGAAACATTGAGTACATTGGAGAATTTGTGCATGCAAGTTAAGAATGCCATGCCTACTAGAGACAACGAGAAGAAAGGAGAAATCGGAGAAGTTGAAATATTTGAATTTGACAATGAAAATAAAGAAGGGTTTAATGAGAATATGGATCTTCCGAGCCATCCCGAAATTATTATGTCAAGGTTTTTATCAGCGGGATCAATGACTGGGTTGGCCGATGCATCTCTGTCTCCTCTATTCGAGGAAAATGATCAAACCATTGACAACTATGATAGAAACACTATAACAGGCAGCAACAATGTTGGTGATGATGGAACTACTAGTGATGGTCGCGGTCGTAAGGTAGGGGTTTTGATGAAGAGCGGGAGTTTCTCAGCAGCGACCCTCAGTTGTGAAACTCGGAGAGCAATATCATTTGGAGACATGAGTGGAGACGAATGGGAATTGTTCTTGGACCATTTTGACAAGTTTTTGGCGCACAAACTGGAGGAGGGCAACAAGTGCTTGAGCTCGAAACTGAGGTTGGGAACTTCCTGCAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

551

Amino Acids

60.46

Weight (kDa)

5.38

Isoelectric Point (pI)

39.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 125 - 357 4e-41 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014385)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40850 AT3G56600 AT3G56600 AT3G56600
fragaria_vesca FvH4_1g29290
malus_domestica MD00G1140900.v1.1
prunus_persica Prupe.7G019100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0494791
rosa_laevigata RLG00000022662
rosa_multiflora Rmu_sc0000198.1_g000015
rosa_roxburghii Rroxscaffold_6G00390900
rosa_rugosa Rorug03G0268200
rosa_samantha Rh3AG317000 Rh3BG352200 Rh3CG349200 Rh3DG351600
rosa_wichuraiana Rw3G027650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 105
AccB1I GGYRCC 2 cut(s) 302, 338
AccBSI CCGCTC 1 cut(s) 1470
AccII CGCG 1 cut(s) 1440
AciI CCGC 5 cut(s) 336, 959, 1301, 1440, 1470
AclWI GGATC 5 cut(s) 57, 660, 857, 1263, 1312
AcoI YGGCCR 1 cut(s) 1320
AcsI RAATTY 4 cut(s) 564, 720, 1132, 1214
AcuI CTGAAG 3 cut(s) 669, 882, 1035
AcyI GRCGYC 1 cut(s) 296
AfaI GTAC 4 cut(s) 261, 822, 929, 1123
AfeI AGCGCT 1 cut(s) 644
AfiI CCNNNNNNNGG 1 cut(s) 502
AgsI TTSAA 6 cut(s) 49, 415, 463, 1036, 1205, 1214
AhlI ACTAGT 1 cut(s) 1427
AjnI CCWGG 1 cut(s) 454
AjuI GAANNNNNNNTTGG 2 cut(s) 1613, 1645
AloI GAACNNNNNNTCC 2 cut(s) 964, 996
Alw21I GWGCWC 1 cut(s) 1619
Alw26I GTCTC 5 cut(s) 434, 1164, 1341, 1521, 1533
AlwI GGATC 5 cut(s) 57, 660, 857, 1263, 1312
AlwNI CAGNNNCTG 1 cut(s) 701
Aor51HI AGCGCT 1 cut(s) 644
AoxI GGCC 3 cut(s) 448, 887, 1320
ApeKI GCWGC 9 cut(s) 506, 640, 701, 787, 800, 998, 1046, 1398, 1484
ApoI RAATTY 4 cut(s) 564, 720, 1132, 1214
AspA2I CCTAGG 1 cut(s) 254
AspLEI GCGC 3 cut(s) 605, 645, 1585
AspS9I GGNCC 2 cut(s) 448, 1561
AsuC2I CCSGG 1 cut(s) 992
AsuHPI GGTGA 4 cut(s) 152, 494, 609, 1424
AsuII TTCGAA 1 cut(s) 873
AvaII GGWCC 1 cut(s) 1561
AvrII CCTAGG 1 cut(s) 254
AxyI CCTNAGG 1 cut(s) 423
BanI GGYRCC 2 cut(s) 302, 338
BanII GRGCYC 2 cut(s) 1059, 1619
Bbv12I GWGCWC 1 cut(s) 1619
BbvI GCAGC 9 cut(s) 518, 652, 688, 799, 812, 985, 1033, 1410, 1496
BccI CCATC 5 cut(s) 717, 1034, 1275, 1412, 1427
BcgI CGANNNNNNTGC 2 cut(s) 356, 390
BciT130I CCWGG 1 cut(s) 456
BciVI GTATCC 1 cut(s) 243
BclI TGATCA 2 cut(s) 31, 1357
BcnI CCSGG 1 cut(s) 992
BcoDI GTCTC 5 cut(s) 434, 1164, 1341, 1521, 1533
BcuI ACTAGT 1 cut(s) 1427
BfaI CTAG 4 cut(s) 255, 332, 1167, 1428
BfmI CTRYAG 2 cut(s) 21, 638
BfoI RGCGCY 1 cut(s) 646
BfuI GTATCC 1 cut(s) 243
BisI GCNGC 9 cut(s) 507, 641, 702, 788, 801, 999, 1047, 1399, 1485
BlnI CCTAGG 1 cut(s) 254
BlsI GCNGC 9 cut(s) 508, 642, 703, 789, 802, 1000, 1048, 1400, 1486
Bme1390I CCNGG 2 cut(s) 456, 992
Bme18I GGWCC 1 cut(s) 1561
BmgT120I GGNCC 2 cut(s) 448, 1561
BmiI GGNNCC 2 cut(s) 304, 340
BmrFI CCNGG 2 cut(s) 456, 992
BmrI ACTGGG 1 cut(s) 1323
BmsI GCATC 8 cut(s) 62, 133, 203, 432, 655, 946, 1315, 1337
BmuI ACTGGG 1 cut(s) 1323
BoxI GACNNNNGTC 1 cut(s) 834
BpmI CTGGAG 2 cut(s) 690, 1613
Bpu14I TTCGAA 1 cut(s) 873
BpuEI CTTGAG 3 cut(s) 833, 876, 1633
BpuMI CCSGG 1 cut(s) 992
BsaBI GATNNNNATC 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 3 cut(s) 254, 523, 832
BsaXI ACNNNNNCTCC 2 cut(s) 1121, 1151
Bsc4I CCNNNNNNNGG 1 cut(s) 502
Bse1I ACTGG 4 cut(s) 317, 890, 1318, 1596
Bse21I CCTNAGG 1 cut(s) 423
Bse8I GATNNNNATC 1 cut(s) 36
BseBI CCWGG 1 cut(s) 456
BseDI CCNNGG 3 cut(s) 254, 523, 832
BseGI GGATG 2 cut(s) 447, 1267
BseJI GATNNNNATC 1 cut(s) 36
BseLI CCNNNNNNNGG 1 cut(s) 502
BseMII CTCAG 6 cut(s) 483, 840, 989, 1494, 1507, 1616
BseNI ACTGG 4 cut(s) 317, 890, 1318, 1596
BseRI GAGGAG 2 cut(s) 1329, 1610
BseXI GCAGC 9 cut(s) 518, 652, 688, 799, 812, 985, 1033, 1410, 1496
BseYI CCCAGC 2 cut(s) 445, 803
Bsh1236I CGCG 1 cut(s) 1440
Bsh1285I CGRYCG 1 cut(s) 1444
BshFI GGCC 3 cut(s) 450, 889, 1322
BshNI GGYRCC 2 cut(s) 302, 338
BsiEI CGRYCG 1 cut(s) 1444
BsiHKAI GWGCWC 1 cut(s) 1619
BsiSI CCGG 1 cut(s) 991
BslI CCNNNNNNNGG 1 cut(s) 502
BsmAI GTCTC 5 cut(s) 434, 1164, 1341, 1521, 1533
BsmBI CGTCTC 1 cut(s) 1533
BsmI GAATGC 3 cut(s) 134, 578, 1159
BsnI GGCC 3 cut(s) 450, 889, 1322
Bso31I GGTCTC 1 cut(s) 434
Bsp119I TTCGAA 1 cut(s) 873
Bsp1286I GDGCHC 2 cut(s) 1059, 1619
Bsp143I GATC 7 cut(s) 31, 62, 652, 862, 1255, 1304, 1357
Bsp19I CCATGG 1 cut(s) 832
BspACI CCGC 5 cut(s) 336, 959, 1301, 1440, 1470
BspANI GGCC 3 cut(s) 450, 889, 1322
BspCNI CTCAG 6 cut(s) 482, 841, 988, 1493, 1506, 1617
BspFNI CGCG 1 cut(s) 1440
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 2 cut(s) 304, 340
BspMAI CTGCAG 1 cut(s) 642
BspPI GGATC 5 cut(s) 57, 660, 857, 1263, 1312
BspQI GCTCTTC 1 cut(s) 1460
BspT104I TTCGAA 1 cut(s) 873
BspT107I GGYRCC 2 cut(s) 302, 338
BspTNI GGTCTC 1 cut(s) 434
BsrBI CCGCTC 1 cut(s) 1470
BsrI ACTGG 4 cut(s) 317, 890, 1318, 1596
BssECI CCNNGG 3 cut(s) 254, 523, 832
BssMI GATC 7 cut(s) 31, 62, 652, 862, 1255, 1304, 1357
BssNI GRCGYC 1 cut(s) 296
BssT1I CCWWGG 2 cut(s) 254, 832
Bst2UI CCWGG 1 cut(s) 456
Bst4CI ACNGT 2 cut(s) 84, 527
Bst6I CTCTTC 1 cut(s) 1460
BstACI GRCGYC 1 cut(s) 296
BstAPI GCANNNNNTGC 1 cut(s) 1608
BstBI TTCGAA 1 cut(s) 873
BstC8I GCNNGC 3 cut(s) 601, 756, 1142
BstDEI CTNAG 7 cut(s) 423, 469, 849, 975, 1480, 1493, 1625
BstDSI CCRYGG 2 cut(s) 523, 832
BstF5I GGATG 2 cut(s) 447, 1267
BstFNI CGCG 1 cut(s) 1440
BstH2I RGCGCY 1 cut(s) 646
BstHHI GCGC 3 cut(s) 605, 645, 1585
BstKTI GATC 7 cut(s) 34, 65, 655, 865, 1258, 1307, 1360
BstMAI GTCTC 5 cut(s) 434, 1164, 1341, 1521, 1533
BstMBI GATC 7 cut(s) 31, 62, 652, 862, 1255, 1304, 1357
BstMCI CGRYCG 1 cut(s) 1444
BstMWI GCNNNNNNNGC 4 cut(s) 302, 363, 611, 1608
BstNI CCWGG 1 cut(s) 456
BstNSI RCATGY 5 cut(s) 134, 266, 758, 769, 1144
BstPAI GACNNNNGTC 1 cut(s) 834
BstSCI CCNGG 2 cut(s) 454, 990
BstSFI CTRYAG 2 cut(s) 21, 638
BstUI CGCG 1 cut(s) 1440
BstV1I GCAGC 9 cut(s) 518, 652, 688, 799, 812, 985, 1033, 1410, 1496
BstX2I RGATCY 1 cut(s) 1255
BstXI CCANNNNNNTGG 2 cut(s) 292, 716
BstYI RGATCY 1 cut(s) 1255
Bsu36I CCTNAGG 1 cut(s) 423
BsuI GTATCC 1 cut(s) 243
BsuRI GGCC 3 cut(s) 450, 889, 1322
BtgI CCRYGG 2 cut(s) 523, 832
BtsCI GGATG 2 cut(s) 447, 1267
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 3 cut(s) 189, 324, 883
Cac8I GCNNGC 3 cut(s) 601, 756, 1142
CaiI CAGNNNCTG 1 cut(s) 701
CciI TCATGA 1 cut(s) 655
CfoI GCGC 3 cut(s) 605, 645, 1585
Cfr13I GGNCC 2 cut(s) 448, 1561
CseI GACGC 1 cut(s) 285
CsiI ACCWGGT 1 cut(s) 454
Csp6I GTAC 4 cut(s) 260, 821, 928, 1122
CviQI GTAC 4 cut(s) 260, 821, 928, 1122
DdeI CTNAG 7 cut(s) 423, 469, 849, 975, 1480, 1493, 1625
DpnI GATC 7 cut(s) 33, 64, 654, 864, 1257, 1306, 1359
DpnII GATC 7 cut(s) 31, 62, 652, 862, 1255, 1304, 1357
EaeI YGGCCR 1 cut(s) 1320
Eam1104I CTCTTC 1 cut(s) 1460
EarI CTCTTC 1 cut(s) 1460
Ecl136II GAGCTC 1 cut(s) 1617
Eco130I CCWWGG 2 cut(s) 254, 832
Eco24I GRGCYC 2 cut(s) 1059, 1619
Eco31I GGTCTC 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 1561
Eco47III AGCGCT 1 cut(s) 644
Eco53kI GAGCTC 1 cut(s) 1617
Eco57I CTGAAG 3 cut(s) 669, 882, 1035
Eco81I CCTNAGG 1 cut(s) 423
EcoICRI GAGCTC 1 cut(s) 1617
EcoRII CCWGG 1 cut(s) 454
EcoT14I CCWWGG 2 cut(s) 254, 832
EcoT22I ATGCAT 2 cut(s) 136, 1330
EcoT38I GRGCYC 2 cut(s) 1059, 1619
ErhI CCWWGG 2 cut(s) 254, 832
Esp3I CGTCTC 1 cut(s) 1533
FauI CCCGC 2 cut(s) 1294, 1463
FbaI TGATCA 2 cut(s) 31, 1357
Fnu4HI GCNGC 9 cut(s) 507, 641, 702, 788, 801, 999, 1047, 1399, 1485
FokI GGATG 2 cut(s) 454, 1254
FriOI GRGCYC 2 cut(s) 1059, 1619
Fsp4HI GCNGC 9 cut(s) 507, 641, 702, 788, 801, 999, 1047, 1399, 1485
FspBI CTAG 4 cut(s) 255, 332, 1167, 1428
GlaI GCGC 3 cut(s) 604, 644, 1584
GluI GCNGC 9 cut(s) 507, 641, 702, 788, 801, 999, 1047, 1399, 1485
GsaI CCCAGC 2 cut(s) 449, 807
GsuI CTGGAG 2 cut(s) 690, 1613
HaeII RGCGCY 1 cut(s) 646
HaeIII GGCC 3 cut(s) 450, 889, 1322
HapII CCGG 1 cut(s) 991
HgaI GACGC 1 cut(s) 285
HhaI GCGC 3 cut(s) 605, 645, 1585
Hin1I GRCGYC 1 cut(s) 296
Hin6I GCGC 3 cut(s) 603, 643, 1583
HinP1I GCGC 3 cut(s) 603, 643, 1583
HindIII AAGCTT 2 cut(s) 160, 894
HinfI GANTC 9 cut(s) 45, 485, 492, 531, 735, 742, 914, 979, 1087
HpaII CCGG 1 cut(s) 991
HphI GGTGA 4 cut(s) 152, 494, 609, 1424
Hpy166II GTNNAC 1 cut(s) 329
Hpy188III TCNNGA 8 cut(s) 49, 489, 656, 739, 746, 848, 1033, 1271
Hpy8I GTNNAC 1 cut(s) 329
Hpy99I CGWCG 1 cut(s) 1109
HpyAV CCTTC 3 cut(s) 854, 993, 1229
HpyCH4III ACNGT 2 cut(s) 84, 527
HpyF10VI GCNNNNNNNGC 4 cut(s) 302, 363, 611, 1608
HpyF3I CTNAG 7 cut(s) 423, 469, 849, 975, 1480, 1493, 1625
Hsp92I GRCGYC 1 cut(s) 296
HspAI GCGC 3 cut(s) 603, 643, 1583
Ksp22I TGATCA 2 cut(s) 31, 1357
Kzo9I GATC 7 cut(s) 31, 62, 652, 862, 1255, 1304, 1357
LguI GCTCTTC 1 cut(s) 1460
LmnI GCTCC 1 cut(s) 961
Lsp1109I GCAGC 9 cut(s) 518, 652, 688, 799, 812, 985, 1033, 1410, 1496
LweI GCATC 8 cut(s) 62, 133, 203, 432, 655, 946, 1315, 1337
MabI ACCWGGT 1 cut(s) 454
MaeI CTAG 4 cut(s) 255, 332, 1167, 1428
MalI GATC 7 cut(s) 33, 64, 654, 864, 1257, 1306, 1359
MbiI CCGCTC 1 cut(s) 1470
MboI GATC 7 cut(s) 31, 62, 652, 862, 1255, 1304, 1357
MboII GAAGA 8 cut(s) 215, 410, 675, 1021, 1121, 1192, 1250, 1477
MflI RGATCY 1 cut(s) 1255
MhlI GDGCHC 2 cut(s) 1059, 1619
MlyI GAGTC 3 cut(s) 729, 923, 1081
MmeI TCCRAC 1 cut(s) 572
Mph1103I ATGCAT 2 cut(s) 136, 1330
MseI TTAA 5 cut(s) 125, 588, 774, 1149, 1242
MslI CAYNNNNRTG 2 cut(s) 290, 795
MspA1I CMGCKG 2 cut(s) 803, 1301
MspI CCGG 1 cut(s) 991
MspR9I CCNGG 2 cut(s) 456, 992
Mva1269I GAATGC 3 cut(s) 134, 578, 1159
MvaI CCWGG 1 cut(s) 456
MvnI CGCG 1 cut(s) 1440
MwoI GCNNNNNNNGC 4 cut(s) 302, 363, 611, 1608
NciI CCSGG 1 cut(s) 992
NcoI CCATGG 1 cut(s) 832
NdeII GATC 7 cut(s) 31, 62, 652, 862, 1255, 1304, 1357
NlaIV GGNNCC 2 cut(s) 304, 340
NmeAIII GCCGAG 1 cut(s) 1069
NsiI ATGCAT 2 cut(s) 136, 1330
NspI RCATGY 5 cut(s) 134, 266, 758, 769, 1144
NspV TTCGAA 1 cut(s) 873
PaeI GCATGC 2 cut(s) 758, 1144
PagI TCATGA 1 cut(s) 655
PciSI GCTCTTC 1 cut(s) 1460
PctI GAATGC 3 cut(s) 134, 578, 1159
PfeI GAWTC 6 cut(s) 45, 485, 492, 531, 742, 979
PfoI TCCNGGA 1 cut(s) 990
PkrI GCNGC 9 cut(s) 508, 642, 703, 789, 802, 1000, 1048, 1400, 1486
PleI GAGTC 3 cut(s) 729, 922, 1081
PpsI GAGTC 3 cut(s) 729, 922, 1081
PshAI GACNNNNGTC 1 cut(s) 834
PsiI TTATAA 1 cut(s) 105
Psp124BI GAGCTC 1 cut(s) 1619
Psp6I CCWGG 1 cut(s) 454
PspFI CCCAGC 2 cut(s) 445, 803
PspGI CCWGG 1 cut(s) 454
PspN4I GGNNCC 2 cut(s) 304, 340
PspPI GGNCC 2 cut(s) 448, 1561
PstI CTGCAG 1 cut(s) 642
PstNI CAGNNNCTG 1 cut(s) 701
PsuI RGATCY 1 cut(s) 1255
PvuII CAGCTG 1 cut(s) 803
RsaI GTAC 4 cut(s) 261, 822, 929, 1123
RsaNI GTAC 4 cut(s) 260, 821, 928, 1122
RseI CAYNNNNRTG 2 cut(s) 290, 795
SacI GAGCTC 1 cut(s) 1619
SapI GCTCTTC 1 cut(s) 1460
SaqAI TTAA 5 cut(s) 125, 588, 774, 1149, 1242
SatI GCNGC 9 cut(s) 507, 641, 702, 788, 801, 999, 1047, 1399, 1485
Sau3AI GATC 7 cut(s) 31, 62, 652, 862, 1255, 1304, 1357
Sau96I GGNCC 2 cut(s) 448, 1561
SchI GAGTC 3 cut(s) 729, 923, 1081
ScrFI CCNGG 2 cut(s) 456, 992
SduI GDGCHC 2 cut(s) 1059, 1619
SexAI ACCWGGT 1 cut(s) 454
SfaNI GCATC 8 cut(s) 62, 133, 203, 432, 655, 946, 1315, 1337
SfcI CTRYAG 2 cut(s) 21, 638
SfuI TTCGAA 1 cut(s) 873
SinI GGWCC 1 cut(s) 1561
SmiMI CAYNNNNRTG 2 cut(s) 290, 795
SmlI CTYRAG 3 cut(s) 812, 891, 1612
SmoI CTYRAG 3 cut(s) 812, 891, 1612
SpeI ACTAGT 1 cut(s) 1427
SphI GCATGC 2 cut(s) 758, 1144
SsiI CCGC 5 cut(s) 336, 959, 1301, 1440, 1470
SspI AATATT 2 cut(s) 373, 1209
SspMI CTAG 4 cut(s) 255, 332, 1167, 1428
SstI GAGCTC 1 cut(s) 1619
StyD4I CCNGG 2 cut(s) 454, 990
StyI CCWWGG 2 cut(s) 254, 832
TaaI ACNGT 2 cut(s) 84, 527
TaqI TCGA 5 cut(s) 276, 873, 924, 1347, 1619
TatI WGTACW 2 cut(s) 927, 1121
TfiI GAWTC 6 cut(s) 45, 485, 492, 531, 742, 979
Tru1I TTAA 5 cut(s) 125, 588, 774, 1149, 1242
Tru9I TTAA 5 cut(s) 125, 588, 774, 1149, 1242
TscAI CASTG 3 cut(s) 196, 324, 890
TseI GCWGC 9 cut(s) 506, 640, 701, 787, 800, 998, 1046, 1398, 1484
TspDTI ATGAA 4 cut(s) 143, 411, 1239, 1478
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 3 cut(s) 196, 324, 890
VpaK11BI GGWCC 1 cut(s) 1561
XapI RAATTY 4 cut(s) 564, 720, 1132, 1214
XceI RCATGY 5 cut(s) 134, 266, 758, 769, 1144
XcmI CCANNNNNNNNNTGG 1 cut(s) 873
XmaJI CCTAGG 1 cut(s) 254
XspI CTAG 4 cut(s) 255, 332, 1167, 1428
Zsp2I ATGCAT 2 cut(s) 136, 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.