FvH4_2g03320

zinc metallopeptidase EGY3, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016229
Physical Location & Seq
Reverse (-)
2594610 .. 2598525
3916 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g03320.t1

Sequence Viewer

Length: 1698 bp
ATGGCTTCTCTTTCTTTCACTTCAGCTTCCTCCATACCTTCATGTCCTCCCATGAACAAGAACACCAAAACCCCATTTGGTAAAAGAACCCATTACTTCTCTTTTACTTCATCTCCTAAACCCATCTCAAGAAAACACCACCCCATTACATTTTCCATGAAAGATGACCAATCTTCTTCTTCTTCTTCAACTGCTGTAGTTTCTGAGAAGCCAAGTGATGACACTAGTGATACCCAAAAGAGTGGAGAAGAAGGCGAAAACGGTGAAGAGGGTGATGAGGAGAAGCAAGAAATGGATTGGAAGACAGATGAGGAGTTCAAGAGGTTCATGGGGAACCCCTCAATTGAAGCTGCAATAAAGTTGGAGAAGAAGAGGGCAGATAGGAAGCTCAAGGATCTTGATAGGGAAAGTAGTGATAACCCAATTGTAGGGCTGCTTAATAGAGCTTTAAGAGATAGTTTGACTAGAGAGAAGGAGAGATTGGAGAAAGCTGAAGAGACCTTCAAGGCTATTGACCTTAACAAGTTGAAGAGTTGTTTTGGTTTCGATACGTTTTTTCCAACTGATGTTCGGAGATTTGGAGATGGAGGCATTTTCATTGGAAATTTGAGGAGACCAATTGAAGAGGTCATGCCCAAATTAGAGAAAAAGCTGTCTGAAGCGGCAGGAAGAGAGGTTGTTTTATGGTTCATGGAGGAAAAAACAAATGACATCACAAAACAGGTCTGTATGGTGCAACCTAAAGCAGAAATAGATCTCCAATTTGAGTATACCAAGCTGAGCACTCCGTGGGGTTATGTTAGCGCAGTAGCATTGTGTGTTGCTACTTTTGGAACAATAGCTCTGATGAGTGGCTTCTTCCTTAAACCTGGTGCTACTTTTGATGACTACGTAGCCAATGCTGTGCCTCTCTTCAGTGGCTTCATATCCATTTTGGGAGTTTCTGAGATAGCCACTAGAGTAACAGCAGCTCGGTATGGTGTAAAACTCAGCCCATCTTTTCTTGTGCCATCCAATTGGACGGGGTGTGTAGGAGTGATGAATAACTATGAATCACTTCTTCCAAATAAAAAAGCTCTCTTTGATATTCCAGTGGCGCGCACAGCTAGTGCATATATTACATCTCTGATGCTTGCAGTTGCTGCATTTGTGGTGGATGGCAGCTTCAACGGGGGTGACAATGCATTGTATATAAGGCCTCAGTTCTTTGACAACAACCCACTGCTTTCTTTTATCCAATTCGTTATTGGTCCTTATGCTGATGATCTTGGAAATGTGCTACCCAATGCAGTGGAAGGTGTTGGAGTTCCTGTTGATCCCCTTGCTTTTGCTGGACTTCTAGGAATGGTTGTGACATCTCTGAACTTGTTGCCTTGTGGAAGGCTCGAGGGAGGCCGGATTGCACAAGCTATGTTTGGGAGAAGCACAGCTACTCTGCTATCATTTGCCACATCGCTTTTACTTGGCATTGGTGGCCTGAGTGGAAGTGTCCTTTGCTTGGCATGGGGGTTATTTGCAACCTTCTTCCGAGGTGGAGAAGAAGTACCTGCAAAAGATGAGATCACTCCATTAGGGGATGACAGATTTGCTTGGGGTTGTGTCCTTGGACTCATATGTTTCCTCACTCTATTCCCCAATGGCGGAGGAACATTCTCCAGCTCATTTTTCAATGCGCCGTTCTTCAGGGGAGATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0006950 GO:0006979 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0010035 GO:0042221 GO:0042493 GO:0042542 GO:0046677 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

566

Amino Acids

61.62

Weight (kDa)

5.26

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016229)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17870
fragaria_vesca FvH4_2g03320
malus_domestica MD10G1110200.v1.1
prunus_persica Prupe.8G150400_v2.0.a1
pyrus_communis pycom10g09570
rosa_chinensis RchiOBHm_Chr6g0248441
rosa_laevigata RLG00000015153
rosa_roxburghii Rroxscaffold_7G00213280
rosa_rugosa Rorug05G0531100 Rorug05G0531200
rosa_samantha Rh6AG047000 Rh6BG043600 Rh6CG040300 Rh6DG036800
rosa_wichuraiana Rw6G004100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1555
AccI GTMKAC 1 cut(s) 770
AccII CGCG 1 cut(s) 1099
AciI CCGC 2 cut(s) 662, 1641
AclWI GGATC 2 cut(s) 402, 1310
AcsI RAATTY 1 cut(s) 604
AcuI CTGAAG 5 cut(s) 6, 513, 678, 898, 1666
AdeI CACNNNGTG 1 cut(s) 789
AfaI GTAC 1 cut(s) 1545
AfiI CCNNNNNNNGG 3 cut(s) 428, 1498, 1640
AgsI TTSAA 8 cut(s) 189, 319, 347, 505, 529, 623, 1168, 1669
AhlI ACTAGT 1 cut(s) 224
AjnI CCWGG 1 cut(s) 868
AjuI GAANNNNNNNTTGG 2 cut(s) 464, 496
Alw21I GWGCWC 1 cut(s) 785
Alw26I GTCTC 2 cut(s) 491, 607
AlwI GGATC 2 cut(s) 402, 1310
AlwNI CAGNNNCTG 1 cut(s) 1142
Ama87I CYCGRG 1 cut(s) 1385
AoxI GGCC 3 cut(s) 1196, 1393, 1474
ApeKI GCWGC 5 cut(s) 350, 433, 968, 1142, 1161
ApoI RAATTY 1 cut(s) 604
Asp700I GAANNNNTTC 1 cut(s) 1056
AspLEI GCGC 4 cut(s) 806, 1099, 1101, 1675
AspS9I GGNCC 1 cut(s) 1250
AsuHPI GGTGA 3 cut(s) 275, 284, 1187
AvaI CYCGRG 1 cut(s) 1385
AvaII GGWCC 1 cut(s) 1250
BarI GAAGNNNNNNTAC 2 cut(s) 1414, 1446
BbsI GAAGAC 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 785
BbvI GCAGC 5 cut(s) 337, 420, 980, 1129, 1173
BccI CCATC 5 cut(s) 131, 578, 1003, 1018, 1151
BceAI ACGGC 1 cut(s) 1660
BciT130I CCWGG 1 cut(s) 870
BcoDI GTCTC 2 cut(s) 491, 607
BcuI ACTAGT 1 cut(s) 224
BfaI CTAG 5 cut(s) 225, 465, 957, 1107, 1340
BfmI CTRYAG 1 cut(s) 195
BfuAI ACCTGC 1 cut(s) 1555
BglII AGATCT 2 cut(s) 754, 1690
BisI GCNGC 6 cut(s) 351, 434, 663, 969, 1143, 1162
BlpI GCTNAGC 1 cut(s) 779
BlsI GCNGC 6 cut(s) 352, 435, 664, 970, 1144, 1163
Bme1390I CCNGG 1 cut(s) 870
Bme18I GGWCC 1 cut(s) 1250
BmeT110I CYCGRG 1 cut(s) 1385
BmgT120I GGNCC 1 cut(s) 1250
BmiI GGNNCC 1 cut(s) 335
BmrFI CCNGG 1 cut(s) 870
BmsI GCATC 1 cut(s) 1119
BpiI GAAGAC 1 cut(s) 308
BpmI CTGGAG 1 cut(s) 1639
Bpu1102I GCTNAGC 1 cut(s) 779
BpuEI CTTGAG 2 cut(s) 112, 374
BsaAI YACGTR 1 cut(s) 892
BsaI GGTCTC 2 cut(s) 491, 607
BsaJI CCNNGG 3 cut(s) 788, 1528, 1603
BsaXI ACNNNNNCTCC 6 cut(s) 97, 127, 1296, 1326, 1527, 1557
Bsc4I CCNNNNNNNGG 3 cut(s) 428, 1498, 1640
Bse1I ACTGG 1 cut(s) 1091
BseBI CCWGG 1 cut(s) 870
BseDI CCNNGG 3 cut(s) 788, 1528, 1603
BseGI GGATG 3 cut(s) 1010, 1162, 1582
BseLI CCNNNNNNNGG 3 cut(s) 428, 1498, 1640
BseMII CTCAG 6 cut(s) 195, 770, 936, 1003, 1214, 1469
BseNI ACTGG 1 cut(s) 1091
BsePI GCGCGC 1 cut(s) 1097
BseRI GAGGAG 3 cut(s) 293, 326, 625
BseXI GCAGC 5 cut(s) 337, 420, 980, 1129, 1173
Bsh1236I CGCG 1 cut(s) 1099
BshFI GGCC 3 cut(s) 1198, 1395, 1476
BsiHKAI GWGCWC 1 cut(s) 785
BsiHKCI CYCGRG 1 cut(s) 1385
BsiSI CCGG 1 cut(s) 1396
BslI CCNNNNNNNGG 3 cut(s) 428, 1498, 1640
BsmAI GTCTC 2 cut(s) 491, 607
BsnI GGCC 3 cut(s) 1198, 1395, 1476
Bso31I GGTCTC 2 cut(s) 491, 607
BsoBI CYCGRG 1 cut(s) 1385
Bsp1286I GDGCHC 1 cut(s) 785
Bsp143I GATC 6 cut(s) 394, 754, 1264, 1315, 1560, 1690
Bsp1720I GCTNAGC 1 cut(s) 779
BspACI CCGC 2 cut(s) 662, 1641
BspANI GGCC 3 cut(s) 1198, 1395, 1476
BspCNI CTCAG 6 cut(s) 196, 771, 937, 1002, 1213, 1470
BspFNI CGCG 1 cut(s) 1099
BspLI GGNNCC 1 cut(s) 335
BspMI ACCTGC 1 cut(s) 1555
BspPI GGATC 2 cut(s) 402, 1310
BspTNI GGTCTC 2 cut(s) 491, 607
BsrI ACTGG 1 cut(s) 1091
BssECI CCNNGG 3 cut(s) 788, 1528, 1603
BssHII GCGCGC 1 cut(s) 1097
BssMI GATC 6 cut(s) 394, 754, 1264, 1315, 1560, 1690
BssNAI GTATAC 1 cut(s) 771
BssT1I CCWWGG 1 cut(s) 1603
Bst1107I GTATAC 1 cut(s) 771
Bst2UI CCWGG 1 cut(s) 870
Bst4CI ACNGT 1 cut(s) 263
Bst6I CTCTTC 7 cut(s) 261, 365, 489, 524, 618, 664, 917
BstAPI GCANNNNNTGC 1 cut(s) 1142
BstBAI YACGTR 1 cut(s) 892
BstC8I GCNNGC 2 cut(s) 1099, 1134
BstDEI CTNAG 6 cut(s) 204, 779, 945, 989, 1200, 1478
BstDSI CCRYGG 1 cut(s) 788
BstF5I GGATG 3 cut(s) 1010, 1162, 1582
BstFNI CGCG 1 cut(s) 1099
BstHHI GCGC 4 cut(s) 806, 1099, 1101, 1675
BstKTI GATC 6 cut(s) 397, 757, 1267, 1318, 1563, 1693
BstMAI GTCTC 2 cut(s) 491, 607
BstMBI GATC 6 cut(s) 394, 754, 1264, 1315, 1560, 1690
BstMWI GCNNNNNNNGC 3 cut(s) 1103, 1142, 1473
BstNI CCWGG 1 cut(s) 870
BstSCI CCNGG 1 cut(s) 868
BstSFI CTRYAG 1 cut(s) 195
BstSNI TACGTA 1 cut(s) 892
BstUI CGCG 1 cut(s) 1099
BstV1I GCAGC 5 cut(s) 337, 420, 980, 1129, 1173
BstV2I GAAGAC 1 cut(s) 308
BstX2I RGATCY 3 cut(s) 394, 754, 1690
BstXI CCANNNNNNTGG 3 cut(s) 242, 1017, 1291
BstYI RGATCY 3 cut(s) 394, 754, 1690
BstZ17I GTATAC 1 cut(s) 771
BsuRI GGCC 3 cut(s) 1198, 1395, 1476
BtgI CCRYGG 1 cut(s) 788
BtgZI GCGATG 1 cut(s) 1437
BtsCI GGATG 3 cut(s) 1010, 1162, 1582
BtsI GCAGTG 2 cut(s) 1220, 1296
BtsIMutI CAGTG 4 cut(s) 922, 1098, 1220, 1296
BveI ACCTGC 1 cut(s) 1555
Cac8I GCNNGC 2 cut(s) 1099, 1134
CaiI CAGNNNCTG 1 cut(s) 1142
CfoI GCGC 4 cut(s) 806, 1099, 1101, 1675
Cfr13I GGNCC 1 cut(s) 1250
CsiI ACCWGGT 1 cut(s) 868
Csp6I GTAC 1 cut(s) 1544
CviAII CATG 7 cut(s) 42, 52, 157, 328, 631, 691, 1503
CviQI GTAC 1 cut(s) 1544
DdeI CTNAG 6 cut(s) 204, 779, 945, 989, 1200, 1478
DpnI GATC 6 cut(s) 396, 756, 1266, 1317, 1562, 1692
DpnII GATC 6 cut(s) 394, 754, 1264, 1315, 1560, 1690
DraIII CACNNNGTG 1 cut(s) 789
Eam1104I CTCTTC 7 cut(s) 261, 365, 489, 524, 618, 664, 917
EarI CTCTTC 7 cut(s) 261, 365, 489, 524, 618, 664, 917
EciI GGCGGA 1 cut(s) 1656
Eco105I TACGTA 1 cut(s) 892
Eco130I CCWWGG 1 cut(s) 1603
Eco147I AGGCCT 1 cut(s) 1198
Eco31I GGTCTC 2 cut(s) 491, 607
Eco47I GGWCC 1 cut(s) 1250
Eco57I CTGAAG 5 cut(s) 6, 513, 678, 898, 1666
Eco88I CYCGRG 1 cut(s) 1385
EcoRII CCWGG 1 cut(s) 868
EcoT14I CCWWGG 1 cut(s) 1603
EcoT22I ATGCAT 1 cut(s) 1186
ErhI CCWWGG 1 cut(s) 1603
FaeI CATG 7 cut(s) 45, 55, 160, 331, 634, 694, 1506
FatI CATG 7 cut(s) 41, 51, 156, 327, 630, 690, 1502
FauNDI CATATG 1 cut(s) 1613
FblI GTMKAC 1 cut(s) 770
Fnu4HI GCNGC 6 cut(s) 351, 434, 663, 969, 1143, 1162
FokI GGATG 3 cut(s) 997, 1169, 1589
Fsp4HI GCNGC 6 cut(s) 351, 434, 663, 969, 1143, 1162
FspBI CTAG 5 cut(s) 225, 465, 957, 1107, 1340
GlaI GCGC 4 cut(s) 805, 1098, 1100, 1674
GluI GCNGC 6 cut(s) 351, 434, 663, 969, 1143, 1162
GsuI CTGGAG 1 cut(s) 1639
HaeIII GGCC 3 cut(s) 1198, 1395, 1476
HapII CCGG 1 cut(s) 1396
HhaI GCGC 4 cut(s) 806, 1099, 1101, 1675
Hin1II CATG 7 cut(s) 45, 55, 160, 331, 634, 694, 1506
Hin6I GCGC 4 cut(s) 804, 1097, 1099, 1673
HinP1I GCGC 4 cut(s) 804, 1097, 1099, 1673
HinfI GANTC 2 cut(s) 1052, 1608
HpaII CCGG 1 cut(s) 1396
HphI GGTGA 3 cut(s) 275, 284, 1187
Hpy166II GTNNAC 1 cut(s) 771
Hpy188I TCNGA 8 cut(s) 205, 573, 658, 846, 946, 1128, 1362, 1529
Hpy188III TCNNGA 3 cut(s) 129, 319, 398
Hpy8I GTNNAC 1 cut(s) 771
HpyAV CCTTC 7 cut(s) 48, 245, 466, 511, 1289, 1374, 1531
HpyCH4III ACNGT 1 cut(s) 263
HpyCH4IV ACGT 2 cut(s) 551, 891
HpyF10VI GCNNNNNNNGC 3 cut(s) 1103, 1142, 1473
HpyF3I CTNAG 6 cut(s) 204, 779, 945, 989, 1200, 1478
HpySE526I ACGT 2 cut(s) 551, 891
Hsp92II CATG 7 cut(s) 45, 55, 160, 331, 634, 694, 1506
HspAI GCGC 4 cut(s) 804, 1097, 1099, 1673
Kzo9I GATC 6 cut(s) 394, 754, 1264, 1315, 1560, 1690
Lsp1109I GCAGC 5 cut(s) 337, 420, 980, 1129, 1173
LweI GCATC 1 cut(s) 1119
MabI ACCWGGT 1 cut(s) 868
MaeI CTAG 5 cut(s) 225, 465, 957, 1107, 1340
MaeII ACGT 2 cut(s) 551, 891
MaeIII GTNAC 3 cut(s) 961, 1175, 1351
MalI GATC 6 cut(s) 396, 756, 1266, 1317, 1562, 1692
MboI GATC 6 cut(s) 394, 754, 1264, 1315, 1560, 1690
MfeI CAATTG 4 cut(s) 342, 423, 618, 1015
MflI RGATCY 3 cut(s) 394, 754, 1690
MhlI GDGCHC 1 cut(s) 785
MluCI AATT 8 cut(s) 342, 423, 604, 618, 638, 761, 1015, 1238
MlyI GAGTC 1 cut(s) 1602
MmeI TCCRAC 3 cut(s) 342, 584, 1282
Mph1103I ATGCAT 1 cut(s) 1186
MroXI GAANNNNTTC 1 cut(s) 1056
MseI TTAA 4 cut(s) 438, 449, 519, 864
MspI CCGG 1 cut(s) 1396
MspR9I CCNGG 1 cut(s) 870
MunI CAATTG 4 cut(s) 342, 423, 618, 1015
MvaI CCWGG 1 cut(s) 870
MvnI CGCG 1 cut(s) 1099
MwoI GCNNNNNNNGC 3 cut(s) 1103, 1142, 1473
NdeI CATATG 1 cut(s) 1613
NdeII GATC 6 cut(s) 394, 754, 1264, 1315, 1560, 1690
NlaIII CATG 7 cut(s) 45, 55, 160, 331, 634, 694, 1506
NlaIV GGNNCC 1 cut(s) 335
NmuCI GTSAC 2 cut(s) 1175, 1351
NsiI ATGCAT 1 cut(s) 1186
PaeR7I CTCGAG 1 cut(s) 1385
PauI GCGCGC 1 cut(s) 1097
PceI AGGCCT 1 cut(s) 1198
PdmI GAANNNNTTC 1 cut(s) 1056
PfeI GAWTC 1 cut(s) 1052
PkrI GCNGC 6 cut(s) 352, 435, 664, 970, 1144, 1163
PleI GAGTC 1 cut(s) 1602
PpsI GAGTC 1 cut(s) 1602
Ppu21I YACGTR 1 cut(s) 892
Psp6I CCWGG 1 cut(s) 868
PspGI CCWGG 1 cut(s) 868
PspN4I GGNNCC 1 cut(s) 335
PspPI GGNCC 1 cut(s) 1250
PspXI VCTCGAGB 1 cut(s) 1385
PstNI CAGNNNCTG 1 cut(s) 1142
PsuI RGATCY 3 cut(s) 394, 754, 1690
PteI GCGCGC 1 cut(s) 1097
RsaI GTAC 1 cut(s) 1545
RsaNI GTAC 1 cut(s) 1544
SaqAI TTAA 4 cut(s) 438, 449, 519, 864
SatI GCNGC 6 cut(s) 351, 434, 663, 969, 1143, 1162
Sau3AI GATC 6 cut(s) 394, 754, 1264, 1315, 1560, 1690
Sau96I GGNCC 1 cut(s) 1250
SchI GAGTC 1 cut(s) 1602
ScrFI CCNGG 1 cut(s) 870
SduI GDGCHC 1 cut(s) 785
SexAI ACCWGGT 1 cut(s) 868
SfaNI GCATC 1 cut(s) 1119
SfcI CTRYAG 1 cut(s) 195
Sfr274I CTCGAG 1 cut(s) 1385
SinI GGWCC 1 cut(s) 1250
SlaI CTCGAG 1 cut(s) 1385
SmlI CTYRAG 3 cut(s) 127, 389, 1385
SmoI CTYRAG 3 cut(s) 127, 389, 1385
SnaBI TACGTA 1 cut(s) 892
SpeI ACTAGT 1 cut(s) 224
Sse9I AATT 8 cut(s) 342, 423, 604, 618, 638, 761, 1015, 1238
SseBI AGGCCT 1 cut(s) 1198
SsiI CCGC 2 cut(s) 662, 1641
SspMI CTAG 5 cut(s) 225, 465, 957, 1107, 1340
StuI AGGCCT 1 cut(s) 1198
StyD4I CCNGG 1 cut(s) 868
StyI CCWWGG 1 cut(s) 1603
TaaI ACNGT 1 cut(s) 263
TaiI ACGT 2 cut(s) 554, 894
TaqI TCGA 2 cut(s) 546, 1386
TasI AATT 8 cut(s) 342, 423, 604, 618, 638, 761, 1015, 1238
TauI GCSGC 1 cut(s) 665
TfiI GAWTC 1 cut(s) 1052
Tru1I TTAA 4 cut(s) 438, 449, 519, 864
Tru9I TTAA 4 cut(s) 438, 449, 519, 864
TscAI CASTG 4 cut(s) 922, 1098, 1227, 1296
TseFI GTSAC 2 cut(s) 1175, 1351
TseI GCWGC 5 cut(s) 350, 433, 968, 1142, 1161
Tsp45I GTSAC 2 cut(s) 1175, 1351
TspGWI ACGGA 1 cut(s) 777
TspRI CASTG 4 cut(s) 922, 1098, 1227, 1296
VpaK11BI GGWCC 1 cut(s) 1250
XapI RAATTY 1 cut(s) 604
XcmI CCANNNNNNNNNTGG 1 cut(s) 1244
XhoI CTCGAG 1 cut(s) 1385
XmiI GTMKAC 1 cut(s) 770
XmnI GAANNNNTTC 1 cut(s) 1056
XspI CTAG 5 cut(s) 225, 465, 957, 1107, 1340
Zsp2I ATGCAT 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.